FvH4_5g32250

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008981
Physical Location & Seq
Reverse (-)
23265198 .. 23268338
3141 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32250.t1

Sequence Viewer

Length: 333 bp
ATGGATTCCGTTAGAGGACAAGGAGGCATCCAGATGCTGCTAACAGCAGAACAGGAAGCTCAACACATTGTCAACAGCGCTAGAAACTCGAAGATGGTGCGCTTGAGACAAGCTAAAGAAGAAGCTGATAGGGAAGTGCAACAGTATCGCACTAATATGGAGGCTGATTACCAAAACAAACTTTCTGAGACAAGTGGGAGTTCTGGATCCAATGTAAAGCGGCTCGAGGAAGAAACAGACACGAAGATTAAGAGCTTGAAGGAATCGGCCGCCAAAGTCCAATCAGATATTGTTGGCATGGTTATGAAGTATATCACTAATGTGAAAATCTGA

Protein Analysis

111

Amino Acids

12.37

Weight (kDa)

7.95

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-ATPase_G PF03179 7 - 109 5.9e-31 Vacuolar (H+)-ATPase G subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 220, 270
AclWI GGATC 2 cut(s) 201, 214
AcoI YGGCCR 1 cut(s) 267
AfeI AGCGCT 1 cut(s) 79
AgsI TTSAA 1 cut(s) 259
AleI CACNNNNGTG 1 cut(s) 320
AluBI AGCT 4 cut(s) 59, 113, 125, 255
AluI AGCT 4 cut(s) 59, 113, 125, 255
Alw26I GTCTC 2 cut(s) 100, 182
AlwI GGATC 2 cut(s) 201, 214
AlwNI CAGNNNCTG 1 cut(s) 37
Ama87I CYCGRG 1 cut(s) 224
Aor51HI AGCGCT 1 cut(s) 79
AoxI GGCC 1 cut(s) 267
ApeKI GCWGC 1 cut(s) 37
AspLEI GCGC 2 cut(s) 80, 102
AvaI CYCGRG 1 cut(s) 224
BamHI GGATCC 1 cut(s) 206
BbvI GCAGC 1 cut(s) 24
BccI CCATC 1 cut(s) 88
BcgI CGANNNNNNTGC 4 cut(s) 79, 113, 128, 162
BcoDI GTCTC 2 cut(s) 100, 182
BfaI CTAG 1 cut(s) 81
BfoI RGCGCY 1 cut(s) 81
BisI GCNGC 3 cut(s) 38, 221, 270
BlsI GCNGC 3 cut(s) 39, 222, 271
BmeT110I CYCGRG 1 cut(s) 224
BmiI GGNNCC 1 cut(s) 208
BmsI GCATC 2 cut(s) 24, 36
BpuEI CTTGAG 1 cut(s) 124
BseGI GGATG 1 cut(s) 27
BseMII CTCAG 1 cut(s) 177
BseX3I CGGCCG 1 cut(s) 267
BseXI GCAGC 1 cut(s) 24
Bsh1285I CGRYCG 1 cut(s) 270
BshFI GGCC 1 cut(s) 269
BsiEI CGRYCG 1 cut(s) 270
BsiHKCI CYCGRG 1 cut(s) 224
BsmAI GTCTC 2 cut(s) 100, 182
BsnI GGCC 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 224
Bsp143I GATC 1 cut(s) 206
BspACI CCGC 2 cut(s) 220, 270
BspANI GGCC 1 cut(s) 269
BspCNI CTCAG 1 cut(s) 178
BspLI GGNNCC 1 cut(s) 208
BspPI GGATC 2 cut(s) 201, 214
BssMI GATC 1 cut(s) 206
Bst4CI ACNGT 1 cut(s) 144
BstDEI CTNAG 1 cut(s) 186
BstF5I GGATG 1 cut(s) 27
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 2 cut(s) 80, 102
BstKTI GATC 1 cut(s) 209
BstMAI GTCTC 2 cut(s) 100, 182
BstMBI GATC 1 cut(s) 206
BstMCI CGRYCG 1 cut(s) 270
BstV1I GCAGC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
BstZI CGGCCG 1 cut(s) 267
BsuRI GGCC 1 cut(s) 269
BtsCI GGATG 1 cut(s) 27
CaiI CAGNNNCTG 1 cut(s) 37
CfoI GCGC 2 cut(s) 80, 102
