Rh4CG236500

Pyruvate phosphate dikinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0004385
Physical Location & Seq
Forward (+)
48066293 .. 48072623
6331 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG236500.1

Sequence Viewer

Length: 2883 bp
ATGTCCTCCACAGTGAAAGGTTTACTCTTGAGGACTTCACCGGAGGTAGCTTATAGACACAGAATGTTCAACGATCGTTCTTTCCTCAACGGCGTGAACGGTGGCTCCACCCGAGCCGGCCAGACCCGGCATCAGCCTCACGTTGCTACCATAACAAATGGGATCACCAACCCCAGTCCTAAGAAAAATGAGCTCCAGAGCCAGATCGGAGCCCAGCCTATGATGTCACCAGTTTTGGACCCGAGTACTCCAACAACGCACAAGAGGGTTTTTACTTTTGGGAAGGGAAGGAGTGAAGGAAACAAGGGCATGAAGTCACTGTTGGGAGGGAAGGGTGCAAACCTGGCTGAAATGGCTAGCATCGGGCTATCCGTGCCACCCGGGCTTACTATCTCAACAGAAGCATGCCAAGAGTATCAGGAGAATGGTAAAGAATTACCGGAAGGTTTGTGGGAGGAGATACTAGAAGGCTTGGATTATGTGCAGAAGACTATGGGAGCTACTCTTGGTGACCCTTCCAAGCCCCTCCTTGTCTCTGTTCGCTCTGGTGCAGCGATTTCTATGCCTGGCATGATGGACACTGTCCTCAACCTTGGACTGAATGATGAGGTGGTTGCTGGTTTAGCTGTAAAAAGTGGGGAGCGCTTTGCATATGACTCATACAGACGTTTCCTAGACATGTTTGGAGATGTTGTTATGGAAATTCCACACTCATCATTTGGGGAGAAGTTGGAAAACTACAAACACCAAAAAGGAGTTGAACTTGATACAGAGCTAACAGCTTCTGATCTCAAAGAGCTGGTAGAACAATACAAAAGTGTCTATCTTGAAGCTACGGGTCAAAAATTCCCATCAGATCCAAAACAACAGCTAGTGTTGGCTATTAAAGCAGTTTTTAATTCTTGGGACAGCCCGAGGGCCAACAAATACCGGAGCATCAATCAGATAACTGGTTTAAAGGGAACTGCAGTTAACATTCAAAGCATGGCTTTTGGGAACATGGGAAGCACTTCAGGGACAGGTGTTCTATTCACTAGAAATCCAAGCACTGGTGAAAAGAAGCTCTATGGGGAGTTTCTAGTCAATGCTCAGGGAGAAGATGTAGTTGCCGGTATTAGGACACCAGAACCTTTGGATACCATGAAAAATTGCATGCCAGAAGCTTACAAGGAGCTTGTGGAGAACTGTGAAATTCTAGAAAGACATTACAAAGATATGATGGACATTGAGTTCACTGTCCAAGAAAATAGATTATGGATGTTGCAATGTCGTACTGGAAAGCGTACTGGTAAAGGTGCGGTCAAGATAGCTGTAGACATGGTTAATGAAGGGCTTGTTGAAAAGCGCTCCGCAATCAAGATGGTGGAACCACAACATGTTGACCAACTTCTACACCCTCAGTTTGAGGATCCGACAGCATACAAAGACAAAGTGCTTGCCACTGGCTTGCCTGCATCACCAGGAGCAGCAGTAGGGCAAATCGTGTTCAGTGCAGAGGACGCTGAAGCATGGCATGCACAAGGAAAGAAGACCATTTTGGTAAGGACGGAGACCAGCCCGGAAGATATTGGGGGAATGCATGCATCTGCGGGGATCTTGACTGCTAGAGGTGGCATGACATCTCACGCAGCTGTTGTCGCTCGTGGATGGGGTAAATGTTGTGTTTCTGGATGCTCTGATATCCGAGTAAATGATGATGAGAAGGTTGTTGTGATTGGGGACACTTTGTTTAGAGAAGGAGAATGGCTCTCCCTCAATGGAAACACCGGTGAAGTCATATCAGGAAAACAGCCACTTTCTCCTCCAGCTTTAAGCGGTGATTTGGAAACCTTTATGTCTTGGGCTGATAGCTTTAGGCGTCTCAAGGTTATGGCAAATGCCGACACACCTGAAGATGCACTAACAGCAAGAAACAATGGTGCACAAGGGATTGGACTTTGCAGGACAGAGCACATGTTCTTTGCTTCAGAGGAAAGAATTAAGTCAGTAAGAAAGATGATCATGGCAAATACACTTGAGCAGAGGAAGGCAGCATTGGATCTCTTACTACCATATCAGAGATCTGACTTTGAAGGAATTTTCCGTGCAATGGATGGTCTTCCGGTAACCATACGATTGTTAGACCCTCCACTTCATGAGTTTCTTCCAGAAGGTGACCTAGAACAGATTGTTAAAGAAGTAGCTGCAGAGACTGACATGACTGAGGATGATGTTTACTCGAGAGTTGATAAATTAACAGAAGTAAACCCAATGCTTGGTTTCCGGGGCTGCAGGCTAGGGATTTCGTACCCAGAACTCACAGAAATGCAAGCACGTGCAGTCTTTCAGGCTGCAGTCTTGATGAGCAACCAGGGTGTCAAAGTTTTCCCTGAGATAATGGTTCCTCTTGTTGGATCACCTCAGGAACTGGGACACCAAGTGACTTTAATCCGGAGTGTTGCAAAGAAAGTGTTCTCTGAGATGGGTACTACCTTGAGTTTCAAGGTTGGAACCATGATTGAGATTCCCAGAGCTGCTCTGATTGCAGATGAGATTGCAAAAGAAGCTGAGTTCTTCTCTTTTGGAACCAATGATCTCACCCAAATGACCTTTGGGTACAGTAGAGATGATGTGGGCAAGTTCCTACCTATTTATTTGTCCAAAGGCATTCTTCAAAATGATCCATTTGAGGTACTGGATCAAAGAGGAGTTGGGCAGCTCGTCAAGATGGCCACAGAAAAGGGTCGTGCAGCTAAGCCAAGCTTAAAGGTTGGAATATGCGGTGAGCATGGTGGGGAACCTTCTTCTGTTGCATTTTTCGCAGAGGCCGGACTAGACTATGTTTCATGTTCTCCATTCAGGGTACCTATTGCAAGGCTAGCAGCAGCTCAAGTTGCCGTCTGA

Protein Analysis

960

Amino Acids

104.72

Weight (kDa)

5.62

Isoelectric Point (pI)

37.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPDK_N PF01326 106 - 142 7.7e-07 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N PF01326 144 - 377 3.5e-23 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PPDK_N PF01326 388 - 443 1.9e-10 Pyruvate phosphate dikinase, AMP/ATP-binding domain
PEP-utilizers PF00391 509 - 590 2e-22 PEP-utilising enzyme, mobile domain
PEP-utilizers_C PF02896 605 - 959 4.9e-92 PEP-utilising enzyme, PEP-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2842
AccB1I GGYRCC 1 cut(s) 2842
AccB7I CCANNNNNTGG 2 cut(s) 1049, 2255
AccI GTMKAC 1 cut(s) 1314
AccIII TCCGGA 1 cut(s) 2430
AciI CCGC 5 cut(s) 1298, 1350, 1589, 1815, 2760
AclWI GGATC 9 cut(s) 170, 851, 1403, 1416, 1601, 2046, 2401, 2654, 2685
AcoI YGGCCR 2 cut(s) 118, 2709
AcsI RAATTY 4 cut(s) 702, 845, 1191, 2076
AcuI CTGAAG 4 cut(s) 996, 1524, 1911, 1950
AcvI CACGTG 1 cut(s) 2315
AcyI GRCGYC 1 cut(s) 1858
AdeI CACNNNGTG 1 cut(s) 2419
AfaI GTAC 8 cut(s) 247, 1273, 1285, 2288, 2467, 2597, 2673, 2844
AfeI AGCGCT 2 cut(s) 644, 1346
AfiI CCNNNNNNNGG 5 cut(s) 1049, 1116, 2089, 2255, 2390
AflIII ACRYGT 3 cut(s) 678, 1375, 1953
