RLG00000000683

Vacuolar sorting 38 and autophagy-related subunit 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009921
Physical Location & Seq
Reverse (-)
3768922 .. 3772149
3228 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000683

Sequence Viewer

Length: 1020 bp
ATGGCAATTACATCTGAACGTCTTCATAAACAGTCGGTGGTTATAAAACAAATATGCAAGTTGTTTCCCCAACGTCGGGTGACTGTAGACGCAAAGAGGAAAGACGGTTCTGGTGGTCAATATGACCAGATTTGCAATGCATCGTTGCCAAGAGGGCTAGATCCACACTCAGTTCCCTCAGAAGATCTTGCTGCTTCTTTGGGATATATGGTTCAACTCTTGAATCTTGTTGTTCAGAACTTAGGTGCTCCAGCCCTTCATAACTCAGGTTTTGCGGGTTCTTGCTCTCGTATATGGCAAAGAGATTCTTATTGGGATGCACGCCCTTCTTCTCGGAGCAATGAATATCCGCTATTTATTCCACGTCAAAATTATTGCTCTACTAGTGGGGAAAATTCGTGGTCTGATAGAAGCTCGAGTAATTTTGGTGTTGCTTCAATAGAATCAGAGAGGAAACCCCGTCTGGATTCGTCTGGAAGTAGTAGCTTTAATTATTCTTCTGCTTCTGCACATTCTGTTGAAACCCACAAAGATTTGCAGAGAGGAATTTCACTTCTCAAGAAAAGTGTGGCATGCATTACAGCATACTGTTATAACTCACTATGTTTAGATGTCCCTTCTGAGGCATCTACCTTTGAAGCATTTGCAAAGTTGCTGTCGACCTTATCTTCTTCCAAGGAAGTCCATTCAGTTTTTTATTTAAAAATGGCTTGTTCAAGGTCATGTAAGCAAGTTCAACAATTGAACAAATCTGTATGGAATGTGAACTCTGCGATATCTTCGACCACTCTACTGGATAGTGCACATGCAATGACAATGACGAAAAACTTCTGTGAAAATAATATTCCTAATTATGCCACCAGCTTCCTTTCTTCGACTGAGATGTCTGATTCTGGGAAGAATGAATGCATGATTGAAGGGTGGGATCTTGTGGAACATCCGACTCTTCCTCCACCATCCCAATCTGAAGATATTGAGCATTGGACTCGAGCGATGTTTATTGATGCAACAAAGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atg14

Protein Analysis

340

Amino Acids

37.46

Weight (kDa)

7.08

Isoelectric Point (pI)

