Rw7G040950

Vacuolar sorting 38 and autophagy-related subunit 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009921
Physical Location & Seq
Forward (+)
63892749 .. 63897514
4766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G040950.1

Sequence Viewer

Length: 1389 bp
ATGACGAACAAGAAATCCAGTAATTGTGCAATTTGTGAGAATTCGAATCTCGCATCCATCTGTGCTGTCTGTGTCAATTACAGATTGAATGATTACAACAATTCTTTGAAGGCACTAAAGAGTCGTCGCGATTCCTTGTATTCGAGATTGAGTGATGCGCTTGTCGCAAAGGGTAAGGCAGATGATCAACTAAATTGGAGAGTGCTTCAAAATGAGAAGCTCGTAAGGTTGAGGGAAAGCTCCGGCGTAATAAAGAACAACTTGTGCAAGGCTAAGATTGAGAAGACATCCTATGATCTAAAAGTCAAGTATGGGGTGCTTGAATCAGCCCTCTCTATGCTAGAAAAAAATCGTGCTGAACAACTGGAGAAGTTCTATCCTAATCTTATATGCACTCAGAGCTTAGGGCATATGGCAATTACATCTGAACGTCTTCATAAACAGTCGGTGGTTATAAAACAAATATGCAAGTTGTTTCCCCAACGTCGGGTGACTGTAGACGCAAAGAGGAAAGACGGTTCTGGTGGTCAATATGACCAGATTTGCAATGCATCGTTGCCAAGAGGGCTAGATCCACACTCAGTTCCCTCAGAAGATCTTGCTGCTTCTTTGGGATATATGGTTCAACTCTTGAATCTTGTTGTTCAGAACTTAGGTGCTCCAGCCCTTCATAACTCAGGTTTTGCGGGTTCTTGCTCTCGTATATGGCAAAGAGACTCTTATTGGGATGCACGCCCTTCTTCTCGGAGCAATGAATATCCGCTATTTATTCCACGTCAAAATTATTGCTCTACTAGTGGGAAAATTCGTGAATCAGAGAGGAAACCCCGCCTGGATTCTTCTGGAAGTAGTAGCTTTAATTATTCTTCTGCTTCTGCACATTCTGTTGAAACCCACAAAGATTTGCAGAGAGGAATTTCACTTCTCAAGAAAAGTGTGGCATGCATTACAGCATACTGTTATAACTCACTATGTTTAGATGTCCCTTCTGAGGCATCTACCTTTGAAGCATTTTCAAAGTTGCTGTCGACCTTATCTTCTTCCAAGGAAGTCCATTCAGTTTTTTCTTTAAAAATGGCTTGTTCAAGGTCATGTAAGCAAGTTCAACAATTGAACAAATCTGTATGGAATGTGAACTCTGCCATATCTTCGACCACTCTACTGGATAGTGCACATGCAATGACAATGACGAAAAACTTCTGTGAAAATAATATTCCTAATTATGCCACCAGCTTCCTTTCTTCGACTGAGATGTCTGATTCTGGGAAGAATGAATGCATGATTGAAGGGTGGGATCTTGTGGAACATCCGACTCTTCCTCCACCATCCCAATCTGAAGATATTGAGCATTGGACTCGAGCCATGATTATTGATGCAACAAAGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atg14

Protein Analysis

462

Amino Acids

51.43

Weight (kDa)

8.9

Isoelectric Point (pI)

