Rroxscaffold_3G00220190

Vacuolar sorting 38 and autophagy-related subunit 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009921
Physical Location & Seq
Reverse (-)
2401626 .. 2407611
5986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00220190.1

Sequence Viewer

Length: 1437 bp
ATGACGAACAAGAAATCCAGTAATTGTGCAATTTGTGAGAATTCGAATCTCGCATCCATCTGTGCTGTCTGTGTCAATTACAGATTGAATGATTACAACAATTCTTTGAAGGCACTAAAGAGTCGTCGCGATTCCTTGTATTCGAGATTGAGTGATGCGCTTGTCGCAAAGGGTAAGGCAGATGATCAACTAAATTGGAGAGTGCTTCAAAATGAGAAGCTCGTAAGGTTGAAGGAAAAGCTCCGGCGTAATAAAGAACAACTTGCGCAAGGGAAGGCTAAGATTGAGAAGACATCCTATGATCTAAAAGTCAAGTATGGGGTGCTTGAATCAGCCCGCTCTATGCTAGAAAAAAATCGTGCTGAACAACTGGAGAAGTTCTATCCTAATCTTATATGCACTCAGAGCTTAGGGCATATGGCAATTACATCTGAACGTCTTCATAAACAGTCGGTGGTTATAAAACAAATATGCAAGTTGTTTCCCCAACGTCGGGTGACTGTAGACGCAAAAAGGAAAGACGGTTCTGGTGGTCAATATGACCAGATTTGCAATGCATCGTTGCCAAGAGGGCTAGATCCACATTCAGTTCCCTCAGAAGATCTTGCTGCTTCTTTGGGATATATGGTTCAGCTTTTGAATCTTGTTGTTCAGAACTTAGGTGCTCCAGCCCTTCATAACTCAGGTTTTGCGGGTTCTTGCTCTCGTATATGGCAAAGAGATTCTTATTGGGATGCACGCCCTTCTTCTCGGAGCAATGAATATCCGCTATTTATTCCACGTCAAAATTATTGCTCTACTAGTGGGGAAAATTCGTGGTCTGATAGAAGCTCGAGTAATTTTGGTGTTGCTTCAATAGAATCAGAGAGGAAACCCCGTCTGGATTCTTCTGGAAGTAGTAGCTTTAATTATTCTTCTGCTTCTGCACATTCTGTTGAAACCCACAAAGATTTGCAGAGAGGAATTTCACTTCTCAAGAAAAGTGTGGCATGCATTACAGCATACTGTTATAACTCACTATGTTTAGATGTCCCTTCTGAGGCATCTACCTTTGAAGCATTTGCAAAGTTGCTGTCGACCTTATCTTCTTCCAAGGAAGTCCATTCAGTTTTTTATTTAAAAATGGCTTGTTCAAGGTCATGTAAGCAAGTTCAACAATTGAACAAATCTGTATGGAATGTGAACTCTGCCATAACTTCGACCACTCTACTGGATAGTGCACATCCAATGACAATGACGAAAAACTTCTGTGAAAATAATATTCCTAATTATGCCACCAGCTTCCTTTCTTCGACTGAGATGTCTGATTCTGGGAAGAATGAATGCATGATTGAAGGGTGGGATCTTGTGGAACATCCGACTCTTCCTCCACCATCCCAATCTGAAGATATTGAGCATTGGACTCGAGCCATGATTATTGATGCAACAAAGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atg14

Protein Analysis

478

Amino Acids

53.38

Weight (kDa)

8.91

Isoelectric Point (pI)

