Rh7DG397100

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008981
Physical Location & Seq
Reverse (-)
54445962 .. 54446296
335 bp
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UTR
Exon/CDS
Intron
Rh7DG397100.1

Sequence Viewer

Length: 177 bp
ATGGAGGCTGAGTACCAAAACAAACTTTCGGAGACAAGTGGGAGTTCTGGATCCAATGTAAAAAGGCTCGAGGAAGAAACAGAAACGAAGATTAAGAGCTTGAAGGAATCGGCCTCCAAAGTCCAATCAGATATTGTTGGCATGGTTATGAAGTATATCACTGATGTGAAAATTTGA

Protein Analysis

58

Amino Acids

6.46

Weight (kDa)

5.34

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-ATPase_G PF03179 1 - 57 1.7e-09 Vacuolar (H+)-ATPase G subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 45, 58
AcsI RAATTY 1 cut(s) 171
AfaI GTAC 1 cut(s) 14
AgsI TTSAA 1 cut(s) 103
AleI CACNNNNGTG 1 cut(s) 164
AluBI AGCT 1 cut(s) 99
AluI AGCT 1 cut(s) 99
Alw26I GTCTC 1 cut(s) 26
AlwI GGATC 2 cut(s) 45, 58
Ama87I CYCGRG 1 cut(s) 68
AoxI GGCC 1 cut(s) 111
ApoI RAATTY 1 cut(s) 171
AvaI CYCGRG 1 cut(s) 68
BamHI GGATCC 1 cut(s) 50
BcoDI GTCTC 1 cut(s) 26
BmeT110I CYCGRG 1 cut(s) 68
BmiI GGNNCC 1 cut(s) 52
BsaXI ACNNNNNCTCC 1 cut(s) 26
BshFI GGCC 1 cut(s) 113
BsiHKCI CYCGRG 1 cut(s) 68
BsmAI GTCTC 1 cut(s) 26
BsnI GGCC 1 cut(s) 113
BsoBI CYCGRG 1 cut(s) 68
Bsp143I GATC 1 cut(s) 50
BspANI GGCC 1 cut(s) 113
BspLI GGNNCC 1 cut(s) 52
BspPI GGATC 2 cut(s) 45, 58
BssMI GATC 1 cut(s) 50
BstDEI CTNAG 1 cut(s) 9
BstKTI GATC 1 cut(s) 53
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 1 cut(s) 50
BstX2I RGATCY 1 cut(s) 50
BstYI RGATCY 1 cut(s) 50
BsuRI GGCC 1 cut(s) 113
BtsIMutI CAGTG 1 cut(s) 159
Csp6I GTAC 1 cut(s) 13
CviAII CATG 1 cut(s) 142
CviJI RGCY 4 cut(s) 8, 67, 99, 113
CviKI_1 RGCY 4 cut(s) 8, 67, 99, 113
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 1 cut(s) 9
DpnI GATC 1 cut(s) 52
DpnII GATC 1 cut(s) 50
Eco88I CYCGRG 1 cut(s) 68
FaeI CATG 1 cut(s) 145
FaiI YATR 3 cut(s) 143, 149, 156
FatI CATG 1 cut(s) 141
HaeIII GGCC 1 cut(s) 113
Hin1II CATG 1 cut(s) 145
HinfI GANTC 1 cut(s) 107
Hpy188I TCNGA 2 cut(s) 31, 130
Hpy188III TCNNGA 1 cut(s) 48
HpyAV CCTTC 1 cut(s) 97
HpyF3I CTNAG 1 cut(s) 9
Hsp92II CATG 1 cut(s) 145
Kzo9I GATC 1 cut(s) 50
LpnPI CCDG 1 cut(s) 33
MalI GATC 1 cut(s) 52
MboI GATC 1 cut(s) 50
MboII GAAGA 2 cut(s) 86, 100
MflI RGATCY 1 cut(s) 50
MluCI AATT 1 cut(s) 171
MnlI CCTC 2 cut(s) 64, 124
MseI TTAA 1 cut(s) 93
MslI CAYNNNNRTG 2 cut(s) 146, 164
NdeII GATC 1 cut(s) 50
NlaIII CATG 1 cut(s) 145
NlaIV GGNNCC 1 cut(s) 52
OliI CACNNNNGTG 1 cut(s) 164
PaeR7I CTCGAG 1 cut(s) 68
PfeI GAWTC 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 52
PspXI VCTCGAGB 1 cut(s) 68
PsuI RGATCY 1 cut(s) 50
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
RseI CAYNNNNRTG 2 cut(s) 146, 164
SaqAI TTAA 1 cut(s) 93
Sau3AI GATC 1 cut(s) 50
SetI ASST 1 cut(s) 101
Sfr274I CTCGAG 1 cut(s) 68
SgeI CNNG 6 cut(s) 48, 60, 80, 82, 112, 154
SlaI CTCGAG 1 cut(s) 68
SmiMI CAYNNNNRTG 2 cut(s) 146, 164
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse9I AATT 1 cut(s) 171
TaqI TCGA 1 cut(s) 69
TasI AATT 1 cut(s) 171
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TscAI CASTG 1 cut(s) 166
TspDTI ATGAA 1 cut(s) 164
TspRI CASTG 1 cut(s) 166
XapI RAATTY 1 cut(s) 171
XhoI CTCGAG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.