Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0008435

Physical Location & Seq

Genomic Coordinates Forward (+)

47695269 .. 47716275

Gene Structure 21007 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003369

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5523 bp

ATGTCTACACAAGAAACCCACTCTATGGCTACTCTTATCACTACACTCTCCACCAGCTTCAGTCAAGTCCCTCCGGCGGCTGTTCCGGCCGTCTTGGATTGCATTTTGGCCTCTACCGGCGTGTCTCGGTCGTCGCTATTCGCTTCGCTTCTCGACACTTTCACCAAGCTCTCTGAGGGTGTCATGAAAAACGACCAGAAGTTGGACTCCCATGACTGTAACTATCTGGCATCGTTGGTTGGGGCACTTTGTCACCTTCTAAAGAAATTTGACACTAATCTTGGTGCTTTGGAATCATTTATATGGAAATGTTTTCTTCCATTGATAAAGATGGCACACATGTTCAGCCGCAACATGCTTAACCAGATTGCAGAATCGTTTATTAATGCTGTGACTGAGACAAACACCTGGGTAGTTGTGGAGGCAACACTGGTGCCAATTTTTCTAACATTAGTTGCTAGTTCTATTGGTATGCCTGAAAAGGAAGTATCAGATACTTTTCAGTTCATGTTGGGATCTAATGACCGGGTAAATCATATGGAAATGAGTACAAAAAACATCCTTTCGCCACCTGGATCACCTTCGCTGCCAATTTCATGCCACATCCTAACCTTAATGCTTGATGCTGTCCTTCAAAGCAAGAAAATAGCTGCCTCCTCAGAATCAATAATTGCAAATGGGTGTTTTGGTGATTATTTTACTGGTAAATTGACTTGGGGTCTTTGTAGCTTGACTGAGCAATTGCTTTTACAAAATTTGGAACACCGGTCCTGTGCTACTGCCTTTCTTCTCCCAATCATTTTCAAAGCATTTTCTTCTTATTGCTCTTTTCAAATCTCAATAGATGGGCAGACCTATACACTTTCAAGGAACAACTTCTTTATGAAAATTTGGAAGTGCTGTGGGACACTGTTTTCTCTTGGAACCGTGGAGAGAAGAGATGCTTTTGGCATACTCTCCTTGTACCTGTCTTTTTTTCCTTTTACAGAAGAAAGTGGAGATACCAACATGAGTGATAAAGTGGAATGTTTTGATATAAGAGCTGAAACAAAATTTTGGGACGAGATTAAAAAAGGCCTGGTTGATAAGGAGAGCTTAGTGAGGAAGCAATCGTTACACATCTTAAAGATAGCACTGCGCATAAATGGAGCAGCCCAATCCTCTGGTGTTTTAGAGGATATATCAGCTGAAAATAATTCAATGCCTCACAGCATGACAAAGAGGGAGCAGTGGGCTGATAAGGAAGCTAAATCTTTGGGTGTGGGGAAAATATGTAGCCAAGTTGAGTCTTCTCCGGACAGCCAGAACAAGTGGGAGGCATTTGTATTGCTGTATGAAATGCTTGAAGAGTATGGAACTCACCTCGTTGAAGCAGCTTGGAATTACCAGGTATCCTTGTTACTTCGAAACTCTAGCTCAGATGCTAATAATAAAACCTCTATCAGTGGAGCAGTTCATCAAAACCAGATCGAAAGTCCAGGAGAAATCTTTAACTGGTTAGCAATTTTGTGGGAACGGGGTTTTAATCATGACAATCCTCAAGTTAGGTGCTTGATCATGCAGTCATTTTTAGGGATTGATTGGAAGAATTACGGCACACATGCAAGATTGGTGCCTGGATATTTTGTTCTTGGTTCATTTATACAAGGGCTGAATGACCCTGTGCATCACAAAGAATTTGGTGTAAAAGGAATATACTCCTCAATGACAATAGAAGGTGCAGCTAACTTTCTAAGCAAATATACAAGTTATCTGAATGTGAGGAAGTGCATTATGTTTTTGAGTGACTTAGCATCTGCTGCTAAGCGGCAACCTCTTGGTAGAGTTGGATTGATGTGCCTTGCTGAATGCATTTCTTCTGCTGCTTGTCAAGTCGGAAAGAATAATAGTGAAATTGATGCACAGTGCTTTGAAGATGGAGTTCATGACATGATCAAAGTGGGAAAGTCTCCTCACAATGACAAAATTGTTCTGCTTGATGCCTTGAGATTTATTATTGAGAGCAGCAAACAACATTTCAATTCTAATTATCGTCTTCGAGTTTGTGAGAAAGTCCTTGACTCTGCTGCTTCAGTGGTGTGTACATGTGATCTGCCGCTCGATGTACTTTTGCACTTCATTTCAACATTTCCATGGGAGTTCACTGATCATGGCGGTCCCTTAAGAGAGAAAATACGACAATGGCTTATGGGGTGTGGTAAGCGTTGTAGTGTCAACTGCTGCAGTACTGAAATGAAGCTCCTGAAGAGTCTTTATGACTTTCCAAATAGGTTTATCAGCCATTGCAATGATGATGCTTCAACCACTTTTGACGATGAAGATTTGGATGCATGGGAATTTGAAGCGAAGCGGTGGGCAAGAGTGCTCTTTATTGCATGCAAGGAGGAATGTCAATTGTTACCCATACTGATGTTTATTCAAAATCACTGTAGTGATGTCTGCAAACAAAACAATAAGATGGACCTATTTCCTGTGAAGTTTTTCATCCTCATCTTGAGTCTAGTTCTGGAGCTTCAGATGATGCAAGAAAGAGTTGCCAAACATGGTTCTAAGCTTAGAACCAAATCAGAGTTTTTTGTGCCAGAATCAATGAATGAATTTAGTCATGCAGATGTTCCTAATTTATATCAAAAGTTCACCGACATTTTTCTTCTCATATTGGAGGAGTTGATCTCTTTTGCAAACTTGTCTTGTTCCATATTCTCAAATAACAATACCACTACGAGAGATGCAACCCTTCCTGGTTCTGTAACCGGAAAACTCGGAGGACCTAGTCAACGCCGGTTGTCATCTTCTACCACTACTGCTGTATTGCAAGCAATTACGTCCATGAAAGCTGTTGCAACTATCTCCTCATTCTGTGCACGAGTTAAAACTGATGTTTCACTTGATCTTCCTATTAATTTTTTGTGGGATTTCTACTGGAAGACTATTTCGTCTCCAGCTTGTGATTCAGAGACTGGAGCAGAAATACGTCTTGCAGCATATGAAGCATTAGCTCCTACTCTCACAGCACTAGTGTCTGTGTGTTCTCCTCAAACTTTGGATATACTTAAGAAAAATGACAGCTCATTACTATCAGATGGCAAACCTCTGTTGGATTCTTTGGTGCTTTCTTTTCTTCAGAACATAAATAAGCTCCTTACAGTTGGAGTATTTGTACGATCTCGACGTGCAATTTTAATGAATTGGAAGTGGATTTGCCTCGAATCTCTACTGTCAATTCCTTGCTATGCTCTTAGGAATGGGCTTCTTCTAGAGGCAAATAATTTTGTCTTCTCAGGTGCTGCTCTCCGATGTATTTTTTCTGATATTCTTGAGAGCCTTGAGAATGCTGGGGAAGGTTCTGTTTTACCGATGCTCAGATCTGTCCGATTAGTTTTGGACTTATTTGCCCAAGGAAAGTCTGGTTCACTTGTCTGCTTATGTGATGGGGTGGATTCCCAGATGCTGTGGCATTTGGTTCATTCTTCTTGGATATTGCATGTCAGTTGCAACAAGCGGAAGGTTGCACCTATTGCTGCACTTTTGTCTTCCGTTCTCCATTCATCTCTATTTAGTGATGAGAGCATGCATATAATTGATGGTGCACCTGGGCCTCTGAAGTGGTTTGTTGAGAAAATTCTAGAGGAAGGCACGAAAAGTCCCCGTACAATTCGTCTTGCTGCATTACACTTAACAGGGCTGTGGCTATCATATCCTAGCATTATAAAGTACTATGTGAAGGAGCTGAAGCTGCTATCTCTACATGGTTCCGTTGCATTCGATGAGGACTTCGAAGGTGAATTAGCTGATAATCGTGATACCAGGGCTGAAGTTTCTTTGTTGGCAAAAGGCCCTGATCCTGAGCTGACCAAAGCATTTATTAATACAGAGTTGTATGCACGGGCCTCTGTTGCTGTTTTGTTTTACAAGCTAGCAGACATGGCTGTCATGGTGGGATCAACTAATGAAAATGCAGATTGCCATGCAGCTTTGGAATCTGGAAAAATGTTTTTGCTTGAGCTTCTTGATTCCGTGGTAAACGACAAGGATCTTGCAAAAGAGTTGTATAAGAAGTATAGTGCAATTCATAGAAGAAAGGTACGGGCATGGCAAATGATATGTATTTTGTCGCATTTCGTCTGTCAAGATATAGTTTCAGAAGTTGCACGTTGCTTGCACACATCCCTCTATAGGAACAACTTACCTGCAGTTCGTCAATATTTGGAAACATTTGCAATTAATATTTACTTAAAGTTTCCATCACTGGTTGGGGAGCAACTAGTTCCTATATTAAGAGATTATGATATGAGGCCTCAGGCTCTCTCTTCTTACGTATTTATAGCAGCTAACGTCATCCTCCATGCATCTCAATCTGTTCAACACCGACATCTGAATGAATTACTTCCTCCTATAGTTCCACTATTAACCTCACATCACCACAGTCTACGTGGTTTCACCCAGTTGCTGGTTTACCAAGTTCTTTGCAAATTGTTCCCTCCATTGGATTCTAAAGCCTCTGAAACTATGACTTTAGAGATGAGGTGTTTTGAGGATCTCAAATCCTACCTCGCAAAGAATTCTGATTGTACGCGCTTACGACAATCAATGGAAGGATATCTTGATGCCTACAGTCCCCATAGCTCGGTCACTCCTGCGGGCGTTTTCATCAACCGGGTTGAGGATGTTGAGTTTGAATGTGTACCAATATCCCTTATGGAGCAAGTGCTCAACTTTTTGAATGATGTGAGGGAAGACCTTCGAAATTCAATGGCAAAGGATGTTGTGACTATTAAGAATGAGAGTTTGAGATCTGATGGAGATCAAGACCGCATGGCGATACTGTCTAATGCTAATGAAAGAAAATTGAGTACCCAGCTGCCTAAAGATATCTCACTAGATTTTCAGAAGAAGATTACTCTGTCTAAACACGAGAAGCAAGACATAAACGTTAATTCTTTCTTGGGCAACCAAGAAACTTACAAACAACTTCAGGAAATAGAAAAGGACGATAAGCTTCTTGCTCAGGTGCTGCAGTCTAGAACTCTAGGAATGGAAAGAGAGAGATCAAGCAGACAACATCTTATCCTTGTAGCATCTCTGCTTGATCGGATACCTAACCTAGCTGGCCTTGCAAGGACTTGTGAGGTATTTAAAGCATCAAGTTTGGCCATTGCGGATACGAACGTGTTACATGACAAACAGTTCCAACTCATCAGTGTGACTGCAGAGAAGTGGGTTCCTATCATCGAAGTCCCAGTGAATAGTTTGAAGGTGTTCCTTCAGAAAAAGAAACGAGAAGGCTTCTCAATTTTAGGTCTAGAGCAGACTGCAAATAGTATACCTCTTGATCAATATATATTTCCAAAAGACACAGTACTGGTTCTTGGGCGAGAGAAGGAAGGTATACCAGTTGATATAATACATATTCTGGATGCTTGCCTTGAGATTCCACAGTTGGGAGTTGTTAGATCTCTCAATGTTCATGTCAGTGGTGCCATTGCTCTGTGGGAGTATACTAGACAGCAGAGATCCCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000451 GO:0000453 GO:0001510 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006355 GO:0006357 GO:0006364 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008173 GO:0008175 GO:0008649 GO:0008757 GO:0009451 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016072 GO:0016423 GO:0016435 GO:0016740 GO:0016741 GO:0019219 GO:0019222 GO:0022613 GO:0030488 GO:0031167 GO:0031323 GO:0031326 GO:0032259 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070039 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0140098 GO:0140101 GO:0140102 GO:1901360 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SpoU_methylase

