Rh4BG137600

Uncharacterized conserved protein (DUF2054)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0005830
Physical Location & Seq
Reverse (-)
24536712 .. 24544010
7299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG137600.1

Sequence Viewer

Length: 774 bp
ATGAGAATGTCCACATCCCGATTAAGCCTATACTGTTTGTTTCTGCACGTCTTCATCCTTGGGATCTCGGCTATCAGGAAGGATGTGGGCTTTCTTCAGAAGCAGTTTTGCAGAAATACAGTCCAAGGAAGATACTTACTGTCTGATGATAATGGCCATGTCTGTGACGCGCTGTCAGTAAATCCTCAGTCTCGCTGCTGCCCTGAAAAAGGAGACAAGTTCTCTTGTCATGGTTGCAACGTTGTCTCAGAGTGCTGTAATTCTTATGAGTATTGCGTTTCGTGTTGTCTAGATCCTTCAAGGATAGTAAAGGAACAAGTACTGGAAGTGAAGATTGCCAAGCCAGCTTCTGCAGGGAAATATTCCAGTGTTTTTGATTTCTGTGCTGGAAGGTGCCGGCATAATTCTGAGAGTGTGGTTCACGAAAATGCTTACCTTAGTGATTTCCATCACTGTTTTTCTCTGCCTTCAAATTCTTCAGCTGCAAATTATACGTTTTTAGAAGCCAGACTGAATGGCATTAATGTTCTTGTTGGAAGGCGAGGTGAATCCTGCGACTTGGTTTGCAAGTCAAAGGGACAGTCATGTGTTCCAAATAAGCTTTTGGTACTTAATCAGTGTGACATTATCCAGAGATATATGAGCTGCAAGGGAACTTGCCTGGCCAGTATTGGATCAGATCAACCTGCCGAAGTTGTCGATGACGCTCCTGAACATTTGAATCCTGGAGCATGCTTATCATTATCAGAATTTCCACCGATGTCCCAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2054 Glyco_transf_18

Protein Analysis

257

Amino Acids

28.24

Weight (kDa)

7.0

Isoelectric Point (pI)