AgeI ACCGGT 1 cut(s) 1766
AgsI TTSAA 8 cut(s) 70, 761, 830, 980, 1340, 2072, 2482, 2654
AjnI CCWGG 4 cut(s) 342, 565, 1459, 2349
AjuI GAANNNNNNNTTGG 6 cut(s) 305, 337, 1520, 1552, 2018, 2050
AloI GAACNNNNNNTCC 4 cut(s) 89, 121, 1214, 1246
Alw21I GWGCWC 3 cut(s) 195, 1924, 1953
Alw26I GTCTC 4 cut(s) 538, 1544, 1865, 2183
Alw44I GTGCAC 1 cut(s) 1920
AlwI GGATC 9 cut(s) 170, 851, 1403, 1416, 1601, 2046, 2401, 2654, 2685
AlwNI CAGNNNCTG 3 cut(s) 785, 2192, 2407
Ama87I CYCGRG 5 cut(s) 111, 241, 380, 913, 2218
Aor13HI TCCGGA 1 cut(s) 2430
Aor51HI AGCGCT 2 cut(s) 644, 1346
AoxI GGCC 4 cut(s) 118, 918, 2709, 2805
ApaLI GTGCAC 1 cut(s) 1920
ApoI RAATTY 4 cut(s) 702, 845, 1191, 2076
AsiGI ACCGGT 1 cut(s) 1766
Asp700I GAANNNNTTC 2 cut(s) 1009, 2450
Asp718I GGTACC 1 cut(s) 2842
AspLEI GCGC 2 cut(s) 645, 1347
AspS9I GGNCC 2 cut(s) 238, 918
AsuC2I CCSGG 5 cut(s) 127, 381, 382, 1559, 2264
AsuNHI GCTAGC 2 cut(s) 356, 2857
AvaI CYCGRG 5 cut(s) 111, 241, 380, 913, 2218
AvaII GGWCC 1 cut(s) 238
AxyI CCTNAGG 1 cut(s) 2400
BaeGI GKGCMC 1 cut(s) 1924
BalI TGGCCA 1 cut(s) 2711
BamHI GGATCC 1 cut(s) 1408
BanI GGYRCC 1 cut(s) 2842
BanII GRGCYC 2 cut(s) 195, 214
BauI CACGAG 1 cut(s) 1641
BbrPI CACGTG 1 cut(s) 2315
BbsI GAAGAC 3 cut(s) 494, 1535, 2090
Bbv12I GWGCWC 3 cut(s) 195, 1924, 1953
BccI CCATC 8 cut(s) 568, 859, 1213, 1354, 1641, 2087, 2455, 2701
BceAI ACGGC 2 cut(s) 106, 2861
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 4 cut(s) 344, 567, 1461, 2351
BciVI GTATCC 1 cut(s) 1129
BclI TGATCA 1 cut(s) 1998
BcnI CCSGG 5 cut(s) 127, 381, 382, 1559, 2264
BcoDI GTCTC 4 cut(s) 538, 1544, 1865, 2183
BfmI CTRYAG 5 cut(s) 966, 1311, 2184, 2269, 2331
BfoI RGCGCY 2 cut(s) 646, 1348
BfuI GTATCC 1 cut(s) 1129
BglI GCCNNNNNGGC 1 cut(s) 382
BglII AGATCT 1 cut(s) 2060
BlpI GCTNAGC 1 cut(s) 2733
BmcAI AGTACT 1 cut(s) 247
Bme1390I CCNGG 9 cut(s) 127, 344, 381, 382, 567, 1461, 1559, 2264, 2351
Bme18I GGWCC 1 cut(s) 238
BmeT110I CYCGRG 5 cut(s) 111, 241, 380, 913, 2218
BmgT120I GGNCC 2 cut(s) 238, 918
BmrFI CCNGG 9 cut(s) 127, 344, 381, 382, 567, 1461, 1559, 2264, 2351
BmrI ACTGGG 2 cut(s) 168, 2417
BmsI GCATC 7 cut(s) 139, 369, 945, 1463, 1592, 1661, 1885
