Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_ssc0000482.1

Orthogroup ID

OG0008435

Physical Location & Seq

Genomic Coordinates Reverse (-)

94321 .. 115371

Gene Structure 21051 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_ssc0000482.1_g000025.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5355 bp

atggctactcttatcacttcactctccaccagcttcagccaagtccctccggcggctgttccggccgtcttggattgcattttggcctctaccggcgtatctcggtcgtcgctattcgcttcgcttctcgacactttcaccaagctctctgagggggtcatgaaaaatgaccagaagttggactcccatgactgtaactatctggcatcattggttggggcactttgtcaccttctaaagaaatttgacactaatcttggtgctttggaatcatttatatggaaatgttttcttccattgataaagatggcacacatgttcagccgcaatatgcttaaccagattacagaatcgtttattaatgctgtgactgagacaaacacctgggtagttgtggaggcaacactggtgccaatttttctaacattagttgccagttctattggtatgcctgaaaaggaagtatcagatacttttcagttcatgttgggatctaatgaccgggtaaatcatatggaaatgagtacaaaaaacatcctttcgccacctggatcaccttcgctgccaatttcatgccacatcctaaccttaatgcttgatgctgtccttcaaagcaagaaaatagctgcctcctcagaatcaataattgcaaatgggtgttttgatgataattttgctggtaaattgacttggggtctttgtagcttgactgagcaattgcttttacaaaatttggaacaccggtcctgtgctactgcctttcttctcccaatcattttcaaagcattttcttcttattgctcttttcaaatctcaatagatgggcagacctttacactttcaaggaacaacttctttatgaaaatttggaagtgctgtgggacactgttttctcttggaaccgtggagagaagagatgcttttggtgtactctccttgtacctgtctttttttccttttacagaagaaagtggagataccaacatgagtgataaagtggaatgttttgatataagagctgaaaccaaattttgggacgagattaaaagaggcctggttgataaggagagcttagtgaggaagcaatcgttacacatcttaaggatagcactacgcataaatggagcagcccaatcctccggtgttttagaggatatatcggctgaaaataattcaatgcctcacagcatgacaaagagggagctgtgggctgataaggaagctaaatctctgggtgtggggaaaatatgtagccaagttgagtcttctccggacagccagaacaagtgggaggcatttgtattgctgtatgaaatgcttgaagagtatggaactcacctcgttgaagcagcttggaattaccaggtatccttgttacttcgaaactctagctcagatgctaataataaaacctctatcagtggagcagttcatcaaaaccagatcgaaagtcctggagaaatctttaactggttagcaattttgtgggaacggggttttaatcatgacaatcctcaagttaggtgcttgatcatgcagtcatttttagggattgattggaagaattacggcacacatgcaagatcggtgcctggatattttgttcttggttcatttatacaagggctaaatgaccctgtgcatcacaaagaatttggtgtaaaaggaatatactcttcaatgacaatagaaggtgcagctaactttctaagcgaatatacaagttatctgaatgtgaggaagtgcattatgtttttgagtgacttagcatctgctgctaagcggcaacctcttggtagagttggattgatgtgccttgctgaatgcatttcttctgctgcttgtctagtcagaaagaataatagtgaaattgatgcacagtgctttgaagatggagttcatgacatgatcaaagtgggaaagtctcctcacaatgacaaaattgttttgcttgatgccttgagatttattattgagagcagcaaacaacatttcaattctaattatcgtcttcgagtttgtgagaaagtccttgactctgctgcttcagtggtgtgtacatgtgatctgcctcttgatgtacttttgcacttcatttcaacatttccatgggagttcactgatcacggcggtcccttaagagagaaaatacgacaatggcttatggggtgtggtaagcgttgtagtgtcaactgctgcagttctgagatgaagctcctgaagagtctttatgactttccaaataggtttacgagccattgcaatgatgatgcttcaaccacttttgacgatgaagatttggatgcatgggaatttgaagcgaagcggtggacaagagtgctctttattgcatgcaaggaggaatatcaattgttacccatactgctgtttattcaaaatcacggtagtgatgtctgcaaacaaaacaataagatggacctatttcctgtgaagtttttcatcctcatcttgagtctagttctggagcttcagatgatgcaagaaagagttgccaaacatggttctaagcttagaaccaaatcagagttttttgtgccagaatcaatgaatgaatttagtcatgcagatgttcctaatttatatcaaaagttcacggacatttttcttctcatattggaggagttggtctcttttgcaaacttgtcttgttccatattctcaaataacaataccactacgatgagagatgcaacccttcctggttctgtaaccggaaaactcggaggacctagtcaacgccggttgtcatcttctaccactactgctgtattgcaagcaactggagcagaaatacgtcttgcagcatatgaagcattagctcctactctcacagcgctagtgtctgtgtgttctcctcaaactttggatatacttaagaaaaatgacagctcattactatcagatggcaaacctctgttggattctttggtgctttcttttcttcagaacataaataagctccttacagttggagtatttgtacgatctcgacgtgcaattttaatgaattggaagtggatttgcctggaatctctactgtcaattccttgctatgctcttaggaatgggcttcttctagaggcaaataattttgtcttctcaggtgctgctctccgatgtattttttctgatattcttgagagccttgagaatgctggggaaggttctgttttaccgatgctcagatctgtccgattagttttggacttatttgcccaaggaaagtctggttcacttgtctgcttatgtgatggggtggattcccagatgctgtggcatttggttcattcttcttggatattgcatgtcagttgcaacaagcggaaggttgcacctattgctgcacttttgtcttccgttctccattcatctctatttagtgatgagagcatgcacataattgatggtgcacctgggcctctgaagtggtttgttgagaaaattctagaggaaggcacgaaaagtccccgtacaattcgtcttgctgcattacacttaacagggctgtggctatcatatcctaggattataaagtactatgtgaaggagctgaagctgctatctctacatggttccgttgcatttgatgaggacttcgaaggtgaattagctgataatcgtgataccagggctgaagtttctttgttggccaaaggccctgatcctgagctgaccaaagcatttattaatacagagttgtatgcacgagcctctgttgctgttttgttttataagctagcagacatggccgtcatggtgggatcaactaatgaaaatgcagattgccatgcagctttggaatctggaaaaatgtttttgcttgagcttcttgattccgcggtaaacgaaaaggatcttgcaaaagagttgtataagaagtatagtgcaattcatagaaggaaggtacgggcatggcaaatgatatgtattttgtcgcatttcgtctgtcaagatatagtttcagaagttgcacgttgcttgcacacatccctctataggaacaacttacctgcagttcgtcaatatttggaaacatttgcaattaatatatacttaaagtttccatcactggttggggagcaactagttcctatattaagagattatgacatgaggcctcaggctctctcttcttacgtatttatagcagctaacgtcatcctccatgcatctcaatctgttcaacaccgacatctgaatgaattacttcctcctatagttccactattaacctcacatcaccacagtctacgtggtttcacccagttgctggtttaccaagttctttgcaaattgttccctccattggattctaaagcctctgaaactatgactttagagaagaggtgttttgaggatctcaaatcgtacctcgcaaagaattctgattgtacgcgcttacgacaatcaatggaaggatatcttgatgcctacagtccccatagctcggtcactcctgcaggcgttttcatcaaccgggttgaggacgtcgagtttgaatgtgtaccaagatcccttatggagcaagtgctcaactttttgaatgatgtgagggaagaccttcgaaattcaatggcaaaggatgttatgactattaagaatgagagtttgagatctgatggagatcaagaccacatggcgatactgtctaatgctaatgaaagaaaattgagtacccagctgcctaaagatacctcactagattttcagaagaagattactctgtctaaacacgagaagcaagacataaacgttaattctcaccaagaaacttacaaacaacttcaggaagtagaaaaggacgataagcttcttgctcaggtgctgcagtctagaactctaggaatggaaagagagagatcaagcagacaacatcttatccttgtagcatctctgcttgatcggatacctaacctagctggccttgcacggacttgtgaggtatttaaagcatcaagtttggccattgcagatacgaacgtgttacatgacaaacagttccaactcatcagtgtgactgcagagaagtgggttcctatcatcgaagtcccagtgaatagtttgaaggtgttccttcagaaaaagaaacgagaaggcttctcaattttaggactagagcagactgcaaatagtatacctcttgatcaatatatatttccaaaagacacagtactggttcttgggcgagagaaggaaggtataccagttgatataatacatattctggatgcttgccttgagattccacagttgggagttgttagatctctcaatgttcatgtcagtggtgccattgctctgtgggagtatactagacagcagagatcccagtag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000451 GO:0000453 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006355 GO:0006357 GO:0006364 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008173 GO:0008175 GO:0008649 GO:0008757 GO:0009451 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016072 GO:0016423 GO:0016435 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0022613 GO:0030488 GO:0031167 GO:0031323 GO:0031326 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070039 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:0140102 GO:1901360 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SpoU_methylase