CviAII CATG 1 cut(s) 298
CviJI RGCY 7 cut(s) 59, 113, 125, 164, 223, 255, 269
CviKI_1 RGCY 7 cut(s) 59, 113, 125, 164, 223, 255, 269
DdeI CTNAG 1 cut(s) 186
DpnI GATC 1 cut(s) 208
DpnII GATC 1 cut(s) 206
EaeI YGGCCR 1 cut(s) 267
EagI CGGCCG 1 cut(s) 267
EclXI CGGCCG 1 cut(s) 267
Eco47III AGCGCT 1 cut(s) 79
Eco52I CGGCCG 1 cut(s) 267
Eco88I CYCGRG 1 cut(s) 224
FaeI CATG 1 cut(s) 301
FaiI YATR 4 cut(s) 158, 299, 305, 312
FatI CATG 1 cut(s) 297
Fnu4HI GCNGC 3 cut(s) 38, 221, 270
FokI GGATG 1 cut(s) 14
Fsp4HI GCNGC 3 cut(s) 38, 221, 270
FspBI CTAG 1 cut(s) 81
GlaI GCGC 2 cut(s) 79, 101
GluI GCNGC 3 cut(s) 38, 221, 270
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 1 cut(s) 269
HhaI GCGC 2 cut(s) 80, 102
Hin1II CATG 1 cut(s) 301
Hin6I GCGC 2 cut(s) 78, 100
HinP1I GCGC 2 cut(s) 78, 100
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HinfI GANTC 2 cut(s) 5, 263
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 3 cut(s) 187, 286, 332
Hpy188III TCNNGA 2 cut(s) 31, 204
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 1 cut(s) 144
HpyCH4V TGCA 1 cut(s) 139
HpyF3I CTNAG 1 cut(s) 186
Hsp92II CATG 1 cut(s) 301
HspAI GCGC 2 cut(s) 78, 100
Kzo9I GATC 1 cut(s) 206
LpnPI CCDG 3 cut(s) 38, 44, 189
Lsp1109I GCAGC 1 cut(s) 24
LweI GCATC 2 cut(s) 24, 36
MaeI CTAG 1 cut(s) 81
MalI GATC 1 cut(s) 208
MboI GATC 1 cut(s) 206
MboII GAAGA 4 cut(s) 103, 131, 242, 256
MflI RGATCY 1 cut(s) 206
MnlI CCTC 4 cut(s) 8, 17, 154, 220
MseI TTAA 1 cut(s) 249
MslI CAYNNNNRTG 4 cut(s) 32, 155, 302, 320
NdeII GATC 1 cut(s) 206
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 1 cut(s) 208
OliI CACNNNNGTG 1 cut(s) 320
PaeR7I CTCGAG 1 cut(s) 224
PfeI GAWTC 2 cut(s) 5, 263
PkrI GCNGC 3 cut(s) 39, 222, 271
PspN4I GGNNCC 1 cut(s) 208
PspXI VCTCGAGB 1 cut(s) 224
PstNI CAGNNNCTG 1 cut(s) 37
PsuI RGATCY 1 cut(s) 206
RseI CAYNNNNRTG 4 cut(s) 32, 155, 302, 320
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 3 cut(s) 38, 221, 270
Sau3AI GATC 1 cut(s) 206
SetI ASST 4 cut(s) 61, 115, 127, 257
SfaNI GCATC 2 cut(s) 24, 36
Sfr274I CTCGAG 1 cut(s) 224
SlaI CTCGAG 1 cut(s) 224
SmiMI CAYNNNNRTG 4 cut(s) 32, 155, 302, 320
SmlI CTYRAG 2 cut(s) 103, 224
SmoI CTYRAG 2 cut(s) 103, 224
SsiI CCGC 2 cut(s) 220, 270
SspMI CTAG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 144
TaqI TCGA 2 cut(s) 89, 225
TauI GCSGC 2 cut(s) 223, 272
TfiI GAWTC 2 cut(s) 5, 263
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TseI GCWGC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 320
XhoI CTCGAG 1 cut(s) 224
XspI CTAG 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.