MD15G1361100.v1.1

Vacuolar sorting 38 and autophagy-related subunit 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009921
Physical Location & Seq
Reverse (-)
43460079 .. 43465545
5467 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1361100.v1.1.491

Sequence Viewer

Length: 1413 bp
ATGAACCGGAAATCTAGTTGTGCTATTTGTGAGAATTCAAATCTTGCTTCTATCTGTGCTGTTTGTGTGAATTACAGATTAAATGAGTATAACAATTCGTTAAAGGTACTGAAGAGTCGTCGTGATTCATTGTATTCAAGATTGACTGAAGTGCTTGTCACAAAGGGTAAGGCCGATGATCAAATAAATTGGAGAGTGCTTCAAAATGAGAAGCTTGTGAGATTAAGGGAAAAGCTCCAGCTTAATAAAGAACAGCTTGTGCAAGGGAAGGCTAAGATTGAGAAGACATCCTATGACCTCAAAGTCAAATATGGGGTGCTTGATTCGGCCCTCTCTGTGTTGGGAAAGAATCGTGTGGAGCAACTGGATAAGTTCTATCCTAACCTTATATGCACTCAGAACTTAGGGCATATGGCCATTACCTCGGAATGCCTTCATAAGCAGTCAGTAGTTATAAAACAAATATGCAAATTATTTCCCCAACGTCGGGTGATTGTAGATGAAAAGAAGAATGATGGATCTGGTGGTCTGTATGATCAAATTTGCAATGCATGCTTACCAAGAGGGCTAGATCCACACTCAGTTCCATCAGAAGAGCTTGCTGCTTCCTTGGGCAGGTACATGGTTCAACTCCTGAATCTTGTTGTTCATAACTTAGCTGCTCCAGCACTTCATAACTCAGGTTTTGCGGGTTCTTGCTCTCGGATATGGCAAAGAGATTCTTATTGGGATGCGTACCCTTCTTCTCGGAGCAATGAATATCCCCTCTTTATACCACGCCAAAATTATTGCTCCAGTAGCGGGGAAAATTCTTGGTCTGACAGGAGCTCAAGTAATAATTTTGGTGTTGCTTCAATAGAATCCGTGAGAAAACCACGTCTGGATTCTTCTGGTGGTAGCTTTAATTATTCTTCTGCTTCTGCACACAGTGTTGAAACGCACAAAGATTTGCAGAGAGGGATTTCACTTCTTAAGAAAAGTGTGGCATGCATTACAGCGTACTGTTATAACTCACTATGTTTAGATGTCCCTTCTGAGGTATCTACATTTGAAGCATTTGCTAAATTGTTGGCCACATTATCTTCTTCCAAGGAAGTTCATTCTGTGTTCTCGTTGAAAATGGCTTGCTCGAGGTCATGTAAGCAAGTTAAACAATTAAACAAGTCTGTGTGGAATGTGAATTCTGCCATATCGTCAACCACTCTGTTGGATAGCGCACATGCAATGGCACTAATGAAAAACTTAAATGAAAACAACCTTCTTTGTTCCACTGAGTTGTCTGAAGAGAAGAATGAATCCCTTATTGAAGGATGGGATATTGTGGAACATCCGACTTTTCCCCCTCCACCATCACAGTCCGAGGATATTGAGCATTGGACTCGAGCCATGATCATCGATGCTAAAAGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atg14

Protein Analysis

471

Amino Acids

52.56

Weight (kDa)

8.77

Isoelectric Point (pI)

