RLG00000009867

protein At4g14450, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0010137
Physical Location & Seq
Forward (+)
59178247 .. 59184544
6298 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009867

Sequence Viewer

Length: 1221 bp
ATGGAATGGGCGAAATGTGTTGTGCCGTTTGTGAATTGCGGTAAAGTTAGAGTTTTCGGACATTGCATAGGTTTACCGAAAATCTTGTGTTTGATGCAAGAGATCACTTGTATTGTTTATATTCAACGTTCCGTTTACAAGGGTGTCCAGAGGTCTTTGTGGAGACTGGATGCTTCCCTTGACAATGTATCCTTTCATCACTCTGTTCAAATTGCTGAAAGTCCAACCATCTCAGAAGAGTGTTCCAGAGTATCTGCATCAGTTTTGTCAATAGAAAAACGAGGAAATGCTGGCCAACAGAATCCAGAAGAAACCAAAACCATTTCAGAGTTGTGTTTAAATGAGATTGTTGGTTCAGAAGTTGGATATAACTCGGAGGAGATGGAGCCCCCAAGTACTCTCATATGTGATCTGGTGCCATACCAGAAACAATCTCTCTATAGGATGTTGCAATTAGAGGCGGGGATTGATGTTGACAGGAGGGCTACTTCAATCTATGCGAACATGCTTCCAGGAACCATACAATTTCCAACTACGCAACAGATGGCAAGACAAGGACATGGTGAGAACAGCGTTTTCCATCAGGTCAACTGGTGTAGAGTGATACTTGATGAAGCTCCTGCCCTTAAGGATCCTTTGGACAAATTAGGTGCTCAGGCTGCTTATACCTTGTCCTCACATTGCCGATGGTGGTTAACAGGAACCCCAATACAGGGCACAGTGGAGGAACGCATGCAACAAGCTCAAGCCAGGTCGAAGCCAATGAATGCCCGTGTGAAAATTCGATCTGCCAGAATTGAGAAGTTCAAAATGCTTTTCACTTTGAATCTCATTGTTTTACATGTTACCGAAATTCAAATAGAAAGTCAAGACATCCCCAGACCTACCTCCGGCCCCCGCCGCCAGCCCAGCCGTCTCCAGCGCCGCGCTCCTCCCCCGGCCTCCCTCCAAATAAACCCCGTCGCCGACTGGAACGTCGCCATCCCCCTCCTCTCTCCTCTCGTCTCCCCCGATCGTCCGGCCGACTCCAAACCCCGTGACGACTCCCGCCACCACCACCACCAGAAACTCCAGAAACCCGACCTGCCACGTCATCAGGCCGCGGAGGCCGAGAAGTCGTCATCGTCATCGTCGTCGGCGGTATTTAAGAAGTGGCAGCACCCGGCGGCCCCCTTCTGCTACGAGCCTGCTCCGCCGGTCCGGCCTTTTGTTCCCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

407

Amino Acids

45.62

Weight (kDa)

8.82

Isoelectric Point (pI)

60.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 189 - 243 1.9e-08 SNF2-related domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010137)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04330 AT3G23170 AT4G14450
fragaria_vesca FvH4_4g03100 FvH4_7g33480
malus_domestica MD13G1210200.v1.1 MD16G1213100.v1.1
prunus_persica Prupe.1G034700_v2.0.a1
pyrus_communis pycom13g18280
rosa_chinensis RchiOBHm_Chr4g0391391
rosa_laevigata RLG00000009867
rosa_multiflora Rmu_sc0007466.1_g000006
rosa_roxburghii Rroxscaffold_5G00337120
rosa_rugosa Rorug03G0335800
