AT4G08540

Vacuolar sorting 38 and autophagy-related subunit 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009921
Physical Location & Seq
Forward (+)
5433621 .. 5436524
2904 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G08540.1

Sequence Viewer

Length: 1422 bp
ATGACAAAACGATCCAGTAACTGCGCTATTTGCGACAACACTAATCGTCCTTGTATCTGTACTGCTTGCGTTAATCATAGATTGATTGAGTATAACACTTTGTTGAAGTCACTCAAAACTCGAAGAGATTCATTATTATCAAGATTCAATGAGCTATTGGAATCTAAGGGTAAAGCTGATGATCAGAAGAATTGGAGATTGATTCAAAACGAGAAGATTTCGAAGTTGAAGAAGAAGCTTAAGAGTAATAAGGAGTTAGTTACTCAAGGGAAGGTTAAGATTGAGAGAGGGTCTTCTGATCTTAAAGTTAAATATGGAGTTCTTGATTCAGCTCGTTCTACTCTTGAGAAAACTAGAGTTGAACAAGTGGAGAAGTATTTTCCTAATTTGATATGTACTCAGAGCCTTGGTCATATGGCGATTTCGTCTGAGCGTCTTCATAAGCAGTCAGTTGTTGTGAAACAGATATGCAAATTGTTTCCTTTACGTCGAGTTAGTTTTGATGGAGAGAGTCAGAACGGTTCAGTTCGTCAATATGATGTAATTTGCAATTCACGTCTTCCGAGTGGTTTGGATCCTCATTCTATCCCATCAGAAGAGCTTGCTGTCTCGTTGGGGTATATGGTCCAGCTTCTGAATCTTGTTGTTCATAACTTAGCCGCTCCTGCACTACATAGCTCGGGTTTTGCGGGCTCTTGCTCTCGTATTTGGCAACGAGATTCATATTGGGACGGACGTACTTCTACTCGAAGCAATGAGTATCCTCTCTTCATACCTCGCCGGAATTATTGTTCAACCAGCGTTGAAAACTCATGGACAGATAAAAACTCCAGCAATTTCGGTGTTGCTTCCATGGAGTCCGATAGAAAAGAACCCCGTCTGGACTCTCCAGGAAGCAACAGCTTTAAGTACTCCTCTGCCTCTCCTCATTCGATTGAGTCACACAGAGATCTACAGAAAGGGATAGCTCTTCTCAAGAAAAGTGTTGCGTGCTTGACTGCGTATTGTTACAATTCACTTTGCCTTGAAGTACCTCCCGAGGCGTCAACTTTTGAAGCATTTGCCAAGTTACTGGCTACACTTTCGTCATCAAAGGAGGTCCGGTCTGTTTTCTCCTTGAAAATGGCTTCTTCAAGATCAGGCAAGCAGGCTCAACAACTCAACAAATCCATATGGAACGCTCACTCAGTCATCTCGAGCTCCTTACTCGAAAGTGCTCATCTTCCGAGGAACACAAGTTACAACCAGGACCCGAACTCACCAGCCAGTTATCTCTCTGCAACCGAATTATCAACCCGAAAGAACAACGATATGAACGGATGGGATTTGGTGGAGCATCCTAAGTACCCGCCACCACCTTCACAGTCCGAGGATGTTGAACATTGGACTCGTGCTATGTTTATCGATGCCAAGAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atg14

Protein Analysis

473

Amino Acids

53.14

Weight (kDa)

9.37

Isoelectric Point (pI)