BmtI GCTAGC 2 cut(s) 360, 2861
BmuI ACTGGG 2 cut(s) 168, 2417
BpiI GAAGAC 3 cut(s) 494, 1535, 2090
BplI GAGNNNNNCTC 4 cut(s) 1731, 1763, 2540, 2572
BpmI CTGGAG 2 cut(s) 179, 1788
Bpu10I CCTNAGC 1 cut(s) 1089
Bpu1102I GCTNAGC 1 cut(s) 2733
BpuEI CTTGAG 5 cut(s) 49, 1847, 2036, 2494, 2853
BpuMI CCSGG 5 cut(s) 127, 381, 382, 1559, 2264
BsaAI YACGTR 1 cut(s) 2315
BsaHI GRCGYC 1 cut(s) 1858
BsaI GGTCTC 1 cut(s) 1544
BsaJI CCNNGG 5 cut(s) 380, 592, 914, 2263, 2350
BsaWI WCCGGW 6 cut(s) 40, 439, 930, 1766, 2101, 2430
BsaXI ACNNNNNCTCC 8 cut(s) 89, 119, 678, 708, 1086, 1116, 1455, 1485
Bsc4I CCNNNNNNNGG 5 cut(s) 1049, 1116, 2089, 2255, 2390
Bse118I RCCGGY 3 cut(s) 116, 1109, 1766
Bse1I ACTGG 9 cut(s) 174, 230, 955, 1054, 1279, 1291, 1447, 2412, 2679
Bse21I CCTNAGG 1 cut(s) 2400
Bse3DI GCAATG 2 cut(s) 1271, 2094
BseAI TCCGGA 1 cut(s) 2430
BseBI CCWGG 4 cut(s) 344, 567, 1461, 2351
BseDI CCNNGG 5 cut(s) 380, 592, 914, 2263, 2350
BseGI GGATG 5 cut(s) 1263, 1652, 1676, 2098, 2212
BseLI CCNNNNNNNGG 5 cut(s) 1049, 1116, 2089, 2255, 2390
BseMI GCAATG 2 cut(s) 1271, 2094
BseMII CTCAG 7 cut(s) 1103, 1412, 2193, 2361, 2414, 2448, 2538
BseNI ACTGG 9 cut(s) 174, 230, 955, 1054, 1279, 1291, 1447, 2412, 2679
BseRI GAGGAG 3 cut(s) 470, 1791, 2700
BseSI GKGCMC 1 cut(s) 1924
BseYI CCCAGC 1 cut(s) 213
BsgI GTGCAG 5 cut(s) 503, 570, 1512, 2337, 2748
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 4 cut(s) 120, 920, 2711, 2807
BshNI GGYRCC 1 cut(s) 2842
BshTI ACCGGT 1 cut(s) 1766
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 3 cut(s) 195, 1924, 1953
BsiHKCI CYCGRG 5 cut(s) 111, 241, 380, 913, 2218
BslFI GGGAC 4 cut(s) 920, 1030, 1733, 2424
BslI CCNNNNNNNGG 5 cut(s) 1049, 1116, 2089, 2255, 2390
BsmAI GTCTC 4 cut(s) 538, 1544, 1865, 2183
BsmBI CGTCTC 1 cut(s) 1865
BsmFI GGGAC 4 cut(s) 920, 1030, 1733, 2424
BsmI GAATGC 2 cut(s) 1581, 2646
BsnI GGCC 4 cut(s) 120, 920, 2711, 2807
Bso31I GGTCTC 1 cut(s) 1544
BsoBI CYCGRG 5 cut(s) 111, 241, 380, 913, 2218
Bsp1286I GDGCHC 4 cut(s) 195, 214, 1924, 1953
Bsp13I TCCGGA 1 cut(s) 2430
Bsp1720I GCTNAGC 1 cut(s) 2733
BspACI CCGC 5 cut(s) 1298, 1350, 1589, 1815, 2760
BspANI GGCC 4 cut(s) 120, 920, 2711, 2807
