MD13G1177400.v1.1

Pyruvate phosphate dikinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004385
Physical Location & Seq
Reverse (-)
14722418 .. 14728213
5796 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1177400.v1.1.491

Sequence Viewer

Length: 2658 bp
ATGAGCCAGCACAAAGTCAATCAAACTCCGTTCTGTCCCCAGTTGCAGATCAAGCGAGTATTTACTTTCGGAAAAGGAAAGAGTGAAGGCAACAAGGGAATGAAGTCATTGTTGGGAGGAAAAGGTGCAAACCTGGCAGAAATGGCTAGCATTGGGCTATCCGTCCCACCTGGACTTACTATATCAGCAGAAGCATGCAGAGATTATCAGCTCAATGGCAAAGACTTACCACAAGGTTTGTGGGAGGAGATATTAGAGGGCTTGGAGAGTGTGGAAAAGGACATTGGTGCTATTCTTGGTGATTCTTCCAAGCCTCTTCTCGTCTCTGTACGCTCTGGTGCCGCGGTTTCCATGCCTGGCATGATGGACACTGTCCTCAACCTTGGACTCAATGACAAGGTGGTTGCTGGTTTGTCCGCAAAAAGCGGAGAGCGCTTTGCTTATGACTCGTACAGACGTTTCCTAGACATGTTTGGAGATGTTGTTATGGGAATTCCACACTCATCGTTTGAGGAGAAATTGGAAAAACTAAAAGGTAAAAAAGGAGTTAAGCTTGATACAAAGCTAACAGCTTCTGATCTTAAAGAGCTGGTAGAACAATACAAAAATGTTTATCTCGAAGCAACGGGAGAAAAGTTCCCTTCAGATCCAAAACAACAGCTTCTCTTGGCAGTTAAAGCAGTGTTTGATTCTTGGGACAGCCAAAGGGCCATTAAATACCGGAGCATCAATCAGATAACTGGTTTGAAGGGAACCGCGGTTAACATTCAATGCATGGTTTTCGGGAACATGGGAAATACTTCAGGGACTGGTGTTCTGTTCACTAGGAATCCAAGCACCGGTGAAAGGAAGCTTTATGGCGAGTTCCTAGTCAATGCTCAGGGAGAAGATGTAGTTGCCGGGATCAGGACACCAGAAGACGTGGAGGCTATGAAAAATTGCATGCCCGAAGCTTACAAGGAGCTTGTGGAGAACTGTGAAATTCTGGAAAAACATTACAAAGATATGATGGACATTGAGTTCACAGTCCAAGAAAATAGGTTGTGGATGTTGCAATGCCGTGTTGGAAAGCGTACTGGTAAAGGAGCGATTAAGATAGCTGTAGACATGGCTAACGAAGGGCTTGTGGATAAGCACACTGCAATCAAGATGGTGGAACCACAGCACCTTGACCAACTTCTTCACCCACAGTTTGAAAATCCAACTGCTTACAAAGACAAAGTGATCGCCACTGGGTTACCTGCATCTCCAGGAGCAGCAGTAGGACAAGTCGTGTTCACTGCAGAAGATGCTGAATCATGGCATGCACAAGGAAAGAAAGTCATCCTGGTAAGGACAGAAACTAGCCCCGAAGATGTTGGGGGTATGCATGCTGCTGCCGGAATCTTGACAGCTCGAGGTGGAATGACATCTCATGCAGCTGTTGTAGCGCGTGGGTGGGGTAAATGTTGTGTTTCTGGCTGCGCTGACATCCGAGTAAATGACAGTGAGAAGCTGGTTTTGATCGGGAAAACGGTGGTCAATGAAGGAGAATGGCTTTCACTCAATGGGTCCACTGGTGAAGTCATATTGGGAAAACAGCCACTTGCTCCTCCGGCTTTGAGTGGCGATTTGGAAACCTTTATGTCTTGGGCTGATAAAGTCAGGCGTCTCAAGGTGATGGCAAATGCTGACACGCCTGAAGATGCAATAACAGCAAGAAATAACGGTGCACAAGGGATTGGACTTTGCAGGACAGAACACATGTTCTTTGCTTCGGATGATAGAATAAAGGCGGTAAGAAAAATGATCATGGCAGCTACACTTGAACAGAGGAAGGCAGCATTGGACCTCTTACTACCGTATCAGAGATCCGACTTTGAAGGAATTTTCCGTGCAATGGATGGTCTTCCAGTAACAATCCGCCTGTTAGACCCTCCACTTCATGAATTTCTTCCAGAAGGTAACTTGGAACAGATTGTCGGCCAACTAACTACACAGACTGGCATGACCGAGGACGAAGTTTTCTCAAGAGTTGAGAAATTATCAGAAGTAAACCCCATGCTTGGTTTCCGCGGCTGCAGGCTAGGGATATCGTACCCAGAGCTCACGGAAATGCAGGCGCGTGCAATCTTTCAAGCTGCTGTCACAATGAGCAACCAGGGTGTCAAAGTTTTCCCCGAGATAATGGTTCCCCTTGAACTGGGACATCAAGTGAGTCTCATTCGCAGTGTCGCAGTGAAAGTGTTCTCTGAGATGGGTACCACCGTGACCTATAAAGTTGGAACTATGATCGAGATCCCTAGAGCTGCTCTGGTTGCAGATGAGATTGCAAAGGAAGCTGAGTTCTTCTCCTTCGGGACCAATGATCTCACACAAATGACATTTGGGTACAGTAGAGATGATGTGGGCAAGTTTCTACCTATTTACCTCTCCAAAGGCCTTCTTCAAAACGATCCCTTCCAGGTACTTGATCAAAGAGGTGTCGGGCAGCTCATTAAGATGGCCACAGAAGAGGGTCGCGCAGCTAGGCCTAGCTTAAAGGTTGGAGTATGTGGAGAGCATGGTGGAGACCCTTCTTCTGTTGCATTTTTCGCAGAGGCCGGACTAGACTATGTTTCGTGTTCTCCATTCAGGGTACCTATTGCTAGGCTAGCAGCAGCTCAAGTGGCTCTCTGA

Protein Analysis

886

Amino Acids

96.31

Weight (kDa)

5.82

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPDK_N PF01326 36 - 91 4.3e-07 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N PF01326 75 - 311 2.8e-24 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N PF01326 318 - 372 5.9e-10 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PEP-utilizers PF00391 439 - 520 5.1e-23 PEP-utilising enzyme, mobile domain
PEP-utilizers_C PF02896 535 - 883 5.3e-89 PEP-utilising enzyme, PEP-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1249
Acc65I GGTACC 2 cut(s) 2240, 2617
AccB1I GGYRCC 3 cut(s) 338, 2240, 2617
AccI GTMKAC 1 cut(s) 1104
AccII CGCG 6 cut(s) 344, 758, 1432, 2055, 2104, 2502
AclWI GGATC 5 cut(s) 641, 911, 1845, 2272, 2429
AcoI YGGCCR 2 cut(s) 1963, 2484
AcsI RAATTY 4 cut(s) 492, 981, 1866, 1928
AcuI CTGAAG 3 cut(s) 627, 786, 1701
AcyI GRCGYC 1 cut(s) 1648
AfaI GTAC 8 cut(s) 330, 452, 1075, 2078, 2242, 2372, 2448, 2619
AfeI AGCGCT 1 cut(s) 434
AfiI CCNNNNNNNGG 6 cut(s) 839, 846, 906, 1879, 2045, 2182
AflIII ACRYGT 2 cut(s) 468, 1743
AgeI ACCGGT 1 cut(s) 839
AgsI TTSAA 8 cut(s) 748, 770, 1196, 1808, 1862, 2117, 2180, 2429
AjiI CACGTC 1 cut(s) 922
AjnI CCWGG 7 cut(s) 132, 169, 355, 1249, 1326, 2139, 2442