52.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG14 PF10186 8 - 367 7e-95 Vacuolar sorting 38 and autophagy-related subunit 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 662
AciI CCGC 3 cut(s) 660, 689, 1349
AclWI GGATC 3 cut(s) 6, 569, 582
AcyI GRCGYC 1 cut(s) 1043
AfaI GTAC 6 cut(s) 61, 397, 739, 911, 1032, 1346
AflII CTTAAG 1 cut(s) 239
AjiI CACGTC 1 cut(s) 557
AjnI CCWGG 2 cut(s) 889, 1245
AloI GAACNNNNNNTCC 2 cut(s) 775, 807
Alw21I GWGCWC 2 cut(s) 1202, 1219
Alw26I GTCTC 1 cut(s) 613
AlwI GGATC 3 cut(s) 6, 569, 582
AlwNI CAGNNNCTG 2 cut(s) 21, 634
Ama87I CYCGRG 3 cut(s) 679, 1037, 1195
Asp700I GAANNNNTTC 1 cut(s) 127
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 3 cut(s) 625, 1099, 1249
AsuHPI GGTGA 1 cut(s) 1251
AsuII TTCGAA 1 cut(s) 221
AvaI CYCGRG 3 cut(s) 679, 1037, 1195
AvaII GGWCC 3 cut(s) 625, 1099, 1249
BamHI GGATCC 1 cut(s) 574
BanII GRGCYC 2 cut(s) 695, 1202
BarI GAAGNNNNNNTAC 2 cut(s) 752, 784
BauI CACGAG 1 cut(s) 1389
BbsI GAAGAC 3 cut(s) 285, 428, 551
Bbv12I GWGCWC 2 cut(s) 1202, 1219
BccI CCATC 3 cut(s) 497, 598, 1314
BciT130I CCWGG 2 cut(s) 891, 1247
BciVI GTATCC 1 cut(s) 771
BclI TGATCA 1 cut(s) 181
BcoDI GTCTC 1 cut(s) 613
BfaI CTAG 1 cut(s) 354
BfmI CTRYAG 1 cut(s) 953
BfrI CTTAAG 1 cut(s) 239
BfuI GTATCC 1 cut(s) 771
BglII AGATCT 1 cut(s) 949
BisI GCNGC 1 cut(s) 660
BlsI GCNGC 1 cut(s) 661
BmcAI AGTACT 1 cut(s) 911
Bme1390I CCNGG 2 cut(s) 891, 1247
Bme18I GGWCC 3 cut(s) 625, 1099, 1249
BmeT110I CYCGRG 3 cut(s) 679, 1037, 1195
BmgBI CACGTC 1 cut(s) 557
BmgT120I GGNCC 3 cut(s) 625, 1099, 1249
BmiI GGNNCC 2 cut(s) 576, 1251
BmrFI CCNGG 2 cut(s) 891, 1247
BmsI GCATC 2 cut(s) 1345, 1396
BpiI GAAGAC 3 cut(s) 285, 428, 551
BpmI CTGGAG 2 cut(s) 814, 873
Bpu14I TTCGAA 1 cut(s) 221
BpuEI CTTGAG 3 cut(s) 249, 365, 959
Bsa29I ATCGAT 1 cut(s) 1404
BsaHI GRCGYC 1 cut(s) 1043
BsaJI CCNNGG 5 cut(s) 406, 852, 1038, 1226, 1368
BsaWI WCCGGW 1 cut(s) 1101
BsaXI ACNNNNNCTCC 2 cut(s) 1088, 1118
Bse1I ACTGG 3 cut(s) 15, 1077, 1266
Bse3DI GCAATG 1 cut(s) 760
BseBI CCWGG 2 cut(s) 891, 1247
BseCI ATCGAT 1 cut(s) 1404
BseDI CCNNGG 5 cut(s) 406, 852, 1038, 1226, 1368
BseGI GGATG 3 cut(s) 1325, 1336, 1378
BseMI GCAATG 1 cut(s) 760
BseMII CTCAG 3 cut(s) 413, 420, 1200
BseNI ACTGG 3 cut(s) 15, 1077, 1266
BseRI GAGGAG 2 cut(s) 904, 915
BsgI GTGCAG 1 cut(s) 651
BshVI ATCGAT 1 cut(s) 1404
BsiHKAI GWGCWC 2 cut(s) 1202, 1219
BsiHKCI CYCGRG 3 cut(s) 679, 1037, 1195
BsiSI CCGG 2 cut(s) 781, 1102