52.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG14 PF10186 9 - 350 1.4e-82 Vacuolar sorting 38 and autophagy-related subunit 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 455, 963
AasI GACNNNNNNGTC 1 cut(s) 1252
AccI GTMKAC 2 cut(s) 498, 1028
AccII CGCG 1 cut(s) 129
AciI CCGC 3 cut(s) 686, 761, 829
AclWI GGATC 2 cut(s) 566, 1302
AcsI RAATTY 3 cut(s) 40, 804, 915
AcuI CTGAAG 1 cut(s) 1356
AfiI CCNNNNNNNGG 3 cut(s) 486, 487, 991
AhlI ACTAGT 1 cut(s) 794
AjiI CACGTC 1 cut(s) 776
AjnI CCWGG 1 cut(s) 831
AloI GAACNNNNNNTCC 5 cut(s) 31, 502, 534, 606, 638
AluBI AGCT 5 cut(s) 220, 240, 402, 855, 1233
AluI AGCT 5 cut(s) 220, 240, 402, 855, 1233
Alw21I GWGCWC 2 cut(s) 661, 1174
Alw26I GTCTC 1 cut(s) 708
Alw44I GTGCAC 1 cut(s) 1170
AlwI GGATC 2 cut(s) 566, 1302
Ama87I CYCGRG 1 cut(s) 1356
ApaLI GTGCAC 1 cut(s) 1170
ApeKI GCWGC 1 cut(s) 602
ApoI RAATTY 3 cut(s) 40, 804, 915
ArsI GACNNNNNNTTYG 2 cut(s) 1010, 1042
Asp700I GAANNNNTTC 2 cut(s) 432, 1196
AspLEI GCGC 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 502
AsuII TTCGAA 1 cut(s) 44
AvaI CYCGRG 1 cut(s) 1356
BaeGI GKGCMC 1 cut(s) 1174
BbsI GAAGAC 2 cut(s) 290, 425
Bbv12I GWGCWC 2 cut(s) 661, 1174
BbvI GCAGC 1 cut(s) 589
BccI CCATC 2 cut(s) 65, 1333
BcgI CGANNNNNNTGC 2 cut(s) 1337, 1371
BciT130I CCWGG 1 cut(s) 833
BclI TGATCA 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 708
BcuI ACTAGT 1 cut(s) 794
BfaI CTAG 3 cut(s) 341, 569, 795
BfmI CTRYAG 1 cut(s) 495
BglI GCCNNNNNGGC 1 cut(s) 565
BglII AGATCT 1 cut(s) 595
BisI GCNGC 1 cut(s) 603
BlsI GCNGC 1 cut(s) 604
Bme1390I CCNGG 1 cut(s) 833
BmeT110I CYCGRG 1 cut(s) 1356
BmgBI CACGTC 1 cut(s) 776
BmrFI CCNGG 1 cut(s) 833
BmsI GCATC 6 cut(s) 62, 145, 560, 718, 1004, 1363
BpiI GAAGAC 2 cut(s) 290, 425
BpmI CTGGAG 2 cut(s) 386, 645
Bpu10I CCTNAGC 1 cut(s) 403
Bpu14I TTCGAA 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 911
BsaJI CCNNGG 1 cut(s) 1044
BsaXI ACNNNNNCTCC 2 cut(s) 1303, 1333
Bsc4I CCNNNNNNNGG 3 cut(s) 486, 487, 991
Bse1I ACTGG 3 cut(s) 18, 369, 1167
Bse3DI GCAATG 3 cut(s) 553, 757, 1185
BseBI CCWGG 1 cut(s) 833
BseDI CCNNGG 1 cut(s) 1044
BseGI GGATG 5 cut(s) 53, 287, 733, 1306, 1325
BseLI CCNNNNNNNGG 3 cut(s) 486, 487, 991
BseMI GCAATG 3 cut(s) 553, 757, 1185
BseMII CTCAG 6 cut(s) 410, 594, 603, 690, 981, 1239
