Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0004385

Physical Location & Seq

Genomic Coordinates Forward (+)

42425290 .. 42431912

Gene Structure 6623 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G019220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2883 bp

ATGTCATCCACAGTGAAAGGTTTACTCTTGAGGACTTCACCGGAGGTAGCTTATAGACACAGAATGTTCAACGATCGTTCTTTCCTCAACGGCGTGAACGGTGGCTCCACCCAAGCCGGCCAGACCCGGCATCAGCCTCACGTTGCTACCATAACAAATGGGATCACCAACCCCAGTCCTAAGAAAAATGAGCTGCAGAGCCAGATCGGAGCCCAGCCTATGATGTCACCAGTTTTGGACCCGAGTACTCCAACAACGCACAAGAGGGTTTTTACTTTTGGGAAGGGAAGGAGTGAAGGAAACAAGGGCATGAAGTCACTGTTGGGAGGGAAGGGTGCAAACCTGGCTGAAATGGCTAGCATCGGGCTATCCGTGCCACCTGGGCTTACTATCTCAACAGAAGCATGCCAAGAGTATCAGGAGAATGGTAAAGAACTACCGGAAGGTTTGTGGGAGGAGATACTAGAAGGCTTGGATTATGTGCAGAAGACTATGGGAGCTACTCTTGGTGACCCTTCCAAGCCCCTCCTTGTCTCTGTTCGCTCTGGTGCAGCGATTTCTATGCCTGGCATGATGGACACTGTCCTCAACCTTGGACTGAATGATGAGGTGGTTGCTGGTTTAGCTGTAAAAAGTGGGGAGCGCTTTGCATATGACTCATACAGACGTTTCCTAGACATGTTTGGAGATGTTGTTATGGAAATTCCACACTCATCATTTGGGGAGAAGTTGGAAAACTACAAACACCAAAAAGGAGTTGAACTTGATACAGAGCTAACAGCTTCTGATCTCAAAGAGCTGGTAGAACAATACAAAAGTGTCTATCTTGAAGCTACGGGTCAAAAATTCCCTTCAGATCCAAAACAACAGCTAGTGTTGGCTATTAAAGCAGTTTTTAATTCTTGGGACAGCCCGAGGGCCAACAAATACCGGAGCATCAATCAGATAACTGGTTTAAAGGGAACTGCAGTTAACATTCAAAGCATGGCTTTTGGGAACATGGGAAGCACTTCAGGGACAGGTGTTCTATTCACTAGAAATCCAAGCACTGGTGAAAAGAAGCTCTATGGGGAGTTTCTAGTCAATGCTCAGGGAGAAGATGTAGTTGCCGGTATTAGGACACCAGAACCTTTGGATACCATGAAAAATTGCATGCCAGAAGCTTACAAGGAGCTTGTGGAGAACTGTGAAATTCTAGAAAGACATTACAAAGATATGATGGACATTGAGTTCACTGTCCAAGAAAATAGATTATGGATGTTGCAATGTCGTACTGGAAAGCGTACTGGTAAAGGTGCGATCAAGATAGCTGTAGACATGGTTAATGAAGGGCTTGTTGAAAAGCGCTCCGCAATCAAGATGGTGGAACCACAACATGTTGACCAACTTCTACACCCTCAGTTTGAGGATCCGACAGCATACAAAGACAAAGTACTTGCCACTGGCTTGCCTGCATCACCAGGAGCAGCAGTAGGGCAAATCGTGTTCAGTGCAGAGGACGCTGAATCATGGCATGCACAAGGAAAGAAGACCATTTTGGTAAGGACGGAAACCAGCCCGGAAGATATTGGGGGAATGCATGCATCTGCTGGGATCTTGACTGCTAGAGGTGGCATGACATCTCACGCAGCTGTTGTCGCTCGTGGATGGGGTAAATGTTGTGTTTCTGGATGCTCTGATATCCGAGTAAATGATGATGAGAAGGTTGTTGTGATTGGGGACACTTTGTTTAGAGAAGGAGAATGGCTCTCCCTCAATGGAAACACCGGTGAAGTCATATCAGGAAAACAGCCACTTTCTCCTCCAGCTTTAAGCGGTGATTTGGAAACCTTTATGTCTTGGGCTGATAGCTTTAGGCGTCTCAAGGTTATGGCAAATGCCGACACACCTGAAGATGCACTAACAGCAAGAAACAATGGTGCACAAGGGATTGGACTTTGCAGGACAGAGCACATGTTCTTTGCTTCAGAGGAAAGAATTAAGTCAGTAAGAAAGATGATCATGGCAAATACACTTGAGCAGAGGAAGGCAGCATTGGATCTCTTACTACCATATCAGAGATCTGACTTTGAAGGAATTTTCCGTGCAATGGATGGTCTTCCGGTAACCATCCGATTGTTAGACCCTCCACTTCATGAGTTTCTTCCAGAAGGTGACCTAGAACAGATTGTTAAAGAAGTAGCTGCAGAGACTGACATGACTGAGGATGATGTTTACTCAAGAGTTGATAAATTAACAGAAGTAAACCCAATGCTTGGTTTCCGGGGCTGCAGGCTAGGGATTTCGTACCCAGAACTCACAGAAATGCAAGCACGTGCAGTCTTTCAGGCTGCAGTCTTGATGAGCAACCAGGGTGTCAAAGTTTTCCCTGAGATAATGGTTCCTCTTGTTGGATCACCTCAGGAACTGGGACACCAAGTGACTTTAATCCGGAGTGTTGCAAAGAAAGTGTTCTCTGAAATGGGTACTACCTTGAGTTTCAAGGTTGGAACCATGATTGAGATTCCCAGAGCTGCTCTGATTGCAGATGAGATTGCAAAAGAAGCAGAGTTCTTCTCTTTTGGAACCAATGATCTCACCCAAATGACCTTTGGGTACAGTAGAGATGATGTGGGCAAGTTCCTACCAATTTATTTGTCCAAAGGCATTCTTCAAAATGATCCATTTGAGGTACTGGATCAAAGAGGAGTTGGGCAGCTCATCAAGATGGCCACAGAAAAGGGTCGTGCAGCTAAGCCAAGCTTAAAGGTTGGAATATGCGGTGAGCATGGTGGGGAACCTTCTTCTGTTGCATTTTTCGCTGAGGCCGGACTAGACTATGTTTCATGTTCTCCATTCAGGGTACCTATTGCAAGGCTAGCAGCAGCTCAAGTTGCCGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko00720 ko01100 ko01120 ko01200 map00620 map00710 map00720 map01100 map01120 map01200

