Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004385

Physical Location & Seq

Genomic Coordinates Reverse (-)

15376816 .. 15383064

Gene Structure 6249 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1179500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2871 bp

ATGCTTATAAGGACTCCACCTGAGGTTTATAGGCAGAGATTGTTCAAGGGAACATATGTGGATCAATTTGATCTTGTTAGAGAAAACGCTTCCTTCCATGGCCTCAACTCGTCTGGTCGAGTTGTCCTCAAGCGCTGTCACTCGCAGCAGATGCAAATTATAAACGCTATCACAAACCCAAACCCTAAGAAGAACGAGCCAGGACACATTGAAGCAAAAGCTGTTCTGTCCCCAGTTGCAGATCAGACTGCACCAACAACCAGAAAGCGAGTATTTACTTTTGGAAAAGGAAAGACTGAAGGCAACAAGGCCATGAAGTCATTGTTGGGAGGGAAAGGTGCAAACCTGGCAGAAATGGCTAGCATTGGGCTATCCGTCCCACCTGGACTTACTATTTCAACAGAAGCATGCCAAGAGTATCAGCTGAATGGCAAAGATTTACCACAAGGTCTGTGGGACGAGATATTAGAGGGCTTAGAGAGTGTGCAGAAGGACATGGGTGCTATTCTCGGTGACCCTGCCAAGCCTCTCCTCCTCTCCGTTCGCTCTGGTGCTGCGATTTCCATGCCTGGTATGATGGACACTGTCCTCAACCTTGGACTGAATGATGAGGTGGTTGCTGGTTTGGCCGAAAAAAGTGGAGAGCGCTTTGCTTATGACTCGTACAGACGTTTTCTAGACATGTTTGGAGATGTTGTTATGGGAATTCCACACTCATCATTTGAGGAGCAGTTGGAAAAACTAAAAGGTACAAAAGGAGTTGAGCTTGACACAAAGCTAACAGCCTCTGATCTTAAAGAACTGGTAGAACAATACAAAAATGTGTACCTTGAAACCACGGGTGAAAAGTTCCCTTCAGATCCAAAACAACAGCTACTGTTGGCTATCAAAGCAGTTTTTGATTCATGGGACAGTCCAAGGGCCAATAAATACCGGAGCATCAATCAGATAACTGGTTTGAAGGGAACCGCAGTTAACATTCAATGCATGGTTTTCGGGAACATGGGAAATACTTCAGGGACAGGTGTTTTGTTCACTAGGAATCCAAGCACCGGAGAAAGGAAGCTTTATGGCGAATTTTTAATCAATGCTCAGGGAGAAGATGTAGTTGCCGGCATTAGGACGCCAGAAGACTTGGATACTATGAAAAATTGTATGCCAGAAGCTTACGAGGAGCTTGTGGAGAACTGTGAAATTCTAGAAAAGCATTACAAAGATATGATGGACATCGAGTTCACAGTCCAAGAAAATAGGTTGTGGATGTTACAATGCCGCACTGGAAAGCGTACTGGTAAAGGTGCGGTTAAGATAGCTGTAGACATGGTGAACGAAGGGCTTGTGGATAAGCCTGCGGCAATCAAGATGGTGGAACCACAGCACCTTGACCAACTTCTTCACCCACAGTTTGAGAATCCAACCGCTTACAAAGACAAAGTGATCGCCACTGGATTACCGGCATCTCCAGGAGCAGCTGTAGGGACAGTCGTATTCAGTGCTGATGATGCTGAAGCATGGCATGCGCAAGGAAAGAGTGTCATCCTGGTAAGAACGGAGACTAGCCCCGAAGATGTTGGGGGTATGCATGCTGCTGCAGGGATCTTGACAGCTAGAGGTGGCATGACATCTCATGCAGCCGTTGTAGCCCGTGGGTGGGGTAAATGTTGTGTTTCTGGCTGTGCTGACATCCGAGTAAATGACGCTGAGAAGCTGATTGTGATCGGGAATACAGTGGTCAATGAAGGAGAATGGCTTTCACTCAACGGATCCACAGGCGAAGTCATATTAGGAAAACAGCCACTTTCTCCTCCCGCTTTAAGTGGCGATTTGGAGACCTTCATGTCTTGGGCTGATAAAGTCAGGCGTCTCAAGGTTATGGCAAACGCGGACACACCTGAAGATGCGCTAACAGCAAGAAATAACGGTGCGGAAGGGATTGGACTCTGCAGGACAGAGCACATGTTTTTTGCTTCGGATGATAGAATAAAGGCGGTAAGAAAAATGATCATGGCAGCTACAACTGAACAGAGGAAGGCGACACTGAACCTCTTACTACCGTATCAAAGATCTGACTTTGAAGGAATTTTCCGGGCAATGGATGGTCTTCCGGTAACAATCCGCCTGTTAGACCCTCCACTTCATGAATTTCTTCCAGAAGGTGACTTAGAACAGATTGTCGGTGAACTAACTGCAGGGACTGGCATGACTGAGGATGAAGTATTCTCAAGAATTGAGAAATTATCAGAAGTAAACCCCATGCTTGGTTTCCGTGGCTGCAGGTTAGGGATATCATACCCAGAACTCACCGAAATGCAGGCGCGTGCAATCTTTCAAGCTGCAGTCTCAATGAGCAACCAGGGTGTCAAAGTTTTCCCCGAGATAATGGTTCCCCTTGAACTAGGACATCAAGTGAGACTCATTCACAGTGTTGCGGTCAAAGTGTTCTCCGAGATGGGTACTACCTTGAGTTATAAGGTTGGGACTATGATTGAGATCCCTAGAGCTGCTCTGGTTGCAGATGAGATTGCGAAGGAAGCAGAGTTCTTCTCCTTCGGGACCAATGATCTCACACAAATGACATTTGGCTACAGTAGAGATGATGTGGGCAAGTTTCTACCTATCTACCTTGCGAAAGGCCTCCTTCAAAGCGATCCATTTGAGGTACTTGATCAAAGAGGTGTTGGGCAGCTGATCAAGATGGCCACGGAAAAGGGTCGCGCATCTAGGCCTAGCTTAAAGGTTGGAATATGCGGAGAGCATGGTGGGGAGCCTTCTTCTGTTGCATTCTTTGCAGAGGCCGGACTAGACTATGTTTCGTGTTCTCCATTCAGGGTACCTATTGCTAGGCTAGCAGCTGCTCAAGTTGCCGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEP-utilizers PEP-utilizers_C PPDK_N

Protein Analysis

957

Amino Acids

104.16

Weight (kDa)

5.49

Isoelectric Point (pI)

33.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPDK_N	PF01326	107 - 163	4.1e-07	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N	PF01326	145 - 379	7.5e-23	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N	PF01326	389 - 444	1.1e-10	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PEP-utilizers	PF00391	510 - 591	1e-22	PEP-utilising enzyme, mobile domain
PEP-utilizers_C	PF02896	606 - 955	4e-89	PEP-utilising enzyme, PEP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004385)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530
fragaria_vesca	FvH4_4g16670
malus_domestica	MD13G1177400.v1.1 MD13G1177500.v1.1 MD16G1179300.v1.1 MD16G1179400.v1.1 MD16G1179500.v1.1
prunus_persica	Prupe.1G146600_v2.0.a1
pyrus_communis	pycom13g15300 pycom16g15050 pycom16g15060 pycom16g15070
rosa_chinensis	RchiOBHm_Chr4g0420131
rosa_laevigata	RLG00000007786
rosa_multiflora	Rmu_sc0001252.1_g000003 Rmu_sc0025222.1_g000001
rosa_roxburghii	Rroxscaffold_5G00362840
rosa_rugosa	Rorug04G0162400 Rorug04G0162500 Rorug04G0162600 Rorug04G0162600
rosa_samantha	Rh4AG223300 Rh4BG225700 Rh4CG236500 Rh4DG222100
rosa_wichuraiana	Rw4G019220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	8, 161, 2469