55.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG14 PF10186 9 - 366 5.6e-92 Vacuolar sorting 38 and autophagy-related subunit 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 461, 1011
AasI GACNNNNNNGTC 1 cut(s) 1300
Acc16I TGCGCA 1 cut(s) 267
AccBSI CCGCTC 1 cut(s) 339
AccI GTMKAC 2 cut(s) 504, 1076
AccII CGCG 1 cut(s) 129
AciI CCGC 3 cut(s) 337, 692, 767
AclWI GGATC 2 cut(s) 572, 1350
AcsI RAATTY 3 cut(s) 40, 811, 963
AcuI CTGAAG 1 cut(s) 1404
AfiI CCNNNNNNNGG 3 cut(s) 492, 493, 1039
AhlI ACTAGT 1 cut(s) 800
AjiI CACGTC 1 cut(s) 782
AloI GAACNNNNNNTCC 5 cut(s) 31, 508, 540, 612, 644
AluBI AGCT 7 cut(s) 220, 241, 408, 634, 831, 903, 1281
AluI AGCT 7 cut(s) 220, 241, 408, 634, 831, 903, 1281
Alw21I GWGCWC 2 cut(s) 667, 1222
Alw44I GTGCAC 1 cut(s) 1218
AlwI GGATC 2 cut(s) 572, 1350
Ama87I CYCGRG 2 cut(s) 832, 1404
ApaLI GTGCAC 1 cut(s) 1218
ApeKI GCWGC 1 cut(s) 608
ApoI RAATTY 3 cut(s) 40, 811, 963
ArsI GACNNNNNNTTYG 2 cut(s) 1058, 1090
Asp700I GAANNNNTTC 2 cut(s) 438, 1244
AspLEI GCGC 2 cut(s) 160, 268
AsuHPI GGTGA 1 cut(s) 508
AsuII TTCGAA 1 cut(s) 44
AvaI CYCGRG 2 cut(s) 832, 1404
BaeGI GKGCMC 1 cut(s) 1222
BbsI GAAGAC 2 cut(s) 296, 431
Bbv12I GWGCWC 2 cut(s) 667, 1222
BbvI GCAGC 1 cut(s) 595
BccI CCATC 2 cut(s) 65, 1381
BcgI CGANNNNNNTGC 2 cut(s) 1385, 1419
BclI TGATCA 1 cut(s) 184
BcuI ACTAGT 1 cut(s) 800
BfaI CTAG 3 cut(s) 347, 575, 801
BfmI CTRYAG 1 cut(s) 501
BglI GCCNNNNNGGC 1 cut(s) 571
BglII AGATCT 1 cut(s) 601
BisI GCNGC 1 cut(s) 609
BlsI GCNGC 1 cut(s) 610
BmeT110I CYCGRG 2 cut(s) 832, 1404
BmgBI CACGTC 1 cut(s) 782
BmsI GCATC 6 cut(s) 62, 145, 566, 724, 1052, 1411
BpiI GAAGAC 2 cut(s) 296, 431
BpmI CTGGAG 2 cut(s) 392, 651
Bpu10I CCTNAGC 1 cut(s) 409
Bpu14I TTCGAA 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 959
BsaJI CCNNGG 1 cut(s) 1092
BsaXI ACNNNNNCTCC 2 cut(s) 1351, 1381
Bsc4I CCNNNNNNNGG 3 cut(s) 492, 493, 1039
Bse1I ACTGG 3 cut(s) 18, 375, 1215
Bse3DI GCAATG 2 cut(s) 559, 763
BseDI CCNNGG 1 cut(s) 1092
BseGI GGATG 6 cut(s) 53, 293, 739, 1222, 1354, 1373
BseLI CCNNNNNNNGG 3 cut(s) 492, 493, 1039
BseMI GCAATG 2 cut(s) 559, 763
BseMII CTCAG 5 cut(s) 416, 609, 696, 1029, 1287
BseNI ACTGG 3 cut(s) 18, 375, 1215
BseSI GKGCMC 1 cut(s) 1222
BseXI GCAGC 1 cut(s) 595
BsgI GTGCAG 1 cut(s) 909
Bsh1236I CGCG 1 cut(s) 129
BsiHKAI GWGCWC 2 cut(s) 667, 1222
BsiHKCI CYCGRG 2 cut(s) 832, 1404
BsiSI CCGG 1 cut(s) 244
BslFI GGGAC 1 cut(s) 1016
BslI CCNNNNNNNGG 3 cut(s) 492, 493, 1039
BsmFI GGGAC 1 cut(s) 1016
BsmI GAATGC 1 cut(s) 1328
BsoBI CYCGRG 2 cut(s) 832, 1404
Bsp119I TTCGAA 1 cut(s) 44
Bsp1286I GDGCHC 2 cut(s) 667, 1222
Bsp143I GATC 5 cut(s) 184, 301, 577, 601, 1342
Bsp68I TCGCGA 1 cut(s) 129
BspACI CCGC 3 cut(s) 337, 692, 767
BspCNI CTCAG 5 cut(s) 415, 608, 695, 1030, 1288
BspFNI CGCG 1 cut(s) 129
BspPI GGATC 2 cut(s) 572, 1350
BspT104I TTCGAA 1 cut(s) 44
BsrBI CCGCTC 1 cut(s) 339
BsrDI GCAATG 2 cut(s) 559, 763
BsrI ACTGG 3 cut(s) 18, 375, 1215
