Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0003661

Physical Location & Seq

Genomic Coordinates Forward (+)

23589187 .. 23614897

Gene Structure 25711 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023431

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4992 bp

ATGGTCCTGGTTGAAGGAGAGGTTGAGGAGCTGGGTTGGGGGAAGAGTCGGAGTTTGAGTTGGGTTTTGGGCCTGGGAGGGACAGTAGGAGTCGTCGGGGGCCCATCAATCGGTTCCAAAACTCAAAATTGTCGGCTATCGAATCGTTCTCTTAGTTGCAAAACATGTGGTCAGGGTGCGTTTAATTGCCGGGGTCATTTTGGCCATATTGACATCGATTTGCCCGTTTATAATCCCTTGTTGTTCAATGTTCTTTACAAACTTCTACAAAGAACCTGCTTATCCTGTCACCATTTTCGAACAAGCCGAGAGCTGGTTGAAAAATGTGTTTCCCAATTGGAACTCGTGATGAAAGGTGATGTTGTTGCGGCAAAAAGATTAGACTCTGACTCACCTGATGAATACTCATATCTTGGGGAGGACGGGGAAGGAATTGATATTGAGAGAGACAGGCATTCGAAGCCGGAAAGATGGACATCTCTTCAGTTGACAGAGGCAATGTCTATTTTGGATCGTTCTCTGGAGAAACAACAAAAGTGCAAGAATTGTAAATTCAAGAATCCTAAAATCACTAAACCGACTTTTGGTTGGTTTCAGATGAGTGGTGCTGGCAATGCTAGAGAAAATGTGATCAGAAAATGCAAGCTGGTAGGACCTTTAACTGTTCAAGAAGAGGGTGAATCTTGTGGTGACGATGTTGATGCAGGCATATCTGATATATCTTGTGCATCCTCTCTTGTGACTCAAGATACTTCAACGAAGAAGAGTAAAGGACAAGAGACCGTAGTGCCCAGTGAGTTCTTTAAGCAGAAAAAAGCCTTCTCGGGAGATCTTTTACCAACCCAGGTTAAAGAAATAATGAGGCTTTTGTGGACAAATGAGGCTCAACTCTGCTCTTTTCTTAGTGATATCCAATGCCAAGGAGTTGAAAGAAAAGAAGGTCACTCTATGTTTTTCTTAGAGACTGTTCTTGTACCACCAATCAAATTTCGTCCTCCAGCCCATAGTGGTGATTCTGTGATGGAGCATCCTCAGACTGTTTCATTAAGTAAGGTGGTTCTGACAAATACTTACTTGAAAGACGCTCTTGGTAAGAACAGACACTCACAGACAATTCTGCGTTGGAGGCAACTGCAAGATTGTATAAATCTAATGTTCGATAGCAAAACTGTTGCGGGTCAAGGTAAATCAGATGCTTCTGGAATATGTCAGTTGCTGGAGAAAAAGGAAGGCATGTTCCGTCAGAAAATGATGGGAAAGAGGGTTAATAATGCGTGCCGGTCTGTTATATCCCCAGATCCCTATTTAGCTGTTAATGAGATTGGAATTCCTCCGATCTTTGCATTGACATTAACATATCCTGAGAGAGTTACTCCATGGAATGTTTTCAAGTTGAGGAATGCTATTGTCAATGGTTCTGAAATCCATCCTGGAGCTACGCACTATATTGATAAAACATCTATTAAGTTGCCACAAAAGCGGAGGGAGCGCTTGTTATTGTCAAGAAAGTTACCTTCTTCAAAAGGGGCAGGGACACAACATGGGAAGGGATCTGACTATGAAAACAAAATTGTACTCCGTCACTTGCAAGATGGGGACATTGTTCTTGTTAATCGCCAGCCTACACTTCACAAACCCAGTATAATGGCTCATATAGTTCGTGTGTTGAAGGGGGAGAAAACATTACGCATGCACTATGCAAATTGCAGTACATACAATGCTGATTTTGATGGTGATGAAATGAATGTTCATTTCCCACAAGATGAAATTTCTCGCGCTGAAGCTTACAATATGGTGAACGCAAATAATCAATACGTAAAGCCCACTAGTGGTGATCCCATTCGAGCCTTGATTCAGGACCATGTTATAAGTGCTGTCCTCCTCACAAAAAAGGACACCTTTCTCCGCTCAGATGAATTTAATCAGCTTCTTTATAGTTCTTCTGTTTCAGCATGTGGTTCAGACTCTTTTTCTGGCAAGCCAGGTCAAAAGGTTCTAACGTCTAACTCTGAAGATGCAATGCAGCCCCTTCTTCCTGCAATTTGGAAACCAGTGCGTCTGTGGACTGGAAAGCAGGTCATCACTACGTTGCTAAACCATATTACAAGTGGTTCTCCACCACTTACTGTTGAAAAGGAAGCAAAACTCCCATCTGGGTTTTTCAACTGTAAGAATCTTGAAGAAGAAGAGAAATCTGATAAAGGTCAACAAAAGAAAGAGAAGAAGCCAAGAAAAGAAGAGGCTGGTAAAGATGATTCAAAGGAAAAAGAGGATCCAGATGAGAGCAAGTTGTTAATCTATAGAAACAACTTGGTGCGTGGCGTGATCGACAAGAACCAGTTTGGTGATTATGGTTTGGTCCATAACGTGCAGGAACTCTATGGTTCGGAAGCTGCAGGAAAATTGCTTTCTGTTTTTAGTCGTTTATATACTGTTTATTTACAGATGCATGGGTTCACTTGTGGAGTTGACGATCTTTTGCTAATACAAAGCAAAGACAAAAAAATGAAGGAACAACTTGAAAGTTGTGAAGAGCTTGGTGAAAAAGTTTTCCGTGAGTTTATTGGGGTCGGACAAGATGAAAAAAAAGATCCGGTGATACTGCAGTCGGATATTGAAGAATTTTTACGCAGTAATGGAGAATCTGTGACGGAAATGTTGGACAGGAAAATGACAAGTGAACTTAATAAGAAAATGTCCAAATCTGATGTCTTCAAACAATTGTTTCGGAAAGGGTTATCAAAGCCATCTGTTAAGAATTATATGTATCTTATGACAAGCTCTGGGGCTAAAGGAGGTGTGGCAAATCTCCAACAAATTGCTTCATACTTGGGTCAGCAAGAGCTAGAAGGGAAGCGAGTCCCTCAAATGGTTTCTGGAAAGACATTACCATGCTTCCCTCCATGGGATTGGTCACCTCGAGCTGGAGGTTTTATTATTGATCGCTTTCTTACCGGTCTTCATCCTCAGGAGTATTATTTTCACTGCATGGCTGGCCGTGAAGGGTTGGTTGATACAGCTGTCAAAACATCTCGTAGTGGATACTTGCAAAGATGCCTAATAAAAAATCTCGAATGCCTTAAGGTTTCTTATGACCATACTGTCCGTGATGCTGATGGTTCAATTATTCAATTCCGATATGGAGAGGATGGTATTGATGTCCATTTGACAAGCTTTATTAAAAAATTTGAAAAACTGGCAAATAATAAAGAAATGTTTGACAAGAAATTTGGTCGTCAAATTGACAAATTCAATCCCTATATTGATGAGTTGCCTCCCGCTCTCAAAAGAAAAGCAGAAATGTCAGTTCCTAATTTGTCTTTGAAGGAGAAAAATGCCAACTTGAAGTTAATGGAGCACAAATATCTCAGCAGTCTTGCGCAACCAGGAGAACCAGTTGGTATTCTTGCTGCTCAGTCTGTAGGAGAACCATCAACACAGATGACTTTGAATACCTTCCATAATGCTGGGCGAAGTGAAATGAATGTGACTCTTGGAATTCCACGGCTCCAAGAGATCTTGATGACTGCTTCAAATGATATCAAGACTCCAGTTATGACATGTCCATTGATAAAGGAGAAAGCGGGGGATGCTAAAAGTCTAGCTGATAGGTTGAAGAGGATCACTGTGGCAGACATAATAGAGAGCATGAAAGTAACTATTACACCATTCACAGATCAGTATCATCAGACTTGCCGTGCTTATGAACTGGAGATGACACTTTTTATACCAGAATATCTTTCGAAATTTTCAGAAGATTGGGAGGAACTCCTAGAGGTTAAGTTTGTTAGGGCTTTGGAGGATGCCATACAAAATCATCTGGTTTTGCTTTCTAAAATTAGTGGTATAAAAGATGTCAAGGCTGATTCACTACCGAAGGCATCAAATGAGACAGATGAAGATGTTTCTGACAACATATCCCAGCATGGTGTAGAAGATGATGACGATGGCGGTGCAGATGACAGTGAACATGGTGAAGGAGCTGAGGATTTTGGGTTGGATGCACAAAAACGAAAGCGGCAGGGCACAGATGAGATGGAGTATGAAGATGACTGTGAGGATGAAGTGCATGAAGGTCATCAGTCCGATGGATTTGAAAGCGAAATTGATAAGGTGGAAAGTGATATTGAAAGTGATAAGGATGGTGTGACGCGGATTTCAGATGCTAATGATGAAATGCCTGAAAGTCCTTTAGGTGGAGACACATCTAAGCCACGGTCGAAGGGAAAGAAGACCAAATCAAGGGACAAAATTAAAAAGAAGACCAGAGCAAAGCTAGTAAGAAAGGAGTATGACAGGGCAACTTTTGTGTCAGTCAAGGGGTTCCACTTTGAGATCCATTTTAAATTTACCAATGAACCTCATATTCTTTTGGATCAGATTGCTCGGAAGACAGCTCGAAATGTTTGTGTCAAGAGCTCTGGCAATATTTTGGATTGTAAAGATATTGATATTTATGGTAATGAAGATCAAGTTTTCTGCTCCTGGAATGATCCCAGAAATAAATTACCGGGTGAAAAGAATCCCACGGAAGAAGAAAAGAAGATGCGAGCCTTGCAGACAGCAGGGGTGGATTTTGCTACACTATGGAAACTGCAAGATGATCTTGATGTCAACTATATATATTCCAACAACATTCATGCAATGCTGAACACTTACGGAGTAGAAGCCGCCAGAGAAACTATCATCAAAGAAATAAGTAACGTCTTCAAGGTTTATGGGATATCTGTCAATATTAGACACCTTTCACTAATAGCCGACTACATGACTCATAGTGGCGGTTATCGTCCAATGAACAGATTTGGGGGTATAGCAGAGTCTGTATCTCCGATCAATAAAATGTCATTTGAGACGGCTTCCAAGTTCATTCGTGAAGCAGCATATCATGGGGAGACGGATGACTTGGAAACCCCTTCTGCTAGGATCTGTCTTGGGCTGCCAGTGAAAGTGGGTACTGGTTGTTTTGATTTGATGCAAAAGATGGAGATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005736 GO:0005911 GO:0006139 GO:0006351 GO:0006360 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009987 GO:0010467 GO:0016070 GO:0018130 GO:0019438 GO:0030054 GO:0030880 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055029 GO:0055044 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 map00230 map00240 map01100 map03020

