RchiOBHm_Chr4g0406831

Uncharacterized conserved protein (DUF2054)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005830
Physical Location & Seq
Reverse (-)
28758384 .. 28761601
3218 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37811

Sequence Viewer

Length: 951 bp
ATGAGAATGTCCATATCCCGATTAAGCCTGTACTGTTTGTTTCTGCACGTCTTCATCCTTGGGATCTCGGCTATCAGGAAGGATGTGGGCTTTCTTCAGAAGCAGTTTTGCAGAAGTACAGTCCAAGGAAGATACTTACTGTCTGATGATAATGGCCATGTCTGTGACGCACTGTCAGTAAATCCTCAGTCTCGCTGCTGCCCTGAAAAAGGAGACAAGTTCTCTTGTCATGGTTGCAATGTTGTCTCAGAGTGCTGTAATTCTTATGAGTATTGCGTTTCGTGTTGTCAAGATCCTTCAAGGATAATAAAGGAACAAGTACTGGAAGTGAAGATTGCCAAGCCAGCTTCTGCCGGGAAATATTCCAGTGTTTTTGATTTCTGTGCTGGAAGGTGCCGGCATAATTCTGAGAGTGTGTTGATCAATGGAAAGTCTGATTCTCTTATCCATTTCAGATTTGTTCTTTCTGAACAGTTATATTTAGTCACAATGCAGGAAATTCAGTGCTTTAATGTAATTTGTGACCAATCACTTCTCTTAAACTTCAGGAGCTTAATGCTTTATTCGTTTATGCTGTCATATTCAGAAAAGATAGGGAGAACCACATCATTATGTGCAAGGTCGTATGGGGAGAGTGTTGCTCATGTTTTCTGCATTGTGAAGTTGGTTAGGGAGGTTTCGGGCATCTTTATGACCCTTTGTTCCTGTGGAATTCAAAGACTACTCTCTGTCTTCTATTGTTTTAGAGTGAAAGCATTTGTTCACGAAAATGCTTACCGTAGTGATTTCCATCACTGTTTTTCTCTGCCTTCAAATTCTTCAGATGTTAAGCTCATGCTTGAAATTATGATATTGCTTGTACTGTTGTACAGTTCCCATCTAAAATTTTTAGATGTTTTGGATATGCTTAATTTTCCTCCTCACTGCAATAGTAGAGAAATTTGTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2054 Glyco_transf_18

Protein Analysis

316

Amino Acids

35.93

Weight (kDa)

8.25

Isoelectric Point (pI)

