Rorug07G0335000

Vacuolar sorting 38 and autophagy-related subunit 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0009921
Physical Location & Seq
Forward (+)
36766665 .. 36768540
1876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0335000.1

Sequence Viewer

Length: 441 bp
ATGAATTTTGGAACATCACTATGCCAAAGATTGAATATAAAGCATCTCGTTACCAGTACTCCTGTTTACAGCAGTGCCTCTGATGTCACAGGGGAAGGATTGAGTTTGATGTTCAGGCGCTGGGCTACTAAAAAACAAGCTGGATCCTCAAAGAATGGGCGAGACTCTTTACCTAAGAATCTTGGAGTGAAGAAATTCGGTGGAGAGAGAGTGATACCTGGAAACATCATTGTTCGCCAGAGGGGCACCCGATTCCATCCTGGAAATTATGTTGGGTTAGGGAAAGATCACACTCTTTTCGCTCTGAAAGAAGGCACCGTAAAGTTTGAAAAGCACAAGCTCAGTGGACGTAAGTGGGTGCATGTAGAGCCCAAAGAAGGTCATGTGCTTCACCCTATATATGCGAATGCTGCAGCAGCTGAGATGGTGAAGACAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atg14

Protein Analysis

146

Amino Acids

16.11

Weight (kDa)

10.29

Isoelectric Point (pI)