56.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG14 PF10186 7 - 365 8e-84 Vacuolar sorting 38 and autophagy-related subunit 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 455, 1008
AasI GACNNNNNNGTC 1 cut(s) 302
Acc36I ACCTGC 1 cut(s) 606
AciI CCGC 2 cut(s) 689, 801
AclWI GGATC 2 cut(s) 526, 566
AcoI YGGCCR 2 cut(s) 414, 1071
AcsI RAATTY 4 cut(s) 34, 540, 808, 1180
AcuI CTGAAG 3 cut(s) 131, 168, 1302
AdeI CACNNNGTG 1 cut(s) 929
AfaI GTAC 4 cut(s) 108, 620, 737, 1001
AfiI CCNNNNNNNGG 5 cut(s) 486, 487, 615, 801, 1036
AflII CTTAAG 1 cut(s) 971
AgsI TTSAA 9 cut(s) 39, 138, 203, 629, 855, 935, 1052, 1117, 1307
AjiI CACGTC 1 cut(s) 878
AluBI AGCT 8 cut(s) 214, 235, 241, 256, 598, 659, 828, 900
AluI AGCT 8 cut(s) 214, 235, 241, 256, 598, 659, 828, 900
Alw21I GWGCWC 1 cut(s) 830
AlwI GGATC 2 cut(s) 526, 566
Ama87I CYCGRG 2 cut(s) 1129, 1380
AoxI GGCC 4 cut(s) 171, 327, 414, 1071
ApeKI GCWGC 2 cut(s) 602, 659
ApoI RAATTY 4 cut(s) 34, 540, 808, 1180
Asp700I GAANNNNTTC 1 cut(s) 432
AspLEI GCGC 1 cut(s) 1217
AspS9I GGNCC 1 cut(s) 328
AsuHPI GGTGA 1 cut(s) 502
AvaI CYCGRG 2 cut(s) 1129, 1380
BalI TGGCCA 2 cut(s) 416, 1073
BanII GRGCYC 1 cut(s) 830
BbsI GAAGAC 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 830
BbvI GCAGC 2 cut(s) 589, 646
BccI CCATC 4 cut(s) 509, 595, 1305, 1357
BcgI CGANNNNNNTGC 2 cut(s) 1361, 1395
BclI TGATCA 3 cut(s) 178, 535, 1389
BfaI CTAG 2 cut(s) 15, 569
BfrI CTTAAG 1 cut(s) 971
BfuAI ACCTGC 1 cut(s) 606
BisI GCNGC 2 cut(s) 603, 660
BlsI GCNGC 2 cut(s) 604, 661
BmeT110I CYCGRG 2 cut(s) 1129, 1380
BmgBI CACGTC 1 cut(s) 878
BmgT120I GGNCC 1 cut(s) 328
BmsI GCATC 2 cut(s) 721, 1387
BpiI GAAGAC 1 cut(s) 290
BpmI CTGGAG 3 cut(s) 221, 648, 778
BpuEI CTTGAG 1 cut(s) 814
Bsa29I ATCGAT 1 cut(s) 1395
BsaJI CCNNGG 4 cut(s) 423, 609, 1089, 1359
BsaWI WCCGGW 1 cut(s) 6
BsaXI ACNNNNNCTCC 2 cut(s) 817, 847
Bsc4I CCNNNNNNNGG 5 cut(s) 486, 487, 615, 801, 1036
Bse1I ACTGG 2 cut(s) 369, 795
Bse3DI GCAATG 3 cut(s) 553, 760, 1230
BseCI ATCGAT 1 cut(s) 1395
BseDI CCNNGG 4 cut(s) 423, 609, 1089, 1359
BseGI GGATG 4 cut(s) 287, 736, 1316, 1327
BseLI CCNNNNNNNGG 5 cut(s) 486, 487, 615, 801, 1036
BseMI GCAATG 3 cut(s) 553, 760, 1230
BseMII CTCAG 5 cut(s) 410, 594, 693, 1026, 1263
BseNI ACTGG 2 cut(s) 369, 795
BseXI GCAGC 2 cut(s) 589, 646
BsgI GTGCAG 1 cut(s) 906
BshFI GGCC 4 cut(s) 173, 329, 416, 1073
BshVI ATCGAT 1 cut(s) 1395
