MD16G1179400.v1.1

Pyruvate phosphate dikinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004385
Physical Location & Seq
Reverse (-)
15369249 .. 15375028
5780 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1179400.v1.1.491

Sequence Viewer

Length: 2592 bp
ATGAAGTCATTGTTGGGAGGGAAAGGTGCAAACCTGGCAGAAATGGCTAGCATTGGGTTATCCGTCCCGCCTGGACTTACTATATCAACAGAAGCATGCCAAGAGTATCAGCTCAATGGCAAAGACTTACCTCTAGGTTTGTGGGAGGAGATATTAGAGGGCTTGGAGATTGTGCAGGAGGACATTGGTGCTGTTCTTGGAGATCCTTCCAAGCCTCTTCTCGTCTCTGTTCGCTCTGGTGCTGCGATTTCCATGCCTGGCATGATGGACACTGTCCTCAACCTTGGGCTCAATGACGAGGTGGTTGCTGGTTTGGCCGCAAAAAGTGGAGAGCGCTTTGCATATGACTCTTACAGACGGTTCCTGGACATGTTTGGTGATGTTGTTATGGGAATTCCACACTCATCATTCGAGGAGAAATTGGAAAAACTAAAACGTAAAAAAAGAGTTGAGCTTGATACAAAGCTAACAGCTTCTGATCTTAAAGAGCTGGTAGAACAATACAAAAATGTTTATCTCGAAACAACGGGTAAAAAGTTCCCTTCAGATCCAAAACAACAGCTTATCTTGGCTGTTAAAGCAGTTTTTGATTCTTGGGACAGCCAAAGGGCCAGTAAATACCGGAGCATCAATCAGATAACTGGTTTGAAGGGAACCGCGGTTAGCATTCAATGCATGGTTTTCGGGAACATGGGAAATACTTCAGGGACAGGTGTTCTGTTCACTAGGAATCCAAGCACCGGTGAAAGGAAGCTTTATGGCGAGTTTCTAGTCAATGCTCAGGGAGAAGATGTAGTTGCCGGGATCAGGACACCAGAAGACGTGGATGCTATGAAAAATTGCATGCCAGAAGCTTACAAGGAGCTTGTGGAGAACTGTGAAATTCTAGAAAAACATTACAAAGATATGATGGACATCGAGTTTACGGTCCAAGAAAATAGGTTGTGGATGTTGCAATGCCGCGTTGGAAAGCGTACTGGAAAAGGAGCGATTAAGATAGCTGTGGACATGGCTAATGAGGGGCTCGTGGATAAGCGCACAGCAATCAAGATGGTGGAACCACAGCACCTTGACCAACTTCTTCACCCACAGTTTGAGAATCCAACCGCTTACAAAAACAAAGTCATCGCCATTGGATTGCCTGCATCTCCAGGAGCAGCTGTAGGGACAGTCGTGTTCAGTGCTGAGGATGCCGAAACATGGCAGGCACAAGGAAAGAGTGTCATCTTGGTAAGGACTGAAACTAGCCCCGAAGATGTTGGGGGAATGCATGCAGCTGCAGGGATCCTGACAGCCAGAGGAGGCATGACATCTCATGCAGCTGTTGTAGCCCGTGGGTGGGGCAAATGTTGTGTTTCTGGCTGCGCTGATATCCGAGTAAATGACGCTGAGAAGCTGGTTGTGATCGGGAACACGGTGGTCAAAGAAGGAGAATGGATTTCGCTCAATGGATCCACTGGTGAAGTCATATTAGGAAAACAGCCACTTTCTCCTCCGGCTTTTAGTGGCGATTTGGAAACCTTTATGTCTTGGGCTGACAATGTCCGGCGTCTCAAGGTTATGGCGAATGCTGACACACCTGAAGACGCACTAACTGCAAGAAATAACGGTGCACAAGGGATTGGACTTTGCAGGACAGAGCACATGTTCTTTGCTTCGGATGATAGAATAAAGGCGGTAAGAAAAATGATCATGGCGGCTACACCTGAGCAGAGGAAGGTGGCATTGGACCTCTTACTGCCGTATCAAAGATCTGACTTTGAAGGAATTTTCCGTGCAATGGATGGTCTTCCGGTTACAATCCGCCTTTTAGACCCTCCACTTCATGAATTTCTTCCAGAAGGTAACATGGAACAGATTGTTGGCCAACTAACTGCAGAGACTGGCATGACTGAGGGAGAAGTTTTTTCAAGAGTTGATAAATTATCAGAAGTAAATCCGATGCTTGGTTTCCGTGGCTGCAGGCTAGGCATATCGTACCCAGAACTCACTGAAATGCAGGCGCGTGCAATCTTTCAGGCTGCAGTCTCAATGAGCAACCAGGGTGTCAAAGTTTTCCCCGAGATAATGGTTCCCCTTGAACTAGGACATCAAGCGAAGGTCATTCACGGTGTTGCAGTTAAAGTGTTCTCCGAGATGGGTACCACCTTGAGCTATAAGGTTGGGACTATGATTGAGATCCCTAGAGCTGCTCTTGTTGCAGATGAGATTGCGAAAGAAGCTGACTTCTTCTCCTTCGGGACCAATGATCTCACCCAAATGACCTTTGGATACAGTAGAGATGATGTGGGCAAGTTTCTACCTATTTACCTGTCCAAAGGCCTTCTTCAAAGCGATCCATTCCAGGTACTTGATCAAGGAGGTGTCGGGCAGCTCATCAAGATGGCCATGGAAAAGGGACGCGCAGCTAGGCCTAGCTTAAAGGTTGGAATATGTGGAGAGCATGGGGGAGAGCCTTCTTCTGTTGCCTTTTTTGCAGAGGCCGGACTAGACTATGTTTCGTGTTCTCCATTCAGGGTACCTATTGCTAGGCTAGCAGCAGCTCAAGTTGCTGTTGAGGAAGGCTGCATAACAGAGAATCTGTGCTTGTAA

Protein Analysis

864

Amino Acids

93.72

Weight (kDa)

5.28

Isoelectric Point (pI)

32.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPDK_N PF01326 3 - 52 8.7e-07 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N PF01326 46 - 276 3.8e-22 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N PF01326 285 - 339 5.7e-10 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PEP-utilizers PF00391 406 - 487 2.4e-23 PEP-utilising enzyme, mobile domain
PEP-utilizers_C PF02896 502 - 851 9.8e-88 PEP-utilising enzyme, PEP-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 2141, 2518
AccB1I GGYRCC 2 cut(s) 2141, 2518
AccII CGCG 4 cut(s) 659, 963, 2005, 2403
AciI CCGC 9 cut(s) 68, 318, 657, 659, 961, 1107, 1676, 1697, 1804
AclWI GGATC 9 cut(s) 197, 542, 812, 1279, 1292, 1446, 1459, 2173, 2330
AcoI YGGCCR 3 cut(s) 315, 1864, 2385
AcsI RAATTY 4 cut(s) 393, 882, 1767, 1829
AcuI CTGAAG 3 cut(s) 528, 687, 1602
AcyI GRCGYC 1 cut(s) 1549
AfaI GTAC 5 cut(s) 976, 1979, 2143, 2349, 2520
AfeI AGCGCT 1 cut(s) 335
AfiI CCNNNNNNNGG 8 cut(s) 740, 747, 807, 1200, 1338, 1339, 1780, 1946
AflIII ACRYGT 2 cut(s) 369, 1644
AgeI ACCGGT 1 cut(s) 740
AgsI TTSAA 6 cut(s) 649, 671, 1763, 1911, 2081, 2330
AjiI CACGTC 1 cut(s) 823
AjnI CCWGG 7 cut(s) 33, 70, 256, 363, 1150, 2040, 2343
AjuI GAANNNNNNNTTGG 5 cut(s) 28, 1709, 1741, 1845, 1877
Alw21I GWGCWC 2 cut(s) 1615, 1644
Alw26I GTCTC 4 cut(s) 229, 1556, 1874, 2032
Alw44I GTGCAC 1 cut(s) 1611
AlwI GGATC 9 cut(s) 197, 542, 812, 1279, 1292, 1446, 1459, 2173, 2330
AlwNI CAGNNNCTG 2 cut(s) 476, 1883
Ama87I CYCGRG 1 cut(s) 2060
Aor51HI AGCGCT 1 cut(s) 335
AoxI GGCC 7 cut(s) 315, 609, 1864, 2320, 2385, 2411, 2481
ApaLI GTGCAC 1 cut(s) 1611
ApoI RAATTY 4 cut(s) 393, 882, 1767, 1829
ArsI GACNNNNNNTTYG 2 cut(s) 1107, 1139
AsiGI ACCGGT 1 cut(s) 740
