Rorug04G0074300

Uncharacterized conserved protein (DUF2054)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005830
Physical Location & Seq
Reverse (-)
11523223 .. 11524777
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0074300.1

Sequence Viewer

Length: 1089 bp
ATGTGTTGTCTAGAGCACAGGAGAGACTGGGTGGCTTATGTTGTCCACACCATCAAACTGTCGGATTTGTTATCATATTCGGCGGTGGGGGACGAGCCACCGCGATTACGGCAAGTGGCTTATAAGGCCGGGTCGCATCTCCCCGGCTCTGTGGGTTGCGGTGTTTGGGGATCCCAAATTGTTTTCACCGGCGGCGTGACACCCAGCTCCCCTTTCGGCTTCAGGACAATAGGTCCTGACTCAGTTTGGCATACAAATGTTTATGCCTTTGAAACACACACAAATGAGATCAGAAAGCTTGATGCCAATTTACAAGGAGGGAAACTGGAACCTTTGATGGTGGAGCTGGAGGGCAAACTGTACGCTCTTTCCTATAGGCTGGTCGGCCATCCTCCCTCATTTGAGGTGTTTGACCCTAAAATAGGCAAGTGGGAAGGTTTACCCCAACCTCCATTCTTCCAAGTTGACTCTCCATATCATGAACATGGTAATTTCTCTTATGCAATCGCCGGCACAAAGATGTTTGCCTCCCATGAAAAATGTCCAGTATTCTGCTTTGACGTCTCCCACCCGGATAGAGAATGGAGACTAGTCCCTACTATGTGCCGGGGTGGTCATTTTCCCTTCGATAACAGGGCTTTAGTCCTGGACCTGCAGGAGGACGACAAGAAGAAAATAATGTTTGCCTACCGTTATGAGCCTTGGCGTCTAAGGGTATATCTTATGTCATTGTCTGAAAACCAAGAAATTATCACAAAAATCTGTCATCTGAGGATGCCAGTGTTGCCTGGTGAATTGGGTTTGCCGCAACACTGTGAATGTTTGCACATAGGAGGTCAGAAAGCCTGCCTTGTTGTCACCGAATTCGACTTCCGTGGTGGAGTCGACGCTCCTGCTTATAATGAGCTCAATACTCATAAGAGACACGGTGCAGTCATACCATTTCAATTTGAAGTTGATATCACCAAGGTGGACAAGGACAAGAAAAATTGTTTAACACTCCAATTCATGCCTCTTCGCAGCTTCGAATACCATACCAACCCATCCAGTTTTCCTGAACCTGAAACGGTTGGCTGCTTTCTGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2054 Glyco_transf_18

Protein Analysis

362

Amino Acids

41.13

Weight (kDa)