Pfam Domains

Protein Families

RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1664

Amino Acids

186.72

Weight (kDa)

6.43

Isoelectric Point (pI)

42.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	47 - 416	1.5e-21	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	419 - 600	4.4e-58	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	604 - 826	2e-29	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	878 - 979	5.9e-20	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	986 - 1602	1.9e-84	RNA polymerase Rpb1, domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57660 AT3G57660
fragaria_vesca	FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g34330 FvH4_5g35030 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210 FvH4_6g23210
malus_domestica	MD12G1066600.v1.1 MD14G1066700.v1.1
prunus_persica	Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1 Prupe.7G067700_v2.0.a1
pyrus_communis	pycom12g05800 pycom14g05430
rosa_chinensis	RchiOBHm_Chr3g0481021
rosa_laevigata	RLG00000023431
rosa_multiflora	Rmu_sc0002927.1_g000058
rosa_roxburghii	Rroxscaffold_6G00400730
rosa_rugosa	Rorug03G0189100
rosa_samantha	Rh3AG240000 Rh3BG275100 Rh3CG270600 Rh3DG266500
rosa_wichuraiana	Rw0G008430 Rw3G021430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	231, 1868
AasI	GACNNNNNNGTC	1 cut(s)	3101
Acc16I	TGCGCA	1 cut(s)	3381
Acc36I	ACCTGC	2 cut(s)	284, 2065
AccBSI	CCGCTC	2 cut(s)	1908, 3281
AccII	CGCG	2 cut(s)	1776, 4153
AcoI	YGGCCR	2 cut(s)	202, 2995
AcuI	CTGAAG	3 cut(s)	467, 1800, 2031
AfaI	GTAC	4 cut(s)	975, 1575, 1711, 4954
AfeI	AGCGCT	1 cut(s)	1490
AfiI	CCNNNNNNNGG	9 cut(s)	110, 313, 584, 1829, 2869, 2905, 2924, 4196, 4242
AflII	CTTAAG	1 cut(s)	3080
AflIII	ACRYGT	2 cut(s)	164, 3560
AgeI	ACCGGT	1 cut(s)	2954
AhlI	ACTAGT	1 cut(s)	1826
AjnI	CCWGG	7 cut(s)	6, 72, 843, 1429, 1981, 3385, 4484
AloI	GAACNNNNNNTCC	2 cut(s)	1220, 1252
Alw21I	GWGCWC	2 cut(s)	3360, 4421
Alw26I	GTCTC	7 cut(s)	441, 773, 956, 3884, 4194, 4844, 4886
AlwNI	CAGNNNCTG	2 cut(s)	1216, 4820
Ama87I	CYCGRG	2 cut(s)	823, 2919
Aor51HI	AGCGCT	1 cut(s)	1490
AoxI	GGCC	4 cut(s)	70, 100, 202, 2995
ApaI	GGGCCC	1 cut(s)	104
ApeKI	GCWGC	5 cut(s)	2023, 2393, 3410, 4877, 4936
ArsI	GACNNNNNNTTYG	4 cut(s)	2694, 2726, 3212, 3244
AsiGI	ACCGGT	1 cut(s)	2954
Asp700I	GAANNNNTTC	2 cut(s)	1385, 3455
AspLEI	GCGC	3 cut(s)	1491, 1778, 3382
AspS9I	GGNCC	7 cut(s)	4, 70, 100, 101, 653, 1858, 2359
AsuC2I	CCSGG	2 cut(s)	191, 4512
AsuII	TTCGAA	3 cut(s)	298, 458, 3743
AvaI	CYCGRG	2 cut(s)	823, 2919
AvaII	GGWCC	4 cut(s)	4, 653, 1858, 2359
AxyI	CCTNAGG	1 cut(s)	2967
BaeGI	GKGCMC	3 cut(s)	104, 792, 4028
BaeI	ACNNNNGTAYC	4 cut(s)	2591, 2624, 3006, 3039
