AT4G25030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007057
Physical Location & Seq
Forward (+)
12864452 .. 12866827
2376 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25030.1

Sequence Viewer

Length: 1035 bp
ATGGATAATTGTACTGGAGAAAATCCCATGGCTTCTCAGTTGAACATTCTCCGATGCCCGTTCTTGAGGAACATCAATGAGCCCACTAACCTCTCTTTCTCTTCATCCTTGCCTTTTCCCATCCCTGCAAGAGCTGGGAAAGGACCTATATTTGAAGATGGTCCCAATTTTGATACGGCGTTTAGGCTTTTCCATGGCCAAGATGGAGTTGTCCCTCTCTCTGACACCGCTCGAACTGAAGCCCAGAAGCCTGTGCCTGTATTCCATCCACTTGCTGCTAAGGCGGCCACTATTAGTCTCTCATCCTTTGGATCTGGAGGGCCTTTTGGATTTGATGCATTTTCTGACATGTTTAAAAATCAAAAGAAGAAGTCAGATTCATCCAAAAATAAGGGAGGAAACCACGAAGCCATGGGCGACGAATGGCTTAAAACAGGAAACTGCCCAATAGCAAAATCATATCGAGCTGTAAGTGGTGTGGCACCACTTGTGGCAAAGATCCTGCAACCCCCTCCAGGCATGAAATTTAAATGCCCTCAAGCAATAGTCACAGCTCGAGCAGCGATATCAAAAACACCTTTCGCCAAGAACCTCCGTCCACAACCTTTACCAGCTAAAGTACTAGTGATCGGGATGCTGGGCATGGCGTTAAATGTGCCTTTAGGGGTCTGGAGAGAGCACACTGAAAAGTTTTCGGCATCTTGGTTTATAGCTCTTCACGCAGCGGTTCCATTCATAGGAATACTGAGAAAGTCAGTGTTGATGCCTAAAACAGCTATGGTATTCACCATAGCAGCATCAGTTTTGGGACAGGTGATTGGGTCAAGAGCAGAGAGGCGGAGACTCAAGTCTGTAGCTGAGAAGAAGTTGACATTGGAGGTACCAAATCCGTCTTCTGTTGAAGCAGATCAGATGCAATTTGCCGGAGTTTCATCAGATGGAAGATGCGGTGATAAAGTGGTGATGAAGTGGAATCCCATGATGCTTGATGTTGCAAGCCCTGTGTCTTCCGGAGCAGCTACTGTTGTCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

344

Amino Acids

36.84

Weight (kDa)

9.72

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 880
AccB1I GGYRCC 2 cut(s) 481, 880
AccBSI CCGCTC 1 cut(s) 230
AccIII TCCGGA 1 cut(s) 1010
AciI CCGC 5 cut(s) 228, 284, 725, 838, 948
AclWI GGATC 2 cut(s) 319, 493
AcoI YGGCCR 2 cut(s) 196, 285
AcsI RAATTY 1 cut(s) 524
AcuI CTGAAG 1 cut(s) 258
AfaI GTAC 3 cut(s) 13, 621, 882
AfiI CCNNNNNNNGG 2 cut(s) 515, 737
AflIII ACRYGT 1 cut(s) 348
AgsI TTSAA 3 cut(s) 43, 155, 902
AhlI ACTAGT 1 cut(s) 622
AjnI CCWGG 1 cut(s) 514
AjuI GAANNNNNNNTTGG 4 cut(s) 857, 877, 889, 909
AluBI AGCT 8 cut(s) 134, 467, 554, 614, 713, 776, 857, 1019
AluI AGCT 8 cut(s) 134, 467, 554, 614, 713, 776, 857, 1019
Alw21I GWGCWC 1 cut(s) 681
Alw26I GTCTC 2 cut(s) 302, 835
AlwI GGATC 2 cut(s) 319, 493
AlwNI CAGNNNCTG 1 cut(s) 1022
Ama87I CYCGRG 1 cut(s) 555
Aor13HI TCCGGA 1 cut(s) 1010
AoxI GGCC 3 cut(s) 196, 285, 320