AjuI GAANNNNNNNTTGG 6 cut(s) 95, 127, 267, 299, 1808, 1840
AloI GAACNNNNNNTCC 4 cut(s) 1004, 1036, 2309, 2341
Alw21I GWGCWC 2 cut(s) 1714, 2088
Alw26I GTCTC 4 cut(s) 328, 1655, 2204, 2544
Alw44I GTGCAC 1 cut(s) 1710
AlwI GGATC 5 cut(s) 641, 911, 1845, 2272, 2429
AlwNI CAGNNNCTG 2 cut(s) 575, 809
Ama87I CYCGRG 2 cut(s) 1395, 2159
Aor51HI AGCGCT 1 cut(s) 434
AoxI GGCC 6 cut(s) 708, 1963, 2419, 2484, 2510, 2580
ApaLI GTGCAC 1 cut(s) 1710
ApoI RAATTY 4 cut(s) 492, 981, 1866, 1928
AsiGI ACCGGT 1 cut(s) 839
Asp700I GAANNNNTTC 3 cut(s) 799, 1932, 2225
Asp718I GGTACC 2 cut(s) 2240, 2617
AspLEI GCGC 5 cut(s) 435, 1432, 1466, 2104, 2504
AspS9I GGNCC 4 cut(s) 708, 1551, 1828, 2340
AsuC2I CCSGG 1 cut(s) 901
AsuHPI GGTGA 5 cut(s) 311, 854, 1175, 1571, 1669
AsuNHI GCTAGC 2 cut(s) 146, 2632
AvaI CYCGRG 2 cut(s) 1395, 2159
AvaII GGWCC 3 cut(s) 1551, 1828, 2340
BaeGI GKGCMC 1 cut(s) 1714
BaeI ACNNNNGTAYC 4 cut(s) 1826, 1859, 2232, 2265
BalI TGGCCA 1 cut(s) 2486
BanI GGYRCC 3 cut(s) 338, 2240, 2617
BanII GRGCYC 1 cut(s) 2088
BbsI GAAGAC 2 cut(s) 924, 1880
Bbv12I GWGCWC 2 cut(s) 1714, 2088
BccI CCATC 7 cut(s) 358, 1003, 1144, 1654, 1877, 2230, 2476
BceAI ACGGC 1 cut(s) 1044
BcgI CGANNNNNNTGC 2 cut(s) 763, 797
BciT130I CCWGG 7 cut(s) 134, 171, 357, 1251, 1328, 2141, 2444
BclI TGATCA 2 cut(s) 1788, 2452
BcnI CCSGG 1 cut(s) 901
BcoDI GTCTC 4 cut(s) 328, 1655, 2204, 2544
BfmI CTRYAG 3 cut(s) 1101, 1281, 2059
BfoI RGCGCY 1 cut(s) 436
BfuAI ACCTGC 1 cut(s) 1249
Bme1390I CCNGG 8 cut(s) 134, 171, 357, 901, 1251, 1328, 2141, 2444
Bme18I GGWCC 3 cut(s) 1551, 1828, 2340
BmeT110I CYCGRG 2 cut(s) 1395, 2159
BmgBI CACGTC 1 cut(s) 922
BmgT120I GGNCC 4 cut(s) 708, 1551, 1828, 2340
BmiI GGNNCC 8 cut(s) 340, 754, 1158, 1552, 2172, 2242, 2341, 2619
BmrFI CCNGG 8 cut(s) 134, 171, 357, 901, 1251, 1328, 2141, 2444
BmrI ACTGGG 3 cut(s) 34, 1242, 2192
BmsI GCATC 4 cut(s) 735, 1253, 1279, 1675
BmtI GCTAGC 2 cut(s) 150, 2636
BmuI ACTGGG 3 cut(s) 34, 1242, 2192
BpiI GAAGAC 2 cut(s) 924, 1880
BplI GAGNNNNNCTC 2 cut(s) 2315, 2347
BpmI CTGGAG 1 cut(s) 1233
Bpu10I CCTNAGC 1 cut(s) 879
BpuEI CTTGAG 3 cut(s) 1637, 1993, 2628
BpuMI CCSGG 1 cut(s) 901