Protein Analysis

1841

Amino Acids

206.17

Weight (kDa)

6.17

Isoelectric Point (pI)

45.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SpoU_methylase	PF00588	1689 - 1831	4.9e-25	SpoU rRNA Methylase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008435)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G17610 AT4G17610 AT4G17610
fragaria_vesca	FvH4_5g20370 FvH4_5g20370 FvH4_5g20370 FvH4_5g20370 FvH4_5g20370
malus_domestica	MD04G1055600.v1.1
prunus_persica	Prupe.5G055400_v2.0.a1 Prupe.5G055400_v2.0.a1
pyrus_communis	pycom04g04890
rosa_chinensis	RchiOBHm_Chr7g0205631
rosa_laevigata	RLG00000003369
rosa_multiflora	Rmu_ssc0000482.1_g000025
rosa_roxburghii	Rroxscaffold_3G00252130
rosa_rugosa	Rorug07G0092300
rosa_samantha	Rh7AG223100 Rh7AG230100 Rh7BG218100 Rh7CG236300 Rh7DG230000
rosa_wichuraiana	Rw7G019240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3710
AasI	GACNNNNNNGTC	2 cut(s)	2935, 3929
Acc16I	TGCGCA	1 cut(s)	1139
Acc36I	ACCTGC	1 cut(s)	4198
AccB1I	GGYRCC	3 cut(s)	433, 1612, 5477
AccB7I	CCANNNNNTGG	3 cut(s)	25, 202, 4450
AccBSI	CCGCTC	1 cut(s)	2098
AccI	GTMKAC	5 cut(s)	5, 4429, 5323, 5389, 5498
AccII	CGCG	1 cut(s)	4576
AccIII	TCCGGA	1 cut(s)	1294
AclI	AACGTT	1 cut(s)	4932
AclWI	GGATC	7 cut(s)	523, 583, 3836, 3949, 4041, 4545, 5508
AcoI	YGGCCR	2 cut(s)	87, 5151
AfiI	CCNNNNNNNGG	9 cut(s)	25, 76, 202, 4176, 4450, 4486, 4627, 4663, 5441
AflII	CTTAAG	2 cut(s)	2161, 3053
AflIII	ACRYGT	3 cut(s)	339, 2084, 5169
AgeI	ACCGGT	1 cut(s)	765
AhdI	GACNNNNNGTC	1 cut(s)	717
AhlI	ACTAGT	2 cut(s)	3016, 4264
AjiI	CACGTC	1 cut(s)	3173
AjnI	CCWGG	9 cut(s)	407, 571, 1077, 1386, 1477, 1615, 2740, 3592, 3806
AleI	CACNNNNGTG	1 cut(s)	2430
AloI	GAACNNNNNNTCC	2 cut(s)	1611, 1643
Alw21I	GWGCWC	4 cut(s)	2367, 2866, 3592, 4713
Alw26I	GTCTC	5 cut(s)	129, 392, 1953, 2943, 2951
Alw44I	GTGCAC	2 cut(s)	2862, 3588
AlwI	GGATC	7 cut(s)	523, 583, 3836, 3949, 4041, 4545, 5508
AlwNI	CAGNNNCTG	5 cut(s)	2960, 3287, 3451, 4450, 5014
Aor13HI	TCCGGA	1 cut(s)	1294
AoxI	GGCC	9 cut(s)	87, 108, 1075, 3596, 3835, 3888, 4295, 5110, 5151
ApaLI	GTGCAC	2 cut(s)	2862, 3588
AseI	ATTAAT	4 cut(s)	384, 2901, 3867, 4224
AsiGI	ACCGGT	1 cut(s)	765
Asp700I	GAANNNNTTC	5 cut(s)	875, 2480, 4386, 4740, 5258
AspLEI	GCGC	2 cut(s)	1140, 4578
AspS9I	GGNCC	7 cut(s)	768, 2156, 2461, 2768, 3596, 3836, 3888
AsuC2I	CCSGG	2 cut(s)	527, 4658
AsuHPI	GGTGA	9 cut(s)	154, 245, 570, 701, 1352, 2629, 3794, 4412, 4432
AsuII	TTCGAA	3 cut(s)	1405, 3777, 4744
AsuNHI	GCTAGC	1 cut(s)	3916
AvaII	GGWCC	4 cut(s)	768, 2156, 2461, 2768
AxyI	CCTNAGG	1 cut(s)	4299
BaeGI	GKGCMC	3 cut(s)	247, 2866, 3592
BaeI	ACNNNNGTAYC	2 cut(s)	5154, 5187