Asp700I GAANNNNTTC 2 cut(s) 700, 1833
Asp718I GGTACC 2 cut(s) 2141, 2518
AspLEI GCGC 5 cut(s) 336, 1038, 1367, 2005, 2405
AspS9I GGNCC 4 cut(s) 609, 928, 1729, 2241
AsuC2I CCSGG 1 cut(s) 802
AsuHPI GGTGA 5 cut(s) 389, 755, 1076, 1472, 2245
AsuNHI GCTAGC 2 cut(s) 47, 2533
AvaI CYCGRG 1 cut(s) 2060
AvaII GGWCC 3 cut(s) 928, 1729, 2241
BaeGI GKGCMC 1 cut(s) 1615
BaeI ACNNNNGTAYC 2 cut(s) 1727, 1760
BalI TGGCCA 2 cut(s) 1866, 2387
BamHI GGATCC 2 cut(s) 1284, 1451
BanI GGYRCC 2 cut(s) 2141, 2518
BanII GRGCYC 2 cut(s) 291, 1026
BauI CACGAG 1 cut(s) 1025
BbsI GAAGAC 3 cut(s) 825, 1590, 1781
Bbv12I GWGCWC 2 cut(s) 1615, 1644
BbvCI CCTCAGC 1 cut(s) 1185
BccI CCATC 6 cut(s) 259, 904, 1045, 1778, 2131, 2377
BceAI ACGGC 1 cut(s) 1726
BcgI CGANNNNNNTGC 6 cut(s) 235, 269, 287, 321, 664, 698
BciT130I CCWGG 7 cut(s) 35, 72, 258, 365, 1152, 2042, 2345
BciVI GTATCC 1 cut(s) 2264
BclI TGATCA 2 cut(s) 1689, 2353
BcnI CCSGG 1 cut(s) 802
BcoDI GTCTC 4 cut(s) 229, 1556, 1874, 2032
BfmI CTRYAG 5 cut(s) 1161, 1278, 1875, 1960, 2022
BfoI RGCGCY 1 cut(s) 337
BfuI GTATCC 1 cut(s) 2264
BglII AGATCT 1 cut(s) 1751
Bme1390I CCNGG 8 cut(s) 35, 72, 258, 365, 802, 1152, 2042, 2345
Bme18I GGWCC 3 cut(s) 928, 1729, 2241
BmeT110I CYCGRG 1 cut(s) 2060
BmgBI CACGTC 1 cut(s) 823
BmgT120I GGNCC 4 cut(s) 609, 928, 1729, 2241
BmiI GGNNCC 9 cut(s) 362, 655, 1059, 1286, 1453, 2073, 2143, 2242, 2520
BmrFI CCNGG 8 cut(s) 35, 72, 258, 365, 802, 1152, 2042, 2345
BmsI GCATC 5 cut(s) 636, 817, 1154, 1180, 1932
BmtI GCTAGC 2 cut(s) 51, 2537
BpiI GAAGAC 3 cut(s) 825, 1590, 1781
BpmI CTGGAG 1 cut(s) 1134
Bpu10I CCTNAGC 3 cut(s) 780, 1185, 1707
BpuEI CTTGAG 3 cut(s) 1538, 2170, 2529
BpuMI CCSGG 1 cut(s) 802
BsaBI GATNNNNATC 2 cut(s) 914, 2177
BsaHI GRCGYC 1 cut(s) 1549
BsaJI CCNNGG 6 cut(s) 283, 657, 1333, 1954, 2041, 2388
BsaWI WCCGGW 3 cut(s) 621, 740, 1792
BsaXI ACNNNNNCTCC 6 cut(s) 777, 807, 1146, 1176, 2216, 2246
Bsc4I CCNNNNNNNGG 8 cut(s) 740, 747, 807, 1200, 1338, 1339, 1780, 1946
Bse118I RCCGGY 1 cut(s) 740
Bse1I ACTGG 5 cut(s) 612, 646, 982, 1462, 1888
Bse3DI GCAATG 2 cut(s) 962, 1785
Bse8I GATNNNNATC 2 cut(s) 914, 2177
BseBI CCWGG 7 cut(s) 35, 72, 258, 365, 1152, 2042, 2345
BseDI CCNNGG 6 cut(s) 283, 657, 1333, 1954, 2041, 2388
BseGI GGATG 5 cut(s) 832, 954, 1195, 1666, 1789
BseJI GATNNNNATC 2 cut(s) 914, 2177
