MD13G1177500.v1.1

Pyruvate phosphate dikinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004385
Physical Location & Seq
Reverse (-)
14738201 .. 14744622
6422 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1177500.v1.1.491

Sequence Viewer

Length: 2973 bp
ATGCTCATAAGGACACCACCTGAGGTTTATAGGCAGAGATTGTTGAAGGGAAAATATGTGGATCAATTTGATCTTGTTAGAGAAAACCCTTCCTTCCATGGCCTCAACTCGTCGGGTCGAGTTGGCCTCAAGCGCTGTCACCGGCAGCAGATGCAAATTGTAAACGGGATCATGAACCCAAACCCGAAGAAGAACGAGCCAGGACACATCGAAGCAAAAGCTGTTGTGTCCCCAGCTGCAGATCAGACTGCACCAACAACCAGAAAGCGAGTATTTACATTTGGAAAAGGAAAGAGTGAAGGCAACAGGGCAATGAAGTCATTGTTGGGAGGGAAAGGTGCAAACCTGGCAGAAATGGCTAGCATTGGACTATACGTCCCACCTGGACTTACTATTTCAACAGAAGCATGCCAAGAGTATCAGCTGAATGGCAAAGATTTACCGCAAGGTTTGTGGGAGGAGATACTAGAGGGCTTGGAGAGTGTGCAGAAGGACATGGGTGCTATTCTCGGTGACCCTTCCAAGCCTCTCCTCCTATCCGTTCGCTCTGGTGCTGCGATTTCCATGCCTGGCATGATGGACACTGTCCTCAACCTTGGACTCAATGACAATGTGGTTGCTGGTTTGGCCGCGAAAAGTGGAGAGCGCTTTGCTTATGACTCGTACAGGCGTTTTCTAGACATGTTTGGAAATGTTGTTATGGGAATTCCACACTCATCATTTGAGGAGCAGTTGGAAAAACTAAAAGGTACAAAAGGAGTTGAGCTTGACACAGAGCTAACAGCCTCTGATCTCAAAGAGCTGGTAGAACAATACAAAAATGTCTATCTCGAAACCACCGGTGAAAAGTTCCCTTCAGATCCAAAACAACAGCTTCTCTTGGCTGTTAAAGCAGTTTTTGATTCTTGGGACAGCCCAAGAGCCAATAAATATCGGAGCATCAATCAGATAACGGGTTTGAAGGGAACCGCGGTTAATATTCAATGCATGGTTTTCGGGAACATGGGAAATACTTCTGGCACAGGTGTTCTGTTCACTAGGAATCCAAGCACCGGTGAAAGGAAGCTTTATGGAGAATTTTTAATCAGTGCTCAGGGAGAAGATGTAGTTGCCGGCATTAGGACGCCAGAAGACTTGGATACTATGAAAAATTGTATGCCAGAAGCTTACAAGGAGCTTGTAGAGAACTGTGAAATTCTAGAAAAACATTACAAAGATATGATGGATATCGAGTTCACAGTCCAAGAAAATAGGTTGTGGATGTTACAATGTCGCAGTGGAAAGCGTACTGGTAAAGGTGCAGTGAAGATAGCTGTAGACATGGTGAACGAAGGGCTTGTGGATAAGCGCACTGCAATCAAGATGGTTGAACCACAGCACCTTGACCAACTTCTTCACCCACAGTTTGAGAATCCAACTGCTTACAAAGACAAAGTGATCGCCACTGGATTGCCTGCATCTCCAGGAGCAGCTGTAGGGACAGTTGTGTTCAGTGCTGAGGATGCCGAAACATGGCATGCACAAGGAAAGAGTGTCATCTTGGTAAGGACTGAAACTAGCCCCGAAGATGTTGGGGGTATGCATGCAGCTGCAGGGATCTTGACAGCTAGAGGAGGCATGACATCTCATGCAGCTGTTGTAGCCCGTGGGTGGGGCAAATGTTGTGTTTCTGGCTGCGCTGATATCCGAGTAAATGACGCTGAGAAGCTGGTTGTGATCGGGGATACAGTGGTCGAGGAAGGAGAATGGCTCTCACTCAATGGTTCCACTGGGGAAGTCATATTAGGAAAAGAGCCCCTTTCTCCTCCGGCTTTAAGTGGAGATTTGGAAACCTTCATGTCTTGGGCTGATAAAGTCCGGCGTCTCAAGGTTATGGCAAACGCGGACACACCTGAAGATGCGCTAACAGCAAGAAATAATGGTGCCGAAGGGATTGGACTTTGCAGGACAGAGCACATGTTCTTTGCTTCGGATGATAGAATAAAGGCGGTAAGAAAAATGATCATGGCAGCTACAACTGAACAGAGGAAGGCGGCACTGGACCTCTTACTACCGTATCAAAGATATGACTTTGAAGGAATTTTCCGGGCAATGGACGGTCTTCCAGTAACAATCCGCCTGTTAGACCCTCCACTTCATGAATTTCTTCCAGAAGGTGACTTAGAACAGATTGTCAGCGAACTAACTGCAGAGACTGGCATGACTGAGGATGAAGTTTTCTCGAGGATTGAGAAATTATCAGAAGTAAACCCCATGCTTGGTTTCCGCGGCTGCAGGCTAGGGATATCATACCCAGAACTCAGCGAAATGCAGGCACGTGCAATCTTTCAAGCTGCAGTCTCAATGAGCAACCAGGGTGTCAAAGTTTTCCCTGAGATAATGGTTCCCCTTGTCGGAACACCTCAGGCACGATCCTGCAATTCTTGTTCCTCATTTTCTCGTTTCTCGTTATTTACGTATGATACTAGATTCATAATTCATTCGTATGGATTTGAACTAGGACATCAAATGAGGCTCATTCGAAGTGTCGCGGTAAAAGTGTTCTTCGAGATGGGTACTGCCTTGAGTTATAAGGTTGGGACTATGATTGAGATCCCCAGAGCTGCTCTGGTTGCAGATGAGATTGCGAAGGAAGCTGAGTTCTTTTCTTTCGGGACCAATGATCTCACACAAATGACATTTGGCTACAGTAGAGATGATGTGGGCAAGTTTCTACCTATCTACCTTGCGAAAGGCCTTCTTCAAAACGATCCATTTGAGGTCCTTGATCAAAGAGGTGTTGGGCAGCTCATCAAGATGGCCACAGAAAAGGGTCGCGCAGCTAGGCCTAGCTTAAAGGTTGGAATATGCGGAGAGCATGGTGGGGAGCCTTCTTCTGTTGCATTTTTCGCAGAGGCCGGATTAGACTATGTTTCTTGTTCTCCATTCAGGGTACCTATTGCTAGGCTAGCAGCAGCTCAAGTAGCTGTCTGA

Protein Analysis

991

Amino Acids

108.38

Weight (kDa)

5.69

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPDK_N PF01326 107 - 160 1.9e-06 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N PF01326 145 - 379 1.9e-22 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N PF01326 390 - 444 1.2e-10 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PEP-utilizers PF00391 510 - 591 1.2e-22 PEP-utilising enzyme, mobile domain
PEP-utilizers_C PF02896 606 - 802 4.8e-41 PEP-utilising enzyme, PEP-binding domain
PEP-utilizers_C PF02896 832 - 989 3.4e-41 PEP-utilising enzyme, PEP-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2571
Acc65I GGTACC 1 cut(s) 2932
AccB1I GGYRCC 2 cut(s) 1922, 2932
AccI GTMKAC 1 cut(s) 1317
AccII CGCG 6 cut(s) 632, 971, 1883, 2268, 2531, 2817
AclWI GGATC 7 cut(s) 69, 176, 854, 1604, 2406, 2587, 2744
AcoI YGGCCR 2 cut(s) 627, 2799
AcsI RAATTY 5 cut(s) 705, 1076, 1194, 2079, 2141
AcuI CTGAAG 2 cut(s) 840, 1914
AcvI CACGTG 1 cut(s) 2318
AcyI GRCGYC 2 cut(s) 1124, 1861
AfaI GTAC 5 cut(s) 665, 751, 1288, 2557, 2934
AfeI AGCGCT 2 cut(s) 134, 647
AfiI CCNNNNNNNGG 9 cut(s) 1052, 1059, 1119, 1512, 1650, 1651, 2092, 2258, 2393
AflIII ACRYGT 2 cut(s) 681, 1956
AgeI ACCGGT 2 cut(s) 839, 1052
