Rh7DG473400

Vacuolar sorting 38 and autophagy-related subunit 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0009921
Physical Location & Seq
Forward (+)
67724501 .. 67734423
9923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG473400.1

Sequence Viewer

Length: 2475 bp
ATGGAGGTTTCTACAGCCACACTCCAACCCTCTTGCTCTTTCCCGCTAAGAGCCCCTAAAACAATTACCAGTGACGGCGGTTCTTCTCTATCATTCATCAGAACTGCGTCTGAAAACTTCTCCCTATCCTCTAAACCCACCAAAGGTTTTCAGTTACTAGTTAAACCGGTCAGGTTGCGGCGGCTATCGGTTGTTGCAGCTGTTGTTGGTGATAAAGCTTCAGTGCCAAGTGATTGTTCTAGTGGGGAAGAGTTGTCTATTTCCGATTCTTCTGATCCTACTTTGGTTGGAGATGAAGATGGAGGAAATGGTGAAAAGGGGGAAGGGGAAGGTGGTGTTGGGTCTTTGGATGACCACAAAATGACCCGAATATGCGATAAGCTTATTGAGGTTTTCATGGTTGACAAGCCCACTCCAACTGACTGGAGAAGATTATTGGCTTTCAGTAGGGAATGGGATGATATCAGGCCTCATTTCTTTAAGCGCTGTCAGGTCCGAGCAGATGCTGAGGATGATCCTGGAATGAAGCATAAGATACTTCGGCTTGGGCGGAAATTGAAGGAGATTGATGAAGACGTGCAAAGACACAATGAACTGCTTAAAGTTGTTATAGGGGCTCCATCAGATATCAGTGACATAGTTGCCAGACGTCGCAAAGATTTCACAAAAGAATTTTTTGTGCATCTTCACACAGTTTCTGAGTCCTATTATGAGAATCTAGAAGAGCAAAATGCTTTGGCAAGCATTGGAAATACGTGCTTGGCAGCTGTACAAGCATATGATTCTGCAACCGAAAGCATTGAAGCACTGAATGCTGCAGAGTTGAAATTTCAAGATATTATTGACTCTCCTTCTGTTGATGCTGCTTGTAGGAAGATAGACCACTTGGCTGAGAAAAATCAACTCGACTCAGCATTGGTTATGATGATTACAAAAGCTTGGTCAGCTGCTAAGGAATCGAGCATGACGAAAGATGAGGTAAAAGATGTATTATATCATTTGTATGTAACTGCAAGAGGTAATCTTCAGAAGCTCATGCCAAAAGAAATTCGGATAGTTAAGTATCTGCTCACAATTGACGATCCTGAGGAGCGGCTGTCTGCCTTACAAGATGCATTTACCCCGGGAGAGGAACTTGAAGGGAAGGATGTAGATAACCTCTACACATTGAATGATTACAACAATTCTTTAAAGGCACTAAAGAGTCGTCGCGATTCCTTGTATTCGAGATTGAGTGATGCGCTTGTCGCAAAGGGTAAGGCAGATGATCAACTAAATTGGAGAGTGCTTCAAAATGAGAAGCTTGTGAGGTTGAGGGAAAAGCTCCGGCGTAATAAAGAACAACTTGCGCAAGGGAAGGCTAAGATTGAGAAGACATCCTATGATCTAAAAGTCAAGTATGGGGTGCTTGAATCAGCCCTCTCTATGCTAGAAAAAAATCGTGCTGAACAACTGGAGAAGTTCTATCCTAATCTTATATGCACTCAGAGCTTAGGGCATATGGCAATTACATCTGAACGTCTTCATAAACAGTCGGTGGTTATAAAACAAATATGCAAGTTGTTTCCCCAACGTCGGGTGACTGTAGACGCAAAGAGGAAAGAGGGTTCTGGTGGTCAATATGACCAGATTTGCAATGCATCGTTGCCAAGAGGGCTAGATCCACACTCAGTTCCCTCAGAAGACCTTGCTGCTTCTTTGGGATATATGGTTCAACTCTTGAATCTTGTTGTTCAGAACTTAGGTGCTCCAGCCCTTCATAACTCAGGTTTTGCGGGTTCTTGCTCTCGTATATGGCAAAGAGATTCTTATTGGGATGCACGCCCTTCTTCTCGGAGCAATGAATATCCGCTATTTATTCCACGTCAAAATTATTGCTCTACTAGTGGGGAAAATTCGTGGTCTGATAGAAGCTCGAGTAATTTTGGTGTTGCTTCAATAGAATCAGAGAGGAAACCCTGTCTGGATTCTTCTGGAAGTAGTAGCTTTAATTATTCTTCTGCTTCTGCACATTCTGTTGAAACCCACAAAGATTTGCAGAGAGGAATTTCACTTCTCAAGAAAAGTGTGGCATGCATTACAGCATACTGTTATAACTCACTATGTTTAGATGTCCCGTCTGAGGCATCTACCTTTGAAGCATTTGCAAAGTTGCTGTCGACCTTATCTTCTTCCAAGGAAGTCCATTCAGTTTTTTATTTAAAAATGGCTTGTTCAAGGTCATGTAAGCAAGTTCAACAATTGAACAAATCTGTATGGAATGTGAACTCTGCCATATCTTCGACCACTCTACTGGATAGTGCACATGCAATGACAATGACGAAAAACTTCTGCGAAAATAATATTCCTAATTATGCCACCAGCTTCCTTTCTTCGACTGAGATGTCTGATTCTGGGAAGAATGAATGCATGATTGAAGGACATGATTTGGTGCTAATGCAGTCTTTCTTATTGTATGTCGAAGTTGATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atg14

Protein Analysis

824

Amino Acids

91.71

Weight (kDa)

6.25

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG14 PF10186 389 - 727 6.3e-80 Vacuolar sorting 38 and autophagy-related subunit 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1544, 2094
AasI GACNNNNNNGTC 1 cut(s) 2383
AatII GACGTC 1 cut(s) 652
Acc16I TGCGCA 1 cut(s) 1350
AccBSI CCGCTC 1 cut(s) 1093
AccI GTMKAC 2 cut(s) 1587, 2159
AccII CGCG 1 cut(s) 1212
AciI CCGC 8 cut(s) 44, 78, 178, 181, 550, 1093, 1775, 1850
AclWI GGATC 4 cut(s) 269, 509, 1076, 1655
AcsI RAATTY 5 cut(s) 671, 827, 1047, 1894, 2046
AcuI CTGAAG 2 cut(s) 204, 1010
AcyI GRCGYC 1 cut(s) 649
AfaI GTAC 1 cut(s) 771
AfeI AGCGCT 1 cut(s) 485
AfiI CCNNNNNNNGG 5 cut(s) 143, 1129, 1575, 1576, 2122
AgeI ACCGGT 1 cut(s) 166
AhlI ACTAGT 2 cut(s) 157, 1883
AjiI CACGTC 2 cut(s) 577, 1865
AjnI CCWGG 1 cut(s) 517
AjuI GAANNNNNNNTTGG 4 cut(s) 220, 252, 321, 353
AloI GAACNNNNNNTCC 4 cut(s) 1591, 1623, 1695, 1727
Alw21I GWGCWC 2 cut(s) 1750, 2305
Alw44I GTGCAC 1 cut(s) 2301
AlwI GGATC 4 cut(s) 269, 509, 1076, 1655
AlwNI CAGNNNCTG 2 cut(s) 506, 698
Ama87I CYCGRG 2 cut(s) 1123, 1915
Aor51HI AGCGCT 1 cut(s) 485
AoxI GGCC 1 cut(s) 467
ApaLI GTGCAC 1 cut(s) 2301
ApeKI GCWGC 6 cut(s) 197, 764, 815, 863, 947, 1691
ApoI RAATTY 5 cut(s) 671, 827, 1047, 1894, 2046
ArsI GACNNNNNNTTYG 4 cut(s) 926, 958, 2141, 2173
AsiGI ACCGGT 1 cut(s) 166
Asp700I GAANNNNTTC 2 cut(s) 1521, 2327
AspLEI GCGC 3 cut(s) 486, 1243, 1351
AspS9I GGNCC 1 cut(s) 493
AsuC2I CCSGG 2 cut(s) 1124, 1125
AsuHPI GGTGA 3 cut(s) 221, 323, 1591
AvaI CYCGRG 2 cut(s) 1123, 1915
AvaII GGWCC 1 cut(s) 493
AxyI CCTNAGG 1 cut(s) 1086
BaeGI GKGCMC 1 cut(s) 2305
BanII GRGCYC 2 cut(s) 55, 619
BbsI GAAGAC 4 cut(s) 579, 1379, 1514, 1689