BsiHKAI GWGCWC 1 cut(s) 830
BsiHKCI CYCGRG 2 cut(s) 1129, 1380
BsiSI CCGG 1 cut(s) 7
BslFI GGGAC 1 cut(s) 1013
BslI CCNNNNNNNGG 5 cut(s) 486, 487, 615, 801, 1036
BsmFI GGGAC 1 cut(s) 1013
BsmI GAATGC 1 cut(s) 434
BsnI GGCC 4 cut(s) 173, 329, 416, 1073
BsoBI CYCGRG 2 cut(s) 1129, 1380
Bsp1286I GDGCHC 1 cut(s) 830
Bsp143I GATC 5 cut(s) 178, 518, 535, 571, 1389
BspACI CCGC 2 cut(s) 689, 801
BspANI GGCC 4 cut(s) 173, 329, 416, 1073
BspCNI CTCAG 5 cut(s) 409, 593, 692, 1027, 1264
BspDI ATCGAT 1 cut(s) 1395
BspMI ACCTGC 1 cut(s) 606
BspPI GGATC 2 cut(s) 526, 566
BspQI GCTCTTC 1 cut(s) 588
BspTI CTTAAG 1 cut(s) 971
BsrDI GCAATG 3 cut(s) 553, 760, 1230
BsrI ACTGG 2 cut(s) 369, 795
BssECI CCNNGG 4 cut(s) 423, 609, 1089, 1359
BssMI GATC 5 cut(s) 178, 518, 535, 571, 1389
BssT1I CCWWGG 2 cut(s) 609, 1089
Bst4CI ACNGT 3 cut(s) 929, 1004, 1356
Bst6I CTCTTC 3 cut(s) 107, 588, 1278
BstAFI CTTAAG 1 cut(s) 971
BstAPI GCANNNNNTGC 1 cut(s) 552
BstC8I GCNNGC 4 cut(s) 553, 600, 988, 1126
BstDEI CTNAG 8 cut(s) 273, 396, 403, 580, 655, 679, 1035, 1272
BstENI CCTNNNNNAGG 1 cut(s) 613
BstF5I GGATG 4 cut(s) 287, 736, 1316, 1327
BstHHI GCGC 1 cut(s) 1217
BstKTI GATC 5 cut(s) 181, 521, 538, 574, 1392
BstMBI GATC 5 cut(s) 178, 518, 535, 571, 1389
BstMWI GCNNNNNNNGC 3 cut(s) 552, 665, 798
BstNSI RCATGY 3 cut(s) 555, 990, 1223
BstV1I GCAGC 2 cut(s) 589, 646
BstV2I GAAGAC 1 cut(s) 290
BstX2I RGATCY 2 cut(s) 518, 571
BstXI CCANNNNNNTGG 1 cut(s) 1207
BstYI RGATCY 2 cut(s) 518, 571
Bsu15I ATCGAT 1 cut(s) 1395
BsuRI GGCC 4 cut(s) 173, 329, 416, 1073
BsuTUI ATCGAT 1 cut(s) 1395
BtrI CACGTC 1 cut(s) 878
BtsCI GGATG 4 cut(s) 287, 736, 1316, 1327
BtsIMutI CAGTG 2 cut(s) 934, 1269
BveI ACCTGC 1 cut(s) 606
Cac8I GCNNGC 4 cut(s) 553, 600, 988, 1126
CfoI GCGC 1 cut(s) 1217
Cfr13I GGNCC 1 cut(s) 328
ClaI ATCGAT 1 cut(s) 1395
Csp6I GTAC 4 cut(s) 107, 619, 736, 1000
CviAII CATG 6 cut(s) 552, 622, 987, 1137, 1220, 1387
CviQI GTAC 4 cut(s) 107, 619, 736, 1000
DdeI CTNAG 8 cut(s) 273, 396, 403, 580, 655, 679, 1035, 1272
DpnI GATC 5 cut(s) 180, 520, 537, 573, 1391
DpnII GATC 5 cut(s) 178, 518, 535, 571, 1389
DraIII CACNNNGTG 1 cut(s) 929
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 2 cut(s) 414, 1071
Eam1104I CTCTTC 3 cut(s) 107, 588, 1278
EarI CTCTTC 3 cut(s) 107, 588, 1278
Ecl136II GAGCTC 1 cut(s) 828
Eco130I CCWWGG 2 cut(s) 609, 1089
Eco24I GRGCYC 1 cut(s) 830
