Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004385

Physical Location & Seq

Genomic Coordinates Reverse (-)

15347105 .. 15351955

Gene Structure 4851 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1179300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2574 bp

ATGAAGTCATTGTTGGGAGGGAAAGGTGCAAACCTGGCAGAAATGGCTAGCATTGGGCTATCCGTCCCGCCTGGACTTACTATATCAACAGAAGCATGCCAAGAGTATCAACTCAATGGCAAAGACTTACCACAAGGTTTATGGGAGGAGATATTAGAGGGCTTGGAGATTGTGCAGAAGGACATTGGTGCTATTCTTGGTGATCCTGCAAAGCCTCTTCTTGTCTCTGTTCGCTCTGGTGCCGCAATTTCCATGCCTGGCATGATGGACACTGTCCTCAACCTTGGACTCAATGACAAGGTGGTTGCTGGTTTGGCCGCAAAAAGTGGAGAGCGCTTTGCTTATGACTCGTACAGACGTTTCCTAGACATGTTTGGAGATGTTGTTATGGGAATTCCACACTCATCGTTCGAAGAGAAATTGGAAAAACTAAAAGGTAAAAAAGGAGTTGAGCTTGATACAAAGCTAACAGCTTCTGATCTTAAAGAGCTGGTAGACCAATACAAAAATGTCTATCTCGAAACGACGGGTGAAAAGTTCCCTTCAGATCCAAAACAACAGCTCCTCTTGGCTGTTAAAGCAGTTTTTGATTCTTGGGACAGCTCAAGGGCCATTAAATACCGGAGCATCAATCAGATAACTGGTTTGAAGGGAACCGCGGTTAACATTCAATGCATGGTTTTCGGGAACATGGGAAATACTTCAGGGACAGGTGTTTTGTTCACTAGGAATCCAAGCACCGGAGAAAGGAAGCTTTATGGCGAATTTTTAATCAATGCTCAGGGAGAAGATGTAGTTGCCGGGATCAGGACACCAGAAGACGTGGATGCTATGAAAAATTGCATGCCGGAAGCTTACAAGGAGCTTGTGGAGAACTGTGAAATTCTAGAAAAACATTACAAAGATATGATGGACATTGAGTTCACAGTCCAAGAAAATAGGTTGTGGATGTTACAATGCCGCGTTGGAAAGCGTACTGGCAAAGGAGCAGTTAAGATAGCTGTAGACATGGCTAACGAAGGGCTTGTGAATAAGCATGCTGCAATCAAGATGGTGGAACCACAGCACCTTGACCAACTTCTTCATCCACAGTTTGAGAATCCAACGGCTTACAAAGACAAAGTGCTCGCCACTGGGTTACCTGCATCTCCAGGAGCAGCAGTAGGACAAGTCGTGTTCAATGCAGAAGATGCTGAATCATGGCATGCACAAGGAAAGAAAGTTATCCTGGTAAGGACTGAAACTAGCCCCGAAGATGTTGGGGGTATGCATGCTGCTGCTGGAATCTTGACAGCTAGAGGTGGCATGACATCTCATGCGGCTGTTGTAGCCCGTGGGTGGGGTAAATGTTGTGTTTCTGGCTGCGCTGACATCCGAGTAAATGACAGAGAGAAGCTGGTTTTGATCGGGAAAACGGTGGTCAATGAAGGAGAATGGCTTTCACTCAATGGATCCACTGGTGAAGTCATATTGGGAAAACAGCCACTTTCTCCTCCTGCTTTGAGTGGCGATTTGGAAACCTTTATGTCTTGGGCTGATAAAGTCAGGCGTCTCAAGGTGATGGCAAATGCTGACACGCCTGAAGATGCGCTAACAGCAAGAAATAACGGTGCACAAGGGATTGGACTTTGCAGGACAGAACACATGTTCTTTGCTTCGGATGATAGAATAAAGGCAGTAAGAAAAATGATCATGGCGGCTACACCTGAACAGAGGAAGGCAGCAATGGACCTCTTACTTCCATATCAGAGATCTGACTTTGAAGGAATTTTCCGTGCAATGGATGGTCTTCCGGTAACAATCCGCCTGTTAGACCCTCCACTGCATGAATTTCTTCCAGAAGGTAACTTGGAGCAGATTGTTGGCCAACTAACTACACAGACTGGCATGACTGAGGATGAAGTTTTCTCCAGAGTTGAAAAATTATCAGAAGTGAACCCCATGCTTGGTTTCCGCGGGTGCAGGCTAGGGATATCCTACCCAGAGCTCACCGAAATGCAGGCATGCGCAATCTTTCAAGCTGCAGTCTCAATGAGCAACCAGGGTGTCAAAGTTTTCCCTGAGATAATGGTTCCCCTTGAACTGGGTCATCAAGTGAAGCTCATTCGCAGTGTTGCGGTTAACGTGTTCTCTGAGATGGGTACCACCGTGAGCTATAAGGTTGGAACTATGATCGAGATCCCTAGAGCTGCTCTGGTTGCAGATGAGATTGCAAAGGAAGCTGAGTTCTTCTCCTTCGGGACCAATGATCTCACACAAATGACATTCGGGTACAGTAGAGATGATGTGGGCAAGTTTCTACCTATTTACCTCTCCAAAGGCCTTCTTCAAAACGATCCCTTCCAGGTACTTGATCAACAAGGCGTTGGGCAGCTCGTCAAGATGGCAACGGAAAAGGGTCGTGCAGCTAGGCCTAGCTTAAAGGTTGGAATATGCGGAGAGCATGGTGGAGAGCCTTCTTCTGTTGCATTTTTTGCAGAGGCCGGACTAGACTACGTTTCATGTTCTCCATTAAGGGTACCTATTGCTAGGCTAGCAGCAGCTCAAGTTGCTGTCTCAGAAAGATCTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEP-utilizers PEP-utilizers_C PPDK_N

Protein Analysis

858

Amino Acids

93.08

Weight (kDa)

5.48

Isoelectric Point (pI)

33.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPDK_N	PF01326	3 - 51	8e-07	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N	PF01326	41 - 276	1.8e-23	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N	PF01326	285 - 340	1.9e-10	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PEP-utilizers	PF00391	406 - 487	4.7e-23	PEP-utilising enzyme, mobile domain
PEP-utilizers_C	PF02896	501 - 851	4.4e-87	PEP-utilising enzyme, PEP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004385)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530
fragaria_vesca	FvH4_4g16670
malus_domestica	MD13G1177400.v1.1 MD13G1177500.v1.1 MD16G1179300.v1.1 MD16G1179400.v1.1 MD16G1179500.v1.1
prunus_persica	Prupe.1G146600_v2.0.a1
pyrus_communis	pycom13g15300 pycom16g15050 pycom16g15060 pycom16g15070
rosa_chinensis	RchiOBHm_Chr4g0420131
rosa_laevigata	RLG00000007786
rosa_multiflora	Rmu_sc0001252.1_g000003 Rmu_sc0025222.1_g000001
rosa_roxburghii	Rroxscaffold_5G00362840
rosa_rugosa	Rorug04G0162400 Rorug04G0162500 Rorug04G0162600 Rorug04G0162600
rosa_samantha	Rh4AG223300 Rh4BG225700 Rh4CG236500 Rh4DG222100
rosa_wichuraiana	Rw4G019220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2008
Acc36I	ACCTGC	1 cut(s)	1150
Acc65I	GGTACC	2 cut(s)	2141, 2518
AccB1I	GGYRCC	3 cut(s)	239, 2141, 2518
AccI	GTMKAC	2 cut(s)	495, 1005
AccII	CGCG	3 cut(s)	659, 963, 1956
AclWI	GGATC	7 cut(s)	197, 542, 812, 1446, 1459, 2173, 2330
AcoI	YGGCCR	2 cut(s)	315, 1864
AcsI	RAATTY	5 cut(s)	393, 764, 882, 1767, 1829
AcuI	CTGAAG	3 cut(s)	528, 687, 1602
AcyI	GRCGYC	1 cut(s)	1549
AfaI	GTAC	6 cut(s)	353, 976, 2143, 2273, 2349, 2520
AfeI	AGCGCT	1 cut(s)	335
AfiI	CCNNNNNNNGG	8 cut(s)	740, 747, 807, 1338, 1339, 1780, 1946, 2083
AflIII	ACRYGT	3 cut(s)	369, 1644, 2124
AgsI	TTSAA	8 cut(s)	649, 671, 1180, 1763, 1919, 2018, 2081, 2330
AjiI	CACGTC	1 cut(s)	823
AjnI	CCWGG	7 cut(s)	33, 70, 256, 1150, 1227, 2040, 2343
AjuI	GAANNNNNNNTTGG	1 cut(s)	28
AloI	GAACNNNNNNTCC	4 cut(s)	905, 937, 2210, 2242
Alw21I	GWGCWC	3 cut(s)	1128, 1615, 1989
Alw26I	GTCTC	4 cut(s)	229, 1556, 2032, 2560
Alw44I	GTGCAC	1 cut(s)	1611
AlwI	GGATC	7 cut(s)	197, 542, 812, 1446, 1459, 2173, 2330
AlwNI	CAGNNNCTG	1 cut(s)	476
Aor51HI	AGCGCT	1 cut(s)	335
AoxI	GGCC	6 cut(s)	315, 609, 1864, 2320, 2411, 2481
ApaLI	GTGCAC	1 cut(s)	1611
ApoI	RAATTY	5 cut(s)	393, 764, 882, 1767, 1829
ArsI	GACNNNNNNTTYG	2 cut(s)	700, 732
Asp700I	GAANNNNTTC	2 cut(s)	700, 1833
Asp718I	GGTACC	2 cut(s)	2141, 2518
AspLEI	GCGC	4 cut(s)	336, 1367, 1591, 2009
AspS9I	GGNCC	3 cut(s)	609, 1729, 2241
AsuC2I	CCSGG	1 cut(s)	802
AsuHPI	GGTGA	5 cut(s)	212, 542, 1472, 1570, 1981
AsuII	TTCGAA	1 cut(s)	411
AsuNHI	GCTAGC	2 cut(s)	47, 2533
AvaII	GGWCC	2 cut(s)	1729, 2241
BaeGI	GKGCMC	1 cut(s)	1615
BaeI	ACNNNNGTAYC	2 cut(s)	2133, 2166
BalI	TGGCCA	1 cut(s)	1866
BamHI	GGATCC	1 cut(s)	1451
BanI	GGYRCC	3 cut(s)	239, 2141, 2518
BanII	GRGCYC	1 cut(s)	1989
BbsI	GAAGAC	2 cut(s)	825, 1781
Bbv12I	GWGCWC	3 cut(s)	1128, 1615, 1989
BccI	CCATC	7 cut(s)	259, 904, 1045, 1555, 1778, 2131, 2377
BceAI	ACGGC	1 cut(s)	1122
BcgI	CGANNNNNNTGC	2 cut(s)	664, 698
BciT130I	CCWGG	7 cut(s)	35, 72, 258, 1152, 1229, 2042, 2345
BclI	TGATCA	2 cut(s)	1689, 2353
BcnI	CCSGG	1 cut(s)	802
BcoDI	GTCTC	4 cut(s)	229, 1556, 2032, 2560
BfmI	CTRYAG	2 cut(s)	1002, 2022
BfoI	RGCGCY	1 cut(s)	337
BfuAI	ACCTGC	1 cut(s)	1150
BglII	AGATCT	2 cut(s)	1751, 2564
Bme1390I	CCNGG	8 cut(s)	35, 72, 258, 802, 1152, 1229, 2042, 2345
