MD08G1174500.v1.1

Vacuolar sorting 38 and autophagy-related subunit 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009921
Physical Location & Seq
Reverse (-)
21203025 .. 21210319
7295 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1174500.v1.1.491

Sequence Viewer

Length: 1431 bp
ATGAACCGGAAATCTAATTGTGCTATTTGTGAGAATTCAAATCTTGCTTCTATCTGTGCTGTTTGTGTGAATTACAGATTAAATGAGTATAACAATACTCTGAAGGCACTGAAGAGTCGTCGCGAGTCATTGTATTCAAGATTGACTGAAGTGCTTGTCGCAAAGGGTAAGGCCGATGATCAAATAAATTGGAGAGTGCTTCAAAATGAGAAGCTTGTGAGGTTGAGGGAAAAACTCCGGCGTAATAAAGAACAGCTTGTGCAAGGGAAGGCTAAGATTGAGAAGGCGTCCCATGACCGTAAAGTCAAGGATGGGGTGCTTGAATCGGCCCTCTTTGCGTTGGGAAAGAATCGTGCGGAACAACTGGATAAGTTCTATCCCAACCTTATATGCACTCAGAACTTAGGGCATATGGCCATTACCTCTGAACGCCTTCATAAACAGTCAGTAGTTATAAAACAAATATGCAAATTGTTTCCCCAACGTCGGGTGAATGTAGATGAAAAGAAAAATTATGGATCTGGTGGTTTATACGATCAAATTTGCAATGCATGCTTACCAAGAGGGCTAGATCCACACTCAGTTCCATCAGAAGAGCTTGCTGCTTCCTTGGGGTACATGGTTCAACTCCTGAATCTTGTTGTTCAGAACTTAGCTGCTCCAGCACTTCATAACTCAGGTTTTGCGGGTTCTTGCTCTAGGATATGGCAAAGAGATTCTTATTGGGATGCGCGCCCTTCTTCTCGGAGCAATGAATATCCCCTATTTATACCACGCCAAAATTATTGCTCCAGTAGTGGGGAAAATTCTTGGTCTGACAAAAGCTCAAGTAATAATTTTGGTGTTGATTCAATAGAATCTGTGAGGAAACCACATCTGGATTCTTCTGGTGGTAGCTTTAATTACACTTCCGCTTCTTCACACACTGTTGAAACGCACAATGATTTGCAGAGAGGGATTTCACTTCTTAAGAAAAGTGTCGCATGCATTACAGCGTACTGCTATAACTCACTATGTTTAGATGTTCCTTCTGAGGTATCTACATTTGAAGCATTTGCAAAATTGTTGGCCACATTATCTTCTTCCAAGGAAGTTCATTCTGTGTTCTCGTTAAAATTGGCATGCTCAAGGTCATGTAAGCAAGTTCAACAACTAAACAAATCTGTGTGGAATGTGGATTCGGCCATATCGTCAACCACTCTATTGGATAGCGCACATGCAATGGCACTAACGAAAAACTTGTATGAAAAAAACCTTCCAAATTATTCCACCACCTTTCTTAGCTCCACTGAGTTGTCAGATGGGAAGAATGAATCCCTTATTGAAGAATGGGATCTTGTGGAACGTCCTACTTTTCCGCCTCCACCATCACAGCCTGAGGATATTGAGCATTGGACACGAGCCATGATCATCGATGCTAAAAGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atg14

Protein Analysis

477

Amino Acids

53.37

Weight (kDa)

8.89

Isoelectric Point (pI)

