AT4G15530

Belongs to the PEP-utilizing enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004385
Physical Location & Seq
Reverse (-)
8864615 .. 8870979
6365 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G15530.3

Sequence Viewer

Length: 2871 bp
ATGACAAGTATGATCGTGAAGACAACGCCGGAGCTCTTCAAAGGAAATGGAGTGTTCCGTACGGATCATCTCGGAGAAAACCGAATGGTTAGTCGATCAAACCGGCTAGGTGATGGATCAAACCGTTTCCCTAGAACCGGTACAATCCATTGCCAACGGTTAAGCATAGCAAAGACCGGTTTGCATCGTGAGACGAAGGCTCGAGCCATACTTAGCCCTGTGTCCGATCCGGCCGCTTCCATAGCCCAAAAGCGAGTATTCACCTTTGGAAAAGGAAGAAGCGAAGGCAACAAGGGCATGAAGTCCTTGTTGGGAGGGAAAGGAGCCAACCTGGCGGAGATGGCTAGCATAGGCTTGTCGGTGCCGCCGGGGCTAACCATATCGACGGAGGCTTGTCAGCAGTATCAGATCGCCGGCAAAAAGCTTCCAGAAGGTTTATGGGAAGAGATCTTAGAAGGTCTTAGCTTCATCGAACGTGACATTGGAGCTTCCCTCGCTGATCCCTCCAAGCCACTCCTCCTCTCTGTTCGCTCCGGCGCCGCCATCTCAATGCCTGGTATGATGGACACTGTACTTAACCTTGGCTTGAACGACCAAGTCGTCGTTGGTCTGGCCGCAAAAAGCGGAGAGCGTTTTGCTTACGATTCGTTCCGGCGTTTTCTTGATATGTTTGGTGATGTTGTGATGGGAATTCCACACGCCAAGTTTGAAGAGAAGTTAGAGAGAATGAAGGAGAGGAAAGGAGTTAAAAATGACACTGACTTAAGCGCGGCTGATCTCAAGGAATTGGTTGAGCAGTACAAGAGTGTTTACTTAGAGGCCAAGGGTCAAGAGTTTCCTTCAGATCCAAAGAAGCAATTGGAGCTAGCGATTGAAGCGGTATTCGATTCTTGGGATAGCCCGAGAGCGAACAAGTACAGAAGTATTAACCAGATAACTGGATTGAAAGGAACCGCGGTTAACATTCAGTGTATGGTGTTTGGAAACATGGGGGACACTTCAGGGACTGGTGTTCTCTTCACTAGGAACCCTAGCACAGGAGAGAAGAAGCTTTATGGCGAGTTTCTAGTTAATGCTCAGGGAGAGGATGTGGTTGCAGGGATAAGAACACCAGAAGATTTGGATACAATGAAGAGATTTATGCCTGAGGCTTACGCTGAACTTGTTGAGAACTGCAACATCTTAGAAAGACATTACAAAGACATGATGGATATTGAATTCACAGTACAAGAAGAGAGATTGTGGATGCTGCAATGCAGAGCGGGTAAGCGAACGGGTAAAGGCGCCGTGAAGATAGCAGTTGATATGGTAGGTGAAGGGCTTGTTGAGAAATCTTCTGCTATCAAAATGGTGGAGCCTCAACATCTTGATCAACTACTTCACCCACAGTTTCATGATCCATCGGGGTATCGTGAAAAAGTGGTGGCCAAAGGCTTACCTGCGTCACCAGGAGCGGCGGTTGGACAGGTTGTGTTCACGGCGGAGGAAGCCGAAGCTTGGCATTCTCAGGGTAAAACTGTGATTCTGGTTCGAACTGAGACAAGCCCTGACGATGTGGGAGGTATGCACGCAGCGGAAGGTATATTGACGGCTAGAGGAGGAATGACGTCACACGCGGCTGTTGTTGCTCGCGGTTGGGGAAAATGTTGCATTGCTGGTTGTTCCGAGATTCGTGTCGACGAGAACCACAAGGTTCTATTGATTGGAGATTTGACGATTAATGAAGGCGAATGGATCTCAATGAACGGATCAACCGGTGAGGTTATATTAGGGAAACAAGCATTGGCTCCTCCGGCTTTAAGTCCAGATTTGGAGACTTTCATGTCCTGGGCTGATGCAATCAGACGTCTCAAGGTTATGGCGAATGCGGATACACCTGAAGACGCCATTGCAGCTAGGAAAAACGGAGCTCAAGGAATCGGGCTTTGTAGGACAGAGCATATGTTCTTTGGAGCAGATAGGATTAAAGCAGTGAGAAAGATGATAATGGCGGTAACAACAGAGCAAAGGAAAGCTTCTCTCGACATCTTGCTTCCTTACCAACGTTCGGATTTCGAAGGGATCTTCCGTGCTATGGATGGTTTACCGGTAACAATCCGTTTGTTAGACCCTCCGCTTCACGAGTTTCTCCCGGAAGGCGACTTGGACAACATTGTACATGAGCTAGCTGAAGAAACTGGTGTGAAAGAAGATGAAGTCTTGTCACGGATAGAGAAACTCTCTGAAGTGAATCCAATGCTTGGTTTCCGCGGTTGCAGGCTCGGAATATCGTATCCAGAGCTAACGGAGATGCAAGCGCGTGCAATTTTTGAAGCTGCAGCGTCAATGCAGGACCAAGGTGTTACTGTCATTCCTGAGATTATGGTTCCACTTGTAGGAACTCCTCAGGAATTGGGTCACCAAGTTGATGTAATTCGTAAAGTTGCAAAGAAAGTATTTGCTGAGAAGGGTCATACCGTGAGCTACAAGGTTGGGACAATGATTGAGATCCCTCGAGCCGCGCTCATTGCAGATGAGATTGCGAAAGAGGCGGAGTTTTTCTCGTTCGGGACAAACGACTTGACGCAGATGACGTTTGGATACAGTAGAGACGATGTCGGCAAGTTTCTACCGATTTACCTCGCCAAAGGAATCTTACAGCACGACCCTTTTGAGGTTCTTGATCAGCAAGGTGTAGGGCAATTGATCAAGATGGCGACAGAAAAAGGACGAGCAGCTAGGCCTAGCCTCAAGGTTGGGATATGTGGAGAACATGGAGGAGATCCATCTTCTGTGGGATTCTTTGCTGAAGCAGGACTTGACTATGTCTCTTGTTCTCCTTTCAGGGTTCCAATTGCAAGGCTTGCAGCTGCTCAAGTAGTTGTTGCATGA

Protein Analysis

956

Amino Acids

104.2

Weight (kDa)

5.82

Isoelectric Point (pI)

34.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPDK_N PF01326 3 - 36 2.5e-06 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N PF01326 38 - 275 2.6e-22 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N PF01326 286 - 337 9e-10 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PEP-utilizers PF00391 407 - 487 5.5e-23 PEP-utilising enzyme, mobile domain
PEP-utilizers_C PF02896 504 - 854 6.4e-93 PEP-utilising enzyme, PEP-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 599, 1822
AatII GACGTC 2 cut(s) 1610, 1849
Acc36I ACCTGC 1 cut(s) 1447
AccB1I GGYRCC 3 cut(s) 361, 536, 1283
AccB7I CCANNNNNTGG 1 cut(s) 2240
AccBSI CCGCTC 2 cut(s) 1262, 1454
AccI GTMKAC 1 cut(s) 1677
AccII CGCG 7 cut(s) 770, 956, 1616, 1632, 2250, 2299, 2501
AclI AACGTT 1 cut(s) 2044
AcoI YGGCCR 3 cut(s) 231, 612, 1425
AcsI RAATTY 2 cut(s) 690, 1217
AcuI CTGAAG 6 cut(s) 825, 984, 1899, 2190, 2244, 2808
AcyI GRCGYC 5 cut(s) 537, 1284, 1607, 1846, 1884
AfaI GTAC 7 cut(s) 61, 142, 573, 800, 917, 1227, 2157
AfiI CCNNNNNNNGG 8 cut(s) 137, 1037, 1497, 2047, 2074, 2240, 2375, 2653
AflII CTTAAG 1 cut(s) 763
AgeI ACCGGT 4 cut(s) 137, 176, 1754, 2086
AgsI TTSAA 7 cut(s) 40, 589, 710, 875, 946, 1217, 2312
AjnI CCWGG 4 cut(s) 330, 553, 1447, 1826
AjuI GAANNNNNNNTTGG 6 cut(s) 293, 325, 465, 497, 1766, 1798
Alw21I GWGCWC 2 cut(s) 36, 1912
Alw26I GTCTC 6 cut(s) 185, 1532, 1808, 1853, 2583, 2812
AlwNI CAGNNNCTG 1 cut(s) 1007
Ama87I CYCGRG 3 cut(s) 201, 901, 2493
