FvH4_4g16670

Pyruvate phosphate dikinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004385
Physical Location & Seq
Forward (+)
20537404 .. 20544814
7411 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g16670.t1

Sequence Viewer

Length: 2901 bp
ATGTCGTCCTCAGTGAAAGGAATACTTTTGAGGACTTCACCGGAAGTAGCTTATAGACACAGAATGACGTTCAAGGATCGTTCTTATTCCCCCAACGGCGTCAACGGCGGGTCAGCTCGAGCCGGGGGCCAGACGCGGTATCAGCCCCAAGTTTGTACCATAACAAATGGGATCACCAACCCCAGTCCTAAGAAAAATGAGCTCCAGAGCCAGATCGGAGCACAGCCTATTCTGTCACCAGTTTTGGACCCAAGTTCTCCAACAACTCATAAGAGGGTTTTTACTTTTGGGAAAGGAAGGAGTGAAGGAAACAAGGGCATGAAATCACTGTTAGGAGGGAAGGGTGCAAACCTGGCTGAAATGGCTAGCATTGGGCTGTCTGTGCCGCCTGGGCTTACTATCTCAACAGAAGCATGCCAAGAGTATCAGGACAATGGTAAGGATTTACCGGAAGGTTTGTGGGAGGAGATACTAGAAGGCTTGGATTATGTGCAGAAGACTATGGGAGCTACTCTTGGTGACCCTTCCAAGCCCCTCCTTGTCTCCGTTCGCTCTGGTGCTGCAATTTCTATGCCTGGCATGATGGACACTGTCCTCAACCTTGGACTCAATGACGAGGTGGTTACTGGTTTAGCTGCAAAAAGTGGGGAGCGGTTTGCGTATGACTCGTACAGACGTTTCCTAGACATGTTTGGAGATGTTGTTATGGAAATTCCACACTCAAAATTTGAGGAGAAGTTGGAGAACTACAAACACCAAAAAGGAGTTGAGCTTGATACAGAGCTTACAGCGGCTGATCTCAAAGAGTTGGTTGAACAATACAAAAGTGTATATGTTGAAGTTACTGGTAAAAAATTCCCATCAGATCCAAAAGAACAGCTAGTGTTGGCTATTAAAGCAGTTTTTAATTCTTGGGACAGCCCGAGGGCCAACAAATACCGGAGCATCAATCAGATAACTGGTTTAAAGGGAACTGCAGTTAACATTCAATCCATGGCTTATGGGAACATGGGAAATACTTCCGGTACAGGTGTTCTATTCACTAGAAATCCAAGCACTGGTGAAAAGAAGCTCTATGGGGAGTTTCTAATCAATGCTCAGGGAGAAGATGTAGTTGCCGGTATTAGGACACCAGAACCTTTGGATACCATGAAAAATTGCATGCCAGAAGCGTACAAGGAGCTTGTGGAGAACTGTGAAATTCTAGAAAGACATTACAAAGATATGATGGACATTGAGTTCACTGTCCAAGAAAATAGATTATGGATGTTGCAATGCCGTGCTGGAAAGCGTACTGGTAAAGGTGCGTTCAAGATAGCTGTAGACATGGTTAATGAAGGGCTTGTTGATAAGCGCTCTGCAATCAAAATGGTGGAACCACAACATCTTGACCAACTTCTTCACCCTCAGTTTGAGGATCCGACTGCATACAAAGACAAAGTGCTTGCGATTGGCTTGCCTGCATCACCAGGAGCAGCAGTAGGGCAAGTCGTGTTCAGTGCAGAGGATGCTGAAGCATGGCATGCACAAGGAAAGAAAGCCATCTTGGTAAGGACTGAGACCAGTCCGGAAGATATTGGGGGAATGCATGCATCTGCTGGGATCTTGACTGCTAGAGGTGGCATGACATCTCATGCAGCAGTTGTGGCTCGTGGATGGGGTAAATGTTGTGTTTCTGGATGTTCTGATATCCAAGTAAATGATGATGAGAAGGTTGTTGTGATTGGGAGCACCGTGTTTAGAGAAGGAGACTGGCTTTCCCTCAATGGAAACACCGGTGAAGTCATTATTGGAAAACAACCACTTTCTCCTCCAGCTTTAAGCGGCGATTTAGAAACATTTATGTCTTGGGCTGATAGCTTTAGGCGTCTCAAGGTTATGGCAAATGCCGACACACCTGAAGATGCATTAACAGCAAGAAACAATGGTGCACAAGGGATTGGACTTTGTAGGACAGAGCACATGTTCTTTGCTTCAGATGATAGAATTAAGTCAGTAAGAAAGATGATCATGGCAAGTACAGTTGAGCAGAGGCAGGCAGCATTAGACCTCTTACTACCATATCAGAGATCTGACTTTGAAGGAATTTTCCGTGCAATGGATGGTCTTCCAGTAACCATCCGATTGTTAGACCCTCCACTTCATGAGTTTCTTCCAGAAGGTGACCTAGAACAGATTGTTACAGAAGTAGCTGCAGAGACTGGTATGACTGAGGATGCAGTTTACTCAAGAGTTGATAAATTGACAGAAGTAAACCCAATGCTTGGTTTCCGAGGCTGCAGGCTGGGGATATCATACCCAGAACTCACAGAGATGCAAGCGCGTGCAGTGTTTCAGGCTGCAGTCTTAATGAGCAACCAGGGTGTTAAAGTGTTCCCTGAGATAATGGTTCCTCTTGTTGGAACACCTCAGGAACTGGGACACCAAGTGACTTTAATCCGCAGTGTTGCGAAGAAAGTGTTCTCTGAGATGGGTACCACCTTGAGTTTTAAGGTTGGGACCATGATTGAGATTCCTAGAGCTGCTCTAGTTGCAGATGAGATTGCAAAAGAAGCAGACTTCTTTTCTTTCGGAACAAATGATCTCACTCAGATGACCTTTGGGTACAGTAGAGATGATGTGGGCAAGTTCCTAAATACTTATTTGTCCCAAGGCATTCTTCAAAACGATCCATTTGAGGTACTTGATCAAAGAGGAGTTGGGCAACTCGTCAAGATGGCCACAGAAAAGGGTCGTGCAGCTAAGCCTAGCTTAAAGGTTGGAATATGTGGAGAGCATGGTGGGGAGCCTTCTTCAGTTGCATTTTTCGCAGAGGCTGGATTAGACTATGTTTCATGTTCTCCATTCAGGATACCAATTGCAAGGCTAGCAGCAGCTCAAGTTGCCGTGACAAAGAGTTAA

Protein Analysis

967

Amino Acids

105.13

Weight (kDa)

5.59

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPDK_N PF01326 109 - 145 1.2e-06 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N PF01326 148 - 380 8.7e-23 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N PF01326 391 - 445 6.8e-10 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PEP-utilizers PF00391 512 - 593 1.1e-22 PEP-utilising enzyme, mobile domain
PEP-utilizers_C PF02896 608 - 962 1.9e-92 PEP-utilising enzyme, PEP-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2474
AccB1I GGYRCC 1 cut(s) 2474
AccB7I CCANNNNNTGG 2 cut(s) 1058, 2264
AccBSI CCGCTC 1 cut(s) 652
AccI GTMKAC 1 cut(s) 1323
AccII CGCG 2 cut(s) 136, 2323
AccIII TCCGGA 1 cut(s) 1567
AciI CCGC 7 cut(s) 108, 136, 386, 652, 791, 1824, 2440
AclWI GGATC 7 cut(s) 84, 179, 860, 1412, 1425, 1610, 2663
AcoI YGGCCR 1 cut(s) 2718
AcsI RAATTY 5 cut(s) 711, 725, 854, 1200, 2085
AcuI CTGAAG 4 cut(s) 1533, 1920, 1959, 2778
AcyI GRCGYC 2 cut(s) 99, 1867
AdeI CACNNNGTG 1 cut(s) 2428
AfaI GTAC 9 cut(s) 157, 671, 1027, 1175, 1294, 2020, 2476, 2606, 2682