BseLI CCNNNNNNNGG 8 cut(s) 740, 747, 807, 1200, 1338, 1339, 1780, 1946
BseMI GCAATG 2 cut(s) 962, 1785
BseMII CTCAG 5 cut(s) 794, 1176, 1380, 1698, 1884
BseNI ACTGG 5 cut(s) 612, 646, 982, 1462, 1888
BseRI GAGGAG 4 cut(s) 161, 428, 1314, 1482
BseSI GKGCMC 1 cut(s) 1615
BsgI GTGCAG 1 cut(s) 194
Bsh1236I CGCG 4 cut(s) 659, 963, 2005, 2403
BshFI GGCC 7 cut(s) 317, 611, 1866, 2322, 2387, 2413, 2483
BshNI GGYRCC 2 cut(s) 2141, 2518
BshTI ACCGGT 1 cut(s) 740
BsiHKAI GWGCWC 2 cut(s) 1615, 1644
BsiHKCI CYCGRG 1 cut(s) 2060
BsiSI CCGG 7 cut(s) 622, 741, 801, 1496, 1546, 1793, 2484
BslFI GGGAC 7 cut(s) 50, 611, 721, 1180, 2179, 2254, 2412
BslI CCNNNNNNNGG 8 cut(s) 740, 747, 807, 1200, 1338, 1339, 1780, 1946
BsmAI GTCTC 4 cut(s) 229, 1556, 1874, 2032
BsmBI CGTCTC 2 cut(s) 229, 1556
BsmFI GGGAC 7 cut(s) 50, 611, 721, 1180, 2179, 2254, 2412
BsmI GAATGC 3 cut(s) 666, 1272, 1573
BsnI GGCC 7 cut(s) 317, 611, 1866, 2322, 2387, 2413, 2483
BsoBI CYCGRG 1 cut(s) 2060
Bsp1286I GDGCHC 4 cut(s) 291, 1026, 1615, 1644
Bsp19I CCATGG 1 cut(s) 2388
BspACI CCGC 9 cut(s) 68, 318, 657, 659, 961, 1107, 1676, 1697, 1804
BspANI GGCC 7 cut(s) 317, 611, 1866, 2322, 2387, 2413, 2483
BspCNI CTCAG 5 cut(s) 793, 1177, 1381, 1699, 1885
BspFNI CGCG 4 cut(s) 659, 963, 2005, 2403
BspHI TCATGA 1 cut(s) 1825
BspLI GGNNCC 9 cut(s) 362, 655, 1059, 1286, 1453, 2073, 2143, 2242, 2520
BspMAI CTGCAG 4 cut(s) 1282, 1879, 1964, 2026
BspOI GCTAGC 2 cut(s) 51, 2537
BspPI GGATC 9 cut(s) 197, 542, 812, 1279, 1292, 1446, 1459, 2173, 2330
BspT107I GGYRCC 2 cut(s) 2141, 2518
BsrDI GCAATG 2 cut(s) 962, 1785
BsrFI RCCGGY 1 cut(s) 740
BsrI ACTGG 5 cut(s) 612, 646, 982, 1462, 1888
BssAI RCCGGY 1 cut(s) 740
BssECI CCNNGG 6 cut(s) 283, 657, 1333, 1954, 2041, 2388
BssNI GRCGYC 1 cut(s) 1549
BssSI CACGAG 1 cut(s) 1025
BssT1I CCWWGG 2 cut(s) 283, 2388
Bst2BI CACGAG 1 cut(s) 1025
Bst2UI CCWGG 7 cut(s) 35, 72, 258, 365, 1152, 2042, 2345
Bst6I CTCTTC 1 cut(s) 222
BstACI GRCGYC 1 cut(s) 1549
BstAPI GCANNNNNTGC 2 cut(s) 672, 1595
BstDEI CTNAG 5 cut(s) 780, 1185, 1389, 1707, 1893
BstDSI CCRYGG 4 cut(s) 657, 1333, 1954, 2388
BstF5I GGATG 5 cut(s) 832, 954, 1195, 1666, 1789
BstFNI CGCG 4 cut(s) 659, 963, 2005, 2403
BstH2I RGCGCY 1 cut(s) 337
BstHHI GCGC 5 cut(s) 336, 1038, 1367, 2005, 2405
BstMAI GTCTC 4 cut(s) 229, 1556, 1874, 2032
BstNI CCWGG 7 cut(s) 35, 72, 258, 365, 1152, 2042, 2345
