Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008435

Physical Location & Seq

Genomic Coordinates Forward (+)

6679460 .. 6706793

Gene Structure 27334 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1055600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4551 bp

ATGGCTGATAAACTCGAAGGATTTGATACCAGAGCAGAAAATGAATACTGTGACGGAATTAAAAGAGGCTTGGTTCATAAGGAGAGTTTGGTGAGGAAGCAGTCGTTGCATATGCTCAAGACAGCACTGGGCATAAATGAGTCTGGCCAAGCTCAGTCCTTCTCCGGTGGTGTTTCGGAGGCTGAAAATCAGTCTCGTGGTGACAGTGGCGGTAGCGGTGGTGGCATGACAAAGAGGGAGCTTTGGGCTGATAAGGAAGCCAAGTCATTGGGTGTTGGGAAAATGTGTAGCCCGGTTGAGTCTTTTCTGAACAGCCGGCAGAAGTGGGAGGCATTTGTACTGCTGTATGAAATGCTAGAAGAGTATGGCACTCATTTGGTTGAAGCAGCTTGGAACTTCCAGATATCTCTGTTGCTTCAATATTCTACCACACAAAGTAATGGTATTGTGGCAAGCTCTGTCAGTGGAGAAGTACTTCATCAAAACCTGATCGAAAGCCCAGGTGAAAGCTTTAGTTGGTTAGCAATTTTGTGGGAACGGGGTTTTCATCACGATAATCCTCAAGTTAGATGCTTGATCATGCAGTCATTTTTGGGCATTGATTGGAAGAATTATGGAAATCATGCAAAATCAGTGCCTAGATCTTTTGTTCTGGGTCCACTGATGGAAGGGCTAAATGACCCTGTGCACCACAAAGAATTTGGTGTAAAAGGACTATACTCCTCAGCGACGATTGAAGGTGCAGCTAGGTTTCTATGCCAGTATACAAGTTGCCTAAATGCGAGGACGTGCATTGCATTTCTGATCAATCTAGCACGCACTGCTAAGGTGCAATCATTTGGTAGAGTTGGATTAATGTGTCTTGCTGAATGCATTTCTTCAGCTGCTTGTCAAGTTCGTACAGATAATAATGAAAGTGAAGGACAGTGGCTTGAAGATGTGATTCCTGGCATGATCCAAGTGGAAAGTGTTCCCAATGACAAAGTTGTTCTACTTGATGCACTGAGATTTCTTATCGAGAGCAGCAAACAACATTTCAATCCTAATTATCGCCTTCGAGTTTGTGAGAAAATCCTGGAGTCCGCTACCTCAGTGGTGTGTGCATGTGATCTCCCTCTTGACATACTTATGCACTTCATTTCAACATTTCCTTGGGAATTTACCGACTTCGGTGGTTCCTTAAGAGTGAAATTACAAGAATGGCTTATGGGGTGTAGTAAGAAGAATTGTTATGGCAACTGCTGCAGTACTGAGACGAAGCTTCTAAAGGGTCTCCATGAATTCCCCAGTAGTTTTACAGTTCATTATTCAGTTGATAGTTCCATCACTTTTGATGATGAAGACTTGGAATCCTGGGAATTTGAAGCGAAGCGGTGGGCACGAGTGCTTTTTCTTACATGCCAGGAGGAATATCAATTGATACCTACACTGATGTTTATTCAAACTCATGCTCTTGGTCTCTGCCAAGAAAACAACAATTTGGATCAAATTCCTGTGAAGTTTCTCATTGTCACTTTGAGCTTGGTGCGGGAGCTTCAGATGATGCAAGACAGAGTCGCTGAATATAGATCTACAGTTAGAACCAAATCAGAATCTCGTGCGCTTGAATTAATGGATCAATTTGGTCATCCAGATGCTCTTAACTTATACCAGAAGTTTACCAATGTTTTTATTGTTATAATGAAGGAGTTGGTGTCTTTGGCAAACTTGTCCTGTTCTATATTCTCACATGCCAATACCACTAAGATGGCAGATGCCAGCCTTCCTGGTTCAGTGAAAGGAAAACTTGGAGGCCCTAGTCAACGCCGTTTGTCATCCTCTACCACCACTGCTGTGTTGCAAGCGATAATTTCCATGAAGGCTCTTGCAACTATCTCATCATGGTGTGCACAATTTATAGCTGATGCATCACTTGATCTTGCTTTTAATTTTATGTGGGAATTCTACTGGAAAACTGTTTCATCTCCAGTTACTGATTCAGAGACTGGGGCGGAAATAAGTCTTGCAGCATATGAAGCATTAGCTCCTGCTCTCACAGCACTAGTGTCTATGTTTTCTCCTAAATCGTTGGATCTTGTCAAGAACAATGGCAATCTGTTTCTATCAGATGGAAAACCTCTGTTAGATTCCTTGGTTCTTTCTTTTCTTCAGAACATAAATAATCTCCTAGCAATTGGAGTATTTGTACGGACTCGACGTGCAGTTTTAATGAATTGGAAGTGGATTTGCCTAGAATCTCTACTGTCAATTCCTTGCTATGCTTTAAAAAAAGGGCTTCATTTAGAGGACAATACTTTTTTGCTCTCAGGTGATACTCTCAGATTGATTTTTACTGATCTTCTTGAGAGCCTAGAGAATGCTGGAGAAAATTCTGTTCTACCAATGCTCAGATCTGTCCGATTAGTTTTGGGCCTATTGTCCGAGGGAAAGTCTGGTTCACTTGTCTATTCATGTGATGGGGTGGATGCCCAGATGATGTGGCAGTTGGTTCAGTCTTCTTGGATTTTGCATGTCAGTTGTAACAAGAGGAAGGTTGCACCTATTGCTGCACTTTTGTCTTCTGTTCTGCATTCATCCCTATTTAGTGATGAGAGCATGCATATGAATGACAATGCACCTGGGCCTCTGAAGTGGTTTGTTGAGAAAATTCTGGAGGAAGGCACAAAAAGTCCCCGCACAATTCGTCTTGCTGCATTACACTTAACGGGCCTCTTTCTATCATATCCTAGGATTATAAAGTACTATGTGAAGGAGTTGAAGCTGCTGTCACTACATGGTTCTGTTGCATTCGATGAGGACTTTGAAGGTGAATTAGCTGACAATCATGATACAAGGACTGAAGTTTCTTTATTGGCGAAAGGCCCTGATCCTGAGCTTACCAAAGAATTTATCAACACAGAATTGTATGCACGTGCCTCTGTTGCTGTTCTGTTTTACAAGCTAGCAGACTTGTCTGATATGGTGGGATCACCAAATGAAAATGAAGATTGCCATGCAGCTTTGGAATCTGGAAAAATATTTTTGCTTGAGCTTCTAGATTCTGCGGTAAACGACAAGGATCTTTCGAAAGAGCTGTATAAGAAATACAGTGCAATCCACAGGCGTAAGGTACGTGCGTGGCAAATGATATGTATTTTGTCACGTTTCATATGTCAAGATATAGTTTCTGAAGTTTCACGTTGCCTGCACATATGCCTTTATATGAACAATTTACCTGCAGTTCGTCAATACTTGGAAACATTCGCAATTAATATGTACTTGAAGTTCCCATCATTGGTTGGGGAGCAACTAGTCCCTGTATTAAGAGATTATGAGATGAGGCCTCAGGCGCTCTCTTCATATGTATTTATAGCAGCCAATGTCATCCTCCATGCATCTCAAGCTGTTCAAGACAAGCATTTGAATGAATTACTTCCTCCTATATTTCCACTACTAACATCCCATCACCACAGTTTGCGTGGTTTCGCCCAGTTATTGGTTTACCAAGTTCTCTGCAAATTGTTTCCTCCATTGGACTCTAGGGCCTCTGGAACTATGACTTTAGAGAAGAGGTGTTTTGAGGATTTGAAATCCTACCTGGCAAAGAACTATGATTGTGTGCGCCTACGAGCATCAATGGGAGGATTTCTTGATGCCTACAGTCCCAGTAGCTCTGTCACTCCTGCTGGGATTTTCATCAACCGGGTTGAGGCACTCGAGTTTGAATGTGTACCAGTGTCACTTACGGAGCAAGTGCTCAACTTTTTAAATGATGTCAGGGAAGATCTTCGGAGTTCAATGGCAAAAGATGCTGCGGCTATTAAGAATGAAAGCTTAAGATCTGATGAAGATCAAAATTGCATAGCAATACCATCTAATGCAAATGAAGGAAACTCACATACCCAGCAGCCCAAAGATATTTCACTGGATTTTCAAAAAAAGATTACCCTCTCTAAACATGAGAAGCATGATATAGCCGTAAATTCCTTCTTTGGCAACCAAGACACTTACAAACCACTTGAAGAAATGGAGAAGGAAGATAAGCTTCTTGCTCAGGTGCTGCAGTCTAGAAGTCAGGCAATGGAAAGAGAGAGAGCAAGCAGACAACAACTCATTCTTGTGGCATCTCTTCTTGATCGCATACCTAACCTTGCTGGCCTGGCACGGACTTGTGAGGTGTTTAAGGTGTCGGGTTTGGCTGTTGCGGATGCAAACGTAGTACATGACAAACAGTTCCAGCTCATCAGTGTGACTGCAGAAAAGTGGGTTCCTATCATTGAAGTCCCAGTGGATAGTTTGAAGGTCTTCCTTGAGAGAAAGAAACGAGAAGGCTTTTCAATTTTGGGACTGGAGCAAACTGCAAATAGTGTACCGCTTGACCAGTATAGCTTTCCTAAAAAGACGGTACTAGTCCTTGGGCGTGAAAAGGAAGGTATACCGGTGGATATCATTCATATTCTGGATGCTTGCATTGAGATTCCACAGTTGGGAGTTGTTAGATCTTTAAATGTTCATGTCAGCGGTGCCATTGCGCTGTGGGAGTATACTCGACAGCAGAGATCATCCCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000451 