BseNI ACTGG 3 cut(s) 18, 369, 1167
BseSI GKGCMC 1 cut(s) 1174
BseXI GCAGC 1 cut(s) 589
BsgI GTGCAG 1 cut(s) 861
Bsh1236I CGCG 1 cut(s) 129
BsiHKAI GWGCWC 2 cut(s) 661, 1174
BsiHKCI CYCGRG 1 cut(s) 1356
BsiSI CCGG 1 cut(s) 243
BslFI GGGAC 1 cut(s) 968
BslI CCNNNNNNNGG 3 cut(s) 486, 487, 991
BsmAI GTCTC 1 cut(s) 708
BsmFI GGGAC 1 cut(s) 968
BsmI GAATGC 1 cut(s) 1280
BsoBI CYCGRG 1 cut(s) 1356
Bsp119I TTCGAA 1 cut(s) 44
Bsp1286I GDGCHC 2 cut(s) 661, 1174
Bsp143I GATC 5 cut(s) 184, 295, 571, 595, 1294
Bsp68I TCGCGA 1 cut(s) 129
BspACI CCGC 3 cut(s) 686, 761, 829
BspCNI CTCAG 6 cut(s) 409, 593, 602, 689, 982, 1240
BspFNI CGCG 1 cut(s) 129
BspPI GGATC 2 cut(s) 566, 1302
BspT104I TTCGAA 1 cut(s) 44
BsrDI GCAATG 3 cut(s) 553, 757, 1185
BsrI ACTGG 3 cut(s) 18, 369, 1167
BssECI CCNNGG 1 cut(s) 1044
BssMI GATC 5 cut(s) 184, 295, 571, 595, 1294
BssT1I CCWWGG 1 cut(s) 1044
Bst2UI CCWGG 1 cut(s) 833
Bst4CI ACNGT 4 cut(s) 444, 496, 518, 959
Bst6I CTCTTC 1 cut(s) 1320
BstBI TTCGAA 1 cut(s) 44
BstC8I GCNNGC 2 cut(s) 733, 943
BstDEI CTNAG 9 cut(s) 273, 396, 403, 580, 589, 652, 676, 990, 1248
BstF5I GGATG 5 cut(s) 53, 287, 733, 1306, 1325
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 1 cut(s) 160
BstKTI GATC 5 cut(s) 187, 298, 574, 598, 1297
BstMAI GTCTC 1 cut(s) 708
BstMBI GATC 5 cut(s) 184, 295, 571, 595, 1294
BstMWI GCNNNNNNNGC 3 cut(s) 164, 399, 565
BstNI CCWGG 1 cut(s) 833
BstNSI RCATGY 2 cut(s) 945, 1178
BstSCI CCNGG 1 cut(s) 831
BstSFI CTRYAG 1 cut(s) 495
BstSLI GKGCMC 1 cut(s) 1174
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 1 cut(s) 589
BstV2I GAAGAC 2 cut(s) 290, 425
BstX2I RGATCY 3 cut(s) 571, 595, 1294
BstXI CCANNNNNNTGG 1 cut(s) 1162
BstYI RGATCY 3 cut(s) 571, 595, 1294
BtrI CACGTC 1 cut(s) 776
BtsCI GGATG 5 cut(s) 53, 287, 733, 1306, 1325
BtuMI TCGCGA 1 cut(s) 129
Cac8I GCNNGC 2 cut(s) 733, 943
CfoI GCGC 1 cut(s) 160
CseI GACGC 1 cut(s) 509
CviAII CATG 6 cut(s) 942, 1092, 1175, 1279, 1363, 1386
DdeI CTNAG 9 cut(s) 273, 396, 403, 580, 589, 652, 676, 990, 1248
DpnI GATC 5 cut(s) 186, 297, 573, 597, 1296
DpnII GATC 5 cut(s) 184, 295, 571, 595, 1294
DraI TTTAAA 1 cut(s) 1071
DrdI GACNNNNNNGTC 1 cut(s) 1252
DseDI GACNNNNNNGTC 1 cut(s) 1252
Eam1104I CTCTTC 1 cut(s) 1320
EarI CTCTTC 1 cut(s) 1320