BspCNI CTCAG 7 cut(s) 1102, 1411, 2194, 2362, 2413, 2449, 2539
BspEI TCCGGA 1 cut(s) 2430
BspHI TCATGA 1 cut(s) 2134
BspMAI CTGCAG 4 cut(s) 970, 2188, 2273, 2335
BspOI GCTAGC 2 cut(s) 360, 2861
BspPI GGATC 9 cut(s) 170, 851, 1403, 1416, 1601, 2046, 2401, 2654, 2685
BspT107I GGYRCC 1 cut(s) 2842
BspTNI GGTCTC 1 cut(s) 1544
BsrDI GCAATG 2 cut(s) 1271, 2094
BsrFI RCCGGY 3 cut(s) 116, 1109, 1766
BsrI ACTGG 9 cut(s) 174, 230, 955, 1054, 1279, 1291, 1447, 2412, 2679
BssAI RCCGGY 3 cut(s) 116, 1109, 1766
BssECI CCNNGG 5 cut(s) 380, 592, 914, 2263, 2350
BssNI GRCGYC 1 cut(s) 1858
BssSI CACGAG 1 cut(s) 1641
BssT1I CCWWGG 1 cut(s) 592
Bst2BI CACGAG 1 cut(s) 1641
Bst2UI CCWGG 4 cut(s) 344, 567, 1461, 2351
Bst4CI ACNGT 7 cut(s) 13, 101, 321, 583, 1187, 1237, 2600
BstACI GRCGYC 1 cut(s) 1858
BstAPI GCANNNNNTGC 1 cut(s) 1514
BstBAI YACGTR 1 cut(s) 2315
BstDEI CTNAG 9 cut(s) 180, 1089, 1398, 2202, 2370, 2400, 2457, 2547, 2733
BstEII GGTNACC 3 cut(s) 509, 2104, 2153
BstF5I GGATG 5 cut(s) 1263, 1652, 1676, 2098, 2212
BstH2I RGCGCY 2 cut(s) 646, 1348
BstHHI GCGC 2 cut(s) 645, 1347
BstMAI GTCTC 4 cut(s) 538, 1544, 1865, 2183
BstMCI CGRYCG 1 cut(s) 76
BstNI CCWGG 4 cut(s) 344, 567, 1461, 2351
BstNSI RCATGY 7 cut(s) 408, 682, 1156, 1379, 1517, 1583, 1957
BstPI GGTNACC 3 cut(s) 509, 2104, 2153
BstSCI CCNGG 9 cut(s) 125, 342, 379, 380, 565, 1459, 1557, 2262, 2349
BstSFI CTRYAG 5 cut(s) 966, 1311, 2184, 2269, 2331
BstSLI GKGCMC 1 cut(s) 1924
BstV2I GAAGAC 3 cut(s) 494, 1535, 2090
BstX2I RGATCY 5 cut(s) 856, 1408, 1593, 2038, 2060
BstYI RGATCY 5 cut(s) 856, 1408, 1593, 2038, 2060
Bsu36I CCTNAGG 1 cut(s) 2400
BsuI GTATCC 1 cut(s) 1129
BsuRI GGCC 4 cut(s) 120, 920, 2711, 2807
BtsCI GGATG 5 cut(s) 1263, 1652, 1676, 2098, 2212
BtsIMutI CAGTG 7 cut(s) 18, 317, 579, 1047, 1233, 1440, 1495
CaiI CAGNNNCTG 3 cut(s) 785, 2192, 2407
CciI TCATGA 1 cut(s) 2134
CfoI GCGC 2 cut(s) 645, 1347
Cfr10I RCCGGY 3 cut(s) 116, 1109, 1766
Cfr13I GGNCC 2 cut(s) 238, 918
Cfr9I CCCGGG 1 cut(s) 380
CseI GACGC 2 cut(s) 1508, 1847
Csp6I GTAC 8 cut(s) 246, 1272, 1284, 2287, 2466, 2596, 2672, 2843
CspAI ACCGGT 1 cut(s) 1766
CviQI GTAC 8 cut(s) 246, 1272, 1284, 2287, 2466, 2596, 2672, 2843
DdeI CTNAG 9 cut(s) 180, 1089, 1398, 2202, 2370, 2400, 2457, 2547, 2733