rosa_samantha Rh4AG037400 Rh4BG031600 Rh4BG031700 Rh4CG040300 Rh4CG040400 Rh4DG034900
rosa_wichuraiana Rw4G002900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 974
Acc36I ACCTGC 1 cut(s) 1092
AccB1I GGYRCC 1 cut(s) 415
AccII CGCG 2 cut(s) 927, 1103
AclI AACGTT 1 cut(s) 127
AclWI GGATC 2 cut(s) 626, 639
AcoI YGGCCR 2 cut(s) 292, 1020
AcsI RAATTY 2 cut(s) 780, 852
AfaI GTAC 1 cut(s) 397
AfiI CCNNNNNNNGG 3 cut(s) 712, 713, 890
AflII CTTAAG 1 cut(s) 626
AflIII ACRYGT 1 cut(s) 841
AgsI TTSAA 6 cut(s) 125, 209, 492, 808, 826, 857
AjiI CACGTC 1 cut(s) 1091
AjnI CCWGG 2 cut(s) 511, 749
AluBI AGCT 2 cut(s) 617, 743
AluI AGCT 2 cut(s) 617, 743
Alw21I GWGCWC 1 cut(s) 655
Alw26I GTCTC 3 cut(s) 157, 920, 1009
AlwI GGATC 2 cut(s) 626, 639
AoxI GGCC 8 cut(s) 292, 892, 939, 1020, 1098, 1107, 1167, 1202
ApeKI GCWGC 2 cut(s) 659, 1156
ApoI RAATTY 2 cut(s) 780, 852
ArsI GACNNNNNNTTYG 2 cut(s) 850, 882
AspLEI GCGC 2 cut(s) 924, 929
AspS9I GGNCC 3 cut(s) 893, 1168, 1198
AsuC2I CCSGG 2 cut(s) 938, 1163
AsuHPI GGTGA 1 cut(s) 575
AvaII GGWCC 1 cut(s) 1198
BaeGI GKGCMC 1 cut(s) 719
BalI TGGCCA 1 cut(s) 294
BamHI GGATCC 1 cut(s) 631
BanI GGYRCC 1 cut(s) 415
BanII GRGCYC 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 655
BbvI GCAGC 2 cut(s) 646, 1168
BccI CCATC 6 cut(s) 236, 376, 538, 588, 681, 989
BceAI ACGGC 2 cut(s) 10, 897
BciT130I CCWGG 2 cut(s) 513, 751
BciVI GTATCC 1 cut(s) 199
BcnI CCSGG 2 cut(s) 938, 1163
BcoDI GTCTC 3 cut(s) 157, 920, 1009
BfaI CTAG 1 cut(s) 1219
BfmI CTRYAG 1 cut(s) 439
BfoI RGCGCY 1 cut(s) 925
BfrI CTTAAG 1 cut(s) 626
BfuAI ACCTGC 1 cut(s) 1092
BfuI GTATCC 1 cut(s) 199
BglI GCCNNNNNGGC 2 cut(s) 1106, 1201
BisI GCNGC 6 cut(s) 660, 901, 925, 1101, 1157, 1167
BlsI GCNGC 6 cut(s) 661, 902, 926, 1102, 1158, 1168
BmcAI AGTACT 1 cut(s) 397
Bme1390I CCNGG 4 cut(s) 513, 751, 938, 1163
Bme18I GGWCC 1 cut(s) 1198
BmgBI CACGTC 1 cut(s) 1091
BmgT120I GGNCC 3 cut(s) 893, 1168, 1198
BmiI GGNNCC 7 cut(s) 387, 417, 517, 633, 703, 895, 1170
BmrFI CCNGG 4 cut(s) 513, 751, 938, 1163
BmsI GCATC 3 cut(s) 84, 160, 266
BpmI CTGGAG 2 cut(s) 902, 1055
Bpu10I CCTNAGC 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 729
BpuMI CCSGG 2 cut(s) 938, 1163