AasI	GACNNNNNNGTC	1 cut(s)	1837
Acc16I	TGCGCA	1 cut(s)	1521
Acc36I	ACCTGC	1 cut(s)	2265
Acc65I	GGTACC	1 cut(s)	2830
AccB1I	GGYRCC	1 cut(s)	2830
AccI	GTMKAC	1 cut(s)	1317
AccII	CGCG	3 cut(s)	1883, 2317, 2715
AclWI	GGATC	7 cut(s)	69, 854, 1604, 1758, 1771, 2485, 2642
AcoI	YGGCCR	2 cut(s)	627, 2697
AcsI	RAATTY	5 cut(s)	705, 1076, 1194, 2079, 2141
AcuI	CTGAAG	5 cut(s)	318, 840, 999, 1527, 1914
AcyI	GRCGYC	2 cut(s)	1124, 1861
AfaI	GTAC	7 cut(s)	665, 751, 827, 1288, 2455, 2661, 2832
AfeI	AGCGCT	2 cut(s)	134, 647
AfiI	CCNNNNNNNGG	7 cut(s)	1052, 1059, 1119, 1650, 1651, 2092, 2258
AflIII	ACRYGT	2 cut(s)	681, 1956
AjnI	CCWGG	7 cut(s)	199, 345, 382, 568, 1462, 1539, 2352
AjuI	GAANNNNNNNTTGG	2 cut(s)	308, 340
AloI	GAACNNNNNNTCC	4 cut(s)	1217, 1249, 2522, 2554
Alw21I	GWGCWC	1 cut(s)	1956
Alw26I	GTCTC	5 cut(s)	1547, 1823, 1868, 2344, 2404
AlwI	GGATC	7 cut(s)	69, 854, 1604, 1758, 1771, 2485, 2642
AlwNI	CAGNNNCTG	3 cut(s)	788, 877, 2195
Ama87I	CYCGRG	1 cut(s)	2372
Aor51HI	AGCGCT	2 cut(s)	134, 647
AoxI	GGCC	8 cut(s)	100, 309, 627, 921, 2632, 2697, 2723, 2793
ApoI	RAATTY	5 cut(s)	705, 1076, 1194, 2079, 2141
ArsI	GACNNNNNNTTYG	2 cut(s)	1012, 1044
Asp700I	GAANNNNTTC	2 cut(s)	1012, 2145
Asp718I	GGTACC	1 cut(s)	2830
AspLEI	GCGC	6 cut(s)	135, 648, 1522, 1903, 2317, 2717
AspS9I	GGNCC	2 cut(s)	921, 2553
AsuC2I	CCSGG	1 cut(s)	2087
AsuHPI	GGTGA	7 cut(s)	524, 854, 1336, 1388, 2168, 2189, 2293
AsuNHI	GCTAGC	2 cut(s)	359, 2845
AvaI	CYCGRG	1 cut(s)	2372
AvaII	GGWCC	1 cut(s)	2553
AxyI	CCTNAGG	1 cut(s)	21
BaeI	ACNNNNGTAYC	2 cut(s)	2039, 2072
BalI	TGGCCA	1 cut(s)	2699
BamHI	GGATCC	1 cut(s)	1763
BanI	GGYRCC	1 cut(s)	2830
BbsI	GAAGAC	2 cut(s)	1137, 2093
Bbv12I	GWGCWC	1 cut(s)	1956
BccI	CCATC	6 cut(s)	571, 1216, 1357, 2090, 2443, 2689
BceAI	ACGGC	2 cut(s)	1619, 2849
BcgI	CGANNNNNNTGC	4 cut(s)	547, 581, 976, 1010
BciT130I	CCWGG	7 cut(s)	201, 347, 384, 570, 1464, 1541, 2354
BciVI	GTATCC	1 cut(s)	1132
BclI	TGATCA	3 cut(s)	2001, 2665, 2688
BcnI	CCSGG	1 cut(s)	2087
BcoDI	GTCTC	5 cut(s)	1547, 1823, 1868, 2344, 2404
BfmI	CTRYAG	8 cut(s)	1314, 1473, 1590, 1942, 2187, 2272, 2334, 2584
BfoI	RGCGCY	2 cut(s)	136, 649
BfuAI	ACCTGC	1 cut(s)	2265
BfuI	GTATCC	1 cut(s)	1132
BglII	AGATCT	1 cut(s)	2063