BstNSI RCATGY 5 cut(s) 99, 373, 847, 1274, 1648
BstSCI CCNGG 8 cut(s) 33, 70, 256, 363, 800, 1150, 2040, 2343
BstSFI CTRYAG 5 cut(s) 1161, 1278, 1875, 1960, 2022
BstSLI GKGCMC 1 cut(s) 1615
BstUI CGCG 4 cut(s) 659, 963, 2005, 2403
BstV2I GAAGAC 3 cut(s) 825, 1590, 1781
BstX2I RGATCY 6 cut(s) 202, 547, 1284, 1451, 1751, 2178
BstYI RGATCY 6 cut(s) 202, 547, 1284, 1451, 1751, 2178
BsuI GTATCC 1 cut(s) 2264
BsuRI GGCC 7 cut(s) 317, 611, 1866, 2322, 2387, 2413, 2483
BtgI CCRYGG 4 cut(s) 657, 1333, 1954, 2388
BtgZI GCGATG 1 cut(s) 1111
BtrI CACGTC 1 cut(s) 823
BtsCI GGATG 5 cut(s) 832, 954, 1195, 1666, 1789
BtsIMutI CAGTG 4 cut(s) 270, 1186, 1455, 1989
CaiI CAGNNNCTG 2 cut(s) 476, 1883
CciI TCATGA 1 cut(s) 1825
CfoI GCGC 5 cut(s) 336, 1038, 1367, 2005, 2405
Cfr10I RCCGGY 1 cut(s) 740
Cfr13I GGNCC 4 cut(s) 609, 928, 1729, 2241
Cfr42I CCGCGG 1 cut(s) 660
CseI GACGC 4 cut(s) 1394, 1538, 1595, 2409
Csp6I GTAC 5 cut(s) 975, 1978, 2142, 2348, 2519
CspAI ACCGGT 1 cut(s) 740
CviQI GTAC 5 cut(s) 975, 1978, 2142, 2348, 2519
DdeI CTNAG 5 cut(s) 780, 1185, 1389, 1707, 1893
EaeI YGGCCR 3 cut(s) 315, 1864, 2385
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
EciI GGCGGA 1 cut(s) 1793
Eco130I CCWWGG 2 cut(s) 283, 2388
Eco147I AGGCCT 2 cut(s) 2322, 2413
Eco24I GRGCYC 2 cut(s) 291, 1026
Eco32I GATATC 1 cut(s) 1372
Eco47I GGWCC 3 cut(s) 928, 1729, 2241
Eco47III AGCGCT 1 cut(s) 335
Eco57I CTGAAG 3 cut(s) 528, 687, 1602
Eco88I CYCGRG 1 cut(s) 2060
EcoRI GAATTC 1 cut(s) 393
EcoRII CCWGG 7 cut(s) 33, 70, 256, 363, 1150, 2040, 2343
EcoRV GATATC 1 cut(s) 1372
EcoT14I CCWWGG 2 cut(s) 283, 2388
EcoT22I ATGCAT 2 cut(s) 677, 1272
EcoT38I GRGCYC 2 cut(s) 291, 1026
ErhI CCWWGG 2 cut(s) 283, 2388
Esp3I CGTCTC 2 cut(s) 229, 1556
FalI AAGNNNNNCTT 2 cut(s) 2310, 2342
FaqI GGGAC 7 cut(s) 50, 611, 721, 1180, 2179, 2254, 2412
FauI CCCGC 1 cut(s) 75
FauNDI CATATG 1 cut(s) 343
FbaI TGATCA 2 cut(s) 1689, 2353
FokI GGATG 5 cut(s) 839, 961, 1202, 1673, 1796
FriOI GRGCYC 2 cut(s) 291, 1026
GlaI GCGC 5 cut(s) 335, 1037, 1366, 2004, 2404
GsuI CTGGAG 1 cut(s) 1134
HaeII RGCGCY 1 cut(s) 337
HaeIII GGCC 7 cut(s) 317, 611, 1866, 2322, 2387, 2413, 2483
HapII CCGG 7 cut(s) 622, 741, 801, 1496, 1546, 1793, 2484
HgaI GACGC 4 cut(s) 1394, 1538, 1595, 2409
HhaI GCGC 5 cut(s) 336, 1038, 1367, 2005, 2405
Hin1I GRCGYC 1 cut(s) 1549