Bbv12I GWGCWC 2 cut(s) 1750, 2305
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 6 cut(s) 209, 776, 802, 850, 934, 1678
BccI CCATC 2 cut(s) 293, 628
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 1 cut(s) 519
BclI TGATCA 1 cut(s) 1267
BcnI CCSGG 2 cut(s) 1124, 1125
BcuI ACTAGT 2 cut(s) 157, 1883
BfaI CTAG 6 cut(s) 158, 240, 719, 1430, 1658, 1884
BfmI CTRYAG 3 cut(s) 12, 816, 1584
BfoI RGCGCY 1 cut(s) 487
BglI GCCNNNNNGGC 1 cut(s) 1654
BisI GCNGC 9 cut(s) 179, 182, 198, 765, 816, 864, 948, 1094, 1692
BlsI GCNGC 9 cut(s) 180, 183, 199, 766, 817, 865, 949, 1095, 1693
Bme1390I CCNGG 3 cut(s) 519, 1124, 1125
Bme18I GGWCC 1 cut(s) 493
BmeT110I CYCGRG 2 cut(s) 1123, 1915
BmgBI CACGTC 2 cut(s) 577, 1865
BmgT120I GGNCC 1 cut(s) 493
BmiI GGNNCC 1 cut(s) 618
BmrFI CCNGG 3 cut(s) 519, 1124, 1125
BmsI GCATC 8 cut(s) 493, 691, 850, 1102, 1228, 1649, 1807, 2135
BpiI GAAGAC 4 cut(s) 579, 1379, 1514, 1689
BpmI CTGGAG 3 cut(s) 445, 1475, 1734
Bpu10I CCTNAGC 3 cut(s) 507, 951, 1492
BpuEI CTTGAG 1 cut(s) 2042
BpuMI CCSGG 2 cut(s) 1124, 1125
BsaAI YACGTR 1 cut(s) 756
BsaHI GRCGYC 1 cut(s) 649
BsaJI CCNNGG 3 cut(s) 1122, 1123, 2175
BsaWI WCCGGW 1 cut(s) 166
BsaXI ACNNNNNCTCC 2 cut(s) 1082, 1112
Bsc4I CCNNNNNNNGG 5 cut(s) 143, 1129, 1575, 1576, 2122
Bse118I RCCGGY 1 cut(s) 166
Bse1I ACTGG 4 cut(s) 69, 428, 1458, 2298
Bse21I CCTNAGG 1 cut(s) 1086
Bse3DI GCAATG 3 cut(s) 1642, 1846, 2316
BseBI CCWGG 1 cut(s) 519
BseDI CCNNGG 3 cut(s) 1122, 1123, 2175
BseGI GGATG 6 cut(s) 355, 463, 517, 1153, 1376, 1822
BseLI CCNNNNNNNGG 5 cut(s) 143, 1129, 1575, 1576, 2122
BseMI GCAATG 3 cut(s) 1642, 1846, 2316
BseNI ACTGG 4 cut(s) 69, 428, 1458, 2298
BseRI GAGGAG 1 cut(s) 1103
BseSI GKGCMC 1 cut(s) 2305
BseXI GCAGC 6 cut(s) 209, 776, 802, 850, 934, 1678
BsgI GTGCAG 1 cut(s) 1992
Bsh1236I CGCG 1 cut(s) 1212
BshFI GGCC 1 cut(s) 469
BshTI ACCGGT 1 cut(s) 166
BsiHKAI GWGCWC 2 cut(s) 1750, 2305
BsiHKCI CYCGRG 2 cut(s) 1123, 1915
BsiSI CCGG 3 cut(s) 167, 1124, 1327
BslFI GGGAC 1 cut(s) 2099
BslI CCNNNNNNNGG 5 cut(s) 143, 1129, 1575, 1576, 2122
BsmFI GGGAC 1 cut(s) 2099
BsmI GAATGC 2 cut(s) 817, 2411
BsnI GGCC 1 cut(s) 469
BsoBI CYCGRG 2 cut(s) 1123, 1915
Bsp1286I GDGCHC 4 cut(s) 55, 619, 1750, 2305
Bsp1407I TGTACA 1 cut(s) 769
