FvH4_5g38340

Vacuolar sorting 38 and autophagy-related subunit 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009921
Physical Location & Seq
Forward (+)
28351934 .. 28358077
6144 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g38340.t1

Sequence Viewer

Length: 1437 bp
ATGACGAACAAGAAGTCCAGTAATTGCGCAATTTGTGAGAATTCGAATCTCGCATCTATCTGTGCTGTTTGTGTCAATTACAGATTAAATGATTACAACAATTCGTTAAAGGCGCTAAAGAGTCGTCGCGATTTGTTGTATTCAAGATTGAGTGATGCACTTGTTGCTAAGGGTAAGGCAGATGATCAACTAAATTGGAGAATACTTCAAGATGAAAAGCTCGTGAGGTTGAGGGAAAAGCTCCGTCGTAATAAAGAACAACTTGTGCAAGGGAAGGCTAAGATTGAGAAGACATCATATGACCTAAAAGTCAAATATGGGGTGCTTGAGTCGGCCCTCTCTATGTTGGAAAAAAATCGTGCTGAACAACTGGAGAAGTTCTATCCTAATCTTATATGCACTCAGAGCTTAGGGCATATGGCAATTACTTCTGAACGTCTTCATAAACAGTCGGTGGTTATAAAACAAATATGCAAATTGTTTCCTCAACGTCGGGTGACTGTAGACGCAAAGAGGAAAGAGGGATCTGGTGGTCAATATGACCAGATTTGCAATGCATCGTTGCCAAGAGGGCTAGATCCACACTCAGTTCCCTCAGAAGAGCTTGCTGCTTCTTTGGGATATATGGTTCAACTCTTGAACCTTGTTGTCCAGAACTTAGGTGCTCCAGCCCTTCATAACTCAGGTTTTGCGGGTTCTTGCTCTCGTATATGGCAAAGAGATTCTTATTGGGATGCACGCCCTTCTTCTCGGAGCAATGAATATCCGCTATTTATTCCACGGCAAAATTATTGCTCTACTAGTGGAGAAAATTCGTGGTCTGACAGAAGCTCAAGTAATTTTGGTGTTGCTTCAATAGAATCAGAGAGGAAACCCCGTCTGGATTCCTCTGGAAGTAGTAGCTTTAATTATTCTTCCGCTTCTCAACATTCTGTGGAAACCCACAAAGATTTGCAGAGAGGAATTTCACTTCTCAAGAAAAGTGTGGCATGCATTACAGCATACTGTTATAACTCACTATGTTTAGATGTCCCTTCCGAGGCATCTACCTTTGAAGCATTTGCAAAGTTGCTGTCAACCTTATCTTCTTCCAAGGAAGTCCATTCGGTTTTTTCCTTAAAAATGGCTTGTTCAAGGTCATGTAAGCAAGTTCAACAATTGAACAAATCTGTATGGAATGTGAACTCTGCCATTTCTTCGACCACTCTACTGGATAGTGCGCATACAATGACAATGACGAAAAACTTTTATGAAAATAATATTCCTAATTATGCAACGAGCTTCCTTTCTTCGACTGAGATGTCTGATGTTGGGAAGAATGAATGCACGATTGAAGGGTGGGATCTTGTGGAACATCCAACTCTTCCTCCACCATCCCAATCTGAAGATATTGAGCATTGGACTCGAGCGATGTTTATTGATGTAACAAAGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atg14

Protein Analysis

479

Amino Acids

53.59

Weight (kDa)

8.89

Isoelectric Point (pI)