Bme1390I	CCNGG	8 cut(s)	201, 347, 384, 570, 1464, 1541, 2087, 2354
Bme18I	GGWCC	1 cut(s)	2553
BmeT110I	CYCGRG	1 cut(s)	2372
BmgT120I	GGNCC	2 cut(s)	921, 2553
BmiI	GGNNCC	7 cut(s)	967, 1371, 1765, 2385, 2554, 2766, 2832
BmrFI	CCNGG	8 cut(s)	201, 347, 384, 570, 1464, 1541, 2087, 2354
BmrI	ACTGGG	1 cut(s)	227
BmsI	GCATC	6 cut(s)	141, 948, 1466, 1492, 1888, 2726
BmtI	GCTAGC	2 cut(s)	363, 2849
BmuI	ACTGGG	1 cut(s)	227
BpiI	GAAGAC	2 cut(s)	1137, 2093
BplI	GAGNNNNNCTC	4 cut(s)	111, 143, 2528, 2560
BpmI	CTGGAG	1 cut(s)	1446
Bpu10I	CCTNAGC	1 cut(s)	1092
BpuEI	CTTGAG	5 cut(s)	113, 1850, 2206, 2482, 2841
BpuMI	CCSGG	1 cut(s)	2087
BsaBI	GATNNNNATC	3 cut(s)	1226, 1715, 2489
BsaHI	GRCGYC	2 cut(s)	1124, 1861
BsaI	GGTCTC	1 cut(s)	1823
BsaJI	CCNNGG	8 cut(s)	97, 595, 837, 917, 1645, 2266, 2353, 2700
BsaWI	WCCGGW	3 cut(s)	933, 1052, 2104
BsaXI	ACNNNNNCTCC	6 cut(s)	681, 711, 1089, 1119, 1458, 1488
Bsc4I	CCNNNNNNNGG	7 cut(s)	1052, 1059, 1119, 1650, 1651, 2092, 2258
Bse118I	RCCGGY	2 cut(s)	1112, 1453
Bse1I	ACTGG	7 cut(s)	233, 807, 958, 1282, 1294, 1450, 2200
Bse21I	CCTNAGG	1 cut(s)	21
Bse3DI	GCAATG	1 cut(s)	2097
Bse8I	GATNNNNATC	3 cut(s)	1226, 1715, 2489
BseBI	CCWGG	7 cut(s)	201, 347, 384, 570, 1464, 1541, 2354
BseDI	CCNNGG	8 cut(s)	97, 595, 837, 917, 1645, 2266, 2353, 2700
BseGI	GGATG	6 cut(s)	1266, 1536, 1683, 1978, 2101, 2215
BseJI	GATNNNNATC	3 cut(s)	1226, 1715, 2489
BseLI	CCNNNNNNNGG	7 cut(s)	1052, 1059, 1119, 1650, 1651, 2092, 2258
BseMI	GCAATG	1 cut(s)	2097
BseMII	CTCAG	4 cut(s)	12, 1106, 1692, 2196
BseNI	ACTGG	7 cut(s)	233, 807, 958, 1282, 1294, 1450, 2200
BseRI	GAGGAG	5 cut(s)	521, 524, 740, 1187, 1794
BsgI	GTGCAG	2 cut(s)	234, 506
Bsh1236I	CGCG	3 cut(s)	1883, 2317, 2715
BshFI	GGCC	8 cut(s)	102, 311, 629, 923, 2634, 2699, 2725, 2795
BshNI	GGYRCC	1 cut(s)	2830
BsiHKAI	GWGCWC	1 cut(s)	1956
BsiHKCI	CYCGRG	1 cut(s)	2372
BsiSI	CCGG	7 cut(s)	934, 1053, 1113, 1454, 2086, 2105, 2796
BslFI	GGGAC	9 cut(s)	214, 362, 470, 923, 1033, 1492, 2206, 2491, 2566
BslI	CCNNNNNNNGG	7 cut(s)	1052, 1059, 1119, 1650, 1651, 2092, 2258
BsmAI	GTCTC	5 cut(s)	1547, 1823, 1868, 2344, 2404
BsmBI	CGTCTC	1 cut(s)	1868
BsmFI	GGGAC	9 cut(s)	214, 362, 470, 923, 1033, 1492, 2206, 2491, 2566
BsmI	GAATGC	1 cut(s)	2780
BsnI	GGCC	8 cut(s)	102, 311, 629, 923, 2634, 2699, 2725, 2795
