Rh7BG462400

Vacuolar sorting 38 and autophagy-related subunit 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0009921
Physical Location & Seq
Forward (+)
54805023 .. 54811204
6182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG462400.1

Sequence Viewer

Length: 1518 bp
ATGACGAACAAGAAATCCAGTAATTGTGCAATTTGTGAGAATTCGAACCTCGCATCCATCTGTGCTGTCTGTGTCAATTACAGATTGAATGATTACAACAATTCTTTGAAGGCACTAAAGAGTCGTCGCGATTCCTTGTATTCGAGATTGAGTGATGCGCTTGTCGCAAAGGGTAAGGCAGATGATCAACTAAATTGGAGAGTGCTTCAAAATGAGAAGCTGGTGAGGTTGAGGGAAAAGCTCCGGCGTAATAAAGAACAACTTGCGCAAGGGAAGGCTAAAATTGAGAAGACATCCTATGATCTAAAAGTCAAGAATGGGGTGCTTGAATCAGCCCTCTCTATGGTACAACACAGTAATCTCGTATTTCATTCCATTATTCAATCTTTGAGATATATGCTGACTTCTAACTTTTTGTATTTGCAGCTAGAAAAAAATCGTGCTGAACAACTGGAGAAGTTCTATCCTAATCTTATATGCACTCAGAGCTTAGGGCATATGGCAATTACATCTGAACGTCTTCATAAACAGTCGGTGGTTATAAAACAAATATGCAAGTTGTTTCCCCAACGTCGGGTGACTGTAGACGCAAAGAGGAAAGAGGGCTCTGGTGGTCAATATGACCAGATTTGCAATGCATCGTTGCCAAGAGGGCTAGATCCACACTCAGTTCCCTCAGAAGACCTTGCTGCTTCTTTGGGATATATGGTTCAACTCTTGAATCTTGTTGTTCAGAACTTAGGTGCTCCAGCCCTTCATAACTCAGGTTTTGCGGGTTCTTGCTCTCGTATATGGCAAAGAGATTCTTATTGGGATGCACGCCCTTCTTCTCGGAGCAATGAATATCCGCTATTTATTCCACGTCAAAATTATTGCTCTACTAGTGGGGAAAATTCGTGGTCTGATAGAAGCTCGAGTAATTTTGGTGTTGCTTCAATAGAATCAGAGAGGAAACCCCGTCTGGATTCTTCTGGAAGTAGTAGCTTTAATTATTCTTCTGCTTCTGCACATTCTGTTGAAACCCACAAAGATTTGCAGAGAGGAATTTCACTTCTCAAGAAAAGTGTGGCATGCATTACAGCATACTGTTATAACTCACTATGTTTAGATGTCCCTTCGGAGGCATCTACCTTTGAAGCATTTGCAAAGTTGCTGTCGACCTTATCTTCTTCCAAGGAAGTCCATTCAGTTTTTTATTTAAAAATGGCTTGTTCAAGGTCATGTAAGCAAGTTCAACAATTGAACAAATCTGTATGGGATGTGAACTCTGCCATATCTTCGACCACTCTACTGGATAGTGCACATGCAATGACAATGACGAAAAACTTCTGTGAAAATAATATTCCTAATTATGCCACCAGCTTCCTTTCTTCGACTGAGATGTCTGATTCTGGGAAGAATGAATGCATGATTGAGGGGTGGGATCTTGTGGAACATCCGACTCTTCCTCCACCATCCCAATCTGAAGATATTGAGCATTGGACTCGAGCGATGTTTATTGATGCAACAAAGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atg14

Protein Analysis

505

Amino Acids

56.56

Weight (kDa)

8.85

Isoelectric Point (pI)