BsaJI CCNNGG 2 cut(s) 936, 1101
Bsc4I CCNNNNNNNGG 3 cut(s) 712, 713, 890
Bse118I RCCGGY 1 cut(s) 1195
Bse1I ACTGG 3 cut(s) 171, 596, 974
Bse3DI GCAATG 2 cut(s) 61, 679
BseBI CCWGG 2 cut(s) 513, 751
BseDI CCNNGG 2 cut(s) 936, 1101
BseGI GGATG 4 cut(s) 175, 450, 873, 981
BseLI CCNNNNNNNGG 3 cut(s) 712, 713, 890
BseMI GCAATG 2 cut(s) 61, 679
BseMII CTCAG 2 cut(s) 246, 668
BseNI ACTGG 3 cut(s) 171, 596, 974
BseRI GAGGAG 4 cut(s) 392, 921, 980, 987
BseSI GKGCMC 1 cut(s) 719
BseX3I CGGCCG 1 cut(s) 1020
BseXI GCAGC 2 cut(s) 646, 1168
BseYI CCCAGC 1 cut(s) 908
Bsh1236I CGCG 2 cut(s) 927, 1103
Bsh1285I CGRYCG 2 cut(s) 1015, 1023
BshFI GGCC 8 cut(s) 294, 894, 941, 1022, 1100, 1109, 1169, 1204
BshNI GGYRCC 1 cut(s) 415
BsiEI CGRYCG 2 cut(s) 1015, 1023
BsiHKAI GWGCWC 1 cut(s) 655
BsiSI CCGG 6 cut(s) 891, 938, 1019, 1163, 1196, 1201
BslI CCNNNNNNNGG 3 cut(s) 712, 713, 890
BsmAI GTCTC 3 cut(s) 157, 920, 1009
BsmBI CGTCTC 2 cut(s) 920, 1009
BsmI GAATGC 1 cut(s) 772
BsnI GGCC 8 cut(s) 294, 894, 941, 1022, 1100, 1109, 1169, 1204
Bsp1286I GDGCHC 3 cut(s) 390, 655, 719
Bsp143I GATC 5 cut(s) 102, 409, 631, 785, 1012
BspANI GGCC 8 cut(s) 294, 894, 941, 1022, 1100, 1109, 1169, 1204
BspCNI CTCAG 2 cut(s) 245, 667
BspFNI CGCG 2 cut(s) 927, 1103
BspLI GGNNCC 7 cut(s) 387, 417, 517, 633, 703, 895, 1170
BspMI ACCTGC 1 cut(s) 1092
BspPI GGATC 2 cut(s) 626, 639
BspT107I GGYRCC 1 cut(s) 415
BspTI CTTAAG 1 cut(s) 626
BsrDI GCAATG 2 cut(s) 61, 679
BsrFI RCCGGY 1 cut(s) 1195
BsrI ACTGG 3 cut(s) 171, 596, 974
BssAI RCCGGY 1 cut(s) 1195
BssECI CCNNGG 2 cut(s) 936, 1101
BssMI GATC 5 cut(s) 102, 409, 631, 785, 1012
Bst2UI CCWGG 2 cut(s) 513, 751
Bst4CI ACNGT 1 cut(s) 721
Bst6I CTCTTC 1 cut(s) 231
BstAFI CTTAAG 1 cut(s) 626
BstC8I GCNNGC 4 cut(s) 292, 734, 905, 1188
BstDEI CTNAG 2 cut(s) 232, 654
BstDSI CCRYGG 1 cut(s) 1101
BstF5I GGATG 4 cut(s) 175, 450, 873, 981
BstFNI CGCG 2 cut(s) 927, 1103
BstH2I RGCGCY 1 cut(s) 925
BstHHI GCGC 2 cut(s) 924, 929
BstKTI GATC 5 cut(s) 105, 412, 634, 788, 1015
BstMAI GTCTC 3 cut(s) 157, 920, 1009
BstMBI GATC 5 cut(s) 102, 409, 631, 785, 1012
BstMCI CGRYCG 2 cut(s) 1015, 1023
BstMWI GCNNNNNNNGC 6 cut(s) 659, 900, 909, 1106, 1192, 1201
