AT4G25950

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008981
Physical Location & Seq
Reverse (-)
13173443 .. 13174541
1099 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25950.1

Sequence Viewer

Length: 327 bp
ATGGATTCTTTAAGAGGCCAAGGTGGGATTCAGATGCTTTTAACTGCTGAACAAGAAGCCGGACGGATTGTTTCAGCTGCGAGGACCGCAAAACTAGCAAGAATGAAGCAAGCGAAAGATGAGGCTGAGAAGGAGATGGAAGAATACAGATCCCGATTAGAAGAAGAGTATCAAACTCAAGTTTCTGGGACGGATCAAGAAGCAGATGCAAAACGTTTAGATGATGAGACAGATGTCAGGATTACGAACCTTAAGGAATCTAGTTCAAAGGTCTCCAAGGATATTGTCAAGATGCTCATCAAGTATGTCACCACTACTGCAGCATGA

Protein Analysis

108

Amino Acids

12.12

Weight (kDa)

5.11

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-ATPase_G PF03179 7 - 106 1.6e-29 Vacuolar (H+)-ATPase G subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 87
AclI AACGTT 1 cut(s) 214
AclWI GGATC 2 cut(s) 144, 201
AflII CTTAAG 1 cut(s) 251
AgsI TTSAA 1 cut(s) 267
AjuI GAANNNNNNNTTGG 2 cut(s) 12, 44
AluBI AGCT 1 cut(s) 77
AluI AGCT 1 cut(s) 77
Alw26I GTCTC 2 cut(s) 221, 277
AlwI GGATC 2 cut(s) 144, 201
AoxI GGCC 1 cut(s) 16
ApeKI GCWGC 2 cut(s) 77, 320
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 301
AvaII GGWCC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 64
BccI CCATC 1 cut(s) 130
BcoDI GTCTC 2 cut(s) 221, 277
BfaI CTAG 2 cut(s) 95, 261
BfmI CTRYAG 1 cut(s) 318
BfrI CTTAAG 1 cut(s) 251
BisI GCNGC 2 cut(s) 78, 321
BlsI GCNGC 2 cut(s) 79, 322
Bme18I GGWCC 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 84
BmsI GCATC 3 cut(s) 24, 196, 282
BoxI GACNNNNGTC 1 cut(s) 233
BpuEI CTTGAG 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 296
BsaI GGTCTC 1 cut(s) 277
BsaJI CCNNGG 2 cut(s) 19, 276
Bse8I GATNNNNATC 1 cut(s) 296
BseDI CCNNGG 2 cut(s) 19, 276
BseJI GATNNNNATC 1 cut(s) 296
BseMII CTCAG 1 cut(s) 117
BseXI GCAGC 1 cut(s) 64
BshFI GGCC 1 cut(s) 18
BsiSI CCGG 1 cut(s) 60
BslFI GGGAC 1 cut(s) 202
BsmAI GTCTC 2 cut(s) 221, 277
BsmFI GGGAC 1 cut(s) 202
BsnI GGCC 1 cut(s) 18
Bso31I GGTCTC 1 cut(s) 277
Bsp143I GATC 2 cut(s) 149, 193
BspACI CCGC 1 cut(s) 87
BspANI GGCC 1 cut(s) 18
BspCNI CTCAG 1 cut(s) 118
BspMAI CTGCAG 1 cut(s) 322
BspPI GGATC 2 cut(s) 144, 201
BspTI CTTAAG 1 cut(s) 251
BspTNI GGTCTC 1 cut(s) 277
BssECI CCNNGG 2 cut(s) 19, 276
BssMI GATC 2 cut(s) 149, 193
BssT1I CCWWGG 2 cut(s) 19, 276
Bst6I CTCTTC 1 cut(s) 159
BstAFI CTTAAG 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 111
BstDEI CTNAG 1 cut(s) 126
BstKTI GATC 2 cut(s) 152, 196
BstMAI GTCTC 2 cut(s) 221, 277
BstMBI GATC 2 cut(s) 149, 193
BstMWI GCNNNNNNNGC 2 cut(s) 86, 95
BstPAI GACNNNNGTC 1 cut(s) 233
BstSFI CTRYAG 1 cut(s) 318
BstV1I GCAGC 1 cut(s) 64
BstX2I RGATCY 1 cut(s) 149
BstYI RGATCY 1 cut(s) 149
BsuRI GGCC 1 cut(s) 18