Eco53kI GAGCTC 1 cut(s) 828
Eco57I CTGAAG 3 cut(s) 131, 168, 1302
Eco88I CYCGRG 2 cut(s) 1129, 1380
EcoICRI GAGCTC 1 cut(s) 828
EcoNI CCTNNNNNAGG 1 cut(s) 613
EcoRI GAATTC 2 cut(s) 34, 1180
EcoT14I CCWWGG 2 cut(s) 609, 1089
EcoT22I ATGCAT 2 cut(s) 553, 992
EcoT38I GRGCYC 1 cut(s) 830
ErhI CCWWGG 2 cut(s) 609, 1089
FaeI CATG 6 cut(s) 555, 625, 990, 1140, 1223, 1390
FalI AAGNNNNNCTT 4 cut(s) 240, 272, 706, 738
FaqI GGGAC 1 cut(s) 1013
FatI CATG 6 cut(s) 551, 621, 986, 1136, 1219, 1386
FauI CCCGC 2 cut(s) 682, 794
FauNDI CATATG 1 cut(s) 411
FbaI TGATCA 3 cut(s) 178, 535, 1389
Fnu4HI GCNGC 2 cut(s) 603, 660
FokI GGATG 4 cut(s) 274, 743, 1314, 1323
FriOI GRGCYC 1 cut(s) 830
Fsp4HI GCNGC 2 cut(s) 603, 660
FspBI CTAG 2 cut(s) 15, 569
GlaI GCGC 1 cut(s) 1216
GluI GCNGC 2 cut(s) 603, 660
GsuI CTGGAG 3 cut(s) 221, 648, 778
HaeIII GGCC 4 cut(s) 173, 329, 416, 1073
HapII CCGG 1 cut(s) 7
HhaI GCGC 1 cut(s) 1217
Hin1II CATG 6 cut(s) 555, 625, 990, 1140, 1223, 1390
Hin6I GCGC 1 cut(s) 1215
HinP1I GCGC 1 cut(s) 1215
HincII GTYRAC 1 cut(s) 1197
HindII GTYRAC 1 cut(s) 1197
HindIII AAGCTT 1 cut(s) 212
HpaII CCGG 1 cut(s) 7
HphI GGTGA 1 cut(s) 502
Hpy166II GTNNAC 1 cut(s) 1197
Hpy188III TCNNGA 4 cut(s) 122, 138, 634, 881
Hpy8I GTNNAC 1 cut(s) 1197
Hpy99I CGWCG 2 cut(s) 123, 489
HpyAV CCTTC 6 cut(s) 262, 443, 750, 1041, 1268, 1301
HpyCH4III ACNGT 3 cut(s) 929, 1004, 1356
HpyCH4IV ACGT 2 cut(s) 484, 877
HpyCH4V TGCA 9 cut(s) 262, 393, 468, 546, 551, 923, 952, 990, 1223
HpyF10VI GCNNNNNNNGC 3 cut(s) 552, 665, 798
HpyF3I CTNAG 8 cut(s) 273, 396, 403, 580, 655, 679, 1035, 1272
HpySE526I ACGT 2 cut(s) 484, 877
Hsp92II CATG 6 cut(s) 555, 625, 990, 1140, 1223, 1390
HspAI GCGC 1 cut(s) 1215
Ksp22I TGATCA 3 cut(s) 178, 535, 1389
Kzo9I GATC 5 cut(s) 178, 518, 535, 571, 1389
LguI GCTCTTC 1 cut(s) 588
LmnI GCTCC 6 cut(s) 240, 358, 667, 750, 797, 825
Lsp1109I GCAGC 2 cut(s) 589, 646
LweI GCATC 2 cut(s) 721, 1387
MaeI CTAG 2 cut(s) 15, 569
MaeII ACGT 2 cut(s) 484, 877
MaeIII GTNAC 1 cut(s) 157
MalI GATC 5 cut(s) 180, 520, 537, 573, 1391
MboI GATC 5 cut(s) 178, 518, 535, 571, 1389
MflI RGATCY 2 cut(s) 518, 571
MhlI GDGCHC 1 cut(s) 830
MlsI TGGCCA 2 cut(s) 416, 1073
MluNI TGGCCA 2 cut(s) 416, 1073
MlyI GAGTC 2 cut(s) 124, 1372
MmeI TCCRAC 2 cut(s) 1188, 1355
Mox20I TGGCCA 2 cut(s) 416, 1073
Mph1103I ATGCAT 2 cut(s) 553, 992
MroXI GAANNNNTTC 1 cut(s) 432