AgsI TTSAA 9 cut(s) 46, 399, 961, 983, 1370, 2075, 2330, 2495, 2744
AhdI GACNNNNNGTC 1 cut(s) 374
AjnI CCWGG 6 cut(s) 199, 345, 382, 568, 1462, 2352
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AloI GAACNNNNNNTCC 4 cut(s) 1217, 1249, 2624, 2656
Alw21I GWGCWC 2 cut(s) 1093, 1956
Alw26I GTCTC 3 cut(s) 1868, 2186, 2344
AlwI GGATC 7 cut(s) 69, 176, 854, 1604, 2406, 2587, 2744
AlwNI CAGNNNCTG 2 cut(s) 788, 2195
Ama87I CYCGRG 1 cut(s) 2221
Aor51HI AGCGCT 2 cut(s) 134, 647
AoxI GGCC 7 cut(s) 100, 124, 627, 2734, 2799, 2825, 2895
ApoI RAATTY 5 cut(s) 705, 1076, 1194, 2079, 2141
AsiGI ACCGGT 2 cut(s) 839, 1052
Asp700I GAANNNNTTC 2 cut(s) 1012, 2145
Asp718I GGTACC 1 cut(s) 2932
AspLEI GCGC 6 cut(s) 135, 648, 1350, 1679, 1903, 2819
AspS9I GGNCC 3 cut(s) 2041, 2655, 2761
AsuC2I CCSGG 1 cut(s) 2087
AsuHPI GGTGA 7 cut(s) 131, 524, 854, 1067, 1336, 1388, 2168
AsuII TTCGAA 1 cut(s) 2521
AsuNHI GCTAGC 2 cut(s) 359, 2947
AvaI CYCGRG 1 cut(s) 2221
AvaII GGWCC 3 cut(s) 2041, 2655, 2761
AxyI CCTNAGG 2 cut(s) 21, 2403
BaeI ACNNNNGTAYC 4 cut(s) 1716, 1749, 2039, 2072
BalI TGGCCA 1 cut(s) 2801
BanI GGYRCC 2 cut(s) 1922, 2932
BanII GRGCYC 1 cut(s) 1797
BbrPI CACGTG 1 cut(s) 2318
BbsI GAAGAC 2 cut(s) 1137, 2093
Bbv12I GWGCWC 2 cut(s) 1093, 1956
BbvCI CCTCAGC 1 cut(s) 1497
BccI CCATC 5 cut(s) 571, 1216, 1357, 2545, 2791
BcgI CGANNNNNNTGC 6 cut(s) 547, 581, 976, 1010, 2168, 2202
BciT130I CCWGG 6 cut(s) 201, 347, 384, 570, 1464, 2354
BciVI GTATCC 2 cut(s) 1132, 1717
BclI TGATCA 2 cut(s) 2001, 2767
BcnI CCSGG 1 cut(s) 2087
BcoDI GTCTC 3 cut(s) 1868, 2186, 2344
BfmI CTRYAG 8 cut(s) 237, 1314, 1473, 1590, 2187, 2272, 2334, 2686
BfoI RGCGCY 2 cut(s) 136, 649
BfuI GTATCC 2 cut(s) 1132, 1717
Bme1390I CCNGG 7 cut(s) 201, 347, 384, 570, 1464, 2087, 2354
Bme18I GGWCC 3 cut(s) 2041, 2655, 2761
BmeRI GACNNNNNGTC 1 cut(s) 374
BmeT110I CYCGRG 1 cut(s) 2221
BmgT120I GGNCC 3 cut(s) 2041, 2655, 2761
BmiI GGNNCC 7 cut(s) 967, 1765, 1924, 2385, 2656, 2868, 2934
BmrFI CCNGG 7 cut(s) 201, 347, 384, 570, 1464, 2087, 2354
BmrI ACTGGG 1 cut(s) 1779
BmsI GCATC 5 cut(s) 141, 948, 1466, 1492, 1888
BmtI GCTAGC 2 cut(s) 363, 2951
BmuI ACTGGG 1 cut(s) 1779
BpiI GAAGAC 2 cut(s) 1137, 2093
BplI GAGNNNNNCTC 4 cut(s) 111, 143, 1734, 1766
BpmI CTGGAG 1 cut(s) 1446
Bpu10I CCTNAGC 2 cut(s) 1092, 1497