Eco130I CCWWGG 1 cut(s) 1044
Eco57I CTGAAG 1 cut(s) 1356
Eco88I CYCGRG 1 cut(s) 1356
EcoRI GAATTC 1 cut(s) 40
EcoRII CCWGG 1 cut(s) 831
EcoT14I CCWWGG 1 cut(s) 1044
EcoT22I ATGCAT 3 cut(s) 553, 947, 1280
ErhI CCWWGG 1 cut(s) 1044
FaeI CATG 6 cut(s) 945, 1095, 1178, 1282, 1366, 1389
FalI AAGNNNNNCTT 4 cut(s) 245, 277, 703, 735
FaqI GGGAC 1 cut(s) 968
FatI CATG 6 cut(s) 941, 1091, 1174, 1278, 1362, 1385
FauI CCCGC 2 cut(s) 679, 836
FauNDI CATATG 1 cut(s) 411
FbaI TGATCA 1 cut(s) 184
FblI GTMKAC 2 cut(s) 498, 1028
Fnu4HI GCNGC 1 cut(s) 603
FokI GGATG 5 cut(s) 40, 274, 740, 1293, 1312
Fsp4HI GCNGC 1 cut(s) 603
FspBI CTAG 3 cut(s) 341, 569, 795
GlaI GCGC 1 cut(s) 159
GluI GCNGC 1 cut(s) 603
GsuI CTGGAG 2 cut(s) 386, 645
HapII CCGG 1 cut(s) 243
HgaI GACGC 1 cut(s) 509
HhaI GCGC 1 cut(s) 160
Hin1II CATG 6 cut(s) 945, 1095, 1178, 1282, 1366, 1389
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HincII GTYRAC 1 cut(s) 1029
HindII GTYRAC 1 cut(s) 1029
HpaII CCGG 1 cut(s) 243
HphI GGTGA 1 cut(s) 502
Hpy166II GTNNAC 4 cut(s) 499, 1029, 1135, 1172
Hpy188III TCNNGA 6 cut(s) 128, 144, 631, 809, 843, 928
Hpy8I GTNNAC 4 cut(s) 499, 1029, 1135, 1172
Hpy99I CGWCG 2 cut(s) 129, 489
HpyAV CCTTC 5 cut(s) 103, 677, 747, 996, 1280
HpyCH4III ACNGT 4 cut(s) 444, 496, 518, 959
HpyCH4IV ACGT 3 cut(s) 430, 484, 775
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 399, 565
HpyF3I CTNAG 9 cut(s) 273, 396, 403, 580, 589, 652, 676, 990, 1248
HpySE526I ACGT 3 cut(s) 430, 484, 775
Hsp92II CATG 6 cut(s) 945, 1095, 1178, 1282, 1366, 1389
HspAI GCGC 1 cut(s) 158
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 5 cut(s) 184, 295, 571, 595, 1294
LmnI GCTCC 3 cut(s) 245, 664, 747
Lsp1109I GCAGC 1 cut(s) 589
LweI GCATC 6 cut(s) 62, 145, 560, 718, 1004, 1363
MaeI CTAG 3 cut(s) 341, 569, 795
MaeII ACGT 3 cut(s) 430, 484, 775
MaeIII GTNAC 1 cut(s) 490
MalI GATC 5 cut(s) 186, 297, 573, 597, 1296
MboI GATC 5 cut(s) 184, 295, 571, 595, 1294
MfeI CAATTG 1 cut(s) 1109
MflI RGATCY 3 cut(s) 571, 595, 1294
MhlI GDGCHC 2 cut(s) 661, 1174
MlyI GAGTC 4 cut(s) 130, 710, 1306, 1348
MmeI TCCRAC 1 cut(s) 1334
MnlI CCTC 9 cut(s) 225, 341, 501, 557, 598, 813, 905, 985, 1329
Mph1103I ATGCAT 3 cut(s) 553, 947, 1280
MroXI GAANNNNTTC 2 cut(s) 432, 1196