6.38

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 66 - 211 1.6e-09 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 123, 900
AatII GACGTC 1 cut(s) 564
Acc36I ACCTGC 1 cut(s) 660
AccI GTMKAC 1 cut(s) 885
AccII CGCG 1 cut(s) 103
AciI CCGC 5 cut(s) 83, 101, 159, 192, 806
AclWI GGATC 2 cut(s) 165, 178
AcoI YGGCCR 1 cut(s) 385
AcsI RAATTY 1 cut(s) 863
AcuI CTGAAG 1 cut(s) 205
AcyI GRCGYC 2 cut(s) 561, 706
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 2 cut(s) 422, 658
AgsI TTSAA 3 cut(s) 272, 947, 953
AhdI GACNNNNNGTC 1 cut(s) 231
AhlI ACTAGT 1 cut(s) 589
AjnI CCWGG 2 cut(s) 645, 787
AleI CACNNNNGTG 1 cut(s) 968
AluBI AGCT 5 cut(s) 207, 298, 346, 907, 1023
AluI AGCT 5 cut(s) 207, 298, 346, 907, 1023
Alw21I GWGCWC 2 cut(s) 18, 909
Alw26I GTCTC 4 cut(s) 18, 568, 580, 916
AlwI GGATC 2 cut(s) 165, 178
AoxI GGCC 2 cut(s) 126, 385
ApeKI GCWGC 2 cut(s) 1020, 1074
ApoI RAATTY 1 cut(s) 863
AspS9I GGNCC 2 cut(s) 233, 649
AsuC2I CCSGG 4 cut(s) 130, 144, 572, 608
AsuHPI GGTGA 4 cut(s) 178, 803, 850, 955
AsuII TTCGAA 1 cut(s) 1026
AvaII GGWCC 2 cut(s) 233, 649
BamHI GGATCC 1 cut(s) 170
BanII GRGCYC 1 cut(s) 909
Bbv12I GWGCWC 2 cut(s) 18, 909
BbvI GCAGC 2 cut(s) 1032, 1061
BccI CCATC 4 cut(s) 59, 331, 396, 1051
BceAI ACGGC 1 cut(s) 125
BcgI CGANNNNNNTGC 2 cut(s) 875, 909
BciT130I CCWGG 2 cut(s) 647, 789
BcnI CCSGG 4 cut(s) 130, 144, 572, 608
BcoDI GTCTC 4 cut(s) 18, 568, 580, 916
BcuI ACTAGT 1 cut(s) 589
BfaI CTAG 2 cut(s) 11, 590
BfmI CTRYAG 2 cut(s) 373, 653
BfuAI ACCTGC 1 cut(s) 660
BisI GCNGC 4 cut(s) 193, 806, 1021, 1075
BlsI GCNGC 4 cut(s) 194, 807, 1022, 1076
Bme1390I CCNGG 6 cut(s) 130, 144, 572, 608, 647, 789
Bme18I GGWCC 2 cut(s) 233, 649
BmeRI GACNNNNNGTC 1 cut(s) 231
BmgT120I GGNCC 2 cut(s) 233, 649
BmiI GGNNCC 2 cut(s) 172, 330
BmrFI CCNGG 6 cut(s) 130, 144, 572, 608, 647, 789
BmrI ACTGGG 1 cut(s) 37
BmsI GCATC 3 cut(s) 145, 292, 765
BmuI ACTGGG 1 cut(s) 37
BpmI CTGGAG 1 cut(s) 368
Bpu14I TTCGAA 1 cut(s) 1026
BpuMI CCSGG 4 cut(s) 130, 144, 572, 608
BsaHI GRCGYC 2 cut(s) 561, 706
BsaJI CCNNGG 5 cut(s) 142, 607, 701, 874, 966
Bsc4I CCNNNNNNNGG 2 cut(s) 422, 658
Bse118I RCCGGY 2 cut(s) 188, 509
Bse1I ACTGG 5 cut(s) 32, 330, 545, 779, 1047
BseBI CCWGG 2 cut(s) 647, 789
BseDI CCNNGG 5 cut(s) 142, 607, 701, 874, 966
BseGI GGATG 3 cut(s) 388, 780, 1043
BseLI CCNNNNNNNGG 2 cut(s) 422, 658
BseMII CTCAG 2 cut(s) 255, 761
BseNI ACTGG 5 cut(s) 32, 330, 545, 779, 1047
BseXI GCAGC 2 cut(s) 1032, 1061
BseYI CCCAGC 1 cut(s) 203
BsgI GTGCAG 1 cut(s) 951