Bsp143I GATC 6 cut(s) 274, 514, 1081, 1267, 1384, 1660
Bsp68I TCGCGA 1 cut(s) 1212
BspACI CCGC 8 cut(s) 44, 78, 178, 181, 550, 1093, 1775, 1850
BspANI GGCC 1 cut(s) 469
BspFNI CGCG 1 cut(s) 1212
BspLI GGNNCC 1 cut(s) 618
BspMAI CTGCAG 1 cut(s) 820
BspPI GGATC 4 cut(s) 269, 509, 1076, 1655
BspQI GCTCTTC 1 cut(s) 717
BsrBI CCGCTC 1 cut(s) 1093
BsrDI GCAATG 3 cut(s) 1642, 1846, 2316
BsrFI RCCGGY 1 cut(s) 166
BsrGI TGTACA 1 cut(s) 769
BsrI ACTGG 4 cut(s) 69, 428, 1458, 2298
BssAI RCCGGY 1 cut(s) 166
BssECI CCNNGG 3 cut(s) 1122, 1123, 2175
BssMI GATC 6 cut(s) 274, 514, 1081, 1267, 1384, 1660
BssNI GRCGYC 1 cut(s) 649
BssT1I CCWWGG 1 cut(s) 2175
Bst2UI CCWGG 1 cut(s) 519
Bst4CI ACNGT 4 cut(s) 694, 1533, 1585, 2090
Bst6I CTCTTC 2 cut(s) 243, 717
BstACI GRCGYC 1 cut(s) 649
BstAPI GCANNNNNTGC 1 cut(s) 812
BstAUI TGTACA 1 cut(s) 769
BstBAI YACGTR 1 cut(s) 756
BstC8I GCNNGC 3 cut(s) 742, 1822, 2074
BstF5I GGATG 6 cut(s) 355, 463, 517, 1153, 1376, 1822
BstFNI CGCG 1 cut(s) 1212
BstH2I RGCGCY 1 cut(s) 487
BstHHI GCGC 3 cut(s) 486, 1243, 1351
BstKTI GATC 6 cut(s) 277, 517, 1084, 1270, 1387, 1663
BstMBI GATC 6 cut(s) 274, 514, 1081, 1267, 1384, 1660
BstMWI GCNNNNNNNGC 6 cut(s) 773, 812, 944, 1247, 1488, 1654
BstNI CCWGG 1 cut(s) 519
BstNSI RCATGY 2 cut(s) 2076, 2309
BstSCI CCNGG 3 cut(s) 517, 1122, 1123
BstSFI CTRYAG 3 cut(s) 12, 816, 1584
BstSLI GKGCMC 1 cut(s) 2305
BstUI CGCG 1 cut(s) 1212
BstV1I GCAGC 6 cut(s) 209, 776, 802, 850, 934, 1678
BstV2I GAAGAC 4 cut(s) 579, 1379, 1514, 1689
BstX2I RGATCY 1 cut(s) 1660
BstXI CCANNNNNNTGG 2 cut(s) 423, 2293
BstYI RGATCY 1 cut(s) 1660
Bsu36I CCTNAGG 1 cut(s) 1086
BsuRI GGCC 1 cut(s) 469
BtrI CACGTC 2 cut(s) 577, 1865
BtsCI GGATG 6 cut(s) 355, 463, 517, 1153, 1376, 1822
BtsIMutI CAGTG 4 cut(s) 76, 228, 637, 806
BtuMI TCGCGA 1 cut(s) 1212
Cac8I GCNNGC 3 cut(s) 742, 1822, 2074
CaiI CAGNNNCTG 2 cut(s) 506, 698
CfoI GCGC 3 cut(s) 486, 1243, 1351
Cfr10I RCCGGY 1 cut(s) 166
Cfr13I GGNCC 1 cut(s) 493
Cfr9I CCCGGG 1 cut(s) 1123
CseI GACGC 2 cut(s) 96, 1598
Csp6I GTAC 1 cut(s) 770
CspAI ACCGGT 1 cut(s) 166
CviAII CATG 8 cut(s) 397, 964, 1036, 2073, 2223, 2306, 2410, 2423
CviQI GTAC 1 cut(s) 770
DpnI GATC 6 cut(s) 276, 516, 1083, 1269, 1386, 1662
DpnII GATC 6 cut(s) 274, 514, 1081, 1267, 1384, 1660
DraI TTTAAA 2 cut(s) 1191, 2202