AfeI AGCGCT 1 cut(s) 1355
AfiI CCNNNNNNNGG 5 cut(s) 1058, 1125, 2098, 2264, 2399
AflIII ACRYGT 2 cut(s) 687, 1962
AgeI ACCGGT 1 cut(s) 1775
AgsI TTSAA 7 cut(s) 73, 815, 839, 989, 1312, 2081, 2663
AjnI CCWGG 5 cut(s) 351, 388, 574, 1468, 2358
AjuI GAANNNNNNNTTGG 6 cut(s) 1529, 1561, 1773, 1805, 2643, 2675
AloI GAACNNNNNNTCC 2 cut(s) 1223, 1255
Alw21I GWGCWC 5 cut(s) 204, 223, 1733, 1933, 1962
Alw26I GTCTC 5 cut(s) 547, 1553, 1743, 1874, 2192
Alw44I GTGCAC 1 cut(s) 1929
AlwI GGATC 7 cut(s) 84, 179, 860, 1412, 1425, 1610, 2663
AlwNI CAGNNNCTG 4 cut(s) 794, 2201, 2416, 2816
Ama87I CYCGRG 2 cut(s) 117, 922
Aor13HI TCCGGA 1 cut(s) 1567
Aor51HI AGCGCT 1 cut(s) 1355
AoxI GGCC 3 cut(s) 127, 927, 2718
ApaLI GTGCAC 1 cut(s) 1929
ApoI RAATTY 5 cut(s) 711, 725, 854, 1200, 2085
AsiGI ACCGGT 1 cut(s) 1775
Asp700I GAANNNNTTC 2 cut(s) 1018, 2459
Asp718I GGTACC 1 cut(s) 2474
AspLEI GCGC 2 cut(s) 1356, 2323
AspS9I GGNCC 4 cut(s) 127, 247, 927, 2499
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 9 cut(s) 30, 166, 228, 530, 1073, 1394, 1458, 1790, 2174
AsuNHI GCTAGC 2 cut(s) 365, 2866
AvaI CYCGRG 2 cut(s) 117, 922
AvaII GGWCC 2 cut(s) 247, 2499
AxyI CCTNAGG 1 cut(s) 2409
BaeGI GKGCMC 1 cut(s) 1933
BaeI ACNNNNGTAYC 2 cut(s) 1017, 1050
BalI TGGCCA 1 cut(s) 2720
BamHI GGATCC 1 cut(s) 1417
BanI GGYRCC 1 cut(s) 2474
BanII GRGCYC 1 cut(s) 204
BauI CACGAG 1 cut(s) 1650
BbsI GAAGAC 2 cut(s) 503, 2099
Bbv12I GWGCWC 5 cut(s) 204, 223, 1733, 1933, 1962
BccI CCATC 9 cut(s) 577, 868, 1222, 1550, 1650, 2096, 2126, 2464, 2710
BceAI ACGGC 4 cut(s) 112, 121, 1263, 2870
BcgI CGANNNNNNTGC 2 cut(s) 1438, 1472
BciT130I CCWGG 5 cut(s) 353, 390, 576, 1470, 2360
BciVI GTATCC 2 cut(s) 1138, 2844
BclI TGATCA 2 cut(s) 2007, 2686
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 5 cut(s) 547, 1553, 1743, 1874, 2192
BfmI CTRYAG 5 cut(s) 975, 1320, 2193, 2278, 2340
BfoI RGCGCY 1 cut(s) 1357
BfuI GTATCC 2 cut(s) 1138, 2844
BglI GCCNNNNNGGC 1 cut(s) 391
BglII AGATCT 1 cut(s) 2069
BlpI GCTNAGC 1 cut(s) 2742
Bme1390I CCNGG 6 cut(s) 124, 353, 390, 576, 1470, 2360
Bme18I GGWCC 2 cut(s) 247, 2499
BmeT110I CYCGRG 2 cut(s) 117, 922
BmgT120I GGNCC 4 cut(s) 127, 247, 927, 2499