GO:0000453 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006355 GO:0006357 GO:0006364 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008173 GO:0008175 GO:0008649 GO:0008757 GO:0009451 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016072 GO:0016423 GO:0016435 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0022613 GO:0030488 GO:0031167 GO:0031323 GO:0031326 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070039 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:0140102 GO:1901360 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SpoU_methylase

Protein Analysis

1517

Amino Acids

169.64

Weight (kDa)

5.83

Isoelectric Point (pI)

42.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SpoU_methylase	PF00588	1364 - 1506	2e-26	SpoU rRNA Methylase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008435)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17610 AT4G17610 AT4G17610
fragaria_vesca	FvH4_5g20370 FvH4_5g20370 FvH4_5g20370 FvH4_5g20370 FvH4_5g20370
malus_domestica	MD04G1055600.v1.1
prunus_persica	Prupe.5G055400_v2.0.a1 Prupe.5G055400_v2.0.a1
pyrus_communis	pycom04g04890
rosa_chinensis	RchiOBHm_Chr7g0205631
rosa_laevigata	RLG00000003369
rosa_multiflora	Rmu_ssc0000482.1_g000025
rosa_roxburghii	Rroxscaffold_3G00252130
rosa_rugosa	Rorug07G0092300
rosa_samantha	Rh7AG223100 Rh7AG230100 Rh7BG218100 Rh7CG236300 Rh7DG230000
rosa_wichuraiana	Rw7G019240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1679, 2735
Acc36I	ACCTGC	1 cut(s)	3223
AccB1I	GGYRCC	1 cut(s)	4502
AccB7I	CCANNNNNTGG	1 cut(s)	3475
AccI	GTMKAC	3 cut(s)	764, 4414, 4523
AclWI	GGATC	7 cut(s)	949, 1491, 1623, 2079, 2861, 2974, 3066
AcoI	YGGCCR	1 cut(s)	145
AcuI	CTGAAG	6 cut(s)	864, 1520, 2132, 2648, 2859, 3189
AcvI	CACGTG	1 cut(s)	2912
AfiI	CCNNNNNNNGG	4 cut(s)	3274, 3475, 3688, 4466
AflII	CTTAAG	2 cut(s)	1180, 3814
AgeI	ACCGGT	1 cut(s)	4417
AhlI	ACTAGT	3 cut(s)	2041, 3289, 4387
AjiI	CACGTC	2 cut(s)	789, 2198
AjnI	CCWGG	9 cut(s)	499, 946, 1074, 1352, 1401, 1765, 2617, 3576, 4137
AleI	CACNNNNGTG	1 cut(s)	1832
Alw21I	GWGCWC	3 cut(s)	690, 1891, 3738
Alw26I	GTCTC	5 cut(s)	198, 1247, 1277, 1463, 1976
Alw44I	GTGCAC	2 cut(s)	686, 1887
AlwI	GGATC	7 cut(s)	949, 1491, 1623, 2079, 2861, 2974, 3066
AlwNI	CAGNNNCTG	3 cut(s)	1973, 1985, 4039
Ama87I	CYCGRG	1 cut(s)	3695
AoxI	GGCC	9 cut(s)	145, 1792, 2410, 2621, 2707, 2860, 3320, 3522, 4135
ApaLI	GTGCAC	2 cut(s)	686, 1887
AseI	ATTAAT	3 cut(s)	854, 1610, 3249