Protein Analysis

1784

Amino Acids

200.1

Weight (kDa)

6.24

Isoelectric Point (pI)

45.75

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008435)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17610 AT4G17610 AT4G17610
fragaria_vesca	FvH4_5g20370 FvH4_5g20370 FvH4_5g20370 FvH4_5g20370 FvH4_5g20370
malus_domestica	MD04G1055600.v1.1
prunus_persica	Prupe.5G055400_v2.0.a1 Prupe.5G055400_v2.0.a1
pyrus_communis	pycom04g04890
rosa_chinensis	RchiOBHm_Chr7g0205631
rosa_laevigata	RLG00000003369
rosa_multiflora	Rmu_ssc0000482.1_g000025
rosa_roxburghii	Rroxscaffold_3G00252130
rosa_rugosa	Rorug07G0092300
rosa_samantha	Rh7AG223100 Rh7AG230100 Rh7BG218100 Rh7CG236300 Rh7DG230000
rosa_wichuraiana	Rw7G019240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	3551, 3753
AasI	GACNNNNNNGTC	1 cut(s)	3770
AatII	GACGTC	1 cut(s)	4512
Acc36I	ACCTGC	1 cut(s)	4039
AccB1I	GGYRCC	3 cut(s)	409, 1588, 5309
AccB7I	CCANNNNNTGG	3 cut(s)	178, 1032, 4291
AccI	GTMKAC	4 cut(s)	4270, 5155, 5221, 5330
AccII	CGCG	2 cut(s)	3860, 4417
AccIII	TCCGGA	1 cut(s)	1270
AciI	CCGC	8 cut(s)	53, 325, 1783, 2130, 2326, 3343, 3858, 3860
AclI	AACGTT	1 cut(s)	4773
AclWI	GGATC	8 cut(s)	499, 559, 3677, 3790, 3882, 4386, 4527, 5340
AcoI	YGGCCR	4 cut(s)	63, 3669, 3768, 4983
AcyI	GRCGYC	1 cut(s)	4509
AfeI	AGCGCT	1 cut(s)	2856
AfiI	CCNNNNNNNGG	9 cut(s)	52, 178, 1032, 4017, 4291, 4327, 4468, 4504, 5273
AflII	CTTAAG	3 cut(s)	1099, 2137, 2894
AflIII	ACRYGT	3 cut(s)	315, 2060, 5001
AgeI	ACCGGT	1 cut(s)	741
AhdI	GACNNNNNGTC	1 cut(s)	693
AhlI	ACTAGT	1 cut(s)	4105
AjiI	CACGTC	1 cut(s)	3014
AleI	CACNNNNGTG	1 cut(s)	2406
AloI	GAACNNNNNNTCC	2 cut(s)	1587, 1619
Alw21I	GWGCWC	3 cut(s)	2343, 3433, 4554
Alw26I	GTCTC	3 cut(s)	368, 1929, 2653
Alw44I	GTGCAC	1 cut(s)	3429
AlwI	GGATC	8 cut(s)	499, 559, 3677, 3790, 3882, 4386, 4527, 5340
AlwNI	CAGNNNCTG	4 cut(s)	3128, 3292, 4291, 4846
Aor13HI	TCCGGA	1 cut(s)	1270
Aor51HI	AGCGCT	1 cut(s)	2856
ApaLI	GTGCAC	1 cut(s)	3429
ArsI	GACNNNNNNTTYG	2 cut(s)	4499, 4531
AseI	ATTAAT	3 cut(s)	360, 3708, 4065
AsiGI	ACCGGT	1 cut(s)	741
Asp700I	GAANNNNTTC	5 cut(s)	851, 2456, 4227, 4581, 5090
AspA2I	CCTAGG	1 cut(s)	3542
AspLEI	GCGC	2 cut(s)	2857, 4419
AspS9I	GGNCC	6 cut(s)	744, 2132, 2437, 2747, 3437, 3677
AsuC2I	CCSGG	2 cut(s)	503, 4499
AsuHPI	GGTGA	8 cut(s)	130, 221, 546, 1328, 3635, 4253, 4273, 4775
AsuII	TTCGAA	3 cut(s)	1381, 3618, 4585
AsuNHI	GCTAGC	1 cut(s)	3757
AvaII	GGWCC	4 cut(s)	744, 2132, 2437, 2747
AvrII	CCTAGG	1 cut(s)	3542
AxyI	CCTNAGG	1 cut(s)	4140