56.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG14 PF10186 9 - 366 3.3e-89 Vacuolar sorting 38 and autophagy-related subunit 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 461, 1011
AasI GACNNNNNNGTC 2 cut(s) 308, 1300
Acc16I TGCGCA 2 cut(s) 28, 1221
AccI GTMKAC 1 cut(s) 504
AccII CGCG 1 cut(s) 129
AciI CCGC 3 cut(s) 692, 767, 918
AclWI GGATC 3 cut(s) 532, 572, 1350
AcsI RAATTY 3 cut(s) 40, 811, 963
AcuI CTGAAG 1 cut(s) 1404
AfiI CCNNNNNNNGG 1 cut(s) 1039
AhlI ACTAGT 1 cut(s) 800
AloI GAACNNNNNNTCC 3 cut(s) 31, 612, 644
AluBI AGCT 7 cut(s) 220, 241, 408, 604, 831, 903, 1281
AluI AGCT 7 cut(s) 220, 241, 408, 604, 831, 903, 1281
Alw21I GWGCWC 1 cut(s) 667
AlwI GGATC 3 cut(s) 532, 572, 1350
Ama87I CYCGRG 1 cut(s) 1404
AoxI GGCC 1 cut(s) 333
ApeKI GCWGC 1 cut(s) 608
ApoI RAATTY 3 cut(s) 40, 811, 963
ArsI GACNNNNNNTTYG 3 cut(s) 31, 1058, 1090
Asp700I GAANNNNTTC 1 cut(s) 438
AspLEI GCGC 3 cut(s) 29, 115, 1222
AspS9I GGNCC 1 cut(s) 334
AsuHPI GGTGA 1 cut(s) 508
AsuII TTCGAA 1 cut(s) 44
AvaI CYCGRG 1 cut(s) 1404
BauI CACGAG 1 cut(s) 221
BbsI GAAGAC 2 cut(s) 296, 431
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 1 cut(s) 595
BccI CCATC 1 cut(s) 1381
BceAI ACGGC 1 cut(s) 797
BcgI CGANNNNNNTGC 2 cut(s) 1385, 1419
BclI TGATCA 1 cut(s) 184
BcuI ACTAGT 1 cut(s) 800
BfaI CTAG 2 cut(s) 575, 801
BfmI CTRYAG 1 cut(s) 501
BfoI RGCGCY 1 cut(s) 116
BglI GCCNNNNNGGC 1 cut(s) 571
BisI GCNGC 1 cut(s) 609
BlsI GCNGC 1 cut(s) 610
BmeT110I CYCGRG 1 cut(s) 1404
BmgT120I GGNCC 1 cut(s) 334
BmsI GCATC 5 cut(s) 62, 145, 566, 724, 1052
BpiI GAAGAC 2 cut(s) 296, 431
BpmI CTGGAG 2 cut(s) 392, 651
Bpu10I CCTNAGC 2 cut(s) 168, 409
Bpu14I TTCGAA 1 cut(s) 44
BpuEI CTTGAG 3 cut(s) 347, 817, 959
BsaJI CCNNGG 3 cut(s) 779, 1038, 1092
BsaXI ACNNNNNCTCC 2 cut(s) 1351, 1381
Bsc4I CCNNNNNNNGG 1 cut(s) 1039
Bse1I ACTGG 3 cut(s) 18, 375, 1215
Bse3DI GCAATG 2 cut(s) 559, 763
BseDI CCNNGG 3 cut(s) 779, 1038, 1092
BseGI GGATG 3 cut(s) 739, 1354, 1373
BseLI CCNNNNNNNGG 1 cut(s) 1039
BseMI GCAATG 2 cut(s) 559, 763
BseMII CTCAG 5 cut(s) 416, 600, 609, 696, 1287
BseNI ACTGG 3 cut(s) 18, 375, 1215
BseXI GCAGC 1 cut(s) 595
Bsh1236I CGCG 1 cut(s) 129