Bme18I	GGWCC	2 cut(s)	1729, 2241
BmgBI	CACGTC	1 cut(s)	823
BmgT120I	GGNCC	3 cut(s)	609, 1729, 2241
BmiI	GGNNCC	8 cut(s)	241, 655, 1059, 1453, 2073, 2143, 2242, 2520
BmrFI	CCNGG	8 cut(s)	35, 72, 258, 802, 1152, 1229, 2042, 2345
BmrI	ACTGGG	2 cut(s)	1143, 2093
BmsI	GCATC	5 cut(s)	636, 817, 1154, 1180, 1576
BmtI	GCTAGC	2 cut(s)	51, 2537
BmuI	ACTGGG	2 cut(s)	1143, 2093
BpiI	GAAGAC	2 cut(s)	825, 1781
BplI	GAGNNNNNCTC	2 cut(s)	2216, 2248
BpmI	CTGGAG	2 cut(s)	1134, 1894
Bpu10I	CCTNAGC	1 cut(s)	780
Bpu14I	TTCGAA	1 cut(s)	411
BpuEI	CTTGAG	3 cut(s)	589, 1538, 2529
BpuMI	CCSGG	1 cut(s)	802
BsaBI	GATNNNNATC	1 cut(s)	2177
BsaHI	GRCGYC	1 cut(s)	1549
BsaJI	CCNNGG	5 cut(s)	283, 657, 1333, 1954, 2041
BsaWI	WCCGGW	3 cut(s)	621, 740, 1792
BsaXI	ACNNNNNCTCC	8 cut(s)	369, 399, 546, 576, 777, 807, 1146, 1176
Bsc4I	CCNNNNNNNGG	8 cut(s)	740, 747, 807, 1338, 1339, 1780, 1946, 2083
Bse1I	ACTGG	6 cut(s)	646, 982, 1138, 1462, 1888, 2088
Bse3DI	GCAATG	2 cut(s)	1731, 1785
Bse8I	GATNNNNATC	1 cut(s)	2177
BseBI	CCWGG	7 cut(s)	35, 72, 258, 1152, 1229, 2042, 2345
BseDI	CCNNGG	5 cut(s)	283, 657, 1333, 1954, 2041
BseGI	GGATG	7 cut(s)	832, 954, 1084, 1371, 1666, 1789, 1903
BseJI	GATNNNNATC	1 cut(s)	2177
BseLI	CCNNNNNNNGG	8 cut(s)	740, 747, 807, 1338, 1339, 1780, 1946, 2083
BseMI	GCAATG	2 cut(s)	1731, 1785
BseMII	CTCAG	6 cut(s)	794, 1884, 2052, 2124, 2214, 2571
BseNI	ACTGG	6 cut(s)	646, 982, 1138, 1462, 1888, 2088
BseRI	GAGGAG	3 cut(s)	161, 554, 1482
BseSI	GKGCMC	1 cut(s)	1615
BsgI	GTGCAG	3 cut(s)	194, 1981, 2424
Bsh1236I	CGCG	3 cut(s)	659, 963, 1956
BshFI	GGCC	6 cut(s)	317, 611, 1866, 2322, 2413, 2483
BshNI	GGYRCC	3 cut(s)	239, 2141, 2518
BsiHKAI	GWGCWC	3 cut(s)	1128, 1615, 1989
BsiSI	CCGG	6 cut(s)	622, 741, 801, 848, 1793, 2484
BslFI	GGGAC	4 cut(s)	50, 611, 721, 2254
BslI	CCNNNNNNNGG	8 cut(s)	740, 747, 807, 1338, 1339, 1780, 1946, 2083
BsmAI	GTCTC	4 cut(s)	229, 1556, 2032, 2560
BsmBI	CGTCTC	1 cut(s)	1556
BsmFI	GGGAC	4 cut(s)	50, 611, 721, 2254
BsnI	GGCC	6 cut(s)	317, 611, 1866, 2322, 2413, 2483
Bsp119I	TTCGAA	1 cut(s)	411
Bsp1286I	GDGCHC	3 cut(s)	1128, 1615, 1989
BspANI	GGCC	6 cut(s)	317, 611, 1866, 2322, 2413, 2483