BalI	TGGCCA	1 cut(s)	5153
BanI	GGYRCC	3 cut(s)	433, 1612, 5477
BauI	CACGAG	2 cut(s)	2865, 4913
BbsI	GAAGAC	6 cut(s)	1281, 2027, 2933, 3268, 3525, 4743
Bbv12I	GWGCWC	4 cut(s)	2367, 2866, 3592, 4713
BccI	CCATC	9 cut(s)	325, 839, 1910, 2452, 3077, 3425, 3578, 4252, 4793
BceAI	ACGGC	2 cut(s)	74, 1609
BciT130I	CCWGG	9 cut(s)	409, 573, 1079, 1388, 1479, 1617, 2742, 3594, 3808
BciVI	GTATCC	3 cut(s)	1402, 5088, 5155
BclI	TGATCA	4 cut(s)	1554, 1932, 2146, 5332
BcnI	CCSGG	2 cut(s)	527, 4658
BcoDI	GTCTC	5 cut(s)	129, 392, 1953, 2943, 2951
BcuI	ACTAGT	2 cut(s)	3016, 4264
BfmI	CTRYAG	8 cut(s)	2221, 2428, 4174, 4191, 4395, 4612, 5015, 5208
BfrI	CTTAAG	2 cut(s)	2161, 3053
BfuAI	ACCTGC	1 cut(s)	4198
BfuI	GTATCC	3 cut(s)	1402, 5088, 5155
BglII	AGATCT	3 cut(s)	3365, 4793, 5453
BlpI	GCTNAGC	1 cut(s)	1803
BmcAI	AGTACT	3 cut(s)	2227, 3716, 5361
Bme18I	GGWCC	4 cut(s)	768, 2156, 2461, 2768
BmeRI	GACNNNNNGTC	1 cut(s)	717
BmgBI	CACGTC	1 cut(s)	3173
BmgT120I	GGNCC	7 cut(s)	768, 2156, 2461, 2768, 3596, 3836, 3888
BmiI	GGNNCC	7 cut(s)	435, 925, 1614, 2158, 3754, 5223, 5479
BmrI	ACTGGG	3 cut(s)	4438, 5234, 5512
BmtI	GCTAGC	1 cut(s)	3920
BmuI	ACTGGG	3 cut(s)	4438, 5234, 5512
BpiI	GAAGAC	6 cut(s)	1281, 2027, 2933, 3268, 3525, 4743
BplI	GAGNNNNNCTC	2 cut(s)	2657, 2689
BpmI	CTGGAG	3 cut(s)	2528, 2925, 2982
Bpu10I	CCTNAGC	2 cut(s)	3846, 5007
Bpu1102I	GCTNAGC	1 cut(s)	1803
Bpu14I	TTCGAA	3 cut(s)	1405, 3777, 4744
BpuEI	CTTGAG	7 cut(s)	1524, 2005, 2515, 3338, 3347, 4022, 5447
BpuMI	CCSGG	2 cut(s)	527, 4658
BsaAI	YACGTR	2 cut(s)	4318, 4433
BsaBI	GATNNNNATC	1 cut(s)	4803
BsaJI	CCNNGG	7 cut(s)	408, 927, 2132, 3397, 3593, 3807, 4017
BsaWI	WCCGGW	3 cut(s)	765, 1294, 2753
BsaXI	ACNNNNNCTCC	2 cut(s)	3144, 3174
Bsc4I	CCNNNNNNNGG	9 cut(s)	25, 76, 202, 4176, 4450, 4486, 4627, 4663, 5441
Bse118I	RCCGGY	3 cut(s)	116, 765, 2781
Bse21I	CCTNAGG	1 cut(s)	4299
Bse3DI	GCAATG	4 cut(s)	2281, 2293, 5154, 5481
Bse8I	GATNNNNATC	1 cut(s)	4803
BseAI	TCCGGA	1 cut(s)	1294
BseBI	CCWGG	9 cut(s)	409, 573, 1079, 1388, 1479, 1617, 2742, 3594, 3808
BseDI	CCNNGG	7 cut(s)	408, 927, 2132, 3397, 3593, 3807, 4017
BseGI	GGATG	9 cut(s)	558, 603, 2332, 2484, 4166, 4338, 4672, 4768, 5422
BseJI	GATNNNNATC	1 cut(s)	4803
BseLI	CCNNNNNNNGG	9 cut(s)	25, 76, 202, 4176, 4450, 4486, 4627, 4663, 5441
BseMI	GCAATG	4 cut(s)	2281, 2293, 5154, 5481
BseRI	GAGGAG	6 cut(s)	646, 1690, 1941, 2678, 2842, 3024
BseSI	GKGCMC	3 cut(s)	247, 2866, 3592
BseX3I	CGGCCG	1 cut(s)	87
BseYI	CCCAGC	2 cut(s)	3335, 4857
BsgI	GTGCAG	2 cut(s)	1740, 3507
Bsh1236I	CGCG	1 cut(s)	4576
Bsh1285I	CGRYCG	2 cut(s)	90, 131
BshFI	GGCC	9 cut(s)	89, 110, 1077, 3598, 3837, 3890, 4297, 5112, 5153