55.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG14 PF10186 9 - 393 4.8e-85 Vacuolar sorting 38 and autophagy-related subunit 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 542, 1092
AasI GACNNNNNNGTC 1 cut(s) 1381
Acc16I TGCGCA 1 cut(s) 267
AccI GTMKAC 2 cut(s) 585, 1157
AccII CGCG 1 cut(s) 129
AciI CCGC 2 cut(s) 773, 848
AclWI GGATC 2 cut(s) 653, 1431
AcsI RAATTY 3 cut(s) 40, 892, 1044
AcuI CTGAAG 1 cut(s) 1485
AfaI GTAC 1 cut(s) 348
AfiI CCNNNNNNNGG 4 cut(s) 343, 573, 574, 1120
AhlI ACTAGT 1 cut(s) 881
AjiI CACGTC 1 cut(s) 863
AloI GAACNNNNNNTCC 5 cut(s) 31, 38, 70, 693, 725
AluBI AGCT 7 cut(s) 220, 241, 427, 489, 912, 984, 1362
AluI AGCT 7 cut(s) 220, 241, 427, 489, 912, 984, 1362
Alw21I GWGCWC 2 cut(s) 748, 1303
Alw44I GTGCAC 1 cut(s) 1299
AlwI GGATC 2 cut(s) 653, 1431
Ama87I CYCGRG 2 cut(s) 913, 1485
ApaLI GTGCAC 1 cut(s) 1299
ApeKI GCWGC 2 cut(s) 424, 689
ApoI RAATTY 3 cut(s) 40, 892, 1044
ArsI GACNNNNNNTTYG 2 cut(s) 1139, 1171
Asp700I GAANNNNTTC 2 cut(s) 519, 1325
AspLEI GCGC 2 cut(s) 160, 268
AsuHPI GGTGA 2 cut(s) 235, 589
AsuII TTCGAA 1 cut(s) 44
AvaI CYCGRG 2 cut(s) 913, 1485
BaeGI GKGCMC 1 cut(s) 1303
BanII GRGCYC 1 cut(s) 608
BbsI GAAGAC 3 cut(s) 296, 512, 687
Bbv12I GWGCWC 2 cut(s) 748, 1303
BbvI GCAGC 2 cut(s) 436, 676
BccI CCATC 2 cut(s) 65, 1462
BcgI CGANNNNNNTGC 2 cut(s) 1466, 1500
BclI TGATCA 1 cut(s) 184
BcuI ACTAGT 1 cut(s) 881
BfaI CTAG 3 cut(s) 428, 656, 882
BfmI CTRYAG 1 cut(s) 582
BglI GCCNNNNNGGC 1 cut(s) 652
BisI GCNGC 2 cut(s) 425, 690
BlsI GCNGC 2 cut(s) 426, 691
BmeT110I CYCGRG 2 cut(s) 913, 1485
BmgBI CACGTC 1 cut(s) 863
BmsI GCATC 6 cut(s) 62, 145, 647, 805, 1133, 1492
BpiI GAAGAC 3 cut(s) 296, 512, 687
BpmI CTGGAG 2 cut(s) 473, 732
Bpu10I CCTNAGC 1 cut(s) 490
Bpu14I TTCGAA 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 1040
BsaJI CCNNGG 1 cut(s) 1173
BsaXI ACNNNNNCTCC 2 cut(s) 1432, 1462
Bsc4I CCNNNNNNNGG 4 cut(s) 343, 573, 574, 1120
Bse1I ACTGG 3 cut(s) 18, 456, 1296
Bse3DI GCAATG 3 cut(s) 640, 844, 1314
BseDI CCNNGG 1 cut(s) 1173
BseGI GGATG 6 cut(s) 53, 293, 820, 1264, 1435, 1454
BseLI CCNNNNNNNGG 4 cut(s) 343, 573, 574, 1120
BseMI GCAATG 3 cut(s) 640, 844, 1314
BseMII CTCAG 5 cut(s) 497, 681, 690, 777, 1368