BshFI GGCC 1 cut(s) 335
BsiHKAI GWGCWC 1 cut(s) 667
BsiHKCI CYCGRG 1 cut(s) 1404
BslFI GGGAC 1 cut(s) 1016
BslI CCNNNNNNNGG 1 cut(s) 1039
BsmFI GGGAC 1 cut(s) 1016
BsmI GAATGC 1 cut(s) 1328
BsnI GGCC 1 cut(s) 335
BsoBI CYCGRG 1 cut(s) 1404
Bsp119I TTCGAA 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 667
Bsp143I GATC 4 cut(s) 184, 524, 577, 1342
Bsp68I TCGCGA 1 cut(s) 129
BspACI CCGC 3 cut(s) 692, 767, 918
BspANI GGCC 1 cut(s) 335
BspCNI CTCAG 5 cut(s) 415, 599, 608, 695, 1288
BspFNI CGCG 1 cut(s) 129
BspPI GGATC 3 cut(s) 532, 572, 1350
BspQI GCTCTTC 1 cut(s) 594
BspT104I TTCGAA 1 cut(s) 44
BsrDI GCAATG 2 cut(s) 559, 763
BsrI ACTGG 3 cut(s) 18, 375, 1215
BssECI CCNNGG 3 cut(s) 779, 1038, 1092
BssMI GATC 4 cut(s) 184, 524, 577, 1342
BssSI CACGAG 1 cut(s) 221
BssT1I CCWWGG 1 cut(s) 1092
Bst2BI CACGAG 1 cut(s) 221
Bst4CI ACNGT 3 cut(s) 450, 502, 1007
Bst6I CTCTTC 2 cut(s) 594, 1368
BstAPI GCANNNNNTGC 1 cut(s) 164
BstBI TTCGAA 1 cut(s) 44
BstC8I GCNNGC 3 cut(s) 606, 739, 991
BstDEI CTNAG 9 cut(s) 168, 279, 402, 409, 586, 595, 658, 682, 1296
BstDSI CCRYGG 1 cut(s) 779
BstF5I GGATG 3 cut(s) 739, 1354, 1373
BstFNI CGCG 1 cut(s) 129
BstH2I RGCGCY 1 cut(s) 116
BstHHI GCGC 3 cut(s) 29, 115, 1222
BstKTI GATC 4 cut(s) 187, 527, 580, 1345
BstMBI GATC 4 cut(s) 184, 524, 577, 1342
BstMWI GCNNNNNNNGC 3 cut(s) 164, 405, 571
BstNSI RCATGY 1 cut(s) 993
BstSFI CTRYAG 1 cut(s) 501
BstUI CGCG 1 cut(s) 129
BstV1I GCAGC 1 cut(s) 595
BstV2I GAAGAC 2 cut(s) 296, 431
BstX2I RGATCY 3 cut(s) 524, 577, 1342
BstXI CCANNNNNNTGG 1 cut(s) 1210
BstYI RGATCY 3 cut(s) 524, 577, 1342
BsuRI GGCC 1 cut(s) 335
BtgI CCRYGG 1 cut(s) 779
BtgZI GCGATG 1 cut(s) 1424
BtsCI GGATG 3 cut(s) 739, 1354, 1373
BtuMI TCGCGA 1 cut(s) 129
Cac8I GCNNGC 3 cut(s) 606, 739, 991
CfoI GCGC 3 cut(s) 29, 115, 1222
Cfr13I GGNCC 1 cut(s) 334
CseI GACGC 1 cut(s) 515
CviAII CATG 2 cut(s) 990, 1140
DdeI CTNAG 9 cut(s) 168, 279, 402, 409, 586, 595, 658, 682, 1296
DpnI GATC 4 cut(s) 186, 526, 579, 1344
DpnII GATC 4 cut(s) 184, 524, 577, 1342
DrdI GACNNNNNNGTC 2 cut(s) 308, 1300
DseDI GACNNNNNNGTC 2 cut(s) 308, 1300
Eam1104I CTCTTC 2 cut(s) 594, 1368
EarI CTCTTC 2 cut(s) 594, 1368
Eco130I CCWWGG 1 cut(s) 1092