BmiI GGNNCC 8 cut(s) 128, 249, 1377, 1419, 2391, 2476, 2500, 2787
BmrFI CCNGG 6 cut(s) 124, 353, 390, 576, 1470, 2360
BmrI ACTGGG 2 cut(s) 177, 2426
BmsI GCATC 7 cut(s) 954, 1472, 1498, 1601, 1894, 2206, 2304
BmtI GCTAGC 2 cut(s) 369, 2870
BmuI ACTGGG 2 cut(s) 177, 2426
BpiI GAAGAC 2 cut(s) 503, 2099
BpmI CTGGAG 2 cut(s) 188, 1797
Bpu10I CCTNAGC 1 cut(s) 1098
Bpu1102I GCTNAGC 1 cut(s) 2742
BpuEI CTTGAG 4 cut(s) 1856, 2212, 2503, 2862
BpuMI CCSGG 1 cut(s) 124
BsaHI GRCGYC 2 cut(s) 99, 1867
BsaI GGTCTC 1 cut(s) 1553
BsaJI CCNNGG 8 cut(s) 123, 389, 601, 923, 993, 2272, 2359, 2650
BsaWI WCCGGW 6 cut(s) 40, 448, 939, 1022, 1567, 1775
BsaXI ACNNNNNCTCC 6 cut(s) 687, 717, 1095, 1125, 1464, 1494
Bsc4I CCNNNNNNNGG 5 cut(s) 1058, 1125, 2098, 2264, 2399
Bse118I RCCGGY 2 cut(s) 1118, 1775
Bse21I CCTNAGG 1 cut(s) 2409
Bse3DI GCAATG 2 cut(s) 1280, 2103
BseAI TCCGGA 1 cut(s) 1567
BseBI CCWGG 5 cut(s) 353, 390, 576, 1470, 2360
BseDI CCNNGG 8 cut(s) 123, 389, 601, 923, 993, 2272, 2359, 2650
BseGI GGATG 7 cut(s) 1272, 1513, 1661, 1685, 2107, 2118, 2221
BseLI CCNNNNNNNGG 5 cut(s) 1058, 1125, 2098, 2264, 2399
BseMI GCAATG 2 cut(s) 1280, 2103
BseMII CTCAG 9 cut(s) 24, 1112, 1421, 1548, 2202, 2370, 2423, 2457, 2603
BseRI GAGGAG 4 cut(s) 479, 746, 1800, 2709
BseSI GKGCMC 1 cut(s) 1933
BseYI CCCAGC 2 cut(s) 1598, 2284
BsgI GTGCAG 4 cut(s) 512, 1521, 2346, 2757
Bsh1236I CGCG 2 cut(s) 136, 2323
BshFI GGCC 3 cut(s) 129, 929, 2720
BshNI GGYRCC 1 cut(s) 2474
BshTI ACCGGT 1 cut(s) 1775
BsiHKAI GWGCWC 5 cut(s) 204, 223, 1733, 1933, 1962
BsiHKCI CYCGRG 2 cut(s) 117, 922
BsiSI CCGG 8 cut(s) 41, 123, 449, 940, 1023, 1119, 1568, 1776
BslFI GGGAC 4 cut(s) 929, 2433, 2512, 2632
BslI CCNNNNNNNGG 5 cut(s) 1058, 1125, 2098, 2264, 2399
BsmAI GTCTC 5 cut(s) 547, 1553, 1743, 1874, 2192
BsmBI CGTCTC 1 cut(s) 1874
BsmFI GGGAC 4 cut(s) 929, 2433, 2512, 2632
BsmI GAATGC 2 cut(s) 1590, 2655
BsnI GGCC 3 cut(s) 129, 929, 2720
Bso31I GGTCTC 1 cut(s) 1553
BsoBI CYCGRG 2 cut(s) 117, 922
Bsp1286I GDGCHC 5 cut(s) 204, 223, 1733, 1933, 1962
Bsp13I TCCGGA 1 cut(s) 1567
Bsp1720I GCTNAGC 1 cut(s) 2742
Bsp19I CCATGG 1 cut(s) 993