ApeKI GCWGC 5 cut(s) 275, 560, 722, 794, 1016
ApoI RAATTY 1 cut(s) 524
Asp718I GGTACC 1 cut(s) 880
AspS9I GGNCC 3 cut(s) 143, 161, 320
AsuHPI GGTGA 4 cut(s) 778, 826, 962, 973
AvaI CYCGRG 1 cut(s) 555
AvaII GGWCC 2 cut(s) 143, 161
BalI TGGCCA 1 cut(s) 198
BanI GGYRCC 2 cut(s) 481, 880
BanII GRGCYC 1 cut(s) 84
BbsI GAAGAC 2 cut(s) 885, 999
Bbv12I GWGCWC 1 cut(s) 681
BbvI GCAGC 5 cut(s) 262, 572, 734, 806, 1028
BccI CCATC 5 cut(s) 128, 152, 197, 273, 932
BceAI ACGGC 1 cut(s) 192
BciT130I CCWGG 1 cut(s) 516
BcoDI GTCTC 2 cut(s) 302, 835
BcuI ACTAGT 1 cut(s) 622
BfaI CTAG 1 cut(s) 623
BfmI CTRYAG 1 cut(s) 852
BisI GCNGC 6 cut(s) 276, 285, 561, 723, 795, 1017
BlsI GCNGC 6 cut(s) 277, 286, 562, 724, 796, 1018
BmcAI AGTACT 1 cut(s) 621
Bme1390I CCNGG 1 cut(s) 516
Bme18I GGWCC 2 cut(s) 143, 161
BmeT110I CYCGRG 1 cut(s) 555
BmgT120I GGNCC 3 cut(s) 143, 161, 320
BmiI GGNNCC 4 cut(s) 163, 483, 729, 882
BmrFI CCNGG 1 cut(s) 516
BmsI GCATC 9 cut(s) 44, 325, 624, 707, 753, 806, 903, 935, 972
BoxI GACNNNNGTC 1 cut(s) 847
BpiI GAAGAC 2 cut(s) 885, 999
BpmI CTGGAG 4 cut(s) 36, 336, 498, 691
Bpu10I CCTNAGC 1 cut(s) 279
BpuEI CTTGAG 3 cut(s) 85, 522, 830
BsaJI CCNNGG 3 cut(s) 27, 193, 411
BsaWI WCCGGW 1 cut(s) 1010
Bsc4I CCNNNNNNNGG 2 cut(s) 515, 737
Bse1I ACTGG 1 cut(s) 19
BseAI TCCGGA 1 cut(s) 1010
BseBI CCWGG 1 cut(s) 516
BseDI CCNNGG 3 cut(s) 27, 193, 411
BseGI GGATG 6 cut(s) 104, 120, 265, 302, 380, 639
BseLI CCNNNNNNNGG 2 cut(s) 515, 737
BseMII CTCAG 3 cut(s) 50, 737, 849
BseNI ACTGG 1 cut(s) 19
BseXI GCAGC 5 cut(s) 262, 572, 734, 806, 1028
BseYI CCCAGC 2 cut(s) 134, 637
BshFI GGCC 3 cut(s) 198, 287, 322
BshNI GGYRCC 2 cut(s) 481, 880
BsiHKAI GWGCWC 1 cut(s) 681
BsiHKCI CYCGRG 1 cut(s) 555
BsiSI CCGG 2 cut(s) 924, 1011
BslFI GGGAC 3 cut(s) 147, 197, 822
BslI CCNNNNNNNGG 2 cut(s) 515, 737
BsmAI GTCTC 2 cut(s) 302, 835
BsmFI GGGAC 3 cut(s) 147, 197, 822
BsnI GGCC 3 cut(s) 198, 287, 322
BsoBI CYCGRG 1 cut(s) 555
Bsp1286I GDGCHC 2 cut(s) 84, 681
Bsp13I TCCGGA 1 cut(s) 1010
Bsp143I GATC 4 cut(s) 311, 498, 627, 907
Bsp19I CCATGG 3 cut(s) 27, 193, 411
BspACI CCGC 5 cut(s) 228, 284, 725, 838, 948
BspANI GGCC 3 cut(s) 198, 287, 322
BspCNI CTCAG 3 cut(s) 49, 738, 850
BspEI TCCGGA 1 cut(s) 1010