Pfam Domains

Protein Families

PEP-utilizers PEP-utilizers_C PPDK_N

Protein Analysis

960

Amino Acids

104.74

Weight (kDa)

5.57

Isoelectric Point (pI)

37.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPDK_N	PF01326	106 - 142	7.2e-07	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N	PF01326	144 - 377	3.5e-23	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N	PF01326	388 - 442	5.6e-10	Pyruvate phosphate dikinase, AMP/ATP-binding domain
PEP-utilizers	PF00391	509 - 590	2e-22	PEP-utilising enzyme, mobile domain
PEP-utilizers_C	PF02896	605 - 959	3.5e-92	PEP-utilising enzyme, PEP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0004385)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530 AT4G15530
fragaria_vesca	FvH4_4g16670
malus_domestica	MD13G1177400.v1.1 MD13G1177500.v1.1 MD16G1179300.v1.1 MD16G1179400.v1.1 MD16G1179500.v1.1
prunus_persica	Prupe.1G146600_v2.0.a1
pyrus_communis	pycom13g15300 pycom16g15050 pycom16g15060 pycom16g15070
rosa_chinensis	RchiOBHm_Chr4g0420131
rosa_laevigata	RLG00000007786
rosa_multiflora	Rmu_sc0001252.1_g000003 Rmu_sc0025222.1_g000001
rosa_roxburghii	Rroxscaffold_5G00362840
rosa_rugosa	Rorug04G0162400 Rorug04G0162500 Rorug04G0162600 Rorug04G0162600
rosa_samantha	Rh4AG223300 Rh4BG225700 Rh4CG236500 Rh4DG222100
rosa_wichuraiana	Rw4G019220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	2842
AccB1I	GGYRCC	1 cut(s)	2842
AccB7I	CCANNNNNTGG	2 cut(s)	1049, 2255
AccI	GTMKAC	1 cut(s)	1314
AccIII	TCCGGA	1 cut(s)	2430
AciI	CCGC	3 cut(s)	1350, 1815, 2760
AclWI	GGATC	9 cut(s)	170, 851, 1403, 1416, 1601, 2046, 2401, 2654, 2685
AcoI	YGGCCR	2 cut(s)	118, 2709
AcsI	RAATTY	4 cut(s)	702, 845, 1191, 2076
AcuI	CTGAAG	4 cut(s)	837, 996, 1911, 1950
AcvI	CACGTG	1 cut(s)	2315
AcyI	GRCGYC	1 cut(s)	1858
AdeI	CACNNNGTG	1 cut(s)	2419
AfaI	GTAC	9 cut(s)	247, 1273, 1285, 1434, 2288, 2467, 2597, 2673, 2844
AfeI	AGCGCT	2 cut(s)	644, 1346
AfiI	CCNNNNNNNGG	5 cut(s)	1049, 1116, 2089, 2255, 2390
AflIII	ACRYGT	3 cut(s)	678, 1375, 1953
AgeI	ACCGGT	1 cut(s)	1766
AgsI	TTSAA	8 cut(s)	70, 761, 830, 980, 1340, 2072, 2482, 2654
AjnI	CCWGG	5 cut(s)	342, 379, 565, 1459, 2349
AjuI	GAANNNNNNNTTGG	6 cut(s)	305, 337, 1520, 1552, 2018, 2050
AloI	GAACNNNNNNTCC	4 cut(s)	89, 121, 1214, 1246
Alw21I	GWGCWC	2 cut(s)	1924, 1953
Alw26I	GTCTC	3 cut(s)	538, 1865, 2183
Alw44I	GTGCAC	1 cut(s)	1920
AlwI	GGATC	9 cut(s)	170, 851, 1403, 1416, 1601, 2046, 2401, 2654, 2685
