Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0008435

Physical Location & Seq

Genomic Coordinates Reverse (-)

21253838 .. 21274911

Gene Structure 21074 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G019240.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5508 bp

ATGTCTACACAAGAAACCCACTCTATGGCTACTCTTATCACTTCACTCTCCACCAGCTTCAGCCAAGTCCCTCCGGCGGCTGTTCCGGCCGTCTTGGATTGCATTTTGGCCTCTACCGGCGTATCTCGGTCGTCGCTATTCGCTTCGCTTCTCGACACTTTCACCAAGCTCTCTGAGGGTGTCATGAAAAATGACCAGAAGTTGGACTCCCATGACTGTAACTATCTGGCATCATTGGTTGGGGCACTTTGTCACCTTCTAAAGAAATTTGACACTAATCTTGGTGCTTTGGAATCATTTATATGGAAATGTTTTCTTCCATTGATAAAGATGGCACACATGTTCAGCCGCAATATGCTTAACCAGATTACAGAATCGTTTATTAATGCTGTGACTGAGACAAACACCTGGGTAGTTGTGGAGGCAACACTGGTGCCAATTTTTCTAACATTAGTTGCCAGTTCTATTGGTATGCCTGAAAAGGAAGTATCAGATACTTTTCAGTTCATGTTGGGATCTAATGACCGGGTAAATCATATGGAAATGAGTACAAAAAACATCCTTTCGCCACCTGGATCACCTTCGCTGCCAATTTCATGCCACATCCTAACCTTAATGCTTGATGCTGTCCTTCAAAGCAAGAAAATAGCTGCCTCCTCAGAATCAATAATTGCAAATGGGTGTTTTGATGATAATTTTGCTGGTAAATTGACTTGGGGTCTTTGTAGCTTGACTGAGCAATTGCTTTTACAAAATTTGGAACACCGGTCCTGTGCTACTGCCTTTCTTCTCCCAATCATTTTCAAAGCATTTTCTTCTTATTGCTCTTTTCAAATCTCAATAGATGGGCAGACCTTTACACTTTCAAGGAACAACTTCTTTATGAAAATTTGGAAGTGCTGTGGGACACTGTTTTCTCTTGGAACCGTGGAGAGAAGAGATGCTTTTGGCGTACTCTCCTTGTACCTGTCTTTTTTTCCTTTTACAGAAGAAAGTGGAGATACCGACATGAGTGATAAAGTGGAATGTTTTGATATAAGAGCTGAAACCAAATTTTGGGACGAGATTAAAAGAGGCCTGGTTGATAAGGAGAGCTTAGTGAGGAAGCAATCGTTACACATATTAAAGATAGCACTACGCATAAATGGAGCAGTCCAATCCTCCGGTGTTTTAGAGGATATATCGGCTGAAAATAATTCAATGCCTCACAGCATGACAAAGAGGGAGCTGTGGGCTGATAAGGAAGCTAAATCTCTGGGTGTGGGGAAAATATGTAGCCAAGTTGAGTCTTCTCCGGACAGCCAGAACAAGTGGGAGGCATTTGTATTGCTGTATGAAATGCTTGAAGAGTATGGAACTCACCTCGTTGAAGCAGCTTGGAATTACCAGGTATCCTTGTTACTTCGAAACTCTAGCTCAGATGCTAATAATAAAACCTCTATCAGTGGAGCAGTTCATCAAAACCAGATCGAAAGTCCAGGAGAAATCTTTAACTGGTTAGCAATTTTGTGGGAACGGGGTTTTAATCATGACAATCCTCAAGTTAGGTGCTTGATCATGCAGTCATTTTTAGGGATTGATTGGAAGAATTACGGCACACATGCAAGATCGGTGCCTGGATATTTTGTTCTTGGTTCATTTATACAAGGGCTAAATGACACTGTGCATCACAAAGAATTTGGTGTAAAAGGAATATACTCTTCAATGACAATAGAAGGTGCAGCTAACTTTCTAAGCGAATATACAAGTTATCTGAATGTGAGGAAGTGCATTATGTTTTTGAGTGACTTAGCATCTGCTGCTAAGCGGCAACCTCTTGGTAGAGTTGGATTGATGTGCCTTGCTGAATGCATTTCTTCTGCTGCTTGTCTAGTCAGAAAGAATAATAGTGAAATTGATGCACAGTGCTTTGAAGATGGAGTTCATGACATGATCAAAGTGGGAAAGTCTCCTCACAATGACAAAATTGTTTTGCTTGATGCCTTGAGATTTATTATTGAGAGCAGCAAACAACATTTCAATTCTAATTATCGTCTTCGAGTTTGTGAGAAAGTCCTTGACTCTGCTGCTTCAGTGGTGTGTACATGTGATCTGCCTCTTGATGTACTTTTGCACTTCATTTCAACATTTCCATGGGAGTTCACTGATCACGGCGGTCCCTTAAGAGAGAAAATACGACAATGGCTTATGGGGTGTGGTAAGCGTTGTAGTGTCAACTGCTGCAGTTCTGAGATGAAGCTCCTGAAGAGTCTTTATGACTTTCCAAATAGGTTTACGAGCCATTGCAATGATGATGCTTCAACCACTTTTGACGATGAAGATTTGGATGCATGGGAATTTGAAGCGAAGCGGTGGGCAAGAGTGCTCTTTATTGCATGCAAGGAGGAATATCAATTGTTACCCATACTGATGTTTATTCAAAATCACGGTAGTGATTTCTGCAAACAAAACAATAAGATGGACCTATTTCCTGTGAAGTTTTTCATCCTCATCTTGAGTCTAGTTCTGGAGCTTCAGATGATGCAAGAAAGAGTTGCCAAACATGGTTCTACGCTTAGAACCAAATCAGAGTTTTTTGTGCCAGAATCAATGAATGAATTTAGTCATGCAGATGTTCCTAATTTATATCAAAAGTTCACGGACATTTTTCTTCTCATATTGGAGGAGTTGGTCTCTTTTGCAAACTTGTCTTGTTCCATATTCTCAAATAACAATACCACTACGATGAGAGATGCAACCCTTCCTGGTTCTGTAACCGGAAAACTCGGAGGACCTAGTCAACGCCGGTTGTCATCTTCTACCACTACTGCTGTATTGCAAGCAATTATGTCCATGAAAGCTGTTGCAACTATCTCCTCATTCTGTGCACGAGTTAAAACTGATGTTTCACTTGATCTTCCTATTAATTTTTTGTGGGATTTCTACTGGAAGACTATTTCGTCTCCAGCTTGTGATTCAGAGACTGGAGCAGAAATATGTCTTGCAGCATATGAAGCATTAGCTCCTACTCTCACAGCACTAGTGTCTGTGTGTTCTCCTCAAACTTTGGATATACTTAAGAAAAATGACAGCTCATTACTATCAGATGGCAAGCCTCTGTTGGATTCTTTGGTGCTTTCTTTTCTTCAGAACATAAATAAGCTCCTTACAGTTGGAGTATTTGTACGATCTCGACGTGCAATTTTAATGAATTGGAAGTGGATTTGCCTGGAATCTCTACTGTCAATTCCTTGCTATGCTCTTAGGAATGGGCTTCTTCTAGAGGCAAATAATTTTGTCTTCTCAGGTGCTGCTCTCCGATGTATTTTTTCTGATATTCTTGAGAGCCTTGAGAATGCTGGGGAAGGTTCTGTTTTACCGATGCTCAGATCTGTCCGATTAGTTTTGGACTTATTTGCCCAAGGAAAGTCTGGTTCACTTGTCTGCTTATGTGATGGGGTGGATTCCCAGATGCTGTGGCATTTGGTTCATTCTTCTTGGATATTGCATGTCAGTTGCAACAAGCGGAAGGTTGCACCTATTGCTGCACTTTTGTCTTCCGTTCTCCATTCATCTCTATTTAGTGATGAGAGCATGCACATAATTGATGGTGCACCTGGGCCTCTGAAGTGGTTTGTTGAGAAAATTCTAGAGGAAGGCACGAAAAGTCCCCGTACAATTCGTCTTGCTGCATTACACTTAACAGGGCTGTGGCTATCATATCCTAGGATTATAAAGTACTATGTGAAGGAGCTGAAGCTGCTATCTCTACATGGTTCCGTTGCATTTGATGAGGACTTCGAAGGTGAATTAGCTGATAATCGTGATACCAGGGCTGAAGTTTCTTTGTTGGCAAAAGGCCCTGATCCTGAGCTGACCAAAGCATTTATTAATACAGAGTTGTATGCACGAGCATCTGTTGCTGTTTTGTTTTATAAGCTAGCAGACATGGCTGTCATGGTGGGATCAACTAATGAAAATGCAGATTGCCATGCAGCTTTGGAATCTGGAAAAATGTTTTTGCTTGAGCTTCTTGATTCCGCGGTAAACGAAAAGGATCTTGCAAAAGAGTTGTATAAGAAGTATAGTGCAAAGAAGGTACGGGCATGGCAAATGATATGTATTTTGTCGCATTTCGTCTGTCAAGATATAGTTTCAGAAGTTGCACGTTGCTTGCACACATCCCTCTATAGGAACAACTTACCTGCAGTTCGTCAATATTTGGAAACATTTGCAATTAATATATACTTAAAGTTTCCATCACTGGTTGGGGAGCAACTAGTTCCTATATTAAGAGATTATGACATGAGGCCTCAGGCTCTCTCTTCTTACGTATTTATAGCAGCTAACGTCATCCTCCATGCATCTCAATCTGTTCAACACCGACATCTGAATGAATTACTTCCTCCTATAGTTCCACTATTAACCTCACATCACCACAGTCTACGTGGTTTCACCCAGTTGCTGGTTTACCAAGTTCTTTGCAAATTGTTCCCTCCATTGGATTCTAAAGCCTCTGAAACTATGACTTTAGAGAAGAGGTGTTTTGAGGATCTCAAATCGTACCTCGCAAAGAATTCTGATTGTACGCGCTTACGACAATCAATGGAAGGATATCTTGATGCCTACAGTCCCCATAGCTCGGTCACTCCTGCAGGCGTTTTCATCAACCGGGTTGAGGACGTCGAGTTTGAATGTGTACCAAGATCCCTTATGGAGCAAGTGCTCAACTTTTTGAATGATGTGAGGGAAGACCTTCGAAATTCAATGGCAAAGGATGTTATGACTATTAAGAATGAGAGTTTGAGATCTGATGGAGATCAAGACCACATGGCGATACTGTCTAATGCTAATGAAAGAAAATTGAGTACCCAGCTGCCTAAAGATACCTCACTAGATTTTCAGAAGAAGATTACTCTGTCTAAACACGAGAAGCAAGACATAAACGTTAATTCTCACCAAGAAACTTACAAACAACTTCAGGAAGTAGAAAAGGACGATAAGCTTCTTGCTCAGGTGCTGCAGTCTAGAACTCTAGGAATGGAAAGAGAGAGATCAAGCAGACAACATCTTATCCTTGTAGCATCTCTGCTTGATCGGATACCTAACCTAGCTGGCCTTGCACGGACTTGTGAGGTATTTAAAGCATCAAGTTTGGCCATTGCAGATACGAACGTGTTACATGACAAACAGTTCCAACTCATCAGTGTGACTGCAGAGAAGTGGGTTCCTATCATCGAAGTCCCAGTGAATAGTTTGAAGGTGTTCCTTCAGAAAAAGAAACGAGAAGGCTTCTCAATTTTAGGACTAGAGCAGACTGCAAATAGTATACCTCTTGATCAATATATATTTCCAAAAGACACAGTACTGGTTCTTGGGCGAGAGAAGGAAGGTATACCAGTTGATATAATACATATTCTGGATGCTTGCCTTGAGATTCCACAGTTGGGAGTTGTTAGATCTCTCAATGTTCATGTCAGTGGTGCCATTGCTCTGTGGGAGTATACTAGACAGCAGAGATCCCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000451 GO:0000453 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006355 GO:0006357 GO:0006364 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008173 GO:0008175 GO:0008649 GO:0008757 GO:0009451 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016072 GO:0016423 GO:0016435 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0022613 GO:0030488 GO:0031167 GO:0031323 GO:0031326 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070039 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:0140102 GO:1901360 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SpoU_methylase

Protein Analysis

1835

Amino Acids

205.7

Weight (kDa)

6.06

Isoelectric Point (pI)

46.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SpoU_methylase	PF00588	1684 - 1826	4.9e-25	SpoU rRNA Methylase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008435)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17610 AT4G17610 AT4G17610
fragaria_vesca	FvH4_5g20370 FvH4_5g20370 FvH4_5g20370 FvH4_5g20370 FvH4_5g20370
malus_domestica	MD04G1055600.v1.1
prunus_persica	Prupe.5G055400_v2.0.a1 Prupe.5G055400_v2.0.a1
pyrus_communis	pycom04g04890
rosa_chinensis	RchiOBHm_Chr7g0205631
rosa_laevigata	RLG00000003369
rosa_multiflora	Rmu_ssc0000482.1_g000025
rosa_roxburghii	Rroxscaffold_3G00252130
rosa_rugosa	Rorug07G0092300
rosa_samantha	Rh7AG223100 Rh7AG230100 Rh7BG218100 Rh7CG236300 Rh7DG230000
rosa_wichuraiana	Rw7G019240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	3713, 3915
AasI	GACNNNNNNGTC	2 cut(s)	2938, 3932
AatII	GACGTC	1 cut(s)	4665
Acc36I	ACCTGC	1 cut(s)	4192
AccB1I	GGYRCC	3 cut(s)	433, 1612, 5462
AccB7I	CCANNNNNTGG	4 cut(s)	25, 202, 1056, 4444
AccI	GTMKAC	5 cut(s)	5, 4423, 5308, 5374, 5483
AccII	CGCG	2 cut(s)	4022, 4570
AccIII	TCCGGA	1 cut(s)	1294
AciI	CCGC	8 cut(s)	77, 349, 1807, 2154, 2350, 3505, 4020, 4022
AclI	AACGTT	1 cut(s)	4926
AclWI	GGATC	8 cut(s)	523, 583, 3839, 3952, 4044, 4539, 4680, 5493
AcoI	YGGCCR	2 cut(s)	87, 5136
AcyI	GRCGYC	1 cut(s)	4662
AflII	CTTAAG	2 cut(s)	2161, 3056
AflIII	ACRYGT	3 cut(s)	339, 2084, 5154
AgeI	ACCGGT	1 cut(s)	765
AhdI	GACNNNNNGTC	1 cut(s)	717
AhlI	ACTAGT	2 cut(s)	3019, 4258
AjiI	CACGTC	1 cut(s)	3176
AleI	CACNNNNGTG	1 cut(s)	2430
AloI	GAACNNNNNNTCC	2 cut(s)	1611, 1643
Alw21I	GWGCWC	4 cut(s)	2367, 2869, 3595, 4707
Alw26I	GTCTC	5 cut(s)	392, 1953, 2677, 2946, 2954
Alw44I	GTGCAC	2 cut(s)	2865, 3591
AlwI	GGATC	8 cut(s)	523, 583, 3839, 3952, 4044, 4539, 4680, 5493
AlwNI	CAGNNNCTG	5 cut(s)	2963, 3290, 3454, 4444, 4999
Aor13HI	TCCGGA	1 cut(s)	1294
AoxI	GGCC	8 cut(s)	87, 108, 1075, 3599, 3838, 4289, 5095, 5136
ApaLI	GTGCAC	2 cut(s)	2865, 3591
ArsI	GACNNNNNNTTYG	2 cut(s)	4652, 4684
AseI	ATTAAT	4 cut(s)	384, 2904, 3870, 4218
AsiGI	ACCGGT	1 cut(s)	765
Asp700I	GAANNNNTTC	5 cut(s)	875, 2480, 4380, 4734, 5243
AspA2I	CCTAGG	1 cut(s)	3704
AspLEI	GCGC	1 cut(s)	4572
AspS9I	GGNCC	6 cut(s)	768, 2156, 2461, 2771, 3599, 3839
AsuC2I	CCSGG	2 cut(s)	527, 4652
AsuHPI	GGTGA	8 cut(s)	154, 245, 570, 1352, 3797, 4406, 4426, 4928
AsuII	TTCGAA	3 cut(s)	1405, 3780, 4738
AsuNHI	GCTAGC	1 cut(s)	3919
AvaII	GGWCC	4 cut(s)	768, 2156, 2461, 2771
AvrII	CCTAGG	1 cut(s)	3704
AxyI	CCTNAGG	1 cut(s)	4293
BaeGI	GKGCMC	3 cut(s)	247, 2869, 3595
BaeI	ACNNNNGTAYC	4 cut(s)	105, 138, 5139, 5172
BalI	TGGCCA	1 cut(s)	5138
BanI	GGYRCC	3 cut(s)	433, 1612, 5462
BauI	CACGAG	3 cut(s)	2868, 3888, 4907
BbsI	GAAGAC	6 cut(s)	1281, 2027, 2936, 3271, 3528, 4737
Bbv12I	GWGCWC	4 cut(s)	2367, 2869, 3595, 4707
BccI	CCATC	9 cut(s)	325, 839, 1910, 2452, 3080, 3428, 3581, 4246, 4787
BceAI	ACGGC	3 cut(s)	74, 1609, 2167
BciVI	GTATCC	2 cut(s)	1402, 5073
BclI	TGATCA	4 cut(s)	1554, 1932, 2146, 5317
BcnI	CCSGG	2 cut(s)	527, 4652
BcoDI	GTCTC	5 cut(s)	392, 1953, 2677, 2946, 2954
BcuI	ACTAGT	2 cut(s)	3019, 4258
BfmI	CTRYAG	8 cut(s)	2221, 4168, 4185, 4389, 4606, 4632, 5000, 5193
BfrI	CTTAAG	2 cut(s)	2161, 3056
BfuAI	ACCTGC	1 cut(s)	4192
BfuI	GTATCC	2 cut(s)	1402, 5073
BglII	AGATCT	3 cut(s)	3368, 4787, 5438
BlnI	CCTAGG	1 cut(s)	3704
BlpI	GCTNAGC	1 cut(s)	1803
BmcAI	AGTACT	2 cut(s)	3719, 5346
Bme18I	GGWCC	4 cut(s)	768, 2156, 2461, 2771
BmeRI	GACNNNNNGTC	1 cut(s)	717
BmgBI	CACGTC	1 cut(s)	3176
BmgT120I	GGNCC	6 cut(s)	768, 2156, 2461, 2771, 3599, 3839
BmiI	GGNNCC	7 cut(s)	435, 925, 1614, 2158, 3757, 5208, 5464
BmrI	ACTGGG	3 cut(s)	4432, 5219, 5497
BmtI	GCTAGC	1 cut(s)	3923
BmuI	ACTGGG	3 cut(s)	4432, 5219, 5497
BpiI	GAAGAC	6 cut(s)	1281, 2027, 2936, 3271, 3528, 4737
BplI	GAGNNNNNCTC	2 cut(s)	2657, 2689
BpmI	CTGGAG	3 cut(s)	2528, 2928, 2985
Bpu10I	CCTNAGC	2 cut(s)	3849, 4992
Bpu1102I	GCTNAGC	1 cut(s)	1803
Bpu14I	TTCGAA	3 cut(s)	1405, 3780, 4738
BpuEI	CTTGAG	7 cut(s)	1524, 2005, 2515, 3341, 3350, 4025, 5432
BpuMI	CCSGG	2 cut(s)	527, 4652
BsaAI	YACGTR	2 cut(s)	4312, 4427
BsaBI	GATNNNNATC	1 cut(s)	4797
BsaHI	GRCGYC	1 cut(s)	4662
BsaI	GGTCTC	1 cut(s)	2677
