AT4G17610

tRNA rRNA methyltransferase (SpoU) family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008435
Physical Location & Seq
Reverse (-)
9804954 .. 9815062
10109 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G17610.2

Sequence Viewer

Length: 5487 bp
ATGGGTTCGTCAGTGATTACTTCATTGTCACTGAGCTTCAAGCAAGTTCCACCAGCAGCTATTCCTGCTTTCCTTGACTGTGTTTTAAGCTCCACAGGCGTATCTCCTTCAACTCTTTTCGAATCTCTCATTGAGCAATTCCCTTTTCGTTTAGAGGATACGGTAAATGGGGACAAGAGATTTGATTCTGATGATTGCAACCACATAGCTTCATTGGTGGCTGGGCTTTGTCATCTACTAAAGAATTTTGGCTTTGTTGCTGCGGATAATCATAATGCGTTGCAGCTATTTGTGTGGAGAGTGTTTATTCCATTGATGAAGATGGTTCGTGCATATGATTTGGATATGCTTAATAAGATTGTAGAATCGTTCTTTGATGTAGTGATCGAGACTAATGTATTGGATATGTTGGGAGTGAGCTTGGTGCCATTTTTACTAAGATCAGTTGGTTTTTCGATGGGAATGCGTCAACACGAAGAATCAGATTTCATCAAATGGGGCGACCTCTGCCTCCGTGATTCTTTGAATACAATAGATATGGATGAAAACTACATTGCACAGTTGTCTGGATCATTTCCAATTCCATTGTCGTGCCATCTTCTGAATTTGATTCTGAATGCTGCTTTTCAAAGTCACCAAGCAGCTCCGAAGGTGGAAAGTTTTGCAGCTGGTATGCTTTGGGATTTGTGTAATACGACTGAGCGGCTTTTGTCACAAAGTGTAGAACATCGTTCTTGTGCTGTTAGTTTCCTCCTCCCTGCTATTTTCAAGGCATTCTCTTCTCAGTCATCCTTGAAGATCTCACACCAAGGAAATCTGTTCATACTTTCAAGGAATGGCTTCATAAAAAGAATTTGGGAATGCTGCAAGAAGCTGTTCTCGGTTGGATCAATAGAGAGAAGGGATGCTTACAGTGTTCTGTCTTTGTGTCTTTCTTCTGGCTCTTGGACTGACGGAACTGAAAGTTTTGTTTCAGAAAAAGACGCTGTTCAATTTGACTTGAGGTCTGAACAAGAGTTTTGGGATGAAATCAAGATAGGCTTGGTTGTTGATGAGAGCTTAGTAAGGAAGCAGTCATTGCATATACTTAAATCAGTTTTATCCATTATTGAAGTGAGTGAGACCATTTCTGAGAAGAAGCCCGAAGGGAATTCAGTTAATCGGTCTATGACAAGAAAGGAGACGTGGGCAGAAAAGGAAGCCAAGTCGCTTGGCGTCGGAGAATTGTATGGCTCTGTCGACTCTGGGTTGACTAGTCAGCAGGGATGGCAAGCTTTTCTGCTCTTGTATGAGATGCTTGAGGAGTACGGCACTCATCTTGTAGAAGCAGCATGGAGTAACCAGATTGATTTATTAATCAAGTCATCTCTCAGATATGATGGAACCTTGAAATCCGACTGTAATAACTCTCATCATGGTCACATGGAAACTCCAGATGAAGAAGCTAAAATTTTTAATTGGTTAGAGGTTCTTTGGAATCGAGGCTTTCGTCATGATAACCCTCTAGTCAGGTGCACGGTCATGGAGTCATTTTTCGGTATCGAGTGGAGAAGATACAAAACATGTACACAATCAATGTCCCAAACTTTCGTTCTTGGTCCTTTCATAGAGGGTTTGAATGATCCTACACACCACAAGGATTTTGGGCTGAAAGGAATCTATACATCAAGAACAATTGAAGGTGCAGCTCAATATGTGAGTGCGTACACAAGTTGCCTCAACCCAAGGAATCGGGTTGGATTTTTGATCAACCTGGCATCTCTTGCGAAGAAACAGTCTTTTTGTAGAGCTGGATTTATGGCTCTCGTACAATGTATTGTTTCTACGGCATATGTAGTTGGAGGATACGGGGACAAGGAAATGGGGCACCTCGAAGATAAATTCTCTGGGACTGCCCAGGAATCTTCTTGTGGACATTTAAGTCAAGATGATATGACACATATACTGGATGTCTTGAAGTTTGTTGCAGAGAGCAGCAGACAGCATTTTAATCATAAATACCGTATTCGAGTTTATCAAAAAGTGTTAGAAACTGCTGCTTCTGTGGTGAACCCTTGTAATGTTCCTCTTGGAACACTATTGCAGTTTGTTTCAGCAATTCCTCGGGAGTTTACTGACCATGATGGTTTATTGCGCAAAATGATGTTAGAATGGCTCCAGGGGTGCAACAGAAAGACTTCCAACAGTTTATGTACTGATGGCACTCGTCTTCTGGCGAGCCTTTATGAGTATCTGAAAGGATTTACCAGTGATAATGTTGAGAGTTTTGATGATGAAGACTTGGAGGCATGGGATTCCCAAACAAAACGATGGGCAAGAGTATTTTTCCTCATGATTAATAAAGAAGAGCATCTCACCGATATCATAATGTTTGTGCAAAATAACGGCCTTAGTTTTTTCCAAGAGAAGAACCATTTAAAACGAGCACCTGCGAAGTTTTTAATCTTTATTCTGAGTATGCTTTTAGAGCTCCAGAACATGCAAGATGGAATTTCTGAGCTTAGTTCTTCGGTGAAAAGCAAGTCAGGCATTGGCTCTGATGAGCAAACTGGCAAGCAGATTGTTGTCGATGCTTCCAGTATTAAGAAAAAGTTTGCTGTAGTTCTCTTATCAATACTGAAGGAGCTGATTCCATTTGCTGATTCATCTTGTTCAATATTCTGGTCTCATACTACTGTGGAGAATGGTGCTCTGCCTGGTTCTGTCATAGGAAAACTAGGGGGTCCAAGTCAACGCCGGCTATCTGTTCCTACTACAACAGCTGTACTGGAAGCTGTATTGTCGGTGAAAACTATTGGGTTGATCTCGTCATACTGTGCTCAATTCACTAGTGGTGTTGGTGAACTCAAATTGGCATTGGCTTTCTTTTGGAAGTTCACCCAGCATACTATATCATCTCAAATTTGTAATTCTGAGGCCGCAGCTGAGATCTATCTTGCAGCATTTGAGGCGCTAGCTTCAGTACTGAATGCATTTGTGTCCTTGTGCTCAGCTGGAGCTTTCAACCTTTTAGAAAACGATAGTACATTGTTATCCATGGTAGATGGTGAATTTTGGTTACAAGTTTCGGTTCCGGCTTTCGTCCGTAACATTAACCATCTTCTCACAGCAGGAGTTCTGGTAAGAAGTAGAAGGGCAGTTCTGCTAAGCTGGAAGTGGCTTTGTGTGGAGTCTCTGTTATCAGTCATGCATATTCTTGATGCCAGACGTATTCCAGAGGATAGAAAGTCGTTCTTTTCAGATGATACAGTTAAAAGCATTTTTCAAGATATTGTAGAGAGCCTTGAAAATGCTGGGGAGGGTTCTGCTTTGCCTATGCTGAAATCTGTCAGACTCGCTTTGGGGATACTTGCGTCTGGGAAATCATCTTTGGATGGTTTCTCGGGTGTGGATACTCAGACGATGTGGCAGTTGGTCAAATCATGTTGGATATTGCATATCAGCTGTAAGAAAAGAAGGGTTGCACCCATTGCTGCGCTTTTGTCTTCTGTGTTGCATTCTTCTTTGTTTAGTAATAAAGATATGCACATAACTGAAGATGAGCATGGTCCGCTGAAGTGGTTCGTTGAAAAAGTACTTGAGGAAGGTCAGAAAAGTCCACGCACAATTCGCCTTGCAGCGTTGCACCTATCAGGACTCTGGCTCATGTACCCAAGGACGATAAAGTACTACATTAAAGAATTGAGGCTTCTGACTCTCTATGGTTCTGTTGCATTTGATGAAGATTTTGAAGCTGAATTGTCTGACAATAATGATGCCAGAACTGAAGTATCATTATTGGCCAAAAGCCCAGACCCTGAACTGACAGAACTTTTTATCAACACGGAGTTGTATGCACGTGTCTCTGTCGCTGGTCTGTTTCAGAAGCTGGCTAATTTGGCCTATATGGTTGAACCAGCAAGTCAAAATCAAGATTGTCAGGATGCTCTTGTGGCTGGAAAATTGTTTCTATTGGAGCTTCTTGATGCAGCGGTGCATGATAAAGACTTGGCAAAGGAGCTTTACAAGAAGTACAGTGCTATCCATAGACGGAAAATACGTGCTTGGCAGATGATATGCATTATGTCGCGATTTGTCTGCAATGACATTGTTGGCCAAGTAATGGACAGTGTGCACATATGCTTACATGTAAGTTTACAAGAACAGACTGAAAGAAACAACCTGCCTGCTGTTCGACAATACCTGGAAACATTTGCCATCAACATTTACCTGAAATTTCCAGCATTGGTGAAAGAGCAGTTAGTTCCTATACTAAAAAATTATGACAGTAAAGCTCAGCAAGCTCTCTCCTCCTATGTCTTTGTTGCAGCCAATGTTATCCTCCACGCAGAAAAGATAGCTCAACAGACACATCTAAGAGAACTTCTTCCACCAATACTACCGTTACTAACTTCTCATCATCATAGCTTACGTGGTTTCGCGCAGTTATTAGTGCATCGAGTACTCTTTAGACTGTTTCCCCCTGTAGAGTCTACATCCTCTCATACAATATCCCTGGAGAAACTTTCTTTTGAGAATTTGAAGTCTTACCTGGACAAGAATCCTGATTGTTCAAGATTGCGAGCATCAATGGAAGGATTTCTTGATGCATATGATCCCAGCACATCTGCTACTCCAGCCGGTGTTTTTGTCAATCGTGTTGAGGAAAGTGAGTTTGAATGTGTTCCAACATGCCTCATGGATAACGTGCTCTCTTTTCTGAATGATGTAAGGGAAGATCTCCGTGCCTCCATGGCAAAGGATGTAGTTACCATAAAAAATGAAGGCTTCAAGATTGATGAAGAGCCCAAGCGTCGGTTGATCATGTCCACGACAGATGAAGAAAGGCTTTCTGAGCCATCTTCGTTGGATTTCCAGAGGAAGATCACTCTTTCCAAACATGAGAAGCAAGATGCTAGTTCAACTTCTGTGCTCCGAAACGGGGAAACTTACAAGCGGCTCTTTGAAATGGAAAAAGAAGATGAGCTCGTGACTCAGCTCTTACGATCTAGAAGCATGGAGGTCGAAAGGTTGAAATCTGGTCGACAGAGTTTGATCCTTGTGGCTTCATTGGTTGATCGTATTCCTAATCTTGCTGGATTAGCCCGGACATGTGAGGTGTTTAAAGCATCAAGTCTTGCTGTTGCAGATGCAAGCATAATACACGACAAACAGTTCCAACTAATTAGTGTGACTGCTGAGAAATGGGTTCCAATCATGGAAGTCCCCGTAAACAGTTTGAAACTGTTCTTGGAGAAGAAGAAACGGGAAGGGTTCTCAATCTTGGGACTGGAACAGACAGCGAATAGCGTTTCACTTGACAAATACCAATTCCCAAAGAAGACGGTTTTGGTGCTGGGTCGTGAAAAGGAAGGCATCCCAGTGGATATAATCCATATATTGGATGCTTGTATCGAGATCCCACAACTTGGAGTTGTTAGGTCCCTCAATGTTCATGTCAGTGGTGCCATTGCCCTTTGGGAATACACTCGGCAGCAACGCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SpoU_methylase

Protein Analysis

1828

Amino Acids

205.25

Weight (kDa)

6.05

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SpoU_methylase PF00588 1670 - 1812 1.4e-26 SpoU rRNA Methylase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2437
AasI GACNNNNNNGTC 1 cut(s) 1920
Acc16I TGCGCA 1 cut(s) 2135
Acc36I ACCTGC 2 cut(s) 2437, 4170
AccB1I GGYRCC 3 cut(s) 424, 1866, 5444
AccB7I CCANNNNNTGG 3 cut(s) 4102, 5380, 5408
AccBSI CCGCTC 1 cut(s) 703
AccI GTMKAC 3 cut(s) 1239, 4472, 5023
AccII CGCG 2 cut(s) 4069, 4421
AciI CCGC 6 cut(s) 263, 703, 2919, 3551, 3971, 4936
AclWI GGATC 6 cut(s) 577, 895, 1616, 4589, 5029, 5392
AcoI YGGCCR 2 cut(s) 3780, 4093
AcuI CTGAAG 5 cut(s) 2639, 2943, 3555, 3575, 3786
AcvI CACGTG 1 cut(s) 3839
AcyI GRCGYC 1 cut(s) 1215
AdeI CACNNNGTG 1 cut(s) 719
AfiI CCNNNNNNNGG 5 cut(s) 4102, 4794, 4921, 5380, 5408
AflIII ACRYGT 4 cut(s) 1562, 3838, 4126, 5090
AhdI GACNNNNNGTC 1 cut(s) 1003
AhlI ACTAGT 2 cut(s) 1253, 2828
AjiI CACGTC 1 cut(s) 1185
AjnI CCWGG 7 cut(s) 1752, 1896, 2157, 2695, 4182, 4494, 4530
AjuI GAANNNNNNNTTGG 8 cut(s) 1575, 1607, 3353, 3385, 5213, 5245, 5312, 5344
Alw26I GTCTC 6 cut(s) 383, 1115, 1175, 2670, 3177, 3847
Alw44I GTGCAC 2 cut(s) 1513, 4112
AlwI GGATC 6 cut(s) 577, 895, 1616, 4589, 5029, 5392
AlwNI CAGNNNCTG 2 cut(s) 3797, 3868
Ama87I CYCGRG 2 cut(s) 2103, 3382
AoxI GGCC 5 cut(s) 2386, 2916, 3780, 3879, 4093
ApaLI GTGCAC 2 cut(s) 1513, 4112
ArsI GACNNNNNNTTYG 4 cut(s) 164, 196, 1760, 1792
AseI ATTAAT 2 cut(s) 1355, 2337
Asp700I GAANNNNTTC 8 cut(s) 839, 875, 2176, 3560, 4365, 4578, 4662, 5225
AspLEI GCGC 4 cut(s) 2136, 2953, 3478, 4423
AspS9I GGNCC 4 cut(s) 1598, 2723, 3548, 5421
AsuC2I CCSGG 1 cut(s) 5086
AsuHPI GGTGA 9 cut(s) 626, 2059, 2347, 2524, 2797, 2852, 2869, 3059, 4240
AsuII TTCGAA 1 cut(s) 120
AsuNHI GCTAGC 1 cut(s) 2953
AvaI CYCGRG 2 cut(s) 2103, 3382
AvaII GGWCC 4 cut(s) 1598, 2723, 3548, 5421
BaeGI GKGCMC 3 cut(s) 1517, 1869, 4116
