AT3G59390

Uncharacterized conserved protein (DUF2054)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005830
Physical Location & Seq
Forward (+)
21946086 .. 21948372
2287 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G59390.2

Sequence Viewer

Length: 822 bp
ATGTCCAAATCTGGGTTTAGTCTGCTGTTCCTCGTTCTTCTACTTATCATGGGTTTCTTATTTCCGTTGAGAGTTTCTGCAATTAGGAAGGATATCGGATTTTTGGAAGAGCGGAGTTGCAGAACAACTGTCCAAGGAAGATACTTGATCTCCGATGATGAAGGCAATGTGTGTGATGCTTTGTCATTGGAATCTCGAACTCGTTGTTGTCCATGGAAAGGAGAAAGATTCTCTTGTCATGGATGCAACATACTTTCACAATGCTGCAACTCTTATGAATTTTGCGTTTCTTGCTGCTTGAATCCGTCTCAGACGCTTCTTGAGAAGGTGGTTAAGGTCAAAGTTGCAAAGCCGGCTACATCAGGGACTTACAAGAGTGTATTTGATTTTTGTGCCGGGAGATGCCGGCATAACTCTGAGAGTGTGGTTCACGAAAATGCTTACCACAGCGAGTTTCACCATTGTTTTTCTCTGACATCAAATGCTTCAGGTGCTAATCTTACACAAGTTGAAACAAGACTTCTTGGTATTGACGTTATTGTCGGAAGTCAAGGAGATTCATGCGATGCAGTTTGCAAATCACGTGGACAATTGTGTGTGATGAATAAACTCTCACTTCTAAACCAGTGTGACGTTATGAAAAGATACATGAACTGCAAAGGGTCGTGTTTAGCAAGTGCCGGGGCTGACCAACCTGCTGAAGTTGTCGAAGATGCGCCGAGAGATTTGTATCCAGGCGCGTGTTTGTATACCCGGACACAATCATTGCTTTCCTGTGACGGTTCACACCAACACACCCGACGACTCTGCCCATGTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2054 Glyco_transf_18

Protein Analysis

273

Amino Acids

30.03

Weight (kDa)