Cac8I GCNNGC 1 cut(s) 111
Cfr13I GGNCC 1 cut(s) 84
CviAII CATG 1 cut(s) 324
CviJI RGCY 4 cut(s) 18, 59, 77, 125
CviKI_1 RGCY 4 cut(s) 18, 59, 77, 125
DdeI CTNAG 1 cut(s) 126
DpnI GATC 2 cut(s) 151, 195
DpnII GATC 2 cut(s) 149, 193
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco130I CCWWGG 2 cut(s) 19, 276
Eco31I GGTCTC 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 84
EcoT14I CCWWGG 2 cut(s) 19, 276
ErhI CCWWGG 2 cut(s) 19, 276
FaeI CATG 1 cut(s) 327
FaiI YATR 2 cut(s) 306, 325
FaqI GGGAC 1 cut(s) 202
FatI CATG 1 cut(s) 323
Fnu4HI GCNGC 2 cut(s) 78, 321
Fsp4HI GCNGC 2 cut(s) 78, 321
FspBI CTAG 2 cut(s) 95, 261
GluI GCNGC 2 cut(s) 78, 321
HaeIII GGCC 1 cut(s) 18
HapII CCGG 1 cut(s) 60
Hin1II CATG 1 cut(s) 327
HinfI GANTC 3 cut(s) 5, 28, 257
HpaII CCGG 1 cut(s) 60
HphI GGTGA 1 cut(s) 301
Hpy188I TCNGA 1 cut(s) 33
Hpy188III TCNNGA 4 cut(s) 153, 197, 238, 289
HpyAV CCTTC 1 cut(s) 124
HpyCH4IV ACGT 1 cut(s) 214
HpyCH4V TGCA 2 cut(s) 209, 320
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 95
HpyF3I CTNAG 1 cut(s) 126
HpySE526I ACGT 1 cut(s) 214
Hsp92II CATG 1 cut(s) 327
Kzo9I GATC 2 cut(s) 149, 193
LpnPI CCDG 3 cut(s) 73, 171, 223
Lsp1109I GCAGC 1 cut(s) 64
LweI GCATC 3 cut(s) 24, 196, 282
MaeI CTAG 2 cut(s) 95, 261
MaeII ACGT 1 cut(s) 214
MaeIII GTNAC 1 cut(s) 307
MalI GATC 2 cut(s) 151, 195
MboI GATC 2 cut(s) 149, 193
MboII GAAGA 3 cut(s) 152, 173, 176
MflI RGATCY 1 cut(s) 149
MnlI CCTC 3 cut(s) 8, 75, 115
MseI TTAA 3 cut(s) 11, 41, 252
MspA1I CMGCKG 1 cut(s) 77
MspCI CTTAAG 1 cut(s) 251
MspI CCGG 1 cut(s) 60
MwoI GCNNNNNNNGC 2 cut(s) 86, 95
NdeII GATC 2 cut(s) 149, 193
NlaIII CATG 1 cut(s) 327
NmuCI GTSAC 1 cut(s) 307
PfeI GAWTC 3 cut(s) 5, 28, 257
PkrI GCNGC 2 cut(s) 79, 322
PshAI GACNNNNGTC 1 cut(s) 233
Psp1406I AACGTT 1 cut(s) 214
PspPI GGNCC 1 cut(s) 84
PstI CTGCAG 1 cut(s) 322
PsuI RGATCY 1 cut(s) 149
PvuII CAGCTG 1 cut(s) 77
SaqAI TTAA 3 cut(s) 11, 41, 252
SatI GCNGC 2 cut(s) 78, 321
Sau3AI GATC 2 cut(s) 149, 193
Sau96I GGNCC 1 cut(s) 84
SetI ASST 5 cut(s) 25, 79, 217, 252, 273
SfaNI GCATC 3 cut(s) 24, 196, 282
SfcI CTRYAG 1 cut(s) 318
SinI GGWCC 1 cut(s) 84
SmlI CTYRAG 2 cut(s) 177, 251
SmoI CTYRAG 2 cut(s) 177, 251
SsiI CCGC 1 cut(s) 87
SspMI CTAG 2 cut(s) 95, 261
StyI CCWWGG 2 cut(s) 19, 276
TaiI ACGT 1 cut(s) 217
TfiI GAWTC 3 cut(s) 5, 28, 257
Tru1I TTAA 3 cut(s) 11, 41, 252
Tru9I TTAA 3 cut(s) 11, 41, 252
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 2 cut(s) 77, 320
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 1 cut(s) 119
TspGWI ACGGA 2 cut(s) 79, 206
Vha464I CTTAAG 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 84
XspI CTAG 2 cut(s) 95, 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.