AlwNI	CAGNNNCTG	3 cut(s)	785, 2192, 2407
Ama87I	CYCGRG	2 cut(s)	241, 913
Aor13HI	TCCGGA	1 cut(s)	2430
Aor51HI	AGCGCT	2 cut(s)	644, 1346
AoxI	GGCC	4 cut(s)	118, 918, 2709, 2805
ApaLI	GTGCAC	1 cut(s)	1920
ApoI	RAATTY	4 cut(s)	702, 845, 1191, 2076
AsiGI	ACCGGT	1 cut(s)	1766
Asp700I	GAANNNNTTC	2 cut(s)	1009, 2450
Asp718I	GGTACC	1 cut(s)	2842
AspLEI	GCGC	2 cut(s)	645, 1347
AspS9I	GGNCC	2 cut(s)	238, 918
AsuC2I	CCSGG	3 cut(s)	127, 1559, 2264
AsuNHI	GCTAGC	2 cut(s)	356, 2857
AvaI	CYCGRG	2 cut(s)	241, 913
AvaII	GGWCC	1 cut(s)	238
AxyI	CCTNAGG	1 cut(s)	2400
BaeGI	GKGCMC	1 cut(s)	1924
BalI	TGGCCA	1 cut(s)	2711
BamHI	GGATCC	1 cut(s)	1408
BanI	GGYRCC	1 cut(s)	2842
BanII	GRGCYC	1 cut(s)	214
BauI	CACGAG	1 cut(s)	1641
BbrPI	CACGTG	1 cut(s)	2315
BbsI	GAAGAC	3 cut(s)	494, 1535, 2090
Bbv12I	GWGCWC	2 cut(s)	1924, 1953
BbvCI	CCTCAGC	1 cut(s)	2802
BccI	CCATC	7 cut(s)	568, 1213, 1354, 1641, 2087, 2117, 2701
BceAI	ACGGC	2 cut(s)	106, 2861
BcgI	CGANNNNNNTGC	2 cut(s)	544, 578
BciT130I	CCWGG	5 cut(s)	344, 381, 567, 1461, 2351
BciVI	GTATCC	1 cut(s)	1129
BclI	TGATCA	1 cut(s)	1998
BcnI	CCSGG	3 cut(s)	127, 1559, 2264
BcoDI	GTCTC	3 cut(s)	538, 1865, 2183
BfmI	CTRYAG	6 cut(s)	194, 966, 1311, 2184, 2269, 2331
BfoI	RGCGCY	2 cut(s)	646, 1348
BfuI	GTATCC	1 cut(s)	1129
BglI	GCCNNNNNGGC	1 cut(s)	382
BglII	AGATCT	1 cut(s)	2060
BlpI	GCTNAGC	1 cut(s)	2733
BmcAI	AGTACT	2 cut(s)	247, 1434
Bme1390I	CCNGG	8 cut(s)	127, 344, 381, 567, 1461, 1559, 2264, 2351
Bme18I	GGWCC	1 cut(s)	238
BmeT110I	CYCGRG	2 cut(s)	241, 913
BmgT120I	GGNCC	2 cut(s)	238, 918
BmrFI	CCNGG	8 cut(s)	127, 344, 381, 567, 1461, 1559, 2264, 2351
BmrI	ACTGGG	2 cut(s)	168, 2417
BmsI	GCATC	7 cut(s)	139, 369, 945, 1463, 1592, 1661, 1885
BmtI	GCTAGC	2 cut(s)	360, 2861
BmuI	ACTGGG	2 cut(s)	168, 2417
BpiI	GAAGAC	3 cut(s)	494, 1535, 2090
BplI	GAGNNNNNCTC	4 cut(s)	1731, 1763, 2540, 2572
BpmI	CTGGAG	1 cut(s)	1788
Bpu10I	CCTNAGC	2 cut(s)	1089, 2802
Bpu1102I	GCTNAGC	1 cut(s)	2733
BpuEI	CTTGAG	6 cut(s)	49, 1847, 2036, 2203, 2494, 2853
BpuMI	CCSGG	3 cut(s)	127, 1559, 2264
BsaAI	YACGTR	1 cut(s)	2315
BsaHI	GRCGYC	1 cut(s)	1858
BsaJI	CCNNGG	5 cut(s)	380, 592, 914, 2263, 2350
BsaWI	WCCGGW	6 cut(s)	40, 439, 930, 1766, 2101, 2430
BsaXI	ACNNNNNCTCC	8 cut(s)	89, 119, 678, 708, 1086, 1116, 1455, 1485
Bsc4I	CCNNNNNNNGG	5 cut(s)	1049, 1116, 2089, 2255, 2390
Bse118I	RCCGGY	3 cut(s)	116, 1109, 1766
Bse1I	ACTGG	9 cut(s)	174, 230, 955, 1054, 1279, 1291, 1447, 2412, 2679