AsiGI	ACCGGT	1 cut(s)	4417
Asp700I	GAANNNNTTC	5 cut(s)	474, 969, 3411, 3765, 4283
AspA2I	CCTAGG	1 cut(s)	2726
AspLEI	GCGC	4 cut(s)	1603, 3331, 3603, 4513
AspS9I	GGNCC	7 cut(s)	656, 1793, 2410, 2621, 2707, 2861, 3522
AsuC2I	CCSGG	2 cut(s)	293, 3683
AsuHPI	GGTGA	7 cut(s)	103, 212, 515, 2321, 2819, 2961, 3437
AsuII	TTCGAA	1 cut(s)	3065
AsuNHI	GCTAGC	1 cut(s)	2941
AvaI	CYCGRG	1 cut(s)	3695
AvaII	GGWCC	1 cut(s)	656
AvrII	CCTAGG	1 cut(s)	2726
AxyI	CCTNAGG	1 cut(s)	3324
BaeGI	GKGCMC	3 cut(s)	690, 1381, 1891
BaeI	ACNNNNGTAYC	2 cut(s)	3101, 3134
BalI	TGGCCA	1 cut(s)	147
BanI	GGYRCC	1 cut(s)	4502
BauI	CACGAG	3 cut(s)	195, 1380, 1596
BbrPI	CACGTG	1 cut(s)	2912
BbsI	GAAGAC	4 cut(s)	1347, 2487, 2550, 4276
Bbv12I	GWGCWC	3 cut(s)	690, 1891, 3738
BbvCI	CCTCAGC	1 cut(s)	724
BccI	CCATC	8 cut(s)	658, 1331, 1741, 2102, 2450, 3277, 3450, 3859
BceAI	ACGGC	2 cut(s)	1791, 3941
BciT130I	CCWGG	9 cut(s)	501, 948, 1076, 1354, 1403, 1767, 2619, 3578, 4139
BclI	TGATCA	2 cut(s)	576, 804
BcnI	CCSGG	2 cut(s)	293, 3683
BcoDI	GTCTC	5 cut(s)	198, 1247, 1277, 1463, 1976
BcuI	ACTAGT	3 cut(s)	2041, 3289, 4387
BfmI	CTRYAG	6 cut(s)	1243, 1572, 3216, 3637, 4040, 4233
BfoI	RGCGCY	1 cut(s)	3332
BfrI	CTTAAG	2 cut(s)	1180, 3814
BfuAI	ACCTGC	1 cut(s)	3223
BglII	AGATCT	6 cut(s)	641, 1568, 2390, 3763, 3818, 4478
BlnI	CCTAGG	1 cut(s)	2726
BmcAI	AGTACT	3 cut(s)	474, 1249, 2741
Bme18I	GGWCC	1 cut(s)	656
BmeT110I	CYCGRG	1 cut(s)	3695
BmgBI	CACGTC	2 cut(s)	789, 2198
BmgT120I	GGNCC	7 cut(s)	656, 1793, 2410, 2621, 2707, 2861, 3522
BmiI	GGNNCC	4 cut(s)	657, 1177, 4248, 4504
BmrI	ACTGGG	7 cut(s)	137, 1281, 1995, 3463, 3639, 4259, 4540
BmtI	GCTAGC	1 cut(s)	2945
BmuI	ACTGGG	7 cut(s)	137, 1281, 1995, 3463, 3639, 4259, 4540
BpiI	GAAGAC	4 cut(s)	1347, 2487, 2550, 4276
BpmI	CTGGAG	5 cut(s)	1097, 1950, 2382, 2672, 4349
Bpu10I	CCTNAGC	4 cut(s)	724, 825, 2871, 4032
Bpu14I	TTCGAA	1 cut(s)	3065
BpuEI	CTTGAG	6 cut(s)	101, 546, 2363, 3047, 3363, 4310
BpuMI	CCSGG	2 cut(s)	293, 3683
BsaAI	YACGTR	2 cut(s)	2912, 3113
BsaBI	GATNNNNATC	2 cut(s)	3674, 3828
BsaI	GGTCTC	2 cut(s)	1277, 1463
BsaJI	CCNNGG	8 cut(s)	499, 1151, 1353, 2130, 2421, 2618, 2726, 4393
BsaWI	WCCGGW	2 cut(s)	164, 4417
BsaXI	ACNNNNNCTCC	4 cut(s)	1679, 1709, 2169, 2199
Bsc4I	CCNNNNNNNGG	4 cut(s)	3274, 3475, 3688, 4466
Bse118I	RCCGGY	2 cut(s)	315, 4417
Bse21I	CCTNAGG	1 cut(s)	3324
Bse3DI	GCAATG	3 cut(s)	792, 4065, 4506
Bse8I	GATNNNNATC	2 cut(s)	3674, 3828
BseBI	CCWGG	9 cut(s)	501, 948, 1076, 1354, 1403, 1767, 2619, 3578, 4139