BstNI CCWGG 2 cut(s) 513, 751
BstNSI RCATGY 3 cut(s) 508, 736, 845
BstSCI CCNGG 4 cut(s) 511, 749, 936, 1161
BstSFI CTRYAG 1 cut(s) 439
BstSLI GKGCMC 1 cut(s) 719
BstUI CGCG 2 cut(s) 927, 1103
BstV1I GCAGC 2 cut(s) 646, 1168
BstX2I RGATCY 1 cut(s) 631
BstYI RGATCY 1 cut(s) 631
BstZI CGGCCG 1 cut(s) 1020
BsuI GTATCC 1 cut(s) 199
BsuRI GGCC 8 cut(s) 294, 894, 941, 1022, 1100, 1109, 1169, 1204
BtgI CCRYGG 1 cut(s) 1101
BtrI CACGTC 1 cut(s) 1091
BtsCI GGATG 4 cut(s) 175, 450, 873, 981
BtsIMutI CAGTG 1 cut(s) 726
BveI ACCTGC 1 cut(s) 1092
Cac8I GCNNGC 4 cut(s) 292, 734, 905, 1188
CfoI GCGC 2 cut(s) 924, 929
Cfr10I RCCGGY 1 cut(s) 1195
Cfr13I GGNCC 3 cut(s) 893, 1168, 1198
Cfr42I CCGCGG 1 cut(s) 1104
CpoI CGGWCCG 1 cut(s) 1198
Csp6I GTAC 1 cut(s) 396
CspI CGGWCCG 1 cut(s) 1198
CviAII CATG 4 cut(s) 505, 560, 733, 842
CviQI GTAC 1 cut(s) 396
DdeI CTNAG 2 cut(s) 232, 654
DpnI GATC 5 cut(s) 104, 411, 633, 787, 1014
DpnII GATC 5 cut(s) 102, 409, 631, 785, 1012
DraI TTTAAA 1 cut(s) 339
DrdI GACNNNNNNGTC 1 cut(s) 974
DseDI GACNNNNNNGTC 1 cut(s) 974
EaeI YGGCCR 2 cut(s) 292, 1020
EagI CGGCCG 1 cut(s) 1020
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
EciI GGCGGA 1 cut(s) 1182
EclXI CGGCCG 1 cut(s) 1020
Eco24I GRGCYC 1 cut(s) 390
Eco47I GGWCC 1 cut(s) 1198
Eco52I CGGCCG 1 cut(s) 1020
EcoRII CCWGG 2 cut(s) 511, 749
EcoT38I GRGCYC 1 cut(s) 390
Esp3I CGTCTC 2 cut(s) 920, 1009
FaeI CATG 4 cut(s) 508, 563, 736, 845
FatI CATG 4 cut(s) 504, 559, 732, 841
FauI CCCGC 3 cut(s) 454, 905, 1055
FauNDI CATATG 1 cut(s) 404
Fnu4HI GCNGC 6 cut(s) 660, 901, 925, 1101, 1157, 1167
FokI GGATG 4 cut(s) 182, 457, 860, 968
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 6 cut(s) 660, 901, 925, 1101, 1157, 1167
FspBI CTAG 1 cut(s) 1219
GlaI GCGC 2 cut(s) 923, 928
GluI GCNGC 6 cut(s) 660, 901, 925, 1101, 1157, 1167
GsaI CCCAGC 1 cut(s) 912
GsuI CTGGAG 2 cut(s) 902, 1055
HaeII RGCGCY 1 cut(s) 925
HaeIII GGCC 8 cut(s) 294, 894, 941, 1022, 1100, 1109, 1169, 1204
HapII CCGG 6 cut(s) 891, 938, 1019, 1163, 1196, 1201
HhaI GCGC 2 cut(s) 924, 929
Hin1II CATG 4 cut(s) 508, 563, 736, 845
Hin6I GCGC 2 cut(s) 922, 927
HinP1I GCGC 2 cut(s) 922, 927