Eco57I CTGAAG 1 cut(s) 1404
Eco88I CYCGRG 1 cut(s) 1404
EcoRI GAATTC 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 1092
EcoT22I ATGCAT 2 cut(s) 559, 995
ErhI CCWWGG 1 cut(s) 1092
FaeI CATG 2 cut(s) 993, 1143
FalI AAGNNNNNCTT 4 cut(s) 246, 278, 709, 741
FaqI GGGAC 1 cut(s) 1016
FatI CATG 2 cut(s) 989, 1139
FauI CCCGC 1 cut(s) 685
FauNDI CATATG 2 cut(s) 298, 417
FbaI TGATCA 1 cut(s) 184
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 1 cut(s) 609
FokI GGATG 3 cut(s) 746, 1341, 1360
Fsp4HI GCNGC 1 cut(s) 609
FspAI RTGCGCAY 1 cut(s) 1221
FspBI CTAG 2 cut(s) 575, 801
FspI TGCGCA 2 cut(s) 28, 1221
GlaI GCGC 3 cut(s) 28, 114, 1221
GluI GCNGC 1 cut(s) 609
GsuI CTGGAG 2 cut(s) 392, 651
HaeII RGCGCY 1 cut(s) 116
HaeIII GGCC 1 cut(s) 335
HgaI GACGC 1 cut(s) 515
HhaI GCGC 3 cut(s) 29, 115, 1222
Hin1II CATG 2 cut(s) 993, 1143
Hin6I GCGC 3 cut(s) 27, 113, 1220
HinP1I GCGC 3 cut(s) 27, 113, 1220
HincII GTYRAC 1 cut(s) 1077
HindII GTYRAC 1 cut(s) 1077
HinfI GANTC 7 cut(s) 46, 121, 329, 722, 860, 884, 1402
HphI GGTGA 1 cut(s) 508
Hpy166II GTNNAC 3 cut(s) 505, 1077, 1183
Hpy188I TCNGA 9 cut(s) 405, 433, 598, 753, 823, 865, 1039, 1306, 1384
Hpy188III TCNNGA 9 cut(s) 128, 144, 209, 223, 637, 652, 881, 891, 976
Hpy8I GTNNAC 3 cut(s) 505, 1077, 1183
Hpy99I CGWCG 3 cut(s) 129, 249, 495
HpyAV CCTTC 5 cut(s) 268, 683, 753, 1044, 1328
HpyCH4III ACNGT 3 cut(s) 450, 502, 1007
HpyCH4IV ACGT 2 cut(s) 436, 490
HpyF10VI GCNNNNNNNGC 3 cut(s) 164, 405, 571
HpyF3I CTNAG 9 cut(s) 168, 279, 402, 409, 586, 595, 658, 682, 1296
HpySE526I ACGT 2 cut(s) 436, 490
Hsp92II CATG 2 cut(s) 993, 1143
HspAI GCGC 3 cut(s) 27, 113, 1220
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 4 cut(s) 184, 524, 577, 1342
LguI GCTCTTC 1 cut(s) 594
LmnI GCTCC 3 cut(s) 246, 670, 753
Lsp1109I GCAGC 1 cut(s) 595
LweI GCATC 5 cut(s) 62, 145, 566, 724, 1052
MaeI CTAG 2 cut(s) 575, 801
MaeII ACGT 2 cut(s) 436, 490
MaeIII GTNAC 2 cut(s) 496, 1423
MalI GATC 4 cut(s) 186, 526, 579, 1344
MboI GATC 4 cut(s) 184, 524, 577, 1342
MfeI CAATTG 1 cut(s) 1157
MflI RGATCY 3 cut(s) 524, 577, 1342
MhlI GDGCHC 1 cut(s) 667
MlyI GAGTC 3 cut(s) 130, 338, 1396
MmeI TCCRAC 2 cut(s) 327, 1382