BseDI	CCNNGG	8 cut(s)	499, 1151, 1353, 2130, 2421, 2618, 2726, 4393
BseGI	GGATG	9 cut(s)	1627, 1814, 2470, 2573, 3363, 3437, 4192, 4447, 4541
BseJI	GATNNNNATC	2 cut(s)	3674, 3828
BseLI	CCNNNNNNNGG	4 cut(s)	3274, 3475, 3688, 4466
BseMI	GCAATG	3 cut(s)	792, 4065, 4506
BseRI	GAGGAG	1 cut(s)	712
BseSI	GKGCMC	3 cut(s)	690, 1381, 1891
BseYI	CCCAGC	2 cut(s)	3665, 3882
BsgI	GTGCAG	4 cut(s)	762, 2220, 2532, 3170
BshFI	GGCC	9 cut(s)	147, 1794, 2412, 2623, 2709, 2862, 3322, 3524, 4137
BshNI	GGYRCC	1 cut(s)	4502
BshTI	ACCGGT	1 cut(s)	4417
BsiHKAI	GWGCWC	3 cut(s)	690, 1891, 3738
BsiHKCI	CYCGRG	1 cut(s)	3695
BsiSI	CCGG	5 cut(s)	165, 293, 316, 3682, 4418
BslFI	GGGAC	5 cut(s)	2655, 3278, 3627, 4247, 4338
BslI	CCNNNNNNNGG	4 cut(s)	3274, 3475, 3688, 4466
BsmAI	GTCTC	5 cut(s)	198, 1247, 1277, 1463, 1976
BsmBI	CGTCTC	1 cut(s)	1247
BsmFI	GGGAC	5 cut(s)	2655, 3278, 3627, 4247, 4338
BsmI	GAATGC	4 cut(s)	875, 2362, 2569, 2786
BsnI	GGCC	9 cut(s)	147, 1794, 2412, 2623, 2709, 2862, 3322, 3524, 4137
Bso31I	GGTCTC	2 cut(s)	1277, 1463
BsoBI	CYCGRG	1 cut(s)	3695
Bsp119I	TTCGAA	1 cut(s)	3065
Bsp1286I	GDGCHC	4 cut(s)	690, 1381, 1891, 3738
BspANI	GGCC	9 cut(s)	147, 1794, 2412, 2623, 2709, 2862, 3322, 3524, 4137
BspHI	TCATGA	1 cut(s)	2824
BspLI	GGNNCC	4 cut(s)	657, 1177, 4248, 4504
BspMAI	CTGCAG	4 cut(s)	1247, 3220, 4044, 4237
BspMI	ACCTGC	1 cut(s)	3223
BspOI	GCTAGC	1 cut(s)	2945
BspPI	GGATC	7 cut(s)	949, 1491, 1623, 2079, 2861, 2974, 3066
BspT104I	TTCGAA	1 cut(s)	3065
BspT107I	GGYRCC	1 cut(s)	4502
BspTI	CTTAAG	2 cut(s)	1180, 3814
BspTNI	GGTCTC	2 cut(s)	1277, 1463
BsrDI	GCAATG	3 cut(s)	792, 4065, 4506
BsrFI	RCCGGY	2 cut(s)	315, 4417
BssAI	RCCGGY	2 cut(s)	315, 4417
BssECI	CCNNGG	8 cut(s)	499, 1151, 1353, 2130, 2421, 2618, 2726, 4393
BssNAI	GTATAC	3 cut(s)	765, 4415, 4524
BssSI	CACGAG	3 cut(s)	195, 1380, 1596
BssT1I	CCWWGG	4 cut(s)	1151, 2130, 2726, 4393
Bst1107I	GTATAC	3 cut(s)	765, 4415, 4524
Bst2BI	CACGAG	3 cut(s)	195, 1380, 1596
Bst2UI	CCWGG	9 cut(s)	501, 948, 1076, 1354, 1403, 1767, 2619, 3578, 4139
Bst6I	CTCTTC	4 cut(s)	354, 3340, 3542, 4113
BstAFI	CTTAAG	2 cut(s)	1180, 3814
BstAPI	GCANNNNNTGC	5 cut(s)	106, 821, 1242, 2543, 3788
BstBAI	YACGTR	2 cut(s)	2912, 3113
BstBI	TTCGAA	1 cut(s)	3065
BstF5I	GGATG	9 cut(s)	1627, 1814, 2470, 2573, 3363, 3437, 4192, 4447, 4541
BstH2I	RGCGCY	1 cut(s)	3332
BstHHI	GCGC	4 cut(s)	1603, 3331, 3603, 4513
BstMAI	GTCTC	5 cut(s)	198, 1247, 1277, 1463, 1976