MscI TGGCCA 2 cut(s) 416, 1073
MseI TTAA 9 cut(s) 80, 101, 224, 243, 903, 972, 1149, 1157, 1244
Msp20I TGGCCA 2 cut(s) 416, 1073
MspCI CTTAAG 1 cut(s) 971
MspI CCGG 1 cut(s) 7
Mva1269I GAATGC 1 cut(s) 434
MwoI GCNNNNNNNGC 3 cut(s) 552, 665, 798
NdeI CATATG 1 cut(s) 411
NdeII GATC 5 cut(s) 178, 518, 535, 571, 1389
NlaIII CATG 6 cut(s) 555, 625, 990, 1140, 1223, 1390
NmuCI GTSAC 1 cut(s) 157
NsiI ATGCAT 2 cut(s) 553, 992
NspI RCATGY 3 cut(s) 555, 990, 1223
PaeI GCATGC 2 cut(s) 555, 990
PaeR7I CTCGAG 2 cut(s) 1129, 1380
PciSI GCTCTTC 1 cut(s) 588
PctI GAATGC 1 cut(s) 434
PdmI GAANNNNTTC 1 cut(s) 432
PfeI GAWTC 8 cut(s) 125, 323, 349, 637, 719, 860, 884, 1295
PkrI GCNGC 2 cut(s) 604, 661
PleI GAGTC 2 cut(s) 123, 1372
PpsI GAGTC 2 cut(s) 123, 1372
PsiI TTATAA 2 cut(s) 455, 1008
Psp124BI GAGCTC 1 cut(s) 830
PspPI GGNCC 1 cut(s) 328
PspXI VCTCGAGB 2 cut(s) 1129, 1380
PsuI RGATCY 2 cut(s) 518, 571
RsaI GTAC 4 cut(s) 108, 620, 737, 1001
RsaNI GTAC 4 cut(s) 107, 619, 736, 1000
SacI GAGCTC 1 cut(s) 830
SapI GCTCTTC 1 cut(s) 588
SaqAI TTAA 9 cut(s) 80, 101, 224, 243, 903, 972, 1149, 1157, 1244
SatI GCNGC 2 cut(s) 603, 660
Sau3AI GATC 5 cut(s) 178, 518, 535, 571, 1389
Sau96I GGNCC 1 cut(s) 328
SchI GAGTC 2 cut(s) 124, 1372
SduI GDGCHC 1 cut(s) 830
SfaNI GCATC 2 cut(s) 721, 1387
Sfr274I CTCGAG 2 cut(s) 1129, 1380
SlaI CTCGAG 2 cut(s) 1129, 1380
SmlI CTYRAG 4 cut(s) 829, 971, 1129, 1380
SmoI CTYRAG 4 cut(s) 829, 971, 1129, 1380
SphI GCATGC 2 cut(s) 555, 990
SsiI CCGC 2 cut(s) 689, 801
SspMI CTAG 2 cut(s) 15, 569
SstI GAGCTC 1 cut(s) 830
StyI CCWWGG 2 cut(s) 609, 1089
TaaI ACNGT 3 cut(s) 929, 1004, 1356
TaiI ACGT 2 cut(s) 487, 880
TaqI TCGA 3 cut(s) 1130, 1381, 1395
TfiI GAWTC 8 cut(s) 125, 323, 349, 637, 719, 860, 884, 1295
Tru1I TTAA 9 cut(s) 80, 101, 224, 243, 903, 972, 1149, 1157, 1244
Tru9I TTAA 9 cut(s) 80, 101, 224, 243, 903, 972, 1149, 1157, 1244
TscAI CASTG 2 cut(s) 934, 1276
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 2 cut(s) 602, 659
Tsp45I GTSAC 1 cut(s) 157
TspGWI ACGGA 1 cut(s) 853
TspRI CASTG 2 cut(s) 934, 1276
Vha464I CTTAAG 1 cut(s) 971
XagI CCTNNNNNAGG 1 cut(s) 613
XapI RAATTY 4 cut(s) 34, 540, 808, 1180
XceI RCATGY 3 cut(s) 555, 990, 1223
XhoI CTCGAG 2 cut(s) 1129, 1380
XmnI GAANNNNTTC 1 cut(s) 432
XspI CTAG 2 cut(s) 15, 569
Zsp2I ATGCAT 2 cut(s) 553, 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.