BalI	TGGCCA	1 cut(s)	204
BamHI	GGATCC	1 cut(s)	2272
BanII	GRGCYC	2 cut(s)	104, 4421
BarI	GAAGNNNNNNTAC	2 cut(s)	2610, 2642
BauI	CACGAG	1 cut(s)	344
BbsI	GAAGAC	6 cut(s)	2704, 2951, 4238, 4268, 4397, 4699
Bbv12I	GWGCWC	2 cut(s)	3360, 4421
BbvCI	CCTCAGC	1 cut(s)	3984
BbvI	GCAGC	5 cut(s)	2035, 2380, 3397, 4889, 4923
BceAI	ACGGC	4 cut(s)	2982, 3521, 3681, 4869
BcgI	CGANNNNNNTGC	4 cut(s)	683, 717, 3935, 3969
BciT130I	CCWGG	7 cut(s)	8, 74, 845, 1431, 1983, 3387, 4486
BciVI	GTATCC	1 cut(s)	3035
BclI	TGATCA	1 cut(s)	630
BcnI	CCSGG	2 cut(s)	191, 4512
BcoDI	GTCTC	7 cut(s)	441, 773, 956, 3884, 4194, 4844, 4886
BcuI	ACTAGT	1 cut(s)	1826
BfaI	CTAG	7 cut(s)	618, 1827, 2846, 3602, 3773, 4277, 4920
BfmI	CTRYAG	4 cut(s)	2299, 2394, 2603, 3420
BfoI	RGCGCY	1 cut(s)	1492
BfrI	CTTAAG	1 cut(s)	3080
BfuAI	ACCTGC	2 cut(s)	284, 2065
BfuI	GTATCC	1 cut(s)	3035
BglII	AGATCT	2 cut(s)	829, 3516
BisI	GCNGC	8 cut(s)	369, 2024, 2394, 3411, 4019, 4671, 4878, 4937
BlsI	GCNGC	8 cut(s)	370, 2025, 2395, 3412, 4020, 4672, 4879, 4938
Bme1390I	CCNGG	9 cut(s)	8, 74, 191, 845, 1431, 1983, 3387, 4486, 4512
Bme18I	GGWCC	4 cut(s)	4, 653, 1858, 2359
BmeT110I	CYCGRG	2 cut(s)	823, 2919
BmgT120I	GGNCC	7 cut(s)	4, 70, 100, 101, 653, 1858, 2359
BmiI	GGNNCC	6 cut(s)	101, 102, 115, 2274, 3509, 4325
BmrFI	CCNGG	9 cut(s)	8, 74, 191, 845, 1431, 1983, 3387, 4486, 4512
BmrI	ACTGGG	2 cut(s)	786, 1632
BmuI	ACTGGG	2 cut(s)	786, 1632
BpiI	GAAGAC	6 cut(s)	2704, 2951, 4238, 4268, 4397, 4699
BplI	GAGNNNNNCTC	2 cut(s)	1357, 1389
BpmI	CTGGAG	7 cut(s)	542, 981, 1238, 1452, 2946, 3534, 3731
Bpu10I	CCTNAGC	1 cut(s)	3984
Bpu14I	TTCGAA	3 cut(s)	298, 458, 3743
BpuEI	CTTGAG	1 cut(s)	729
BpuMI	CCSGG	2 cut(s)	191, 4512
Bsa29I	ATCGAT	1 cut(s)	216
BsaAI	YACGTR	1 cut(s)	1816
BsaBI	GATNNNNATC	2 cut(s)	475, 3681
BsaI	GGTCTC	1 cut(s)	773
BsaJI	CCNNGG	9 cut(s)	73, 190, 843, 919, 1376, 2903, 3503, 4214, 4527
BsaWI	WCCGGW	2 cut(s)	2593, 2954
Bsc4I	CCNNNNNNNGG	9 cut(s)	110, 313, 584, 1829, 2869, 2905, 2924, 4196, 4242
Bse118I	RCCGGY	2 cut(s)	1278, 2954
Bse21I	CCTNAGG	1 cut(s)	2967
Bse3DI	GCAATG	4 cut(s)	504, 619, 2025, 4650
Bse8I	GATNNNNATC	2 cut(s)	475, 3681
BseBI	CCWGG	7 cut(s)	8, 74, 845, 1431, 1983, 3387, 4486
BseCI	ATCGAT	1 cut(s)	216
BseDI	CCNNGG	9 cut(s)	73, 190, 843, 919, 1376, 2903, 3503, 4214, 4527
BseJI	GATNNNNATC	2 cut(s)	475, 3681
BseLI	CCNNNNNNNGG	9 cut(s)	110, 313, 584, 1829, 2869, 2905, 2924, 4196, 4242
BseMI	GCAATG	4 cut(s)	504, 619, 2025, 4650
BseMII	CTCAG	7 cut(s)	1046, 1353, 1923, 2981, 3382, 3428, 3975
BseRI	GAGGAG	2 cut(s)	41, 1871
BseSI	GKGCMC	3 cut(s)	104, 792, 4028
BseXI	GCAGC	5 cut(s)	2035, 2380, 3397, 4889, 4923