BslFI GGGAC 1 cut(s) 743
BsmAI GTCTC 1 cut(s) 613
BsmFI GGGAC 1 cut(s) 743
BsoBI CYCGRG 3 cut(s) 679, 1037, 1195
Bsp119I TTCGAA 1 cut(s) 221
Bsp1286I GDGCHC 3 cut(s) 695, 1202, 1219
Bsp143I GATC 6 cut(s) 11, 181, 298, 574, 949, 1136
Bsp19I CCATGG 1 cut(s) 852
BspACI CCGC 3 cut(s) 660, 689, 1349
BspCNI CTCAG 3 cut(s) 412, 421, 1199
BspDI ATCGAT 1 cut(s) 1404
BspLI GGNNCC 2 cut(s) 576, 1251
BspPI GGATC 3 cut(s) 6, 569, 582
BspQI GCTCTTC 2 cut(s) 591, 975
BspT104I TTCGAA 1 cut(s) 221
BspTI CTTAAG 1 cut(s) 239
BsrBI CCGCTC 1 cut(s) 662
BsrDI GCAATG 1 cut(s) 760
BsrI ACTGG 3 cut(s) 15, 1077, 1266
BssECI CCNNGG 5 cut(s) 406, 852, 1038, 1226, 1368
BssMI GATC 6 cut(s) 11, 181, 298, 574, 949, 1136
BssNI GRCGYC 1 cut(s) 1043
BssSI CACGAG 1 cut(s) 1389
BssT1I CCWWGG 2 cut(s) 406, 852
Bst2BI CACGAG 1 cut(s) 1389
Bst2UI CCWGG 2 cut(s) 891, 1247
Bst4CI ACNGT 2 cut(s) 521, 1365
Bst6I CTCTTC 4 cut(s) 118, 591, 773, 975
BstACI GRCGYC 1 cut(s) 1043
BstAFI CTTAAG 1 cut(s) 239
BstBI TTCGAA 1 cut(s) 221
BstC8I GCNNGC 6 cut(s) 67, 603, 691, 991, 1145, 1149
BstDEI CTNAG 6 cut(s) 165, 399, 429, 655, 1186, 1341
BstDSI CCRYGG 1 cut(s) 852
BstF5I GGATG 3 cut(s) 1325, 1336, 1378
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 6 cut(s) 14, 184, 301, 577, 952, 1139
BstMAI GTCTC 1 cut(s) 613
BstMBI GATC 6 cut(s) 11, 181, 298, 574, 949, 1136
BstMWI GCNNNNNNNGC 2 cut(s) 30, 665
BstNI CCWGG 2 cut(s) 891, 1247
BstSCI CCNGG 2 cut(s) 889, 1245
BstSFI CTRYAG 1 cut(s) 953
BstV2I GAAGAC 3 cut(s) 285, 428, 551
BstX2I RGATCY 2 cut(s) 574, 949
BstXI CCANNNNNNTGG 1 cut(s) 1072
BstYI RGATCY 2 cut(s) 574, 949
Bsu15I ATCGAT 1 cut(s) 1404
BsuI GTATCC 1 cut(s) 771
BsuTUI ATCGAT 1 cut(s) 1404
BtgI CCRYGG 1 cut(s) 852
BtrI CACGTC 1 cut(s) 557
BtsCI GGATG 3 cut(s) 1325, 1336, 1378
Cac8I GCNNGC 6 cut(s) 67, 603, 691, 991, 1145, 1149
CaiI CAGNNNCTG 2 cut(s) 21, 634
CfoI GCGC 1 cut(s) 26
Cfr13I GGNCC 3 cut(s) 625, 1099, 1249
ClaI ATCGAT 1 cut(s) 1404
CseI GACGC 2 cut(s) 422, 1032
Csp6I GTAC 6 cut(s) 60, 396, 738, 910, 1031, 1345
CviAII CATG 2 cut(s) 813, 853
CviQI GTAC 6 cut(s) 60, 396, 738, 910, 1031, 1345
DdeI CTNAG 6 cut(s) 165, 399, 429, 655, 1186, 1341
DpnI GATC 6 cut(s) 13, 183, 300, 576, 951, 1138
DpnII GATC 6 cut(s) 11, 181, 298, 574, 949, 1136
Eam1104I CTCTTC 4 cut(s) 118, 591, 773, 975
EarI CTCTTC 4 cut(s) 118, 591, 773, 975
Ecl136II GAGCTC 1 cut(s) 1200
Eco130I CCWWGG 2 cut(s) 406, 852
Eco24I GRGCYC 2 cut(s) 695, 1202