BaeI ACNNNNGTAYC 6 cut(s) 84, 117, 3338, 3371, 3753, 3786
BalI TGGCCA 2 cut(s) 3782, 4095
BanI GGYRCC 3 cut(s) 424, 1866, 5444
BanII GRGCYC 3 cut(s) 2472, 4788, 4968
BauI CACGAG 1 cut(s) 4967
BbrPI CACGTG 1 cut(s) 3839
BbsI GAAGAC 4 cut(s) 2201, 2283, 3477, 5327
BceAI ACGGC 3 cut(s) 1324, 1842, 2401
BcgI CGANNNNNNTGC 6 cut(s) 445, 479, 4059, 4093, 4984, 5018
BciT130I CCWGG 7 cut(s) 1754, 1898, 2159, 2697, 4184, 4496, 4532
BciVI GTATCC 4 cut(s) 151, 1838, 3339, 3385
BclI TGATCA 2 cut(s) 1746, 4800
BcnI CCSGG 1 cut(s) 5086
BcoDI GTCTC 6 cut(s) 383, 1115, 1175, 2670, 3177, 3847
BcuI ACTAGT 2 cut(s) 1253, 2828
BfaI CTAG 7 cut(s) 1254, 1505, 2717, 2829, 2954, 4896, 4989
BfmI CTRYAG 2 cut(s) 2598, 4464
BfoI RGCGCY 1 cut(s) 2954
BfuAI ACCTGC 2 cut(s) 2437, 4170
BfuI GTATCC 4 cut(s) 151, 1838, 3339, 3385
BglI GCCNNNNNGGC 1 cut(s) 4733
BglII AGATCT 3 cut(s) 798, 2928, 4717
BlpI GCTNAGC 3 cut(s) 2989, 3146, 4275
BmcAI AGTACT 4 cut(s) 2964, 3576, 3668, 4443
Bme1390I CCNGG 8 cut(s) 1754, 1898, 2159, 2697, 4184, 4496, 4532, 5086
Bme18I GGWCC 4 cut(s) 1598, 2723, 3548, 5421
BmeRI GACNNNNNGTC 1 cut(s) 1003
BmeT110I CYCGRG 2 cut(s) 2103, 3382
BmgBI CACGTC 1 cut(s) 1185
BmgT120I GGNCC 4 cut(s) 1598, 2723, 3548, 5421
BmiI GGNNCC 9 cut(s) 426, 1384, 1868, 2156, 2724, 3072, 5190, 5423, 5446
BmrFI CCNGG 8 cut(s) 1754, 1898, 2159, 2697, 4184, 4496, 4532, 5086
BmrI ACTGGG 1 cut(s) 5354
BmtI GCTAGC 1 cut(s) 2957
BmuI ACTGGG 1 cut(s) 5354
BpiI GAAGAC 4 cut(s) 2201, 2283, 3477, 5327
BpmI CTGGAG 6 cut(s) 1416, 2141, 2456, 3015, 4517, 4599
Bpu1102I GCTNAGC 3 cut(s) 2989, 3146, 4275
Bpu14I TTCGAA 1 cut(s) 120
BpuEI CTTGAG 3 cut(s) 1021, 1319, 3599
BpuMI CCSGG 1 cut(s) 5086
BsaAI YACGTR 3 cut(s) 3839, 4040, 4412
BsaHI GRCGYC 1 cut(s) 1215
BsaI GGTCTC 2 cut(s) 1115, 2670
BsaJI CCNNGG 9 cut(s) 808, 1724, 1896, 2102, 2158, 3036, 3653, 4494, 4731
BsaXI ACNNNNNCTCC 4 cut(s) 3105, 3135, 3161, 3191
Bsc4I CCNNNNNNNGG 5 cut(s) 4102, 4794, 4921, 5380, 5408
Bse118I RCCGGY 2 cut(s) 2736, 4619
Bse1I ACTGG 7 cut(s) 1950, 2247, 2554, 2577, 2772, 5274, 5360
Bse3DI GCAATG 5 cut(s) 552, 1076, 3468, 4087, 5448
BseBI CCWGG 7 cut(s) 1754, 1898, 2159, 2697, 4184, 4496, 4532
BseDI CCNNGG 9 cut(s) 808, 1724, 1896, 2102, 2158, 3036, 3653, 4494, 4731
BseLI CCNNNNNNNGG 5 cut(s) 4102, 4794, 4921, 5380, 5408
BseMI GCAATG 5 cut(s) 552, 1076, 3468, 4087, 5448
BseNI ACTGG 7 cut(s) 1950, 2247, 2554, 2577, 2772, 5274, 5360
BseRI GAGGAG 3 cut(s) 743, 1316, 4279
BseSI GKGCMC 3 cut(s) 1517, 1869, 4116
BseYI CCCAGC 5 cut(s) 221, 2880, 3293, 4598, 5335
BsgI GTGCAG 1 cut(s) 1704
Bsh1236I CGCG 2 cut(s) 4069, 4421