Bse21I	CCTNAGG	1 cut(s)	2400
Bse3DI	GCAATG	2 cut(s)	1271, 2094
BseAI	TCCGGA	1 cut(s)	2430
BseBI	CCWGG	5 cut(s)	344, 381, 567, 1461, 2351
BseDI	CCNNGG	5 cut(s)	380, 592, 914, 2263, 2350
BseGI	GGATG	7 cut(s)	5, 1263, 1652, 1676, 2098, 2109, 2212
BseLI	CCNNNNNNNGG	5 cut(s)	1049, 1116, 2089, 2255, 2390
BseMI	GCAATG	2 cut(s)	1271, 2094
BseMII	CTCAG	6 cut(s)	1103, 1412, 2193, 2361, 2414, 2793
BseNI	ACTGG	9 cut(s)	174, 230, 955, 1054, 1279, 1291, 1447, 2412, 2679
BseRI	GAGGAG	3 cut(s)	470, 1791, 2700
BseSI	GKGCMC	1 cut(s)	1924
BseYI	CCCAGC	2 cut(s)	213, 1589
BsgI	GTGCAG	5 cut(s)	503, 570, 1512, 2337, 2748
Bsh1285I	CGRYCG	1 cut(s)	76
BshFI	GGCC	4 cut(s)	120, 920, 2711, 2807
BshNI	GGYRCC	1 cut(s)	2842
BshTI	ACCGGT	1 cut(s)	1766
BsiEI	CGRYCG	1 cut(s)	76
BsiHKAI	GWGCWC	2 cut(s)	1924, 1953
BsiHKCI	CYCGRG	2 cut(s)	241, 913
BslFI	GGGAC	4 cut(s)	920, 1030, 1733, 2424
BslI	CCNNNNNNNGG	5 cut(s)	1049, 1116, 2089, 2255, 2390
BsmAI	GTCTC	3 cut(s)	538, 1865, 2183
BsmBI	CGTCTC	1 cut(s)	1865
BsmFI	GGGAC	4 cut(s)	920, 1030, 1733, 2424
BsmI	GAATGC	2 cut(s)	1581, 2646
BsnI	GGCC	4 cut(s)	120, 920, 2711, 2807
BsoBI	CYCGRG	2 cut(s)	241, 913
Bsp1286I	GDGCHC	3 cut(s)	214, 1924, 1953
Bsp13I	TCCGGA	1 cut(s)	2430
Bsp1720I	GCTNAGC	1 cut(s)	2733
BspACI	CCGC	3 cut(s)	1350, 1815, 2760
BspANI	GGCC	4 cut(s)	120, 920, 2711, 2807
BspCNI	CTCAG	6 cut(s)	1102, 1411, 2194, 2362, 2413, 2794
BspEI	TCCGGA	1 cut(s)	2430
BspHI	TCATGA	1 cut(s)	2134
BspMAI	CTGCAG	5 cut(s)	198, 970, 2188, 2273, 2335
BspOI	GCTAGC	2 cut(s)	360, 2861
BspPI	GGATC	9 cut(s)	170, 851, 1403, 1416, 1601, 2046, 2401, 2654, 2685
BspT107I	GGYRCC	1 cut(s)	2842
BsrDI	GCAATG	2 cut(s)	1271, 2094
BsrFI	RCCGGY	3 cut(s)	116, 1109, 1766
BsrI	ACTGG	9 cut(s)	174, 230, 955, 1054, 1279, 1291, 1447, 2412, 2679
BssAI	RCCGGY	3 cut(s)	116, 1109, 1766
BssECI	CCNNGG	5 cut(s)	380, 592, 914, 2263, 2350
BssNI	GRCGYC	1 cut(s)	1858
BssSI	CACGAG	1 cut(s)	1641
BssT1I	CCWWGG	1 cut(s)	592
Bst2BI	CACGAG	1 cut(s)	1641
Bst2UI	CCWGG	5 cut(s)	344, 381, 567, 1461, 2351
Bst4CI	ACNGT	7 cut(s)	13, 101, 321, 583, 1187, 1237, 2600
BstACI	GRCGYC	1 cut(s)	1858
BstBAI	YACGTR	1 cut(s)	2315
BstDEI	CTNAG	8 cut(s)	180, 1089, 1398, 2202, 2370, 2400, 2733, 2802
BstEII	GGTNACC	3 cut(s)	509, 2104, 2153
BstF5I	GGATG	7 cut(s)	5, 1263, 1652, 1676, 2098, 2109, 2212
BstH2I	RGCGCY	2 cut(s)	646, 1348
BstHHI	GCGC	2 cut(s)	645, 1347
BstMAI	GTCTC	3 cut(s)	538, 1865, 2183