Eco47I GGWCC 3 cut(s) 625, 1099, 1249
Eco53kI GAGCTC 1 cut(s) 1200
Eco88I CYCGRG 3 cut(s) 679, 1037, 1195
EcoICRI GAGCTC 1 cut(s) 1200
EcoO109I RGGNCCY 1 cut(s) 1249
EcoRII CCWGG 2 cut(s) 889, 1245
EcoT14I CCWWGG 2 cut(s) 406, 852
EcoT38I GRGCYC 2 cut(s) 695, 1202
ErhI CCWWGG 2 cut(s) 406, 852
FaeI CATG 2 cut(s) 816, 856
FaqI GGGAC 1 cut(s) 743
FatI CATG 2 cut(s) 812, 852
FauI CCCGC 2 cut(s) 682, 1356
FauNDI CATATG 2 cut(s) 414, 1172
FbaI TGATCA 1 cut(s) 181
Fnu4HI GCNGC 1 cut(s) 660
FokI GGATG 3 cut(s) 1323, 1332, 1385
FriOI GRGCYC 2 cut(s) 695, 1202
Fsp4HI GCNGC 1 cut(s) 660
FspBI CTAG 1 cut(s) 354
GlaI GCGC 1 cut(s) 25
GluI GCNGC 1 cut(s) 660
GsuI CTGGAG 2 cut(s) 814, 873
HapII CCGG 2 cut(s) 781, 1102
HgaI GACGC 2 cut(s) 422, 1032
HhaI GCGC 1 cut(s) 26
Hin1I GRCGYC 1 cut(s) 1043
Hin1II CATG 2 cut(s) 816, 856
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HincII GTYRAC 1 cut(s) 1047
HindII GTYRAC 1 cut(s) 1047
HindIII AAGCTT 1 cut(s) 236
HpaII CCGG 2 cut(s) 781, 1102
HphI GGTGA 1 cut(s) 1251
Hpy166II GTNNAC 1 cut(s) 1047
Hpy188III TCNNGA 8 cut(s) 141, 323, 344, 881, 976, 1037, 1134, 1195
Hpy8I GTNNAC 1 cut(s) 1047
Hpy99I CGWCG 1 cut(s) 492
HpyAV CCTTC 2 cut(s) 265, 1368
HpyCH4III ACNGT 2 cut(s) 521, 1365
HpyCH4IV ACGT 3 cut(s) 487, 556, 736
HpyCH4V TGCA 4 cut(s) 471, 549, 668, 1280
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 665
HpyF3I CTNAG 6 cut(s) 165, 399, 429, 655, 1186, 1341
HpySE526I ACGT 3 cut(s) 487, 556, 736
Hsp92I GRCGYC 1 cut(s) 1043
Hsp92II CATG 2 cut(s) 816, 856
HspAI GCGC 1 cut(s) 24
Ksp22I TGATCA 1 cut(s) 181
Kzo9I GATC 6 cut(s) 11, 181, 298, 574, 949, 1136
LguI GCTCTTC 2 cut(s) 591, 975
LmnI GCTCC 3 cut(s) 667, 1205, 1333
LweI GCATC 2 cut(s) 1345, 1396
MaeI CTAG 1 cut(s) 354
MaeII ACGT 3 cut(s) 487, 556, 736
MaeIII GTNAC 7 cut(s) 17, 108, 259, 939, 1007, 1068, 1238
MalI GATC 6 cut(s) 13, 183, 300, 576, 951, 1138
MbiI CCGCTC 1 cut(s) 662
MboI GATC 6 cut(s) 11, 181, 298, 574, 949, 1136
MflI RGATCY 2 cut(s) 574, 949
MhlI GDGCHC 3 cut(s) 695, 1202, 1219
MluCI AATT 9 cut(s) 190, 385, 473, 543, 550, 784, 835, 1012, 1286
MlyI GAGTC 5 cut(s) 520, 866, 878, 947, 1381
MroXI GAANNNNTTC 1 cut(s) 127
MseI TTAA 6 cut(s) 72, 240, 276, 303, 309, 906
MspCI CTTAAG 1 cut(s) 239
MspI CCGG 2 cut(s) 781, 1102
MspR9I CCNGG 2 cut(s) 891, 1247
MvaI CCWGG 2 cut(s) 891, 1247
MwoI GCNNNNNNNGC 2 cut(s) 30, 665
NcoI CCATGG 1 cut(s) 852
NdeI CATATG 2 cut(s) 414, 1172
NdeII GATC 6 cut(s) 11, 181, 298, 574, 949, 1136