BshFI GGCC 5 cut(s) 2388, 2918, 3782, 3881, 4095
BshNI GGYRCC 3 cut(s) 424, 1866, 5444
BsiHKCI CYCGRG 2 cut(s) 2103, 3382
BsiSI CCGG 4 cut(s) 2737, 3074, 4620, 5086
BslFI GGGAC 7 cut(s) 185, 1564, 1865, 1903, 5189, 5280, 5407
BslI CCNNNNNNNGG 5 cut(s) 4102, 4794, 4921, 5380, 5408
BsmAI GTCTC 6 cut(s) 383, 1115, 1175, 2670, 3177, 3847
BsmBI CGTCTC 1 cut(s) 1175
BsmFI GGGAC 7 cut(s) 185, 1564, 1865, 1903, 5189, 5280, 5407
BsmI GAATGC 6 cut(s) 468, 622, 773, 866, 2974, 3496
BsnI GGCC 5 cut(s) 2388, 2918, 3782, 3881, 4095
Bso31I GGTCTC 2 cut(s) 1115, 2670
BsoBI CYCGRG 2 cut(s) 2103, 3382
Bsp119I TTCGAA 1 cut(s) 120
Bsp1407I TGTACA 1 cut(s) 1565
Bsp1720I GCTNAGC 3 cut(s) 2989, 3146, 4275
Bsp19I CCATGG 2 cut(s) 3036, 4731
Bsp68I TCGCGA 1 cut(s) 4069
BspACI CCGC 6 cut(s) 263, 703, 2919, 3551, 3971, 4936
BspANI GGCC 5 cut(s) 2388, 2918, 3782, 3881, 4095
BspFNI CGCG 2 cut(s) 4069, 4421
BspHI TCATGA 2 cut(s) 1492, 2331
BspLI GGNNCC 9 cut(s) 426, 1384, 1868, 2156, 2724, 3072, 5190, 5423, 5446
BspMI ACCTGC 2 cut(s) 2437, 4170
BspOI GCTAGC 1 cut(s) 2957
BspPI GGATC 6 cut(s) 577, 895, 1616, 4589, 5029, 5392
BspQI GCTCTTC 2 cut(s) 2340, 4776
BspT104I TTCGAA 1 cut(s) 120
BspT107I GGYRCC 3 cut(s) 424, 1866, 5444
BspTNI GGTCTC 2 cut(s) 1115, 2670
BsrBI CCGCTC 1 cut(s) 703
BsrDI GCAATG 5 cut(s) 552, 1076, 3468, 4087, 5448
BsrFI RCCGGY 2 cut(s) 2736, 4619
BsrGI TGTACA 1 cut(s) 1565
BsrI ACTGG 7 cut(s) 1950, 2247, 2554, 2577, 2772, 5274, 5360
BssAI RCCGGY 2 cut(s) 2736, 4619
BssECI CCNNGG 9 cut(s) 808, 1724, 1896, 2102, 2158, 3036, 3653, 4494, 4731
BssNI GRCGYC 1 cut(s) 1215
BssSI CACGAG 1 cut(s) 4967
BssT1I CCWWGG 5 cut(s) 808, 1724, 3036, 3653, 4731
Bst2BI CACGAG 1 cut(s) 4967
Bst2UI CCWGG 7 cut(s) 1754, 1898, 2159, 2697, 4184, 4496, 4532
Bst6I CTCTTC 3 cut(s) 784, 2340, 4776
BstACI GRCGYC 1 cut(s) 1215
BstAPI GCANNNNNTGC 3 cut(s) 1078, 1763, 3470
BstAUI TGTACA 1 cut(s) 1565
BstBAI YACGTR 3 cut(s) 3839, 4040, 4412
BstBI TTCGAA 1 cut(s) 120
BstDSI CCRYGG 2 cut(s) 3036, 4731
BstFNI CGCG 2 cut(s) 4069, 4421
BstH2I RGCGCY 1 cut(s) 2954
BstHHI GCGC 4 cut(s) 2136, 2953, 3478, 4423
BstMAI GTCTC 6 cut(s) 383, 1115, 1175, 2670, 3177, 3847
BstNI CCWGG 7 cut(s) 1754, 1898, 2159, 2697, 4184, 4496, 4532
BstNSI RCATGY 5 cut(s) 1566, 2482, 4130, 4674, 5094
BstSCI CCNGG 8 cut(s) 1752, 1896, 2157, 2695, 4182, 4494, 4530, 5084
BstSFI CTRYAG 2 cut(s) 2598, 4464
BstSLI GKGCMC 3 cut(s) 1517, 1869, 4116
BstUI CGCG 2 cut(s) 4069, 4421
BstV2I GAAGAC 4 cut(s) 2201, 2283, 3477, 5327
BstX2I RGATCY 4 cut(s) 798, 2928, 4717, 5397
BstYI RGATCY 4 cut(s) 798, 2928, 4717, 5397