BstMCI	CGRYCG	1 cut(s)	76
BstNI	CCWGG	5 cut(s)	344, 381, 567, 1461, 2351
BstNSI	RCATGY	7 cut(s)	408, 682, 1156, 1379, 1517, 1583, 1957
BstPI	GGTNACC	3 cut(s)	509, 2104, 2153
BstSCI	CCNGG	8 cut(s)	125, 342, 379, 565, 1459, 1557, 2262, 2349
BstSFI	CTRYAG	6 cut(s)	194, 966, 1311, 2184, 2269, 2331
BstSLI	GKGCMC	1 cut(s)	1924
BstV2I	GAAGAC	3 cut(s)	494, 1535, 2090
BstX2I	RGATCY	5 cut(s)	856, 1408, 1593, 2038, 2060
BstYI	RGATCY	5 cut(s)	856, 1408, 1593, 2038, 2060
Bsu36I	CCTNAGG	1 cut(s)	2400
BsuI	GTATCC	1 cut(s)	1129
BsuRI	GGCC	4 cut(s)	120, 920, 2711, 2807
BtsCI	GGATG	7 cut(s)	5, 1263, 1652, 1676, 2098, 2109, 2212
BtsIMutI	CAGTG	7 cut(s)	18, 317, 579, 1047, 1233, 1440, 1495
CaiI	CAGNNNCTG	3 cut(s)	785, 2192, 2407
CciI	TCATGA	1 cut(s)	2134
CfoI	GCGC	2 cut(s)	645, 1347
Cfr10I	RCCGGY	3 cut(s)	116, 1109, 1766
Cfr13I	GGNCC	2 cut(s)	238, 918
CseI	GACGC	2 cut(s)	1508, 1847
Csp6I	GTAC	9 cut(s)	246, 1272, 1284, 1433, 2287, 2466, 2596, 2672, 2843
CspAI	ACCGGT	1 cut(s)	1766
CviQI	GTAC	9 cut(s)	246, 1272, 1284, 1433, 2287, 2466, 2596, 2672, 2843
DdeI	CTNAG	8 cut(s)	180, 1089, 1398, 2202, 2370, 2400, 2733, 2802
DraI	TTTAAA	1 cut(s)	957
DraIII	CACNNNGTG	1 cut(s)	2419
EaeI	YGGCCR	2 cut(s)	118, 2709
Eco130I	CCWWGG	1 cut(s)	592
Eco24I	GRGCYC	1 cut(s)	214
Eco32I	GATATC	1 cut(s)	1681
Eco47I	GGWCC	1 cut(s)	238
Eco47III	AGCGCT	2 cut(s)	644, 1346
Eco57I	CTGAAG	4 cut(s)	837, 996, 1911, 1950
Eco72I	CACGTG	1 cut(s)	2315
Eco81I	CCTNAGG	1 cut(s)	2400
Eco88I	CYCGRG	2 cut(s)	241, 913
Eco91I	GGTNACC	3 cut(s)	509, 2104, 2153
EcoO65I	GGTNACC	3 cut(s)	509, 2104, 2153
EcoRII	CCWGG	5 cut(s)	342, 379, 565, 1459, 2349
EcoRV	GATATC	1 cut(s)	1681
EcoT14I	CCWWGG	1 cut(s)	592
EcoT22I	ATGCAT	2 cut(s)	1581, 1585
EcoT38I	GRGCYC	1 cut(s)	214
ErhI	CCWWGG	1 cut(s)	592
Esp3I	CGTCTC	1 cut(s)	1865
FalI	AAGNNNNNCTT	6 cut(s)	973, 1005, 2634, 2666, 2726, 2758
FaqI	GGGAC	4 cut(s)	920, 1030, 1733, 2424
FauNDI	CATATG	1 cut(s)	652
FbaI	TGATCA	1 cut(s)	1998
FblI	GTMKAC	1 cut(s)	1314
FokI	GGATG	6 cut(s)	1270, 1659, 1683, 2096, 2105, 2219
FriOI	GRGCYC	1 cut(s)	214
GlaI	GCGC	2 cut(s)	644, 1346
GsaI	CCCAGC	2 cut(s)	217, 1593
GsuI	CTGGAG	1 cut(s)	1788
HaeII	RGCGCY	2 cut(s)	646, 1348
HaeIII	GGCC	4 cut(s)	120, 920, 2711, 2807
HgaI	GACGC	2 cut(s)	1508, 1847
HhaI	GCGC	2 cut(s)	645, 1347
Hin1I	GRCGYC	1 cut(s)	1858
Hin6I	GCGC	2 cut(s)	643, 1345
HinP1I	GCGC	2 cut(s)	643, 1345
HincII	GTYRAC	2 cut(s)	973, 1381
HindII	GTYRAC	2 cut(s)	973, 1381