AoxI GGCC 5 cut(s) 231, 612, 819, 1425, 2720
ApeKI GCWGC 8 cut(s) 1249, 1571, 1892, 2315, 2318, 2714, 2846, 2849
ApoI RAATTY 2 cut(s) 690, 1217
ArsI GACNNNNNNTTYG 2 cut(s) 76, 108
AseI ATTAAT 1 cut(s) 1719
AsiGI ACCGGT 4 cut(s) 137, 176, 1754, 2086
AspLEI GCGC 5 cut(s) 539, 770, 1286, 2299, 2503
AspS9I GGNCC 1 cut(s) 2332
AsuC2I CCSGG 2 cut(s) 369, 2132
AsuHPI GGTGA 8 cut(s) 122, 253, 686, 1325, 1373, 1437, 1769, 2390
AsuII TTCGAA 2 cut(s) 1531, 2055
AsuNHI GCTAGC 3 cut(s) 344, 865, 2164
AvaI CYCGRG 3 cut(s) 201, 901, 2493
AvaII GGWCC 1 cut(s) 2332
AxyI CCTNAGG 2 cut(s) 1146, 2385
BalI TGGCCA 1 cut(s) 1427
BanI GGYRCC 3 cut(s) 361, 536, 1283
BanII GRGCYC 2 cut(s) 36, 1912
BauI CACGAG 1 cut(s) 2120
BbsI GAAGAC 2 cut(s) 26, 1887
Bbv12I GWGCWC 2 cut(s) 36, 1912
BbvI GCAGC 8 cut(s) 1236, 1583, 1904, 2302, 2330, 2726, 2836, 2858
BceAI ACGGC 3 cut(s) 1271, 1494, 1605
BciT130I CCWGG 4 cut(s) 332, 555, 1449, 1828
BciVI GTATCC 4 cut(s) 1117, 1864, 2283, 2573
BclI TGATCA 3 cut(s) 1369, 2662, 2685
BcnI CCSGG 2 cut(s) 369, 2132
BcoDI GTCTC 6 cut(s) 185, 1532, 1808, 1853, 2583, 2812
BfmI CTRYAG 1 cut(s) 2316
BfoI RGCGCY 2 cut(s) 540, 1287
BfrI CTTAAG 1 cut(s) 763
BfuAI ACCTGC 1 cut(s) 1447
BfuI GTATCC 4 cut(s) 1117, 1864, 2283, 2573
BglI GCCNNNNNGGC 2 cut(s) 332, 370
BglII AGATCT 1 cut(s) 447
Bme1390I CCNGG 6 cut(s) 332, 369, 555, 1449, 1828, 2132
Bme18I GGWCC 1 cut(s) 2332
BmeT110I CYCGRG 3 cut(s) 201, 901, 2493
BmgT120I GGNCC 1 cut(s) 2332
BmrFI CCNGG 6 cut(s) 332, 369, 555, 1449, 1828, 2132
BmsI GCATC 4 cut(s) 193, 1236, 1825, 2280
BmtI GCTAGC 3 cut(s) 348, 869, 2168
BpiI GAAGAC 2 cut(s) 26, 1887
BplI GAGNNNNNCTC 8 cut(s) 477, 509, 2204, 2236, 2487, 2519, 2525, 2557
Bpu10I CCTNAGC 1 cut(s) 1077
Bpu14I TTCGAA 2 cut(s) 1531, 2055
BpuEI CTTGAG 5 cut(s) 764, 1835, 1896, 2714, 2838
BpuMI CCSGG 2 cut(s) 369, 2132
BsaBI GATNNNNATC 1 cut(s) 2486
BsaHI GRCGYC 5 cut(s) 537, 1284, 1607, 1846, 1884
BsaJI CCNNGG 7 cut(s) 368, 580, 822, 954, 1827, 2248, 2335
BsaWI WCCGGW 4 cut(s) 137, 176, 1754, 2086
BsaXI ACNNNNNCTCC 4 cut(s) 1074, 1104, 1443, 1473
Bsc4I CCNNNNNNNGG 8 cut(s) 137, 1037, 1497, 2047, 2074, 2240, 2375, 2653
Bse118I RCCGGY 6 cut(s) 102, 137, 176, 413, 1754, 2086
Bse1I ACTGG 3 cut(s) 943, 1012, 2182
Bse21I CCTNAGG 2 cut(s) 1146, 2385
Bse3DI GCAATG 5 cut(s) 148, 1259, 1650, 1887, 2505