Bsh1236I CGCG 1 cut(s) 103
BshFI GGCC 2 cut(s) 128, 387
BsiHKAI GWGCWC 2 cut(s) 18, 909
BsiSI CCGG 6 cut(s) 129, 144, 189, 510, 572, 607
BslFI GGGAC 2 cut(s) 104, 578
BslI CCNNNNNNNGG 2 cut(s) 422, 658
BsmAI GTCTC 4 cut(s) 18, 568, 580, 916
BsmBI CGTCTC 1 cut(s) 568
BsmFI GGGAC 2 cut(s) 104, 578
BsnI GGCC 2 cut(s) 128, 387
Bsp119I TTCGAA 1 cut(s) 1026
Bsp1286I GDGCHC 2 cut(s) 18, 909
Bsp143I GATC 2 cut(s) 170, 288
BspACI CCGC 5 cut(s) 83, 101, 159, 192, 806
BspANI GGCC 2 cut(s) 128, 387
BspCNI CTCAG 2 cut(s) 254, 762
BspFNI CGCG 1 cut(s) 103
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 2 cut(s) 172, 330
BspMAI CTGCAG 1 cut(s) 657
BspMI ACCTGC 1 cut(s) 660
BspPI GGATC 2 cut(s) 165, 178
BspT104I TTCGAA 1 cut(s) 1026
BsrFI RCCGGY 2 cut(s) 188, 509
BsrI ACTGG 5 cut(s) 32, 330, 545, 779, 1047
BssAI RCCGGY 2 cut(s) 188, 509
BssECI CCNNGG 5 cut(s) 142, 607, 701, 874, 966
BssMI GATC 2 cut(s) 170, 288
BssNI GRCGYC 2 cut(s) 561, 706
BssT1I CCWWGG 2 cut(s) 701, 966
Bst2UI CCWGG 2 cut(s) 647, 789
Bst4CI ACNGT 6 cut(s) 60, 360, 692, 815, 929, 1069
Bst6I CTCTTC 1 cut(s) 1020
BstACI GRCGYC 2 cut(s) 561, 706
BstBI TTCGAA 1 cut(s) 1026
BstC8I GCNNGC 2 cut(s) 511, 847
BstDEI CTNAG 3 cut(s) 241, 710, 770
BstDSI CCRYGG 1 cut(s) 874
BstENI CCTNNNNNAGG 2 cut(s) 420, 656
BstF5I GGATG 3 cut(s) 388, 780, 1043
BstFNI CGCG 1 cut(s) 103
BstKTI GATC 2 cut(s) 173, 291
BstMAI GTCTC 4 cut(s) 18, 568, 580, 916
BstMBI GATC 2 cut(s) 170, 288
BstMWI GCNNNNNNNGC 3 cut(s) 109, 125, 784
BstNI CCWGG 2 cut(s) 647, 789
BstSCI CCNGG 6 cut(s) 128, 142, 570, 606, 645, 787
BstSFI CTRYAG 2 cut(s) 373, 653
BstUI CGCG 1 cut(s) 103
BstV1I GCAGC 2 cut(s) 1032, 1061
BstX2I RGATCY 1 cut(s) 170
BstYI RGATCY 1 cut(s) 170
BsuRI GGCC 2 cut(s) 128, 387
BtgI CCRYGG 1 cut(s) 874
BtsCI GGATG 3 cut(s) 388, 780, 1043
BtsIMutI CAGTG 2 cut(s) 786, 811
BveI ACCTGC 1 cut(s) 660
Cac8I GCNNGC 2 cut(s) 511, 847
CciI TCATGA 1 cut(s) 478
Cfr10I RCCGGY 2 cut(s) 188, 509
Cfr13I GGNCC 2 cut(s) 233, 649
CseI GACGC 2 cut(s) 695, 896
Csp6I GTAC 1 cut(s) 361
CviAII CATG 4 cut(s) 479, 485, 533, 1009
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 3 cut(s) 241, 710, 770
DpnI GATC 2 cut(s) 172, 290
DpnII GATC 2 cut(s) 170, 288
DriI GACNNNNNGTC 1 cut(s) 231
EaeI YGGCCR 1 cut(s) 385
Eam1104I CTCTTC 1 cut(s) 1020
Eam1105I GACNNNNNGTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 1020
Ecl136II GAGCTC 1 cut(s) 907
Eco130I CCWWGG 2 cut(s) 701, 966
Eco24I GRGCYC 1 cut(s) 909
Eco32I GATATC 1 cut(s) 961
Eco47I GGWCC 2 cut(s) 233, 649