Bso31I	GGTCTC	1 cut(s)	1823
BsoBI	CYCGRG	1 cut(s)	2372
Bsp1286I	GDGCHC	1 cut(s)	1956
Bsp19I	CCATGG	1 cut(s)	97
BspANI	GGCC	8 cut(s)	102, 311, 629, 923, 2634, 2699, 2725, 2795
BspCNI	CTCAG	4 cut(s)	13, 1105, 1693, 2197
BspFNI	CGCG	3 cut(s)	1883, 2317, 2715
BspHI	TCATGA	1 cut(s)	2137
BspLI	GGNNCC	7 cut(s)	967, 1371, 1765, 2385, 2554, 2766, 2832
BspMAI	CTGCAG	5 cut(s)	1594, 1946, 2191, 2276, 2338
BspMI	ACCTGC	1 cut(s)	2265
BspOI	GCTAGC	2 cut(s)	363, 2849
BspPI	GGATC	7 cut(s)	69, 854, 1604, 1758, 1771, 2485, 2642
BspT107I	GGYRCC	1 cut(s)	2830
BspTNI	GGTCTC	1 cut(s)	1823
BsrDI	GCAATG	1 cut(s)	2097
BsrFI	RCCGGY	2 cut(s)	1112, 1453
BsrI	ACTGG	7 cut(s)	233, 807, 958, 1282, 1294, 1450, 2200
BssAI	RCCGGY	2 cut(s)	1112, 1453
BssECI	CCNNGG	8 cut(s)	97, 595, 837, 917, 1645, 2266, 2353, 2700
BssNI	GRCGYC	2 cut(s)	1124, 1861
BssT1I	CCWWGG	3 cut(s)	97, 595, 917
Bst2UI	CCWGG	7 cut(s)	201, 347, 384, 570, 1464, 1541, 2354
BstACI	GRCGYC	2 cut(s)	1124, 1861
BstAPI	GCANNNNNTGC	3 cut(s)	151, 1517, 2786
BstC8I	GCNNGC	9 cut(s)	361, 409, 1114, 1350, 1518, 1584, 2313, 2319, 2847
BstDEI	CTNAG	7 cut(s)	21, 186, 475, 1092, 1701, 2161, 2205
BstDSI	CCRYGG	5 cut(s)	97, 837, 1645, 2266, 2700
BstEII	GGTNACC	1 cut(s)	512
BstF5I	GGATG	6 cut(s)	1266, 1536, 1683, 1978, 2101, 2215
BstFNI	CGCG	3 cut(s)	1883, 2317, 2715
BstH2I	RGCGCY	2 cut(s)	136, 649
BstHHI	GCGC	6 cut(s)	135, 648, 1522, 1903, 2317, 2717
BstMAI	GTCTC	5 cut(s)	1547, 1823, 1868, 2344, 2404
BstNI	CCWGG	7 cut(s)	201, 347, 384, 570, 1464, 1541, 2354
BstNSI	RCATGY	5 cut(s)	411, 685, 1520, 1586, 1960
BstPI	GGTNACC	1 cut(s)	512
BstSCI	CCNGG	8 cut(s)	199, 345, 382, 568, 1462, 1539, 2085, 2352
BstSFI	CTRYAG	8 cut(s)	1314, 1473, 1590, 1942, 2187, 2272, 2334, 2584
BstUI	CGCG	3 cut(s)	1883, 2317, 2715
BstV2I	GAAGAC	2 cut(s)	1137, 2093
BstX2I	RGATCY	5 cut(s)	859, 1596, 1763, 2063, 2490
BstYI	RGATCY	5 cut(s)	859, 1596, 1763, 2063, 2490
Bsu36I	CCTNAGG	1 cut(s)	21
BsuI	GTATCC	1 cut(s)	1132
BsuRI	GGCC	8 cut(s)	102, 311, 629, 923, 2634, 2699, 2725, 2795
BtgI	CCRYGG	5 cut(s)	97, 837, 1645, 2266, 2700
BtsCI	GGATG	6 cut(s)	1266, 1536, 1683, 1978, 2101, 2215
BtsIMutI	CAGTG	7 cut(s)	582, 1275, 1443, 1498, 1734, 2036, 2428
BveI	ACCTGC	1 cut(s)	2265
Cac8I	GCNNGC	9 cut(s)	361, 409, 1114, 1350, 1518, 1584, 2313, 2319, 2847