BspACI CCGC 7 cut(s) 108, 136, 386, 652, 791, 1824, 2440
BspANI GGCC 3 cut(s) 129, 929, 2720
BspCNI CTCAG 9 cut(s) 23, 1111, 1420, 1549, 2203, 2371, 2422, 2458, 2602
BspEI TCCGGA 1 cut(s) 1567
BspFNI CGCG 2 cut(s) 136, 2323
BspHI TCATGA 1 cut(s) 2143
BspLI GGNNCC 8 cut(s) 128, 249, 1377, 1419, 2391, 2476, 2500, 2787
BspMAI CTGCAG 4 cut(s) 979, 2197, 2282, 2344
BspOI GCTAGC 2 cut(s) 369, 2870
BspPI GGATC 7 cut(s) 84, 179, 860, 1412, 1425, 1610, 2663
BspT107I GGYRCC 1 cut(s) 2474
BspTNI GGTCTC 1 cut(s) 1553
BsrBI CCGCTC 1 cut(s) 652
BsrDI GCAATG 2 cut(s) 1280, 2103
BsrFI RCCGGY 2 cut(s) 1118, 1775
BssAI RCCGGY 2 cut(s) 1118, 1775
BssECI CCNNGG 8 cut(s) 123, 389, 601, 923, 993, 2272, 2359, 2650
BssNI GRCGYC 2 cut(s) 99, 1867
BssSI CACGAG 1 cut(s) 1650
BssT1I CCWWGG 3 cut(s) 601, 993, 2650
Bst2BI CACGAG 1 cut(s) 1650
Bst2UI CCWGG 5 cut(s) 353, 390, 576, 1470, 2360
Bst4CI ACNGT 7 cut(s) 330, 592, 1196, 1246, 1735, 2023, 2609
BstACI GRCGYC 2 cut(s) 99, 1867
BstAPI GCANNNNNTGC 2 cut(s) 1508, 1523
BstDSI CCRYGG 1 cut(s) 993
BstEII GGTNACC 2 cut(s) 518, 2162
BstF5I GGATG 7 cut(s) 1272, 1513, 1661, 1685, 2107, 2118, 2221
BstFNI CGCG 2 cut(s) 136, 2323
BstH2I RGCGCY 1 cut(s) 1357
BstHHI GCGC 2 cut(s) 1356, 2323
BstMAI GTCTC 5 cut(s) 547, 1553, 1743, 1874, 2192
BstNI CCWGG 5 cut(s) 353, 390, 576, 1470, 2360
BstNSI RCATGY 6 cut(s) 417, 691, 1165, 1526, 1592, 1966
BstPI GGTNACC 2 cut(s) 518, 2162
BstSCI CCNGG 6 cut(s) 122, 351, 388, 574, 1468, 2358
BstSFI CTRYAG 5 cut(s) 975, 1320, 2193, 2278, 2340
BstSLI GKGCMC 1 cut(s) 1933
BstUI CGCG 2 cut(s) 136, 2323
BstV2I GAAGAC 2 cut(s) 503, 2099
BstX2I RGATCY 4 cut(s) 865, 1417, 1602, 2069
BstYI RGATCY 4 cut(s) 865, 1417, 1602, 2069
Bsu36I CCTNAGG 1 cut(s) 2409
BsuI GTATCC 2 cut(s) 1138, 2844
BsuRI GGCC 3 cut(s) 129, 929, 2720
BtgI CCRYGG 1 cut(s) 993
BtsCI GGATG 7 cut(s) 1272, 1513, 1661, 1685, 2107, 2118, 2221
BtsI GCAGTG 2 cut(s) 2334, 2449
BtsIMutI CAGTG 8 cut(s) 18, 326, 588, 1056, 1242, 1504, 2334, 2449
CaiI CAGNNNCTG 4 cut(s) 794, 2201, 2416, 2816
CciI TCATGA 1 cut(s) 2143
CfoI GCGC 2 cut(s) 1356, 2323
Cfr10I RCCGGY 2 cut(s) 1118, 1775
Cfr13I GGNCC 4 cut(s) 127, 247, 927, 2499