NlaIII CATG 2 cut(s) 816, 856
NlaIV GGNNCC 2 cut(s) 576, 1251
NmuCI GTSAC 2 cut(s) 108, 939
NspV TTCGAA 1 cut(s) 221
PaeR7I CTCGAG 1 cut(s) 1195
PciSI GCTCTTC 2 cut(s) 591, 975
PdmI GAANNNNTTC 1 cut(s) 127
PfeI GAWTC 7 cut(s) 128, 144, 161, 202, 326, 637, 719
PfoI TCCNGGA 1 cut(s) 889
PkrI GCNGC 1 cut(s) 661
PleI GAGTC 5 cut(s) 519, 865, 878, 946, 1381
PpsI GAGTC 5 cut(s) 519, 865, 878, 946, 1381
PpuMI RGGWCCY 1 cut(s) 1249
Psp124BI GAGCTC 1 cut(s) 1202
Psp5II RGGWCCY 1 cut(s) 1249
Psp6I CCWGG 2 cut(s) 889, 1245
PspGI CCWGG 2 cut(s) 889, 1245
PspN4I GGNNCC 2 cut(s) 576, 1251
PspPI GGNCC 3 cut(s) 625, 1099, 1249
PspPPI RGGWCCY 1 cut(s) 1249
PsrI GAACNNNNNNTAC 2 cut(s) 1223, 1255
PstNI CAGNNNCTG 2 cut(s) 21, 634
PsuI RGATCY 2 cut(s) 574, 949
RsaI GTAC 6 cut(s) 61, 397, 739, 911, 1032, 1346
RsaNI GTAC 6 cut(s) 60, 396, 738, 910, 1031, 1345
SacI GAGCTC 1 cut(s) 1202
SapI GCTCTTC 2 cut(s) 591, 975
SaqAI TTAA 6 cut(s) 72, 240, 276, 303, 309, 906
SatI GCNGC 1 cut(s) 660
Sau3AI GATC 6 cut(s) 11, 181, 298, 574, 949, 1136
Sau96I GGNCC 3 cut(s) 625, 1099, 1249
ScaI AGTACT 1 cut(s) 911
SchI GAGTC 5 cut(s) 520, 866, 878, 947, 1381
ScrFI CCNGG 2 cut(s) 891, 1247
SduI GDGCHC 3 cut(s) 695, 1202, 1219
SfaNI GCATC 2 cut(s) 1345, 1396
SfcI CTRYAG 1 cut(s) 953
Sfr274I CTCGAG 1 cut(s) 1195
SfuI TTCGAA 1 cut(s) 221
SinI GGWCC 3 cut(s) 625, 1099, 1249
SlaI CTCGAG 1 cut(s) 1195
SmlI CTYRAG 5 cut(s) 239, 264, 344, 974, 1195
SmoI CTYRAG 5 cut(s) 239, 264, 344, 974, 1195
Sse9I AATT 9 cut(s) 190, 385, 473, 543, 550, 784, 835, 1012, 1286
SsiI CCGC 3 cut(s) 660, 689, 1349
SspMI CTAG 1 cut(s) 354
SstI GAGCTC 1 cut(s) 1202
StyD4I CCNGG 2 cut(s) 889, 1245
StyI CCWWGG 2 cut(s) 406, 852
TaaI ACNGT 2 cut(s) 521, 1365
TaiI ACGT 3 cut(s) 490, 559, 739
TaqI TCGA 8 cut(s) 121, 221, 490, 748, 932, 1196, 1209, 1404
TasI AATT 9 cut(s) 190, 385, 473, 543, 550, 784, 835, 1012, 1286
TatI WGTACW 3 cut(s) 59, 395, 909
TauI GCSGC 1 cut(s) 662
TfiI GAWTC 7 cut(s) 128, 144, 161, 202, 326, 637, 719
Tru1I TTAA 6 cut(s) 72, 240, 276, 303, 309, 906
Tru9I TTAA 6 cut(s) 72, 240, 276, 303, 309, 906
TseFI GTSAC 2 cut(s) 108, 939
Tsp45I GTSAC 2 cut(s) 108, 939
TspDTI ATGAA 6 cut(s) 120, 428, 638, 711, 760, 1328
TspGWI ACGGA 2 cut(s) 747, 1332
Vha464I CTTAAG 1 cut(s) 239
VpaK11BI GGWCC 3 cut(s) 625, 1099, 1249
XhoI CTCGAG 1 cut(s) 1195
XmnI GAANNNNTTC 1 cut(s) 127
XspI CTAG 1 cut(s) 354
ZrmI AGTACT 1 cut(s) 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.