AT4G14450

at4g14450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010137
Physical Location & Seq
Forward (+)
8309384 .. 8310058
675 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G14450.1

Sequence Viewer

Length: 378 bp
ATGTTCTTTGATACAAAAGTACTCAATTATCCTACAATTCACAAATCTATCTCCATGGCGTCCACGATGCAAAGAACAAGCAGCTCCGCGGCCAGTAACGAGAGGCAACTTAGCCAGCTACAGAGACGAGCTCCGTCTTTAATGATCAAACCAACGTCTTTTAGTAACTGGAACGTGGCGATCCCTCTCCTTTCACCGCTCGCTCCTTCTCTAACATCATCCTTCGACCAATCACATGTTCCTCCGCCGCAGAACAAGACTGAGATACCAGTGGAAGAAGAGGTGAAGAAGACACCAGTTTTCAAGAAATGGCAACATCCCGCGTCGCCGTTCTGCTACGAGCCAACAACGTTTGTCCCACCGTTCATCCAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

14.1

Weight (kDa)

9.46

Isoelectric Point (pI)

71.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04330 AT3G23170 AT4G14450
fragaria_vesca FvH4_4g03100 FvH4_7g33480
malus_domestica MD13G1210200.v1.1 MD16G1213100.v1.1
prunus_persica Prupe.1G034700_v2.0.a1
pyrus_communis pycom13g18280
rosa_chinensis RchiOBHm_Chr4g0391391
rosa_laevigata RLG00000009867
rosa_multiflora Rmu_sc0007466.1_g000006
rosa_roxburghii Rroxscaffold_5G00337120
rosa_rugosa Rorug03G0335800
rosa_samantha Rh4AG037400 Rh4BG031600 Rh4BG031700 Rh4CG040300 Rh4CG040400 Rh4DG034900
rosa_wichuraiana Rw4G002900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 199
AccII CGCG 2 cut(s) 89, 323
AciI CCGC 6 cut(s) 87, 89, 197, 245, 248, 321
AclI AACGTT 1 cut(s) 350
AclWI GGATC 1 cut(s) 175
AcoI YGGCCR 1 cut(s) 90
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 1 cut(s) 21
AflIII ACRYGT 1 cut(s) 235
AgsI TTSAA 1 cut(s) 304
AjnI CCWGG 1 cut(s) 369
AjuI GAANNNNNNNTTGG 2 cut(s) 222, 254
AluBI AGCT 3 cut(s) 84, 118, 131
AluI AGCT 3 cut(s) 84, 118, 131
Alw21I GWGCWC 1 cut(s) 133
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 175
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 186, 295
BaeI ACNNNNGTAYC 1 cut(s) 36
BanII GRGCYC 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 1 cut(s) 93
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 1 cut(s) 371
BclI TGATCA 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 118
BfmI CTRYAG 1 cut(s) 119
BisI GCNGC 3 cut(s) 82, 90, 248
BlsI GCNGC 3 cut(s) 83, 91, 249
BmcAI AGTACT 1 cut(s) 21
Bme1390I CCNGG 1 cut(s) 371
BmrFI CCNGG 1 cut(s) 371
BmsI GCATC 1 cut(s) 57
BpiI GAAGAC 1 cut(s) 296
BplI GAGNNNNNCTC 2 cut(s) 115, 147
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 54, 87
Bse1I ACTGG 4 cut(s) 93, 173, 269, 296
BseBI CCWGG 1 cut(s) 371
BseDI CCNNGG 2 cut(s) 54, 87