63.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG14 PF10186 1 - 227 4.1e-58 Vacuolar sorting 38 and autophagy-related subunit 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 44, 594
AasI GACNNNNNNGTC 1 cut(s) 883
AccI GTMKAC 2 cut(s) 87, 659
AciI CCGC 2 cut(s) 275, 350
AclWI GGATC 2 cut(s) 155, 933
AcsI RAATTY 2 cut(s) 394, 546
AcuI CTGAAG 1 cut(s) 987
AfiI CCNNNNNNNGG 3 cut(s) 75, 76, 622
AgsI TTSAA 9 cut(s) 215, 223, 438, 521, 638, 717, 737, 745, 917
AhlI ACTAGT 1 cut(s) 383
AjiI CACGTC 1 cut(s) 365
AloI GAACNNNNNNTCC 4 cut(s) 91, 123, 195, 227
AluBI AGCT 3 cut(s) 414, 486, 864
AluI AGCT 3 cut(s) 414, 486, 864
Alw21I GWGCWC 2 cut(s) 250, 805
Alw44I GTGCAC 1 cut(s) 801
AlwI GGATC 2 cut(s) 155, 933
Ama87I CYCGRG 2 cut(s) 415, 987
ApaLI GTGCAC 1 cut(s) 801
ApeKI GCWGC 1 cut(s) 191
ApoI RAATTY 2 cut(s) 394, 546
ArsI GACNNNNNNTTYG 2 cut(s) 641, 673
Asp700I GAANNNNTTC 2 cut(s) 21, 827
AsuHPI GGTGA 1 cut(s) 91
AvaI CYCGRG 2 cut(s) 415, 987
BaeGI GKGCMC 1 cut(s) 805
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 2 cut(s) 250, 805
BbvI GCAGC 1 cut(s) 178
BccI CCATC 1 cut(s) 964
BcgI CGANNNNNNTGC 2 cut(s) 968, 1002
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 2 cut(s) 158, 384
BfmI CTRYAG 1 cut(s) 84
BglI GCCNNNNNGGC 1 cut(s) 154
BglII AGATCT 1 cut(s) 184
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
BmeT110I CYCGRG 2 cut(s) 415, 987
BmgBI CACGTC 1 cut(s) 365
BmsI GCATC 4 cut(s) 149, 307, 635, 994
BpiI GAAGAC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 542
BsaJI CCNNGG 1 cut(s) 675
BsaXI ACNNNNNCTCC 2 cut(s) 934, 964
Bsc4I CCNNNNNNNGG 3 cut(s) 75, 76, 622
Bse1I ACTGG 1 cut(s) 798
Bse3DI GCAATG 3 cut(s) 142, 346, 816
BseDI CCNNGG 1 cut(s) 675
BseGI GGATG 3 cut(s) 322, 937, 956
BseLI CCNNNNNNNGG 3 cut(s) 75, 76, 622
BseMI GCAATG 3 cut(s) 142, 346, 816
BseMII CTCAG 5 cut(s) 183, 192, 279, 612, 870
BseNI ACTGG 1 cut(s) 798
BseSI GKGCMC 1 cut(s) 805
BseXI GCAGC 1 cut(s) 178
BsgI GTGCAG 1 cut(s) 492
BsiHKAI GWGCWC 2 cut(s) 250, 805
BsiHKCI CYCGRG 2 cut(s) 415, 987
BslFI GGGAC 1 cut(s) 599
BslI CCNNNNNNNGG 3 cut(s) 75, 76, 622
BsmFI GGGAC 1 cut(s) 599
BsmI GAATGC 1 cut(s) 911
BsoBI CYCGRG 2 cut(s) 415, 987
Bsp1286I GDGCHC 2 cut(s) 250, 805
Bsp143I GATC 3 cut(s) 160, 184, 925
BspACI CCGC 2 cut(s) 275, 350
BspCNI CTCAG 5 cut(s) 182, 191, 278, 613, 871
BspPI GGATC 2 cut(s) 155, 933
BsrDI GCAATG 3 cut(s) 142, 346, 816
BsrI ACTGG 1 cut(s) 798
BssECI CCNNGG 1 cut(s) 675