BaeGI	GKGCMC	2 cut(s)	223, 3433
BaeI	ACNNNNGTAYC	4 cut(s)	81, 114, 4986, 5019
BalI	TGGCCA	2 cut(s)	3671, 4985
BanI	GGYRCC	3 cut(s)	409, 1588, 5309
BauI	CACGAG	2 cut(s)	3726, 4754
BbsI	GAAGAC	5 cut(s)	1257, 2003, 3109, 3366, 4584
Bbv12I	GWGCWC	3 cut(s)	2343, 3433, 4554
BccI	CCATC	9 cut(s)	301, 815, 1886, 2428, 2918, 3266, 3419, 4093, 4634
BceAI	ACGGC	4 cut(s)	50, 1585, 2143, 3755
BciVI	GTATCC	2 cut(s)	1378, 4920
BclI	TGATCA	4 cut(s)	1530, 1908, 2122, 5164
BcnI	CCSGG	2 cut(s)	503, 4499
BcoDI	GTCTC	3 cut(s)	368, 1929, 2653
BcuI	ACTAGT	1 cut(s)	4105
BfmI	CTRYAG	8 cut(s)	2197, 4015, 4032, 4236, 4453, 4479, 4847, 5040
BfoI	RGCGCY	1 cut(s)	2858
BfrI	CTTAAG	3 cut(s)	1099, 2137, 2894
BfuAI	ACCTGC	1 cut(s)	4039
BfuI	GTATCC	2 cut(s)	1378, 4920
BglII	AGATCT	3 cut(s)	3206, 4634, 5285
BlnI	CCTAGG	1 cut(s)	3542
BlpI	GCTNAGC	1 cut(s)	1779
BmcAI	AGTACT	2 cut(s)	3557, 5193
Bme18I	GGWCC	4 cut(s)	744, 2132, 2437, 2747
BmeRI	GACNNNNNGTC	1 cut(s)	693
BmgBI	CACGTC	1 cut(s)	3014
BmgT120I	GGNCC	6 cut(s)	744, 2132, 2437, 2747, 3437, 3677
BmiI	GGNNCC	7 cut(s)	411, 901, 1590, 2134, 3595, 5055, 5311
BmrI	ACTGGG	3 cut(s)	4279, 5066, 5344
BmtI	GCTAGC	1 cut(s)	3761
BmuI	ACTGGG	3 cut(s)	4279, 5066, 5344
BpiI	GAAGAC	5 cut(s)	1257, 2003, 3109, 3366, 4584
BplI	GAGNNNNNCTC	2 cut(s)	2633, 2665
BpmI	CTGGAG	3 cut(s)	1476, 2504, 2823
Bpu10I	CCTNAGC	2 cut(s)	3687, 4839
Bpu1102I	GCTNAGC	1 cut(s)	1779
Bpu14I	TTCGAA	3 cut(s)	1381, 3618, 4585
BpuEI	CTTGAG	7 cut(s)	1500, 1981, 2491, 3179, 3188, 3863, 5279
BpuMI	CCSGG	2 cut(s)	503, 4499
BsaAI	YACGTR	2 cut(s)	4159, 4274
BsaBI	GATNNNNATC	1 cut(s)	4644
BsaHI	GRCGYC	1 cut(s)	4509
BsaI	GGTCTC	1 cut(s)	2653
BsaJI	CCNNGG	8 cut(s)	384, 903, 2108, 3238, 3434, 3542, 3648, 3858
BsaWI	WCCGGW	4 cut(s)	741, 1139, 1270, 2732
BsaXI	ACNNNNNCTCC	2 cut(s)	2985, 3015
Bsc4I	CCNNNNNNNGG	9 cut(s)	52, 178, 1032, 4017, 4291, 4327, 4468, 4504, 5273
Bse118I	RCCGGY	3 cut(s)	92, 741, 2760
Bse21I	CCTNAGG	1 cut(s)	4140
Bse3DI	GCAATG	4 cut(s)	2257, 2269, 4986, 5313
Bse8I	GATNNNNATC	1 cut(s)	4644
BseAI	TCCGGA	1 cut(s)	1270
BseDI	CCNNGG	8 cut(s)	384, 903, 2108, 3238, 3434, 3542, 3648, 3858
BseGI	GGATG	8 cut(s)	534, 579, 2308, 2460, 4007, 4179, 4609, 5254
BseJI	GATNNNNATC	1 cut(s)	4644
BseLI	CCNNNNNNNGG	9 cut(s)	52, 178, 1032, 4017, 4291, 4327, 4468, 4504, 5273
BseMI	GCAATG	4 cut(s)	2257, 2269, 4986, 5313
BseRI	GAGGAG	4 cut(s)	622, 1917, 2654, 2865
BseSI	GKGCMC	2 cut(s)	223, 3433
BseX3I	CGGCCG	1 cut(s)	63
BseYI	CCCAGC	2 cut(s)	3176, 4698