BspLI GGNNCC 4 cut(s) 163, 483, 729, 882
BspPI GGATC 2 cut(s) 319, 493
BspQI GCTCTTC 1 cut(s) 720
BspT107I GGYRCC 2 cut(s) 481, 880
BsrBI CCGCTC 1 cut(s) 230
BsrI ACTGG 1 cut(s) 19
BssECI CCNNGG 3 cut(s) 27, 193, 411
BssMI GATC 4 cut(s) 311, 498, 627, 907
BssT1I CCWWGG 3 cut(s) 27, 193, 411
Bst2UI CCWGG 1 cut(s) 516
Bst4CI ACNGT 1 cut(s) 1024
Bst6I CTCTTC 2 cut(s) 106, 720
BstC8I GCNNGC 1 cut(s) 997
BstDEI CTNAG 4 cut(s) 36, 279, 746, 858
BstDSI CCRYGG 3 cut(s) 27, 193, 411
BstF5I GGATG 6 cut(s) 104, 120, 265, 302, 380, 639
BstKTI GATC 4 cut(s) 314, 501, 630, 910
BstMAI GTCTC 2 cut(s) 302, 835
BstMBI GATC 4 cut(s) 311, 498, 627, 907
BstMWI GCNNNNNNNGC 4 cut(s) 281, 284, 560, 719
BstNI CCWGG 1 cut(s) 516
BstNSI RCATGY 1 cut(s) 352
BstPAI GACNNNNGTC 1 cut(s) 847
BstSCI CCNGG 1 cut(s) 514
BstSFI CTRYAG 1 cut(s) 852
BstV1I GCAGC 5 cut(s) 262, 572, 734, 806, 1028
BstV2I GAAGAC 2 cut(s) 885, 999
BstX2I RGATCY 2 cut(s) 311, 498
BstYI RGATCY 2 cut(s) 311, 498
BsuRI GGCC 3 cut(s) 198, 287, 322
BtgI CCRYGG 3 cut(s) 27, 193, 411
BtsCI GGATG 6 cut(s) 104, 120, 265, 302, 380, 639
BtsIMutI CAGTG 2 cut(s) 681, 762
Cac8I GCNNGC 1 cut(s) 997
CaiI CAGNNNCTG 1 cut(s) 1022
Cfr13I GGNCC 3 cut(s) 143, 161, 320
Csp6I GTAC 3 cut(s) 12, 620, 881
CviAII CATG 7 cut(s) 28, 194, 349, 412, 520, 643, 979
CviQI GTAC 3 cut(s) 12, 620, 881
DdeI CTNAG 4 cut(s) 36, 279, 746, 858
DpnI GATC 4 cut(s) 313, 500, 629, 909
DpnII GATC 4 cut(s) 311, 498, 627, 907
DraI TTTAAA 2 cut(s) 355, 529
EaeI YGGCCR 2 cut(s) 196, 285
Eam1104I CTCTTC 2 cut(s) 106, 720
EarI CTCTTC 2 cut(s) 106, 720
EciI GGCGGA 1 cut(s) 853
Eco130I CCWWGG 3 cut(s) 27, 193, 411
Eco24I GRGCYC 1 cut(s) 84
Eco32I GATATC 1 cut(s) 567
Eco47I GGWCC 2 cut(s) 143, 161
Eco57I CTGAAG 1 cut(s) 258
Eco88I CYCGRG 1 cut(s) 555
EcoO109I RGGNCCY 2 cut(s) 143, 320
EcoRII CCWGG 1 cut(s) 514
EcoRV GATATC 1 cut(s) 567
EcoT14I CCWWGG 3 cut(s) 27, 193, 411
EcoT22I ATGCAT 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 3 cut(s) 27, 193, 411
FaeI CATG 7 cut(s) 31, 197, 352, 415, 523, 646, 982
FaqI GGGAC 3 cut(s) 147, 197, 822
FatI CATG 7 cut(s) 27, 193, 348, 411, 519, 642, 978
Fnu4HI GCNGC 6 cut(s) 276, 285, 561, 723, 795, 1017
FokI GGATG 6 cut(s) 91, 107, 252, 289, 367, 646
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 6 cut(s) 276, 285, 561, 723, 795, 1017
FspBI CTAG 1 cut(s) 623
GluI GCNGC 6 cut(s) 276, 285, 561, 723, 795, 1017
GsaI CCCAGC 2 cut(s) 138, 641
GsuI CTGGAG 4 cut(s) 36, 336, 498, 691
HaeIII GGCC 3 cut(s) 198, 287, 322
HapII CCGG 2 cut(s) 924, 1011
Hin1II CATG 7 cut(s) 31, 197, 352, 415, 523, 646, 982
HincII GTYRAC 1 cut(s) 870
HindII GTYRAC 1 cut(s) 870
HinfI GANTC 3 cut(s) 377, 843, 973
HpaII CCGG 2 cut(s) 924, 1011
HphI GGTGA 4 cut(s) 778, 826, 962, 973
Hpy166II GTNNAC 2 cut(s) 599, 870
Hpy188I TCNGA 6 cut(s) 53, 223, 346, 376, 912, 937
Hpy188III TCNNGA 6 cut(s) 64, 315, 631, 670, 825, 1011
Hpy8I GTNNAC 2 cut(s) 599, 870
Hpy99I CGWCG 1 cut(s) 422
HpyCH4III ACNGT 1 cut(s) 1024
HpyCH4V TGCA 5 cut(s) 128, 338, 505, 916, 995
HpyF10VI GCNNNNNNNGC 4 cut(s) 281, 284, 560, 719
HpyF3I CTNAG 4 cut(s) 36, 279, 746, 858
Hsp92II CATG 7 cut(s) 31, 197, 352, 415, 523, 646, 982
Kpn2I TCCGGA 1 cut(s) 1010
KpnI GGTACC 1 cut(s) 884
Kzo9I GATC 4 cut(s) 311, 498, 627, 907
LguI GCTCTTC 1 cut(s) 720
LmnI GCTCC 1 cut(s) 1013
Lsp1109I GCAGC 5 cut(s) 262, 572, 734, 806, 1028
LweI GCATC 9 cut(s) 44, 325, 624, 707, 753, 806, 903, 935, 972
MaeI CTAG 1 cut(s) 623
MaeIII GTNAC 1 cut(s) 547
MalI GATC 4 cut(s) 313, 500, 629, 909
MbiI CCGCTC 1 cut(s) 230
MboI GATC 4 cut(s) 311, 498, 627, 907
MboII GAAGA 8 cut(s) 93, 167, 379, 707, 874, 885, 954, 999
MflI RGATCY 2 cut(s) 311, 498
MhlI GDGCHC 2 cut(s) 84, 681
MlsI TGGCCA 1 cut(s) 198
MluCI AATT 4 cut(s) 7, 166, 524, 917
MluNI TGGCCA 1 cut(s) 198
MlyI GAGTC 1 cut(s) 837
Mox20I TGGCCA 1 cut(s) 198
Mph1103I ATGCAT 1 cut(s) 340
MroI TCCGGA 1 cut(s) 1010
MscI TGGCCA 1 cut(s) 198
MseI TTAA 4 cut(s) 354, 429, 528, 650
Msp20I TGGCCA 1 cut(s) 198
MspA1I CMGCKG 1 cut(s) 725
MspI CCGG 2 cut(s) 924, 1011
MspR9I CCNGG 1 cut(s) 516
MvaI CCWGG 1 cut(s) 516
MwoI GCNNNNNNNGC 4 cut(s) 281, 284, 560, 719
NcoI CCATGG 3 cut(s) 27, 193, 411
NdeII GATC 4 cut(s) 311, 498, 627, 907
NlaIII CATG 7 cut(s) 31, 197, 352, 415, 523, 646, 982
NlaIV GGNNCC 4 cut(s) 163, 483, 729, 882
NmuCI GTSAC 1 cut(s) 547
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 352
PaeR7I CTCGAG 1 cut(s) 555
PciI ACATGT 1 cut(s) 348