BshNI	GGYRCC	3 cut(s)	433, 1612, 5477
BshTI	ACCGGT	1 cut(s)	765
BsiEI	CGRYCG	2 cut(s)	90, 131
BsiHKAI	GWGCWC	4 cut(s)	2367, 2866, 3592, 4713
BsiSI	CCGG	9 cut(s)	74, 86, 117, 526, 766, 1295, 2754, 2782, 4657
BslFI	GGGAC	7 cut(s)	53, 919, 1073, 2142, 3630, 4602, 5222
BslI	CCNNNNNNNGG	9 cut(s)	25, 76, 202, 4176, 4450, 4486, 4627, 4663, 5441
BsmAI	GTCTC	5 cut(s)	129, 392, 1953, 2943, 2951
BsmBI	CGTCTC	1 cut(s)	2943
BsmFI	GGGAC	7 cut(s)	53, 919, 1073, 2142, 3630, 4602, 5222
BsmI	GAATGC	3 cut(s)	1853, 3337, 3761
BsnI	GGCC	9 cut(s)	89, 110, 1077, 3598, 3837, 3890, 4297, 5112, 5153
Bsp119I	TTCGAA	3 cut(s)	1405, 3777, 4744
Bsp1286I	GDGCHC	5 cut(s)	247, 2367, 2866, 3592, 4713
Bsp13I	TCCGGA	1 cut(s)	1294
Bsp1407I	TGTACA	1 cut(s)	2081
Bsp1720I	GCTNAGC	1 cut(s)	1803
Bsp19I	CCATGG	1 cut(s)	2132
BspANI	GGCC	9 cut(s)	89, 110, 1077, 3598, 3837, 3890, 4297, 5112, 5153
BspEI	TCCGGA	1 cut(s)	1294
BspFNI	CGCG	1 cut(s)	4576
BspHI	TCATGA	3 cut(s)	183, 1528, 1924
BspLI	GGNNCC	7 cut(s)	435, 925, 1614, 2158, 3754, 5223, 5479
BspMAI	CTGCAG	4 cut(s)	2225, 4195, 5019, 5212
BspMI	ACCTGC	1 cut(s)	4198
BspOI	GCTAGC	1 cut(s)	3920
BspPI	GGATC	7 cut(s)	523, 583, 3836, 3949, 4041, 4545, 5508
BspT104I	TTCGAA	3 cut(s)	1405, 3777, 4744
BspT107I	GGYRCC	3 cut(s)	433, 1612, 5477
BspTI	CTTAAG	2 cut(s)	2161, 3053
BsrBI	CCGCTC	1 cut(s)	2098
BsrDI	GCAATG	4 cut(s)	2281, 2293, 5154, 5481
BsrFI	RCCGGY	3 cut(s)	116, 765, 2781
BsrGI	TGTACA	1 cut(s)	2081
BssAI	RCCGGY	3 cut(s)	116, 765, 2781
BssECI	CCNNGG	7 cut(s)	408, 927, 2132, 3397, 3593, 3807, 4017
BssNAI	GTATAC	3 cut(s)	5324, 5390, 5499
BssSI	CACGAG	2 cut(s)	2865, 4913
BssT1I	CCWWGG	2 cut(s)	2132, 3397
Bst1107I	GTATAC	3 cut(s)	5324, 5390, 5499
Bst2BI	CACGAG	2 cut(s)	2865, 4913
Bst2UI	CCWGG	9 cut(s)	409, 573, 1079, 1388, 1479, 1617, 2742, 3594, 3808
Bst6I	CTCTTC	4 cut(s)	931, 1341, 2240, 4315
BstAFI	CTTAAG	2 cut(s)	2161, 3053
BstAPI	GCANNNNNTGC	2 cut(s)	1799, 3518
BstAUI	TGTACA	1 cut(s)	2081
BstBAI	YACGTR	2 cut(s)	4318, 4433
BstBI	TTCGAA	3 cut(s)	1405, 3777, 4744
BstC8I	GCNNGC	8 cut(s)	2377, 2817, 3572, 3918, 4160, 4642, 5110, 5422
BstDSI	CCRYGG	3 cut(s)	927, 2132, 4017
BstF5I	GGATG	9 cut(s)	558, 603, 2332, 2484, 4166, 4338, 4672, 4768, 5422
BstFNI	CGCG	1 cut(s)	4576
BstHHI	GCGC	2 cut(s)	1140, 4578
BstMAI	GTCTC	5 cut(s)	129, 392, 1953, 2943, 2951
BstMCI	CGRYCG	2 cut(s)	90, 131
BstMWI	GCNNNNNNNGC	8 cut(s)	86, 1799, 2990, 3518, 3736, 3896, 3926, 5114
BstNI	CCWGG	9 cut(s)	409, 573, 1079, 1388, 1479, 1617, 2742, 3594, 3808
BstNSI	RCATGY	7 cut(s)	343, 358, 1604, 2088, 2379, 3488, 3574
BstSFI	CTRYAG	8 cut(s)	2221, 2428, 4174, 4191, 4395, 4612, 5015, 5208
