RchiOBHm_Chr7g0240361

Vacuolar sorting 38 and autophagy-related subunit 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0009921
Physical Location & Seq
Forward (+)
66301523 .. 66307562
6040 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21543

Sequence Viewer

Length: 1287 bp
ATGCGCTTGTCGCAAAGGTTTGGTAAGGCAGATGATCAACTAAATTGGAGAGTGCTTCAAAATGAGAAGCTCGTAAGGTTGAGGGAAAAGCTCCGGCGTAATAAAGAACAACTTGCGCAAGGGAAGGCTAAGATTGAGAAGACATCCTATGATCTAAAAGTCAAGTATGGGGTGCTTGAATCAGCCCTCTCTATGCTAGAAAAAAATCGTGCTGAACAACTGGAGAAGTTCTATCCTAATCTTATATGCACTCAGAGCTTAGGGCATATGGCAATTACATCTGAACGTCTTCATAAACAGTCGGTGGTTATAAAACAAATATGCAAGTTGTTTCCCCAACGTCGGGTGACTGTAGACGCAAAGAGGAAAGACGGTTCTGGTGGTCAATATGACCAGATTTGCAATGCATCGTTGCCAAGAGGGCTAGATCCACACTCAGTTCCCTCAGAAGATCTTGCTGCTTCTTTGGGATATATGGTTCAACTCTTGAATCTTGTTGTTCAGAACTTAGGTGCTCCAGCCCTTCATAACTCAGGTTTTGCGGGTTCTTGCTCTCGTATATGGCAAAGAGATTCTTATTGGGATGCACGCCCATCTTCTCGGAGCAATGAATATCCGCTATTTATTCCACGTCAAAATTATTGCTCTACTAGTGGGGAAAATTCATGGTCTGATAGAAGCTCGAGTAATTTTGGTGTTGCTTCAATAGAATCAGAGAGGAAACCCCGTCTTGATTCTTCTGGAAGTAGTAGCTTTAATTATACTTCTGCTTCTGCACATTCTGTTGAAACCCACAAAGATTTGCAGAGAGGAATTTCACTTCTCAAGAAAAGTGTGGCATGCATTACAGCATACTGTTATAACTCACTGTGTTTAGATGTCCCTTCTGAGGCATCTACCTTTGAAGCATTTGCAAAGTTGCTGTCGACCTTATCTTCTTCCAAGGAAGTCCATTCAGTTTTTTATTTAAAAATGGCTTGTTCAAGGTCATGTAAGCAAGTTCAACAATTGAACAAATCTGTATGGAATGTGAACTCTGCCATATCTTCGACCACTCTACTGGATAGTGCACATGCAATGACAATGACGAAAAACTTCTGTGAAAATAATATTCCTAATTATGCCACCAGCTTCCTTTCTTCGACTGAGATGTCTGATTCTGGGAAGAATGAATGCATGATTGAAGGATGGGATCTTGTGGAACATCCGACTCTTCCTCCACCATCCCAATCTGAAGATATTGAGCATTGGACTCGAGCGATGTTTATTGATGCAACAAAGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Atg14

Protein Analysis

428

Amino Acids

47.98

Weight (kDa)

8.8

Isoelectric Point (pI)