CseI GACGC 3 cut(s) 88, 142, 1856
Csp6I GTAC 9 cut(s) 156, 670, 1026, 1174, 1293, 2019, 2475, 2605, 2681
CspAI ACCGGT 1 cut(s) 1775
CviQI GTAC 9 cut(s) 156, 670, 1026, 1174, 1293, 2019, 2475, 2605, 2681
DraI TTTAAA 1 cut(s) 966
DraIII CACNNNGTG 1 cut(s) 2428
EaeI YGGCCR 1 cut(s) 2718
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 3 cut(s) 601, 993, 2650
Eco24I GRGCYC 1 cut(s) 204
Eco31I GGTCTC 1 cut(s) 1553
Eco32I GATATC 2 cut(s) 1690, 2292
Eco47I GGWCC 2 cut(s) 247, 2499
Eco47III AGCGCT 1 cut(s) 1355
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 4 cut(s) 1533, 1920, 1959, 2778
Eco81I CCTNAGG 1 cut(s) 2409
Eco88I CYCGRG 2 cut(s) 117, 922
Eco91I GGTNACC 2 cut(s) 518, 2162
EcoICRI GAGCTC 1 cut(s) 202
EcoO65I GGTNACC 2 cut(s) 518, 2162
EcoRII CCWGG 5 cut(s) 351, 388, 574, 1468, 2358
EcoRV GATATC 2 cut(s) 1690, 2292
EcoT14I CCWWGG 3 cut(s) 601, 993, 2650
EcoT22I ATGCAT 3 cut(s) 1590, 1594, 1909
EcoT38I GRGCYC 1 cut(s) 204
ErhI CCWWGG 3 cut(s) 601, 993, 2650
Esp3I CGTCTC 1 cut(s) 1874
FalI AAGNNNNNCTT 6 cut(s) 9, 41, 2643, 2675, 2735, 2767
FaqI GGGAC 4 cut(s) 929, 2433, 2512, 2632
FauI CCCGC 1 cut(s) 101
FbaI TGATCA 2 cut(s) 2007, 2686
FblI GTMKAC 1 cut(s) 1323
FokI GGATG 7 cut(s) 1279, 1520, 1668, 1692, 2105, 2114, 2228
FriOI GRGCYC 1 cut(s) 204
GlaI GCGC 2 cut(s) 1355, 2322
GsaI CCCAGC 2 cut(s) 1602, 2288
GsuI CTGGAG 2 cut(s) 188, 1797
HaeII RGCGCY 1 cut(s) 1357
HaeIII GGCC 3 cut(s) 129, 929, 2720
HapII CCGG 8 cut(s) 41, 123, 449, 940, 1023, 1119, 1568, 1776
HgaI GACGC 3 cut(s) 88, 142, 1856
HhaI GCGC 2 cut(s) 1356, 2323
Hin1I GRCGYC 2 cut(s) 99, 1867
Hin6I GCGC 2 cut(s) 1354, 2321
HinP1I GCGC 2 cut(s) 1354, 2321
HincII GTYRAC 2 cut(s) 103, 982
HindII GTYRAC 2 cut(s) 103, 982
HinfI GANTC 3 cut(s) 606, 665, 2512
HpaI GTTAAC 1 cut(s) 982
HpaII CCGG 8 cut(s) 41, 123, 449, 940, 1023, 1119, 1568, 1776
HphI GGTGA 9 cut(s) 30, 166, 228, 530, 1073, 1394, 1458, 1790, 2174
Hpy166II GTNNAC 7 cut(s) 103, 982, 1242, 1324, 1931, 2224, 2254
Hpy8I GTNNAC 7 cut(s) 103, 982, 1242, 1324, 1931, 2224, 2254
HpyCH4III ACNGT 7 cut(s) 330, 592, 1196, 1246, 1735, 2023, 2609
HpyCH4IV ACGT 2 cut(s) 68, 676