BsgI	GTGCAG	2 cut(s)	1716, 3348
Bsh1236I	CGCG	2 cut(s)	3860, 4417
Bsh1285I	CGRYCG	2 cut(s)	66, 107
BshNI	GGYRCC	3 cut(s)	409, 1588, 5309
BshTI	ACCGGT	1 cut(s)	741
BsiEI	CGRYCG	2 cut(s)	66, 107
BsiHKAI	GWGCWC	3 cut(s)	2343, 3433, 4554
BslFI	GGGAC	7 cut(s)	29, 895, 1049, 2118, 3471, 4443, 5054
BslI	CCNNNNNNNGG	9 cut(s)	52, 178, 1032, 4017, 4291, 4327, 4468, 4504, 5273
BsmAI	GTCTC	3 cut(s)	368, 1929, 2653
BsmFI	GGGAC	7 cut(s)	29, 895, 1049, 2118, 3471, 4443, 5054
BsmI	GAATGC	2 cut(s)	1829, 3178
Bso31I	GGTCTC	1 cut(s)	2653
Bsp119I	TTCGAA	3 cut(s)	1381, 3618, 4585
Bsp1286I	GDGCHC	4 cut(s)	223, 2343, 3433, 4554
Bsp13I	TCCGGA	1 cut(s)	1270
Bsp1407I	TGTACA	1 cut(s)	2057
Bsp1720I	GCTNAGC	1 cut(s)	1779
Bsp19I	CCATGG	1 cut(s)	2108
BspACI	CCGC	8 cut(s)	53, 325, 1783, 2130, 2326, 3343, 3858, 3860
BspEI	TCCGGA	1 cut(s)	1270
BspFNI	CGCG	2 cut(s)	3860, 4417
BspHI	TCATGA	3 cut(s)	159, 1504, 1900
BspLI	GGNNCC	7 cut(s)	411, 901, 1590, 2134, 3595, 5055, 5311
BspMAI	CTGCAG	5 cut(s)	2201, 4036, 4483, 4851, 5044
BspMI	ACCTGC	1 cut(s)	4039
BspOI	GCTAGC	1 cut(s)	3761
BspPI	GGATC	8 cut(s)	499, 559, 3677, 3790, 3882, 4386, 4527, 5340
BspT104I	TTCGAA	3 cut(s)	1381, 3618, 4585
BspT107I	GGYRCC	3 cut(s)	409, 1588, 5309
BspTI	CTTAAG	3 cut(s)	1099, 2137, 2894
BspTNI	GGTCTC	1 cut(s)	2653
BsrDI	GCAATG	4 cut(s)	2257, 2269, 4986, 5313
BsrFI	RCCGGY	3 cut(s)	92, 741, 2760
BsrGI	TGTACA	1 cut(s)	2057
BssAI	RCCGGY	3 cut(s)	92, 741, 2760
BssECI	CCNNGG	8 cut(s)	384, 903, 2108, 3238, 3434, 3542, 3648, 3858
BssNAI	GTATAC	3 cut(s)	5156, 5222, 5331
BssNI	GRCGYC	1 cut(s)	4509
BssSI	CACGAG	2 cut(s)	3726, 4754
BssT1I	CCWWGG	3 cut(s)	2108, 3238, 3542
Bst1107I	GTATAC	3 cut(s)	5156, 5222, 5331
Bst2BI	CACGAG	2 cut(s)	3726, 4754
Bst6I	CTCTTC	6 cut(s)	907, 1317, 1681, 2216, 4156, 4358
BstACI	GRCGYC	1 cut(s)	4509
BstAFI	CTTAAG	3 cut(s)	1099, 2137, 2894
BstAPI	GCANNNNNTGC	2 cut(s)	1775, 3359
BstAUI	TGTACA	1 cut(s)	2057
BstBAI	YACGTR	2 cut(s)	4159, 4274
BstBI	TTCGAA	3 cut(s)	1381, 3618, 4585
BstC8I	GCNNGC	8 cut(s)	2353, 2796, 3413, 3759, 4001, 4483, 4942, 5254
BstDSI	CCRYGG	3 cut(s)	903, 2108, 3858
BstF5I	GGATG	8 cut(s)	534, 579, 2308, 2460, 4007, 4179, 4609, 5254
BstFNI	CGCG	2 cut(s)	3860, 4417
BstH2I	RGCGCY	1 cut(s)	2858
BstHHI	GCGC	2 cut(s)	2857, 4419
BstMAI	GTCTC	3 cut(s)	368, 1929, 2653
BstMCI	CGRYCG	2 cut(s)	66, 107
BstMWI	GCNNNNNNNGC	9 cut(s)	62, 1775, 2804, 2831, 3359, 3577, 3737, 3767, 4946
BstNSI	RCATGY	6 cut(s)	319, 1580, 2064, 2355, 3329, 3415
BstSFI	CTRYAG	8 cut(s)	2197, 4015, 4032, 4236, 4453, 4479, 4847, 5040