CaiI	CAGNNNCTG	3 cut(s)	788, 877, 2195
CciI	TCATGA	1 cut(s)	2137
CfoI	GCGC	6 cut(s)	135, 648, 1522, 1903, 2317, 2717
Cfr10I	RCCGGY	2 cut(s)	1112, 1453
Cfr13I	GGNCC	2 cut(s)	921, 2553
CseI	GACGC	3 cut(s)	1132, 1706, 1850
Csp6I	GTAC	7 cut(s)	664, 750, 826, 1287, 2454, 2660, 2831
CspCI	CAANNNNNGTGG	2 cut(s)	434, 469
CviQI	GTAC	7 cut(s)	664, 750, 826, 1287, 2454, 2660, 2831
DdeI	CTNAG	7 cut(s)	21, 186, 475, 1092, 1701, 2161, 2205
DrdI	GACNNNNNNGTC	1 cut(s)	1837
DseDI	GACNNNNNNGTC	1 cut(s)	1837
EaeI	YGGCCR	2 cut(s)	627, 2697
EciI	GGCGGA	1 cut(s)	2105
Eco130I	CCWWGG	3 cut(s)	97, 595, 917
Eco147I	AGGCCT	2 cut(s)	2634, 2725
Eco31I	GGTCTC	1 cut(s)	1823
Eco32I	GATATC	1 cut(s)	2286
Eco47I	GGWCC	1 cut(s)	2553
Eco47III	AGCGCT	2 cut(s)	134, 647
Eco57I	CTGAAG	5 cut(s)	318, 840, 999, 1527, 1914
Eco81I	CCTNAGG	1 cut(s)	21
Eco88I	CYCGRG	1 cut(s)	2372
Eco91I	GGTNACC	1 cut(s)	512
EcoO65I	GGTNACC	1 cut(s)	512
EcoRI	GAATTC	1 cut(s)	705
EcoRII	CCWGG	7 cut(s)	199, 345, 382, 568, 1462, 1539, 2352
EcoRV	GATATC	1 cut(s)	2286
EcoT14I	CCWWGG	3 cut(s)	97, 595, 917
EcoT22I	ATGCAT	2 cut(s)	989, 1584
ErhI	CCWWGG	3 cut(s)	97, 595, 917
Esp3I	CGTCTC	1 cut(s)	1868
FalI	AAGNNNNNCTT	2 cut(s)	2622, 2654
FaqI	GGGAC	9 cut(s)	214, 362, 470, 923, 1033, 1492, 2206, 2491, 2566
FauI	CCCGC	1 cut(s)	1816
FauNDI	CATATG	1 cut(s)	55
FbaI	TGATCA	3 cut(s)	2001, 2665, 2688
FblI	GTMKAC	1 cut(s)	1317
FokI	GGATG	6 cut(s)	1273, 1523, 1670, 1985, 2108, 2222
FspI	TGCGCA	1 cut(s)	1521
GlaI	GCGC	6 cut(s)	134, 647, 1521, 1902, 2316, 2716
GsuI	CTGGAG	1 cut(s)	1446
HaeII	RGCGCY	2 cut(s)	136, 649
HaeIII	GGCC	8 cut(s)	102, 311, 629, 923, 2634, 2699, 2725, 2795
HapII	CCGG	7 cut(s)	934, 1053, 1113, 1454, 2086, 2105, 2796
HgaI	GACGC	3 cut(s)	1132, 1706, 1850
HhaI	GCGC	6 cut(s)	135, 648, 1522, 1903, 2317, 2717
Hin1I	GRCGYC	2 cut(s)	1124, 1861
Hin6I	GCGC	6 cut(s)	133, 646, 1520, 1901, 2315, 2715
HinP1I	GCGC	6 cut(s)	133, 646, 1520, 1901, 2315, 2715
HincII	GTYRAC	1 cut(s)	976
HindII	GTYRAC	1 cut(s)	976
HindIII	AAGCTT	2 cut(s)	1064, 1164
HinfI	GANTC	7 cut(s)	13, 659, 902, 1042, 1411, 1938, 2412
HpaI	GTTAAC	1 cut(s)	976
HpaII	CCGG	7 cut(s)	934, 1053, 1113, 1454, 2086, 2105, 2796
HphI	GGTGA	7 cut(s)	524, 854, 1336, 1388, 2168, 2189, 2293