PciSI GCTCTTC 1 cut(s) 720
PfeI GAWTC 2 cut(s) 377, 973
PkrI GCNGC 6 cut(s) 277, 286, 562, 724, 796, 1018
PleI GAGTC 1 cut(s) 837
PpsI GAGTC 1 cut(s) 837
PpuMI RGGWCCY 1 cut(s) 143
PscI ACATGT 1 cut(s) 348
PshAI GACNNNNGTC 1 cut(s) 847
Psp5II RGGWCCY 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 514
PspFI CCCAGC 2 cut(s) 134, 637
PspGI CCWGG 1 cut(s) 514
PspN4I GGNNCC 4 cut(s) 163, 483, 729, 882
PspPI GGNCC 3 cut(s) 143, 161, 320
PspPPI RGGWCCY 1 cut(s) 143
PspXI VCTCGAGB 1 cut(s) 555
PstNI CAGNNNCTG 1 cut(s) 1022
PsuI RGATCY 2 cut(s) 311, 498
RsaI GTAC 3 cut(s) 13, 621, 882
RsaNI GTAC 3 cut(s) 12, 620, 881
SapI GCTCTTC 1 cut(s) 720
SaqAI TTAA 4 cut(s) 354, 429, 528, 650
SatI GCNGC 6 cut(s) 276, 285, 561, 723, 795, 1017
Sau3AI GATC 4 cut(s) 311, 498, 627, 907
Sau96I GGNCC 3 cut(s) 143, 161, 320
ScaI AGTACT 1 cut(s) 621
SchI GAGTC 1 cut(s) 837
ScrFI CCNGG 1 cut(s) 516
SduI GDGCHC 2 cut(s) 84, 681
SfaNI GCATC 9 cut(s) 44, 325, 624, 707, 753, 806, 903, 935, 972
SfcI CTRYAG 1 cut(s) 852
Sfr274I CTCGAG 1 cut(s) 555
SinI GGWCC 2 cut(s) 143, 161
SlaI CTCGAG 1 cut(s) 555
SmiI ATTTAAAT 1 cut(s) 529
SmlI CTYRAG 4 cut(s) 64, 537, 555, 845
SmoI CTYRAG 4 cut(s) 64, 537, 555, 845
SpeI ACTAGT 1 cut(s) 622
Sse9I AATT 4 cut(s) 7, 166, 524, 917
SsiI CCGC 5 cut(s) 228, 284, 725, 838, 948
SspMI CTAG 1 cut(s) 623
StyD4I CCNGG 1 cut(s) 514
StyI CCWWGG 3 cut(s) 27, 193, 411
SwaI ATTTAAAT 1 cut(s) 529
TaaI ACNGT 1 cut(s) 1024
TaqI TCGA 3 cut(s) 232, 463, 556
TasI AATT 4 cut(s) 7, 166, 524, 917
TatI WGTACW 2 cut(s) 11, 619
TauI GCSGC 1 cut(s) 287
TfiI GAWTC 2 cut(s) 377, 973
Tru1I TTAA 4 cut(s) 354, 429, 528, 650
Tru9I TTAA 4 cut(s) 354, 429, 528, 650
TscAI CASTG 2 cut(s) 688, 762
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 5 cut(s) 275, 560, 722, 794, 1016
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 6 cut(s) 93, 369, 536, 724, 921, 980
TspGWI ACGGA 2 cut(s) 584, 879
TspRI CASTG 2 cut(s) 688, 762
VpaK11BI GGWCC 2 cut(s) 143, 161
XapI RAATTY 1 cut(s) 524
XceI RCATGY 1 cut(s) 352
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
XhoI CTCGAG 1 cut(s) 555
XspI CTAG 1 cut(s) 623
ZrmI AGTACT 1 cut(s) 621
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.