Rroxscaffold_5G00337120

protein At4g14450, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010137
Physical Location & Seq
Reverse (-)
5157294 .. 5157650
357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00337120.1

Sequence Viewer

Length: 357 bp
ATGGCAGACATCCCCAGACCTACCTCCGGCCCCCGCCGCCAGCCCAGCCGTCTCCAGCGCCGCGCTCCTCCTCCGGCCTCTCTCCAAATAAACCCCGTCGCCGACTGGAACGTCGCCATCCCCCTCCTCTCTCCTCTCGTCTCCCCCGATCGTCCGGCCGACTCCAAACCCCGTGAAGACTCCCGCCACCACCACCAGAAACTCCAGAAACCCGACCTGCCACGTCACCAGTCCGCGGAGGCCGATAAATCCTCATCGTCGTCGTCGGCGGCGGTGGTGTTTAAGAAGTGGCAGCACCCGGCTGCCCCCTTCTGCTGCGAGCCTGCTCCGCCGGTCCGGCCTTTTGTTCCCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

118

Amino Acids

13.02

Weight (kDa)

10.27

Isoelectric Point (pI)

87.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04330 AT3G23170 AT4G14450
fragaria_vesca FvH4_4g03100 FvH4_7g33480
malus_domestica MD13G1210200.v1.1 MD16G1213100.v1.1
prunus_persica Prupe.1G034700_v2.0.a1
pyrus_communis pycom13g18280
rosa_chinensis RchiOBHm_Chr4g0391391
rosa_laevigata RLG00000009867
rosa_multiflora Rmu_sc0007466.1_g000006
rosa_roxburghii Rroxscaffold_5G00337120
rosa_rugosa Rorug03G0335800
rosa_samantha Rh4AG037400 Rh4BG031600 Rh4BG031700 Rh4CG040300 Rh4CG040400 Rh4DG034900
rosa_wichuraiana Rw4G002900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
Acc36I ACCTGC 1 cut(s) 225
AccII CGCG 2 cut(s) 63, 236
AciI CCGC 9 cut(s) 34, 37, 61, 184, 234, 236, 269, 272, 329
AcoI YGGCCR 1 cut(s) 156
AfiI CCNNNNNNNGG 2 cut(s) 26, 235
AjiI CACGTC 1 cut(s) 224
Alw26I GTCTC 2 cut(s) 56, 145
AoxI GGCC 5 cut(s) 28, 75, 156, 240, 338
ApeKI GCWGC 3 cut(s) 292, 302, 315
AspLEI GCGC 2 cut(s) 60, 65
AspS9I GGNCC 2 cut(s) 29, 334
AsuC2I CCSGG 1 cut(s) 299
AsuHPI GGTGA 1 cut(s) 218
AvaII GGWCC 1 cut(s) 334
BbsI GAAGAC 1 cut(s) 183
BbvI GCAGC 3 cut(s) 289, 302, 304
BccI CCATC 1 cut(s) 125
BceAI ACGGC 1 cut(s) 33
BcnI CCSGG 1 cut(s) 299
BcoDI GTCTC 2 cut(s) 56, 145
BfaI CTAG 1 cut(s) 355
BfoI RGCGCY 1 cut(s) 61
BfuAI ACCTGC 1 cut(s) 225
BglI GCCNNNNNGGC 1 cut(s) 337
BisI GCNGC 6 cut(s) 37, 61, 270, 293, 303, 316
BlsI GCNGC 6 cut(s) 38, 62, 271, 294, 304, 317
Bme1390I CCNGG 1 cut(s) 299
Bme18I GGWCC 1 cut(s) 334
BmgBI CACGTC 1 cut(s) 224
BmgT120I GGNCC 2 cut(s) 29, 334
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 299
BpiI GAAGAC 1 cut(s) 183
BpmI CTGGAG 2 cut(s) 38, 188
BpuMI CCSGG 1 cut(s) 299
BsaJI CCNNGG 1 cut(s) 234
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 235