BspCNI	CTCAG	6 cut(s)	793, 1885, 2053, 2125, 2215, 2570
BspFNI	CGCG	3 cut(s)	659, 963, 1956
BspLI	GGNNCC	8 cut(s)	241, 655, 1059, 1453, 2073, 2143, 2242, 2520
BspMAI	CTGCAG	1 cut(s)	2026
BspMI	ACCTGC	1 cut(s)	1150
BspOI	GCTAGC	2 cut(s)	51, 2537
BspPI	GGATC	7 cut(s)	197, 542, 812, 1446, 1459, 2173, 2330
BspT104I	TTCGAA	1 cut(s)	411
BspT107I	GGYRCC	3 cut(s)	239, 2141, 2518
BsrDI	GCAATG	2 cut(s)	1731, 1785
BsrI	ACTGG	6 cut(s)	646, 982, 1138, 1462, 1888, 2088
BssECI	CCNNGG	5 cut(s)	283, 657, 1333, 1954, 2041
BssNI	GRCGYC	1 cut(s)	1549
BssT1I	CCWWGG	1 cut(s)	283
Bst2UI	CCWGG	7 cut(s)	35, 72, 258, 1152, 1229, 2042, 2345
Bst4CI	ACNGT	8 cut(s)	274, 878, 928, 1092, 1417, 1610, 2149, 2276
Bst6I	CTCTTC	2 cut(s)	222, 408
BstACI	GRCGYC	1 cut(s)	1549
BstAPI	GCANNNNNTGC	2 cut(s)	1190, 2474
BstBI	TTCGAA	1 cut(s)	411
BstDEI	CTNAG	6 cut(s)	780, 1893, 2061, 2133, 2223, 2557
BstDSI	CCRYGG	3 cut(s)	657, 1333, 1954
BstEII	GGTNACC	1 cut(s)	1137
BstF5I	GGATG	7 cut(s)	832, 954, 1084, 1371, 1666, 1789, 1903
BstFNI	CGCG	3 cut(s)	659, 963, 1956
BstH2I	RGCGCY	1 cut(s)	337
BstHHI	GCGC	4 cut(s)	336, 1367, 1591, 2009
BstMAI	GTCTC	4 cut(s)	229, 1556, 2032, 2560
BstNI	CCWGG	7 cut(s)	35, 72, 258, 1152, 1229, 2042, 2345
BstNSI	RCATGY	8 cut(s)	99, 373, 847, 1040, 1208, 1274, 1648, 2007
BstPI	GGTNACC	1 cut(s)	1137
BstSCI	CCNGG	8 cut(s)	33, 70, 256, 800, 1150, 1227, 2040, 2343
BstSFI	CTRYAG	2 cut(s)	1002, 2022
BstSLI	GKGCMC	1 cut(s)	1615
BstUI	CGCG	3 cut(s)	659, 963, 1956
BstV2I	GAAGAC	2 cut(s)	825, 1781
BstX2I	RGATCY	5 cut(s)	547, 1451, 1751, 2178, 2564
BstYI	RGATCY	5 cut(s)	547, 1451, 1751, 2178, 2564
BsuRI	GGCC	6 cut(s)	317, 611, 1866, 2322, 2413, 2483
BtgI	CCRYGG	3 cut(s)	657, 1333, 1954
BtrI	CACGTC	1 cut(s)	823
BtsCI	GGATG	7 cut(s)	832, 954, 1084, 1371, 1666, 1789, 1903
BtsI	GCAGTG	2 cut(s)	1820, 2116
BtsIMutI	CAGTG	5 cut(s)	270, 1131, 1455, 1820, 2116
BveI	ACCTGC	1 cut(s)	1150
CaiI	CAGNNNCTG	1 cut(s)	476
CfoI	GCGC	4 cut(s)	336, 1367, 1591, 2009
Cfr13I	GGNCC	3 cut(s)	609, 1729, 2241
Cfr42I	CCGCGG	2 cut(s)	660, 1957
CseI	GACGC	1 cut(s)	1538
Csp6I	GTAC	6 cut(s)	352, 975, 2142, 2272, 2348, 2519
CviQI	GTAC	6 cut(s)	352, 975, 2142, 2272, 2348, 2519