DrdI GACNNNNNNGTC 1 cut(s) 2383
DseDI GACNNNNNNGTC 1 cut(s) 2383
Eam1104I CTCTTC 2 cut(s) 243, 717
EarI CTCTTC 2 cut(s) 243, 717
EciI GGCGGA 1 cut(s) 565
Eco130I CCWWGG 1 cut(s) 2175
Eco147I AGGCCT 1 cut(s) 469
Eco24I GRGCYC 2 cut(s) 55, 619
Eco32I GATATC 2 cut(s) 463, 628
Eco47I GGWCC 1 cut(s) 493
Eco47III AGCGCT 1 cut(s) 485
Eco57I CTGAAG 2 cut(s) 204, 1010
Eco81I CCTNAGG 1 cut(s) 1086
Eco88I CYCGRG 2 cut(s) 1123, 1915
EcoRII CCWGG 1 cut(s) 517
EcoRV GATATC 2 cut(s) 463, 628
EcoT14I CCWWGG 1 cut(s) 2175
EcoT22I ATGCAT 4 cut(s) 1117, 1642, 2078, 2411
EcoT38I GRGCYC 2 cut(s) 55, 619
ErhI CCWWGG 1 cut(s) 2175
FaeI CATG 8 cut(s) 400, 967, 1039, 2076, 2226, 2309, 2413, 2426
FalI AAGNNNNNCTT 4 cut(s) 1329, 1361, 1792, 1824
FaqI GGGAC 1 cut(s) 2099
FatI CATG 8 cut(s) 396, 963, 1035, 2072, 2222, 2305, 2409, 2422
FauI CCCGC 2 cut(s) 51, 1768
FauNDI CATATG 2 cut(s) 778, 1500
FbaI TGATCA 1 cut(s) 1267
FblI GTMKAC 2 cut(s) 1587, 2159
Fnu4HI GCNGC 9 cut(s) 179, 182, 198, 765, 816, 864, 948, 1094, 1692
FokI GGATG 6 cut(s) 362, 470, 524, 1160, 1363, 1829
FriOI GRGCYC 2 cut(s) 55, 619
Fsp4HI GCNGC 9 cut(s) 179, 182, 198, 765, 816, 864, 948, 1094, 1692
FspBI CTAG 6 cut(s) 158, 240, 719, 1430, 1658, 1884
FspI TGCGCA 1 cut(s) 1350
GlaI GCGC 3 cut(s) 485, 1242, 1350
GluI GCNGC 9 cut(s) 179, 182, 198, 765, 816, 864, 948, 1094, 1692
GsuI CTGGAG 3 cut(s) 445, 1475, 1734
HaeII RGCGCY 1 cut(s) 487
HaeIII GGCC 1 cut(s) 469
HapII CCGG 3 cut(s) 167, 1124, 1327
HgaI GACGC 2 cut(s) 96, 1598
HhaI GCGC 3 cut(s) 486, 1243, 1351
Hin1I GRCGYC 1 cut(s) 649
Hin1II CATG 8 cut(s) 400, 967, 1039, 2076, 2226, 2309, 2413, 2426
Hin6I GCGC 3 cut(s) 484, 1241, 1349
HinP1I GCGC 3 cut(s) 484, 1241, 1349
HincII GTYRAC 2 cut(s) 403, 2160
HindII GTYRAC 2 cut(s) 403, 2160
HindIII AAGCTT 4 cut(s) 216, 380, 936, 1301
HpaII CCGG 3 cut(s) 167, 1124, 1327
HphI GGTGA 3 cut(s) 221, 323, 1591
Hpy166II GTNNAC 5 cut(s) 403, 1588, 2160, 2266, 2303
Hpy188III TCNNGA 9 cut(s) 719, 833, 1085, 1211, 1227, 1720, 1964, 1974, 2059
Hpy8I GTNNAC 5 cut(s) 403, 1588, 2160, 2266, 2303
Hpy99I CGWCG 3 cut(s) 654, 1212, 1578
HpyCH4III ACNGT 4 cut(s) 694, 1533, 1585, 2090
HpyCH4IV ACGT 6 cut(s) 576, 649, 755, 1519, 1573, 1864
HpyF10VI GCNNNNNNNGC 6 cut(s) 773, 812, 944, 1247, 1488, 1654