Bse8I GATNNNNATC 1 cut(s) 2486
BseBI CCWGG 4 cut(s) 332, 555, 1449, 1828
BseDI CCNNGG 7 cut(s) 368, 580, 822, 954, 1827, 2248, 2335
BseGI GGATG 3 cut(s) 1093, 1251, 2083
BseJI GATNNNNATC 1 cut(s) 2486
BseLI CCNNNNNNNGG 8 cut(s) 137, 1037, 1497, 2047, 2074, 2240, 2375, 2653
BseMI GCAATG 5 cut(s) 148, 1259, 1650, 1887, 2505
BseMII CTCAG 7 cut(s) 1091, 1137, 1520, 1527, 2346, 2399, 2433
BseNI ACTGG 3 cut(s) 943, 1012, 2182
BseRI GAGGAG 6 cut(s) 506, 509, 1611, 1779, 2373, 2772
BseX3I CGGCCG 1 cut(s) 231
BseXI GCAGC 8 cut(s) 1236, 1583, 1904, 2302, 2330, 2726, 2836, 2858
Bsh1236I CGCG 7 cut(s) 770, 956, 1616, 1632, 2250, 2299, 2501
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 5 cut(s) 233, 614, 821, 1427, 2722
BshNI GGYRCC 3 cut(s) 361, 536, 1283
BshTI ACCGGT 4 cut(s) 137, 176, 1754, 2086
BsiEI CGRYCG 1 cut(s) 234
BsiHKAI GWGCWC 2 cut(s) 36, 1912
BsiHKCI CYCGRG 3 cut(s) 201, 901, 2493
BsiWI CGTACG 1 cut(s) 59
BslFI GGGAC 4 cut(s) 1007, 1018, 2488, 2563
BslI CCNNNNNNNGG 8 cut(s) 137, 1037, 1497, 2047, 2074, 2240, 2375, 2653
BsmAI GTCTC 6 cut(s) 185, 1532, 1808, 1853, 2583, 2812
BsmBI CGTCTC 3 cut(s) 185, 1853, 2583
BsmFI GGGAC 4 cut(s) 1007, 1018, 2488, 2563
BsmI GAATGC 2 cut(s) 1501, 1870
BsnI GGCC 5 cut(s) 233, 614, 821, 1427, 2722
BsoBI CYCGRG 3 cut(s) 201, 901, 2493
Bsp119I TTCGAA 2 cut(s) 1531, 2055
Bsp1286I GDGCHC 2 cut(s) 36, 1912
Bsp1407I TGTACA 1 cut(s) 2155
BspANI GGCC 5 cut(s) 233, 614, 821, 1427, 2722
BspCNI CTCAG 7 cut(s) 1090, 1138, 1519, 1528, 2347, 2398, 2434
BspFNI CGCG 7 cut(s) 770, 956, 1616, 1632, 2250, 2299, 2501
BspHI TCATGA 1 cut(s) 1393
BspMAI CTGCAG 1 cut(s) 2320
BspMI ACCTGC 1 cut(s) 1447
BspOI GCTAGC 3 cut(s) 348, 869, 2168
BspQI GCTCTTC 1 cut(s) 41
BspT104I TTCGAA 2 cut(s) 1531, 2055
BspT107I GGYRCC 3 cut(s) 361, 536, 1283
BspTI CTTAAG 1 cut(s) 763
BsrBI CCGCTC 2 cut(s) 1262, 1454
BsrDI GCAATG 5 cut(s) 148, 1259, 1650, 1887, 2505
BsrFI RCCGGY 6 cut(s) 102, 137, 176, 413, 1754, 2086
BsrGI TGTACA 1 cut(s) 2155
BsrI ACTGG 3 cut(s) 943, 1012, 2182
BssAI RCCGGY 6 cut(s) 102, 137, 176, 413, 1754, 2086
BssECI CCNNGG 7 cut(s) 368, 580, 822, 954, 1827, 2248, 2335
BssNI GRCGYC 5 cut(s) 537, 1284, 1607, 1846, 1884
BssSI CACGAG 1 cut(s) 2120
BssT1I CCWWGG 3 cut(s) 580, 822, 2335
Bst2BI CACGAG 1 cut(s) 2120
Bst2UI CCWGG 4 cut(s) 332, 555, 1449, 1828