HpySE526I ACGT 2 cut(s) 68, 676
Hsp92I GRCGYC 2 cut(s) 99, 1867
HspAI GCGC 2 cut(s) 1354, 2321
Kpn2I TCCGGA 1 cut(s) 1567
KpnI GGTACC 1 cut(s) 2478
Ksp22I TGATCA 2 cut(s) 2007, 2686
KspAI GTTAAC 1 cut(s) 982
LmnI GCTCC 9 cut(s) 207, 218, 506, 649, 942, 1180, 1472, 1728, 2785
LweI GCATC 7 cut(s) 954, 1472, 1498, 1601, 1894, 2206, 2304
MaeII ACGT 2 cut(s) 68, 676
MaeIII GTNAC 9 cut(s) 234, 518, 622, 841, 2113, 2162, 2179, 2428, 2887
MbiI CCGCTC 1 cut(s) 652
MfeI CAATTG 1 cut(s) 2856
MflI RGATCY 4 cut(s) 865, 1417, 1602, 2069
MhlI GDGCHC 5 cut(s) 204, 223, 1733, 1933, 1962
MlsI TGGCCA 1 cut(s) 2720
MluNI TGGCCA 1 cut(s) 2720
MlyI GAGTC 2 cut(s) 600, 659
MmeI TCCRAC 5 cut(s) 284, 720, 1445, 2380, 2740
Mox20I TGGCCA 1 cut(s) 2720
Mph1103I ATGCAT 3 cut(s) 1590, 1594, 1909
MroI TCCGGA 1 cut(s) 1567
MroXI GAANNNNTTC 2 cut(s) 1018, 2459
MscI TGGCCA 1 cut(s) 2720
MslI CAYNNNNRTG 2 cut(s) 2312, 2591
Msp20I TGGCCA 1 cut(s) 2720
MspA1I CMGCKG 1 cut(s) 791
MspI CCGG 8 cut(s) 41, 123, 449, 940, 1023, 1119, 1568, 1776
MspR9I CCNGG 6 cut(s) 124, 353, 390, 576, 1470, 2360
MunI CAATTG 1 cut(s) 2856
Mva1269I GAATGC 2 cut(s) 1590, 2655
MvaI CCWGG 5 cut(s) 353, 390, 576, 1470, 2360
MvnI CGCG 2 cut(s) 136, 2323
NciI CCSGG 1 cut(s) 124
NcoI CCATGG 1 cut(s) 993
NheI GCTAGC 2 cut(s) 365, 2866
NlaIV GGNNCC 8 cut(s) 128, 249, 1377, 1419, 2391, 2476, 2500, 2787
NmuCI GTSAC 5 cut(s) 234, 518, 2162, 2428, 2887
NsiI ATGCAT 3 cut(s) 1590, 1594, 1909
NspI RCATGY 6 cut(s) 417, 691, 1165, 1526, 1592, 1966
PaeI GCATGC 4 cut(s) 417, 1165, 1526, 1592
PaeR7I CTCGAG 1 cut(s) 117
PagI TCATGA 1 cut(s) 2143
PciI ACATGT 2 cut(s) 687, 1962
PctI GAATGC 2 cut(s) 1590, 2655
PdmI GAANNNNTTC 2 cut(s) 1018, 2459
PfeI GAWTC 1 cut(s) 2512
PflFI GACNNNGTC 1 cut(s) 590
PflMI CCANNNNNTGG 2 cut(s) 1058, 2264
PinAI ACCGGT 1 cut(s) 1775
PleI GAGTC 2 cut(s) 600, 659
PpsI GAGTC 2 cut(s) 600, 659
PscI ACATGT 2 cut(s) 687, 1962
Psp124BI GAGCTC 1 cut(s) 204
Psp6I CCWGG 5 cut(s) 351, 388, 574, 1468, 2358
PspEI GGTNACC 2 cut(s) 518, 2162
PspFI CCCAGC 2 cut(s) 1598, 2284
PspGI CCWGG 5 cut(s) 351, 388, 574, 1468, 2358
PspN4I GGNNCC 8 cut(s) 128, 249, 1377, 1419, 2391, 2476, 2500, 2787