BsaJI	CCNNGG	8 cut(s)	408, 927, 2132, 3400, 3596, 3704, 3810, 4020
BsaWI	WCCGGW	4 cut(s)	765, 1163, 1294, 2756
BsaXI	ACNNNNNCTCC	2 cut(s)	3147, 3177
Bse118I	RCCGGY	3 cut(s)	116, 765, 2784
Bse21I	CCTNAGG	1 cut(s)	4293
Bse3DI	GCAATG	4 cut(s)	2281, 2293, 5139, 5466
Bse8I	GATNNNNATC	1 cut(s)	4797
BseAI	TCCGGA	1 cut(s)	1294
BseDI	CCNNGG	8 cut(s)	408, 927, 2132, 3400, 3596, 3704, 3810, 4020
BseGI	GGATG	8 cut(s)	558, 603, 2332, 2484, 4160, 4332, 4762, 5407
BseJI	GATNNNNATC	1 cut(s)	4797
BseMI	GCAATG	4 cut(s)	2281, 2293, 5139, 5466
BseRI	GAGGAG	5 cut(s)	646, 1941, 2678, 2845, 3027
BseSI	GKGCMC	3 cut(s)	247, 2869, 3595
BseX3I	CGGCCG	1 cut(s)	87
BseYI	CCCAGC	2 cut(s)	3338, 4851
BsgI	GTGCAG	2 cut(s)	1740, 3510
Bsh1236I	CGCG	2 cut(s)	4022, 4570
Bsh1285I	CGRYCG	2 cut(s)	90, 131
BshFI	GGCC	8 cut(s)	89, 110, 1077, 3601, 3840, 4291, 5097, 5138
BshNI	GGYRCC	3 cut(s)	433, 1612, 5462
BshTI	ACCGGT	1 cut(s)	765
BsiEI	CGRYCG	2 cut(s)	90, 131
BsiHKAI	GWGCWC	4 cut(s)	2367, 2869, 3595, 4707
BslFI	GGGAC	7 cut(s)	53, 919, 1073, 2142, 3633, 4596, 5207
BsmAI	GTCTC	5 cut(s)	392, 1953, 2677, 2946, 2954
BsmBI	CGTCTC	1 cut(s)	2946
BsmFI	GGGAC	7 cut(s)	53, 919, 1073, 2142, 3633, 4596, 5207
BsmI	GAATGC	2 cut(s)	1853, 3340
BsnI	GGCC	8 cut(s)	89, 110, 1077, 3601, 3840, 4291, 5097, 5138
Bso31I	GGTCTC	1 cut(s)	2677
Bsp119I	TTCGAA	3 cut(s)	1405, 3780, 4738
Bsp1286I	GDGCHC	5 cut(s)	247, 2367, 2869, 3595, 4707
Bsp13I	TCCGGA	1 cut(s)	1294
Bsp1407I	TGTACA	1 cut(s)	2081
Bsp1720I	GCTNAGC	1 cut(s)	1803
Bsp19I	CCATGG	1 cut(s)	2132
BspACI	CCGC	8 cut(s)	77, 349, 1807, 2154, 2350, 3505, 4020, 4022
BspANI	GGCC	8 cut(s)	89, 110, 1077, 3601, 3840, 4291, 5097, 5138
BspEI	TCCGGA	1 cut(s)	1294
BspFNI	CGCG	2 cut(s)	4022, 4570
BspHI	TCATGA	3 cut(s)	183, 1528, 1924
BspLI	GGNNCC	7 cut(s)	435, 925, 1614, 2158, 3757, 5208, 5464
BspMAI	CTGCAG	5 cut(s)	2225, 4189, 4636, 5004, 5197
BspMI	ACCTGC	1 cut(s)	4192
BspOI	GCTAGC	1 cut(s)	3923
BspPI	GGATC	8 cut(s)	523, 583, 3839, 3952, 4044, 4539, 4680, 5493
BspT104I	TTCGAA	3 cut(s)	1405, 3780, 4738
BspT107I	GGYRCC	3 cut(s)	433, 1612, 5462
BspTI	CTTAAG	2 cut(s)	2161, 3056
BspTNI	GGTCTC	1 cut(s)	2677
BsrDI	GCAATG	4 cut(s)	2281, 2293, 5139, 5466
BsrFI	RCCGGY	3 cut(s)	116, 765, 2784
BsrGI	TGTACA	1 cut(s)	2081
BssAI	RCCGGY	3 cut(s)	116, 765, 2784
BssECI	CCNNGG	8 cut(s)	408, 927, 2132, 3400, 3596, 3704, 3810, 4020
BssNAI	GTATAC	3 cut(s)	5309, 5375, 5484
BssNI	GRCGYC	1 cut(s)	4662
BssSI	CACGAG	3 cut(s)	2868, 3888, 4907
BssT1I	CCWWGG	3 cut(s)	2132, 3400, 3704
Bst1107I	GTATAC	3 cut(s)	5309, 5375, 5484
Bst2BI	CACGAG	3 cut(s)	2868, 3888, 4907
Bst6I	CTCTTC	6 cut(s)	931, 1341, 1705, 2240, 4309, 4511
BstACI	GRCGYC	1 cut(s)	4662
BstAFI	CTTAAG	2 cut(s)	2161, 3056