51.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG14 PF10186 7 - 364 2.1e-82 Vacuolar sorting 38 and autophagy-related subunit 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 455
AasI GACNNNNNNGTC 1 cut(s) 302
AccII CGCG 2 cut(s) 123, 733
AciI CCGC 4 cut(s) 356, 686, 912, 1358
AclWI GGATC 3 cut(s) 526, 566, 1341
AcoI YGGCCR 3 cut(s) 414, 1068, 1182
AcsI RAATTY 3 cut(s) 34, 540, 805
AcuI CTGAAG 3 cut(s) 122, 131, 168
AcyI GRCGYC 1 cut(s) 287
AfaI GTAC 2 cut(s) 617, 998
AfiI CCNNNNNNNGG 3 cut(s) 486, 487, 798
AflII CTTAAG 1 cut(s) 968
AluBI AGCT 7 cut(s) 214, 256, 598, 656, 825, 897, 1284
AluI AGCT 7 cut(s) 214, 256, 598, 656, 825, 897, 1284
AlwI GGATC 3 cut(s) 526, 566, 1341
AoxI GGCC 5 cut(s) 171, 327, 414, 1068, 1182
ApeKI GCWGC 2 cut(s) 602, 656
ApoI RAATTY 3 cut(s) 34, 540, 805
Asp700I GAANNNNTTC 1 cut(s) 432
AspLEI GCGC 3 cut(s) 733, 735, 1214
AspS9I GGNCC 1 cut(s) 328
AsuHPI GGTGA 1 cut(s) 502
AxyI CCTNAGG 1 cut(s) 1377
BalI TGGCCA 2 cut(s) 416, 1070
BauI CACGAG 1 cut(s) 1398
BbvI GCAGC 2 cut(s) 589, 643
BccI CCATC 4 cut(s) 305, 595, 1295, 1375
BclI TGATCA 2 cut(s) 178, 1407
BfaI CTAG 2 cut(s) 569, 699
BfrI CTTAAG 1 cut(s) 968
BisI GCNGC 2 cut(s) 603, 657
BlsI GCNGC 2 cut(s) 604, 658
BmgT120I GGNCC 1 cut(s) 328
BmsI GCATC 2 cut(s) 718, 1405
BpmI CTGGAG 2 cut(s) 645, 775
BpuEI CTTGAG 2 cut(s) 811, 1111
Bsa29I ATCGAT 1 cut(s) 1413
BsaHI GRCGYC 1 cut(s) 287
BsaJI CCNNGG 2 cut(s) 609, 1086
BsaWI WCCGGW 1 cut(s) 6
Bsc4I CCNNNNNNNGG 3 cut(s) 486, 487, 798
Bse1I ACTGG 2 cut(s) 369, 792
Bse21I CCTNAGG 1 cut(s) 1377
Bse3DI GCAATG 3 cut(s) 553, 757, 1227
BseCI ATCGAT 1 cut(s) 1413
BseDI CCNNGG 2 cut(s) 609, 1086
BseGI GGATG 2 cut(s) 316, 733
BseLI CCNNNNNNNGG 3 cut(s) 486, 487, 798
BseMI GCAATG 3 cut(s) 553, 757, 1227
BseMII CTCAG 6 cut(s) 410, 594, 690, 1023, 1281, 1368
BseNI ACTGG 2 cut(s) 369, 792
BsePI GCGCGC 1 cut(s) 731
BseXI GCAGC 2 cut(s) 589, 643
Bsh1236I CGCG 2 cut(s) 123, 733
BshFI GGCC 5 cut(s) 173, 329, 416, 1070, 1184
BshVI ATCGAT 1 cut(s) 1413
BsiSI CCGG 2 cut(s) 7, 238
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 3 cut(s) 486, 487, 798
BsmFI GGGAC 1 cut(s) 274
BsnI GGCC 5 cut(s) 173, 329, 416, 1070, 1184