BssECI CCNNGG 1 cut(s) 1092
BssMI GATC 5 cut(s) 184, 301, 577, 601, 1342
BssT1I CCWWGG 1 cut(s) 1092
Bst4CI ACNGT 4 cut(s) 450, 502, 524, 1007
Bst6I CTCTTC 1 cut(s) 1368
BstBI TTCGAA 1 cut(s) 44
BstC8I GCNNGC 3 cut(s) 337, 739, 991
BstDEI CTNAG 8 cut(s) 279, 402, 409, 595, 658, 682, 1038, 1296
BstF5I GGATG 6 cut(s) 53, 293, 739, 1222, 1354, 1373
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 2 cut(s) 160, 268
BstKTI GATC 5 cut(s) 187, 304, 580, 604, 1345
BstMBI GATC 5 cut(s) 184, 301, 577, 601, 1342
BstMWI GCNNNNNNNGC 3 cut(s) 164, 405, 571
BstNSI RCATGY 1 cut(s) 993
BstSFI CTRYAG 1 cut(s) 501
BstSLI GKGCMC 1 cut(s) 1222
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 1 cut(s) 595
BstV2I GAAGAC 2 cut(s) 296, 431
BstX2I RGATCY 3 cut(s) 577, 601, 1342
BstXI CCANNNNNNTGG 1 cut(s) 1210
BstYI RGATCY 3 cut(s) 577, 601, 1342
BtrI CACGTC 1 cut(s) 782
BtsCI GGATG 6 cut(s) 53, 293, 739, 1222, 1354, 1373
BtuMI TCGCGA 1 cut(s) 129
Cac8I GCNNGC 3 cut(s) 337, 739, 991
CfoI GCGC 2 cut(s) 160, 268
CseI GACGC 1 cut(s) 515
CviAII CATG 5 cut(s) 990, 1140, 1327, 1411, 1434
DdeI CTNAG 8 cut(s) 279, 402, 409, 595, 658, 682, 1038, 1296
DpnI GATC 5 cut(s) 186, 303, 579, 603, 1344
DpnII GATC 5 cut(s) 184, 301, 577, 601, 1342
DraI TTTAAA 1 cut(s) 1119
DrdI GACNNNNNNGTC 1 cut(s) 1300
DseDI GACNNNNNNGTC 1 cut(s) 1300
Eam1104I CTCTTC 1 cut(s) 1368
EarI CTCTTC 1 cut(s) 1368
Eco130I CCWWGG 1 cut(s) 1092
Eco57I CTGAAG 1 cut(s) 1404
Eco88I CYCGRG 2 cut(s) 832, 1404
EcoRI GAATTC 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 1092
EcoT22I ATGCAT 3 cut(s) 559, 995, 1328
ErhI CCWWGG 1 cut(s) 1092
FaeI CATG 5 cut(s) 993, 1143, 1330, 1414, 1437
FalI AAGNNNNNCTT 4 cut(s) 246, 278, 709, 741
FaqI GGGAC 1 cut(s) 1016
FatI CATG 5 cut(s) 989, 1139, 1326, 1410, 1433
FauI CCCGC 2 cut(s) 344, 685
FauNDI CATATG 1 cut(s) 417
FbaI TGATCA 1 cut(s) 184
FblI GTMKAC 2 cut(s) 504, 1076
Fnu4HI GCNGC 1 cut(s) 609
FokI GGATG 6 cut(s) 40, 280, 746, 1209, 1341, 1360
Fsp4HI GCNGC 1 cut(s) 609
FspBI CTAG 3 cut(s) 347, 575, 801
FspI TGCGCA 1 cut(s) 267
GlaI GCGC 2 cut(s) 159, 267
GluI GCNGC 1 cut(s) 609
GsuI CTGGAG 2 cut(s) 392, 651
HapII CCGG 1 cut(s) 244
HgaI GACGC 1 cut(s) 515
HhaI GCGC 2 cut(s) 160, 268
Hin1II CATG 5 cut(s) 993, 1143, 1330, 1414, 1437
Hin6I GCGC 2 cut(s) 158, 266
HinP1I GCGC 2 cut(s) 158, 266
HincII GTYRAC 1 cut(s) 1077
HindII GTYRAC 1 cut(s) 1077
HpaII CCGG 1 cut(s) 244
HphI GGTGA 1 cut(s) 508
Hpy166II GTNNAC 4 cut(s) 505, 1077, 1183, 1220
Hpy188III TCNNGA 5 cut(s) 128, 144, 881, 891, 976
Hpy8I GTNNAC 4 cut(s) 505, 1077, 1183, 1220
Hpy99I CGWCG 2 cut(s) 129, 495
HpyAV CCTTC 7 cut(s) 103, 226, 268, 683, 753, 1044, 1328
HpyCH4III ACNGT 4 cut(s) 450, 502, 524, 1007
HpyCH4IV ACGT 3 cut(s) 436, 490, 781
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 405, 571
HpyF3I CTNAG 8 cut(s) 279, 402, 409, 595, 658, 682, 1038, 1296
HpySE526I ACGT 3 cut(s) 436, 490, 781