39.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPRING1 PF10218 33 - 141 9.7e-30 SREBP regulating gene protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 393
AclWI GGATC 2 cut(s) 71, 287
AcoI YGGCCR 1 cut(s) 154
AcsI RAATTY 5 cut(s) 498, 711, 814, 884, 939
AcuI CTGAAG 3 cut(s) 80, 529, 804
AfaI GTAC 5 cut(s) 32, 118, 321, 861, 869
AfiI CCNNNNNNNGG 1 cut(s) 209
AgsI TTSAA 4 cut(s) 300, 716, 813, 842
AhdI GACNNNNNGTC 1 cut(s) 172
AjiI CACGTC 1 cut(s) 49
AluBI AGCT 3 cut(s) 347, 552, 832
AluI AGCT 3 cut(s) 347, 552, 832
Alw26I GTCTC 3 cut(s) 195, 207, 250
AlwI GGATC 2 cut(s) 71, 287
AlwNI CAGNNNCTG 1 cut(s) 350
AoxI GGCC 1 cut(s) 154
ApeKI GCWGC 2 cut(s) 195, 198
ApoI RAATTY 5 cut(s) 498, 711, 814, 884, 939
AsuC2I CCSGG 1 cut(s) 355
BalI TGGCCA 1 cut(s) 156
BanI GGYRCC 1 cut(s) 393
BbsI GAAGAC 2 cut(s) 43, 724
BbvI GCAGC 2 cut(s) 182, 185
BccI CCATC 2 cut(s) 798, 885
BclI TGATCA 1 cut(s) 420
BcnI CCSGG 1 cut(s) 355
BcoDI GTCTC 3 cut(s) 195, 207, 250
BisI GCNGC 2 cut(s) 196, 199
BlsI GCNGC 2 cut(s) 197, 200
BmcAI AGTACT 1 cut(s) 321
Bme1390I CCNGG 1 cut(s) 355
BmeRI GACNNNNNGTC 1 cut(s) 172
BmgBI CACGTC 1 cut(s) 49
BmiI GGNNCC 1 cut(s) 395
BmrFI CCNGG 1 cut(s) 355
BmsI GCATC 1 cut(s) 693
BpiI GAAGAC 2 cut(s) 43, 724
BplI GAGNNNNNCTC 2 cut(s) 625, 657
BpuMI CCSGG 1 cut(s) 355
BsaBI GATNNNNATC 1 cut(s) 789
BsaJI CCNNGG 2 cut(s) 58, 124
Bsc4I CCNNNNNNNGG 1 cut(s) 209
Bse118I RCCGGY 1 cut(s) 396
Bse1I ACTGG 2 cut(s) 327, 366
Bse3DI GCAATG 1 cut(s) 244
Bse8I GATNNNNATC 1 cut(s) 789
BseDI CCNNGG 2 cut(s) 58, 124
BseGI GGATG 2 cut(s) 54, 88
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 1 cut(s) 209
BseMI GCAATG 1 cut(s) 244
BseMII CTCAG 3 cut(s) 200, 261, 399
BseNI ACTGG 2 cut(s) 327, 366
BseRI GAGGAG 1 cut(s) 909
BseXI GCAGC 2 cut(s) 182, 185
BsgI GTGCAG 1 cut(s) 29
BshFI GGCC 1 cut(s) 156
BshNI GGYRCC 1 cut(s) 393
BsiSI CCGG 2 cut(s) 354, 397
BslI CCNNNNNNNGG 1 cut(s) 209
BsmAI GTCTC 3 cut(s) 195, 207, 250
BsnI GGCC 1 cut(s) 156
Bsp1407I TGTACA 1 cut(s) 867
Bsp143I GATC 3 cut(s) 63, 292, 420
BspANI GGCC 1 cut(s) 156
BspCNI CTCAG 3 cut(s) 199, 260, 400
BspLI GGNNCC 1 cut(s) 395
BspPI GGATC 2 cut(s) 71, 287
BspT107I GGYRCC 1 cut(s) 393
BsrDI GCAATG 1 cut(s) 244
BsrFI RCCGGY 1 cut(s) 396
BsrGI TGTACA 1 cut(s) 867
BsrI ACTGG 2 cut(s) 327, 366
BssAI RCCGGY 1 cut(s) 396
BssECI CCNNGG 2 cut(s) 58, 124
BssMI GATC 3 cut(s) 63, 292, 420
BssT1I CCWWGG 2 cut(s) 58, 124
Bst4CI ACNGT 9 cut(s) 35, 121, 141, 174, 474, 779, 797, 864, 872
BstAUI TGTACA 1 cut(s) 867
BstC8I GCNNGC 2 cut(s) 345, 398
BstDEI CTNAG 3 cut(s) 186, 247, 408
BstENI CCTNNNNNAGG 1 cut(s) 207
BstF5I GGATG 2 cut(s) 54, 88
BstKTI GATC 3 cut(s) 66, 295, 423
BstMAI GTCTC 3 cut(s) 195, 207, 250
BstMBI GATC 3 cut(s) 63, 292, 420
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 353
BstV1I GCAGC 2 cut(s) 182, 185
BstV2I GAAGAC 2 cut(s) 43, 724
BstX2I RGATCY 2 cut(s) 63, 292
BstYI RGATCY 2 cut(s) 63, 292
BsuRI GGCC 1 cut(s) 156
BtrI CACGTC 1 cut(s) 49
BtsCI GGATG 2 cut(s) 54, 88
BtsI GCAGTG 1 cut(s) 922
BtsIMutI CAGTG 5 cut(s) 170, 373, 509, 793, 922
Cac8I GCNNGC 2 cut(s) 345, 398
CaiI CAGNNNCTG 1 cut(s) 350
Cfr10I RCCGGY 1 cut(s) 396
CseI GACGC 1 cut(s) 176
Csp6I GTAC 5 cut(s) 31, 117, 320, 860, 868
CviAII CATG 4 cut(s) 158, 230, 644, 835
CviJI RGCY 8 cut(s) 27, 71, 90, 156, 343, 347, 552, 832
CviKI_1 RGCY 8 cut(s) 27, 71, 90, 156, 343, 347, 552, 832
CviQI GTAC 5 cut(s) 31, 117, 320, 860, 868
DdeI CTNAG 3 cut(s) 186, 247, 408
DpnI GATC 3 cut(s) 65, 294, 422