BsuI GTATCC 4 cut(s) 151, 1838, 3339, 3385
BsuRI GGCC 5 cut(s) 2388, 2918, 3782, 3881, 4095
BtgI CCRYGG 2 cut(s) 3036, 4731
BtrI CACGTC 1 cut(s) 1185
BtsIMutI CAGTG 8 cut(s) 18, 29, 919, 2254, 4021, 4114, 5367, 5446
BtuMI TCGCGA 1 cut(s) 4069
BveI ACCTGC 2 cut(s) 2437, 4170
CaiI CAGNNNCTG 2 cut(s) 3797, 3868
CciI TCATGA 2 cut(s) 1492, 2331
CfoI GCGC 4 cut(s) 2136, 2953, 3478, 4423
Cfr10I RCCGGY 2 cut(s) 2736, 4619
Cfr13I GGNCC 4 cut(s) 1598, 2723, 3548, 5421
CseI GACGC 5 cut(s) 455, 992, 1204, 3342, 4781
DraI TTTAAA 2 cut(s) 2418, 5104
DraIII CACNNNGTG 1 cut(s) 719
DrdI GACNNNNNNGTC 1 cut(s) 1920
DriI GACNNNNNGTC 1 cut(s) 1003
DseDI GACNNNNNNGTC 1 cut(s) 1920
EaeI YGGCCR 2 cut(s) 3780, 4093
Eam1104I CTCTTC 3 cut(s) 784, 2340, 4776
Eam1105I GACNNNNNGTC 1 cut(s) 1003
EarI CTCTTC 3 cut(s) 784, 2340, 4776
Ecl136II GAGCTC 2 cut(s) 2470, 4966
Eco130I CCWWGG 5 cut(s) 808, 1724, 3036, 3653, 4731
Eco24I GRGCYC 3 cut(s) 2472, 4788, 4968
Eco31I GGTCTC 2 cut(s) 1115, 2670
Eco32I GATATC 1 cut(s) 2362
Eco47I GGWCC 4 cut(s) 1598, 2723, 3548, 5421
Eco53kI GAGCTC 2 cut(s) 2470, 4966
Eco57I CTGAAG 5 cut(s) 2639, 2943, 3555, 3575, 3786
Eco72I CACGTG 1 cut(s) 3839
Eco88I CYCGRG 2 cut(s) 2103, 3382
EcoICRI GAGCTC 2 cut(s) 2470, 4966
EcoO109I RGGNCCY 1 cut(s) 5421
EcoRI GAATTC 1 cut(s) 1150
EcoRII CCWGG 7 cut(s) 1752, 1896, 2157, 2695, 4182, 4494, 4530
EcoRV GATATC 1 cut(s) 2362
EcoT14I CCWWGG 5 cut(s) 808, 1724, 3036, 3653, 4731
EcoT22I ATGCAT 4 cut(s) 2974, 3192, 4061, 4591
EcoT38I GRGCYC 3 cut(s) 2472, 4788, 4968
ErhI CCWWGG 5 cut(s) 808, 1724, 3036, 3653, 4731
Esp3I CGTCTC 1 cut(s) 1175
FaqI GGGAC 7 cut(s) 185, 1564, 1865, 1903, 5189, 5280, 5407
FauNDI CATATG 4 cut(s) 334, 1831, 4118, 4591
FbaI TGATCA 2 cut(s) 1746, 4800
FblI GTMKAC 3 cut(s) 1239, 4472, 5023
FriOI GRGCYC 3 cut(s) 2472, 4788, 4968
FspBI CTAG 7 cut(s) 1254, 1505, 2717, 2829, 2954, 4896, 4989
FspI TGCGCA 1 cut(s) 2135
GlaI GCGC 4 cut(s) 2135, 2952, 3477, 4422
GsaI CCCAGC 5 cut(s) 225, 2884, 3297, 4602, 5339
GsuI CTGGAG 6 cut(s) 1416, 2141, 2456, 3015, 4517, 4599
HaeII RGCGCY 1 cut(s) 2954
HaeIII GGCC 5 cut(s) 2388, 2918, 3782, 3881, 4095
HapII CCGG 4 cut(s) 2737, 3074, 4620, 5086
HgaI GACGC 5 cut(s) 455, 992, 1204, 3342, 4781
HhaI GCGC 4 cut(s) 2136, 2953, 3478, 4423
Hin1I GRCGYC 1 cut(s) 1215
Hin6I GCGC 4 cut(s) 2134, 2951, 3476, 4421
HinP1I GCGC 4 cut(s) 2134, 2951, 3476, 4421
HincII GTYRAC 5 cut(s) 470, 1240, 1251, 2732, 5024
HindII GTYRAC 5 cut(s) 470, 1240, 1251, 2732, 5024
HindIII AAGCTT 1 cut(s) 1272
HpaII CCGG 4 cut(s) 2737, 3074, 4620, 5086
HphI GGTGA 9 cut(s) 626, 2059, 2347, 2524, 2797, 2852, 2869, 3059, 4240