BstNI	CCWGG	9 cut(s)	501, 948, 1076, 1354, 1403, 1767, 2619, 3578, 4139
BstNSI	RCATGY	5 cut(s)	1107, 1401, 1733, 2513, 2599
BstSFI	CTRYAG	6 cut(s)	1243, 1572, 3216, 3637, 4040, 4233
BstSLI	GKGCMC	3 cut(s)	690, 1381, 1891
BstV2I	GAAGAC	4 cut(s)	1347, 2487, 2550, 4276
BstX2I	RGATCY	8 cut(s)	641, 1568, 2071, 2390, 3058, 3763, 3818, 4478
BstXI	CCANNNNNNTGG	2 cut(s)	268, 1747
BstYI	RGATCY	8 cut(s)	641, 1568, 2071, 2390, 3058, 3763, 3818, 4478
BstZ17I	GTATAC	3 cut(s)	765, 4415, 4524
Bsu36I	CCTNAGG	1 cut(s)	3324
BsuRI	GGCC	9 cut(s)	147, 1794, 2412, 2623, 2709, 2862, 3322, 3524, 4137
BtrI	CACGTC	2 cut(s)	789, 2198
BtsCI	GGATG	9 cut(s)	1627, 1814, 2470, 2573, 3363, 3437, 4192, 4447, 4541
BtsI	GCAGTG	2 cut(s)	819, 1827
BveI	ACCTGC	1 cut(s)	3223
CaiI	CAGNNNCTG	3 cut(s)	1973, 1985, 4039
CciI	TCATGA	1 cut(s)	2824
CfoI	GCGC	4 cut(s)	1603, 3331, 3603, 4513
Cfr10I	RCCGGY	2 cut(s)	315, 4417
Cfr13I	GGNCC	7 cut(s)	656, 1793, 2410, 2621, 2707, 2861, 3522
CspAI	ACCGGT	1 cut(s)	4417
DraI	TTTAAA	3 cut(s)	2265, 3747, 4485
EaeI	YGGCCR	1 cut(s)	145
Eam1104I	CTCTTC	4 cut(s)	354, 3340, 3542, 4113
EarI	CTCTTC	4 cut(s)	354, 3340, 3542, 4113
EciI	GGCGGA	1 cut(s)	2006
Eco130I	CCWWGG	4 cut(s)	1151, 2130, 2726, 4393
Eco147I	AGGCCT	1 cut(s)	3322
Eco31I	GGTCTC	2 cut(s)	1277, 1463
Eco32I	GATATC	2 cut(s)	405, 4426
Eco47I	GGWCC	1 cut(s)	656
Eco57I	CTGAAG	6 cut(s)	864, 1520, 2132, 2648, 2859, 3189
Eco72I	CACGTG	1 cut(s)	2912
Eco81I	CCTNAGG	1 cut(s)	3324
Eco88I	CYCGRG	1 cut(s)	3695
EcoO109I	RGGNCCY	3 cut(s)	1793, 2861, 3522
EcoRI	GAATTC	2 cut(s)	1280, 1940
EcoRII	CCWGG	9 cut(s)	499, 946, 1074, 1352, 1401, 1765, 2617, 3576, 4137
EcoRV	GATATC	2 cut(s)	405, 4426
EcoT14I	CCWWGG	4 cut(s)	1151, 2130, 2726, 4393
EcoT22I	ATGCAT	4 cut(s)	875, 1909, 2601, 3376
ErhI	CCWWGG	4 cut(s)	1151, 2130, 2726, 4393
Esp3I	CGTCTC	1 cut(s)	1247
FalI	AAGNNNNNCTT	6 cut(s)	1188, 1220, 1770, 1802, 4008, 4040
FaqI	GGGAC	5 cut(s)	2655, 3278, 3627, 4247, 4338
FauI	CCCGC	2 cut(s)	1521, 2681
FauNDI	CATATG	6 cut(s)	111, 2011, 2601, 3149, 3191, 3340
FbaI	TGATCA	2 cut(s)	576, 804
FblI	GTMKAC	3 cut(s)	764, 4414, 4523
FokI	GGATG	9 cut(s)	1614, 1801, 2477, 2560, 3350, 3424, 4199, 4454, 4528
GlaI	GCGC	4 cut(s)	1602, 3330, 3602, 4512
GsaI	CCCAGC	2 cut(s)	3669, 3886
GsuI	CTGGAG	5 cut(s)	1097, 1950, 2382, 2672, 4349
HaeII	RGCGCY	1 cut(s)	3332
HaeIII	GGCC	9 cut(s)	147, 1794, 2412, 2623, 2709, 2862, 3322, 3524, 4137
HapII	CCGG	5 cut(s)	165, 293, 316, 3682, 4418
HhaI	GCGC	4 cut(s)	1603, 3331, 3603, 4513