7.96

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPRING1 PF10218 37 - 156 1.8e-37 SREBP regulating gene protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 539
Acc36I ACCTGC 1 cut(s) 703
AccBSI CCGCTC 1 cut(s) 112
AccI GTMKAC 1 cut(s) 749
AccII CGCG 1 cut(s) 740
AciI CCGC 1 cut(s) 112
AcsI RAATTY 1 cut(s) 278
AcuI CTGAAG 2 cut(s) 471, 720
AcvI CACGTG 1 cut(s) 584
AfiI CCNNNNNNNGG 2 cut(s) 12, 218
AgsI TTSAA 2 cut(s) 301, 512
AjnI CCWGG 1 cut(s) 733
Alw26I GTCTC 1 cut(s) 312
ApeKI GCWGC 2 cut(s) 264, 294
ApoI RAATTY 1 cut(s) 278
AspLEI GCGC 2 cut(s) 718, 740
AsuC2I CCSGG 3 cut(s) 397, 682, 754
AsuHPI GGTGA 1 cut(s) 449
BbrPI CACGTG 1 cut(s) 584
BbvI GCAGC 2 cut(s) 251, 281
BcgI CGANNNNNNTGC 1 cut(s) 789
BciT130I CCWGG 1 cut(s) 735
BciVI GTATCC 1 cut(s) 741
BcnI CCSGG 3 cut(s) 397, 682, 754
BcoDI GTCTC 1 cut(s) 312
BfaI CTAG 1 cut(s) 820
BfuAI ACCTGC 1 cut(s) 703
BfuI GTATCC 1 cut(s) 741
BisI GCNGC 2 cut(s) 265, 295
BlsI GCNGC 2 cut(s) 266, 296
Bme1390I CCNGG 4 cut(s) 397, 682, 735, 754
BmrFI CCNGG 4 cut(s) 397, 682, 735, 754
BmsI GCATC 5 cut(s) 166, 233, 392, 556, 703
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 3 cut(s) 397, 682, 754
BsaAI YACGTR 1 cut(s) 584
BsaBI GATNNNNATC 1 cut(s) 729
BsaJI CCNNGG 3 cut(s) 133, 212, 681
BsaXI ACNNNNNCTCC 2 cut(s) 134, 164
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 218
Bse118I RCCGGY 2 cut(s) 352, 405
Bse1I ACTGG 1 cut(s) 625
Bse3DI GCAATG 2 cut(s) 172, 764
Bse8I GATNNNNATC 1 cut(s) 729
BseBI CCWGG 1 cut(s) 735
BseDI CCNNGG 3 cut(s) 133, 212, 681
BseGI GGATG 1 cut(s) 248
BseJI GATNNNNATC 1 cut(s) 729
BseLI CCNNNNNNNGG 2 cut(s) 12, 218
BseMI GCAATG 2 cut(s) 172, 764
BseMII CTCAG 2 cut(s) 323, 408
BseNI ACTGG 1 cut(s) 625
BseXI GCAGC 2 cut(s) 251, 281
Bsh1236I CGCG 1 cut(s) 740
BsiSI CCGG 5 cut(s) 353, 396, 406, 681, 754
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 12, 218
BsmAI GTCTC 1 cut(s) 312
BsmBI CGTCTC 1 cut(s) 312
BsmFI GGGAC 1 cut(s) 379
Bsp143I GATC 1 cut(s) 147
Bsp19I CCATGG 1 cut(s) 212
BspACI CCGC 1 cut(s) 112
BspCNI CTCAG 2 cut(s) 322, 409
BspFNI CGCG 1 cut(s) 740
BspMI ACCTGC 1 cut(s) 703
BspQI GCTCTTC 1 cut(s) 102
BsrBI CCGCTC 1 cut(s) 112
BsrDI GCAATG 2 cut(s) 172, 764
BsrFI RCCGGY 2 cut(s) 352, 405
BsrI ACTGG 1 cut(s) 625
BssAI RCCGGY 2 cut(s) 352, 405
BssECI CCNNGG 3 cut(s) 133, 212, 681
BssMI GATC 1 cut(s) 147
BssNAI GTATAC 1 cut(s) 750
BssT1I CCWWGG 2 cut(s) 133, 212
Bst1107I GTATAC 1 cut(s) 750
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 2 cut(s) 130, 782
Bst6I CTCTTC 1 cut(s) 102