Hpy99I CGWCG 2 cut(s) 1220, 4797
HpyCH4IV ACGT 6 cut(s) 1184, 3208, 3838, 4039, 4411, 4686
HpySE526I ACGT 6 cut(s) 1184, 3208, 3838, 4039, 4411, 4686
Hsp92I GRCGYC 1 cut(s) 1215
HspAI GCGC 4 cut(s) 2134, 2951, 3476, 4421
KroI GCCGGC 1 cut(s) 2736
KroNI GCCGGC 1 cut(s) 2738
Ksp22I TGATCA 2 cut(s) 1746, 4800
LguI GCTCTTC 2 cut(s) 2340, 4776
LmnI GCTCC 9 cut(s) 95, 649, 2160, 2475, 2623, 2996, 3955, 3997, 4917
MaeI CTAG 7 cut(s) 1254, 1505, 2717, 2829, 2954, 4896, 4989
MaeII ACGT 6 cut(s) 1184, 3208, 3838, 4039, 4411, 4686
MbiI CCGCTC 1 cut(s) 703
MfeI CAATTG 1 cut(s) 1674
MflI RGATCY 4 cut(s) 798, 2928, 4717, 5397
MlsI TGGCCA 2 cut(s) 3782, 4095
MluNI TGGCCA 2 cut(s) 3782, 4095
MlyI GAGTC 8 cut(s) 1235, 1535, 3179, 3327, 3630, 3688, 4478, 4966
Mox20I TGGCCA 2 cut(s) 3782, 4095
Mph1103I ATGCAT 4 cut(s) 2974, 3192, 4061, 4591
MroNI GCCGGC 1 cut(s) 2736
MroXI GAANNNNTTC 8 cut(s) 839, 875, 2176, 3560, 4365, 4578, 4662, 5225
MscI TGGCCA 2 cut(s) 3782, 4095
MslI CAYNNNNRTG 4 cut(s) 589, 1520, 5360, 5439
Msp20I TGGCCA 2 cut(s) 3782, 4095
MspA1I CMGCKG 7 cut(s) 668, 2762, 2924, 2993, 3444, 3553, 3971
MspI CCGG 4 cut(s) 2737, 3074, 4620, 5086
MspR9I CCNGG 8 cut(s) 1754, 1898, 2159, 2697, 4184, 4496, 4532, 5086
MunI CAATTG 1 cut(s) 1674
Mva1269I GAATGC 6 cut(s) 468, 622, 773, 866, 2974, 3496
MvaI CCWGG 7 cut(s) 1754, 1898, 2159, 2697, 4184, 4496, 4532
MvnI CGCG 2 cut(s) 4069, 4421
NaeI GCCGGC 1 cut(s) 2738
NciI CCSGG 1 cut(s) 5086
NcoI CCATGG 2 cut(s) 3036, 4731
NdeI CATATG 4 cut(s) 334, 1831, 4118, 4591
NgoMIV GCCGGC 1 cut(s) 2736
NheI GCTAGC 1 cut(s) 2953
NlaIV GGNNCC 9 cut(s) 426, 1384, 1868, 2156, 2724, 3072, 5190, 5423, 5446
NmeAIII GCCGAG 1 cut(s) 5449
NmuCI GTSAC 6 cut(s) 27, 632, 711, 1418, 4969, 5170
NruI TCGCGA 1 cut(s) 4069
NsbI TGCGCA 1 cut(s) 2135
NsiI ATGCAT 4 cut(s) 2974, 3192, 4061, 4591
NspI RCATGY 5 cut(s) 1566, 2482, 4130, 4674, 5094
NspV TTCGAA 1 cut(s) 120
PagI TCATGA 2 cut(s) 1492, 2331
PaqCI CACCTGC 1 cut(s) 2437
PciI ACATGT 3 cut(s) 1562, 4126, 5090
PciSI GCTCTTC 2 cut(s) 2340, 4776
PcsI WCGNNNNNNNCGW 2 cut(s) 1486, 4036
PctI GAATGC 6 cut(s) 468, 622, 773, 866, 2974, 3496
PdiI GCCGGC 1 cut(s) 2738
PdmI GAANNNNTTC 8 cut(s) 839, 875, 2176, 3560, 4365, 4578, 4662, 5225
PflMI CCANNNNNTGG 3 cut(s) 4102, 5380, 5408
PleI GAGTC 8 cut(s) 1235, 1534, 3178, 3327, 3630, 3688, 4477, 4966
PmaCI CACGTG 1 cut(s) 3839
PmlI CACGTG 1 cut(s) 3839
PpsI GAGTC 8 cut(s) 1235, 1534, 3178, 3327, 3630, 3688, 4477, 4966
Ppu21I YACGTR 3 cut(s) 3839, 4040, 4412
PpuMI RGGWCCY 1 cut(s) 5421
PscI ACATGT 3 cut(s) 1562, 4126, 5090