Hpy166II	GTNNAC	8 cut(s)	826, 976, 1035, 1236, 1318, 1327, 2180, 2248
Hpy188I	TCNGA	9 cut(s)	246, 790, 859, 948, 1688, 1972, 2068, 2242, 2446
Hpy8I	GTNNAC	8 cut(s)	826, 976, 1035, 1236, 1318, 1327, 2180, 2248
HpyCH4IV	ACGT	1 cut(s)	670
HpyF3I	CTNAG	7 cut(s)	21, 186, 475, 1092, 1701, 2161, 2205
HpySE526I	ACGT	1 cut(s)	670
Hsp92I	GRCGYC	2 cut(s)	1124, 1861
HspAI	GCGC	6 cut(s)	133, 646, 1520, 1901, 2315, 2715
KpnI	GGTACC	1 cut(s)	2834
KroI	GCCGGC	1 cut(s)	1112
KroNI	GCCGGC	1 cut(s)	1114
Ksp22I	TGATCA	3 cut(s)	2001, 2665, 2688
KspAI	GTTAAC	1 cut(s)	976
LmnI	GCTCC	5 cut(s)	727, 936, 1174, 1466, 2764
LweI	GCATC	6 cut(s)	141, 948, 1466, 1492, 1888, 2726
MaeII	ACGT	1 cut(s)	670
MaeIII	GTNAC	5 cut(s)	137, 512, 1263, 2107, 2156
MflI	RGATCY	5 cut(s)	859, 1596, 1763, 2063, 2490
MhlI	GDGCHC	1 cut(s)	1956
MlsI	TGGCCA	1 cut(s)	2699
MluNI	TGGCCA	1 cut(s)	2699
MlyI	GAGTC	4 cut(s)	7, 653, 1932, 2406
MmeI	TCCRAC	3 cut(s)	714, 1439, 2719
Mox20I	TGGCCA	1 cut(s)	2699
Mph1103I	ATGCAT	2 cut(s)	989, 1584
MroNI	GCCGGC	1 cut(s)	1112
MroXI	GAANNNNTTC	2 cut(s)	1012, 2145
MscI	TGGCCA	1 cut(s)	2699
MseI	TTAA	6 cut(s)	795, 975, 1082, 1305, 1814, 2732
MslI	CAYNNNNRTG	2 cut(s)	2306, 2570
Msp20I	TGGCCA	1 cut(s)	2699
MspA1I	CMGCKG	4 cut(s)	424, 1472, 2686, 2852
MspI	CCGG	7 cut(s)	934, 1053, 1113, 1454, 2086, 2105, 2796
MspR9I	CCNGG	8 cut(s)	201, 347, 384, 570, 1464, 1541, 2087, 2354
Mva1269I	GAATGC	1 cut(s)	2780
MvaI	CCWGG	7 cut(s)	201, 347, 384, 570, 1464, 1541, 2354
MvnI	CGCG	3 cut(s)	1883, 2317, 2715
NaeI	GCCGGC	1 cut(s)	1114
NciI	CCSGG	1 cut(s)	2087
NcoI	CCATGG	1 cut(s)	97
NdeI	CATATG	1 cut(s)	55
NgoMIV	GCCGGC	1 cut(s)	1112
NheI	GCTAGC	2 cut(s)	359, 2845
NlaIV	GGNNCC	7 cut(s)	967, 1371, 1765, 2385, 2554, 2766, 2832
NmuCI	GTSAC	3 cut(s)	137, 512, 2156
NsbI	TGCGCA	1 cut(s)	1521
NsiI	ATGCAT	2 cut(s)	989, 1584
NspI	RCATGY	5 cut(s)	411, 685, 1520, 1586, 1960
PaeI	GCATGC	3 cut(s)	411, 1520, 1586
PagI	TCATGA	1 cut(s)	2137
PceI	AGGCCT	2 cut(s)	2634, 2725
PciI	ACATGT	2 cut(s)	681, 1956
PctI	GAATGC	1 cut(s)	2780
PdiI	GCCGGC	1 cut(s)	1114
PdmI	GAANNNNTTC	2 cut(s)	1012, 2145
PfeI	GAWTC	3 cut(s)	902, 1042, 1411
PflFI	GACNNNGTC	1 cut(s)	584
PfoI	TCCNGGA	1 cut(s)	1462
PleI	GAGTC	4 cut(s)	7, 653, 1932, 2406
PpsI	GAGTC	4 cut(s)	7, 653, 1932, 2406
PscI	ACATGT	2 cut(s)	681, 1956