59.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG14 PF10186 9 - 316 2.2e-76 Vacuolar sorting 38 and autophagy-related subunit 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 311, 861
AasI GACNNNNNNGTC 1 cut(s) 1150
Acc16I TGCGCA 1 cut(s) 117
AccI GTMKAC 2 cut(s) 354, 926
AciI CCGC 2 cut(s) 542, 617
AclWI GGATC 2 cut(s) 422, 1200
AcsI RAATTY 2 cut(s) 661, 813
AcuI CTGAAG 1 cut(s) 1254
AfiI CCNNNNNNNGG 3 cut(s) 342, 343, 889
AhlI ACTAGT 1 cut(s) 650
AjiI CACGTC 1 cut(s) 632
AloI GAACNNNNNNTCC 4 cut(s) 358, 390, 462, 494
AluBI AGCT 6 cut(s) 70, 91, 258, 681, 753, 1131
AluI AGCT 6 cut(s) 70, 91, 258, 681, 753, 1131
Alw21I GWGCWC 2 cut(s) 517, 1072
Alw44I GTGCAC 1 cut(s) 1068
AlwI GGATC 2 cut(s) 422, 1200
Ama87I CYCGRG 2 cut(s) 682, 1254
ApaLI GTGCAC 1 cut(s) 1068
ApeKI GCWGC 1 cut(s) 458
ApoI RAATTY 2 cut(s) 661, 813
ArsI GACNNNNNNTTYG 2 cut(s) 908, 940
Asp700I GAANNNNTTC 2 cut(s) 288, 1094
AspLEI GCGC 2 cut(s) 6, 118
AsuHPI GGTGA 1 cut(s) 358
AvaI CYCGRG 2 cut(s) 682, 1254
BaeGI GKGCMC 1 cut(s) 1072
BbsI GAAGAC 2 cut(s) 146, 281
Bbv12I GWGCWC 2 cut(s) 517, 1072
BbvI GCAGC 1 cut(s) 445
BccI CCATC 3 cut(s) 601, 1182, 1231
BcgI CGANNNNNNTGC 2 cut(s) 1235, 1269
BclI TGATCA 1 cut(s) 34
BcuI ACTAGT 1 cut(s) 650
BfaI CTAG 3 cut(s) 197, 425, 651
BfmI CTRYAG 1 cut(s) 351
BglI GCCNNNNNGGC 1 cut(s) 421
BglII AGATCT 1 cut(s) 451
BisI GCNGC 1 cut(s) 459
BlsI GCNGC 1 cut(s) 460
BmeT110I CYCGRG 2 cut(s) 682, 1254
BmgBI CACGTC 1 cut(s) 632
BmsI GCATC 4 cut(s) 416, 574, 902, 1261
BpiI GAAGAC 2 cut(s) 146, 281
BpmI CTGGAG 2 cut(s) 242, 501
Bpu10I CCTNAGC 1 cut(s) 259
BpuEI CTTGAG 1 cut(s) 809
BsaJI CCNNGG 1 cut(s) 942
BsaXI ACNNNNNCTCC 2 cut(s) 1201, 1231
Bsc4I CCNNNNNNNGG 3 cut(s) 342, 343, 889
Bse1I ACTGG 2 cut(s) 225, 1065
Bse3DI GCAATG 3 cut(s) 409, 613, 1083
BseDI CCNNGG 1 cut(s) 942
BseGI GGATG 5 cut(s) 143, 589, 1193, 1204, 1223
BseLI CCNNNNNNNGG 3 cut(s) 342, 343, 889
BseMI GCAATG 3 cut(s) 409, 613, 1083
BseMII CTCAG 6 cut(s) 266, 450, 459, 546, 879, 1137
BseNI ACTGG 2 cut(s) 225, 1065
BseSI GKGCMC 1 cut(s) 1072
BseXI GCAGC 1 cut(s) 445
BsgI GTGCAG 1 cut(s) 759
BsiHKAI GWGCWC 2 cut(s) 517, 1072
BsiHKCI CYCGRG 2 cut(s) 682, 1254
BsiSI CCGG 1 cut(s) 94
BslFI GGGAC 1 cut(s) 866
BslI CCNNNNNNNGG 3 cut(s) 342, 343, 889
BsmFI GGGAC 1 cut(s) 866
BsmI GAATGC 1 cut(s) 1178
BsoBI CYCGRG 2 cut(s) 682, 1254
Bsp1286I GDGCHC 2 cut(s) 517, 1072
Bsp143I GATC 5 cut(s) 34, 151, 427, 451, 1192
BspACI CCGC 2 cut(s) 542, 617
BspCNI CTCAG 6 cut(s) 265, 449, 458, 545, 880, 1138
BspPI GGATC 2 cut(s) 422, 1200
BsrDI GCAATG 3 cut(s) 409, 613, 1083
BsrI ACTGG 2 cut(s) 225, 1065
BssECI CCNNGG 1 cut(s) 942
BssMI GATC 5 cut(s) 34, 151, 427, 451, 1192
BssT1I CCWWGG 1 cut(s) 942
Bst4CI ACNGT 5 cut(s) 300, 352, 374, 857, 870
Bst6I CTCTTC 1 cut(s) 1218