Bst4CI ACNGT 9 cut(s) 125, 159, 571, 1225, 1389, 1519, 2347, 2458, 2585
Bst6I CTCTTC 6 cut(s) 41, 438, 705, 1022, 1127, 1227
BstACI GRCGYC 5 cut(s) 537, 1284, 1607, 1846, 1884
BstAFI CTTAAG 1 cut(s) 763
BstAPI GCANNNNNTGC 1 cut(s) 2843
BstAUI TGTACA 1 cut(s) 2155
BstBI TTCGAA 2 cut(s) 1531, 2055
BstDSI CCRYGG 2 cut(s) 954, 2248
BstEII GGTNACC 1 cut(s) 2396
BstENI CCTNNNNNAGG 1 cut(s) 1035
BstF5I GGATG 3 cut(s) 1093, 1251, 2083
BstFNI CGCG 7 cut(s) 770, 956, 1616, 1632, 2250, 2299, 2501
BstH2I RGCGCY 2 cut(s) 540, 1287
BstHHI GCGC 5 cut(s) 539, 770, 1286, 2299, 2503
BstMAI GTCTC 6 cut(s) 185, 1532, 1808, 1853, 2583, 2812
BstMCI CGRYCG 1 cut(s) 234
BstNI CCWGG 4 cut(s) 332, 555, 1449, 1828
BstPI GGTNACC 1 cut(s) 2396
BstSCI CCNGG 6 cut(s) 330, 367, 553, 1447, 1826, 2130
BstSFI CTRYAG 1 cut(s) 2316
BstUI CGCG 7 cut(s) 770, 956, 1616, 1632, 2250, 2299, 2501
BstV1I GCAGC 8 cut(s) 1236, 1583, 1904, 2302, 2330, 2726, 2836, 2858
BstV2I GAAGAC 2 cut(s) 26, 1887
BstX2I RGATCY 6 cut(s) 447, 844, 1734, 2061, 2487, 2761
BstXI CCANNNNNNTGG 1 cut(s) 938
BstYI RGATCY 6 cut(s) 447, 844, 1734, 2061, 2487, 2761
BstZI CGGCCG 1 cut(s) 231
Bsu36I CCTNAGG 2 cut(s) 1146, 2385
BsuI GTATCC 4 cut(s) 1117, 1864, 2283, 2573
BsuRI GGCC 5 cut(s) 233, 614, 821, 1427, 2722
BtgI CCRYGG 2 cut(s) 954, 2248
BtsCI GGATG 3 cut(s) 1093, 1251, 2083
BtsI GCAGTG 1 cut(s) 1977
BtsIMutI CAGTG 4 cut(s) 567, 756, 974, 1977
BveI ACCTGC 1 cut(s) 1447
CaiI CAGNNNCTG 1 cut(s) 1007
CciI TCATGA 1 cut(s) 1393
CfoI GCGC 5 cut(s) 539, 770, 1286, 2299, 2503
Cfr10I RCCGGY 6 cut(s) 102, 137, 176, 413, 1754, 2086
Cfr13I GGNCC 1 cut(s) 2332
Cfr42I CCGCGG 2 cut(s) 957, 2251
CseI GACGC 4 cut(s) 1431, 1892, 2310, 2572
Csp6I GTAC 7 cut(s) 60, 141, 572, 799, 916, 1226, 2156
CspAI ACCGGT 4 cut(s) 137, 176, 1754, 2086
CviAII CATG 8 cut(s) 298, 988, 1204, 1394, 1822, 2159, 2753, 2868
CviQI GTAC 7 cut(s) 60, 141, 572, 799, 916, 1226, 2156
DinI GGCGCC 2 cut(s) 538, 1285
DrdI GACNNNNNNGTC 2 cut(s) 599, 1822
DseDI GACNNNNNNGTC 2 cut(s) 599, 1822
EaeI YGGCCR 3 cut(s) 231, 612, 1425
EagI CGGCCG 1 cut(s) 231
Eam1104I CTCTTC 6 cut(s) 41, 438, 705, 1022, 1127, 1227
EarI CTCTTC 6 cut(s) 41, 438, 705, 1022, 1127, 1227
EciI GGCGGA 3 cut(s) 350, 1496, 2546
Ecl136II GAGCTC 2 cut(s) 34, 1910
EclXI CGGCCG 1 cut(s) 231
Eco130I CCWWGG 3 cut(s) 580, 822, 2335
Eco147I AGGCCT 1 cut(s) 2722