Hsp92II CATG 5 cut(s) 993, 1143, 1330, 1414, 1437
HspAI GCGC 2 cut(s) 158, 266
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 5 cut(s) 184, 301, 577, 601, 1342
LmnI GCTCC 3 cut(s) 246, 670, 753
Lsp1109I GCAGC 1 cut(s) 595
LweI GCATC 6 cut(s) 62, 145, 566, 724, 1052, 1411
MaeI CTAG 3 cut(s) 347, 575, 801
MaeII ACGT 3 cut(s) 436, 490, 781
MaeIII GTNAC 1 cut(s) 496
MalI GATC 5 cut(s) 186, 303, 579, 603, 1344
MbiI CCGCTC 1 cut(s) 339
MboI GATC 5 cut(s) 184, 301, 577, 601, 1342
MfeI CAATTG 1 cut(s) 1157
MflI RGATCY 3 cut(s) 577, 601, 1342
MhlI GDGCHC 2 cut(s) 667, 1222
MlyI GAGTC 3 cut(s) 130, 1354, 1396
MmeI TCCRAC 1 cut(s) 1382
MnlI CCTC 6 cut(s) 563, 604, 861, 953, 1033, 1377
Mph1103I ATGCAT 3 cut(s) 559, 995, 1328
MroXI GAANNNNTTC 2 cut(s) 438, 1244
MseI TTAA 2 cut(s) 906, 1118
MspI CCGG 1 cut(s) 244
MunI CAATTG 1 cut(s) 1157
Mva1269I GAATGC 1 cut(s) 1328
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 3 cut(s) 164, 405, 571
NdeI CATATG 1 cut(s) 417
NdeII GATC 5 cut(s) 184, 301, 577, 601, 1342
NlaIII CATG 5 cut(s) 993, 1143, 1330, 1414, 1437
NmuCI GTSAC 1 cut(s) 496
NruI TCGCGA 1 cut(s) 129
NsbI TGCGCA 1 cut(s) 267
NsiI ATGCAT 3 cut(s) 559, 995, 1328
NspI RCATGY 1 cut(s) 993
NspV TTCGAA 1 cut(s) 44
PaeI GCATGC 1 cut(s) 993
PaeR7I CTCGAG 2 cut(s) 832, 1404
PctI GAATGC 1 cut(s) 1328
PdmI GAANNNNTTC 2 cut(s) 438, 1244
PfeI GAWTC 8 cut(s) 46, 131, 329, 640, 722, 860, 884, 1307
PkrI GCNGC 1 cut(s) 610
PleI GAGTC 3 cut(s) 129, 1354, 1396
PpsI GAGTC 3 cut(s) 129, 1354, 1396
PsiI TTATAA 2 cut(s) 461, 1011
PspXI VCTCGAGB 2 cut(s) 832, 1404
PsuI RGATCY 3 cut(s) 577, 601, 1342
RruI TCGCGA 1 cut(s) 129
SalI GTCGAC 1 cut(s) 1075
SaqAI TTAA 2 cut(s) 906, 1118
SatI GCNGC 1 cut(s) 609
Sau3AI GATC 5 cut(s) 184, 301, 577, 601, 1342
SchI GAGTC 3 cut(s) 130, 1354, 1396
SduI GDGCHC 2 cut(s) 667, 1222
SfaNI GCATC 6 cut(s) 62, 145, 566, 724, 1052, 1411
SfcI CTRYAG 1 cut(s) 501
Sfr274I CTCGAG 2 cut(s) 832, 1404
SfuI TTCGAA 1 cut(s) 44
SlaI CTCGAG 2 cut(s) 832, 1404
SmlI CTYRAG 3 cut(s) 832, 974, 1404
SmoI CTYRAG 3 cut(s) 832, 974, 1404
SpeI ACTAGT 1 cut(s) 800
SphI GCATGC 1 cut(s) 993
SsiI CCGC 3 cut(s) 337, 692, 767
SspI AATATT 1 cut(s) 1261
SspMI CTAG 3 cut(s) 347, 575, 801
StyI CCWWGG 1 cut(s) 1092
TaaI ACNGT 4 cut(s) 450, 502, 524, 1007
TaiI ACGT 3 cut(s) 439, 493, 784
TaqI TCGA 7 cut(s) 44, 143, 833, 1076, 1199, 1292, 1405
TfiI GAWTC 8 cut(s) 46, 131, 329, 640, 722, 860, 884, 1307
Tru1I TTAA 2 cut(s) 906, 1118
Tru9I TTAA 2 cut(s) 906, 1118
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 1 cut(s) 608
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 4 cut(s) 431, 665, 774, 1335
VneI GTGCAC 1 cut(s) 1218
XapI RAATTY 3 cut(s) 40, 811, 963
XceI RCATGY 1 cut(s) 993
XhoI CTCGAG 2 cut(s) 832, 1404
XmiI GTMKAC 2 cut(s) 504, 1076
XmnI GAANNNNTTC 2 cut(s) 438, 1244
XspI CTAG 3 cut(s) 347, 575, 801
Zsp2I ATGCAT 3 cut(s) 559, 995, 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.