Mph1103I ATGCAT 2 cut(s) 559, 995
MroXI GAANNNNTTC 1 cut(s) 438
MseI TTAA 4 cut(s) 86, 107, 906, 1118
MunI CAATTG 1 cut(s) 1157
Mva1269I GAATGC 1 cut(s) 1328
MvnI CGCG 1 cut(s) 129
MwoI GCNNNNNNNGC 3 cut(s) 164, 405, 571
NdeI CATATG 2 cut(s) 298, 417
NdeII GATC 4 cut(s) 184, 524, 577, 1342
NlaIII CATG 2 cut(s) 993, 1143
NmuCI GTSAC 1 cut(s) 496
NruI TCGCGA 1 cut(s) 129
NsbI TGCGCA 2 cut(s) 28, 1221
NsiI ATGCAT 2 cut(s) 559, 995
NspI RCATGY 1 cut(s) 993
NspV TTCGAA 1 cut(s) 44
PaeI GCATGC 1 cut(s) 993
PaeR7I CTCGAG 1 cut(s) 1404
PciSI GCTCTTC 1 cut(s) 594
PctI GAATGC 1 cut(s) 1328
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 4 cut(s) 46, 722, 860, 884
PkrI GCNGC 1 cut(s) 610
PleI GAGTC 3 cut(s) 129, 337, 1396
PpsI GAGTC 3 cut(s) 129, 337, 1396
PsiI TTATAA 2 cut(s) 461, 1011
PspPI GGNCC 1 cut(s) 334
PspXI VCTCGAGB 1 cut(s) 1404
PsuI RGATCY 3 cut(s) 524, 577, 1342
RruI TCGCGA 1 cut(s) 129
SapI GCTCTTC 1 cut(s) 594
SaqAI TTAA 4 cut(s) 86, 107, 906, 1118
SatI GCNGC 1 cut(s) 609
Sau3AI GATC 4 cut(s) 184, 524, 577, 1342
Sau96I GGNCC 1 cut(s) 334
SchI GAGTC 3 cut(s) 130, 338, 1396
SduI GDGCHC 1 cut(s) 667
SfaNI GCATC 5 cut(s) 62, 145, 566, 724, 1052
SfcI CTRYAG 1 cut(s) 501
Sfr274I CTCGAG 1 cut(s) 1404
SfuI TTCGAA 1 cut(s) 44
SlaI CTCGAG 1 cut(s) 1404
SmlI CTYRAG 4 cut(s) 326, 832, 974, 1404
SmoI CTYRAG 4 cut(s) 326, 832, 974, 1404
SpeI ACTAGT 1 cut(s) 800
SphI GCATGC 1 cut(s) 993
SsiI CCGC 3 cut(s) 692, 767, 918
SspI AATATT 1 cut(s) 1261
SspMI CTAG 2 cut(s) 575, 801
StyI CCWWGG 1 cut(s) 1092
TaaI ACNGT 3 cut(s) 450, 502, 1007
TaiI ACGT 2 cut(s) 439, 493
TaqI TCGA 4 cut(s) 44, 1199, 1292, 1405
TfiI GAWTC 4 cut(s) 46, 722, 860, 884
Tru1I TTAA 4 cut(s) 86, 107, 906, 1118
Tru9I TTAA 4 cut(s) 86, 107, 906, 1118
TseFI GTSAC 1 cut(s) 496
TseI GCWGC 1 cut(s) 608
Tsp45I GTSAC 1 cut(s) 496
TspDTI ATGAA 6 cut(s) 228, 431, 665, 774, 1266, 1335
TspGWI ACGGA 1 cut(s) 233
XapI RAATTY 3 cut(s) 40, 811, 963
XceI RCATGY 1 cut(s) 993
XhoI CTCGAG 1 cut(s) 1404
XmiI GTMKAC 1 cut(s) 504
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 2 cut(s) 575, 801
Zsp2I ATGCAT 2 cut(s) 559, 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.