BseYI	CCCAGC	3 cut(s)	31, 3467, 3921
BsgI	GTGCAG	2 cut(s)	2390, 3975
Bsh1236I	CGCG	2 cut(s)	1776, 4153
Bsh1285I	CGRYCG	1 cut(s)	4220
BshFI	GGCC	4 cut(s)	72, 102, 204, 2997
BshTI	ACCGGT	1 cut(s)	2954
BshVI	ATCGAT	1 cut(s)	216
BsiEI	CGRYCG	1 cut(s)	4220
BsiHKAI	GWGCWC	2 cut(s)	3360, 4421
BsiHKCI	CYCGRG	2 cut(s)	823, 2919
BsiSI	CCGG	6 cut(s)	190, 464, 1279, 2594, 2955, 4511
BslFI	GGGAC	5 cut(s)	94, 1546, 1610, 2846, 4259
BslI	CCNNNNNNNGG	9 cut(s)	110, 313, 584, 1829, 2869, 2905, 2924, 4196, 4242
BsmAI	GTCTC	7 cut(s)	441, 773, 956, 3884, 4194, 4844, 4886
BsmBI	CGTCTC	2 cut(s)	4844, 4886
BsmFI	GGGAC	5 cut(s)	94, 1546, 1610, 2846, 4259
BsmI	GAATGC	3 cut(s)	454, 1405, 3080
BsnI	GGCC	4 cut(s)	72, 102, 204, 2997
Bso31I	GGTCTC	1 cut(s)	773
BsoBI	CYCGRG	2 cut(s)	823, 2919
Bsp119I	TTCGAA	3 cut(s)	298, 458, 3743
Bsp120I	GGGCCC	1 cut(s)	100
Bsp1286I	GDGCHC	5 cut(s)	104, 792, 3360, 4028, 4421
Bsp19I	CCATGG	2 cut(s)	1376, 2903
BspANI	GGCC	4 cut(s)	72, 102, 204, 2997
BspCNI	CTCAG	7 cut(s)	1045, 1354, 1922, 2980, 3381, 3427, 3976
BspDI	ATCGAT	1 cut(s)	216
BspFNI	CGCG	2 cut(s)	1776, 4153
BspLI	GGNNCC	6 cut(s)	101, 102, 115, 2274, 3509, 4325
BspMAI	CTGCAG	2 cut(s)	2398, 2607
BspMI	ACCTGC	2 cut(s)	284, 2065
BspQI	GCTCTTC	1 cut(s)	2526
BspT104I	TTCGAA	3 cut(s)	298, 458, 3743
BspTI	CTTAAG	1 cut(s)	3080
BspTNI	GGTCTC	1 cut(s)	773
BsrBI	CCGCTC	2 cut(s)	1908, 3281
BsrDI	GCAATG	4 cut(s)	504, 619, 2025, 4650
BsrFI	RCCGGY	2 cut(s)	1278, 2954
BssAI	RCCGGY	2 cut(s)	1278, 2954
BssECI	CCNNGG	9 cut(s)	73, 190, 843, 919, 1376, 2903, 3503, 4214, 4527
BssSI	CACGAG	1 cut(s)	344
BssT1I	CCWWGG	3 cut(s)	919, 1376, 2903
Bst2BI	CACGAG	1 cut(s)	344
Bst2UI	CCWGG	7 cut(s)	8, 74, 845, 1431, 1983, 3387, 4486
Bst6I	CTCTTC	8 cut(s)	38, 486, 666, 758, 2181, 2232, 2526, 3611
BstAFI	CTTAAG	1 cut(s)	3080
BstBAI	YACGTR	1 cut(s)	1816
BstBI	TTCGAA	3 cut(s)	298, 458, 3743
BstC8I	GCNNGC	9 cut(s)	610, 644, 706, 1276, 1619, 1691, 1979, 2995, 4551
BstDSI	CCRYGG	5 cut(s)	1376, 2903, 3503, 4214, 4527
BstEII	GGTNACC	1 cut(s)	2913
BstFNI	CGCG	2 cut(s)	1776, 4153
BstH2I	RGCGCY	1 cut(s)	1492
BstHHI	GCGC	3 cut(s)	1491, 1778, 3382
BstMAI	GTCTC	7 cut(s)	441, 773, 956, 3884, 4194, 4844, 4886
BstMCI	CGRYCG	1 cut(s)	4220
BstMWI	GCNNNNNNNGC	8 cut(s)	460, 614, 1126, 1477, 1486, 2994, 3590, 4555
BstNI	CCWGG	7 cut(s)	8, 74, 845, 1431, 1983, 3387, 4486
BstNSI	RCATGY	5 cut(s)	168, 1237, 1693, 1956, 3564
BstPI	GGTNACC	1 cut(s)	2913
BstSCI	CCNGG	9 cut(s)	6, 72, 189, 843, 1429, 1981, 3385, 4484, 4510
BstSFI	CTRYAG	4 cut(s)	2299, 2394, 2603, 3420
BstSLI	GKGCMC	3 cut(s)	104, 792, 4028
BstSNI	TACGTA	1 cut(s)	1816
BstUI	CGCG	2 cut(s)	1776, 4153