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 2 cut(s) 110, 229
BseDI CCNNGG 1 cut(s) 234
BseGI GGATG 2 cut(s) 9, 117
BseLI CCNNNNNNNGG 2 cut(s) 26, 235
BseNI ACTGG 2 cut(s) 110, 229
BseRI GAGGAG 4 cut(s) 57, 60, 116, 123
BseX3I CGGCCG 1 cut(s) 156
BseXI GCAGC 3 cut(s) 289, 302, 304
BseYI CCCAGC 1 cut(s) 44
Bsh1236I CGCG 2 cut(s) 63, 236
Bsh1285I CGRYCG 2 cut(s) 151, 159
BshFI GGCC 5 cut(s) 30, 77, 158, 242, 340
BsiEI CGRYCG 2 cut(s) 151, 159
BsiSI CCGG 6 cut(s) 27, 74, 155, 299, 332, 337
BslI CCNNNNNNNGG 2 cut(s) 26, 235
BsmAI GTCTC 2 cut(s) 56, 145
BsmBI CGTCTC 2 cut(s) 56, 145
BsnI GGCC 5 cut(s) 30, 77, 158, 242, 340
Bsp143I GATC 1 cut(s) 148
BspACI CCGC 9 cut(s) 34, 37, 61, 184, 234, 236, 269, 272, 329
BspANI GGCC 5 cut(s) 30, 77, 158, 242, 340
BspFNI CGCG 2 cut(s) 63, 236
BspLI GGNNCC 1 cut(s) 31
BspMI ACCTGC 1 cut(s) 225
BsrFI RCCGGY 1 cut(s) 331
BsrI ACTGG 2 cut(s) 110, 229
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 1 cut(s) 234
BssMI GATC 1 cut(s) 148
BstC8I GCNNGC 3 cut(s) 41, 320, 324
BstDSI CCRYGG 1 cut(s) 234
BstF5I GGATG 2 cut(s) 9, 117
BstFNI CGCG 2 cut(s) 63, 236
BstH2I RGCGCY 1 cut(s) 61
BstHHI GCGC 2 cut(s) 60, 65
BstKTI GATC 1 cut(s) 151
BstMAI GTCTC 2 cut(s) 56, 145
BstMBI GATC 1 cut(s) 148
BstMCI CGRYCG 2 cut(s) 151, 159
BstMWI GCNNNNNNNGC 4 cut(s) 36, 45, 328, 337
BstSCI CCNGG 1 cut(s) 297
BstUI CGCG 2 cut(s) 63, 236
BstV1I GCAGC 3 cut(s) 289, 302, 304
BstV2I GAAGAC 1 cut(s) 183
BstZI CGGCCG 1 cut(s) 156
BsuRI GGCC 5 cut(s) 30, 77, 158, 242, 340
BtgI CCRYGG 1 cut(s) 234
BtrI CACGTC 1 cut(s) 224
BtsCI GGATG 2 cut(s) 9, 117
BveI ACCTGC 1 cut(s) 225
Cac8I GCNNGC 3 cut(s) 41, 320, 324
CfoI GCGC 2 cut(s) 60, 65
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 2 cut(s) 29, 334
Cfr42I CCGCGG 1 cut(s) 237
CpoI CGGWCCG 1 cut(s) 334
CspI CGGWCCG 1 cut(s) 334
CviJI RGCY 9 cut(s) 30, 43, 48, 77, 158, 242, 302, 322, 340
CviKI_1 RGCY 9 cut(s) 30, 43, 48, 77, 158, 242, 302, 322, 340
DpnI GATC 1 cut(s) 150
DpnII GATC 1 cut(s) 148
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 1 cut(s) 156
EagI CGGCCG 1 cut(s) 156
EciI GGCGGA 1 cut(s) 318
EclXI CGGCCG 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 334
Eco52I CGGCCG 1 cut(s) 156
Esp3I CGTCTC 2 cut(s) 56, 145
FauI CCCGC 2 cut(s) 41, 191
Fnu4HI GCNGC 6 cut(s) 37, 61, 270, 293, 303, 316
FokI GGATG 1 cut(s) 104
Fsp4HI GCNGC 6 cut(s) 37, 61, 270, 293, 303, 316
FspBI CTAG 1 cut(s) 355
GlaI GCGC 2 cut(s) 59, 64
GluI GCNGC 6 cut(s) 37, 61, 270, 293, 303, 316