PsiI	TTATAA	3 cut(s)	8, 161, 2469
Psp6I	CCWGG	7 cut(s)	199, 345, 382, 568, 1462, 1539, 2352
PspEI	GGTNACC	1 cut(s)	512
PspGI	CCWGG	7 cut(s)	199, 345, 382, 568, 1462, 1539, 2352
PspN4I	GGNNCC	7 cut(s)	967, 1371, 1765, 2385, 2554, 2766, 2832
PspPI	GGNCC	2 cut(s)	921, 2553
PstI	CTGCAG	5 cut(s)	1594, 1946, 2191, 2276, 2338
PstNI	CAGNNNCTG	3 cut(s)	788, 877, 2195
PsuI	RGATCY	5 cut(s)	859, 1596, 1763, 2063, 2490
PsyI	GACNNNGTC	1 cut(s)	584
PvuII	CAGCTG	4 cut(s)	424, 1472, 2686, 2852
RsaI	GTAC	7 cut(s)	665, 751, 827, 1288, 2455, 2661, 2832
RsaNI	GTAC	7 cut(s)	664, 750, 826, 1287, 2454, 2660, 2831
RseI	CAYNNNNRTG	2 cut(s)	2306, 2570
SaqAI	TTAA	6 cut(s)	795, 975, 1082, 1305, 1814, 2732
Sau96I	GGNCC	2 cut(s)	921, 2553
SchI	GAGTC	4 cut(s)	7, 653, 1932, 2406
ScrFI	CCNGG	8 cut(s)	201, 347, 384, 570, 1464, 1541, 2087, 2354
SduI	GDGCHC	1 cut(s)	1956
SfaNI	GCATC	6 cut(s)	141, 948, 1466, 1492, 1888, 2726
SfcI	CTRYAG	8 cut(s)	1314, 1473, 1590, 1942, 2187, 2272, 2334, 2584
SinI	GGWCC	1 cut(s)	2553
SmiMI	CAYNNNNRTG	2 cut(s)	2306, 2570
SmlI	CTYRAG	5 cut(s)	128, 1865, 2221, 2461, 2856
SmoI	CTYRAG	5 cut(s)	128, 1865, 2221, 2461, 2856
SphI	GCATGC	3 cut(s)	411, 1520, 1586
SseBI	AGGCCT	2 cut(s)	2634, 2725
StuI	AGGCCT	2 cut(s)	2634, 2725
StyD4I	CCNGG	8 cut(s)	199, 345, 382, 568, 1462, 1539, 2085, 2352
StyI	CCWWGG	3 cut(s)	97, 595, 917
TaiI	ACGT	1 cut(s)	673
TaqI	TCGA	2 cut(s)	118, 1230
TauI	GCSGC	2 cut(s)	1275, 1355
TfiI	GAWTC	3 cut(s)	902, 1042, 1411
Tru1I	TTAA	6 cut(s)	795, 975, 1082, 1305, 1814, 2732
Tru9I	TTAA	6 cut(s)	795, 975, 1082, 1305, 1814, 2732
TscAI	CASTG	7 cut(s)	589, 1282, 1450, 1498, 1734, 2043, 2428
TseFI	GTSAC	3 cut(s)	137, 512, 2156
Tsp45I	GTSAC	3 cut(s)	137, 512, 2156
TspDTI	ATGAA	8 cut(s)	329, 894, 1160, 1752, 1825, 2126, 2154, 2226
TspGWI	ACGGA	6 cut(s)	364, 529, 1565, 1776, 2255, 2717
TspRI	CASTG	7 cut(s)	589, 1282, 1450, 1498, 1734, 2043, 2428
Tth111I	GACNNNGTC	1 cut(s)	584
VpaK11BI	GGWCC	1 cut(s)	2553
XapI	RAATTY	5 cut(s)	705, 1076, 1194, 2079, 2141
XbaI	TCTAGA	2 cut(s)	676, 1198
XceI	RCATGY	5 cut(s)	411, 685, 1520, 1586, 1960
XcmI	CCANNNNNNNNNTGG	1 cut(s)	450
XmiI	GTMKAC	1 cut(s)	1317
XmnI	GAANNNNTTC	2 cut(s)	1012, 2145
Zsp2I	ATGCAT	2 cut(s)	989, 1584

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.