BseNI ACTGG 3 cut(s) 18, 456, 1296
BseSI GKGCMC 1 cut(s) 1303
BseXI GCAGC 2 cut(s) 436, 676
BsgI GTGCAG 1 cut(s) 990
Bsh1236I CGCG 1 cut(s) 129
BsiHKAI GWGCWC 2 cut(s) 748, 1303
BsiHKCI CYCGRG 2 cut(s) 913, 1485
BsiSI CCGG 1 cut(s) 244
BslFI GGGAC 1 cut(s) 1097
BslI CCNNNNNNNGG 4 cut(s) 343, 573, 574, 1120
BsmFI GGGAC 1 cut(s) 1097
BsmI GAATGC 1 cut(s) 1409
BsoBI CYCGRG 2 cut(s) 913, 1485
Bsp119I TTCGAA 1 cut(s) 44
Bsp1286I GDGCHC 3 cut(s) 608, 748, 1303
Bsp143I GATC 4 cut(s) 184, 301, 658, 1423
Bsp68I TCGCGA 1 cut(s) 129
BspACI CCGC 2 cut(s) 773, 848
BspCNI CTCAG 5 cut(s) 496, 680, 689, 776, 1369
BspFNI CGCG 1 cut(s) 129
BspPI GGATC 2 cut(s) 653, 1431
BspT104I TTCGAA 1 cut(s) 44
BsrDI GCAATG 3 cut(s) 640, 844, 1314
BsrI ACTGG 3 cut(s) 18, 456, 1296
BssECI CCNNGG 1 cut(s) 1173
BssMI GATC 4 cut(s) 184, 301, 658, 1423
BssT1I CCWWGG 1 cut(s) 1173
Bst4CI ACNGT 4 cut(s) 356, 531, 583, 1088
Bst6I CTCTTC 1 cut(s) 1449
BstBI TTCGAA 1 cut(s) 44
BstC8I GCNNGC 2 cut(s) 820, 1072
BstDEI CTNAG 7 cut(s) 483, 490, 667, 676, 739, 763, 1377
BstF5I GGATG 6 cut(s) 53, 293, 820, 1264, 1435, 1454
BstFNI CGCG 1 cut(s) 129
BstHHI GCGC 2 cut(s) 160, 268
BstKTI GATC 4 cut(s) 187, 304, 661, 1426
BstMBI GATC 4 cut(s) 184, 301, 658, 1423
BstMWI GCNNNNNNNGC 3 cut(s) 164, 486, 652
BstNSI RCATGY 2 cut(s) 1074, 1307
BstSFI CTRYAG 1 cut(s) 582
BstSLI GKGCMC 1 cut(s) 1303
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 2 cut(s) 436, 676
BstV2I GAAGAC 3 cut(s) 296, 512, 687
BstX2I RGATCY 2 cut(s) 658, 1423
BstXI CCANNNNNNTGG 1 cut(s) 1291
BstYI RGATCY 2 cut(s) 658, 1423
BtgZI GCGATG 1 cut(s) 1505
BtrI CACGTC 1 cut(s) 863
BtsCI GGATG 6 cut(s) 53, 293, 820, 1264, 1435, 1454
BtuMI TCGCGA 1 cut(s) 129
Cac8I GCNNGC 2 cut(s) 820, 1072
CfoI GCGC 2 cut(s) 160, 268
CseI GACGC 1 cut(s) 596
Csp6I GTAC 1 cut(s) 347
CviAII CATG 5 cut(s) 1071, 1221, 1304, 1408, 1515
CviQI GTAC 1 cut(s) 347
DdeI CTNAG 7 cut(s) 483, 490, 667, 676, 739, 763, 1377
DpnI GATC 4 cut(s) 186, 303, 660, 1425
DpnII GATC 4 cut(s) 184, 301, 658, 1423
DraI TTTAAA 1 cut(s) 1200
DrdI GACNNNNNNGTC 1 cut(s) 1381
DseDI GACNNNNNNGTC 1 cut(s) 1381
Eam1104I CTCTTC 1 cut(s) 1449
EarI CTCTTC 1 cut(s) 1449
Eco130I CCWWGG 1 cut(s) 1173
Eco24I GRGCYC 1 cut(s) 608
Eco57I CTGAAG 1 cut(s) 1485