BstSLI	GKGCMC	3 cut(s)	247, 2866, 3592
BstSNI	TACGTA	1 cut(s)	4318
BstUI	CGCG	1 cut(s)	4576
BstV2I	GAAGAC	6 cut(s)	1281, 2027, 2933, 3268, 3525, 4743
BstX2I	RGATCY	7 cut(s)	515, 3365, 4033, 4537, 4793, 5453, 5513
BstXI	CCANNNNNNTGG	1 cut(s)	1163
BstYI	RGATCY	7 cut(s)	515, 3365, 4033, 4537, 4793, 5453, 5513
BstZ17I	GTATAC	3 cut(s)	5324, 5390, 5499
BstZI	CGGCCG	1 cut(s)	87
Bsu36I	CCTNAGG	1 cut(s)	4299
BsuI	GTATCC	3 cut(s)	1402, 5088, 5155
BsuRI	GGCC	9 cut(s)	89, 110, 1077, 3598, 3837, 3890, 4297, 5112, 5153
BtgI	CCRYGG	3 cut(s)	927, 2132, 4017
BtrI	CACGTC	1 cut(s)	3173
BtsCI	GGATG	9 cut(s)	558, 603, 2332, 2484, 4166, 4338, 4672, 4768, 5422
BtsI	GCAGTG	2 cut(s)	1133, 1235
BveI	ACCTGC	1 cut(s)	4198
Cac8I	GCNNGC	8 cut(s)	2377, 2817, 3572, 3918, 4160, 4642, 5110, 5422
CaiI	CAGNNNCTG	5 cut(s)	2960, 3287, 3451, 4450, 5014
CciI	TCATGA	3 cut(s)	183, 1528, 1924
CfoI	GCGC	2 cut(s)	1140, 4578
Cfr10I	RCCGGY	3 cut(s)	116, 765, 2781
Cfr13I	GGNCC	7 cut(s)	768, 2156, 2461, 2768, 3596, 3836, 3888
CsiI	ACCWGGT	1 cut(s)	1386
CspAI	ACCGGT	1 cut(s)	765
DraI	TTTAAA	1 cut(s)	5137
DrdI	GACNNNNNNGTC	2 cut(s)	2935, 3929
DriI	GACNNNNNGTC	1 cut(s)	717
DseDI	GACNNNNNNGTC	2 cut(s)	2935, 3929
EaeI	YGGCCR	2 cut(s)	87, 5151
EagI	CGGCCG	1 cut(s)	87
Eam1104I	CTCTTC	4 cut(s)	931, 1341, 2240, 4315
Eam1105I	GACNNNNNGTC	1 cut(s)	717
EarI	CTCTTC	4 cut(s)	931, 1341, 2240, 4315
EclXI	CGGCCG	1 cut(s)	87
Eco105I	TACGTA	1 cut(s)	4318
Eco130I	CCWWGG	2 cut(s)	2132, 3397
Eco147I	AGGCCT	2 cut(s)	1077, 4297
Eco32I	GATATC	2 cut(s)	4601, 4873
Eco47I	GGWCC	4 cut(s)	768, 2156, 2461, 2768
Eco52I	CGGCCG	1 cut(s)	87
Eco81I	CCTNAGG	1 cut(s)	4299
EcoO109I	RGGNCCY	2 cut(s)	2768, 3836
EcoRI	GAATTC	1 cut(s)	4561
EcoRII	CCWGG	9 cut(s)	407, 571, 1077, 1386, 1477, 1615, 2740, 3592, 3806
EcoRV	GATATC	2 cut(s)	4601, 4873
EcoT14I	CCWWGG	2 cut(s)	2132, 3397
EcoT22I	ATGCAT	4 cut(s)	1853, 2332, 3576, 4351
ErhI	CCWWGG	2 cut(s)	2132, 3397
Esp3I	CGTCTC	1 cut(s)	2943
FalI	AAGNNNNNCTT	6 cut(s)	928, 960, 1079, 1111, 4587, 4619
FaqI	GGGAC	7 cut(s)	53, 919, 1073, 2142, 3630, 4602, 5222
FauI	CCCGC	1 cut(s)	4633
FauNDI	CATATG	2 cut(s)	537, 2986
FbaI	TGATCA	4 cut(s)	1554, 1932, 2146, 5332
FblI	GTMKAC	5 cut(s)	5, 4429, 5323, 5389, 5498
FokI	GGATG	9 cut(s)	545, 590, 2339, 2471, 4153, 4325, 4679, 4775, 5429
FspI	TGCGCA	1 cut(s)	1139
GlaI	GCGC	2 cut(s)	1139, 4577
GsaI	CCCAGC	2 cut(s)	3339, 4861
GsuI	CTGGAG	3 cut(s)	2528, 2925, 2982
HaeIII	GGCC	9 cut(s)	89, 110, 1077, 3598, 3837, 3890, 4297, 5112, 5153
HapII	CCGG	9 cut(s)	74, 86, 117, 526, 766, 1295, 2754, 2782, 4657
HhaI	GCGC	2 cut(s)	1140, 4578
Hin6I	GCGC	2 cut(s)	1138, 4576
HinP1I	GCGC	2 cut(s)	1138, 4576