BsaBI GATNNNNATC 1 cut(s) 2276
BsaHI GRCGYC 1 cut(s) 1648
BsaI GGTCTC 1 cut(s) 2544
BsaJI CCNNGG 6 cut(s) 342, 382, 756, 1992, 2053, 2140
BsaWI WCCGGW 2 cut(s) 720, 839
BsaXI ACNNNNNCTCC 6 cut(s) 468, 498, 876, 906, 1245, 1275
Bsc4I CCNNNNNNNGG 6 cut(s) 839, 846, 906, 1879, 2045, 2182
Bse118I RCCGGY 1 cut(s) 839
Bse1I ACTGG 9 cut(s) 40, 745, 814, 1081, 1237, 1561, 1892, 1987, 2187
Bse3DI GCAATG 2 cut(s) 1061, 1884
Bse8I GATNNNNATC 1 cut(s) 2276
BseBI CCWGG 7 cut(s) 134, 171, 357, 1251, 1328, 2141, 2444
BseDI CCNNGG 6 cut(s) 342, 382, 756, 1992, 2053, 2140
BseGI GGATG 5 cut(s) 1053, 1323, 1470, 1765, 1888
BseJI GATNNNNATC 1 cut(s) 2276
BseLI CCNNNNNNNGG 6 cut(s) 839, 846, 906, 1879, 2045, 2182
BseMI GCAATG 2 cut(s) 1061, 1884
BseMII CTCAG 3 cut(s) 893, 2223, 2313
BseNI ACTGG 9 cut(s) 40, 745, 814, 1081, 1237, 1561, 1892, 1987, 2187
BseRI GAGGAG 3 cut(s) 260, 527, 1581
BseSI GKGCMC 1 cut(s) 1714
Bsh1236I CGCG 6 cut(s) 344, 758, 1432, 2055, 2104, 2502
BshFI GGCC 6 cut(s) 710, 1965, 2421, 2486, 2512, 2582
BshNI GGYRCC 3 cut(s) 338, 2240, 2617
BshTI ACCGGT 1 cut(s) 839
BsiHKAI GWGCWC 2 cut(s) 1714, 2088
BsiHKCI CYCGRG 2 cut(s) 1395, 2159
BsiSI CCGG 6 cut(s) 721, 840, 900, 1380, 1595, 2583
BslFI GGGAC 6 cut(s) 21, 149, 710, 820, 2199, 2353
BslI CCNNNNNNNGG 6 cut(s) 839, 846, 906, 1879, 2045, 2182
BsmAI GTCTC 4 cut(s) 328, 1655, 2204, 2544
BsmBI CGTCTC 2 cut(s) 328, 1655
BsmFI GGGAC 6 cut(s) 21, 149, 710, 820, 2199, 2353
BsnI GGCC 6 cut(s) 710, 1965, 2421, 2486, 2512, 2582
Bso31I GGTCTC 1 cut(s) 2544
BsoBI CYCGRG 2 cut(s) 1395, 2159
Bsp1286I GDGCHC 2 cut(s) 1714, 2088
BspANI GGCC 6 cut(s) 710, 1965, 2421, 2486, 2512, 2582
BspCNI CTCAG 3 cut(s) 892, 2224, 2314
BspFNI CGCG 6 cut(s) 344, 758, 1432, 2055, 2104, 2502
BspHI TCATGA 1 cut(s) 1924
BspLI GGNNCC 8 cut(s) 340, 754, 1158, 1552, 2172, 2242, 2341, 2619
BspMAI CTGCAG 2 cut(s) 1285, 2063
BspMI ACCTGC 1 cut(s) 1249
BspOI GCTAGC 2 cut(s) 150, 2636
BspPI GGATC 5 cut(s) 641, 911, 1845, 2272, 2429
BspT107I GGYRCC 3 cut(s) 338, 2240, 2617
BspTNI GGTCTC 1 cut(s) 2544
BsrDI GCAATG 2 cut(s) 1061, 1884
BsrFI RCCGGY 1 cut(s) 839