BssMI GATC 3 cut(s) 160, 184, 925
BssT1I CCWWGG 1 cut(s) 675
Bst4CI ACNGT 4 cut(s) 33, 85, 107, 590
Bst6I CTCTTC 1 cut(s) 951
BstC8I GCNNGC 2 cut(s) 322, 574
BstDEI CTNAG 6 cut(s) 169, 178, 241, 265, 621, 879
BstF5I GGATG 3 cut(s) 322, 937, 956
BstKTI GATC 3 cut(s) 163, 187, 928
BstMBI GATC 3 cut(s) 160, 184, 925
BstMWI GCNNNNNNNGC 1 cut(s) 154
BstNSI RCATGY 2 cut(s) 576, 809
BstSFI CTRYAG 1 cut(s) 84
BstSLI GKGCMC 1 cut(s) 805
BstV1I GCAGC 1 cut(s) 178
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 3 cut(s) 160, 184, 925
BstXI CCANNNNNNTGG 1 cut(s) 793
BstYI RGATCY 3 cut(s) 160, 184, 925
BtgZI GCGATG 1 cut(s) 1007
BtrI CACGTC 1 cut(s) 365
BtsCI GGATG 3 cut(s) 322, 937, 956
Cac8I GCNNGC 2 cut(s) 322, 574
CseI GACGC 1 cut(s) 98
CviAII CATG 5 cut(s) 573, 723, 806, 910, 1017
CviJI RGCY 6 cut(s) 157, 254, 414, 486, 710, 864
CviKI_1 RGCY 6 cut(s) 157, 254, 414, 486, 710, 864
DdeI CTNAG 6 cut(s) 169, 178, 241, 265, 621, 879
DpnI GATC 3 cut(s) 162, 186, 927
DpnII GATC 3 cut(s) 160, 184, 925
DraI TTTAAA 1 cut(s) 702
DrdI GACNNNNNNGTC 1 cut(s) 883
DseDI GACNNNNNNGTC 1 cut(s) 883
Eam1104I CTCTTC 1 cut(s) 951
EarI CTCTTC 1 cut(s) 951
Eco130I CCWWGG 1 cut(s) 675
Eco32I GATATC 1 cut(s) 777
Eco57I CTGAAG 1 cut(s) 987
Eco88I CYCGRG 2 cut(s) 415, 987
EcoRV GATATC 1 cut(s) 777
EcoT14I CCWWGG 1 cut(s) 675
EcoT22I ATGCAT 3 cut(s) 142, 578, 911
ErhI CCWWGG 1 cut(s) 675
FaeI CATG 5 cut(s) 576, 726, 809, 913, 1020
FalI AAGNNNNNCTT 2 cut(s) 292, 324
FaqI GGGAC 1 cut(s) 599
FatI CATG 5 cut(s) 572, 722, 805, 909, 1016
FauI CCCGC 1 cut(s) 268
FblI GTMKAC 2 cut(s) 87, 659
Fnu4HI GCNGC 1 cut(s) 192
FokI GGATG 3 cut(s) 329, 924, 943
Fsp4HI GCNGC 1 cut(s) 192
FspBI CTAG 2 cut(s) 158, 384
GluI GCNGC 1 cut(s) 192
GsuI CTGGAG 1 cut(s) 234
HgaI GACGC 1 cut(s) 98
Hin1II CATG 5 cut(s) 576, 726, 809, 913, 1020
HincII GTYRAC 1 cut(s) 660
HindII GTYRAC 1 cut(s) 660
HinfI GANTC 7 cut(s) 223, 305, 443, 467, 890, 943, 985
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 4 cut(s) 88, 660, 766, 803
Hpy188III TCNNGA 4 cut(s) 220, 464, 474, 559
Hpy8I GTNNAC 4 cut(s) 88, 660, 766, 803
Hpy99I CGWCG 1 cut(s) 78
HpyAV CCTTC 4 cut(s) 266, 336, 627, 911
HpyCH4III ACNGT 4 cut(s) 33, 85, 107, 590
HpyCH4IV ACGT 3 cut(s) 19, 73, 364
HpyF10VI GCNNNNNNNGC 1 cut(s) 154
HpyF3I CTNAG 6 cut(s) 169, 178, 241, 265, 621, 879
HpySE526I ACGT 3 cut(s) 19, 73, 364
Hsp92II CATG 5 cut(s) 576, 726, 809, 913, 1020
Kzo9I GATC 3 cut(s) 160, 184, 925
LmnI GCTCC 2 cut(s) 253, 336