BstAPI	GCANNNNNTGC	3 cut(s)	1799, 3521, 3899
BstAUI	TGTACA	1 cut(s)	2081
BstBAI	YACGTR	2 cut(s)	4312, 4427
BstBI	TTCGAA	3 cut(s)	1405, 3780, 4738
BstC8I	GCNNGC	9 cut(s)	2377, 2820, 3092, 3575, 3921, 4154, 4636, 5095, 5407
BstDSI	CCRYGG	3 cut(s)	927, 2132, 4020
BstF5I	GGATG	8 cut(s)	558, 603, 2332, 2484, 4160, 4332, 4762, 5407
BstFNI	CGCG	2 cut(s)	4022, 4570
BstHHI	GCGC	1 cut(s)	4572
BstMAI	GTCTC	5 cut(s)	392, 1953, 2677, 2946, 2954
BstMCI	CGRYCG	2 cut(s)	90, 131
BstMWI	GCNNNNNNNGC	8 cut(s)	86, 1799, 2993, 3521, 3739, 3899, 3929, 5099
BstNSI	RCATGY	6 cut(s)	343, 1604, 2088, 2379, 3491, 3577
BstSFI	CTRYAG	8 cut(s)	2221, 4168, 4185, 4389, 4606, 4632, 5000, 5193
BstSLI	GKGCMC	3 cut(s)	247, 2869, 3595
BstSNI	TACGTA	1 cut(s)	4312
BstUI	CGCG	2 cut(s)	4022, 4570
BstV2I	GAAGAC	6 cut(s)	1281, 2027, 2936, 3271, 3528, 4737
BstX2I	RGATCY	8 cut(s)	515, 3368, 4036, 4531, 4685, 4787, 5438, 5498
BstYI	RGATCY	8 cut(s)	515, 3368, 4036, 4531, 4685, 4787, 5438, 5498
BstZ17I	GTATAC	3 cut(s)	5309, 5375, 5484
BstZI	CGGCCG	1 cut(s)	87
Bsu36I	CCTNAGG	1 cut(s)	4293
BsuI	GTATCC	2 cut(s)	1402, 5073
BsuRI	GGCC	8 cut(s)	89, 110, 1077, 3601, 3840, 4291, 5097, 5138
BtgI	CCRYGG	3 cut(s)	927, 2132, 4020
BtrI	CACGTC	1 cut(s)	3176
BtsCI	GGATG	8 cut(s)	558, 603, 2332, 2484, 4160, 4332, 4762, 5407
BveI	ACCTGC	1 cut(s)	4192
Cac8I	GCNNGC	9 cut(s)	2377, 2820, 3092, 3575, 3921, 4154, 4636, 5095, 5407
CaiI	CAGNNNCTG	5 cut(s)	2963, 3290, 3454, 4444, 4999
CciI	TCATGA	3 cut(s)	183, 1528, 1924
CfoI	GCGC	1 cut(s)	4572
Cfr10I	RCCGGY	3 cut(s)	116, 765, 2784
Cfr13I	GGNCC	6 cut(s)	768, 2156, 2461, 2771, 3599, 3839
Cfr42I	CCGCGG	1 cut(s)	4023
CsiI	ACCWGGT	1 cut(s)	1386
CspAI	ACCGGT	1 cut(s)	765
DraI	TTTAAA	1 cut(s)	5122
DrdI	GACNNNNNNGTC	2 cut(s)	2938, 3932
DriI	GACNNNNNGTC	1 cut(s)	717
DseDI	GACNNNNNNGTC	2 cut(s)	2938, 3932
EaeI	YGGCCR	2 cut(s)	87, 5136
EagI	CGGCCG	1 cut(s)	87
Eam1104I	CTCTTC	6 cut(s)	931, 1341, 1705, 2240, 4309, 4511
Eam1105I	GACNNNNNGTC	1 cut(s)	717
EarI	CTCTTC	6 cut(s)	931, 1341, 1705, 2240, 4309, 4511
EclXI	CGGCCG	1 cut(s)	87
Eco105I	TACGTA	1 cut(s)	4312
Eco130I	CCWWGG	3 cut(s)	2132, 3400, 3704
Eco147I	AGGCCT	2 cut(s)	1077, 4291
Eco31I	GGTCTC	1 cut(s)	2677
Eco32I	GATATC	1 cut(s)	4595
Eco47I	GGWCC	4 cut(s)	768, 2156, 2461, 2771
Eco52I	CGGCCG	1 cut(s)	87
Eco81I	CCTNAGG	1 cut(s)	4293
EcoO109I	RGGNCCY	2 cut(s)	2771, 3839
EcoRI	GAATTC	1 cut(s)	4555
EcoRV	GATATC	1 cut(s)	4595
EcoT14I	CCWWGG	3 cut(s)	2132, 3400, 3704
EcoT22I	ATGCAT	3 cut(s)	1853, 2332, 4345
ErhI	CCWWGG	3 cut(s)	2132, 3400, 3704
Esp3I	CGTCTC	1 cut(s)	2946
FalI	AAGNNNNNCTT	6 cut(s)	928, 960, 1079, 1111, 4581, 4613
FaqI	GGGAC	7 cut(s)	53, 919, 1073, 2142, 3633, 4596, 5207
FauNDI	CATATG	2 cut(s)	537, 2989