DraI TTTAAA 1 cut(s) 957
DraIII CACNNNGTG 1 cut(s) 2419
EaeI YGGCCR 2 cut(s) 118, 2709
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 592
Eco24I GRGCYC 2 cut(s) 195, 214
Eco31I GGTCTC 1 cut(s) 1544
Eco32I GATATC 1 cut(s) 1681
Eco47I GGWCC 1 cut(s) 238
Eco47III AGCGCT 2 cut(s) 644, 1346
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 4 cut(s) 996, 1524, 1911, 1950
Eco72I CACGTG 1 cut(s) 2315
Eco81I CCTNAGG 1 cut(s) 2400
Eco88I CYCGRG 5 cut(s) 111, 241, 380, 913, 2218
Eco91I GGTNACC 3 cut(s) 509, 2104, 2153
EcoICRI GAGCTC 1 cut(s) 193
EcoO65I GGTNACC 3 cut(s) 509, 2104, 2153
EcoRII CCWGG 4 cut(s) 342, 565, 1459, 2349
EcoRV GATATC 1 cut(s) 1681
EcoT14I CCWWGG 1 cut(s) 592
EcoT22I ATGCAT 2 cut(s) 1581, 1585
EcoT38I GRGCYC 2 cut(s) 195, 214
ErhI CCWWGG 1 cut(s) 592
Esp3I CGTCTC 1 cut(s) 1865
FalI AAGNNNNNCTT 6 cut(s) 973, 1005, 2634, 2666, 2726, 2758
FaqI GGGAC 4 cut(s) 920, 1030, 1733, 2424
FauI CCCGC 1 cut(s) 1582
FauNDI CATATG 1 cut(s) 652
FbaI TGATCA 1 cut(s) 1998
FblI GTMKAC 1 cut(s) 1314
FokI GGATG 5 cut(s) 1270, 1659, 1683, 2105, 2219
FriOI GRGCYC 2 cut(s) 195, 214
GlaI GCGC 2 cut(s) 644, 1346
GsaI CCCAGC 1 cut(s) 217
GsuI CTGGAG 2 cut(s) 179, 1788
HaeII RGCGCY 2 cut(s) 646, 1348
HaeIII GGCC 4 cut(s) 120, 920, 2711, 2807
HgaI GACGC 2 cut(s) 1508, 1847
HhaI GCGC 2 cut(s) 645, 1347
Hin1I GRCGYC 1 cut(s) 1858
Hin6I GCGC 2 cut(s) 643, 1345
HinP1I GCGC 2 cut(s) 643, 1345
HincII GTYRAC 2 cut(s) 973, 1381
HindII GTYRAC 2 cut(s) 973, 1381
HindIII AAGCTT 2 cut(s) 1161, 2740
HinfI GANTC 2 cut(s) 656, 2503
HpaI GTTAAC 1 cut(s) 973
Hpy166II GTNNAC 9 cut(s) 23, 97, 973, 1233, 1315, 1381, 1922, 2215, 2245
Hpy8I GTNNAC 9 cut(s) 23, 97, 973, 1233, 1315, 1381, 1922, 2215, 2245
HpyCH4III ACNGT 7 cut(s) 13, 101, 321, 583, 1187, 1237, 2600
HpyCH4IV ACGT 3 cut(s) 141, 667, 2314
HpyF3I CTNAG 9 cut(s) 180, 1089, 1398, 2202, 2370, 2400, 2457, 2547, 2733
HpySE526I ACGT 3 cut(s) 141, 667, 2314
Hsp92I GRCGYC 1 cut(s) 1858
HspAI GCGC 2 cut(s) 643, 1345
Kpn2I TCCGGA 1 cut(s) 2430
KpnI GGTACC 1 cut(s) 2846
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
Ksp22I TGATCA 1 cut(s) 1998
KspAI GTTAAC 1 cut(s) 973