BstSLI	GKGCMC	2 cut(s)	223, 3433
BstSNI	TACGTA	1 cut(s)	4159
BstUI	CGCG	2 cut(s)	3860, 4417
BstV2I	GAAGAC	5 cut(s)	1257, 2003, 3109, 3366, 4584
BstX2I	RGATCY	8 cut(s)	491, 3206, 3874, 4378, 4532, 4634, 5285, 5345
BstYI	RGATCY	8 cut(s)	491, 3206, 3874, 4378, 4532, 4634, 5285, 5345
BstZ17I	GTATAC	3 cut(s)	5156, 5222, 5331
BstZI	CGGCCG	1 cut(s)	63
Bsu36I	CCTNAGG	1 cut(s)	4140
BsuI	GTATCC	2 cut(s)	1378, 4920
BtgI	CCRYGG	3 cut(s)	903, 2108, 3858
BtrI	CACGTC	1 cut(s)	3014
BtsCI	GGATG	8 cut(s)	534, 579, 2308, 2460, 4007, 4179, 4609, 5254
BveI	ACCTGC	1 cut(s)	4039
Cac8I	GCNNGC	8 cut(s)	2353, 2796, 3413, 3759, 4001, 4483, 4942, 5254
CaiI	CAGNNNCTG	4 cut(s)	3128, 3292, 4291, 4846
CciI	TCATGA	3 cut(s)	159, 1504, 1900
CfoI	GCGC	2 cut(s)	2857, 4419
Cfr10I	RCCGGY	3 cut(s)	92, 741, 2760
Cfr13I	GGNCC	6 cut(s)	744, 2132, 2437, 2747, 3437, 3677
Cfr42I	CCGCGG	1 cut(s)	3861
CsiI	ACCWGGT	1 cut(s)	1362
CspAI	ACCGGT	1 cut(s)	741
DraI	TTTAAA	1 cut(s)	4969
DrdI	GACNNNNNNGTC	1 cut(s)	3770
DriI	GACNNNNNGTC	1 cut(s)	693
DseDI	GACNNNNNNGTC	1 cut(s)	3770
EaeI	YGGCCR	4 cut(s)	63, 3669, 3768, 4983
EagI	CGGCCG	1 cut(s)	63
Eam1104I	CTCTTC	6 cut(s)	907, 1317, 1681, 2216, 4156, 4358
Eam1105I	GACNNNNNGTC	1 cut(s)	693
EarI	CTCTTC	6 cut(s)	907, 1317, 1681, 2216, 4156, 4358
EclXI	CGGCCG	1 cut(s)	63
Eco105I	TACGTA	1 cut(s)	4159
Eco130I	CCWWGG	3 cut(s)	2108, 3238, 3542
Eco147I	AGGCCT	2 cut(s)	1053, 4138
Eco31I	GGTCTC	1 cut(s)	2653
Eco32I	GATATC	1 cut(s)	4442
Eco47I	GGWCC	4 cut(s)	744, 2132, 2437, 2747
Eco47III	AGCGCT	1 cut(s)	2856
Eco52I	CGGCCG	1 cut(s)	63
Eco81I	CCTNAGG	1 cut(s)	4140
EcoO109I	RGGNCCY	2 cut(s)	2747, 3677
EcoRI	GAATTC	1 cut(s)	4402
EcoRV	GATATC	1 cut(s)	4442
EcoT14I	CCWWGG	3 cut(s)	2108, 3238, 3542
EcoT22I	ATGCAT	3 cut(s)	1829, 2308, 4192
ErhI	CCWWGG	3 cut(s)	2108, 3238, 3542
FalI	AAGNNNNNCTT	6 cut(s)	904, 936, 1055, 1087, 4428, 4460
FaqI	GGGAC	7 cut(s)	29, 895, 1049, 2118, 3471, 4443, 5054
FauNDI	CATATG	2 cut(s)	513, 2827
FbaI	TGATCA	4 cut(s)	1530, 1908, 2122, 5164
FblI	GTMKAC	4 cut(s)	4270, 5155, 5221, 5330
FokI	GGATG	8 cut(s)	521, 566, 2315, 2447, 3994, 4166, 4616, 5261
GlaI	GCGC	2 cut(s)	2856, 4418
GsaI	CCCAGC	2 cut(s)	3180, 4702
GsuI	CTGGAG	3 cut(s)	1476, 2504, 2823
HaeII	RGCGCY	1 cut(s)	2858
HhaI	GCGC	2 cut(s)	2857, 4419
Hin1I	GRCGYC	1 cut(s)	4509
Hin6I	GCGC	2 cut(s)	2855, 4417
HinP1I	GCGC	2 cut(s)	2855, 4417
HincII	GTYRAC	2 cut(s)	2191, 2756
HindII	GTYRAC	2 cut(s)	2191, 2756
HindIII	AAGCTT	2 cut(s)	2528, 4829