BseGI GGATG 3 cut(s) 218, 316, 366
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 4 cut(s) 93, 173, 269, 296
BseXI GCAGC 1 cut(s) 93
Bsh1236I CGCG 2 cut(s) 89, 323
BshFI GGCC 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 133
BslFI GGGAC 1 cut(s) 341
BsmAI GTCTC 1 cut(s) 118
BsmBI CGTCTC 1 cut(s) 118
BsmFI GGGAC 1 cut(s) 341
BsnI GGCC 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 133
Bsp143I GATC 2 cut(s) 144, 180
Bsp19I CCATGG 1 cut(s) 54
BspACI CCGC 6 cut(s) 87, 89, 197, 245, 248, 321
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 1 cut(s) 253
BspFNI CGCG 2 cut(s) 89, 323
BspPI GGATC 1 cut(s) 175
BsrBI CCGCTC 1 cut(s) 199
BsrI ACTGG 4 cut(s) 93, 173, 269, 296
BssECI CCNNGG 2 cut(s) 54, 87
BssMI GATC 2 cut(s) 144, 180
BssNI GRCGYC 1 cut(s) 59
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 371
Bst4CI ACNGT 1 cut(s) 363
Bst6I CTCTTC 1 cut(s) 273
BstACI GRCGYC 1 cut(s) 59
BstC8I GCNNGC 2 cut(s) 116, 201
BstDEI CTNAG 2 cut(s) 110, 261
BstDSI CCRYGG 2 cut(s) 54, 87
BstF5I GGATG 3 cut(s) 218, 316, 366
BstFNI CGCG 2 cut(s) 89, 323
BstKTI GATC 2 cut(s) 147, 183
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 2 cut(s) 144, 180
BstNI CCWGG 1 cut(s) 371
BstNSI RCATGY 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 369
BstSFI CTRYAG 1 cut(s) 119
BstUI CGCG 2 cut(s) 89, 323
BstV1I GCAGC 1 cut(s) 93
BstV2I GAAGAC 1 cut(s) 296
BsuRI GGCC 1 cut(s) 92
BtgI CCRYGG 2 cut(s) 54, 87
BtsCI GGATG 3 cut(s) 218, 316, 366
BtsIMutI CAGTG 1 cut(s) 276
Cac8I GCNNGC 2 cut(s) 116, 201
Cfr42I CCGCGG 1 cut(s) 90
CseI GACGC 2 cut(s) 48, 312
Csp6I GTAC 1 cut(s) 20
CviAII CATG 2 cut(s) 55, 236
CviJI RGCY 6 cut(s) 84, 92, 114, 118, 131, 343
CviKI_1 RGCY 6 cut(s) 84, 92, 114, 118, 131, 343
CviQI GTAC 1 cut(s) 20
DdeI CTNAG 2 cut(s) 110, 261
DpnI GATC 2 cut(s) 146, 182
DpnII GATC 2 cut(s) 144, 180
EaeI YGGCCR 1 cut(s) 90
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
EciI GGCGGA 1 cut(s) 234
Ecl136II GAGCTC 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 133
Eco53kI GAGCTC 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 369
EcoT14I CCWWGG 1 cut(s) 54
EcoT38I GRGCYC 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 2 cut(s) 58, 239
FaiI YATR 2 cut(s) 56, 237
FaqI GGGAC 1 cut(s) 341
FatI CATG 2 cut(s) 54, 235
FauI CCCGC 1 cut(s) 328
FbaI TGATCA 1 cut(s) 144
Fnu4HI GCNGC 3 cut(s) 82, 90, 248
FokI GGATG 3 cut(s) 205, 303, 353
FriOI GRGCYC 1 cut(s) 133
Fsp4HI GCNGC 3 cut(s) 82, 90, 248