Hin6I	GCGC	4 cut(s)	1601, 3329, 3601, 4511
HinP1I	GCGC	4 cut(s)	1601, 3329, 3601, 4511
HincII	GTYRAC	1 cut(s)	1802
HindII	GTYRAC	1 cut(s)	1802
HindIII	AAGCTT	4 cut(s)	508, 1259, 3811, 4022
HpaII	CCGG	5 cut(s)	165, 293, 316, 3682, 4418
HphI	GGTGA	7 cut(s)	103, 212, 515, 2321, 2819, 2961, 3437
Hpy99I	CGWCG	2 cut(s)	733, 2199
HpyCH4IV	ACGT	7 cut(s)	788, 2197, 2911, 3112, 3142, 3178, 4194
HpySE526I	ACGT	7 cut(s)	788, 2197, 2911, 3112, 3142, 3178, 4194
HspAI	GCGC	4 cut(s)	1601, 3329, 3601, 4511
KroI	GCCGGC	1 cut(s)	315
KroNI	GCCGGC	1 cut(s)	317
Ksp22I	TGATCA	2 cut(s)	576, 804
LmnI	GCTCC	6 cut(s)	238, 1531, 2029, 3283, 3727, 4330
MaeII	ACGT	7 cut(s)	788, 2197, 2911, 3112, 3142, 3178, 4194
MfeI	CAATTG	2 cut(s)	1415, 2172
MflI	RGATCY	8 cut(s)	641, 1568, 2071, 2390, 3058, 3763, 3818, 4478
MhlI	GDGCHC	4 cut(s)	690, 1381, 1891, 3738
MlsI	TGGCCA	1 cut(s)	147
MluNI	TGGCCA	1 cut(s)	147
MlyI	GAGTC	6 cut(s)	149, 308, 1088, 1563, 2185, 3509
MmeI	TCCRAC	2 cut(s)	829, 2049
Mox20I	TGGCCA	1 cut(s)	147
Mph1103I	ATGCAT	4 cut(s)	875, 1909, 2601, 3376
MroNI	GCCGGC	1 cut(s)	315
MroXI	GAANNNNTTC	5 cut(s)	474, 969, 3411, 3765, 4283
MscI	TGGCCA	1 cut(s)	147
Msp20I	TGGCCA	1 cut(s)	147
MspA1I	CMGCKG	2 cut(s)	884, 4500
MspCI	CTTAAG	2 cut(s)	1180, 3814
MspI	CCGG	5 cut(s)	165, 293, 316, 3682, 4418
MunI	CAATTG	2 cut(s)	1415, 2172
Mva1269I	GAATGC	4 cut(s)	875, 2362, 2569, 2786
MvaI	CCWGG	9 cut(s)	501, 948, 1076, 1354, 1403, 1767, 2619, 3578, 4139
NaeI	GCCGGC	1 cut(s)	317
NciI	CCSGG	2 cut(s)	293, 3683
NdeI	CATATG	6 cut(s)	111, 2011, 2601, 3149, 3191, 3340
NgoMIV	GCCGGC	1 cut(s)	315
NheI	GCTAGC	1 cut(s)	2941
NlaIV	GGNNCC	4 cut(s)	657, 1177, 4248, 4504
NmuCI	GTSAC	8 cut(s)	50, 200, 1510, 2766, 3138, 3655, 3717, 4228
NsiI	ATGCAT	4 cut(s)	875, 1909, 2601, 3376
NspI	RCATGY	5 cut(s)	1107, 1401, 1733, 2513, 2599
NspV	TTCGAA	1 cut(s)	3065
OliI	CACNNNNGTG	1 cut(s)	1832
PaeI	GCATGC	1 cut(s)	2599
PaeR7I	CTCGAG	1 cut(s)	3695
PagI	TCATGA	1 cut(s)	2824
PceI	AGGCCT	1 cut(s)	3322
PcsI	WCGNNNNNNNCGW	1 cut(s)	2194
PctI	GAATGC	4 cut(s)	875, 2362, 2569, 2786
PdiI	GCCGGC	1 cut(s)	317
PdmI	GAANNNNTTC	5 cut(s)	474, 969, 3411, 3765, 4283
PfeI	GAWTC	9 cut(s)	943, 1349, 1592, 1976, 2126, 2234, 3005, 3038, 4456
PflFI	GACNNNGTC	1 cut(s)	1553
PflMI	CCANNNNNTGG	1 cut(s)	3475
PfoI	TCCNGGA	1 cut(s)	1074
PinAI	ACCGGT	1 cut(s)	4417
PleI	GAGTC	6 cut(s)	148, 307, 1087, 1562, 2185, 3509
PmaCI	CACGTG	1 cut(s)	2912
PmlI	CACGTG	1 cut(s)	2912
PpsI	GAGTC	6 cut(s)	148, 307, 1087, 1562, 2185, 3509