MseI TTAA 2 cut(s) 858, 1070
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 1 cut(s) 833
MunI CAATTG 1 cut(s) 1109
Mva1269I GAATGC 1 cut(s) 1280
MvaI CCWGG 1 cut(s) 833
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 3 cut(s) 164, 399, 565
NdeI CATATG 1 cut(s) 411
NdeII GATC 5 cut(s) 184, 295, 571, 595, 1294
NlaIII CATG 6 cut(s) 945, 1095, 1178, 1282, 1366, 1389
NmuCI GTSAC 1 cut(s) 490
NruI TCGCGA 1 cut(s) 129
NsiI ATGCAT 3 cut(s) 553, 947, 1280
NspI RCATGY 2 cut(s) 945, 1178
NspV TTCGAA 1 cut(s) 44
PaeI GCATGC 1 cut(s) 945
PaeR7I CTCGAG 1 cut(s) 1356
PctI GAATGC 1 cut(s) 1280
PdmI GAANNNNTTC 2 cut(s) 432, 1196
PfeI GAWTC 7 cut(s) 46, 131, 323, 634, 812, 836, 1259
PkrI GCNGC 1 cut(s) 604
PleI GAGTC 4 cut(s) 129, 710, 1306, 1348
PpsI GAGTC 4 cut(s) 129, 710, 1306, 1348
PsiI TTATAA 2 cut(s) 455, 963
Psp6I CCWGG 1 cut(s) 831
PspGI CCWGG 1 cut(s) 831
PspXI VCTCGAGB 1 cut(s) 1356
PsuI RGATCY 3 cut(s) 571, 595, 1294
RruI TCGCGA 1 cut(s) 129
SalI GTCGAC 1 cut(s) 1027
SaqAI TTAA 2 cut(s) 858, 1070
SatI GCNGC 1 cut(s) 603
Sau3AI GATC 5 cut(s) 184, 295, 571, 595, 1294
SchI GAGTC 4 cut(s) 130, 710, 1306, 1348
ScrFI CCNGG 1 cut(s) 833
SduI GDGCHC 2 cut(s) 661, 1174
SfaNI GCATC 6 cut(s) 62, 145, 560, 718, 1004, 1363
SfcI CTRYAG 1 cut(s) 495
Sfr274I CTCGAG 1 cut(s) 1356
SfuI TTCGAA 1 cut(s) 44
SlaI CTCGAG 1 cut(s) 1356
SmlI CTYRAG 2 cut(s) 926, 1356
SmoI CTYRAG 2 cut(s) 926, 1356
SpeI ACTAGT 1 cut(s) 794
SphI GCATGC 1 cut(s) 945
SsiI CCGC 3 cut(s) 686, 761, 829
SspI AATATT 1 cut(s) 1213
SspMI CTAG 3 cut(s) 341, 569, 795
StyD4I CCNGG 1 cut(s) 831
StyI CCWWGG 1 cut(s) 1044
TaaI ACNGT 4 cut(s) 444, 496, 518, 959
TaiI ACGT 3 cut(s) 433, 487, 778
TaqI TCGA 6 cut(s) 44, 143, 1028, 1151, 1244, 1357
TfiI GAWTC 7 cut(s) 46, 131, 323, 634, 812, 836, 1259
Tru1I TTAA 2 cut(s) 858, 1070
Tru9I TTAA 2 cut(s) 858, 1070
TseFI GTSAC 1 cut(s) 490
TseI GCWGC 1 cut(s) 602
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 4 cut(s) 425, 659, 768, 1287
VneI GTGCAC 1 cut(s) 1170
XapI RAATTY 3 cut(s) 40, 804, 915
XceI RCATGY 2 cut(s) 945, 1178
XhoI CTCGAG 1 cut(s) 1356
XmiI GTMKAC 2 cut(s) 498, 1028
XmnI GAANNNNTTC 2 cut(s) 432, 1196
XspI CTAG 3 cut(s) 341, 569, 795
Zsp2I ATGCAT 3 cut(s) 553, 947, 1280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.