Bpu14I TTCGAA 1 cut(s) 2521
BpuEI CTTGAG 4 cut(s) 113, 1850, 2584, 2943
BpuMI CCSGG 1 cut(s) 2087
BsaAI YACGTR 2 cut(s) 2318, 2457
BsaBI GATNNNNATC 2 cut(s) 1226, 2591
BsaHI GRCGYC 2 cut(s) 1124, 1861
BsaJI CCNNGG 6 cut(s) 97, 595, 969, 1645, 2266, 2353
BsaWI WCCGGW 2 cut(s) 839, 1052
BsaXI ACNNNNNCTCC 4 cut(s) 1089, 1119, 1458, 1488
Bsc4I CCNNNNNNNGG 9 cut(s) 1052, 1059, 1119, 1512, 1650, 1651, 2092, 2258, 2393
Bse118I RCCGGY 4 cut(s) 141, 839, 1052, 1112
Bse1I ACTGG 6 cut(s) 1294, 1450, 1774, 2043, 2105, 2200
Bse21I CCTNAGG 2 cut(s) 21, 2403
Bse3DI GCAATG 2 cut(s) 318, 2097
Bse8I GATNNNNATC 2 cut(s) 1226, 2591
BseBI CCWGG 6 cut(s) 201, 347, 384, 570, 1464, 2354
BseDI CCNNGG 6 cut(s) 97, 595, 969, 1645, 2266, 2353
BseGI GGATG 4 cut(s) 1266, 1507, 1978, 2215
BseJI GATNNNNATC 2 cut(s) 1226, 2591
BseLI CCNNNNNNNGG 9 cut(s) 1052, 1059, 1119, 1512, 1650, 1651, 2092, 2258, 2393
BseMI GCAATG 2 cut(s) 318, 2097
BseMII CTCAG 9 cut(s) 12, 1106, 1488, 1692, 2196, 2314, 2364, 2417, 2628
BseNI ACTGG 6 cut(s) 1294, 1450, 1774, 2043, 2105, 2200
BseRI GAGGAG 5 cut(s) 473, 521, 740, 1626, 1794
BseYI CCCAGC 1 cut(s) 232
BsgI GTGCAG 3 cut(s) 234, 506, 1320
Bsh1236I CGCG 6 cut(s) 632, 971, 1883, 2268, 2531, 2817
BshFI GGCC 7 cut(s) 102, 126, 629, 2736, 2801, 2827, 2897
BshNI GGYRCC 2 cut(s) 1922, 2932
BshTI ACCGGT 2 cut(s) 839, 1052
BsiHKAI GWGCWC 2 cut(s) 1093, 1956
BsiHKCI CYCGRG 1 cut(s) 2221
BsiSI CCGG 8 cut(s) 142, 840, 1053, 1113, 1808, 1858, 2086, 2898
BslFI GGGAC 6 cut(s) 214, 362, 923, 1492, 2593, 2668
BslI CCNNNNNNNGG 9 cut(s) 1052, 1059, 1119, 1512, 1650, 1651, 2092, 2258, 2393
BsmAI GTCTC 3 cut(s) 1868, 2186, 2344
BsmBI CGTCTC 1 cut(s) 1868
BsmFI GGGAC 6 cut(s) 214, 362, 923, 1492, 2593, 2668
BsnI GGCC 7 cut(s) 102, 126, 629, 2736, 2801, 2827, 2897
BsoBI CYCGRG 1 cut(s) 2221
Bsp119I TTCGAA 1 cut(s) 2521
Bsp1286I GDGCHC 3 cut(s) 1093, 1797, 1956
Bsp19I CCATGG 1 cut(s) 97
BspANI GGCC 7 cut(s) 102, 126, 629, 2736, 2801, 2827, 2897
BspCNI CTCAG 9 cut(s) 13, 1105, 1489, 1693, 2197, 2313, 2365, 2416, 2629
BspFNI CGCG 6 cut(s) 632, 971, 1883, 2268, 2531, 2817
BspHI TCATGA 2 cut(s) 171, 2137
BspLI GGNNCC 7 cut(s) 967, 1765, 1924, 2385, 2656, 2868, 2934
BspMAI CTGCAG 5 cut(s) 241, 1594, 2191, 2276, 2338