Ppu21I	YACGTR	2 cut(s)	2912, 3113
PshBI	ATTAAT	3 cut(s)	854, 1610, 3249
PsiI	TTATAA	2 cut(s)	1679, 2735
Psp6I	CCWGG	9 cut(s)	499, 946, 1074, 1352, 1401, 1765, 2617, 3576, 4137
PspCI	CACGTG	1 cut(s)	2912
PspFI	CCCAGC	2 cut(s)	3665, 3882
PspGI	CCWGG	9 cut(s)	499, 946, 1074, 1352, 1401, 1765, 2617, 3576, 4137
PspN4I	GGNNCC	4 cut(s)	657, 1177, 4248, 4504
PspPI	GGNCC	7 cut(s)	656, 1793, 2410, 2621, 2707, 2861, 3522
PspXI	VCTCGAGB	1 cut(s)	3695
PsrI	GAACNNNNNNTAC	2 cut(s)	3248, 3280
PstI	CTGCAG	4 cut(s)	1247, 3220, 4044, 4237
PstNI	CAGNNNCTG	3 cut(s)	1973, 1985, 4039
PsuI	RGATCY	8 cut(s)	641, 1568, 2071, 2390, 3058, 3763, 3818, 4478
PsyI	GACNNNGTC	1 cut(s)	1553
PvuII	CAGCTG	1 cut(s)	884
Sau96I	GGNCC	7 cut(s)	656, 1793, 2410, 2621, 2707, 2861, 3522
ScaI	AGTACT	3 cut(s)	474, 1249, 2741
SchI	GAGTC	6 cut(s)	149, 308, 1088, 1563, 2185, 3509
SduI	GDGCHC	4 cut(s)	690, 1381, 1891, 3738
SfcI	CTRYAG	6 cut(s)	1243, 1572, 3216, 3637, 4040, 4233
Sfr274I	CTCGAG	1 cut(s)	3695
SfuI	TTCGAA	1 cut(s)	3065
SinI	GGWCC	1 cut(s)	656
SlaI	CTCGAG	1 cut(s)	3695
SmlI	CTYRAG	9 cut(s)	116, 561, 1180, 2342, 3026, 3378, 3695, 3814, 4289
SmoI	CTYRAG	9 cut(s)	116, 561, 1180, 2342, 3026, 3378, 3695, 3814, 4289
SpeI	ACTAGT	3 cut(s)	2041, 3289, 4387
SphI	GCATGC	1 cut(s)	2599
SseBI	AGGCCT	1 cut(s)	3322
SspI	AATATT	2 cut(s)	422, 3018
StuI	AGGCCT	1 cut(s)	3322
StyI	CCWWGG	4 cut(s)	1151, 2130, 2726, 4393
TaiI	ACGT	7 cut(s)	791, 2200, 2914, 3115, 3145, 3181, 4197
TaqI	TCGA	9 cut(s)	15, 492, 1017, 1057, 2194, 2790, 3065, 3696, 4528
TatI	WGTACW	6 cut(s)	337, 472, 1247, 2739, 3255, 4198
TauI	GCSGC	1 cut(s)	3797
TfiI	GAWTC	9 cut(s)	943, 1349, 1592, 1976, 2126, 2234, 3005, 3038, 4456
TseFI	GTSAC	8 cut(s)	50, 200, 1510, 2766, 3138, 3655, 3717, 4228
Tsp45I	GTSAC	8 cut(s)	50, 200, 1510, 2766, 3138, 3655, 3717, 4228
TspGWI	ACGGA	4 cut(s)	69, 2203, 3740, 4159
Tth111I	GACNNNGTC	1 cut(s)	1553
Van91I	CCANNNNNTGG	1 cut(s)	3475
Vha464I	CTTAAG	2 cut(s)	1180, 3814
VneI	GTGCAC	2 cut(s)	686, 1887
VpaK11BI	GGWCC	1 cut(s)	656
VspI	ATTAAT	3 cut(s)	854, 1610, 3249
XbaI	TCTAGA	2 cut(s)	3034, 4046
XceI	RCATGY	5 cut(s)	1107, 1401, 1733, 2513, 2599
XcmI	CCANNNNNNNNNTGG	2 cut(s)	698, 3455
XhoI	CTCGAG	1 cut(s)	3695
XmaJI	CCTAGG	1 cut(s)	2726
XmiI	GTMKAC	3 cut(s)	764, 4414, 4523
XmnI	GAANNNNTTC	5 cut(s)	474, 969, 3411, 3765, 4283
ZrmI	AGTACT	3 cut(s)	474, 1249, 2741
Zsp2I	ATGCAT	4 cut(s)	875, 1909, 2601, 3376

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.