Bsp143I GATC 6 cut(s) 178, 518, 535, 571, 1333, 1407
Bsp68I TCGCGA 1 cut(s) 123
BspACI CCGC 4 cut(s) 356, 686, 912, 1358
BspANI GGCC 5 cut(s) 173, 329, 416, 1070, 1184
BspCNI CTCAG 6 cut(s) 409, 593, 689, 1024, 1282, 1369
BspDI ATCGAT 1 cut(s) 1413
BspFNI CGCG 2 cut(s) 123, 733
BspPI GGATC 3 cut(s) 526, 566, 1341
BspQI GCTCTTC 1 cut(s) 588
BspTI CTTAAG 1 cut(s) 968
BsrDI GCAATG 3 cut(s) 553, 757, 1227
BsrI ACTGG 2 cut(s) 369, 792
BssECI CCNNGG 2 cut(s) 609, 1086
BssHII GCGCGC 1 cut(s) 731
BssMI GATC 6 cut(s) 178, 518, 535, 571, 1333, 1407
BssNI GRCGYC 1 cut(s) 287
BssSI CACGAG 1 cut(s) 1398
BssT1I CCWWGG 2 cut(s) 609, 1086
Bst2BI CACGAG 1 cut(s) 1398
Bst4CI ACNGT 3 cut(s) 299, 444, 928
Bst6I CTCTTC 2 cut(s) 107, 588
BstACI GRCGYC 1 cut(s) 287
BstAFI CTTAAG 1 cut(s) 968
BstAPI GCANNNNNTGC 1 cut(s) 552
BstC8I GCNNGC 5 cut(s) 553, 600, 733, 985, 1123
BstF5I GGATG 2 cut(s) 316, 733
BstFNI CGCG 2 cut(s) 123, 733
BstHHI GCGC 3 cut(s) 733, 735, 1214
BstKTI GATC 6 cut(s) 181, 521, 538, 574, 1336, 1410
BstMBI GATC 6 cut(s) 178, 518, 535, 571, 1333, 1407
BstMWI GCNNNNNNNGC 3 cut(s) 335, 552, 662
BstNSI RCATGY 4 cut(s) 555, 987, 1125, 1220
BstUI CGCG 2 cut(s) 123, 733
BstV1I GCAGC 2 cut(s) 589, 643
BstX2I RGATCY 3 cut(s) 518, 571, 1333
BstXI CCANNNNNNTGG 1 cut(s) 1204
BstYI RGATCY 3 cut(s) 518, 571, 1333
Bsu15I ATCGAT 1 cut(s) 1413
Bsu36I CCTNAGG 1 cut(s) 1377
BsuRI GGCC 5 cut(s) 173, 329, 416, 1070, 1184
BsuTUI ATCGAT 1 cut(s) 1413
BtsCI GGATG 2 cut(s) 316, 733
BtsIMutI CAGTG 3 cut(s) 107, 924, 1287
BtuMI TCGCGA 1 cut(s) 123
Cac8I GCNNGC 5 cut(s) 553, 600, 733, 985, 1123
CfoI GCGC 3 cut(s) 733, 735, 1214
Cfr13I GGNCC 1 cut(s) 328
ClaI ATCGAT 1 cut(s) 1413
CseI GACGC 1 cut(s) 276
Csp6I GTAC 2 cut(s) 616, 997
CviAII CATG 8 cut(s) 293, 552, 619, 984, 1122, 1134, 1217, 1405
CviQI GTAC 2 cut(s) 616, 997
DpnI GATC 6 cut(s) 180, 520, 537, 573, 1335, 1409
DpnII GATC 6 cut(s) 178, 518, 535, 571, 1333, 1407
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 3 cut(s) 414, 1068, 1182
Eam1104I CTCTTC 2 cut(s) 107, 588
EarI CTCTTC 2 cut(s) 107, 588
EciI GGCGGA 1 cut(s) 1347
Eco130I CCWWGG 2 cut(s) 609, 1086
Eco57I CTGAAG 3 cut(s) 122, 131, 168
Eco81I CCTNAGG 1 cut(s) 1377