BspOI GCTAGC 2 cut(s) 363, 2951
BspPI GGATC 7 cut(s) 69, 176, 854, 1604, 2406, 2587, 2744
BspT104I TTCGAA 1 cut(s) 2521
BspT107I GGYRCC 2 cut(s) 1922, 2932
BsrDI GCAATG 2 cut(s) 318, 2097
BsrFI RCCGGY 4 cut(s) 141, 839, 1052, 1112
BsrI ACTGG 6 cut(s) 1294, 1450, 1774, 2043, 2105, 2200
BssAI RCCGGY 4 cut(s) 141, 839, 1052, 1112
BssECI CCNNGG 6 cut(s) 97, 595, 969, 1645, 2266, 2353
BssNI GRCGYC 2 cut(s) 1124, 1861
BssT1I CCWWGG 2 cut(s) 97, 595
Bst2UI CCWGG 6 cut(s) 201, 347, 384, 570, 1464, 2354
Bst4CI ACNGT 9 cut(s) 586, 1190, 1240, 1404, 1483, 1729, 2055, 2099, 2690
BstACI GRCGYC 2 cut(s) 1124, 1861
BstAPI GCANNNNNTGC 1 cut(s) 151
BstBAI YACGTR 2 cut(s) 2318, 2457
BstBI TTCGAA 1 cut(s) 2521
BstC8I GCNNGC 9 cut(s) 361, 409, 1114, 1455, 1518, 1584, 2276, 2313, 2949
BstDSI CCRYGG 4 cut(s) 97, 969, 1645, 2266
BstEII GGTNACC 1 cut(s) 512
BstF5I GGATG 4 cut(s) 1266, 1507, 1978, 2215
BstFNI CGCG 6 cut(s) 632, 971, 1883, 2268, 2531, 2817
BstH2I RGCGCY 2 cut(s) 136, 649
BstHHI GCGC 6 cut(s) 135, 648, 1350, 1679, 1903, 2819
BstMAI GTCTC 3 cut(s) 1868, 2186, 2344
BstNI CCWGG 6 cut(s) 201, 347, 384, 570, 1464, 2354
BstNSI RCATGY 5 cut(s) 411, 685, 1520, 1586, 1960
BstPI GGTNACC 1 cut(s) 512
BstSCI CCNGG 7 cut(s) 199, 345, 382, 568, 1462, 2085, 2352
BstSFI CTRYAG 8 cut(s) 237, 1314, 1473, 1590, 2187, 2272, 2334, 2686
BstSNI TACGTA 1 cut(s) 2457
BstUI CGCG 6 cut(s) 632, 971, 1883, 2268, 2531, 2817
BstV2I GAAGAC 2 cut(s) 1137, 2093
BstX2I RGATCY 3 cut(s) 859, 1596, 2592
BstYI RGATCY 3 cut(s) 859, 1596, 2592
Bsu36I CCTNAGG 2 cut(s) 21, 2403
BsuI GTATCC 2 cut(s) 1132, 1717
BsuRI GGCC 7 cut(s) 102, 126, 629, 2736, 2801, 2827, 2897
BtgI CCRYGG 4 cut(s) 97, 969, 1645, 2266
BtsCI GGATG 4 cut(s) 1266, 1507, 1978, 2215
BtsI GCAGTG 3 cut(s) 1282, 1308, 1350
Cac8I GCNNGC 9 cut(s) 361, 409, 1114, 1455, 1518, 1584, 2276, 2313, 2949
CaiI CAGNNNCTG 2 cut(s) 788, 2195
CciI TCATGA 2 cut(s) 171, 2137
CfoI GCGC 6 cut(s) 135, 648, 1350, 1679, 1903, 2819
Cfr10I RCCGGY 4 cut(s) 141, 839, 1052, 1112
Cfr13I GGNCC 3 cut(s) 2041, 2655, 2761
Cfr42I CCGCGG 2 cut(s) 972, 2269
CseI GACGC 3 cut(s) 1132, 1706, 1850
Csp6I GTAC 5 cut(s) 664, 750, 1287, 2556, 2933
CspAI ACCGGT 2 cut(s) 839, 1052
CspCI CAANNNNNGTGG 2 cut(s) 434, 469
CviQI GTAC 5 cut(s) 664, 750, 1287, 2556, 2933