DpnII GATC 3 cut(s) 63, 292, 420
DriI GACNNNNNGTC 1 cut(s) 172
EaeI YGGCCR 1 cut(s) 154
Eam1105I GACNNNNNGTC 1 cut(s) 172
Eco130I CCWWGG 2 cut(s) 58, 124
Eco57I CTGAAG 3 cut(s) 80, 529, 804
EcoNI CCTNNNNNAGG 1 cut(s) 207
EcoRI GAATTC 1 cut(s) 711
EcoT14I CCWWGG 2 cut(s) 58, 124
ErhI CCWWGG 2 cut(s) 58, 124
FaeI CATG 4 cut(s) 161, 233, 647, 838
FatI CATG 4 cut(s) 157, 229, 643, 834
FbaI TGATCA 1 cut(s) 420
Fnu4HI GCNGC 2 cut(s) 196, 199
FokI GGATG 2 cut(s) 41, 95
Fsp4HI GCNGC 2 cut(s) 196, 199
GluI GCNGC 2 cut(s) 196, 199
HaeIII GGCC 1 cut(s) 156
HapII CCGG 2 cut(s) 354, 397
HgaI GACGC 1 cut(s) 176
Hin1II CATG 4 cut(s) 161, 233, 647, 838
HinfI GANTC 1 cut(s) 437
HpaII CCGG 2 cut(s) 354, 397
Hpy166II GTNNAC 1 cut(s) 763
Hpy188I TCNGA 9 cut(s) 99, 145, 250, 409, 436, 455, 469, 586, 823
Hpy188III TCNNGA 5 cut(s) 18, 76, 290, 547, 764
Hpy8I GTNNAC 1 cut(s) 763
HpyAV CCTTC 4 cut(s) 73, 306, 384, 819
HpyCH4III ACNGT 9 cut(s) 35, 121, 141, 174, 474, 779, 797, 864, 872
HpyCH4IV ACGT 1 cut(s) 48
HpyCH4V TGCA 7 cut(s) 46, 111, 237, 493, 617, 654, 927
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpyF3I CTNAG 3 cut(s) 186, 247, 408
HpySE526I ACGT 1 cut(s) 48
Hsp92II CATG 4 cut(s) 161, 233, 647, 838
KroI GCCGGC 1 cut(s) 396
KroNI GCCGGC 1 cut(s) 398
Ksp22I TGATCA 1 cut(s) 420
Kzo9I GATC 3 cut(s) 63, 292, 420
LmnI GCTCC 1 cut(s) 549
Lsp1109I GCAGC 2 cut(s) 182, 185
LweI GCATC 1 cut(s) 693
MaeII ACGT 1 cut(s) 48
MaeIII GTNAC 3 cut(s) 164, 484, 521
MalI GATC 3 cut(s) 65, 294, 422
MboI GATC 3 cut(s) 63, 292, 420
MboII GAAGA 6 cut(s) 43, 86, 141, 343, 724, 810
MflI RGATCY 2 cut(s) 63, 292
MlsI TGGCCA 1 cut(s) 156
MluNI TGGCCA 1 cut(s) 156
MnlI CCTC 4 cut(s) 195, 667, 927, 930
Mox20I TGGCCA 1 cut(s) 156
MroNI GCCGGC 1 cut(s) 396
MscI TGGCCA 1 cut(s) 156
MseI TTAA 7 cut(s) 23, 510, 539, 554, 828, 909, 949
MslI CAYNNNNRTG 4 cut(s) 162, 610, 689, 768
Msp20I TGGCCA 1 cut(s) 156
MspI CCGG 2 cut(s) 354, 397
MspR9I CCNGG 1 cut(s) 355
MwoI GCNNNNNNNGC 1 cut(s) 344
NaeI GCCGGC 1 cut(s) 398
NciI CCSGG 1 cut(s) 355
NdeII GATC 3 cut(s) 63, 292, 420
NgoMIV GCCGGC 1 cut(s) 396
NlaIII CATG 4 cut(s) 161, 233, 647, 838
NlaIV GGNNCC 1 cut(s) 395
NmeAIII GCCGAG 1 cut(s) 47
NmuCI GTSAC 3 cut(s) 164, 484, 521
PdiI GCCGGC 1 cut(s) 398
PfeI GAWTC 1 cut(s) 437
PkrI GCNGC 2 cut(s) 197, 200
PspN4I GGNNCC 1 cut(s) 395
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 2 cut(s) 63, 292
RsaI GTAC 5 cut(s) 32, 118, 321, 861, 869
RsaNI GTAC 5 cut(s) 31, 117, 320, 860, 868
RseI CAYNNNNRTG 4 cut(s) 162, 610, 689, 768
SaqAI TTAA 7 cut(s) 23, 510, 539, 554, 828, 909, 949
SatI GCNGC 2 cut(s) 196, 199
Sau3AI GATC 3 cut(s) 63, 292, 420
ScaI AGTACT 1 cut(s) 321
ScrFI CCNGG 1 cut(s) 355
SetI ASST 7 cut(s) 51, 349, 395, 554, 623, 678, 834
SfaNI GCATC 1 cut(s) 693
SmiMI CAYNNNNRTG 4 cut(s) 162, 610, 689, 768
SspI AATATT 1 cut(s) 362
StyD4I CCNGG 1 cut(s) 353
StyI CCWWGG 2 cut(s) 58, 124
TaaI ACNGT 9 cut(s) 35, 121, 141, 174, 474, 779, 797, 864, 872
TaiI ACGT 1 cut(s) 51
TatI WGTACW 5 cut(s) 30, 116, 319, 859, 867
TfiI GAWTC 1 cut(s) 437
Tru1I TTAA 7 cut(s) 23, 510, 539, 554, 828, 909, 949
Tru9I TTAA 7 cut(s) 23, 510, 539, 554, 828, 909, 949
TscAI CASTG 5 cut(s) 177, 373, 509, 800, 929
TseFI GTSAC 3 cut(s) 164, 484, 521
TseI GCWGC 2 cut(s) 195, 198
Tsp45I GTSAC 3 cut(s) 164, 484, 521
TspDTI ATGAA 1 cut(s) 43
TspRI CASTG 5 cut(s) 177, 373, 509, 800, 929
XagI CCTNNNNNAGG 1 cut(s) 207
XapI RAATTY 5 cut(s) 498, 711, 814, 884, 939
ZrmI AGTACT 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.