52.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPRING1 PF10218 33 - 153 1e-36 SREBP regulating gene protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 694
AccB1I GGYRCC 1 cut(s) 393
AccII CGCG 1 cut(s) 170
AclI AACGTT 1 cut(s) 240
AclWI GGATC 3 cut(s) 71, 287, 682
AcoI YGGCCR 2 cut(s) 154, 663
AcsI RAATTY 2 cut(s) 472, 749
AcuI CTGAAG 2 cut(s) 80, 462
AfaI GTAC 2 cut(s) 321, 609
AfiI CCNNNNNNNGG 1 cut(s) 209
AgsI TTSAA 3 cut(s) 300, 471, 721
AhdI GACNNNNNGTC 1 cut(s) 172
AjiI CACGTC 1 cut(s) 49
AjnI CCWGG 3 cut(s) 660, 724, 765
AluBI AGCT 4 cut(s) 347, 482, 601, 645
AluI AGCT 4 cut(s) 347, 482, 601, 645
Alw26I GTCTC 3 cut(s) 195, 207, 250
AlwI GGATC 3 cut(s) 71, 287, 682
AlwNI CAGNNNCTG 1 cut(s) 350
AoxI GGCC 2 cut(s) 154, 663
ApeKI GCWGC 4 cut(s) 195, 198, 482, 645
ApoI RAATTY 2 cut(s) 472, 749
ArsI GACNNNNNNTTYG 2 cut(s) 566, 598
AseI ATTAAT 1 cut(s) 522
AspLEI GCGC 1 cut(s) 172
AsuHPI GGTGA 1 cut(s) 557
BalI TGGCCA 2 cut(s) 156, 665
BanI GGYRCC 1 cut(s) 393
BbsI GAAGAC 1 cut(s) 43
BbvI GCAGC 4 cut(s) 182, 185, 469, 632
BccI CCATC 1 cut(s) 456
BciT130I CCWGG 3 cut(s) 662, 726, 767
BcoDI GTCTC 3 cut(s) 195, 207, 250
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 351
BfuAI ACCTGC 1 cut(s) 694
BisI GCNGC 4 cut(s) 196, 199, 483, 646
BlsI GCNGC 4 cut(s) 197, 200, 484, 647
BmcAI AGTACT 1 cut(s) 321
Bme1390I CCNGG 3 cut(s) 662, 726, 767
BmeRI GACNNNNNGTC 1 cut(s) 172
BmgBI CACGTC 1 cut(s) 49
BmiI GGNNCC 1 cut(s) 395
BmrFI CCNGG 3 cut(s) 662, 726, 767
BpiI GAAGAC 1 cut(s) 43
BpmI CTGGAG 1 cut(s) 747
BsaBI GATNNNNATC 1 cut(s) 447
BsaJI CCNNGG 3 cut(s) 58, 124, 765
Bsc4I CCNNNNNNNGG 1 cut(s) 209
Bse118I RCCGGY 1 cut(s) 396
Bse1I ACTGG 3 cut(s) 327, 366, 666
Bse8I GATNNNNATC 1 cut(s) 447
BseBI CCWGG 3 cut(s) 662, 726, 767
BseDI CCNNGG 3 cut(s) 58, 124, 765
BseGI GGATG 3 cut(s) 14, 54, 88
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 1 cut(s) 209
BseMII CTCAG 3 cut(s) 200, 261, 399
BseNI ACTGG 3 cut(s) 327, 366, 666
BseXI GCAGC 4 cut(s) 182, 185, 469, 632
BsgI GTGCAG 1 cut(s) 29
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 2 cut(s) 156, 665
BshNI GGYRCC 1 cut(s) 393
BsiSI CCGG 1 cut(s) 397
BslFI GGGAC 2 cut(s) 591, 748
BslI CCNNNNNNNGG 1 cut(s) 209
BsmAI GTCTC 3 cut(s) 195, 207, 250
BsmFI GGGAC 2 cut(s) 591, 748
BsnI GGCC 2 cut(s) 156, 665
Bsp143I GATC 4 cut(s) 63, 292, 674, 679
BspANI GGCC 2 cut(s) 156, 665
BspCNI CTCAG 3 cut(s) 199, 260, 400
BspFNI CGCG 1 cut(s) 170
BspLI GGNNCC 1 cut(s) 395
BspMAI CTGCAG 1 cut(s) 355
BspMI ACCTGC 1 cut(s) 694
BspPI GGATC 3 cut(s) 71, 287, 682
BspT107I GGYRCC 1 cut(s) 393
BsrFI RCCGGY 1 cut(s) 396
BsrI ACTGG 3 cut(s) 327, 366, 666
BssAI RCCGGY 1 cut(s) 396
BssECI CCNNGG 3 cut(s) 58, 124, 765
BssMI GATC 4 cut(s) 63, 292, 674, 679