Eco24I GRGCYC 2 cut(s) 36, 1912
Eco47I GGWCC 1 cut(s) 2332
Eco52I CGGCCG 1 cut(s) 231
Eco53kI GAGCTC 2 cut(s) 34, 1910
Eco57I CTGAAG 6 cut(s) 825, 984, 1899, 2190, 2244, 2808
Eco81I CCTNAGG 2 cut(s) 1146, 2385
Eco88I CYCGRG 3 cut(s) 201, 901, 2493
Eco91I GGTNACC 1 cut(s) 2396
EcoICRI GAGCTC 2 cut(s) 34, 1910
EcoNI CCTNNNNNAGG 1 cut(s) 1035
EcoO65I GGTNACC 1 cut(s) 2396
EcoRI GAATTC 2 cut(s) 690, 1217
EcoRII CCWGG 4 cut(s) 330, 553, 1447, 1826
EcoT14I CCWWGG 3 cut(s) 580, 822, 2335
EcoT38I GRGCYC 2 cut(s) 36, 1912
EgeI GGCGCC 2 cut(s) 538, 1285
EheI GGCGCC 2 cut(s) 538, 1285
ErhI CCWWGG 3 cut(s) 580, 822, 2335
Esp3I CGTCTC 3 cut(s) 185, 1853, 2583
FaeI CATG 8 cut(s) 301, 991, 1207, 1397, 1825, 2162, 2756, 2871
FalI AAGNNNNNCTT 4 cut(s) 1999, 2031, 2781, 2813
FaqI GGGAC 4 cut(s) 1007, 1018, 2488, 2563
FatI CATG 8 cut(s) 297, 987, 1203, 1393, 1821, 2158, 2752, 2867
FauI CCCGC 1 cut(s) 1255
FauNDI CATATG 1 cut(s) 1941
FbaI TGATCA 3 cut(s) 1369, 2662, 2685
FblI GTMKAC 1 cut(s) 1677
FokI GGATG 3 cut(s) 1100, 1258, 2090
FriOI GRGCYC 2 cut(s) 36, 1912
GlaI GCGC 5 cut(s) 538, 769, 1285, 2298, 2502
HaeII RGCGCY 2 cut(s) 540, 1287
HaeIII GGCC 5 cut(s) 233, 614, 821, 1427, 2722
HgaI GACGC 4 cut(s) 1431, 1892, 2310, 2572
HhaI GCGC 5 cut(s) 539, 770, 1286, 2299, 2503
Hin1I GRCGYC 5 cut(s) 537, 1284, 1607, 1846, 1884
Hin1II CATG 8 cut(s) 301, 991, 1207, 1397, 1825, 2162, 2756, 2871
Hin6I GCGC 5 cut(s) 537, 768, 1284, 2297, 2501
HinP1I GCGC 5 cut(s) 537, 768, 1284, 2297, 2501
HincII GTYRAC 2 cut(s) 961, 1678
HindII GTYRAC 2 cut(s) 961, 1678
HindIII AAGCTT 4 cut(s) 422, 1049, 1494, 2013
HinfI GANTC 8 cut(s) 644, 887, 1522, 1669, 1917, 2230, 2631, 2778
HpaI GTTAAC 1 cut(s) 961
HphI GGTGA 8 cut(s) 122, 253, 686, 1325, 1373, 1437, 1769, 2390
Hpy166II GTNNAC 5 cut(s) 811, 961, 1476, 1678, 2084
Hpy188I TCNGA 9 cut(s) 74, 226, 408, 844, 1666, 1844, 2050, 2224, 2264
Hpy8I GTNNAC 5 cut(s) 811, 961, 1476, 1678, 2084
Hpy99I CGWCG 3 cut(s) 388, 605, 1682
HpyCH4III ACNGT 9 cut(s) 125, 159, 571, 1225, 1389, 1519, 2347, 2458, 2585
HpyCH4IV ACGT 5 cut(s) 475, 1607, 1846, 2044, 2573
HpySE526I ACGT 5 cut(s) 475, 1607, 1846, 2044, 2573
Hsp92I GRCGYC 5 cut(s) 537, 1284, 1607, 1846, 1884
Hsp92II CATG 8 cut(s) 301, 991, 1207, 1397, 1825, 2162, 2756, 2871
HspAI GCGC 5 cut(s) 537, 768, 1284, 2297, 2501
KasI GGCGCC 2 cut(s) 536, 1283