Eco53kI GAGCTC 1 cut(s) 907
Eco57I CTGAAG 1 cut(s) 205
EcoICRI GAGCTC 1 cut(s) 907
EcoNI CCTNNNNNAGG 2 cut(s) 420, 656
EcoO109I RGGNCCY 1 cut(s) 233
EcoRI GAATTC 1 cut(s) 863
EcoRII CCWGG 2 cut(s) 645, 787
EcoRV GATATC 1 cut(s) 961
EcoT14I CCWWGG 2 cut(s) 701, 966
EcoT38I GRGCYC 1 cut(s) 909
ErhI CCWWGG 2 cut(s) 701, 966
Esp3I CGTCTC 1 cut(s) 568
FaeI CATG 4 cut(s) 482, 488, 536, 1012
FalI AAGNNNNNCTT 2 cut(s) 834, 866
FaqI GGGAC 2 cut(s) 104, 578
FatI CATG 4 cut(s) 478, 484, 532, 1008
FblI GTMKAC 1 cut(s) 885
Fnu4HI GCNGC 4 cut(s) 193, 806, 1021, 1075
FokI GGATG 3 cut(s) 375, 787, 1030
FriOI GRGCYC 1 cut(s) 909
Fsp4HI GCNGC 4 cut(s) 193, 806, 1021, 1075
FspBI CTAG 2 cut(s) 11, 590
GluI GCNGC 4 cut(s) 193, 806, 1021, 1075
GsaI CCCAGC 1 cut(s) 207
GsuI CTGGAG 1 cut(s) 368
HaeIII GGCC 2 cut(s) 128, 387
HapII CCGG 6 cut(s) 129, 144, 189, 510, 572, 607
HgaI GACGC 2 cut(s) 695, 896
Hin1I GRCGYC 2 cut(s) 561, 706
Hin1II CATG 4 cut(s) 482, 488, 536, 1012
HincII GTYRAC 2 cut(s) 466, 886
HindII GTYRAC 2 cut(s) 466, 886
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 3 cut(s) 239, 467, 882
HpaII CCGG 6 cut(s) 129, 144, 189, 510, 572, 607
HphI GGTGA 4 cut(s) 178, 803, 850, 955
Hpy166II GTNNAC 5 cut(s) 46, 440, 466, 886, 973
Hpy188I TCNGA 5 cut(s) 64, 293, 736, 771, 840
Hpy188III TCNNGA 5 cut(s) 11, 223, 236, 479, 1055
Hpy8I GTNNAC 5 cut(s) 46, 440, 466, 886, 973
Hpy99I CGWCG 1 cut(s) 890
HpyAV CCTTC 2 cut(s) 428, 634
HpyCH4III ACNGT 6 cut(s) 60, 360, 692, 815, 929, 1069
HpyCH4IV ACGT 1 cut(s) 561
HpyCH4V TGCA 4 cut(s) 503, 655, 826, 932
HpyF10VI GCNNNNNNNGC 3 cut(s) 109, 125, 784
HpyF3I CTNAG 3 cut(s) 241, 710, 770
HpySE526I ACGT 1 cut(s) 561
Hsp92I GRCGYC 2 cut(s) 561, 706
Hsp92II CATG 4 cut(s) 482, 488, 536, 1012
KroI GCCGGC 1 cut(s) 509
KroNI GCCGGC 1 cut(s) 511
Kzo9I GATC 2 cut(s) 170, 288
LmnI GCTCC 3 cut(s) 212, 343, 895
Lsp1109I GCAGC 2 cut(s) 1032, 1061
LweI GCATC 3 cut(s) 145, 292, 765
MaeI CTAG 2 cut(s) 11, 590
MaeII ACGT 1 cut(s) 561
MaeIII GTNAC 2 cut(s) 196, 856
MalI GATC 2 cut(s) 172, 290
MboI GATC 2 cut(s) 170, 288
MboII GAAGA 3 cut(s) 448, 682, 1007
MflI RGATCY 1 cut(s) 170
MhlI GDGCHC 2 cut(s) 18, 909
MluCI AATT 9 cut(s) 177, 307, 490, 747, 794, 863, 947, 988, 1004
MlyI GAGTC 3 cut(s) 233, 461, 891
MmeI TCCRAC 1 cut(s) 42
MroNI GCCGGC 1 cut(s) 509
MseI TTAA 1 cut(s) 995
MslI CAYNNNNRTG 5 cut(s) 255, 282, 483, 518, 968
MspI CCGG 6 cut(s) 129, 144, 189, 510, 572, 607
MspR9I CCNGG 6 cut(s) 130, 144, 572, 608, 647, 789
MvaI CCWGG 2 cut(s) 647, 789