HpySE526I ACGT 6 cut(s) 576, 649, 755, 1519, 1573, 1864
Hsp92I GRCGYC 1 cut(s) 649
Hsp92II CATG 8 cut(s) 400, 967, 1039, 2076, 2226, 2309, 2413, 2426
HspAI GCGC 3 cut(s) 484, 1241, 1349
Ksp22I TGATCA 1 cut(s) 1267
Kzo9I GATC 6 cut(s) 274, 514, 1081, 1267, 1384, 1660
LguI GCTCTTC 1 cut(s) 717
LmnI GCTCC 5 cut(s) 622, 1090, 1329, 1753, 1836
Lsp1109I GCAGC 6 cut(s) 209, 776, 802, 850, 934, 1678
LweI GCATC 8 cut(s) 493, 691, 850, 1102, 1228, 1649, 1807, 2135
MaeI CTAG 6 cut(s) 158, 240, 719, 1430, 1658, 1884
MaeII ACGT 6 cut(s) 576, 649, 755, 1519, 1573, 1864
MaeIII GTNAC 5 cut(s) 71, 153, 632, 1006, 1579
MalI GATC 6 cut(s) 276, 516, 1083, 1269, 1386, 1662
MbiI CCGCTC 1 cut(s) 1093
MboI GATC 6 cut(s) 274, 514, 1081, 1267, 1384, 1660
MfeI CAATTG 2 cut(s) 1074, 2240
MflI RGATCY 1 cut(s) 1660
MhlI GDGCHC 4 cut(s) 55, 619, 1750, 2305
MlyI GAGTC 4 cut(s) 710, 839, 902, 1213
MmeI TCCRAC 3 cut(s) 49, 268, 440
Mph1103I ATGCAT 4 cut(s) 1117, 1642, 2078, 2411
MroXI GAANNNNTTC 2 cut(s) 1521, 2327
MseI TTAA 7 cut(s) 162, 480, 600, 1059, 1190, 1989, 2201
MslI CAYNNNNRTG 1 cut(s) 1002
MspA1I CMGCKG 3 cut(s) 200, 767, 947
MspI CCGG 3 cut(s) 167, 1124, 1327
MspR9I CCNGG 3 cut(s) 519, 1124, 1125
MunI CAATTG 2 cut(s) 1074, 2240
Mva1269I GAATGC 2 cut(s) 817, 2411
MvaI CCWGG 1 cut(s) 519
MvnI CGCG 1 cut(s) 1212
MwoI GCNNNNNNNGC 6 cut(s) 773, 812, 944, 1247, 1488, 1654
NciI CCSGG 2 cut(s) 1124, 1125
NdeI CATATG 2 cut(s) 778, 1500
NdeII GATC 6 cut(s) 274, 514, 1081, 1267, 1384, 1660
NlaIII CATG 8 cut(s) 400, 967, 1039, 2076, 2226, 2309, 2413, 2426
NlaIV GGNNCC 1 cut(s) 618
NmuCI GTSAC 3 cut(s) 71, 632, 1579
NruI TCGCGA 1 cut(s) 1212
NsbI TGCGCA 1 cut(s) 1350
NsiI ATGCAT 4 cut(s) 1117, 1642, 2078, 2411
NspI RCATGY 2 cut(s) 2076, 2309
PaeI GCATGC 1 cut(s) 2076
PaeR7I CTCGAG 1 cut(s) 1915
PceI AGGCCT 1 cut(s) 469
PciSI GCTCTTC 1 cut(s) 717
PcsI WCGNNNNNNNCGW 1 cut(s) 965
PctI GAATGC 2 cut(s) 817, 2411
PdmI GAANNNNTTC 2 cut(s) 1521, 2327
PfoI TCCNGGA 1 cut(s) 517
PinAI ACCGGT 1 cut(s) 166
PkrI GCNGC 9 cut(s) 180, 183, 199, 766, 817, 865, 949, 1095, 1693
PleI GAGTC 4 cut(s) 709, 839, 902, 1212
PpsI GAGTC 4 cut(s) 709, 839, 902, 1212
Ppu21I YACGTR 1 cut(s) 756
PsiI TTATAA 2 cut(s) 1544, 2094
Psp6I CCWGG 1 cut(s) 517
PspGI CCWGG 1 cut(s) 517
PspN4I GGNNCC 1 cut(s) 618