DdeI	CTNAG	6 cut(s)	780, 1893, 2061, 2133, 2223, 2557
EaeI	YGGCCR	2 cut(s)	315, 1864
Eam1104I	CTCTTC	2 cut(s)	222, 408
EarI	CTCTTC	2 cut(s)	222, 408
EciI	GGCGGA	1 cut(s)	1793
Ecl136II	GAGCTC	1 cut(s)	1987
Eco130I	CCWWGG	1 cut(s)	283
Eco147I	AGGCCT	2 cut(s)	2322, 2413
Eco24I	GRGCYC	1 cut(s)	1989
Eco32I	GATATC	1 cut(s)	1974
Eco47I	GGWCC	2 cut(s)	1729, 2241
Eco47III	AGCGCT	1 cut(s)	335
Eco53kI	GAGCTC	1 cut(s)	1987
Eco57I	CTGAAG	3 cut(s)	528, 687, 1602
Eco91I	GGTNACC	1 cut(s)	1137
EcoICRI	GAGCTC	1 cut(s)	1987
EcoO65I	GGTNACC	1 cut(s)	1137
EcoRI	GAATTC	1 cut(s)	393
EcoRII	CCWGG	7 cut(s)	33, 70, 256, 1150, 1227, 2040, 2343
EcoRV	GATATC	1 cut(s)	1974
EcoT14I	CCWWGG	1 cut(s)	283
EcoT22I	ATGCAT	2 cut(s)	677, 1272
EcoT38I	GRGCYC	1 cut(s)	1989
ErhI	CCWWGG	1 cut(s)	283
Esp3I	CGTCTC	1 cut(s)	1556
FalI	AAGNNNNNCTT	2 cut(s)	2310, 2342
FaqI	GGGAC	4 cut(s)	50, 611, 721, 2254
FauI	CCCGC	2 cut(s)	75, 1949
FbaI	TGATCA	2 cut(s)	1689, 2353
FblI	GTMKAC	2 cut(s)	495, 1005
FokI	GGATG	7 cut(s)	839, 961, 1071, 1358, 1673, 1796, 1910
FriOI	GRGCYC	1 cut(s)	1989
FspI	TGCGCA	1 cut(s)	2008
GlaI	GCGC	4 cut(s)	335, 1366, 1590, 2008
GsuI	CTGGAG	2 cut(s)	1134, 1894
HaeII	RGCGCY	1 cut(s)	337
HaeIII	GGCC	6 cut(s)	317, 611, 1866, 2322, 2413, 2483
HapII	CCGG	6 cut(s)	622, 741, 801, 848, 1793, 2484
HgaI	GACGC	1 cut(s)	1538
HhaI	GCGC	4 cut(s)	336, 1367, 1591, 2009
Hin1I	GRCGYC	1 cut(s)	1549
Hin6I	GCGC	4 cut(s)	334, 1365, 1589, 2007
HinP1I	GCGC	4 cut(s)	334, 1365, 1589, 2007
HincII	GTYRAC	2 cut(s)	664, 2122
HindII	GTYRAC	2 cut(s)	664, 2122
HindIII	AAGCTT	2 cut(s)	752, 852
HinfI	GANTC	7 cut(s)	288, 347, 590, 730, 1099, 1196, 1284
HpaI	GTTAAC	2 cut(s)	664, 2122
HpaII	CCGG	6 cut(s)	622, 741, 801, 848, 1793, 2484
HphI	GGTGA	5 cut(s)	212, 542, 1472, 1570, 1981
Hpy166II	GTNNAC	8 cut(s)	496, 664, 723, 924, 1006, 1613, 1936, 2122
Hpy8I	GTNNAC	8 cut(s)	496, 664, 723, 924, 1006, 1613, 1936, 2122
Hpy99I	CGWCG	1 cut(s)	529
HpyCH4III	ACNGT	8 cut(s)	274, 878, 928, 1092, 1417, 1610, 2149, 2276
HpyCH4IV	ACGT	4 cut(s)	358, 822, 2124, 2496
HpyF3I	CTNAG	6 cut(s)	780, 1893, 2061, 2133, 2223, 2557
HpySE526I	ACGT	4 cut(s)	358, 822, 2124, 2496