DriI GACNNNNNGTC 1 cut(s) 374
EaeI YGGCCR 2 cut(s) 627, 2799
Eam1105I GACNNNNNGTC 1 cut(s) 374
EciI GGCGGA 1 cut(s) 2105
Eco105I TACGTA 1 cut(s) 2457
Eco130I CCWWGG 2 cut(s) 97, 595
Eco147I AGGCCT 2 cut(s) 2736, 2827
Eco24I GRGCYC 1 cut(s) 1797
Eco32I GATATC 3 cut(s) 1228, 1684, 2286
Eco47I GGWCC 3 cut(s) 2041, 2655, 2761
Eco47III AGCGCT 2 cut(s) 134, 647
Eco57I CTGAAG 2 cut(s) 840, 1914
Eco72I CACGTG 1 cut(s) 2318
Eco81I CCTNAGG 2 cut(s) 21, 2403
Eco88I CYCGRG 1 cut(s) 2221
Eco91I GGTNACC 1 cut(s) 512
EcoO109I RGGNCCY 1 cut(s) 2761
EcoO65I GGTNACC 1 cut(s) 512
EcoRI GAATTC 1 cut(s) 705
EcoRII CCWGG 6 cut(s) 199, 345, 382, 568, 1462, 2352
EcoRV GATATC 3 cut(s) 1228, 1684, 2286
EcoT14I CCWWGG 2 cut(s) 97, 595
EcoT22I ATGCAT 2 cut(s) 989, 1584
EcoT38I GRGCYC 1 cut(s) 1797
ErhI CCWWGG 2 cut(s) 97, 595
Esp3I CGTCTC 1 cut(s) 1868
FalI AAGNNNNNCTT 4 cut(s) 1782, 1814, 2724, 2756
FaqI GGGAC 6 cut(s) 214, 362, 923, 1492, 2593, 2668
FbaI TGATCA 2 cut(s) 2001, 2767
FblI GTMKAC 1 cut(s) 1317
FokI GGATG 4 cut(s) 1273, 1514, 1985, 2222
FriOI GRGCYC 1 cut(s) 1797
GlaI GCGC 6 cut(s) 134, 647, 1349, 1678, 1902, 2818
GsaI CCCAGC 1 cut(s) 236
GsuI CTGGAG 1 cut(s) 1446
HaeII RGCGCY 2 cut(s) 136, 649
HaeIII GGCC 7 cut(s) 102, 126, 629, 2736, 2801, 2827, 2897
HapII CCGG 8 cut(s) 142, 840, 1053, 1113, 1808, 1858, 2086, 2898
HgaI GACGC 3 cut(s) 1132, 1706, 1850
HhaI GCGC 6 cut(s) 135, 648, 1350, 1679, 1903, 2819
Hin1I GRCGYC 2 cut(s) 1124, 1861
Hin6I GCGC 6 cut(s) 133, 646, 1348, 1677, 1901, 2817
HinP1I GCGC 6 cut(s) 133, 646, 1348, 1677, 1901, 2817
HindIII AAGCTT 2 cut(s) 1064, 1164
HinfI GANTC 6 cut(s) 600, 659, 902, 1042, 1411, 2469
HpaII CCGG 8 cut(s) 142, 840, 1053, 1113, 1808, 1858, 2086, 2898
HphI GGTGA 7 cut(s) 131, 524, 854, 1067, 1336, 1388, 2168
Hpy166II GTNNAC 6 cut(s) 163, 1035, 1236, 1318, 1327, 2248
Hpy8I GTNNAC 6 cut(s) 163, 1035, 1236, 1318, 1327, 2248
Hpy99I CGWCG 1 cut(s) 115
HpyCH4III ACNGT 9 cut(s) 586, 1190, 1240, 1404, 1483, 1729, 2055, 2099, 2690
HpyCH4IV ACGT 3 cut(s) 375, 2317, 2456
HpySE526I ACGT 3 cut(s) 375, 2317, 2456
Hsp92I GRCGYC 2 cut(s) 1124, 1861
HspAI GCGC 6 cut(s) 133, 646, 1348, 1677, 1901, 2817
KpnI GGTACC 1 cut(s) 2936
KroI GCCGGC 1 cut(s) 1112
KroNI GCCGGC 1 cut(s) 1114