HindIII	AAGCTT	2 cut(s)	1161, 2740
HinfI	GANTC	3 cut(s)	656, 1505, 2503
HpaI	GTTAAC	1 cut(s)	973
Hpy166II	GTNNAC	9 cut(s)	23, 97, 973, 1233, 1315, 1381, 1922, 2215, 2245
Hpy8I	GTNNAC	9 cut(s)	23, 97, 973, 1233, 1315, 1381, 1922, 2215, 2245
HpyCH4III	ACNGT	7 cut(s)	13, 101, 321, 583, 1187, 1237, 2600
HpyCH4IV	ACGT	3 cut(s)	141, 667, 2314
HpyF3I	CTNAG	8 cut(s)	180, 1089, 1398, 2202, 2370, 2400, 2733, 2802
HpySE526I	ACGT	3 cut(s)	141, 667, 2314
Hsp92I	GRCGYC	1 cut(s)	1858
HspAI	GCGC	2 cut(s)	643, 1345
Kpn2I	TCCGGA	1 cut(s)	2430
KpnI	GGTACC	1 cut(s)	2846
KroI	GCCGGC	1 cut(s)	116
KroNI	GCCGGC	1 cut(s)	118
Ksp22I	TGATCA	1 cut(s)	1998
KspAI	GTTAAC	1 cut(s)	973
LmnI	GCTCC	8 cut(s)	110, 209, 497, 640, 933, 1171, 1352, 1463
LweI	GCATC	7 cut(s)	139, 369, 945, 1463, 1592, 1661, 1885
MaeII	ACGT	3 cut(s)	141, 667, 2314
MaeIII	GTNAC	6 cut(s)	225, 315, 509, 2104, 2153, 2419
MflI	RGATCY	5 cut(s)	856, 1408, 1593, 2038, 2060
MhlI	GDGCHC	3 cut(s)	214, 1924, 1953
MlsI	TGGCCA	1 cut(s)	2711
MluCI	AATT	9 cut(s)	702, 845, 898, 1147, 1191, 1977, 2076, 2231, 2628
MluNI	TGGCCA	1 cut(s)	2711
MlyI	GAGTC	1 cut(s)	650
MmeI	TCCRAC	6 cut(s)	275, 711, 1436, 2371, 2467, 2731
Mox20I	TGGCCA	1 cut(s)	2711
Mph1103I	ATGCAT	2 cut(s)	1581, 1585
MroI	TCCGGA	1 cut(s)	2430
MroNI	GCCGGC	1 cut(s)	116
MroXI	GAANNNNTTC	2 cut(s)	1009, 2450
MscI	TGGCCA	1 cut(s)	2711
MslI	CAYNNNNRTG	3 cut(s)	2303, 2582, 2705
Msp20I	TGGCCA	1 cut(s)	2711
MspA1I	CMGCKG	1 cut(s)	1631
MspR9I	CCNGG	8 cut(s)	127, 344, 381, 567, 1461, 1559, 2264, 2351
Mva1269I	GAATGC	2 cut(s)	1581, 2646
MvaI	CCWGG	5 cut(s)	344, 381, 567, 1461, 2351
NaeI	GCCGGC	1 cut(s)	118
NciI	CCSGG	3 cut(s)	127, 1559, 2264
NdeI	CATATG	1 cut(s)	652
NgoMIV	GCCGGC	1 cut(s)	116
NheI	GCTAGC	2 cut(s)	356, 2857
NmuCI	GTSAC	5 cut(s)	225, 315, 509, 2153, 2419
NsiI	ATGCAT	2 cut(s)	1581, 1585
NspI	RCATGY	7 cut(s)	408, 682, 1156, 1379, 1517, 1583, 1957
PaeI	GCATGC	4 cut(s)	408, 1156, 1517, 1583
PagI	TCATGA	1 cut(s)	2134
PciI	ACATGT	3 cut(s)	678, 1375, 1953
PcsI	WCGNNNNNNNCGW	1 cut(s)	369
PctI	GAATGC	2 cut(s)	1581, 2646
PdiI	GCCGGC	1 cut(s)	118
PdmI	GAANNNNTTC	2 cut(s)	1009, 2450
PfeI	GAWTC	2 cut(s)	1505, 2503
PflFI	GACNNNGTC	1 cut(s)	581
PflMI	CCANNNNNTGG	2 cut(s)	1049, 2255
PinAI	ACCGGT	1 cut(s)	1766
Ple19I	CGATCG	1 cut(s)	76
PleI	GAGTC	1 cut(s)	650
PmaCI	CACGTG	1 cut(s)	2315
PmlI	CACGTG	1 cut(s)	2315
PpsI	GAGTC	1 cut(s)	650
Ppu21I	YACGTR	1 cut(s)	2315