FbaI	TGATCA	4 cut(s)	1554, 1932, 2146, 5317
FblI	GTMKAC	5 cut(s)	5, 4423, 5308, 5374, 5483
FokI	GGATG	8 cut(s)	545, 590, 2339, 2471, 4147, 4319, 4769, 5414
GlaI	GCGC	1 cut(s)	4571
GsaI	CCCAGC	2 cut(s)	3342, 4855
GsuI	CTGGAG	3 cut(s)	2528, 2928, 2985
HaeIII	GGCC	8 cut(s)	89, 110, 1077, 3601, 3840, 4291, 5097, 5138
HhaI	GCGC	1 cut(s)	4572
Hin1I	GRCGYC	1 cut(s)	4662
Hin6I	GCGC	1 cut(s)	4570
HinP1I	GCGC	1 cut(s)	4570
HincII	GTYRAC	2 cut(s)	2215, 2780
HindII	GTYRAC	2 cut(s)	2215, 2780
HindIII	AAGCTT	1 cut(s)	4982
HphI	GGTGA	8 cut(s)	154, 245, 570, 1352, 3797, 4406, 4426, 4928
Hpy99I	CGWCG	3 cut(s)	136, 3177, 4667
HpyCH4IV	ACGT	8 cut(s)	3175, 4147, 4311, 4329, 4426, 4662, 4926, 5154
HpyF10VI	GCNNNNNNNGC	8 cut(s)	86, 1799, 2993, 3521, 3739, 3899, 3929, 5099
HpySE526I	ACGT	8 cut(s)	3175, 4147, 4311, 4329, 4426, 4662, 4926, 5154
Hsp92I	GRCGYC	1 cut(s)	4662
HspAI	GCGC	1 cut(s)	4570
Kpn2I	TCCGGA	1 cut(s)	1294
Ksp22I	TGATCA	4 cut(s)	1554, 1932, 2146, 5317
KspI	CCGCGG	1 cut(s)	4023
MabI	ACCWGGT	1 cut(s)	1386
MaeII	ACGT	8 cut(s)	3175, 4147, 4311, 4329, 4426, 4662, 4926, 5154
MfeI	CAATTG	2 cut(s)	740, 2393
MflI	RGATCY	8 cut(s)	515, 3368, 4036, 4531, 4685, 4787, 5438, 5498
MhlI	GDGCHC	5 cut(s)	247, 2367, 2869, 3595, 4707
MlsI	TGGCCA	1 cut(s)	5138
MluNI	TGGCCA	1 cut(s)	5138
MlyI	GAGTC	5 cut(s)	200, 1295, 2054, 2257, 2506
MmeI	TCCRAC	5 cut(s)	183, 1807, 3081, 3133, 5200
Mox20I	TGGCCA	1 cut(s)	5138
Mph1103I	ATGCAT	3 cut(s)	1853, 2332, 4345
MroI	TCCGGA	1 cut(s)	1294
MroXI	GAANNNNTTC	5 cut(s)	875, 2480, 4380, 4734, 5243
MscI	TGGCCA	1 cut(s)	5138
Msp20I	TGGCCA	1 cut(s)	5138
MspA1I	CMGCKG	2 cut(s)	4022, 4855
MspCI	CTTAAG	2 cut(s)	2161, 3056
MunI	CAATTG	2 cut(s)	740, 2393
Mva1269I	GAATGC	2 cut(s)	1853, 3340
MvnI	CGCG	2 cut(s)	4022, 4570
MwoI	GCNNNNNNNGC	8 cut(s)	86, 1799, 2993, 3521, 3739, 3899, 3929, 5099
NciI	CCSGG	2 cut(s)	527, 4652
NcoI	CCATGG	1 cut(s)	2132
NdeI	CATATG	2 cut(s)	537, 2989
NheI	GCTAGC	1 cut(s)	3919
NlaIV	GGNNCC	7 cut(s)	435, 925, 1614, 2158, 3757, 5208, 5464
NmuCI	GTSAC	5 cut(s)	251, 391, 1784, 4624, 5188
NsiI	ATGCAT	3 cut(s)	1853, 2332, 4345
NspI	RCATGY	6 cut(s)	343, 1604, 2088, 2379, 3491, 3577
NspV	TTCGAA	3 cut(s)	1405, 3780, 4738
OliI	CACNNNNGTG	1 cut(s)	2430
PaeI	GCATGC	2 cut(s)	2379, 3577
PagI	TCATGA	3 cut(s)	183, 1528, 1924
PceI	AGGCCT	2 cut(s)	1077, 4291
PciI	ACATGT	2 cut(s)	339, 2084
PcsI	WCGNNNNNNNCGW	1 cut(s)	3172
PctI	GAATGC	2 cut(s)	1853, 3340
PdmI	GAANNNNTTC	5 cut(s)	875, 2480, 4380, 4734, 5243
PflFI	GACNNNGTC	1 cut(s)	2775
PflMI	CCANNNNNTGG	4 cut(s)	25, 202, 1056, 4444
PfoI	TCCNGGA	1 cut(s)	1477
PinAI	ACCGGT	1 cut(s)	765
PleI	GAGTC	5 cut(s)	200, 1294, 2054, 2256, 2505
PpsI	GAGTC	5 cut(s)	200, 1294, 2054, 2256, 2505