Eco88I CYCGRG 2 cut(s) 913, 1485
EcoRI GAATTC 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 1173
EcoT22I ATGCAT 3 cut(s) 640, 1076, 1409
EcoT38I GRGCYC 1 cut(s) 608
ErhI CCWWGG 1 cut(s) 1173
FaeI CATG 5 cut(s) 1074, 1224, 1307, 1411, 1518
FalI AAGNNNNNCTT 4 cut(s) 246, 278, 790, 822
FaqI GGGAC 1 cut(s) 1097
FatI CATG 5 cut(s) 1070, 1220, 1303, 1407, 1514
FauI CCCGC 1 cut(s) 766
FauNDI CATATG 1 cut(s) 498
FbaI TGATCA 1 cut(s) 184
FblI GTMKAC 2 cut(s) 585, 1157
Fnu4HI GCNGC 2 cut(s) 425, 690
FokI GGATG 6 cut(s) 40, 280, 827, 1271, 1422, 1441
FriOI GRGCYC 1 cut(s) 608
Fsp4HI GCNGC 2 cut(s) 425, 690
FspBI CTAG 3 cut(s) 428, 656, 882
FspI TGCGCA 1 cut(s) 267
GlaI GCGC 2 cut(s) 159, 267
GluI GCNGC 2 cut(s) 425, 690
GsuI CTGGAG 2 cut(s) 473, 732
HapII CCGG 1 cut(s) 244
HgaI GACGC 1 cut(s) 596
HhaI GCGC 2 cut(s) 160, 268
Hin1II CATG 5 cut(s) 1074, 1224, 1307, 1411, 1518
Hin6I GCGC 2 cut(s) 158, 266
HinP1I GCGC 2 cut(s) 158, 266
HincII GTYRAC 1 cut(s) 1158
HindII GTYRAC 1 cut(s) 1158
HpaII CCGG 1 cut(s) 244
HphI GGTGA 2 cut(s) 235, 589
Hpy166II GTNNAC 4 cut(s) 586, 1158, 1264, 1301
Hpy188III TCNNGA 7 cut(s) 128, 144, 313, 718, 962, 972, 1057
Hpy8I GTNNAC 4 cut(s) 586, 1158, 1264, 1301
Hpy99I CGWCG 2 cut(s) 129, 576
HpyAV CCTTC 5 cut(s) 103, 268, 764, 834, 1125
HpyCH4III ACNGT 4 cut(s) 356, 531, 583, 1088
HpyCH4IV ACGT 3 cut(s) 517, 571, 862
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 486, 652
HpyF3I CTNAG 7 cut(s) 483, 490, 667, 676, 739, 763, 1377
HpySE526I ACGT 3 cut(s) 517, 571, 862
Hsp92II CATG 5 cut(s) 1074, 1224, 1307, 1411, 1518
HspAI GCGC 2 cut(s) 158, 266
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 4 cut(s) 184, 301, 658, 1423
LmnI GCTCC 3 cut(s) 246, 751, 834
Lsp1109I GCAGC 2 cut(s) 436, 676
LweI GCATC 6 cut(s) 62, 145, 647, 805, 1133, 1492
MaeI CTAG 3 cut(s) 428, 656, 882
MaeII ACGT 3 cut(s) 517, 571, 862
MaeIII GTNAC 1 cut(s) 577
MalI GATC 4 cut(s) 186, 303, 660, 1425
MboI GATC 4 cut(s) 184, 301, 658, 1423
MfeI CAATTG 1 cut(s) 1238
MflI RGATCY 2 cut(s) 658, 1423
MhlI GDGCHC 3 cut(s) 608, 748, 1303
MlyI GAGTC 3 cut(s) 130, 1435, 1477
MmeI TCCRAC 1 cut(s) 1463
Mph1103I ATGCAT 3 cut(s) 640, 1076, 1409
MroXI GAANNNNTTC 2 cut(s) 519, 1325