Hin6I GCGC 5 cut(s) 334, 1036, 1365, 2003, 2403
HinP1I GCGC 5 cut(s) 334, 1036, 1365, 2003, 2403
HindIII AAGCTT 2 cut(s) 752, 852
HinfI GANTC 5 cut(s) 347, 590, 730, 1099, 2578
HpaII CCGG 7 cut(s) 622, 741, 801, 1496, 1546, 1793, 2484
HphI GGTGA 5 cut(s) 389, 755, 1076, 1472, 2245
Hpy166II GTNNAC 4 cut(s) 723, 924, 1006, 1613
Hpy188I TCNGA 9 cut(s) 478, 547, 636, 1376, 1660, 1756, 1930, 1941, 2134
Hpy8I GTNNAC 4 cut(s) 723, 924, 1006, 1613
HpyCH4IV ACGT 2 cut(s) 436, 822
HpyF3I CTNAG 5 cut(s) 780, 1185, 1389, 1707, 1893
HpySE526I ACGT 2 cut(s) 436, 822
Hsp92I GRCGYC 1 cut(s) 1549
HspAI GCGC 5 cut(s) 334, 1036, 1365, 2003, 2403
KpnI GGTACC 2 cut(s) 2145, 2522
Ksp22I TGATCA 2 cut(s) 1689, 2353
KspI CCGCGG 1 cut(s) 660
LmnI GCTCC 4 cut(s) 624, 862, 986, 1154
LweI GCATC 5 cut(s) 636, 817, 1154, 1180, 1932
MaeII ACGT 2 cut(s) 436, 822
MaeIII GTNAC 2 cut(s) 1795, 1844
MflI RGATCY 6 cut(s) 202, 547, 1284, 1451, 1751, 2178
MhlI GDGCHC 4 cut(s) 291, 1026, 1615, 1644
MlsI TGGCCA 2 cut(s) 1866, 2387
MluCI AATT 7 cut(s) 393, 419, 838, 882, 1767, 1829, 1922
MluNI TGGCCA 2 cut(s) 1866, 2387
MlyI GAGTC 1 cut(s) 341
MmeI TCCRAC 3 cut(s) 946, 1127, 2407
Mox20I TGGCCA 2 cut(s) 1866, 2387
Mph1103I ATGCAT 2 cut(s) 677, 1272
MroXI GAANNNNTTC 2 cut(s) 700, 1833
MscI TGGCCA 2 cut(s) 1866, 2387
MseI TTAA 5 cut(s) 483, 576, 993, 2121, 2420
MslI CAYNNNNRTG 3 cut(s) 1994, 2258, 2381
Msp20I TGGCCA 2 cut(s) 1866, 2387
MspA1I CMGCKG 4 cut(s) 659, 1160, 1277, 1322
MspI CCGG 7 cut(s) 622, 741, 801, 1496, 1546, 1793, 2484
MspR9I CCNGG 8 cut(s) 35, 72, 258, 365, 802, 1152, 2042, 2345
Mva1269I GAATGC 3 cut(s) 666, 1272, 1573
MvaI CCWGG 7 cut(s) 35, 72, 258, 365, 1152, 2042, 2345
MvnI CGCG 4 cut(s) 659, 963, 2005, 2403
NciI CCSGG 1 cut(s) 802
NcoI CCATGG 1 cut(s) 2388
NdeI CATATG 1 cut(s) 343
NheI GCTAGC 2 cut(s) 47, 2533
NlaIV GGNNCC 9 cut(s) 362, 655, 1059, 1286, 1453, 2073, 2143, 2242, 2520
NsiI ATGCAT 2 cut(s) 677, 1272
NspI RCATGY 5 cut(s) 99, 373, 847, 1274, 1648
PaeI GCATGC 3 cut(s) 99, 847, 1274
PagI TCATGA 1 cut(s) 1825
PceI AGGCCT 2 cut(s) 2322, 2413
PciI ACATGT 2 cut(s) 369, 1644
PctI GAATGC 3 cut(s) 666, 1272, 1573
PdmI GAANNNNTTC 2 cut(s) 700, 1833
PfeI GAWTC 4 cut(s) 590, 730, 1099, 2578
PflFI GACNNNGTC 2 cut(s) 272, 1541
PfoI TCCNGGA 2 cut(s) 363, 1150
PinAI ACCGGT 1 cut(s) 740
PleI GAGTC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 341