GluI GCNGC 3 cut(s) 82, 90, 248
HaeIII GGCC 1 cut(s) 92
HgaI GACGC 2 cut(s) 48, 312
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 2 cut(s) 58, 239
HphI GGTGA 2 cut(s) 186, 295
Hpy166II GTNNAC 1 cut(s) 63
Hpy188III TCNNGA 1 cut(s) 304
Hpy8I GTNNAC 1 cut(s) 63
Hpy99I CGWCG 1 cut(s) 328
HpyAV CCTTC 2 cut(s) 216, 232
HpyCH4III ACNGT 1 cut(s) 363
HpyCH4IV ACGT 3 cut(s) 155, 174, 350
HpyCH4V TGCA 1 cut(s) 70
HpyF3I CTNAG 2 cut(s) 110, 261
HpySE526I ACGT 3 cut(s) 155, 174, 350
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 2 cut(s) 58, 239
Ksp22I TGATCA 1 cut(s) 144
KspI CCGCGG 1 cut(s) 90
Kzo9I GATC 2 cut(s) 144, 180
LmnI GCTCC 3 cut(s) 89, 136, 208
LpnPI CCDG 6 cut(s) 106, 128, 154, 282, 309, 356
Lsp1109I GCAGC 1 cut(s) 93
LweI GCATC 1 cut(s) 57
MaeII ACGT 3 cut(s) 155, 174, 350
MaeIII GTNAC 2 cut(s) 95, 164
MalI GATC 2 cut(s) 146, 182
MbiI CCGCTC 1 cut(s) 199
MboI GATC 2 cut(s) 144, 180
MboII GAAGA 4 cut(s) 287, 290, 298, 301
MhlI GDGCHC 1 cut(s) 133
MluCI AATT 2 cut(s) 25, 36
MnlI CCTC 4 cut(s) 96, 195, 252, 274
MseI TTAA 1 cut(s) 140
MspA1I CMGCKG 1 cut(s) 89
MspR9I CCNGG 1 cut(s) 371
MvaI CCWGG 1 cut(s) 371
MvnI CGCG 2 cut(s) 89, 323
NcoI CCATGG 1 cut(s) 54
NdeII GATC 2 cut(s) 144, 180
NlaIII CATG 2 cut(s) 58, 239
NspI RCATGY 1 cut(s) 239
PciI ACATGT 1 cut(s) 235
PkrI GCNGC 3 cut(s) 83, 91, 249
PscI ACATGT 1 cut(s) 235
Psp124BI GAGCTC 1 cut(s) 133
Psp1406I AACGTT 1 cut(s) 350
Psp6I CCWGG 1 cut(s) 369
PspGI CCWGG 1 cut(s) 369
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
SacI GAGCTC 1 cut(s) 133
SacII CCGCGG 1 cut(s) 90
SaqAI TTAA 1 cut(s) 140
SatI GCNGC 3 cut(s) 82, 90, 248
Sau3AI GATC 2 cut(s) 144, 180
ScaI AGTACT 1 cut(s) 21
ScrFI CCNGG 1 cut(s) 371
SduI GDGCHC 1 cut(s) 133
SetI ASST 8 cut(s) 86, 120, 133, 158, 177, 285, 353, 375
SfaNI GCATC 1 cut(s) 57
SfcI CTRYAG 1 cut(s) 119
Sfr303I CCGCGG 1 cut(s) 90
SgrBI CCGCGG 1 cut(s) 90
Sse9I AATT 2 cut(s) 25, 36
SsiI CCGC 6 cut(s) 87, 89, 197, 245, 248, 321
SstI GAGCTC 1 cut(s) 133
StyD4I CCNGG 1 cut(s) 369
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 1 cut(s) 363
TaiI ACGT 3 cut(s) 158, 177, 353
TaqI TCGA 1 cut(s) 225
TasI AATT 2 cut(s) 25, 36
TatI WGTACW 1 cut(s) 19
TauI GCSGC 2 cut(s) 92, 250
Tru1I TTAA 1 cut(s) 140
Tru9I TTAA 1 cut(s) 140
TscAI CASTG 1 cut(s) 276
TseI GCWGC 1 cut(s) 81
TspDTI ATGAA 1 cut(s) 355
TspGWI ACGGA 1 cut(s) 123
TspRI CASTG 1 cut(s) 276
XceI RCATGY 1 cut(s) 239
ZrmI AGTACT 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.