MseI TTAA 2 cut(s) 987, 1199
MspI CCGG 1 cut(s) 244
MunI CAATTG 1 cut(s) 1238
Mva1269I GAATGC 1 cut(s) 1409
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 3 cut(s) 164, 486, 652
NdeI CATATG 1 cut(s) 498
NdeII GATC 4 cut(s) 184, 301, 658, 1423
NlaIII CATG 5 cut(s) 1074, 1224, 1307, 1411, 1518
NmuCI GTSAC 1 cut(s) 577
NruI TCGCGA 1 cut(s) 129
NsbI TGCGCA 1 cut(s) 267
NsiI ATGCAT 3 cut(s) 640, 1076, 1409
NspI RCATGY 2 cut(s) 1074, 1307
NspV TTCGAA 1 cut(s) 44
PaeI GCATGC 1 cut(s) 1074
PaeR7I CTCGAG 2 cut(s) 913, 1485
PctI GAATGC 1 cut(s) 1409
PdmI GAANNNNTTC 2 cut(s) 519, 1325
PfeI GAWTC 7 cut(s) 131, 329, 721, 803, 941, 965, 1388
PkrI GCNGC 2 cut(s) 426, 691
PleI GAGTC 3 cut(s) 129, 1435, 1477
PpsI GAGTC 3 cut(s) 129, 1435, 1477
PsiI TTATAA 2 cut(s) 542, 1092
PspXI VCTCGAGB 2 cut(s) 913, 1485
PsuI RGATCY 2 cut(s) 658, 1423
RruI TCGCGA 1 cut(s) 129
RsaI GTAC 1 cut(s) 348
RsaNI GTAC 1 cut(s) 347
SalI GTCGAC 1 cut(s) 1156
SaqAI TTAA 2 cut(s) 987, 1199
SatI GCNGC 2 cut(s) 425, 690
Sau3AI GATC 4 cut(s) 184, 301, 658, 1423
SchI GAGTC 3 cut(s) 130, 1435, 1477
SduI GDGCHC 3 cut(s) 608, 748, 1303
SfaNI GCATC 6 cut(s) 62, 145, 647, 805, 1133, 1492
SfcI CTRYAG 1 cut(s) 582
Sfr274I CTCGAG 2 cut(s) 913, 1485
SfuI TTCGAA 1 cut(s) 44
SlaI CTCGAG 2 cut(s) 913, 1485
SmlI CTYRAG 3 cut(s) 913, 1055, 1485
SmoI CTYRAG 3 cut(s) 913, 1055, 1485
SpeI ACTAGT 1 cut(s) 881
SphI GCATGC 1 cut(s) 1074
SsiI CCGC 2 cut(s) 773, 848
SspI AATATT 1 cut(s) 1342
SspMI CTAG 3 cut(s) 428, 656, 882
StyI CCWWGG 1 cut(s) 1173
TaaI ACNGT 4 cut(s) 356, 531, 583, 1088
TaiI ACGT 3 cut(s) 520, 574, 865
TaqI TCGA 7 cut(s) 44, 143, 914, 1157, 1280, 1373, 1486
TfiI GAWTC 7 cut(s) 131, 329, 721, 803, 941, 965, 1388
Tru1I TTAA 2 cut(s) 987, 1199
Tru9I TTAA 2 cut(s) 987, 1199
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 2 cut(s) 424, 689
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 5 cut(s) 359, 512, 746, 855, 1416
VneI GTGCAC 1 cut(s) 1299
XapI RAATTY 3 cut(s) 40, 892, 1044
XceI RCATGY 2 cut(s) 1074, 1307
XhoI CTCGAG 2 cut(s) 913, 1485
XmiI GTMKAC 2 cut(s) 585, 1157
XmnI GAANNNNTTC 2 cut(s) 519, 1325
XspI CTAG 3 cut(s) 428, 656, 882
Zsp2I ATGCAT 3 cut(s) 640, 1076, 1409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.