KroI GCCGGC 1 cut(s) 413
KroNI GCCGGC 1 cut(s) 415
Ksp22I TGATCA 3 cut(s) 1369, 2662, 2685
KspAI GTTAAC 1 cut(s) 961
KspI CCGCGG 2 cut(s) 957, 2251
LguI GCTCTTC 1 cut(s) 41
Lsp1109I GCAGC 8 cut(s) 1236, 1583, 1904, 2302, 2330, 2726, 2836, 2858
LweI GCATC 4 cut(s) 193, 1236, 1825, 2280
MaeII ACGT 5 cut(s) 475, 1607, 1846, 2044, 2573
MaeIII GTNAC 8 cut(s) 476, 1443, 1608, 1993, 2089, 2202, 2341, 2396
MbiI CCGCTC 2 cut(s) 1262, 1454
MfeI CAATTG 3 cut(s) 857, 2681, 2832
MflI RGATCY 6 cut(s) 447, 844, 1734, 2061, 2487, 2761
MhlI GDGCHC 2 cut(s) 36, 1912
MlsI TGGCCA 1 cut(s) 1427
MluCI AATT 9 cut(s) 690, 785, 857, 1217, 2304, 2390, 2412, 2681, 2832
MluNI TGGCCA 1 cut(s) 1427
Mly113I GGCGCC 2 cut(s) 537, 1284
MmeI TCCRAC 1 cut(s) 1441
Mox20I TGGCCA 1 cut(s) 1427
MroNI GCCGGC 1 cut(s) 413
MscI TGGCCA 1 cut(s) 1427
MslI CAYNNNNRTG 1 cut(s) 548
Msp20I TGGCCA 1 cut(s) 1427
MspA1I CMGCKG 4 cut(s) 956, 1574, 2250, 2849
MspCI CTTAAG 1 cut(s) 763
MspR9I CCNGG 6 cut(s) 332, 369, 555, 1449, 1828, 2132
MunI CAATTG 3 cut(s) 857, 2681, 2832
Mva1269I GAATGC 2 cut(s) 1501, 1870
MvaI CCWGG 4 cut(s) 332, 555, 1449, 1828
MvnI CGCG 7 cut(s) 770, 956, 1616, 1632, 2250, 2299, 2501
NaeI GCCGGC 1 cut(s) 415
NarI GGCGCC 2 cut(s) 537, 1284
NciI CCSGG 2 cut(s) 369, 2132
NdeI CATATG 1 cut(s) 1941
NgoMIV GCCGGC 1 cut(s) 413
NheI GCTAGC 3 cut(s) 344, 865, 2164
NlaIII CATG 8 cut(s) 301, 991, 1207, 1397, 1825, 2162, 2756, 2871
NmuCI GTSAC 5 cut(s) 476, 1443, 1608, 2202, 2396
NspV TTCGAA 2 cut(s) 1531, 2055
PaeR7I CTCGAG 2 cut(s) 201, 2493
PagI TCATGA 1 cut(s) 1393
PceI AGGCCT 1 cut(s) 2722
PciSI GCTCTTC 1 cut(s) 41
PcsI WCGNNNNNNNCGW 3 cut(s) 597, 2553, 2570
PctI GAATGC 2 cut(s) 1501, 1870
PdiI GCCGGC 1 cut(s) 415
PfeI GAWTC 8 cut(s) 644, 887, 1522, 1669, 1917, 2230, 2631, 2778
Pfl23II CGTACG 1 cut(s) 59
PflFI GACNNNGTC 3 cut(s) 596, 2594, 2804
PflMI CCANNNNNTGG 1 cut(s) 2240
PfoI TCCNGGA 1 cut(s) 2130
PinAI ACCGGT 4 cut(s) 137, 176, 1754, 2086
PluTI GGCGCC 2 cut(s) 540, 1287
PshBI ATTAAT 1 cut(s) 1719
Psp124BI GAGCTC 2 cut(s) 36, 1912
Psp1406I AACGTT 1 cut(s) 2044
Psp6I CCWGG 4 cut(s) 330, 553, 1447, 1826
PspEI GGTNACC 1 cut(s) 2396
PspGI CCWGG 4 cut(s) 330, 553, 1447, 1826
PspLI CGTACG 1 cut(s) 59
PspPI GGNCC 1 cut(s) 2332
PspXI VCTCGAGB 2 cut(s) 201, 2493
PstI CTGCAG 1 cut(s) 2320
PstNI CAGNNNCTG 1 cut(s) 1007