22.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27 PF01016 42 - 122 1.8e-36 Ribosomal L27 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 245, 314
AclWI GGATC 2 cut(s) 138, 151
AcsI RAATTY 2 cut(s) 4, 194
AfaI GTAC 1 cut(s) 58
AfiI CCNNNNNNNGG 1 cut(s) 377
AgsI TTSAA 2 cut(s) 34, 329
AjnI CCWGG 2 cut(s) 217, 259
AluBI AGCT 3 cut(s) 140, 340, 419
AluI AGCT 3 cut(s) 140, 340, 419
Alw26I GTCTC 1 cut(s) 156
AlwI GGATC 2 cut(s) 138, 151
AlwNI CAGNNNCTG 2 cut(s) 120, 419
ApeKI GCWGC 3 cut(s) 410, 413, 416
ApoI RAATTY 2 cut(s) 4, 194
Asp700I GAANNNNTTC 1 cut(s) 194
AspLEI GCGC 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 383, 439
BaeGI GKGCMC 1 cut(s) 248
BamHI GGATCC 1 cut(s) 143
BanI GGYRCC 2 cut(s) 245, 314
BanII GRGCYC 1 cut(s) 372
BbsI GAAGAC 1 cut(s) 437
BbvI GCAGC 3 cut(s) 397, 425, 428
BccI CCATC 2 cut(s) 264, 418
BciT130I CCWGG 2 cut(s) 219, 261
BcoDI GTCTC 1 cut(s) 156
BfmI CTRYAG 1 cut(s) 411
BfoI RGCGCY 1 cut(s) 121
BglI GCCNNNNNGGC 1 cut(s) 243
BisI GCNGC 3 cut(s) 411, 414, 417
BlsI GCNGC 3 cut(s) 412, 415, 418
BmcAI AGTACT 1 cut(s) 58
Bme1390I CCNGG 2 cut(s) 219, 261
BmiI GGNNCC 3 cut(s) 145, 247, 316
BmrFI CCNGG 2 cut(s) 219, 261
BmsI GCATC 1 cut(s) 52
BpiI GAAGAC 1 cut(s) 437
BsaXI ACNNNNNCTCC 4 cut(s) 43, 73, 195, 225
Bsc4I CCNNNNNNNGG 1 cut(s) 377
Bse1I ACTGG 1 cut(s) 54
BseBI CCWGG 2 cut(s) 219, 261
BseGI GGATG 1 cut(s) 256
BseLI CCNNNNNNNGG 1 cut(s) 377
BseMII CTCAG 2 cut(s) 355, 411
BseNI ACTGG 1 cut(s) 54
BseSI GKGCMC 1 cut(s) 248
BseXI GCAGC 3 cut(s) 397, 425, 428
BseYI CCCAGC 1 cut(s) 120
BshNI GGYRCC 2 cut(s) 245, 314
BslI CCNNNNNNNGG 1 cut(s) 377
BsmAI GTCTC 1 cut(s) 156
BsmI GAATGC 1 cut(s) 412
Bsp1286I GDGCHC 2 cut(s) 248, 372
Bsp143I GATC 2 cut(s) 143, 286
BspCNI CTCAG 2 cut(s) 354, 412
BspLI GGNNCC 3 cut(s) 145, 247, 316
BspMAI CTGCAG 1 cut(s) 415
BspPI GGATC 2 cut(s) 138, 151
BspT107I GGYRCC 2 cut(s) 245, 314
BsrI ACTGG 1 cut(s) 54
BssMI GATC 2 cut(s) 143, 286
Bst2UI CCWGG 2 cut(s) 219, 261
Bst4CI ACNGT 2 cut(s) 319, 436
BstDEI CTNAG 3 cut(s) 174, 341, 420
BstF5I GGATG 1 cut(s) 256
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 2 cut(s) 146, 289
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 2 cut(s) 143, 286
BstMWI GCNNNNNNNGC 4 cut(s) 243, 367, 410, 416
BstNI CCWGG 2 cut(s) 219, 261
BstNSI RCATGY 1 cut(s) 365
BstSCI CCNGG 2 cut(s) 217, 259
BstSFI CTRYAG 1 cut(s) 411
BstSLI GKGCMC 1 cut(s) 248
BstV1I GCAGC 3 cut(s) 397, 425, 428
BstV2I GAAGAC 1 cut(s) 437
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BtsCI GGATG 1 cut(s) 256
BtsI GCAGTG 1 cut(s) 79
BtsIMutI CAGTG 2 cut(s) 79, 349
CaiI CAGNNNCTG 2 cut(s) 120, 419
CfoI GCGC 1 cut(s) 120
Csp6I GTAC 1 cut(s) 57
CspCI CAANNNNNGTGG 2 cut(s) 325, 360
CviAII CATG 2 cut(s) 362, 383
CviJI RGCY 5 cut(s) 125, 140, 340, 370, 419
CviKI_1 RGCY 5 cut(s) 125, 140, 340, 370, 419
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 3 cut(s) 174, 341, 420
DpnI GATC 2 cut(s) 145, 288
DpnII GATC 2 cut(s) 143, 286
Eco24I GRGCYC 1 cut(s) 372
EcoRII CCWGG 2 cut(s) 217, 259
EcoT38I GRGCYC 1 cut(s) 372
FaeI CATG 2 cut(s) 365, 386
FaiI YATR 8 cut(s) 22, 38, 270, 363, 384, 398, 400, 402
FatI CATG 2 cut(s) 361, 382
Fnu4HI GCNGC 3 cut(s) 411, 414, 417
FokI GGATG 1 cut(s) 243
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 3 cut(s) 411, 414, 417
GlaI GCGC 1 cut(s) 119
GluI GCNGC 3 cut(s) 411, 414, 417
GsaI CCCAGC 1 cut(s) 124
HaeII RGCGCY 1 cut(s) 121
HhaI GCGC 1 cut(s) 120
Hin1II CATG 2 cut(s) 365, 386
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HinfI GANTC 3 cut(s) 164, 178, 252
HphI GGTGA 2 cut(s) 383, 439
Hpy166II GTNNAC 2 cut(s) 67, 347
Hpy188I TCNGA 3 cut(s) 82, 306, 440
Hpy8I GTNNAC 2 cut(s) 67, 347
HpyAV CCTTC 3 cut(s) 89, 305, 371
HpyCH4III ACNGT 2 cut(s) 319, 436
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 2 cut(s) 361, 413
HpyF10VI GCNNNNNNNGC 4 cut(s) 243, 367, 410, 416
HpyF3I CTNAG 3 cut(s) 174, 341, 420
HpySE526I ACGT 1 cut(s) 349
Hsp92II CATG 2 cut(s) 365, 386
HspAI GCGC 1 cut(s) 118
Kzo9I GATC 2 cut(s) 143, 286
Lsp1109I GCAGC 3 cut(s) 397, 425, 428
LweI GCATC 1 cut(s) 52
MaeII ACGT 1 cut(s) 349
MaeIII GTNAC 2 cut(s) 49, 85
MalI GATC 2 cut(s) 145, 288
MboI GATC 2 cut(s) 143, 286
MboII GAAGA 1 cut(s) 202
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 2 cut(s) 248, 372
MluCI AATT 3 cut(s) 4, 194, 265
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 3 cut(s) 88, 157, 234
MroXI GAANNNNTTC 1 cut(s) 194
MslI CAYNNNNRTG 1 cut(s) 19
MspA1I CMGCKG 1 cut(s) 419
MspR9I CCNGG 2 cut(s) 219, 261
Mva1269I GAATGC 1 cut(s) 412
MvaI CCWGG 2 cut(s) 219, 261
MwoI GCNNNNNNNGC 4 cut(s) 243, 367, 410, 416
NdeII GATC 2 cut(s) 143, 286
NlaIII CATG 2 cut(s) 365, 386
NlaIV GGNNCC 3 cut(s) 145, 247, 316
NmuCI GTSAC 1 cut(s) 85
NspI RCATGY 1 cut(s) 365
PctI GAATGC 1 cut(s) 412
PdmI GAANNNNTTC 1 cut(s) 194
PfeI GAWTC 2 cut(s) 178, 252
PfoI TCCNGGA 1 cut(s) 259
PkrI GCNGC 3 cut(s) 412, 415, 418
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
Psp6I CCWGG 2 cut(s) 217, 259
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 2 cut(s) 217, 259
PspN4I GGNNCC 3 cut(s) 145, 247, 316
PstI CTGCAG 1 cut(s) 415
PstNI CAGNNNCTG 2 cut(s) 120, 419
PsuI RGATCY 1 cut(s) 143
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
RseI CAYNNNNRTG 1 cut(s) 19
SatI GCNGC 3 cut(s) 411, 414, 417
Sau3AI GATC 2 cut(s) 143, 286
ScaI AGTACT 1 cut(s) 58
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 2 cut(s) 219, 261
SduI GDGCHC 2 cut(s) 248, 372
SetI ASST 7 cut(s) 142, 175, 220, 342, 352, 382, 421
SfaNI GCATC 1 cut(s) 52
SfcI CTRYAG 1 cut(s) 411
SmiMI CAYNNNNRTG 1 cut(s) 19
Sse9I AATT 3 cut(s) 4, 194, 265
StyD4I CCNGG 2 cut(s) 217, 259
TaaI ACNGT 2 cut(s) 319, 436
TaiI ACGT 1 cut(s) 352
TasI AATT 3 cut(s) 4, 194, 265
TatI WGTACW 1 cut(s) 56
TfiI GAWTC 2 cut(s) 178, 252
TscAI CASTG 2 cut(s) 79, 349
TseFI GTSAC 1 cut(s) 85
TseI GCWGC 3 cut(s) 410, 413, 416
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 79, 349
XapI RAATTY 2 cut(s) 4, 194
XceI RCATGY 1 cut(s) 365
XmnI GAANNNNTTC 1 cut(s) 194
ZrmI AGTACT 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.