LpnPI CCDG 9 cut(s) 96, 140, 252, 264, 449, 459, 779, 874, 879
Lsp1109I GCAGC 1 cut(s) 178
LweI GCATC 4 cut(s) 149, 307, 635, 994
MaeI CTAG 2 cut(s) 158, 384
MaeII ACGT 3 cut(s) 19, 73, 364
MaeIII GTNAC 1 cut(s) 79
MalI GATC 3 cut(s) 162, 186, 927
MboI GATC 3 cut(s) 160, 184, 925
MfeI CAATTG 1 cut(s) 740
MflI RGATCY 3 cut(s) 160, 184, 925
MhlI GDGCHC 2 cut(s) 250, 805
MluCI AATT 8 cut(s) 6, 370, 394, 421, 490, 546, 740, 850
MlyI GAGTC 2 cut(s) 937, 979
MmeI TCCRAC 1 cut(s) 965
MnlI CCTC 7 cut(s) 90, 146, 187, 444, 536, 616, 960
Mph1103I ATGCAT 3 cut(s) 142, 578, 911
MroXI GAANNNNTTC 2 cut(s) 21, 827
MseI TTAA 2 cut(s) 489, 701
MunI CAATTG 1 cut(s) 740
Mva1269I GAATGC 1 cut(s) 911
MwoI GCNNNNNNNGC 1 cut(s) 154
NdeII GATC 3 cut(s) 160, 184, 925
NlaIII CATG 5 cut(s) 576, 726, 809, 913, 1020
NmuCI GTSAC 1 cut(s) 79
NsiI ATGCAT 3 cut(s) 142, 578, 911
NspI RCATGY 2 cut(s) 576, 809
PaeI GCATGC 1 cut(s) 576
PaeR7I CTCGAG 2 cut(s) 415, 987
PctI GAATGC 1 cut(s) 911
PdmI GAANNNNTTC 2 cut(s) 21, 827
PfeI GAWTC 5 cut(s) 223, 305, 443, 467, 890
PkrI GCNGC 1 cut(s) 193
PleI GAGTC 2 cut(s) 937, 979
PpsI GAGTC 2 cut(s) 937, 979
PsiI TTATAA 2 cut(s) 44, 594
PspXI VCTCGAGB 2 cut(s) 415, 987
PsuI RGATCY 3 cut(s) 160, 184, 925
SalI GTCGAC 1 cut(s) 658
SaqAI TTAA 2 cut(s) 489, 701
SatI GCNGC 1 cut(s) 192
Sau3AI GATC 3 cut(s) 160, 184, 925
SchI GAGTC 2 cut(s) 937, 979
SduI GDGCHC 2 cut(s) 250, 805
SfaNI GCATC 4 cut(s) 149, 307, 635, 994
SfcI CTRYAG 1 cut(s) 84
Sfr274I CTCGAG 2 cut(s) 415, 987
SlaI CTCGAG 2 cut(s) 415, 987
SmlI CTYRAG 3 cut(s) 415, 557, 987
SmoI CTYRAG 3 cut(s) 415, 557, 987
SpeI ACTAGT 1 cut(s) 383
SphI GCATGC 1 cut(s) 576
Sse9I AATT 8 cut(s) 6, 370, 394, 421, 490, 546, 740, 850
SsiI CCGC 2 cut(s) 275, 350
SspI AATATT 1 cut(s) 844
SspMI CTAG 2 cut(s) 158, 384
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 4 cut(s) 33, 85, 107, 590
TaiI ACGT 3 cut(s) 22, 76, 367
TaqI TCGA 5 cut(s) 416, 659, 782, 875, 988
TasI AATT 8 cut(s) 6, 370, 394, 421, 490, 546, 740, 850
TfiI GAWTC 5 cut(s) 223, 305, 443, 467, 890
Tru1I TTAA 2 cut(s) 489, 701
Tru9I TTAA 2 cut(s) 489, 701
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 4 cut(s) 14, 248, 357, 918
VneI GTGCAC 1 cut(s) 801
XapI RAATTY 2 cut(s) 394, 546
XceI RCATGY 2 cut(s) 576, 809
XhoI CTCGAG 2 cut(s) 415, 987
XmiI GTMKAC 2 cut(s) 87, 659
XmnI GAANNNNTTC 2 cut(s) 21, 827
XspI CTAG 2 cut(s) 158, 384
Zsp2I ATGCAT 3 cut(s) 142, 578, 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.