LmnI GCTCC 9 cut(s) 110, 198, 209, 497, 640, 933, 1171, 1352, 1463
LweI GCATC 7 cut(s) 139, 369, 945, 1463, 1592, 1661, 1885
MaeII ACGT 3 cut(s) 141, 667, 2314
MaeIII GTNAC 6 cut(s) 225, 315, 509, 2104, 2153, 2419
MflI RGATCY 5 cut(s) 856, 1408, 1593, 2038, 2060
MhlI GDGCHC 4 cut(s) 195, 214, 1924, 1953
MlsI TGGCCA 1 cut(s) 2711
MluCI AATT 9 cut(s) 434, 702, 845, 898, 1147, 1191, 1977, 2076, 2231
MluNI TGGCCA 1 cut(s) 2711
MlyI GAGTC 1 cut(s) 650
MmeI TCCRAC 6 cut(s) 275, 711, 1436, 2371, 2467, 2731
Mox20I TGGCCA 1 cut(s) 2711
Mph1103I ATGCAT 2 cut(s) 1581, 1585
MroI TCCGGA 1 cut(s) 2430
MroNI GCCGGC 1 cut(s) 116
MroXI GAANNNNTTC 2 cut(s) 1009, 2450
MscI TGGCCA 1 cut(s) 2711
MslI CAYNNNNRTG 2 cut(s) 2303, 2582
Msp20I TGGCCA 1 cut(s) 2711
MspA1I CMGCKG 1 cut(s) 1631
MspR9I CCNGG 9 cut(s) 127, 344, 381, 382, 567, 1461, 1559, 2264, 2351
Mva1269I GAATGC 2 cut(s) 1581, 2646
MvaI CCWGG 4 cut(s) 344, 567, 1461, 2351
NaeI GCCGGC 1 cut(s) 118
NciI CCSGG 5 cut(s) 127, 381, 382, 1559, 2264
NdeI CATATG 1 cut(s) 652
NgoMIV GCCGGC 1 cut(s) 116
NheI GCTAGC 2 cut(s) 356, 2857
NmuCI GTSAC 5 cut(s) 225, 315, 509, 2153, 2419
NsiI ATGCAT 2 cut(s) 1581, 1585
NspI RCATGY 7 cut(s) 408, 682, 1156, 1379, 1517, 1583, 1957
PaeI GCATGC 4 cut(s) 408, 1156, 1517, 1583
PaeR7I CTCGAG 1 cut(s) 2218
PagI TCATGA 1 cut(s) 2134
PciI ACATGT 3 cut(s) 678, 1375, 1953
PcsI WCGNNNNNNNCGW 1 cut(s) 369
PctI GAATGC 2 cut(s) 1581, 2646
PdiI GCCGGC 1 cut(s) 118
PdmI GAANNNNTTC 2 cut(s) 1009, 2450
PfeI GAWTC 1 cut(s) 2503
PflFI GACNNNGTC 1 cut(s) 581
PflMI CCANNNNNTGG 2 cut(s) 1049, 2255
PinAI ACCGGT 1 cut(s) 1766
Ple19I CGATCG 1 cut(s) 76
PleI GAGTC 1 cut(s) 650
PmaCI CACGTG 1 cut(s) 2315
PmlI CACGTG 1 cut(s) 2315
PpsI GAGTC 1 cut(s) 650
Ppu21I YACGTR 1 cut(s) 2315
PscI ACATGT 3 cut(s) 678, 1375, 1953
Psp124BI GAGCTC 1 cut(s) 195
Psp6I CCWGG 4 cut(s) 342, 565, 1459, 2349
PspCI CACGTG 1 cut(s) 2315
PspEI GGTNACC 3 cut(s) 509, 2104, 2153
PspFI CCCAGC 1 cut(s) 213
PspGI CCWGG 4 cut(s) 342, 565, 1459, 2349
PspPI GGNCC 2 cut(s) 238, 918
PstI CTGCAG 4 cut(s) 970, 2188, 2273, 2335
PstNI CAGNNNCTG 3 cut(s) 785, 2192, 2407
PsuI RGATCY 5 cut(s) 856, 1408, 1593, 2038, 2060
PsyI GACNNNGTC 1 cut(s) 581