EcoRI GAATTC 1 cut(s) 34
EcoT14I CCWWGG 2 cut(s) 609, 1086
EcoT22I ATGCAT 2 cut(s) 553, 989
ErhI CCWWGG 2 cut(s) 609, 1086
FaeI CATG 8 cut(s) 296, 555, 622, 987, 1125, 1137, 1220, 1408
FalI AAGNNNNNCTT 4 cut(s) 240, 272, 703, 735
FaqI GGGAC 1 cut(s) 274
FatI CATG 8 cut(s) 292, 551, 618, 983, 1121, 1133, 1216, 1404
FauI CCCGC 1 cut(s) 679
FauNDI CATATG 1 cut(s) 411
FbaI TGATCA 2 cut(s) 178, 1407
Fnu4HI GCNGC 2 cut(s) 603, 657
FokI GGATG 2 cut(s) 323, 740
Fsp4HI GCNGC 2 cut(s) 603, 657
FspBI CTAG 2 cut(s) 569, 699
GlaI GCGC 3 cut(s) 732, 734, 1213
GluI GCNGC 2 cut(s) 603, 657
GsuI CTGGAG 2 cut(s) 645, 775
HaeIII GGCC 5 cut(s) 173, 329, 416, 1070, 1184
HapII CCGG 2 cut(s) 7, 238
HgaI GACGC 1 cut(s) 276
HhaI GCGC 3 cut(s) 733, 735, 1214
Hin1I GRCGYC 1 cut(s) 287
Hin1II CATG 8 cut(s) 296, 555, 622, 987, 1125, 1137, 1220, 1408
Hin6I GCGC 3 cut(s) 731, 733, 1212
HinP1I GCGC 3 cut(s) 731, 733, 1212
HincII GTYRAC 1 cut(s) 1194
HindII GTYRAC 1 cut(s) 1194
HindIII AAGCTT 1 cut(s) 212
HpaII CCGG 2 cut(s) 7, 238
HphI GGTGA 1 cut(s) 502
Hpy166II GTNNAC 1 cut(s) 1194
Hpy188I TCNGA 9 cut(s) 102, 399, 427, 592, 648, 747, 817, 1033, 1300
Hpy188III TCNNGA 4 cut(s) 122, 138, 631, 878
Hpy8I GTNNAC 1 cut(s) 1194
Hpy99I CGWCG 2 cut(s) 123, 489
HpyAV CCTTC 7 cut(s) 97, 262, 277, 443, 747, 1038, 1265
HpyCH4III ACNGT 3 cut(s) 299, 444, 928
HpyCH4IV ACGT 2 cut(s) 484, 1345
HpyCH4V TGCA 9 cut(s) 262, 393, 468, 546, 551, 949, 987, 1058, 1220
HpyF10VI GCNNNNNNNGC 3 cut(s) 335, 552, 662
HpySE526I ACGT 2 cut(s) 484, 1345
Hsp92I GRCGYC 1 cut(s) 287
Hsp92II CATG 8 cut(s) 296, 555, 622, 987, 1125, 1137, 1220, 1408
HspAI GCGC 3 cut(s) 731, 733, 1212
Ksp22I TGATCA 2 cut(s) 178, 1407
Kzo9I GATC 6 cut(s) 178, 518, 535, 571, 1333, 1407
LguI GCTCTTC 1 cut(s) 588
LmnI GCTCC 4 cut(s) 664, 747, 794, 1289
Lsp1109I GCAGC 2 cut(s) 589, 643
LweI GCATC 2 cut(s) 718, 1405
MaeI CTAG 2 cut(s) 569, 699
MaeII ACGT 2 cut(s) 484, 1345
MalI GATC 6 cut(s) 180, 520, 537, 573, 1335, 1409
MboI GATC 6 cut(s) 178, 518, 535, 571, 1333, 1407
MboII GAAGA 9 cut(s) 124, 605, 732, 876, 909, 1071, 1074, 1318, 1337
MflI RGATCY 3 cut(s) 518, 571, 1333
MlsI TGGCCA 2 cut(s) 416, 1070
MluNI TGGCCA 2 cut(s) 416, 1070