PshBI ATTAAT 2 cut(s) 1355, 2337
Psp124BI GAGCTC 2 cut(s) 2472, 4968
Psp5II RGGWCCY 1 cut(s) 5421
Psp6I CCWGG 7 cut(s) 1752, 1896, 2157, 2695, 4182, 4494, 4530
PspCI CACGTG 1 cut(s) 3839
PspFI CCCAGC 5 cut(s) 221, 2880, 3293, 4598, 5335
PspGI CCWGG 7 cut(s) 1752, 1896, 2157, 2695, 4182, 4494, 4530
PspN4I GGNNCC 9 cut(s) 426, 1384, 1868, 2156, 2724, 3072, 5190, 5423, 5446
PspPI GGNCC 4 cut(s) 1598, 2723, 3548, 5421
PspPPI RGGWCCY 1 cut(s) 5421
PstNI CAGNNNCTG 2 cut(s) 3797, 3868
PsuI RGATCY 4 cut(s) 798, 2928, 4717, 5397
PvuII CAGCTG 5 cut(s) 668, 2762, 2924, 2993, 3444
RruI TCGCGA 1 cut(s) 4069
RseI CAYNNNNRTG 4 cut(s) 589, 1520, 5360, 5439
SacI GAGCTC 2 cut(s) 2472, 4968
SalI GTCGAC 2 cut(s) 1238, 5022
SapI GCTCTTC 2 cut(s) 2340, 4776
Sau96I GGNCC 4 cut(s) 1598, 2723, 3548, 5421
ScaI AGTACT 4 cut(s) 2964, 3576, 3668, 4443
SchI GAGTC 8 cut(s) 1235, 1535, 3179, 3327, 3630, 3688, 4478, 4966
ScrFI CCNGG 8 cut(s) 1754, 1898, 2159, 2697, 4184, 4496, 4532, 5086
SfcI CTRYAG 2 cut(s) 2598, 4464
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 4 cut(s) 1598, 2723, 3548, 5421
SmiMI CAYNNNNRTG 4 cut(s) 589, 1520, 5360, 5439
SmlI CTYRAG 3 cut(s) 1000, 1298, 3578
SmoI CTYRAG 3 cut(s) 1000, 1298, 3578
SpeI ACTAGT 2 cut(s) 1253, 2828
SsiI CCGC 6 cut(s) 263, 703, 2919, 3551, 3971, 4936
SspI AATATT 1 cut(s) 2658
SspMI CTAG 7 cut(s) 1254, 1505, 2717, 2829, 2954, 4896, 4989
SstI GAGCTC 2 cut(s) 2472, 4968
StyD4I CCNGG 8 cut(s) 1752, 1896, 2157, 2695, 4182, 4494, 4530, 5084
StyI CCWWGG 5 cut(s) 808, 1724, 3036, 3653, 4731
TaiI ACGT 6 cut(s) 1187, 3211, 3841, 4042, 4414, 4689
TaqII GACCGA 1 cut(s) 1152
TatI WGTACW 9 cut(s) 1565, 2192, 2764, 2962, 3023, 3574, 3666, 4011, 4441
TauI GCSGC 3 cut(s) 706, 2921, 4939
TscAI CASTG 8 cut(s) 18, 36, 919, 2254, 4021, 4114, 5367, 5446
TseFI GTSAC 6 cut(s) 27, 632, 711, 1418, 4969, 5170
Tsp45I GTSAC 6 cut(s) 27, 632, 711, 1418, 4969, 5170
TspGWI ACGGA 6 cut(s) 503, 969, 3074, 3839, 4045, 4712
TspRI CASTG 8 cut(s) 18, 36, 919, 2254, 4021, 4114, 5367, 5446
Van91I CCANNNNNTGG 3 cut(s) 4102, 5380, 5408
VneI GTGCAC 2 cut(s) 1513, 4112
VpaK11BI GGWCC 4 cut(s) 1598, 2723, 3548, 5421
VspI ATTAAT 2 cut(s) 1355, 2337
XbaI TCTAGA 1 cut(s) 4988
XceI RCATGY 5 cut(s) 1566, 2482, 4130, 4674, 5094
XcmI CCANNNNNNNNNTGG 1 cut(s) 1640
XmiI GTMKAC 3 cut(s) 1239, 4472, 5023
XmnI GAANNNNTTC 8 cut(s) 839, 875, 2176, 3560, 4365, 4578, 4662, 5225
XspI CTAG 7 cut(s) 1254, 1505, 2717, 2829, 2954, 4896, 4989
ZrmI AGTACT 4 cut(s) 2964, 3576, 3668, 4443
Zsp2I ATGCAT 4 cut(s) 2974, 3192, 4061, 4591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.