BstV1I	GCAGC	5 cut(s)	2035, 2380, 3397, 4889, 4923
BstV2I	GAAGAC	6 cut(s)	2704, 2951, 4238, 4268, 4397, 4699
BstX2I	RGATCY	8 cut(s)	829, 1297, 1550, 2272, 2590, 3516, 4335, 4923
BstXI	CCANNNNNNTGG	3 cut(s)	1645, 2910, 3467
BstYI	RGATCY	8 cut(s)	829, 1297, 1550, 2272, 2590, 3516, 4335, 4923
Bsu15I	ATCGAT	1 cut(s)	216
Bsu36I	CCTNAGG	1 cut(s)	2967
BsuI	GTATCC	1 cut(s)	3035
BsuRI	GGCC	4 cut(s)	72, 102, 204, 2997
BsuTUI	ATCGAT	1 cut(s)	216
BtgI	CCRYGG	5 cut(s)	1376, 2903, 3503, 4214, 4527
BtsI	GCAGTG	1 cut(s)	2983
BtsIMutI	CAGTG	6 cut(s)	799, 2058, 2983, 3624, 3970, 4947
BveI	ACCTGC	2 cut(s)	284, 2065
Cac8I	GCNNGC	9 cut(s)	610, 644, 706, 1276, 1619, 1691, 1979, 2995, 4551
CaiI	CAGNNNCTG	2 cut(s)	1216, 4820
CfoI	GCGC	3 cut(s)	1491, 1778, 3382
Cfr10I	RCCGGY	2 cut(s)	1278, 2954
Cfr13I	GGNCC	7 cut(s)	4, 70, 100, 101, 653, 1858, 2359
ClaI	ATCGAT	1 cut(s)	216
CseI	GACGC	3 cut(s)	1091, 2045, 4159
Csp6I	GTAC	4 cut(s)	974, 1574, 1710, 4953
CspAI	ACCGGT	1 cut(s)	2954
CspCI	CAANNNNNGTGG	2 cut(s)	148, 183
CviQI	GTAC	4 cut(s)	974, 1574, 1710, 4953
DraI	TTTAAA	1 cut(s)	4345
DrdI	GACNNNNNNGTC	1 cut(s)	3101
DseDI	GACNNNNNNGTC	1 cut(s)	3101
EaeI	YGGCCR	2 cut(s)	202, 2995
Eam1104I	CTCTTC	8 cut(s)	38, 486, 666, 758, 2181, 2232, 2526, 3611
EarI	CTCTTC	8 cut(s)	38, 486, 666, 758, 2181, 2232, 2526, 3611
Ecl136II	GAGCTC	1 cut(s)	4419
Eco105I	TACGTA	1 cut(s)	1816
Eco130I	CCWWGG	3 cut(s)	919, 1376, 2903
Eco24I	GRGCYC	2 cut(s)	104, 4421
Eco31I	GGTCTC	1 cut(s)	773
Eco32I	GATATC	3 cut(s)	910, 3541, 4725
Eco47I	GGWCC	4 cut(s)	4, 653, 1858, 2359
Eco47III	AGCGCT	1 cut(s)	1490
Eco53kI	GAGCTC	1 cut(s)	4419
Eco57I	CTGAAG	3 cut(s)	467, 1800, 2031
Eco81I	CCTNAGG	1 cut(s)	2967
Eco88I	CYCGRG	2 cut(s)	823, 2919
Eco91I	GGTNACC	1 cut(s)	2913
EcoICRI	GAGCTC	1 cut(s)	4419
EcoO109I	RGGNCCY	2 cut(s)	100, 653
EcoO65I	GGTNACC	1 cut(s)	2913
EcoRI	GAATTC	2 cut(s)	1326, 3498
EcoRII	CCWGG	7 cut(s)	6, 72, 843, 1429, 1981, 3385, 4484
EcoRV	GATATC	3 cut(s)	910, 3541, 4725
EcoT14I	CCWWGG	3 cut(s)	919, 1376, 2903
EcoT22I	ATGCAT	1 cut(s)	2451
EcoT38I	GRGCYC	2 cut(s)	104, 4421
ErhI	CCWWGG	3 cut(s)	919, 1376, 2903
Esp3I	CGTCTC	2 cut(s)	4844, 4886
FalI	AAGNNNNNCTT	6 cut(s)	1071, 1103, 1883, 1915, 4590, 4622
FaqI	GGGAC	5 cut(s)	94, 1546, 1610, 2846, 4259
FauI	CCCGC	3 cut(s)	1168, 3286, 3577
FbaI	TGATCA	1 cut(s)	630
Fnu4HI	GCNGC	8 cut(s)	369, 2024, 2394, 3411, 4019, 4671, 4878, 4937
FriOI	GRGCYC	2 cut(s)	104, 4421
Fsp4HI	GCNGC	8 cut(s)	369, 2024, 2394, 3411, 4019, 4671, 4878, 4937
FspBI	CTAG	7 cut(s)	618, 1827, 2846, 3602, 3773, 4277, 4920
FspI	TGCGCA	1 cut(s)	3381
GlaI	GCGC	3 cut(s)	1490, 1777, 3381
GluI	GCNGC	8 cut(s)	369, 2024, 2394, 3411, 4019, 4671, 4878, 4937