HincII	GTYRAC	2 cut(s)	2215, 2777
HindII	GTYRAC	2 cut(s)	2215, 2777
HindIII	AAGCTT	2 cut(s)	2552, 4997
HpaII	CCGG	9 cut(s)	74, 86, 117, 526, 766, 1295, 2754, 2782, 4657
HphI	GGTGA	9 cut(s)	154, 245, 570, 701, 1352, 2629, 3794, 4412, 4432
Hpy99I	CGWCG	2 cut(s)	136, 3174
HpyCH4IV	ACGT	9 cut(s)	2825, 2974, 3172, 4153, 4317, 4335, 4432, 4932, 5169
HpyF10VI	GCNNNNNNNGC	8 cut(s)	86, 1799, 2990, 3518, 3736, 3896, 3926, 5114
HpySE526I	ACGT	9 cut(s)	2825, 2974, 3172, 4153, 4317, 4335, 4432, 4932, 5169
HspAI	GCGC	2 cut(s)	1138, 4576
Kpn2I	TCCGGA	1 cut(s)	1294
Ksp22I	TGATCA	4 cut(s)	1554, 1932, 2146, 5332
MabI	ACCWGGT	1 cut(s)	1386
MaeII	ACGT	9 cut(s)	2825, 2974, 3172, 4153, 4317, 4335, 4432, 4932, 5169
MbiI	CCGCTC	1 cut(s)	2098
MfeI	CAATTG	2 cut(s)	740, 2393
MflI	RGATCY	7 cut(s)	515, 3365, 4033, 4537, 4793, 5453, 5513
MhlI	GDGCHC	5 cut(s)	247, 2367, 2866, 3592, 4713
MlsI	TGGCCA	1 cut(s)	5153
MluNI	TGGCCA	1 cut(s)	5153
MlyI	GAGTC	5 cut(s)	200, 1295, 2054, 2257, 2506
MmeI	TCCRAC	6 cut(s)	183, 1807, 1855, 3078, 3130, 5215
Mox20I	TGGCCA	1 cut(s)	5153
Mph1103I	ATGCAT	4 cut(s)	1853, 2332, 3576, 4351
MroI	TCCGGA	1 cut(s)	1294
MroXI	GAANNNNTTC	5 cut(s)	875, 2480, 4386, 4740, 5258
MscI	TGGCCA	1 cut(s)	5153
MslI	CAYNNNNRTG	9 cut(s)	301, 2131, 2286, 2408, 2430, 2610, 4377, 5201, 5472
Msp20I	TGGCCA	1 cut(s)	5153
MspA1I	CMGCKG	2 cut(s)	1187, 4861
MspCI	CTTAAG	2 cut(s)	2161, 3053
MspI	CCGG	9 cut(s)	74, 86, 117, 526, 766, 1295, 2754, 2782, 4657
MunI	CAATTG	2 cut(s)	740, 2393
Mva1269I	GAATGC	3 cut(s)	1853, 3337, 3761
MvaI	CCWGG	9 cut(s)	409, 573, 1079, 1388, 1479, 1617, 2742, 3594, 3808
MvnI	CGCG	1 cut(s)	4576
MwoI	GCNNNNNNNGC	8 cut(s)	86, 1799, 2990, 3518, 3736, 3896, 3926, 5114
NciI	CCSGG	2 cut(s)	527, 4658
NcoI	CCATGG	1 cut(s)	2132
NdeI	CATATG	2 cut(s)	537, 2986
NheI	GCTAGC	1 cut(s)	3916
NlaIV	GGNNCC	7 cut(s)	435, 925, 1614, 2158, 3754, 5223, 5479
NmuCI	GTSAC	6 cut(s)	251, 391, 1784, 4630, 4768, 5203
NsbI	TGCGCA	1 cut(s)	1139
NsiI	ATGCAT	4 cut(s)	1853, 2332, 3576, 4351
NspI	RCATGY	7 cut(s)	343, 358, 1604, 2088, 2379, 3488, 3574
NspV	TTCGAA	3 cut(s)	1405, 3777, 4744
OliI	CACNNNNGTG	1 cut(s)	2430
PaeI	GCATGC	2 cut(s)	2379, 3574
PagI	TCATGA	3 cut(s)	183, 1528, 1924
PceI	AGGCCT	2 cut(s)	1077, 4297
PciI	ACATGT	2 cut(s)	339, 2084
PcsI	WCGNNNNNNNCGW	1 cut(s)	3169
PctI	GAATGC	3 cut(s)	1853, 3337, 3761
PdmI	GAANNNNTTC	5 cut(s)	875, 2480, 4386, 4740, 5258
PflFI	GACNNNGTC	1 cut(s)	2772
PflMI	CCANNNNNTGG	3 cut(s)	25, 202, 4450
PfoI	TCCNGGA	1 cut(s)	1477
PinAI	ACCGGT	1 cut(s)	765
PleI	GAGTC	5 cut(s)	200, 1294, 2054, 2256, 2505
PpsI	GAGTC	5 cut(s)	200, 1294, 2054, 2256, 2505
Ppu21I	YACGTR	2 cut(s)	4318, 4433