Ksp22I TGATCA 2 cut(s) 2001, 2767
KspI CCGCGG 2 cut(s) 972, 2269
LmnI GCTCC 5 cut(s) 727, 936, 1174, 1466, 2866
LweI GCATC 5 cut(s) 141, 948, 1466, 1492, 1888
MaeII ACGT 3 cut(s) 375, 2317, 2456
MaeIII GTNAC 5 cut(s) 137, 512, 1263, 2107, 2156
MflI RGATCY 3 cut(s) 859, 1596, 2592
MhlI GDGCHC 3 cut(s) 1093, 1797, 1956
MlsI TGGCCA 1 cut(s) 2801
MluNI TGGCCA 1 cut(s) 2801
MlyI GAGTC 2 cut(s) 594, 653
MmeI TCCRAC 4 cut(s) 714, 1439, 2374, 2821
Mox20I TGGCCA 1 cut(s) 2801
Mph1103I ATGCAT 2 cut(s) 989, 1584
MroNI GCCGGC 1 cut(s) 1112
MroXI GAANNNNTTC 2 cut(s) 1012, 2145
MscI TGGCCA 1 cut(s) 2801
MseI TTAA 5 cut(s) 888, 975, 1082, 1814, 2834
MslI CAYNNNNRTG 3 cut(s) 2484, 2672, 2795
Msp20I TGGCCA 1 cut(s) 2801
MspA1I CMGCKG 7 cut(s) 236, 424, 971, 1472, 1589, 1634, 2268
MspI CCGG 8 cut(s) 142, 840, 1053, 1113, 1808, 1858, 2086, 2898
MspR9I CCNGG 7 cut(s) 201, 347, 384, 570, 1464, 2087, 2354
MvaI CCWGG 6 cut(s) 201, 347, 384, 570, 1464, 2354
MvnI CGCG 6 cut(s) 632, 971, 1883, 2268, 2531, 2817
NaeI GCCGGC 1 cut(s) 1114
NciI CCSGG 1 cut(s) 2087
NcoI CCATGG 1 cut(s) 97
NgoMIV GCCGGC 1 cut(s) 1112
NheI GCTAGC 2 cut(s) 359, 2947
NlaIV GGNNCC 7 cut(s) 967, 1765, 1924, 2385, 2656, 2868, 2934
NmuCI GTSAC 3 cut(s) 137, 512, 2156
NsiI ATGCAT 2 cut(s) 989, 1584
NspI RCATGY 5 cut(s) 411, 685, 1520, 1586, 1960
NspV TTCGAA 1 cut(s) 2521
PaeI GCATGC 3 cut(s) 411, 1520, 1586
PaeR7I CTCGAG 1 cut(s) 2221
PagI TCATGA 2 cut(s) 171, 2137
PceI AGGCCT 2 cut(s) 2736, 2827
PciI ACATGT 2 cut(s) 681, 1956
PcsI WCGNNNNNNNCGW 1 cut(s) 2453
PdiI GCCGGC 1 cut(s) 1114
PdmI GAANNNNTTC 2 cut(s) 1012, 2145
PfeI GAWTC 4 cut(s) 902, 1042, 1411, 2469
PflFI GACNNNGTC 1 cut(s) 584
PfoI TCCNGGA 1 cut(s) 1462
PinAI ACCGGT 2 cut(s) 839, 1052
PleI GAGTC 2 cut(s) 594, 653
PmaCI CACGTG 1 cut(s) 2318
PmlI CACGTG 1 cut(s) 2318
PpsI GAGTC 2 cut(s) 594, 653
Ppu21I YACGTR 2 cut(s) 2318, 2457
PpuMI RGGWCCY 1 cut(s) 2761
PscI ACATGT 2 cut(s) 681, 1956
PsiI TTATAA 1 cut(s) 2571
Psp5II RGGWCCY 1 cut(s) 2761
Psp6I CCWGG 6 cut(s) 199, 345, 382, 568, 1462, 2352
PspCI CACGTG 1 cut(s) 2318
PspEI GGTNACC 1 cut(s) 512
PspFI CCCAGC 1 cut(s) 232
PspGI CCWGG 6 cut(s) 199, 345, 382, 568, 1462, 2352
PspN4I GGNNCC 7 cut(s) 967, 1765, 1924, 2385, 2656, 2868, 2934
PspPI GGNCC 3 cut(s) 2041, 2655, 2761