Ppu21I	YACGTR	2 cut(s)	4312, 4427
PpuMI	RGGWCCY	1 cut(s)	2771
PscI	ACATGT	2 cut(s)	339, 2084
PshBI	ATTAAT	4 cut(s)	384, 2904, 3870, 4218
PsiI	TTATAA	2 cut(s)	3713, 3915
Psp1406I	AACGTT	1 cut(s)	4926
Psp5II	RGGWCCY	1 cut(s)	2771
PspFI	CCCAGC	2 cut(s)	3338, 4851
PspN4I	GGNNCC	7 cut(s)	435, 925, 1614, 2158, 3757, 5208, 5464
PspPI	GGNCC	6 cut(s)	768, 2156, 2461, 2771, 3599, 3839
PspPPI	RGGWCCY	1 cut(s)	2771
PstI	CTGCAG	5 cut(s)	2225, 4189, 4636, 5004, 5197
PstNI	CAGNNNCTG	5 cut(s)	2963, 3290, 3454, 4444, 4999
PsuI	RGATCY	8 cut(s)	515, 3368, 4036, 4531, 4685, 4787, 5438, 5498
PsyI	GACNNNGTC	1 cut(s)	2775
PvuII	CAGCTG	1 cut(s)	4855
SacII	CCGCGG	1 cut(s)	4023
Sau96I	GGNCC	6 cut(s)	768, 2156, 2461, 2771, 3599, 3839
SbfI	CCTGCAGG	1 cut(s)	4636
ScaI	AGTACT	2 cut(s)	3719, 5346
SchI	GAGTC	5 cut(s)	200, 1295, 2054, 2257, 2506
SdaI	CCTGCAGG	1 cut(s)	4636
SduI	GDGCHC	5 cut(s)	247, 2367, 2869, 3595, 4707
SexAI	ACCWGGT	1 cut(s)	1386
SfcI	CTRYAG	8 cut(s)	2221, 4168, 4185, 4389, 4606, 4632, 5000, 5193
Sfr303I	CCGCGG	1 cut(s)	4023
SfuI	TTCGAA	3 cut(s)	1405, 3780, 4738
SgrBI	CCGCGG	1 cut(s)	4023
SinI	GGWCC	4 cut(s)	768, 2156, 2461, 2771
SmlI	CTYRAG	9 cut(s)	1539, 1984, 2161, 2494, 3056, 3320, 3329, 4004, 5411
SmoI	CTYRAG	9 cut(s)	1539, 1984, 2161, 2494, 3056, 3320, 3329, 4004, 5411
SnaBI	TACGTA	1 cut(s)	4312
SpeI	ACTAGT	2 cut(s)	3019, 4258
SphI	GCATGC	2 cut(s)	2379, 3577
Sse8387I	CCTGCAGG	1 cut(s)	4636
SseBI	AGGCCT	2 cut(s)	1077, 4291
SsiI	CCGC	8 cut(s)	77, 349, 1807, 2154, 2350, 3505, 4020, 4022
SspI	AATATT	1 cut(s)	4199
StuI	AGGCCT	2 cut(s)	1077, 4291
StyI	CCWWGG	3 cut(s)	2132, 3400, 3704
TaiI	ACGT	8 cut(s)	3178, 4150, 4314, 4332, 4429, 4665, 4929, 5157
TaqI	TCGA	9 cut(s)	153, 1405, 1470, 2038, 3172, 3780, 4665, 4738, 5217
TaqII	GACCGA	2 cut(s)	117, 4612
TatI	WGTACW	5 cut(s)	548, 2081, 2104, 3717, 5344
TauI	GCSGC	3 cut(s)	80, 351, 1810
TseFI	GTSAC	5 cut(s)	251, 391, 1784, 4624, 5188
Tsp45I	GTSAC	5 cut(s)	251, 391, 1784, 4624, 5188
TspGWI	ACGGA	4 cut(s)	2654, 3529, 3748, 5119
Tth111I	GACNNNGTC	1 cut(s)	2775
Van91I	CCANNNNNTGG	4 cut(s)	25, 202, 1056, 4444
Vha464I	CTTAAG	2 cut(s)	2161, 3056
VneI	GTGCAC	2 cut(s)	2865, 3591
VpaK11BI	GGWCC	4 cut(s)	768, 2156, 2461, 2771
VspI	ATTAAT	4 cut(s)	384, 2904, 3870, 4218
XbaI	TCTAGA	3 cut(s)	3259, 3628, 5006
XceI	RCATGY	6 cut(s)	343, 1604, 2088, 2379, 3491, 3577
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3407, 4424
XmaJI	CCTAGG	1 cut(s)	3704
XmiI	GTMKAC	5 cut(s)	5, 4423, 5308, 5374, 5483
XmnI	GAANNNNTTC	5 cut(s)	875, 2480, 4380, 4734, 5243
ZraI	GACGTC	1 cut(s)	4663
ZrmI	AGTACT	2 cut(s)	3719, 5346
Zsp2I	ATGCAT	3 cut(s)	1853, 2332, 4345

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.