BsrI ACTGG 9 cut(s) 40, 745, 814, 1081, 1237, 1561, 1892, 1987, 2187
BssAI RCCGGY 1 cut(s) 839
BssECI CCNNGG 6 cut(s) 342, 382, 756, 1992, 2053, 2140
BssNI GRCGYC 1 cut(s) 1648
BssT1I CCWWGG 1 cut(s) 382
Bst2UI CCWGG 7 cut(s) 134, 171, 357, 1251, 1328, 2141, 2444
Bst6I CTCTTC 2 cut(s) 321, 2487
BstACI GRCGYC 1 cut(s) 1648
BstAPI GCANNNNNTGC 1 cut(s) 1289
BstDEI CTNAG 3 cut(s) 879, 2232, 2322
BstDSI CCRYGG 3 cut(s) 342, 756, 2053
BstEII GGTNACC 1 cut(s) 1236
BstF5I GGATG 5 cut(s) 1053, 1323, 1470, 1765, 1888
BstFNI CGCG 6 cut(s) 344, 758, 1432, 2055, 2104, 2502
BstH2I RGCGCY 1 cut(s) 436
BstHHI GCGC 5 cut(s) 435, 1432, 1466, 2104, 2504
BstMAI GTCTC 4 cut(s) 328, 1655, 2204, 2544
BstNI CCWGG 7 cut(s) 134, 171, 357, 1251, 1328, 2141, 2444
BstNSI RCATGY 6 cut(s) 198, 472, 946, 1307, 1373, 1747
BstPI GGTNACC 1 cut(s) 1236
BstSCI CCNGG 8 cut(s) 132, 169, 355, 899, 1249, 1326, 2139, 2442
BstSFI CTRYAG 3 cut(s) 1101, 1281, 2059
BstSLI GKGCMC 1 cut(s) 1714
BstUI CGCG 6 cut(s) 344, 758, 1432, 2055, 2104, 2502
BstV2I GAAGAC 2 cut(s) 924, 1880
BstX2I RGATCY 3 cut(s) 646, 1850, 2277
BstYI RGATCY 3 cut(s) 646, 1850, 2277
BsuRI GGCC 6 cut(s) 710, 1965, 2421, 2486, 2512, 2582
BtgI CCRYGG 3 cut(s) 342, 756, 2053
BtrI CACGTC 1 cut(s) 922
BtsCI GGATG 5 cut(s) 1053, 1323, 1470, 1765, 1888
BtsI GCAGTG 5 cut(s) 687, 1137, 1278, 2215, 2223
BtsIMutI CAGTG 9 cut(s) 369, 687, 1137, 1230, 1278, 1492, 1554, 2215, 2223
BveI ACCTGC 1 cut(s) 1249
CaiI CAGNNNCTG 2 cut(s) 575, 809
CciI TCATGA 1 cut(s) 1924
CfoI GCGC 5 cut(s) 435, 1432, 1466, 2104, 2504
Cfr10I RCCGGY 1 cut(s) 839
Cfr13I GGNCC 4 cut(s) 708, 1551, 1828, 2340
Cfr42I CCGCGG 3 cut(s) 345, 759, 2056
CseI GACGC 1 cut(s) 1637
Csp6I GTAC 8 cut(s) 329, 451, 1074, 2077, 2241, 2371, 2447, 2618
CspAI ACCGGT 1 cut(s) 839
CspCI CAANNNNNGTGG 4 cut(s) 219, 221, 254, 256
CviQI GTAC 8 cut(s) 329, 451, 1074, 2077, 2241, 2371, 2447, 2618
DdeI CTNAG 3 cut(s) 879, 2232, 2322
EaeI YGGCCR 2 cut(s) 1963, 2484
Eam1104I CTCTTC 2 cut(s) 321, 2487
EarI CTCTTC 2 cut(s) 321, 2487
EciI GGCGGA 1 cut(s) 1892
Ecl136II GAGCTC 1 cut(s) 2086
Eco130I CCWWGG 1 cut(s) 382