BssT1I CCWWGG 2 cut(s) 58, 124
Bst2UI CCWGG 3 cut(s) 662, 726, 767
Bst4CI ACNGT 5 cut(s) 35, 121, 141, 455, 582
BstC8I GCNNGC 3 cut(s) 345, 398, 733
BstDEI CTNAG 4 cut(s) 186, 247, 408, 437
BstENI CCTNNNNNAGG 1 cut(s) 207
BstF5I GGATG 3 cut(s) 14, 54, 88
BstFNI CGCG 1 cut(s) 170
BstHHI GCGC 1 cut(s) 172
BstKTI GATC 4 cut(s) 66, 295, 677, 682
BstMAI GTCTC 3 cut(s) 195, 207, 250
BstMBI GATC 4 cut(s) 63, 292, 674, 679
BstMWI GCNNNNNNNGC 1 cut(s) 344
BstNI CCWGG 3 cut(s) 662, 726, 767
BstNSI RCATGY 1 cut(s) 735
BstSCI CCNGG 3 cut(s) 660, 724, 765
BstSFI CTRYAG 1 cut(s) 351
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 4 cut(s) 182, 185, 469, 632
BstV2I GAAGAC 1 cut(s) 43
BstX2I RGATCY 2 cut(s) 63, 292
BstYI RGATCY 2 cut(s) 63, 292
BsuRI GGCC 2 cut(s) 156, 665
BtrI CACGTC 1 cut(s) 49
BtsCI GGATG 3 cut(s) 14, 54, 88
BtsIMutI CAGTG 3 cut(s) 373, 451, 623
BveI ACCTGC 1 cut(s) 694
Cac8I GCNNGC 3 cut(s) 345, 398, 733
CaiI CAGNNNCTG 1 cut(s) 350
CfoI GCGC 1 cut(s) 172
Cfr10I RCCGGY 1 cut(s) 396
CseI GACGC 2 cut(s) 176, 713
Csp6I GTAC 2 cut(s) 320, 608
CviAII CATG 5 cut(s) 158, 230, 585, 732, 771
CviQI GTAC 2 cut(s) 320, 608
DdeI CTNAG 4 cut(s) 186, 247, 408, 437
DpnI GATC 4 cut(s) 65, 294, 676, 681
DpnII GATC 4 cut(s) 63, 292, 674, 679
DriI GACNNNNNGTC 1 cut(s) 172
EaeI YGGCCR 2 cut(s) 154, 663
Eam1105I GACNNNNNGTC 1 cut(s) 172
Eco130I CCWWGG 2 cut(s) 58, 124
Eco57I CTGAAG 2 cut(s) 80, 462
EcoNI CCTNNNNNAGG 1 cut(s) 207
EcoRII CCWGG 3 cut(s) 660, 724, 765
EcoT14I CCWWGG 2 cut(s) 58, 124
ErhI CCWWGG 2 cut(s) 58, 124
FaeI CATG 5 cut(s) 161, 233, 588, 735, 774
FaqI GGGAC 2 cut(s) 591, 748
FatI CATG 5 cut(s) 157, 229, 584, 731, 770
Fnu4HI GCNGC 4 cut(s) 196, 199, 483, 646
FokI GGATG 2 cut(s) 41, 95
Fsp4HI GCNGC 4 cut(s) 196, 199, 483, 646
FspBI CTAG 1 cut(s) 290
GlaI GCGC 1 cut(s) 171
GluI GCNGC 4 cut(s) 196, 199, 483, 646
GsuI CTGGAG 1 cut(s) 747
HaeIII GGCC 2 cut(s) 156, 665
HapII CCGG 1 cut(s) 397
HgaI GACGC 2 cut(s) 176, 713
HhaI GCGC 1 cut(s) 172
Hin1II CATG 5 cut(s) 161, 233, 588, 735, 774
Hin6I GCGC 1 cut(s) 170
HinP1I GCGC 1 cut(s) 170
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 2 cut(s) 548, 721
HpaII CCGG 1 cut(s) 397
HphI GGTGA 1 cut(s) 557
Hpy166II GTNNAC 2 cut(s) 12, 421
Hpy188I TCNGA 6 cut(s) 99, 145, 250, 409, 679, 748
Hpy188III TCNNGA 6 cut(s) 18, 76, 290, 422, 631, 710
Hpy8I GTNNAC 2 cut(s) 12, 421
HpyAV CCTTC 5 cut(s) 73, 306, 384, 477, 531
HpyCH4III ACNGT 5 cut(s) 35, 121, 141, 455, 582
HpyCH4IV ACGT 3 cut(s) 48, 240, 494
HpyCH4V TGCA 7 cut(s) 46, 111, 237, 353, 485, 567, 648
HpyF10VI GCNNNNNNNGC 1 cut(s) 344
HpyF3I CTNAG 4 cut(s) 186, 247, 408, 437
HpySE526I ACGT 3 cut(s) 48, 240, 494
Hsp92II CATG 5 cut(s) 161, 233, 588, 735, 774
HspAI GCGC 1 cut(s) 170
KroI GCCGGC 1 cut(s) 396