PspPI GGNCC 4 cut(s) 127, 247, 927, 2499
PspXI VCTCGAGB 1 cut(s) 117
PstI CTGCAG 4 cut(s) 979, 2197, 2282, 2344
PstNI CAGNNNCTG 4 cut(s) 794, 2201, 2416, 2816
PsuI RGATCY 4 cut(s) 865, 1417, 1602, 2069
PsyI GACNNNGTC 1 cut(s) 590
RsaI GTAC 9 cut(s) 157, 671, 1027, 1175, 1294, 2020, 2476, 2606, 2682
RsaNI GTAC 9 cut(s) 156, 670, 1026, 1174, 1293, 2019, 2475, 2605, 2681
RseI CAYNNNNRTG 2 cut(s) 2312, 2591
SacI GAGCTC 1 cut(s) 204
Sau96I GGNCC 4 cut(s) 127, 247, 927, 2499
SchI GAGTC 2 cut(s) 600, 659
ScrFI CCNGG 6 cut(s) 124, 353, 390, 576, 1470, 2360
SduI GDGCHC 5 cut(s) 204, 223, 1733, 1933, 1962
SfaNI GCATC 7 cut(s) 954, 1472, 1498, 1601, 1894, 2206, 2304
SfcI CTRYAG 5 cut(s) 975, 1320, 2193, 2278, 2340
Sfr274I CTCGAG 1 cut(s) 117
SgrAI CRCCGGYG 1 cut(s) 1775
SinI GGWCC 2 cut(s) 247, 2499
SlaI CTCGAG 1 cut(s) 117
SmiMI CAYNNNNRTG 2 cut(s) 2312, 2591
SmlI CTYRAG 5 cut(s) 117, 1871, 2227, 2482, 2877
SmoI CTYRAG 5 cut(s) 117, 1871, 2227, 2482, 2877
SphI GCATGC 4 cut(s) 417, 1165, 1526, 1592
SsiI CCGC 7 cut(s) 108, 136, 386, 652, 791, 1824, 2440
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 6 cut(s) 122, 351, 388, 574, 1468, 2358
StyI CCWWGG 3 cut(s) 601, 993, 2650
TaaI ACNGT 7 cut(s) 330, 592, 1196, 1246, 1735, 2023, 2609
TaiI ACGT 2 cut(s) 71, 679
TaqI TCGA 1 cut(s) 118
TatI WGTACW 1 cut(s) 2018
TauI GCSGC 3 cut(s) 388, 794, 1827
TfiI GAWTC 1 cut(s) 2512
TscAI CASTG 8 cut(s) 18, 333, 595, 1063, 1249, 1504, 2334, 2449
TseFI GTSAC 5 cut(s) 234, 518, 2162, 2428, 2887
Tsp45I GTSAC 5 cut(s) 234, 518, 2162, 2428, 2887
TspDTI ATGAA 5 cut(s) 335, 1166, 1350, 2132, 2823
TspGWI ACGGA 2 cut(s) 535, 2081
TspRI CASTG 8 cut(s) 18, 333, 595, 1063, 1249, 1504, 2334, 2449
Tth111I GACNNNGTC 1 cut(s) 590
Van91I CCANNNNNTGG 2 cut(s) 1058, 2264
VneI GTGCAC 1 cut(s) 1929
VpaK11BI GGWCC 2 cut(s) 247, 2499
XapI RAATTY 5 cut(s) 711, 725, 854, 1200, 2085
XbaI TCTAGA 1 cut(s) 1204
XceI RCATGY 6 cut(s) 417, 691, 1165, 1526, 1592, 1966
XhoI CTCGAG 1 cut(s) 117
XmiI GTMKAC 1 cut(s) 1323
XmnI GAANNNNTTC 2 cut(s) 1018, 2459
Zsp2I ATGCAT 3 cut(s) 1590, 1594, 1909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.