PscI	ACATGT	3 cut(s)	678, 1375, 1953
Psp6I	CCWGG	5 cut(s)	342, 379, 565, 1459, 2349
PspCI	CACGTG	1 cut(s)	2315
PspEI	GGTNACC	3 cut(s)	509, 2104, 2153
PspFI	CCCAGC	2 cut(s)	213, 1589
PspGI	CCWGG	5 cut(s)	342, 379, 565, 1459, 2349
PspPI	GGNCC	2 cut(s)	238, 918
PstI	CTGCAG	5 cut(s)	198, 970, 2188, 2273, 2335
PstNI	CAGNNNCTG	3 cut(s)	785, 2192, 2407
PsuI	RGATCY	5 cut(s)	856, 1408, 1593, 2038, 2060
PsyI	GACNNNGTC	1 cut(s)	581
PvuI	CGATCG	1 cut(s)	76
PvuII	CAGCTG	1 cut(s)	1631
RsaI	GTAC	9 cut(s)	247, 1273, 1285, 1434, 2288, 2467, 2597, 2673, 2844
RsaNI	GTAC	9 cut(s)	246, 1272, 1284, 1433, 2287, 2466, 2596, 2672, 2843
RseI	CAYNNNNRTG	3 cut(s)	2303, 2582, 2705
Sau96I	GGNCC	2 cut(s)	238, 918
ScaI	AGTACT	2 cut(s)	247, 1434
SchI	GAGTC	1 cut(s)	650
ScrFI	CCNGG	8 cut(s)	127, 344, 381, 567, 1461, 1559, 2264, 2351
SduI	GDGCHC	3 cut(s)	214, 1924, 1953
SfaNI	GCATC	7 cut(s)	139, 369, 945, 1463, 1592, 1661, 1885
SfcI	CTRYAG	6 cut(s)	194, 966, 1311, 2184, 2269, 2331
SgrAI	CRCCGGYG	1 cut(s)	1766
SinI	GGWCC	1 cut(s)	238
SmiMI	CAYNNNNRTG	3 cut(s)	2303, 2582, 2705
SmlI	CTYRAG	6 cut(s)	28, 1862, 2015, 2218, 2473, 2868
SmoI	CTYRAG	6 cut(s)	28, 1862, 2015, 2218, 2473, 2868
SphI	GCATGC	4 cut(s)	408, 1156, 1517, 1583
Sse9I	AATT	9 cut(s)	702, 845, 898, 1147, 1191, 1977, 2076, 2231, 2628
SsiI	CCGC	3 cut(s)	1350, 1815, 2760
StyD4I	CCNGG	8 cut(s)	125, 342, 379, 565, 1459, 1557, 2262, 2349
StyI	CCWWGG	1 cut(s)	592
TaaI	ACNGT	7 cut(s)	13, 101, 321, 583, 1187, 1237, 2600
TaiI	ACGT	3 cut(s)	144, 670, 2317
TasI	AATT	9 cut(s)	702, 845, 898, 1147, 1191, 1977, 2076, 2231, 2628
TatI	WGTACW	2 cut(s)	245, 1432
TfiI	GAWTC	2 cut(s)	1505, 2503
TscAI	CASTG	7 cut(s)	18, 324, 586, 1054, 1240, 1447, 1495
TseFI	GTSAC	5 cut(s)	225, 315, 509, 2153, 2419
Tsp45I	GTSAC	5 cut(s)	225, 315, 509, 2153, 2419
TspDTI	ATGAA	5 cut(s)	326, 1157, 1341, 2123, 2814
TspGWI	ACGGA	3 cut(s)	361, 1562, 2072
TspRI	CASTG	7 cut(s)	18, 324, 586, 1054, 1240, 1447, 1495
Tth111I	GACNNNGTC	1 cut(s)	581
Van91I	CCANNNNNTGG	2 cut(s)	1049, 2255
VneI	GTGCAC	1 cut(s)	1920
VpaK11BI	GGWCC	1 cut(s)	238
XapI	RAATTY	4 cut(s)	702, 845, 1191, 2076
XbaI	TCTAGA	1 cut(s)	1195
XceI	RCATGY	7 cut(s)	408, 682, 1156, 1379, 1517, 1583, 1957
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2588
XmiI	GTMKAC	1 cut(s)	1314
XmnI	GAANNNNTTC	2 cut(s)	1009, 2450
ZrmI	AGTACT	2 cut(s)	247, 1434
Zsp2I	ATGCAT	2 cut(s)	1581, 1585

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.