KroNI GCCGGC 1 cut(s) 398
Kzo9I GATC 4 cut(s) 63, 292, 674, 679
LmnI GCTCC 2 cut(s) 712, 728
Lsp1109I GCAGC 4 cut(s) 182, 185, 469, 632
MaeI CTAG 1 cut(s) 290
MaeII ACGT 3 cut(s) 48, 240, 494
MaeIII GTNAC 2 cut(s) 164, 620
MalI GATC 4 cut(s) 65, 294, 676, 681
MboI GATC 4 cut(s) 63, 292, 674, 679
MboII GAAGA 5 cut(s) 43, 86, 141, 343, 468
MflI RGATCY 2 cut(s) 63, 292
MlsI TGGCCA 2 cut(s) 156, 665
MluCI AATT 5 cut(s) 259, 403, 472, 487, 749
MluNI TGGCCA 2 cut(s) 156, 665
MmeI TCCRAC 1 cut(s) 514
MnlI CCTC 2 cut(s) 195, 536
Mox20I TGGCCA 2 cut(s) 156, 665
MroNI GCCGGC 1 cut(s) 396
MscI TGGCCA 2 cut(s) 156, 665
MseI TTAA 3 cut(s) 23, 522, 612
MslI CAYNNNNRTG 2 cut(s) 162, 426
Msp20I TGGCCA 2 cut(s) 156, 665
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 1 cut(s) 397
MspR9I CCNGG 3 cut(s) 662, 726, 767
MvaI CCWGG 3 cut(s) 662, 726, 767
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 1 cut(s) 344
NaeI GCCGGC 1 cut(s) 398
NdeII GATC 4 cut(s) 63, 292, 674, 679
NgoMIV GCCGGC 1 cut(s) 396
NlaIII CATG 5 cut(s) 161, 233, 588, 735, 774
NlaIV GGNNCC 1 cut(s) 395
NmeAIII GCCGAG 1 cut(s) 47
NmuCI GTSAC 2 cut(s) 164, 620
NspI RCATGY 1 cut(s) 735
PaeI GCATGC 1 cut(s) 735
PdiI GCCGGC 1 cut(s) 398
PfeI GAWTC 2 cut(s) 548, 721
PfoI TCCNGGA 1 cut(s) 724
PkrI GCNGC 4 cut(s) 197, 200, 484, 647
PshBI ATTAAT 1 cut(s) 522
Psp1406I AACGTT 1 cut(s) 240
Psp6I CCWGG 3 cut(s) 660, 724, 765
PspGI CCWGG 3 cut(s) 660, 724, 765
PspN4I GGNNCC 1 cut(s) 395
PstI CTGCAG 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 2 cut(s) 63, 292
PvuII CAGCTG 1 cut(s) 482
RsaI GTAC 2 cut(s) 321, 609
RsaNI GTAC 2 cut(s) 320, 608
RseI CAYNNNNRTG 2 cut(s) 162, 426
SaqAI TTAA 3 cut(s) 23, 522, 612
SatI GCNGC 4 cut(s) 196, 199, 483, 646
Sau3AI GATC 4 cut(s) 63, 292, 674, 679
ScaI AGTACT 1 cut(s) 321
ScrFI CCNGG 3 cut(s) 662, 726, 767
SfcI CTRYAG 1 cut(s) 351
SmiMI CAYNNNNRTG 2 cut(s) 162, 426
SphI GCATGC 1 cut(s) 735
Sse9I AATT 5 cut(s) 259, 403, 472, 487, 749
SspI AATATT 1 cut(s) 362
SspMI CTAG 1 cut(s) 290
StyD4I CCNGG 3 cut(s) 660, 724, 765
StyI CCWWGG 2 cut(s) 58, 124
TaaI ACNGT 5 cut(s) 35, 121, 141, 455, 582
TaiI ACGT 3 cut(s) 51, 243, 497
TaqI TCGA 1 cut(s) 699
TasI AATT 5 cut(s) 259, 403, 472, 487, 749
TatI WGTACW 1 cut(s) 319
TfiI GAWTC 2 cut(s) 548, 721
Tru1I TTAA 3 cut(s) 23, 522, 612
Tru9I TTAA 3 cut(s) 23, 522, 612
TscAI CASTG 3 cut(s) 373, 458, 623
TseFI GTSAC 2 cut(s) 164, 620
TseI GCWGC 4 cut(s) 195, 198, 482, 645
Tsp45I GTSAC 2 cut(s) 164, 620
TspDTI ATGAA 1 cut(s) 43
TspRI CASTG 3 cut(s) 373, 458, 623
VspI ATTAAT 1 cut(s) 522
XagI CCTNNNNNAGG 1 cut(s) 207
XapI RAATTY 2 cut(s) 472, 749
XbaI TCTAGA 1 cut(s) 289
XceI RCATGY 1 cut(s) 735
XspI CTAG 1 cut(s) 290
ZrmI AGTACT 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.