MvnI CGCG 1 cut(s) 103
MwoI GCNNNNNNNGC 3 cut(s) 109, 125, 784
NaeI GCCGGC 1 cut(s) 511
NciI CCSGG 4 cut(s) 130, 144, 572, 608
NdeII GATC 2 cut(s) 170, 288
NgoMIV GCCGGC 1 cut(s) 509
NlaIII CATG 4 cut(s) 482, 488, 536, 1012
NlaIV GGNNCC 2 cut(s) 172, 330
NmuCI GTSAC 2 cut(s) 196, 856
NspV TTCGAA 1 cut(s) 1026
OliI CACNNNNGTG 1 cut(s) 968
PagI TCATGA 1 cut(s) 478
PdiI GCCGGC 1 cut(s) 511
PfoI TCCNGGA 1 cut(s) 645
PkrI GCNGC 4 cut(s) 194, 807, 1022, 1076
PleI GAGTC 3 cut(s) 233, 461, 890
PpsI GAGTC 3 cut(s) 233, 461, 890
PpuMI RGGWCCY 1 cut(s) 233
PsiI TTATAA 2 cut(s) 123, 900
Psp124BI GAGCTC 1 cut(s) 909
Psp5II RGGWCCY 1 cut(s) 233
Psp6I CCWGG 2 cut(s) 645, 787
PspFI CCCAGC 1 cut(s) 203
PspGI CCWGG 2 cut(s) 645, 787
PspN4I GGNNCC 2 cut(s) 172, 330
PspPI GGNCC 2 cut(s) 233, 649
PspPPI RGGWCCY 1 cut(s) 233
PstI CTGCAG 1 cut(s) 657
PsuI RGATCY 1 cut(s) 170
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
RseI CAYNNNNRTG 5 cut(s) 255, 282, 483, 518, 968
SacI GAGCTC 1 cut(s) 909
SalI GTCGAC 1 cut(s) 884
SaqAI TTAA 1 cut(s) 995
SatI GCNGC 4 cut(s) 193, 806, 1021, 1075
Sau3AI GATC 2 cut(s) 170, 288
Sau96I GGNCC 2 cut(s) 233, 649
SbfI CCTGCAGG 1 cut(s) 657
SchI GAGTC 3 cut(s) 233, 461, 891
ScrFI CCNGG 6 cut(s) 130, 144, 572, 608, 647, 789
SdaI CCTGCAGG 1 cut(s) 657
SduI GDGCHC 2 cut(s) 18, 909
SfaNI GCATC 3 cut(s) 145, 292, 765
SfcI CTRYAG 2 cut(s) 373, 653
SfuI TTCGAA 1 cut(s) 1026
SgrAI CRCCGGYG 1 cut(s) 188
SinI GGWCC 2 cut(s) 233, 649
SmiMI CAYNNNNRTG 5 cut(s) 255, 282, 483, 518, 968
SpeI ACTAGT 1 cut(s) 589
Sse8387I CCTGCAGG 1 cut(s) 657
Sse9I AATT 9 cut(s) 177, 307, 490, 747, 794, 863, 947, 988, 1004
SsiI CCGC 5 cut(s) 83, 101, 159, 192, 806
SspMI CTAG 2 cut(s) 11, 590
SstI GAGCTC 1 cut(s) 909
StyD4I CCNGG 6 cut(s) 128, 142, 570, 606, 645, 787
StyI CCWWGG 2 cut(s) 701, 966
TaaI ACNGT 6 cut(s) 60, 360, 692, 815, 929, 1069
TaiI ACGT 1 cut(s) 564
TaqI TCGA 4 cut(s) 627, 867, 885, 1026
TasI AATT 9 cut(s) 177, 307, 490, 747, 794, 863, 947, 988, 1004
TauI GCSGC 2 cut(s) 195, 808
Tru1I TTAA 1 cut(s) 995
Tru9I TTAA 1 cut(s) 995
TscAI CASTG 2 cut(s) 786, 818
TseFI GTSAC 2 cut(s) 196, 856
TseI GCWGC 2 cut(s) 1020, 1074
Tsp45I GTSAC 2 cut(s) 196, 856
TspDTI ATGAA 3 cut(s) 495, 549, 997
TspGWI ACGGA 1 cut(s) 863
TspRI CASTG 2 cut(s) 786, 818
VpaK11BI GGWCC 2 cut(s) 233, 649
XagI CCTNNNNNAGG 2 cut(s) 420, 656
XapI RAATTY 1 cut(s) 863
XbaI TCTAGA 1 cut(s) 10
XmiI GTMKAC 1 cut(s) 885
XspI CTAG 2 cut(s) 11, 590
ZraI GACGTC 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.