BstBAI YACGTR 1 cut(s) 584
BstC8I GCNNGC 2 cut(s) 354, 407
BstDEI CTNAG 2 cut(s) 309, 417
BstDSI CCRYGG 1 cut(s) 212
BstF5I GGATG 1 cut(s) 248
BstFNI CGCG 1 cut(s) 740
BstHHI GCGC 2 cut(s) 718, 740
BstKTI GATC 1 cut(s) 150
BstMAI GTCTC 1 cut(s) 312
BstMBI GATC 1 cut(s) 147
BstMWI GCNNNNNNNGC 3 cut(s) 291, 353, 491
BstNI CCWGG 1 cut(s) 735
BstSCI CCNGG 4 cut(s) 395, 680, 733, 752
BstUI CGCG 1 cut(s) 740
BstV1I GCAGC 2 cut(s) 251, 281
BstZ17I GTATAC 1 cut(s) 750
BsuI GTATCC 1 cut(s) 741
BtgI CCRYGG 1 cut(s) 212
BtgZI GCGATG 1 cut(s) 579
BtsCI GGATG 1 cut(s) 248
BtsIMutI CAGTG 1 cut(s) 632
BveI ACCTGC 1 cut(s) 703
Cac8I GCNNGC 2 cut(s) 354, 407
CfoI GCGC 2 cut(s) 718, 740
Cfr10I RCCGGY 2 cut(s) 352, 405
CseI GACGC 1 cut(s) 322
CspCI CAANNNNNGTGG 2 cut(s) 565, 600
CviAII CATG 6 cut(s) 49, 213, 239, 561, 649, 813
CviJI RGCY 3 cut(s) 352, 356, 686
CviKI_1 RGCY 3 cut(s) 352, 356, 686
DdeI CTNAG 2 cut(s) 309, 417
DpnI GATC 1 cut(s) 149
DpnII GATC 1 cut(s) 147
DrdI GACNNNNNNGTC 1 cut(s) 539
DseDI GACNNNNNNGTC 1 cut(s) 539
Eam1104I CTCTTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 102
Eco130I CCWWGG 2 cut(s) 133, 212
Eco32I GATATC 1 cut(s) 94
Eco57I CTGAAG 2 cut(s) 471, 720
Eco72I CACGTG 1 cut(s) 584
EcoRII CCWGG 1 cut(s) 733
EcoRV GATATC 1 cut(s) 94
EcoT14I CCWWGG 2 cut(s) 133, 212
ErhI CCWWGG 2 cut(s) 133, 212
Esp3I CGTCTC 1 cut(s) 312
FaeI CATG 6 cut(s) 52, 216, 242, 564, 652, 816
FalI AAGNNNNNCTT 2 cut(s) 217, 249
FaqI GGGAC 1 cut(s) 379
FatI CATG 6 cut(s) 48, 212, 238, 560, 648, 812
FblI GTMKAC 1 cut(s) 749
Fnu4HI GCNGC 2 cut(s) 265, 295
FokI GGATG 1 cut(s) 255
Fsp4HI GCNGC 2 cut(s) 265, 295
FspBI CTAG 1 cut(s) 820
GlaI GCGC 2 cut(s) 717, 739
GluI GCNGC 2 cut(s) 265, 295
HapII CCGG 5 cut(s) 353, 396, 406, 681, 754
HgaI GACGC 1 cut(s) 322
HhaI GCGC 2 cut(s) 718, 740
Hin1II CATG 6 cut(s) 52, 216, 242, 564, 652, 816
Hin6I GCGC 2 cut(s) 716, 738
HinP1I GCGC 2 cut(s) 716, 738
HinfI GANTC 5 cut(s) 191, 228, 301, 557, 804
HpaII CCGG 5 cut(s) 353, 396, 406, 681, 754
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 4 cut(s) 430, 587, 750, 785
Hpy188I TCNGA 6 cut(s) 98, 154, 312, 418, 474, 545
Hpy188III TCNNGA 3 cut(s) 195, 320, 431
Hpy8I GTNNAC 4 cut(s) 430, 587, 750, 785
Hpy99I CGWCG 1 cut(s) 804
HpyAV CCTTC 3 cut(s) 82, 155, 319
HpyCH4III ACNGT 2 cut(s) 130, 782
HpyCH4IV ACGT 3 cut(s) 534, 583, 633
HpyCH4V TGCA 8 cut(s) 80, 120, 246, 267, 347, 569, 576, 657
HpyF10VI GCNNNNNNNGC 3 cut(s) 291, 353, 491
HpyF3I CTNAG 2 cut(s) 309, 417
HpySE526I ACGT 3 cut(s) 534, 583, 633