PsuI RGATCY 6 cut(s) 447, 844, 1734, 2061, 2487, 2761
PsyI GACNNNGTC 3 cut(s) 596, 2594, 2804
PvuII CAGCTG 1 cut(s) 2849
RsaI GTAC 7 cut(s) 61, 142, 573, 800, 917, 1227, 2157
RsaNI GTAC 7 cut(s) 60, 141, 572, 799, 916, 1226, 2156
RseI CAYNNNNRTG 1 cut(s) 548
SacI GAGCTC 2 cut(s) 36, 1912
SacII CCGCGG 2 cut(s) 957, 2251
SalI GTCGAC 1 cut(s) 1676
SapI GCTCTTC 1 cut(s) 41
Sau96I GGNCC 1 cut(s) 2332
ScrFI CCNGG 6 cut(s) 332, 369, 555, 1449, 1828, 2132
SduI GDGCHC 2 cut(s) 36, 1912
SfaNI GCATC 4 cut(s) 193, 1236, 1825, 2280
SfcI CTRYAG 1 cut(s) 2316
SfoI GGCGCC 2 cut(s) 538, 1285
Sfr274I CTCGAG 2 cut(s) 201, 2493
Sfr303I CCGCGG 2 cut(s) 957, 2251
SfuI TTCGAA 2 cut(s) 1531, 2055
SgrBI CCGCGG 2 cut(s) 957, 2251
SinI GGWCC 1 cut(s) 2332
SlaI CTCGAG 2 cut(s) 201, 2493
SmiMI CAYNNNNRTG 1 cut(s) 548
SmlI CTYRAG 8 cut(s) 201, 763, 779, 1850, 1911, 2493, 2729, 2853
SmoI CTYRAG 8 cut(s) 201, 763, 779, 1850, 1911, 2493, 2729, 2853
Sse9I AATT 9 cut(s) 690, 785, 857, 1217, 2304, 2390, 2412, 2681, 2832
SseBI AGGCCT 1 cut(s) 2722
SspDI GGCGCC 2 cut(s) 536, 1283
SstI GAGCTC 2 cut(s) 36, 1912
StuI AGGCCT 1 cut(s) 2722
StyD4I CCNGG 6 cut(s) 330, 367, 553, 1447, 1826, 2130
StyI CCWWGG 3 cut(s) 580, 822, 2335
TaaI ACNGT 9 cut(s) 125, 159, 571, 1225, 1389, 1519, 2347, 2458, 2585
TaiI ACGT 5 cut(s) 478, 1610, 1849, 2047, 2576
TasI AATT 9 cut(s) 690, 785, 857, 1217, 2304, 2390, 2412, 2681, 2832
TatI WGTACW 5 cut(s) 571, 798, 915, 1225, 2155
TauI GCSGC 8 cut(s) 236, 367, 542, 617, 773, 1457, 1619, 2501
TfiI GAWTC 8 cut(s) 644, 887, 1522, 1669, 1917, 2230, 2631, 2778
TscAI CASTG 4 cut(s) 574, 763, 974, 1977
TseFI GTSAC 5 cut(s) 476, 1443, 1608, 2202, 2396
TseI GCWGC 8 cut(s) 1249, 1571, 1892, 2315, 2318, 2714, 2846, 2849
Tsp45I GTSAC 5 cut(s) 476, 1443, 1608, 2202, 2396
TspDTI ATGAA 9 cut(s) 314, 457, 743, 1145, 1382, 1737, 1757, 1810, 2208
TspGWI ACGGA 9 cut(s) 47, 77, 401, 1761, 1920, 2057, 2087, 2221, 2300
TspRI CASTG 4 cut(s) 574, 763, 974, 1977
Tth111I GACNNNGTC 3 cut(s) 596, 2594, 2804
Van91I CCANNNNNTGG 1 cut(s) 2240
Vha464I CTTAAG 1 cut(s) 763
VpaK11BI GGWCC 1 cut(s) 2332
VspI ATTAAT 1 cut(s) 1719
XagI CCTNNNNNAGG 1 cut(s) 1035
XapI RAATTY 2 cut(s) 690, 1217
XcmI CCANNNNNNNNNTGG 2 cut(s) 435, 602
XhoI CTCGAG 2 cut(s) 201, 2493
XmiI GTMKAC 1 cut(s) 1677
ZraI GACGTC 2 cut(s) 1608, 1847
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.