BstC8I GCNNGC 2 cut(s) 589, 841
BstDEI CTNAG 9 cut(s) 129, 252, 259, 436, 445, 508, 532, 888, 1146
BstF5I GGATG 5 cut(s) 143, 589, 1193, 1204, 1223
BstHHI GCGC 2 cut(s) 6, 118
BstKTI GATC 5 cut(s) 37, 154, 430, 454, 1195
BstMBI GATC 5 cut(s) 34, 151, 427, 451, 1192
BstMWI GCNNNNNNNGC 3 cut(s) 10, 255, 421
BstNSI RCATGY 2 cut(s) 843, 1076
BstSFI CTRYAG 1 cut(s) 351
BstSLI GKGCMC 1 cut(s) 1072
BstV1I GCAGC 1 cut(s) 445
BstV2I GAAGAC 2 cut(s) 146, 281
BstX2I RGATCY 3 cut(s) 427, 451, 1192
BstXI CCANNNNNNTGG 1 cut(s) 1060
BstYI RGATCY 3 cut(s) 427, 451, 1192
BtgZI GCGATG 1 cut(s) 1274
BtrI CACGTC 1 cut(s) 632
BtsCI GGATG 5 cut(s) 143, 589, 1193, 1204, 1223
BtsIMutI CAGTG 1 cut(s) 866
Cac8I GCNNGC 2 cut(s) 589, 841
CfoI GCGC 2 cut(s) 6, 118
CseI GACGC 1 cut(s) 365
CviAII CATG 6 cut(s) 666, 840, 990, 1073, 1177, 1284
DdeI CTNAG 9 cut(s) 129, 252, 259, 436, 445, 508, 532, 888, 1146
DpnI GATC 5 cut(s) 36, 153, 429, 453, 1194
DpnII GATC 5 cut(s) 34, 151, 427, 451, 1192
DraI TTTAAA 1 cut(s) 969
DrdI GACNNNNNNGTC 1 cut(s) 1150
DseDI GACNNNNNNGTC 1 cut(s) 1150
Eam1104I CTCTTC 1 cut(s) 1218
EarI CTCTTC 1 cut(s) 1218
Eco130I CCWWGG 1 cut(s) 942
Eco57I CTGAAG 1 cut(s) 1254
Eco88I CYCGRG 2 cut(s) 682, 1254
EcoT14I CCWWGG 1 cut(s) 942
EcoT22I ATGCAT 3 cut(s) 409, 845, 1178
ErhI CCWWGG 1 cut(s) 942
FaeI CATG 6 cut(s) 669, 843, 993, 1076, 1180, 1287
FalI AAGNNNNNCTT 4 cut(s) 96, 128, 559, 591
FaqI GGGAC 1 cut(s) 866
FatI CATG 6 cut(s) 665, 839, 989, 1072, 1176, 1283
FauI CCCGC 1 cut(s) 535
FauNDI CATATG 1 cut(s) 267
FbaI TGATCA 1 cut(s) 34
FblI GTMKAC 2 cut(s) 354, 926
Fnu4HI GCNGC 1 cut(s) 459
FokI GGATG 5 cut(s) 130, 596, 1191, 1200, 1210
Fsp4HI GCNGC 1 cut(s) 459
FspBI CTAG 3 cut(s) 197, 425, 651
FspI TGCGCA 1 cut(s) 117
GlaI GCGC 2 cut(s) 5, 117
GluI GCNGC 1 cut(s) 459
GsuI CTGGAG 2 cut(s) 242, 501
HapII CCGG 1 cut(s) 94
HgaI GACGC 1 cut(s) 365
HhaI GCGC 2 cut(s) 6, 118
Hin1II CATG 6 cut(s) 669, 843, 993, 1076, 1180, 1287
Hin6I GCGC 2 cut(s) 4, 116
HinP1I GCGC 2 cut(s) 4, 116
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HinfI GANTC 8 cut(s) 179, 490, 572, 710, 734, 1157, 1210, 1252
HpaII CCGG 1 cut(s) 94
HphI GGTGA 1 cut(s) 358
Hpy166II GTNNAC 4 cut(s) 355, 927, 1033, 1070
Hpy188III TCNNGA 4 cut(s) 487, 731, 741, 826
Hpy8I GTNNAC 4 cut(s) 355, 927, 1033, 1070
Hpy99I CGWCG 1 cut(s) 345
HpyAV CCTTC 4 cut(s) 118, 533, 894, 1178
HpyCH4III ACNGT 5 cut(s) 300, 352, 374, 857, 870
HpyCH4IV ACGT 3 cut(s) 286, 340, 631
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 255, 421
HpyF3I CTNAG 9 cut(s) 129, 252, 259, 436, 445, 508, 532, 888, 1146
HpySE526I ACGT 3 cut(s) 286, 340, 631
Hsp92II CATG 6 cut(s) 669, 843, 993, 1076, 1180, 1287
HspAI GCGC 2 cut(s) 4, 116
Ksp22I TGATCA 1 cut(s) 34
Kzo9I GATC 5 cut(s) 34, 151, 427, 451, 1192