HincII GTYRAC 3 cut(s) 475, 589, 696
HindII GTYRAC 3 cut(s) 475, 589, 696
HinfI GANTC 4 cut(s) 301, 826, 1025, 1043
HpaI GTTAAC 1 cut(s) 696
HpaII CCGG 6 cut(s) 891, 938, 1019, 1163, 1196, 1201
HphI GGTGA 1 cut(s) 575
Hpy166II GTNNAC 5 cut(s) 74, 136, 475, 589, 696
Hpy188I TCNGA 5 cut(s) 59, 235, 328, 358, 376
Hpy188III TCNNGA 5 cut(s) 148, 246, 305, 869, 1072
Hpy8I GTNNAC 5 cut(s) 74, 136, 475, 589, 696
Hpy99I CGWCG 4 cut(s) 965, 980, 1135, 1138
HpyAV CCTTC 1 cut(s) 1183
HpyCH4III ACNGT 1 cut(s) 721
HpyCH4IV ACGT 3 cut(s) 127, 975, 1090
HpyCH4V TGCA 5 cut(s) 66, 97, 257, 451, 736
HpyF10VI GCNNNNNNNGC 6 cut(s) 659, 900, 909, 1106, 1192, 1201
HpyF3I CTNAG 2 cut(s) 232, 654
HpySE526I ACGT 3 cut(s) 127, 975, 1090
Hsp92II CATG 4 cut(s) 508, 563, 736, 845
HspAI GCGC 2 cut(s) 922, 927
KspAI GTTAAC 1 cut(s) 696
KspI CCGCGG 1 cut(s) 1104
Kzo9I GATC 5 cut(s) 102, 409, 631, 785, 1012
LmnI GCTCC 4 cut(s) 385, 622, 934, 1195
Lsp1109I GCAGC 2 cut(s) 646, 1168
LweI GCATC 3 cut(s) 84, 160, 266
MaeI CTAG 1 cut(s) 1219
MaeII ACGT 3 cut(s) 127, 975, 1090
MaeIII GTNAC 2 cut(s) 844, 1037
MalI GATC 5 cut(s) 104, 411, 633, 787, 1014
MboI GATC 5 cut(s) 102, 409, 631, 785, 1012
MboII GAAGA 2 cut(s) 248, 320
MflI RGATCY 1 cut(s) 631
MhlI GDGCHC 3 cut(s) 390, 655, 719
MlsI TGGCCA 1 cut(s) 294
MluCI AATT 8 cut(s) 34, 210, 452, 524, 644, 780, 795, 852
MluNI TGGCCA 1 cut(s) 294
MlyI GAGTC 2 cut(s) 1019, 1037
MmeI TCCRAC 3 cut(s) 248, 343, 554
Mox20I TGGCCA 1 cut(s) 294
MscI TGGCCA 1 cut(s) 294
MseI TTAA 4 cut(s) 338, 627, 695, 1146
Msp20I TGGCCA 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 1103
MspCI CTTAAG 1 cut(s) 626
MspI CCGG 6 cut(s) 891, 938, 1019, 1163, 1196, 1201
MspR9I CCNGG 4 cut(s) 513, 751, 938, 1163
MteI GCGCNGCGC 1 cut(s) 925
Mva1269I GAATGC 1 cut(s) 772
MvaI CCWGG 2 cut(s) 513, 751
MvnI CGCG 2 cut(s) 927, 1103
MwoI GCNNNNNNNGC 6 cut(s) 659, 900, 909, 1106, 1192, 1201
NciI CCSGG 2 cut(s) 938, 1163
NdeI CATATG 1 cut(s) 404
NdeII GATC 5 cut(s) 102, 409, 631, 785, 1012
NlaIII CATG 4 cut(s) 508, 563, 736, 845
NlaIV GGNNCC 7 cut(s) 387, 417, 517, 633, 703, 895, 1170
NmeAIII GCCGAG 1 cut(s) 1135
NmuCI GTSAC 1 cut(s) 1037
NspI RCATGY 3 cut(s) 508, 736, 845