GsaI	CCCAGC	3 cut(s)	35, 3471, 3925
GsuI	CTGGAG	7 cut(s)	542, 981, 1238, 1452, 2946, 3534, 3731
HaeII	RGCGCY	1 cut(s)	1492
HaeIII	GGCC	4 cut(s)	72, 102, 204, 2997
HapII	CCGG	6 cut(s)	190, 464, 1279, 2594, 2955, 4511
HgaI	GACGC	3 cut(s)	1091, 2045, 4159
HhaI	GCGC	3 cut(s)	1491, 1778, 3382
Hin6I	GCGC	3 cut(s)	1489, 1776, 3380
HinP1I	GCGC	3 cut(s)	1489, 1776, 3380
HincII	GTYRAC	4 cut(s)	489, 2207, 2470, 4615
HindII	GTYRAC	4 cut(s)	489, 2207, 2470, 4615
HindIII	AAGCTT	2 cut(s)	1782, 3172
HpaII	CCGG	6 cut(s)	190, 464, 1279, 2594, 2955, 4511
Hpy99I	CGWCG	1 cut(s)	98
HpyCH4IV	ACGT	5 cut(s)	1815, 2000, 2087, 2367, 4704
HpyF10VI	GCNNNNNNNGC	8 cut(s)	460, 614, 1126, 1477, 1486, 2994, 3590, 4555
HpySE526I	ACGT	5 cut(s)	1815, 2000, 2087, 2367, 4704
HspAI	GCGC	3 cut(s)	1489, 1776, 3380
Ksp22I	TGATCA	1 cut(s)	630
LguI	GCTCTTC	1 cut(s)	2526
LmnI	GCTCC	8 cut(s)	28, 1024, 1433, 1486, 3355, 3513, 3980, 4487
Lsp1109I	GCAGC	5 cut(s)	2035, 2380, 3397, 4889, 4923
MaeI	CTAG	7 cut(s)	618, 1827, 2846, 3602, 3773, 4277, 4920
MaeII	ACGT	5 cut(s)	1815, 2000, 2087, 2367, 4704
MbiI	CCGCTC	2 cut(s)	1908, 3281
MfeI	CAATTG	2 cut(s)	335, 2720
MflI	RGATCY	8 cut(s)	829, 1297, 1550, 2272, 2590, 3516, 4335, 4923
MhlI	GDGCHC	5 cut(s)	104, 792, 3360, 4028, 4421
MlsI	TGGCCA	1 cut(s)	204
MluNI	TGGCCA	1 cut(s)	204
MmeI	TCCRAC	8 cut(s)	29, 1103, 2551, 2589, 2640, 2836, 3978, 4653
Mox20I	TGGCCA	1 cut(s)	204
Mph1103I	ATGCAT	1 cut(s)	2451
MroXI	GAANNNNTTC	2 cut(s)	1385, 3455
MscI	TGGCCA	1 cut(s)	204
MslI	CAYNNNNRTG	2 cut(s)	1008, 2890
Msp20I	TGGCCA	1 cut(s)	204
MspA1I	CMGCKG	1 cut(s)	3020
MspCI	CTTAAG	1 cut(s)	3080
MspI	CCGG	6 cut(s)	190, 464, 1279, 2594, 2955, 4511
MspR9I	CCNGG	9 cut(s)	8, 74, 191, 845, 1431, 1983, 3387, 4486, 4512
MunI	CAATTG	2 cut(s)	335, 2720
Mva1269I	GAATGC	3 cut(s)	454, 1405, 3080
MvaI	CCWGG	7 cut(s)	8, 74, 845, 1431, 1983, 3387, 4486
MvnI	CGCG	2 cut(s)	1776, 4153
MwoI	GCNNNNNNNGC	8 cut(s)	460, 614, 1126, 1477, 1486, 2994, 3590, 4555
NciI	CCSGG	2 cut(s)	191, 4512
NcoI	CCATGG	2 cut(s)	1376, 2903
NlaIV	GGNNCC	6 cut(s)	101, 102, 115, 2274, 3509, 4325
NmeAIII	GCCGAG	1 cut(s)	332
NmuCI	GTSAC	9 cut(s)	287, 689, 739, 941, 1580, 2647, 2913, 3487, 4147
NsbI	TGCGCA	1 cut(s)	3381
NsiI	ATGCAT	1 cut(s)	2451
NspI	RCATGY	5 cut(s)	168, 1237, 1693, 1956, 3564
NspV	TTCGAA	3 cut(s)	298, 458, 3743
PaeI	GCATGC	1 cut(s)	1693
PaeR7I	CTCGAG	1 cut(s)	2919
PciI	ACATGT	2 cut(s)	164, 3560
PciSI	GCTCTTC	1 cut(s)	2526
PcsI	WCGNNNNNNNCGW	2 cut(s)	222, 304
PctI	GAATGC	3 cut(s)	454, 1405, 3080
PdmI	GAANNNNTTC	2 cut(s)	1385, 3455
PfoI	TCCNGGA	2 cut(s)	1429, 4484
PinAI	ACCGGT	1 cut(s)	2954