Eco147I AGGCCT 2 cut(s) 2421, 2512
Eco24I GRGCYC 1 cut(s) 2088
Eco31I GGTCTC 1 cut(s) 2544
Eco32I GATATC 1 cut(s) 2073
Eco47I GGWCC 3 cut(s) 1551, 1828, 2340
Eco47III AGCGCT 1 cut(s) 434
Eco53kI GAGCTC 1 cut(s) 2086
Eco57I CTGAAG 3 cut(s) 627, 786, 1701
Eco88I CYCGRG 2 cut(s) 1395, 2159
Eco91I GGTNACC 1 cut(s) 1236
EcoICRI GAGCTC 1 cut(s) 2086
EcoO65I GGTNACC 1 cut(s) 1236
EcoRI GAATTC 1 cut(s) 492
EcoRII CCWGG 7 cut(s) 132, 169, 355, 1249, 1326, 2139, 2442
EcoRV GATATC 1 cut(s) 2073
EcoT14I CCWWGG 1 cut(s) 382
EcoT22I ATGCAT 2 cut(s) 776, 1371
EcoT38I GRGCYC 1 cut(s) 2088
ErhI CCWWGG 1 cut(s) 382
Esp3I CGTCTC 2 cut(s) 328, 1655
FalI AAGNNNNNCTT 2 cut(s) 2409, 2441
FaqI GGGAC 6 cut(s) 21, 149, 710, 820, 2199, 2353
FbaI TGATCA 2 cut(s) 1788, 2452
FblI GTMKAC 1 cut(s) 1104
FokI GGATG 5 cut(s) 1060, 1310, 1457, 1772, 1895
FriOI GRGCYC 1 cut(s) 2088
GlaI GCGC 5 cut(s) 434, 1431, 1465, 2103, 2503
GsuI CTGGAG 1 cut(s) 1233
HaeII RGCGCY 1 cut(s) 436
HaeIII GGCC 6 cut(s) 710, 1965, 2421, 2486, 2512, 2582
HapII CCGG 6 cut(s) 721, 840, 900, 1380, 1595, 2583
HgaI GACGC 1 cut(s) 1637
HhaI GCGC 5 cut(s) 435, 1432, 1466, 2104, 2504
Hin1I GRCGYC 1 cut(s) 1648
Hin6I GCGC 5 cut(s) 433, 1430, 1464, 2102, 2502
HinP1I GCGC 5 cut(s) 433, 1430, 1464, 2102, 2502
HincII GTYRAC 1 cut(s) 763
HindII GTYRAC 1 cut(s) 763
HindIII AAGCTT 3 cut(s) 551, 851, 951
HinfI GANTC 8 cut(s) 302, 387, 446, 689, 829, 1295, 1383, 2197
HpaI GTTAAC 1 cut(s) 763
HpaII CCGG 6 cut(s) 721, 840, 900, 1380, 1595, 2583
HphI GGTGA 5 cut(s) 311, 854, 1175, 1571, 1669
Hpy166II GTNNAC 8 cut(s) 763, 822, 1023, 1105, 1278, 1554, 1712, 2035
Hpy8I GTNNAC 8 cut(s) 763, 822, 1023, 1105, 1278, 1554, 1712, 2035
HpyCH4IV ACGT 2 cut(s) 457, 921
HpyF3I CTNAG 3 cut(s) 879, 2232, 2322
HpySE526I ACGT 2 cut(s) 457, 921
Hsp92I GRCGYC 1 cut(s) 1648
HspAI GCGC 5 cut(s) 433, 1430, 1464, 2102, 2502
KpnI GGTACC 2 cut(s) 2244, 2621
Ksp22I TGATCA 2 cut(s) 1788, 2452
KspAI GTTAAC 1 cut(s) 763
KspI CCGCGG 3 cut(s) 345, 759, 2056
LmnI GCTCC 5 cut(s) 723, 961, 1085, 1253, 1594
LweI GCATC 4 cut(s) 735, 1253, 1279, 1675
MaeII ACGT 2 cut(s) 457, 921