PpuMI	RGGWCCY	1 cut(s)	2768
PscI	ACATGT	2 cut(s)	339, 2084
PshBI	ATTAAT	4 cut(s)	384, 2901, 3867, 4224
PsiI	TTATAA	1 cut(s)	3710
Psp1406I	AACGTT	1 cut(s)	4932
Psp5II	RGGWCCY	1 cut(s)	2768
Psp6I	CCWGG	9 cut(s)	407, 571, 1077, 1386, 1477, 1615, 2740, 3592, 3806
PspFI	CCCAGC	2 cut(s)	3335, 4857
PspGI	CCWGG	9 cut(s)	407, 571, 1077, 1386, 1477, 1615, 2740, 3592, 3806
PspN4I	GGNNCC	7 cut(s)	435, 925, 1614, 2158, 3754, 5223, 5479
PspPI	GGNCC	7 cut(s)	768, 2156, 2461, 2768, 3596, 3836, 3888
PspPPI	RGGWCCY	1 cut(s)	2768
PstI	CTGCAG	4 cut(s)	2225, 4195, 5019, 5212
PstNI	CAGNNNCTG	5 cut(s)	2960, 3287, 3451, 4450, 5014
PsuI	RGATCY	7 cut(s)	515, 3365, 4033, 4537, 4793, 5453, 5513
PsyI	GACNNNGTC	1 cut(s)	2772
PvuII	CAGCTG	2 cut(s)	1187, 4861
RseI	CAYNNNNRTG	9 cut(s)	301, 2131, 2286, 2408, 2430, 2610, 4377, 5201, 5472
Sau96I	GGNCC	7 cut(s)	768, 2156, 2461, 2768, 3596, 3836, 3888
ScaI	AGTACT	3 cut(s)	2227, 3716, 5361
SchI	GAGTC	5 cut(s)	200, 1295, 2054, 2257, 2506
SduI	GDGCHC	5 cut(s)	247, 2367, 2866, 3592, 4713
SexAI	ACCWGGT	1 cut(s)	1386
SfcI	CTRYAG	8 cut(s)	2221, 2428, 4174, 4191, 4395, 4612, 5015, 5208
SfuI	TTCGAA	3 cut(s)	1405, 3777, 4744
SinI	GGWCC	4 cut(s)	768, 2156, 2461, 2768
SmiMI	CAYNNNNRTG	9 cut(s)	301, 2131, 2286, 2408, 2430, 2610, 4377, 5201, 5472
SmlI	CTYRAG	9 cut(s)	1539, 1984, 2161, 2494, 3053, 3317, 3326, 4001, 5426
SmoI	CTYRAG	9 cut(s)	1539, 1984, 2161, 2494, 3053, 3317, 3326, 4001, 5426
SnaBI	TACGTA	1 cut(s)	4318
SpeI	ACTAGT	2 cut(s)	3016, 4264
SphI	GCATGC	2 cut(s)	2379, 3574
SseBI	AGGCCT	2 cut(s)	1077, 4297
SspI	AATATT	2 cut(s)	4205, 4228
StuI	AGGCCT	2 cut(s)	1077, 4297
StyI	CCWWGG	2 cut(s)	2132, 3397
TaiI	ACGT	9 cut(s)	2828, 2977, 3175, 4156, 4320, 4338, 4435, 4935, 5172
TaqII	GACCGA	2 cut(s)	117, 4618
TatI	WGTACW	6 cut(s)	548, 2081, 2104, 2225, 3714, 5359
TauI	GCSGC	4 cut(s)	80, 351, 1810, 2098
TseFI	GTSAC	6 cut(s)	251, 391, 1784, 4630, 4768, 5203
Tsp45I	GTSAC	6 cut(s)	251, 391, 1784, 4630, 4768, 5203
TspGWI	ACGGA	3 cut(s)	3526, 3745, 4006
Tth111I	GACNNNGTC	1 cut(s)	2772
Van91I	CCANNNNNTGG	3 cut(s)	25, 202, 4450
Vha464I	CTTAAG	2 cut(s)	2161, 3053
VneI	GTGCAC	2 cut(s)	2862, 3588
VpaK11BI	GGWCC	4 cut(s)	768, 2156, 2461, 2768
VspI	ATTAAT	4 cut(s)	384, 2901, 3867, 4224
XbaI	TCTAGA	4 cut(s)	3256, 3625, 5021, 5302
XceI	RCATGY	7 cut(s)	343, 358, 1604, 2088, 2379, 3488, 3574
XcmI	CCANNNNNNNNNTGG	2 cut(s)	3404, 4430
XmiI	GTMKAC	5 cut(s)	5, 4429, 5323, 5389, 5498
XmnI	GAANNNNTTC	5 cut(s)	875, 2480, 4386, 4740, 5258
ZrmI	AGTACT	3 cut(s)	2227, 3716, 5361
Zsp2I	ATGCAT	4 cut(s)	1853, 2332, 3576, 4351

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.