PaeI GCATGC 1 cut(s) 736
PciI ACATGT 1 cut(s) 841
PcsI WCGNNNNNNNCGW 2 cut(s) 1008, 1130
PctI GAATGC 1 cut(s) 772
PfeI GAWTC 2 cut(s) 301, 826
PfoI TCCNGGA 1 cut(s) 511
PkrI GCNGC 6 cut(s) 661, 902, 926, 1102, 1158, 1168
Ple19I CGATCG 1 cut(s) 1015
PleI GAGTC 2 cut(s) 1019, 1037
PpsI GAGTC 2 cut(s) 1019, 1037
PscI ACATGT 1 cut(s) 841
Psp1406I AACGTT 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 511, 749
PspFI CCCAGC 1 cut(s) 908
PspGI CCWGG 2 cut(s) 511, 749
PspN4I GGNNCC 7 cut(s) 387, 417, 517, 633, 703, 895, 1170
PspPI GGNCC 3 cut(s) 893, 1168, 1198
PsuI RGATCY 1 cut(s) 631
PvuI CGATCG 1 cut(s) 1015
RsaI GTAC 1 cut(s) 397
RsaNI GTAC 1 cut(s) 396
Rsr2I CGGWCCG 1 cut(s) 1198
RsrII CGGWCCG 1 cut(s) 1198
SacII CCGCGG 1 cut(s) 1104
SaqAI TTAA 4 cut(s) 338, 627, 695, 1146
SatI GCNGC 6 cut(s) 660, 901, 925, 1101, 1157, 1167
Sau3AI GATC 5 cut(s) 102, 409, 631, 785, 1012
Sau96I GGNCC 3 cut(s) 893, 1168, 1198
ScaI AGTACT 1 cut(s) 397
SchI GAGTC 2 cut(s) 1019, 1037
ScrFI CCNGG 4 cut(s) 513, 751, 938, 1163
SduI GDGCHC 3 cut(s) 390, 655, 719
SfaNI GCATC 3 cut(s) 84, 160, 266
SfcI CTRYAG 1 cut(s) 439
SfiI GGCCNNNNNGGCC 1 cut(s) 1106
Sfr303I CCGCGG 1 cut(s) 1104
SgrBI CCGCGG 1 cut(s) 1104
SinI GGWCC 1 cut(s) 1198
SmlI CTYRAG 2 cut(s) 626, 744
SmoI CTYRAG 2 cut(s) 626, 744
SphI GCATGC 1 cut(s) 736
Sse9I AATT 8 cut(s) 34, 210, 452, 524, 644, 780, 795, 852
SspMI CTAG 1 cut(s) 1219
StyD4I CCNGG 4 cut(s) 511, 749, 936, 1161
TaaI ACNGT 1 cut(s) 721
TaiI ACGT 3 cut(s) 130, 978, 1093
TaqI TCGA 2 cut(s) 755, 784
TasI AATT 8 cut(s) 34, 210, 452, 524, 644, 780, 795, 852
TatI WGTACW 1 cut(s) 395
TauI GCSGC 4 cut(s) 903, 927, 1103, 1169
TfiI GAWTC 2 cut(s) 301, 826
Tru1I TTAA 4 cut(s) 338, 627, 695, 1146
Tru9I TTAA 4 cut(s) 338, 627, 695, 1146
TscAI CASTG 1 cut(s) 726
TseFI GTSAC 1 cut(s) 1037
TseI GCWGC 2 cut(s) 659, 1156
Tsp45I GTSAC 1 cut(s) 1037
TspDTI ATGAA 3 cut(s) 185, 627, 779
TspGWI ACGGA 1 cut(s) 121
TspRI CASTG 1 cut(s) 726
Vha464I CTTAAG 1 cut(s) 626
VpaK11BI GGWCC 1 cut(s) 1198
XapI RAATTY 2 cut(s) 780, 852
XceI RCATGY 3 cut(s) 508, 736, 845
XspI CTAG 1 cut(s) 1219
ZrmI AGTACT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.