Hsp92I	GRCGYC	1 cut(s)	1549
HspAI	GCGC	4 cut(s)	334, 1365, 1589, 2007
KpnI	GGTACC	2 cut(s)	2145, 2522
Ksp22I	TGATCA	2 cut(s)	1689, 2353
KspAI	GTTAAC	2 cut(s)	664, 2122
KspI	CCGCGG	2 cut(s)	660, 1957
LmnI	GCTCC	6 cut(s)	567, 624, 862, 986, 1154, 1852
LweI	GCATC	5 cut(s)	636, 817, 1154, 1180, 1576
MaeII	ACGT	4 cut(s)	358, 822, 2124, 2496
MaeIII	GTNAC	4 cut(s)	951, 1137, 1795, 1844
MflI	RGATCY	5 cut(s)	547, 1451, 1751, 2178, 2564
MhlI	GDGCHC	3 cut(s)	1128, 1615, 1989
MlsI	TGGCCA	1 cut(s)	1866
MluCI	AATT	9 cut(s)	246, 393, 419, 764, 838, 882, 1767, 1829, 1922
MluNI	TGGCCA	1 cut(s)	1866
MlyI	GAGTC	2 cut(s)	282, 341
MmeI	TCCRAC	4 cut(s)	946, 1127, 2143, 2407
Mox20I	TGGCCA	1 cut(s)	1866
Mph1103I	ATGCAT	2 cut(s)	677, 1272
MroXI	GAANNNNTTC	2 cut(s)	700, 1833
MscI	TGGCCA	1 cut(s)	1866
MseI	TTAA	9 cut(s)	483, 576, 615, 663, 770, 993, 2121, 2420, 2513
MslI	CAYNNNNRTG	2 cut(s)	1994, 2258
Msp20I	TGGCCA	1 cut(s)	1866
MspA1I	CMGCKG	2 cut(s)	659, 1956
MspI	CCGG	6 cut(s)	622, 741, 801, 848, 1793, 2484
MspR9I	CCNGG	8 cut(s)	35, 72, 258, 802, 1152, 1229, 2042, 2345
MvaI	CCWGG	7 cut(s)	35, 72, 258, 1152, 1229, 2042, 2345
MvnI	CGCG	3 cut(s)	659, 963, 1956
NciI	CCSGG	1 cut(s)	802
NheI	GCTAGC	2 cut(s)	47, 2533
NlaIV	GGNNCC	8 cut(s)	241, 655, 1059, 1453, 2073, 2143, 2242, 2520
NsbI	TGCGCA	1 cut(s)	2008
NsiI	ATGCAT	2 cut(s)	677, 1272
NspI	RCATGY	8 cut(s)	99, 373, 847, 1040, 1208, 1274, 1648, 2007
NspV	TTCGAA	1 cut(s)	411
PaeI	GCATGC	6 cut(s)	99, 847, 1040, 1208, 1274, 2007
PceI	AGGCCT	2 cut(s)	2322, 2413
PciI	ACATGT	2 cut(s)	369, 1644
PdmI	GAANNNNTTC	2 cut(s)	700, 1833
PfeI	GAWTC	5 cut(s)	590, 730, 1099, 1196, 1284
PflFI	GACNNNGTC	1 cut(s)	272
PfoI	TCCNGGA	1 cut(s)	1150
PleI	GAGTC	2 cut(s)	282, 341
PpsI	GAGTC	2 cut(s)	282, 341
PscI	ACATGT	2 cut(s)	369, 1644
Psp124BI	GAGCTC	1 cut(s)	1989
Psp6I	CCWGG	7 cut(s)	33, 70, 256, 1150, 1227, 2040, 2343
PspEI	GGTNACC	1 cut(s)	1137
PspGI	CCWGG	7 cut(s)	33, 70, 256, 1150, 1227, 2040, 2343
PspN4I	GGNNCC	8 cut(s)	241, 655, 1059, 1453, 2073, 2143, 2242, 2520
PspPI	GGNCC	3 cut(s)	609, 1729, 2241
PstI	CTGCAG	1 cut(s)	2026
PstNI	CAGNNNCTG	1 cut(s)	476
PsuI	RGATCY	5 cut(s)	547, 1451, 1751, 2178, 2564