HphI	GGTGA	8 cut(s)	130, 221, 546, 1328, 3635, 4253, 4273, 4775
Hpy99I	CGWCG	3 cut(s)	112, 3015, 4514
HpyCH4IV	ACGT	9 cut(s)	2815, 3013, 3994, 4158, 4176, 4273, 4509, 4773, 5001
HpyF10VI	GCNNNNNNNGC	9 cut(s)	62, 1775, 2804, 2831, 3359, 3577, 3737, 3767, 4946
HpySE526I	ACGT	9 cut(s)	2815, 3013, 3994, 4158, 4176, 4273, 4509, 4773, 5001
Hsp92I	GRCGYC	1 cut(s)	4509
HspAI	GCGC	2 cut(s)	2855, 4417
Kpn2I	TCCGGA	1 cut(s)	1270
Ksp22I	TGATCA	4 cut(s)	1530, 1908, 2122, 5164
KspI	CCGCGG	1 cut(s)	3861
MabI	ACCWGGT	1 cut(s)	1362
MaeII	ACGT	9 cut(s)	2815, 3013, 3994, 4158, 4176, 4273, 4509, 4773, 5001
MfeI	CAATTG	2 cut(s)	716, 2369
MflI	RGATCY	8 cut(s)	491, 3206, 3874, 4378, 4532, 4634, 5285, 5345
MhlI	GDGCHC	4 cut(s)	223, 2343, 3433, 4554
MlsI	TGGCCA	2 cut(s)	3671, 4985
MluNI	TGGCCA	2 cut(s)	3671, 4985
MlyI	GAGTC	5 cut(s)	176, 1271, 2030, 2233, 2482
MmeI	TCCRAC	5 cut(s)	159, 1783, 2919, 2971, 5047
Mox20I	TGGCCA	2 cut(s)	3671, 4985
Mph1103I	ATGCAT	3 cut(s)	1829, 2308, 4192
MroI	TCCGGA	1 cut(s)	1270
MroXI	GAANNNNTTC	5 cut(s)	851, 2456, 4227, 4581, 5090
MscI	TGGCCA	2 cut(s)	3671, 4985
MslI	CAYNNNNRTG	9 cut(s)	277, 2107, 2262, 2406, 2586, 2699, 4218, 5033, 5304
Msp20I	TGGCCA	2 cut(s)	3671, 4985
MspA1I	CMGCKG	2 cut(s)	3860, 4702
MspCI	CTTAAG	3 cut(s)	1099, 2137, 2894
MunI	CAATTG	2 cut(s)	716, 2369
Mva1269I	GAATGC	2 cut(s)	1829, 3178
MvnI	CGCG	2 cut(s)	3860, 4417
MwoI	GCNNNNNNNGC	9 cut(s)	62, 1775, 2804, 2831, 3359, 3577, 3737, 3767, 4946
NciI	CCSGG	2 cut(s)	503, 4499
NcoI	CCATGG	1 cut(s)	2108
NdeI	CATATG	2 cut(s)	513, 2827
NheI	GCTAGC	1 cut(s)	3757
NlaIV	GGNNCC	7 cut(s)	411, 901, 1590, 2134, 3595, 5055, 5311
NmuCI	GTSAC	5 cut(s)	227, 367, 1760, 4471, 5035
NsiI	ATGCAT	3 cut(s)	1829, 2308, 4192
NspI	RCATGY	6 cut(s)	319, 1580, 2064, 2355, 3329, 3415
NspV	TTCGAA	3 cut(s)	1381, 3618, 4585
OliI	CACNNNNGTG	1 cut(s)	2406
PaeI	GCATGC	2 cut(s)	2355, 3415
PagI	TCATGA	3 cut(s)	159, 1504, 1900
PceI	AGGCCT	2 cut(s)	1053, 4138
PciI	ACATGT	2 cut(s)	315, 2060
PcsI	WCGNNNNNNNCGW	1 cut(s)	3010
PctI	GAATGC	2 cut(s)	1829, 3178
PdmI	GAANNNNTTC	5 cut(s)	851, 2456, 4227, 4581, 5090
PflFI	GACNNNGTC	1 cut(s)	2751
PflMI	CCANNNNNTGG	3 cut(s)	178, 1032, 4291
PfoI	TCCNGGA	1 cut(s)	1453
PinAI	ACCGGT	1 cut(s)	741
PleI	GAGTC	5 cut(s)	176, 1270, 2030, 2232, 2481
PpsI	GAGTC	5 cut(s)	176, 1270, 2030, 2232, 2481
Ppu21I	YACGTR	2 cut(s)	4159, 4274
PpuMI	RGGWCCY	1 cut(s)	2747
PscI	ACATGT	2 cut(s)	315, 2060
PshBI	ATTAAT	3 cut(s)	360, 3708, 4065
PsiI	TTATAA	2 cut(s)	3551, 3753