Hsp92II CATG 6 cut(s) 52, 216, 242, 564, 652, 816
HspAI GCGC 2 cut(s) 716, 738
KroI GCCGGC 2 cut(s) 352, 405
KroNI GCCGGC 2 cut(s) 354, 407
Kzo9I GATC 1 cut(s) 147
LguI GCTCTTC 1 cut(s) 102
Lsp1109I GCAGC 2 cut(s) 251, 281
LweI GCATC 5 cut(s) 166, 233, 392, 556, 703
MaeI CTAG 1 cut(s) 820
MaeII ACGT 3 cut(s) 534, 583, 633
MaeIII GTNAC 2 cut(s) 629, 776
MalI GATC 1 cut(s) 149
MbiI CCGCTC 1 cut(s) 112
MboI GATC 1 cut(s) 147
MboII GAAGA 4 cut(s) 29, 119, 150, 722
MfeI CAATTG 1 cut(s) 590
MluCI AATT 3 cut(s) 81, 278, 590
MlyI GAGTC 1 cut(s) 798
MmeI TCCRAC 1 cut(s) 523
MnlI CCTC 1 cut(s) 41
MroNI GCCGGC 2 cut(s) 352, 405
MseI TTAA 1 cut(s) 333
MslI CAYNNNNRTG 1 cut(s) 435
MspI CCGG 5 cut(s) 353, 396, 406, 681, 754
MspR9I CCNGG 4 cut(s) 397, 682, 735, 754
MunI CAATTG 1 cut(s) 590
MvaI CCWGG 1 cut(s) 735
MvnI CGCG 1 cut(s) 740
MwoI GCNNNNNNNGC 3 cut(s) 291, 353, 491
NaeI GCCGGC 2 cut(s) 354, 407
NciI CCSGG 3 cut(s) 397, 682, 754
NcoI CCATGG 1 cut(s) 212
NdeII GATC 1 cut(s) 147
NgoMIV GCCGGC 2 cut(s) 352, 405
NlaIII CATG 6 cut(s) 52, 216, 242, 564, 652, 816
NmeAIII GCCGAG 1 cut(s) 744
NmuCI GTSAC 2 cut(s) 629, 776
PciSI GCTCTTC 1 cut(s) 102
PdiI GCCGGC 2 cut(s) 354, 407
PfeI GAWTC 4 cut(s) 191, 228, 301, 557
PkrI GCNGC 2 cut(s) 266, 296
PleI GAGTC 1 cut(s) 798
PmaCI CACGTG 1 cut(s) 584
PmlI CACGTG 1 cut(s) 584
PpsI GAGTC 1 cut(s) 798
Ppu21I YACGTR 1 cut(s) 584
Psp6I CCWGG 1 cut(s) 733
PspCI CACGTG 1 cut(s) 584
PspGI CCWGG 1 cut(s) 733
RseI CAYNNNNRTG 1 cut(s) 435
SapI GCTCTTC 1 cut(s) 102
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 2 cut(s) 265, 295
Sau3AI GATC 1 cut(s) 147
SchI GAGTC 1 cut(s) 798
ScrFI CCNGG 4 cut(s) 397, 682, 735, 754
SetI ASST 7 cut(s) 330, 339, 493, 537, 586, 636, 697
SfaNI GCATC 5 cut(s) 166, 233, 392, 556, 703
SmiMI CAYNNNNRTG 1 cut(s) 435
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 3 cut(s) 81, 278, 590
SsiI CCGC 1 cut(s) 112
SspMI CTAG 1 cut(s) 820
StyD4I CCNGG 4 cut(s) 395, 680, 733, 752
StyI CCWWGG 2 cut(s) 133, 212
TaaI ACNGT 2 cut(s) 130, 782
TaiI ACGT 3 cut(s) 537, 586, 636
TaqI TCGA 2 cut(s) 196, 708
TasI AATT 3 cut(s) 81, 278, 590
TfiI GAWTC 4 cut(s) 191, 228, 301, 557
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 1 cut(s) 632
TseFI GTSAC 2 cut(s) 629, 776
TseI GCWGC 2 cut(s) 264, 294
Tsp45I GTSAC 2 cut(s) 629, 776
TspDTI ATGAA 6 cut(s) 174, 291, 549, 617, 653, 665
TspGWI ACGGA 2 cut(s) 54, 294
TspRI CASTG 1 cut(s) 632
XapI RAATTY 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 749
XspI CTAG 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.