PkrI	GCNGC	8 cut(s)	370, 2025, 2395, 3412, 4020, 4672, 4879, 4938
Ppu21I	YACGTR	1 cut(s)	1816
PpuMI	RGGWCCY	1 cut(s)	653
PscI	ACATGT	2 cut(s)	164, 3560
PsiI	TTATAA	2 cut(s)	231, 1868
Psp124BI	GAGCTC	1 cut(s)	4421
Psp5II	RGGWCCY	1 cut(s)	653
Psp6I	CCWGG	7 cut(s)	6, 72, 843, 1429, 1981, 3385, 4484
PspEI	GGTNACC	1 cut(s)	2913
PspFI	CCCAGC	3 cut(s)	31, 3467, 3921
PspGI	CCWGG	7 cut(s)	6, 72, 843, 1429, 1981, 3385, 4484
PspN4I	GGNNCC	6 cut(s)	101, 102, 115, 2274, 3509, 4325
PspOMI	GGGCCC	1 cut(s)	100
PspPI	GGNCC	7 cut(s)	4, 70, 100, 101, 653, 1858, 2359
PspPPI	RGGWCCY	1 cut(s)	653
PspXI	VCTCGAGB	1 cut(s)	2919
PstI	CTGCAG	2 cut(s)	2398, 2607
PstNI	CAGNNNCTG	2 cut(s)	1216, 4820
PsuI	RGATCY	8 cut(s)	829, 1297, 1550, 2272, 2590, 3516, 4335, 4923
PvuII	CAGCTG	1 cut(s)	3020
RsaI	GTAC	4 cut(s)	975, 1575, 1711, 4954
RsaNI	GTAC	4 cut(s)	974, 1574, 1710, 4953
RseI	CAYNNNNRTG	2 cut(s)	1008, 2890
SacI	GAGCTC	1 cut(s)	4421
SapI	GCTCTTC	1 cut(s)	2526
SatI	GCNGC	8 cut(s)	369, 2024, 2394, 3411, 4019, 4671, 4878, 4937
Sau96I	GGNCC	7 cut(s)	4, 70, 100, 101, 653, 1858, 2359
ScrFI	CCNGG	9 cut(s)	8, 74, 191, 845, 1431, 1983, 3387, 4486, 4512
SduI	GDGCHC	5 cut(s)	104, 792, 3360, 4028, 4421
SfcI	CTRYAG	4 cut(s)	2299, 2394, 2603, 3420
Sfr274I	CTCGAG	1 cut(s)	2919
SfuI	TTCGAA	3 cut(s)	298, 458, 3743
SinI	GGWCC	4 cut(s)	4, 653, 1858, 2359
SlaI	CTCGAG	1 cut(s)	2919
SmiMI	CAYNNNNRTG	2 cut(s)	1008, 2890
SmlI	CTYRAG	3 cut(s)	744, 2919, 3080
SmoI	CTYRAG	3 cut(s)	744, 2919, 3080
SnaBI	TACGTA	1 cut(s)	1816
SpeI	ACTAGT	1 cut(s)	1826
SphI	GCATGC	1 cut(s)	1693
SspI	AATATT	2 cut(s)	4429, 4735
SspMI	CTAG	7 cut(s)	618, 1827, 2846, 3602, 3773, 4277, 4920
SstI	GAGCTC	1 cut(s)	4421
StyD4I	CCNGG	9 cut(s)	6, 72, 189, 843, 1429, 1981, 3385, 4484, 4510
StyI	CCWWGG	3 cut(s)	919, 1376, 2903
TaiI	ACGT	5 cut(s)	1818, 2003, 2090, 2370, 4707
TatI	WGTACW	2 cut(s)	1573, 1709
TauI	GCSGC	3 cut(s)	371, 4021, 4673
TscAI	CASTG	6 cut(s)	799, 2058, 2990, 3631, 3970, 4947
TseFI	GTSAC	9 cut(s)	287, 689, 739, 941, 1580, 2647, 2913, 3487, 4147
TseI	GCWGC	5 cut(s)	2023, 2393, 3410, 4877, 4936
Tsp45I	GTSAC	9 cut(s)	287, 689, 739, 941, 1580, 2647, 2913, 3487, 4147
TspGWI	ACGGA	8 cut(s)	1229, 1568, 2543, 2666, 3095, 4544, 4674, 4910
TspRI	CASTG	6 cut(s)	799, 2058, 2990, 3631, 3970, 4947
Vha464I	CTTAAG	1 cut(s)	3080
VpaK11BI	GGWCC	4 cut(s)	4, 653, 1858, 2359
XceI	RCATGY	5 cut(s)	168, 1237, 1693, 1956, 3564
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2058, 2105
XhoI	CTCGAG	1 cut(s)	2919
XmnI	GAANNNNTTC	2 cut(s)	1385, 3455
XspI	CTAG	7 cut(s)	618, 1827, 2846, 3602, 3773, 4277, 4920
Zsp2I	ATGCAT	1 cut(s)	2451

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.