PspPI GGNCC 1 cut(s) 493
PspXI VCTCGAGB 1 cut(s) 1915
PstI CTGCAG 1 cut(s) 820
PstNI CAGNNNCTG 2 cut(s) 506, 698
PsuI RGATCY 1 cut(s) 1660
PvuII CAGCTG 3 cut(s) 200, 767, 947
RruI TCGCGA 1 cut(s) 1212
RsaI GTAC 1 cut(s) 771
RsaNI GTAC 1 cut(s) 770
RseI CAYNNNNRTG 1 cut(s) 1002
SalI GTCGAC 1 cut(s) 2158
SapI GCTCTTC 1 cut(s) 717
SaqAI TTAA 7 cut(s) 162, 480, 600, 1059, 1190, 1989, 2201
SatI GCNGC 9 cut(s) 179, 182, 198, 765, 816, 864, 948, 1094, 1692
Sau3AI GATC 6 cut(s) 274, 514, 1081, 1267, 1384, 1660
Sau96I GGNCC 1 cut(s) 493
SchI GAGTC 4 cut(s) 710, 839, 902, 1213
ScrFI CCNGG 3 cut(s) 519, 1124, 1125
SduI GDGCHC 4 cut(s) 55, 619, 1750, 2305
SfaNI GCATC 8 cut(s) 493, 691, 850, 1102, 1228, 1649, 1807, 2135
SfcI CTRYAG 3 cut(s) 12, 816, 1584
Sfr274I CTCGAG 1 cut(s) 1915
SinI GGWCC 1 cut(s) 493
SlaI CTCGAG 1 cut(s) 1915
SmaI CCCGGG 1 cut(s) 1125
SmiMI CAYNNNNRTG 1 cut(s) 1002
SmlI CTYRAG 2 cut(s) 1915, 2057
SmoI CTYRAG 2 cut(s) 1915, 2057
SpeI ACTAGT 2 cut(s) 157, 1883
SphI GCATGC 1 cut(s) 2076
SseBI AGGCCT 1 cut(s) 469
SsiI CCGC 8 cut(s) 44, 78, 178, 181, 550, 1093, 1775, 1850
SspI AATATT 1 cut(s) 2344
SspMI CTAG 6 cut(s) 158, 240, 719, 1430, 1658, 1884
StuI AGGCCT 1 cut(s) 469
StyD4I CCNGG 3 cut(s) 517, 1122, 1123
StyI CCWWGG 1 cut(s) 2175
TaaI ACNGT 4 cut(s) 694, 1533, 1585, 2090
TaiI ACGT 6 cut(s) 579, 652, 758, 1522, 1576, 1867
TaqI TCGA 8 cut(s) 906, 959, 1226, 1916, 2159, 2282, 2375, 2460
TatI WGTACW 1 cut(s) 769
TauI GCSGC 3 cut(s) 181, 184, 1096
Tru1I TTAA 7 cut(s) 162, 480, 600, 1059, 1190, 1989, 2201
Tru9I TTAA 7 cut(s) 162, 480, 600, 1059, 1190, 1989, 2201
TscAI CASTG 4 cut(s) 76, 228, 637, 813
TseFI GTSAC 3 cut(s) 71, 632, 1579
TseI GCWGC 6 cut(s) 197, 764, 815, 863, 947, 1691
Tsp45I GTSAC 3 cut(s) 71, 632, 1579
TspMI CCCGGG 1 cut(s) 1123
TspRI CASTG 4 cut(s) 76, 228, 637, 813
VneI GTGCAC 1 cut(s) 2301
VpaK11BI GGWCC 1 cut(s) 493
XapI RAATTY 5 cut(s) 671, 827, 1047, 1894, 2046
XbaI TCTAGA 1 cut(s) 718
XceI RCATGY 2 cut(s) 2076, 2309
XhoI CTCGAG 1 cut(s) 1915
XmaI CCCGGG 1 cut(s) 1123
XmiI GTMKAC 2 cut(s) 1587, 2159
XmnI GAANNNNTTC 2 cut(s) 1521, 2327
XspI CTAG 6 cut(s) 158, 240, 719, 1430, 1658, 1884
ZraI GACGTC 1 cut(s) 650
Zsp2I ATGCAT 4 cut(s) 1117, 1642, 2078, 2411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.