LmnI GCTCC 3 cut(s) 96, 520, 603
Lsp1109I GCAGC 1 cut(s) 445
LweI GCATC 4 cut(s) 416, 574, 902, 1261
MaeI CTAG 3 cut(s) 197, 425, 651
MaeII ACGT 3 cut(s) 286, 340, 631
MaeIII GTNAC 1 cut(s) 346
MalI GATC 5 cut(s) 36, 153, 429, 453, 1194
MboI GATC 5 cut(s) 34, 151, 427, 451, 1192
MfeI CAATTG 1 cut(s) 1007
MflI RGATCY 3 cut(s) 427, 451, 1192
MhlI GDGCHC 2 cut(s) 517, 1072
MluCI AATT 9 cut(s) 43, 273, 637, 661, 688, 757, 813, 1007, 1117
MlyI GAGTC 2 cut(s) 1204, 1246
MmeI TCCRAC 1 cut(s) 1232
MnlI CCTC 9 cut(s) 75, 197, 357, 413, 454, 711, 803, 883, 1227
Mph1103I ATGCAT 3 cut(s) 409, 845, 1178
MroXI GAANNNNTTC 2 cut(s) 288, 1094
MseI TTAA 2 cut(s) 756, 968
MspI CCGG 1 cut(s) 94
MunI CAATTG 1 cut(s) 1007
Mva1269I GAATGC 1 cut(s) 1178
MwoI GCNNNNNNNGC 3 cut(s) 10, 255, 421
NdeI CATATG 1 cut(s) 267
NdeII GATC 5 cut(s) 34, 151, 427, 451, 1192
NlaIII CATG 6 cut(s) 669, 843, 993, 1076, 1180, 1287
NmuCI GTSAC 1 cut(s) 346
NsbI TGCGCA 1 cut(s) 117
NsiI ATGCAT 3 cut(s) 409, 845, 1178
NspI RCATGY 2 cut(s) 843, 1076
PaeI GCATGC 1 cut(s) 843
PaeR7I CTCGAG 2 cut(s) 682, 1254
PctI GAATGC 1 cut(s) 1178
PdmI GAANNNNTTC 2 cut(s) 288, 1094
PfeI GAWTC 6 cut(s) 179, 490, 572, 710, 734, 1157
PkrI GCNGC 1 cut(s) 460
PleI GAGTC 2 cut(s) 1204, 1246
PpsI GAGTC 2 cut(s) 1204, 1246
PsiI TTATAA 2 cut(s) 311, 861
PspXI VCTCGAGB 2 cut(s) 682, 1254
PsuI RGATCY 3 cut(s) 427, 451, 1192
SalI GTCGAC 1 cut(s) 925
SaqAI TTAA 2 cut(s) 756, 968
SatI GCNGC 1 cut(s) 459
Sau3AI GATC 5 cut(s) 34, 151, 427, 451, 1192
SchI GAGTC 2 cut(s) 1204, 1246
SduI GDGCHC 2 cut(s) 517, 1072
SfaNI GCATC 4 cut(s) 416, 574, 902, 1261
SfcI CTRYAG 1 cut(s) 351
Sfr274I CTCGAG 2 cut(s) 682, 1254
SlaI CTCGAG 2 cut(s) 682, 1254
SmlI CTYRAG 3 cut(s) 682, 824, 1254
SmoI CTYRAG 3 cut(s) 682, 824, 1254
SpeI ACTAGT 1 cut(s) 650
SphI GCATGC 1 cut(s) 843
Sse9I AATT 9 cut(s) 43, 273, 637, 661, 688, 757, 813, 1007, 1117
SsiI CCGC 2 cut(s) 542, 617
SspI AATATT 1 cut(s) 1111
SspMI CTAG 3 cut(s) 197, 425, 651
StyI CCWWGG 1 cut(s) 942
TaaI ACNGT 5 cut(s) 300, 352, 374, 857, 870
TaiI ACGT 3 cut(s) 289, 343, 634
TaqI TCGA 5 cut(s) 683, 926, 1049, 1142, 1255
TasI AATT 9 cut(s) 43, 273, 637, 661, 688, 757, 813, 1007, 1117
TfiI GAWTC 6 cut(s) 179, 490, 572, 710, 734, 1157
Tru1I TTAA 2 cut(s) 756, 968
Tru9I TTAA 2 cut(s) 756, 968
TscAI CASTG 1 cut(s) 873
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 1 cut(s) 458
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 5 cut(s) 281, 515, 624, 654, 1185
TspRI CASTG 1 cut(s) 873
VneI GTGCAC 1 cut(s) 1068
XapI RAATTY 2 cut(s) 661, 813
XceI RCATGY 2 cut(s) 843, 1076
XhoI CTCGAG 2 cut(s) 682, 1254
XmiI GTMKAC 2 cut(s) 354, 926
XmnI GAANNNNTTC 2 cut(s) 288, 1094
XspI CTAG 3 cut(s) 197, 425, 651
Zsp2I ATGCAT 3 cut(s) 409, 845, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.