PspPPI RGGWCCY 1 cut(s) 2761
PstI CTGCAG 5 cut(s) 241, 1594, 2191, 2276, 2338
PstNI CAGNNNCTG 2 cut(s) 788, 2195
PsuI RGATCY 3 cut(s) 859, 1596, 2592
PsyI GACNNNGTC 1 cut(s) 584
PvuII CAGCTG 5 cut(s) 236, 424, 1472, 1589, 1634
RsaI GTAC 5 cut(s) 665, 751, 1288, 2557, 2934
RsaNI GTAC 5 cut(s) 664, 750, 1287, 2556, 2933
RseI CAYNNNNRTG 3 cut(s) 2484, 2672, 2795
SacII CCGCGG 2 cut(s) 972, 2269
SaqAI TTAA 5 cut(s) 888, 975, 1082, 1814, 2834
Sau96I GGNCC 3 cut(s) 2041, 2655, 2761
SchI GAGTC 2 cut(s) 594, 653
ScrFI CCNGG 7 cut(s) 201, 347, 384, 570, 1464, 2087, 2354
SduI GDGCHC 3 cut(s) 1093, 1797, 1956
SfaNI GCATC 5 cut(s) 141, 948, 1466, 1492, 1888
SfcI CTRYAG 8 cut(s) 237, 1314, 1473, 1590, 2187, 2272, 2334, 2686
Sfr274I CTCGAG 1 cut(s) 2221
Sfr303I CCGCGG 2 cut(s) 972, 2269
SfuI TTCGAA 1 cut(s) 2521
SgrAI CRCCGGYG 2 cut(s) 839, 1052
SgrBI CCGCGG 2 cut(s) 972, 2269
SinI GGWCC 3 cut(s) 2041, 2655, 2761
SlaI CTCGAG 1 cut(s) 2221
SmiMI CAYNNNNRTG 3 cut(s) 2484, 2672, 2795
SmlI CTYRAG 5 cut(s) 128, 1865, 2221, 2563, 2958
SmoI CTYRAG 5 cut(s) 128, 1865, 2221, 2563, 2958
SnaBI TACGTA 1 cut(s) 2457
SphI GCATGC 3 cut(s) 411, 1520, 1586
SseBI AGGCCT 2 cut(s) 2736, 2827
SspI AATATT 1 cut(s) 979
StuI AGGCCT 2 cut(s) 2736, 2827
StyD4I CCNGG 7 cut(s) 199, 345, 382, 568, 1462, 2085, 2352
StyI CCWWGG 2 cut(s) 97, 595
TaaI ACNGT 9 cut(s) 586, 1190, 1240, 1404, 1483, 1729, 2055, 2099, 2690
TaiI ACGT 3 cut(s) 378, 2320, 2459
TaqI TCGA 8 cut(s) 118, 210, 831, 1230, 1734, 2222, 2521, 2547
TauI GCSGC 3 cut(s) 632, 2036, 2271
TfiI GAWTC 4 cut(s) 902, 1042, 1411, 2469
Tru1I TTAA 5 cut(s) 888, 975, 1082, 1814, 2834
Tru9I TTAA 5 cut(s) 888, 975, 1082, 1814, 2834
TseFI GTSAC 3 cut(s) 137, 512, 2156
Tsp45I GTSAC 3 cut(s) 137, 512, 2156
TspDTI ATGAA 9 cut(s) 188, 329, 1160, 1825, 2126, 2154, 2226, 2461, 2468
TspGWI ACGGA 1 cut(s) 529
Tth111I GACNNNGTC 1 cut(s) 584
VpaK11BI GGWCC 3 cut(s) 2041, 2655, 2761
XapI RAATTY 5 cut(s) 705, 1076, 1194, 2079, 2141
XbaI TCTAGA 2 cut(s) 676, 1198
XceI RCATGY 5 cut(s) 411, 685, 1520, 1586, 1960
XcmI CCANNNNNNNNNTGG 1 cut(s) 2605
XhoI CTCGAG 1 cut(s) 2221
XmiI GTMKAC 1 cut(s) 1317
XmnI GAANNNNTTC 2 cut(s) 1012, 2145
Zsp2I ATGCAT 2 cut(s) 989, 1584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.