MaeIII GTNAC 5 cut(s) 1236, 1894, 1943, 2125, 2248
MflI RGATCY 3 cut(s) 646, 1850, 2277
MhlI GDGCHC 2 cut(s) 1714, 2088
MlsI TGGCCA 1 cut(s) 2486
MluCI AATT 7 cut(s) 492, 518, 937, 981, 1866, 1928, 2021
MluNI TGGCCA 1 cut(s) 2486
MlyI GAGTC 3 cut(s) 381, 440, 2206
MmeI TCCRAC 5 cut(s) 1045, 1226, 1878, 2242, 2506
Mox20I TGGCCA 1 cut(s) 2486
Mph1103I ATGCAT 2 cut(s) 776, 1371
MroXI GAANNNNTTC 3 cut(s) 799, 1932, 2225
MscI TGGCCA 1 cut(s) 2486
MseI TTAA 8 cut(s) 549, 582, 675, 714, 762, 1092, 2478, 2519
MslI CAYNNNNRTG 3 cut(s) 2093, 2357, 2480
Msp20I TGGCCA 1 cut(s) 2486
MspA1I CMGCKG 4 cut(s) 344, 758, 1421, 2055
MspI CCGG 6 cut(s) 721, 840, 900, 1380, 1595, 2583
MspR9I CCNGG 8 cut(s) 134, 171, 357, 901, 1251, 1328, 2141, 2444
MvaI CCWGG 7 cut(s) 134, 171, 357, 1251, 1328, 2141, 2444
MvnI CGCG 6 cut(s) 344, 758, 1432, 2055, 2104, 2502
NciI CCSGG 1 cut(s) 901
NheI GCTAGC 2 cut(s) 146, 2632
NlaIV GGNNCC 8 cut(s) 340, 754, 1158, 1552, 2172, 2242, 2341, 2619
NmuCI GTSAC 2 cut(s) 2125, 2248
NsiI ATGCAT 2 cut(s) 776, 1371
NspI RCATGY 6 cut(s) 198, 472, 946, 1307, 1373, 1747
PaeI GCATGC 4 cut(s) 198, 946, 1307, 1373
PaeR7I CTCGAG 1 cut(s) 1395
PagI TCATGA 1 cut(s) 1924
PceI AGGCCT 2 cut(s) 2421, 2512
PciI ACATGT 2 cut(s) 468, 1743
PdmI GAANNNNTTC 3 cut(s) 799, 1932, 2225
PfeI GAWTC 5 cut(s) 302, 689, 829, 1295, 1383
PflFI GACNNNGTC 1 cut(s) 371
PfoI TCCNGGA 1 cut(s) 1249
PinAI ACCGGT 1 cut(s) 839
PleI GAGTC 3 cut(s) 381, 440, 2205
PpsI GAGTC 3 cut(s) 381, 440, 2205
PscI ACATGT 2 cut(s) 468, 1743
Psp124BI GAGCTC 1 cut(s) 2088
Psp6I CCWGG 7 cut(s) 132, 169, 355, 1249, 1326, 2139, 2442
PspEI GGTNACC 1 cut(s) 1236
PspGI CCWGG 7 cut(s) 132, 169, 355, 1249, 1326, 2139, 2442
PspN4I GGNNCC 8 cut(s) 340, 754, 1158, 1552, 2172, 2242, 2341, 2619
PspPI GGNCC 4 cut(s) 708, 1551, 1828, 2340
PspXI VCTCGAGB 1 cut(s) 1395
PstI CTGCAG 2 cut(s) 1285, 2063
PstNI CAGNNNCTG 2 cut(s) 575, 809
PsuI RGATCY 3 cut(s) 646, 1850, 2277
PsyI GACNNNGTC 1 cut(s) 371
PvuII CAGCTG 1 cut(s) 1421
RsaI GTAC 8 cut(s) 330, 452, 1075, 2078, 2242, 2372, 2448, 2619
RsaNI GTAC 8 cut(s) 329, 451, 1074, 2077, 2241, 2371, 2447, 2618