Psp1406I	AACGTT	1 cut(s)	4773
Psp5II	RGGWCCY	1 cut(s)	2747
PspFI	CCCAGC	2 cut(s)	3176, 4698
PspN4I	GGNNCC	7 cut(s)	411, 901, 1590, 2134, 3595, 5055, 5311
PspPI	GGNCC	6 cut(s)	744, 2132, 2437, 2747, 3437, 3677
PspPPI	RGGWCCY	1 cut(s)	2747
PstI	CTGCAG	5 cut(s)	2201, 4036, 4483, 4851, 5044
PstNI	CAGNNNCTG	4 cut(s)	3128, 3292, 4291, 4846
PsuI	RGATCY	8 cut(s)	491, 3206, 3874, 4378, 4532, 4634, 5285, 5345
PsyI	GACNNNGTC	1 cut(s)	2751
PvuII	CAGCTG	1 cut(s)	4702
RseI	CAYNNNNRTG	9 cut(s)	277, 2107, 2262, 2406, 2586, 2699, 4218, 5033, 5304
SacII	CCGCGG	1 cut(s)	3861
Sau96I	GGNCC	6 cut(s)	744, 2132, 2437, 2747, 3437, 3677
SbfI	CCTGCAGG	1 cut(s)	4483
ScaI	AGTACT	2 cut(s)	3557, 5193
SchI	GAGTC	5 cut(s)	176, 1271, 2030, 2233, 2482
SdaI	CCTGCAGG	1 cut(s)	4483
SduI	GDGCHC	4 cut(s)	223, 2343, 3433, 4554
SexAI	ACCWGGT	1 cut(s)	1362
SfcI	CTRYAG	8 cut(s)	2197, 4015, 4032, 4236, 4453, 4479, 4847, 5040
Sfr303I	CCGCGG	1 cut(s)	3861
SfuI	TTCGAA	3 cut(s)	1381, 3618, 4585
SgrBI	CCGCGG	1 cut(s)	3861
SinI	GGWCC	4 cut(s)	744, 2132, 2437, 2747
SmiMI	CAYNNNNRTG	9 cut(s)	277, 2107, 2262, 2406, 2586, 2699, 4218, 5033, 5304
SnaBI	TACGTA	1 cut(s)	4159
SpeI	ACTAGT	1 cut(s)	4105
SphI	GCATGC	2 cut(s)	2355, 3415
Sse8387I	CCTGCAGG	1 cut(s)	4483
SseBI	AGGCCT	2 cut(s)	1053, 4138
SsiI	CCGC	8 cut(s)	53, 325, 1783, 2130, 2326, 3343, 3858, 3860
SspI	AATATT	1 cut(s)	4046
StuI	AGGCCT	2 cut(s)	1053, 4138
StyI	CCWWGG	3 cut(s)	2108, 3238, 3542
TaiI	ACGT	9 cut(s)	2818, 3016, 3997, 4161, 4179, 4276, 4512, 4776, 5004
TaqI	TCGA	9 cut(s)	129, 1381, 1446, 2014, 3010, 3618, 4512, 4585, 5064
TaqII	GACCGA	2 cut(s)	93, 4459
TatI	WGTACW	6 cut(s)	524, 928, 2057, 2080, 3555, 5191
TauI	GCSGC	3 cut(s)	56, 327, 1786
TseFI	GTSAC	5 cut(s)	227, 367, 1760, 4471, 5035
Tsp45I	GTSAC	5 cut(s)	227, 367, 1760, 4471, 5035
TspGWI	ACGGA	4 cut(s)	2630, 3367, 3586, 4966
Tth111I	GACNNNGTC	1 cut(s)	2751
Van91I	CCANNNNNTGG	3 cut(s)	178, 1032, 4291
Vha464I	CTTAAG	3 cut(s)	1099, 2137, 2894
VneI	GTGCAC	1 cut(s)	3429
VpaK11BI	GGWCC	4 cut(s)	744, 2132, 2437, 2747
VspI	ATTAAT	3 cut(s)	360, 3708, 4065
XbaI	TCTAGA	3 cut(s)	3097, 3466, 4853
XceI	RCATGY	6 cut(s)	319, 1580, 2064, 2355, 3329, 3415
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3245, 4271
XmaJI	CCTAGG	1 cut(s)	3542
XmiI	GTMKAC	4 cut(s)	4270, 5155, 5221, 5330
XmnI	GAANNNNTTC	5 cut(s)	851, 2456, 4227, 4581, 5090
ZraI	GACGTC	1 cut(s)	4510
ZrmI	AGTACT	2 cut(s)	3557, 5193
Zsp2I	ATGCAT	3 cut(s)	1829, 2308, 4192

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.