PvuI CGATCG 1 cut(s) 76
PvuII CAGCTG 1 cut(s) 1631
RsaI GTAC 8 cut(s) 247, 1273, 1285, 2288, 2467, 2597, 2673, 2844
RsaNI GTAC 8 cut(s) 246, 1272, 1284, 2287, 2466, 2596, 2672, 2843
RseI CAYNNNNRTG 2 cut(s) 2303, 2582
SacI GAGCTC 1 cut(s) 195
Sau96I GGNCC 2 cut(s) 238, 918
ScaI AGTACT 1 cut(s) 247
SchI GAGTC 1 cut(s) 650
ScrFI CCNGG 9 cut(s) 127, 344, 381, 382, 567, 1461, 1559, 2264, 2351
SduI GDGCHC 4 cut(s) 195, 214, 1924, 1953
SfaNI GCATC 7 cut(s) 139, 369, 945, 1463, 1592, 1661, 1885
SfcI CTRYAG 5 cut(s) 966, 1311, 2184, 2269, 2331
Sfr274I CTCGAG 1 cut(s) 2218
SgrAI CRCCGGYG 1 cut(s) 1766
SinI GGWCC 1 cut(s) 238
SlaI CTCGAG 1 cut(s) 2218
SmaI CCCGGG 1 cut(s) 382
SmiMI CAYNNNNRTG 2 cut(s) 2303, 2582
SmlI CTYRAG 6 cut(s) 28, 1862, 2015, 2218, 2473, 2868
SmoI CTYRAG 6 cut(s) 28, 1862, 2015, 2218, 2473, 2868
SphI GCATGC 4 cut(s) 408, 1156, 1517, 1583
Sse9I AATT 9 cut(s) 434, 702, 845, 898, 1147, 1191, 1977, 2076, 2231
SsiI CCGC 5 cut(s) 1298, 1350, 1589, 1815, 2760
SstI GAGCTC 1 cut(s) 195
StyD4I CCNGG 9 cut(s) 125, 342, 379, 380, 565, 1459, 1557, 2262, 2349
StyI CCWWGG 1 cut(s) 592
TaaI ACNGT 7 cut(s) 13, 101, 321, 583, 1187, 1237, 2600
TaiI ACGT 3 cut(s) 144, 670, 2317
TaqI TCGA 1 cut(s) 2219
TasI AATT 9 cut(s) 434, 702, 845, 898, 1147, 1191, 1977, 2076, 2231
TatI WGTACW 1 cut(s) 245
TfiI GAWTC 1 cut(s) 2503
TscAI CASTG 7 cut(s) 18, 324, 586, 1054, 1240, 1447, 1495
TseFI GTSAC 5 cut(s) 225, 315, 509, 2153, 2419
Tsp45I GTSAC 5 cut(s) 225, 315, 509, 2153, 2419
TspDTI ATGAA 5 cut(s) 326, 1157, 1341, 2123, 2814
TspGWI ACGGA 3 cut(s) 361, 1562, 2072
TspMI CCCGGG 1 cut(s) 380
TspRI CASTG 7 cut(s) 18, 324, 586, 1054, 1240, 1447, 1495
Tth111I GACNNNGTC 1 cut(s) 581
Van91I CCANNNNNTGG 2 cut(s) 1049, 2255
VneI GTGCAC 1 cut(s) 1920
VpaK11BI GGWCC 1 cut(s) 238
XapI RAATTY 4 cut(s) 702, 845, 1191, 2076
XbaI TCTAGA 1 cut(s) 1195
XceI RCATGY 7 cut(s) 408, 682, 1156, 1379, 1517, 1583, 1957
XcmI CCANNNNNNNNNTGG 1 cut(s) 2588
XhoI CTCGAG 1 cut(s) 2218
XmaI CCCGGG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 1314
XmnI GAANNNNTTC 2 cut(s) 1009, 2450
ZrmI AGTACT 1 cut(s) 247
Zsp2I ATGCAT 2 cut(s) 1581, 1585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.