GsaI CCCAGC 1 cut(s) 48
GsuI CTGGAG 2 cut(s) 38, 188
HaeII RGCGCY 1 cut(s) 61
HaeIII GGCC 5 cut(s) 30, 77, 158, 242, 340
HapII CCGG 6 cut(s) 27, 74, 155, 299, 332, 337
HhaI GCGC 2 cut(s) 60, 65
Hin6I GCGC 2 cut(s) 58, 63
HinP1I GCGC 2 cut(s) 58, 63
HinfI GANTC 2 cut(s) 161, 179
HpaII CCGG 6 cut(s) 27, 74, 155, 299, 332, 337
HphI GGTGA 1 cut(s) 218
Hpy188III TCNNGA 1 cut(s) 205
Hpy99I CGWCG 5 cut(s) 101, 116, 262, 265, 268
HpyAV CCTTC 1 cut(s) 319
HpyCH4IV ACGT 2 cut(s) 111, 223
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 45, 328, 337
HpySE526I ACGT 2 cut(s) 111, 223
HspAI GCGC 2 cut(s) 58, 63
KspI CCGCGG 1 cut(s) 237
Kzo9I GATC 1 cut(s) 148
LmnI GCTCC 2 cut(s) 70, 331
Lsp1109I GCAGC 3 cut(s) 289, 302, 304
MaeI CTAG 1 cut(s) 355
MaeII ACGT 2 cut(s) 111, 223
MaeIII GTNAC 1 cut(s) 224
MalI GATC 1 cut(s) 150
MboI GATC 1 cut(s) 148
MboII GAAGA 1 cut(s) 188
MlyI GAGTC 2 cut(s) 155, 173
MnlI CCTC 9 cut(s) 34, 78, 81, 88, 134, 137, 144, 232, 262
MseI TTAA 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 236
MspI CCGG 6 cut(s) 27, 74, 155, 299, 332, 337
MspR9I CCNGG 1 cut(s) 299
MteI GCGCNGCGC 1 cut(s) 61
MvnI CGCG 2 cut(s) 63, 236
MwoI GCNNNNNNNGC 4 cut(s) 36, 45, 328, 337
NciI CCSGG 1 cut(s) 299
NdeII GATC 1 cut(s) 148
NlaIV GGNNCC 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 224
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PkrI GCNGC 6 cut(s) 38, 62, 271, 294, 304, 317
Ple19I CGATCG 1 cut(s) 151
PleI GAGTC 2 cut(s) 155, 173
PpsI GAGTC 2 cut(s) 155, 173
PspFI CCCAGC 1 cut(s) 44
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 2 cut(s) 29, 334
PvuI CGATCG 1 cut(s) 151
Rsr2I CGGWCCG 1 cut(s) 334
RsrII CGGWCCG 1 cut(s) 334
SacII CCGCGG 1 cut(s) 237
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 6 cut(s) 37, 61, 270, 293, 303, 316
Sau3AI GATC 1 cut(s) 148
Sau96I GGNCC 2 cut(s) 29, 334
SchI GAGTC 2 cut(s) 155, 173
ScrFI CCNGG 1 cut(s) 299
SetI ASST 5 cut(s) 22, 26, 114, 219, 226
Sfr303I CCGCGG 1 cut(s) 237
SgrBI CCGCGG 1 cut(s) 237
SinI GGWCC 1 cut(s) 334
SsiI CCGC 9 cut(s) 34, 37, 61, 184, 234, 236, 269, 272, 329
SspMI CTAG 1 cut(s) 355
StyD4I CCNGG 1 cut(s) 297
TaiI ACGT 2 cut(s) 114, 226
TauI GCSGC 3 cut(s) 39, 63, 272
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TseFI GTSAC 1 cut(s) 224
TseI GCWGC 3 cut(s) 292, 302, 315
Tsp45I GTSAC 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 334
XspI CTAG 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.