PscI ACATGT 2 cut(s) 369, 1644
Psp6I CCWGG 7 cut(s) 33, 70, 256, 363, 1150, 2040, 2343
PspGI CCWGG 7 cut(s) 33, 70, 256, 363, 1150, 2040, 2343
PspN4I GGNNCC 9 cut(s) 362, 655, 1059, 1286, 1453, 2073, 2143, 2242, 2520
PspPI GGNCC 4 cut(s) 609, 928, 1729, 2241
PstI CTGCAG 4 cut(s) 1282, 1879, 1964, 2026
PstNI CAGNNNCTG 2 cut(s) 476, 1883
PsuI RGATCY 6 cut(s) 202, 547, 1284, 1451, 1751, 2178
PsyI GACNNNGTC 2 cut(s) 272, 1541
PvuII CAGCTG 3 cut(s) 1160, 1277, 1322
RsaI GTAC 5 cut(s) 976, 1979, 2143, 2349, 2520
RsaNI GTAC 5 cut(s) 975, 1978, 2142, 2348, 2519
RseI CAYNNNNRTG 3 cut(s) 1994, 2258, 2381
SacII CCGCGG 1 cut(s) 660
SaqAI TTAA 5 cut(s) 483, 576, 993, 2121, 2420
Sau96I GGNCC 4 cut(s) 609, 928, 1729, 2241
SchI GAGTC 1 cut(s) 341
ScrFI CCNGG 8 cut(s) 35, 72, 258, 365, 802, 1152, 2042, 2345
SduI GDGCHC 4 cut(s) 291, 1026, 1615, 1644
SfaNI GCATC 5 cut(s) 636, 817, 1154, 1180, 1932
SfcI CTRYAG 5 cut(s) 1161, 1278, 1875, 1960, 2022
Sfr303I CCGCGG 1 cut(s) 660
SgrAI CRCCGGYG 1 cut(s) 740
SgrBI CCGCGG 1 cut(s) 660
SinI GGWCC 3 cut(s) 928, 1729, 2241
SmiMI CAYNNNNRTG 3 cut(s) 1994, 2258, 2381
SmlI CTYRAG 3 cut(s) 1553, 2149, 2544
SmoI CTYRAG 3 cut(s) 1553, 2149, 2544
SphI GCATGC 3 cut(s) 99, 847, 1274
Sse9I AATT 7 cut(s) 393, 419, 838, 882, 1767, 1829, 1922
SseBI AGGCCT 2 cut(s) 2322, 2413
SsiI CCGC 9 cut(s) 68, 318, 657, 659, 961, 1107, 1676, 1697, 1804
StuI AGGCCT 2 cut(s) 2322, 2413
StyD4I CCNGG 8 cut(s) 33, 70, 256, 363, 800, 1150, 2040, 2343
StyI CCWWGG 2 cut(s) 283, 2388
TaiI ACGT 2 cut(s) 439, 825
TaqI TCGA 3 cut(s) 411, 519, 918
TasI AATT 7 cut(s) 393, 419, 838, 882, 1767, 1829, 1922
TauI GCSGC 3 cut(s) 320, 963, 1700
TfiI GAWTC 4 cut(s) 590, 730, 1099, 2578
Tru1I TTAA 5 cut(s) 483, 576, 993, 2121, 2420
Tru9I TTAA 5 cut(s) 483, 576, 993, 2121, 2420
TscAI CASTG 4 cut(s) 277, 1186, 1462, 1996
TspDTI ATGAA 4 cut(s) 17, 848, 1814, 1842
TspGWI ACGGA 3 cut(s) 52, 1763, 1943
TspRI CASTG 4 cut(s) 277, 1186, 1462, 1996
Tth111I GACNNNGTC 2 cut(s) 272, 1541
VneI GTGCAC 1 cut(s) 1611
VpaK11BI GGWCC 3 cut(s) 928, 1729, 2241
XapI RAATTY 4 cut(s) 393, 882, 1767, 1829
XbaI TCTAGA 1 cut(s) 886
XceI RCATGY 5 cut(s) 99, 373, 847, 1274, 1648
XcmI CCANNNNNNNNNTGG 1 cut(s) 2264
XmnI GAANNNNTTC 2 cut(s) 700, 1833
Zsp2I ATGCAT 2 cut(s) 677, 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.