MlyI GAGTC 2 cut(s) 124, 134
Mox20I TGGCCA 2 cut(s) 416, 1070
Mph1103I ATGCAT 2 cut(s) 553, 989
MroXI GAANNNNTTC 1 cut(s) 432
MscI TGGCCA 2 cut(s) 416, 1070
MseI TTAA 4 cut(s) 80, 900, 969, 1112
Msp20I TGGCCA 2 cut(s) 416, 1070
MspCI CTTAAG 1 cut(s) 968
MspI CCGG 2 cut(s) 7, 238
MvnI CGCG 2 cut(s) 123, 733
MwoI GCNNNNNNNGC 3 cut(s) 335, 552, 662
NdeI CATATG 1 cut(s) 411
NdeII GATC 6 cut(s) 178, 518, 535, 571, 1333, 1407
NlaIII CATG 8 cut(s) 296, 555, 622, 987, 1125, 1137, 1220, 1408
NruI TCGCGA 1 cut(s) 123
NsiI ATGCAT 2 cut(s) 553, 989
NspI RCATGY 4 cut(s) 555, 987, 1125, 1220
PaeI GCATGC 3 cut(s) 555, 987, 1125
PauI GCGCGC 1 cut(s) 731
PciSI GCTCTTC 1 cut(s) 588
PcsI WCGNNNNNNNCGW 1 cut(s) 1187
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 9 cut(s) 323, 349, 634, 716, 848, 857, 881, 1178, 1313
PkrI GCNGC 2 cut(s) 604, 658
PleI GAGTC 2 cut(s) 123, 133
PpsI GAGTC 2 cut(s) 123, 133
PsiI TTATAA 1 cut(s) 455
PspPI GGNCC 1 cut(s) 328
PsuI RGATCY 3 cut(s) 518, 571, 1333
PteI GCGCGC 1 cut(s) 731
RruI TCGCGA 1 cut(s) 123
RsaI GTAC 2 cut(s) 617, 998
RsaNI GTAC 2 cut(s) 616, 997
SapI GCTCTTC 1 cut(s) 588
SaqAI TTAA 4 cut(s) 80, 900, 969, 1112
SatI GCNGC 2 cut(s) 603, 657
Sau3AI GATC 6 cut(s) 178, 518, 535, 571, 1333, 1407
Sau96I GGNCC 1 cut(s) 328
SchI GAGTC 2 cut(s) 124, 134
SfaNI GCATC 2 cut(s) 718, 1405
SmlI CTYRAG 3 cut(s) 826, 968, 1126
SmoI CTYRAG 3 cut(s) 826, 968, 1126
SphI GCATGC 3 cut(s) 555, 987, 1125
SsiI CCGC 4 cut(s) 356, 686, 912, 1358
SspMI CTAG 2 cut(s) 569, 699
StyI CCWWGG 2 cut(s) 609, 1086
TaaI ACNGT 3 cut(s) 299, 444, 928
TaiI ACGT 2 cut(s) 487, 1348
TaqI TCGA 1 cut(s) 1413
TfiI GAWTC 9 cut(s) 323, 349, 634, 716, 848, 857, 881, 1178, 1313
Tru1I TTAA 4 cut(s) 80, 900, 969, 1112
Tru9I TTAA 4 cut(s) 80, 900, 969, 1112
TscAI CASTG 3 cut(s) 114, 931, 1294
TseI GCWGC 2 cut(s) 602, 656
TspDTI ATGAA 8 cut(s) 17, 425, 516, 659, 768, 1085, 1260, 1326
TspRI CASTG 3 cut(s) 114, 931, 1294
Vha464I CTTAAG 1 cut(s) 968
XapI RAATTY 3 cut(s) 34, 540, 805
XceI RCATGY 4 cut(s) 555, 987, 1125, 1220
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 2 cut(s) 569, 699
Zsp2I ATGCAT 2 cut(s) 553, 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.