PsyI	GACNNNGTC	1 cut(s)	272
RsaI	GTAC	6 cut(s)	353, 976, 2143, 2273, 2349, 2520
RsaNI	GTAC	6 cut(s)	352, 975, 2142, 2272, 2348, 2519
RseI	CAYNNNNRTG	2 cut(s)	1994, 2258
SacI	GAGCTC	1 cut(s)	1989
SacII	CCGCGG	2 cut(s)	660, 1957
SaqAI	TTAA	9 cut(s)	483, 576, 615, 663, 770, 993, 2121, 2420, 2513
Sau96I	GGNCC	3 cut(s)	609, 1729, 2241
SchI	GAGTC	2 cut(s)	282, 341
ScrFI	CCNGG	8 cut(s)	35, 72, 258, 802, 1152, 1229, 2042, 2345
SduI	GDGCHC	3 cut(s)	1128, 1615, 1989
SfaNI	GCATC	5 cut(s)	636, 817, 1154, 1180, 1576
SfcI	CTRYAG	2 cut(s)	1002, 2022
Sfr303I	CCGCGG	2 cut(s)	660, 1957
SfuI	TTCGAA	1 cut(s)	411
SgrBI	CCGCGG	2 cut(s)	660, 1957
SinI	GGWCC	2 cut(s)	1729, 2241
SmiMI	CAYNNNNRTG	2 cut(s)	1994, 2258
SmlI	CTYRAG	3 cut(s)	604, 1553, 2544
SmoI	CTYRAG	3 cut(s)	604, 1553, 2544
SphI	GCATGC	6 cut(s)	99, 847, 1040, 1208, 1274, 2007
Sse9I	AATT	9 cut(s)	246, 393, 419, 764, 838, 882, 1767, 1829, 1922
SseBI	AGGCCT	2 cut(s)	2322, 2413
SstI	GAGCTC	1 cut(s)	1989
StuI	AGGCCT	2 cut(s)	2322, 2413
StyD4I	CCNGG	8 cut(s)	33, 70, 256, 800, 1150, 1227, 2040, 2343
StyI	CCWWGG	1 cut(s)	283
TaaI	ACNGT	8 cut(s)	274, 878, 928, 1092, 1417, 1610, 2149, 2276
TaiI	ACGT	4 cut(s)	361, 825, 2127, 2499
TaqI	TCGA	3 cut(s)	411, 519, 2175
TasI	AATT	9 cut(s)	246, 393, 419, 764, 838, 882, 1767, 1829, 1922
TauI	GCSGC	5 cut(s)	245, 320, 963, 1322, 1700
TfiI	GAWTC	5 cut(s)	590, 730, 1099, 1196, 1284
Tru1I	TTAA	9 cut(s)	483, 576, 615, 663, 770, 993, 2121, 2420, 2513
Tru9I	TTAA	9 cut(s)	483, 576, 615, 663, 770, 993, 2121, 2420, 2513
TscAI	CASTG	5 cut(s)	277, 1138, 1462, 1827, 2116
TspDTI	ATGAA	7 cut(s)	17, 848, 1073, 1440, 1842, 1914, 2490
TspGWI	ACGGA	3 cut(s)	52, 1763, 2405
TspRI	CASTG	5 cut(s)	277, 1138, 1462, 1827, 2116
Tth111I	GACNNNGTC	1 cut(s)	272
VneI	GTGCAC	1 cut(s)	1611
VpaK11BI	GGWCC	2 cut(s)	1729, 2241
XapI	RAATTY	5 cut(s)	393, 764, 882, 1767, 1829
XbaI	TCTAGA	1 cut(s)	886
XceI	RCATGY	8 cut(s)	99, 373, 847, 1040, 1208, 1274, 1648, 2007
XcmI	CCANNNNNNNNNTGG	1 cut(s)	138
XmiI	GTMKAC	2 cut(s)	495, 1005
XmnI	GAANNNNTTC	2 cut(s)	700, 1833
Zsp2I	ATGCAT	2 cut(s)	677, 1272

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.