RseI CAYNNNNRTG 3 cut(s) 2093, 2357, 2480
SacI GAGCTC 1 cut(s) 2088
SacII CCGCGG 3 cut(s) 345, 759, 2056
SaqAI TTAA 8 cut(s) 549, 582, 675, 714, 762, 1092, 2478, 2519
Sau96I GGNCC 4 cut(s) 708, 1551, 1828, 2340
SchI GAGTC 3 cut(s) 381, 440, 2206
ScrFI CCNGG 8 cut(s) 134, 171, 357, 901, 1251, 1328, 2141, 2444
SduI GDGCHC 2 cut(s) 1714, 2088
SfaNI GCATC 4 cut(s) 735, 1253, 1279, 1675
SfcI CTRYAG 3 cut(s) 1101, 1281, 2059
Sfr274I CTCGAG 1 cut(s) 1395
Sfr303I CCGCGG 3 cut(s) 345, 759, 2056
SgrAI CRCCGGYG 1 cut(s) 839
SgrBI CCGCGG 3 cut(s) 345, 759, 2056
SinI GGWCC 3 cut(s) 1551, 1828, 2340
SlaI CTCGAG 1 cut(s) 1395
SmiMI CAYNNNNRTG 3 cut(s) 2093, 2357, 2480
SmlI CTYRAG 4 cut(s) 1395, 1652, 2008, 2643
SmoI CTYRAG 4 cut(s) 1395, 1652, 2008, 2643
SphI GCATGC 4 cut(s) 198, 946, 1307, 1373
Sse9I AATT 7 cut(s) 492, 518, 937, 981, 1866, 1928, 2021
SseBI AGGCCT 2 cut(s) 2421, 2512
SstI GAGCTC 1 cut(s) 2088
StuI AGGCCT 2 cut(s) 2421, 2512
StyD4I CCNGG 8 cut(s) 132, 169, 355, 899, 1249, 1326, 2139, 2442
StyI CCWWGG 1 cut(s) 382
TaiI ACGT 2 cut(s) 460, 924
TaqI TCGA 3 cut(s) 618, 1396, 2274
TaqII GACCGA 1 cut(s) 2006
TasI AATT 7 cut(s) 492, 518, 937, 981, 1866, 1928, 2021
TauI GCSGC 2 cut(s) 344, 2058
TfiI GAWTC 5 cut(s) 302, 689, 829, 1295, 1383
Tru1I TTAA 8 cut(s) 549, 582, 675, 714, 762, 1092, 2478, 2519
Tru9I TTAA 8 cut(s) 549, 582, 675, 714, 762, 1092, 2478, 2519
TscAI CASTG 9 cut(s) 376, 687, 1144, 1237, 1285, 1492, 1561, 2215, 2223
TseFI GTSAC 2 cut(s) 2125, 2248
Tsp45I GTSAC 2 cut(s) 2125, 2248
TspDTI ATGAA 5 cut(s) 116, 947, 1539, 1913, 1941
TspGWI ACGGA 4 cut(s) 18, 151, 1862, 2105
TspRI CASTG 9 cut(s) 376, 687, 1144, 1237, 1285, 1492, 1561, 2215, 2223
Tth111I GACNNNGTC 1 cut(s) 371
VneI GTGCAC 1 cut(s) 1710
VpaK11BI GGWCC 3 cut(s) 1551, 1828, 2340
XapI RAATTY 4 cut(s) 492, 981, 1866, 1928
XceI RCATGY 6 cut(s) 198, 472, 946, 1307, 1373, 1747
XcmI CCANNNNNNNNNTGG 1 cut(s) 237
XhoI CTCGAG 1 cut(s) 1395
XmiI GTMKAC 1 cut(s) 1104
XmnI GAANNNNTTC 3 cut(s) 799, 1932, 2225
Zsp2I ATGCAT 2 cut(s) 776, 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.