Rroxscaffold_3G00231220

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0008981
Physical Location & Seq
Forward (+)
16244981 .. 16245997
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00231220.1

Sequence Viewer

Length: 438 bp
ATGCTAGCTGCAGCTACTGTATCGTATTATATAGCACTCAGAATATTCCAAAAGCTTATTAAGTCTGGTCAGAATGGATTAAGTTATCCATTCATAAAGTACCTAAGGTTAGCAATAGGTTGTACAGAGATGGATTCCGTTAGAGGACAAGGAGGCATCCAGATGCTGCTAACAGCTGAACAGGAAGCTCAACACATTATGGCTCGCTTGAGACAAGCTAAAGAAGAAGCTGAGAGGGAAGTGCAACAGTATCGCACCAATATGGAGGCTGAGTACCAAAACAAACTTTCAGAGACAAGTGGGAGTTCTGGATCCAATGTAAAAAGGCTCGAGGAAGAAACAGAAACGAAGATTAAGAGCTTGAAGGAATCAGCCTCCAAAGTCCAATCAGATATTGTTGGCATGGTTATGAAGTATATCACTAATGTGAAAATTTGA

Protein Analysis

145

Amino Acids

16.35

Weight (kDa)

8.94

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-ATPase_G PF03179 50 - 144 2.3e-22 Vacuolar (H+)-ATPase G subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 306, 319
AcsI RAATTY 1 cut(s) 432
AfaI GTAC 3 cut(s) 101, 124, 275
AgsI TTSAA 1 cut(s) 364
AleI CACNNNNGTG 1 cut(s) 425
AluBI AGCT 8 cut(s) 8, 14, 55, 176, 188, 218, 230, 360
AluI AGCT 8 cut(s) 8, 14, 55, 176, 188, 218, 230, 360
Alw26I GTCTC 2 cut(s) 205, 287
AlwI GGATC 2 cut(s) 306, 319
AlwNI CAGNNNCTG 2 cut(s) 17, 166
Ama87I CYCGRG 1 cut(s) 329
ApeKI GCWGC 3 cut(s) 8, 11, 166
ApoI RAATTY 1 cut(s) 432
AsuNHI GCTAGC 1 cut(s) 4
AvaI CYCGRG 1 cut(s) 329
AxyI CCTNAGG 1 cut(s) 104
BamHI GGATCC 1 cut(s) 311
BbvI GCAGC 2 cut(s) 23, 153
BccI CCATC 1 cut(s) 124
BcgI CGANNNNNNTGC 2 cut(s) 233, 267
BcoDI GTCTC 2 cut(s) 205, 287
BfaI CTAG 1 cut(s) 5
BfmI CTRYAG 1 cut(s) 9
BisI GCNGC 3 cut(s) 9, 12, 167
BlsI GCNGC 3 cut(s) 10, 13, 168
BmeT110I CYCGRG 1 cut(s) 329
BmiI GGNNCC 1 cut(s) 313
BmsI GCATC 2 cut(s) 153, 165
BmtI GCTAGC 1 cut(s) 8
BpuEI CTTGAG 1 cut(s) 229
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bse21I CCTNAGG 1 cut(s) 104
BseGI GGATG 1 cut(s) 156
BseMII CTCAG 3 cut(s) 52, 222, 261
BseXI GCAGC 2 cut(s) 23, 153
BsiHKCI CYCGRG 1 cut(s) 329
BsmAI GTCTC 2 cut(s) 205, 287
BsoBI CYCGRG 1 cut(s) 329
Bsp1407I TGTACA 1 cut(s) 122
Bsp143I GATC 1 cut(s) 311
BspCNI CTCAG 3 cut(s) 51, 223, 262
BspLI GGNNCC 1 cut(s) 313
BspMAI CTGCAG 1 cut(s) 13
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 2 cut(s) 306, 319
BsrGI TGTACA 1 cut(s) 122
BssMI GATC 1 cut(s) 311
Bst4CI ACNGT 2 cut(s) 19, 249
BstAUI TGTACA 1 cut(s) 122
BstC8I GCNNGC 2 cut(s) 6, 205
BstDEI CTNAG 4 cut(s) 38, 104, 231, 270
BstF5I GGATG 1 cut(s) 156
BstKTI GATC 1 cut(s) 314
BstMAI GTCTC 2 cut(s) 205, 287
BstMBI GATC 1 cut(s) 311
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 2 cut(s) 23, 153
BstX2I RGATCY 1 cut(s) 311
BstYI RGATCY 1 cut(s) 311
Bsu36I CCTNAGG 1 cut(s) 104
BtsCI GGATG 1 cut(s) 156
Cac8I GCNNGC 2 cut(s) 6, 205
CaiI CAGNNNCTG 2 cut(s) 17, 166
Csp6I GTAC 3 cut(s) 100, 123, 274
CviAII CATG 1 cut(s) 403
CviQI GTAC 3 cut(s) 100, 123, 274
DdeI CTNAG 4 cut(s) 38, 104, 231, 270
DpnI GATC 1 cut(s) 313
DpnII GATC 1 cut(s) 311
Eco81I CCTNAGG 1 cut(s) 104
Eco88I CYCGRG 1 cut(s) 329
FaeI CATG 1 cut(s) 406
FaiI YATR 8 cut(s) 30, 32, 95, 200, 263, 404, 410, 417
FatI CATG 1 cut(s) 402
Fnu4HI GCNGC 3 cut(s) 9, 12, 167
FokI GGATG 1 cut(s) 143
Fsp4HI GCNGC 3 cut(s) 9, 12, 167
FspBI CTAG 1 cut(s) 5
GluI GCNGC 3 cut(s) 9, 12, 167
Hin1II CATG 1 cut(s) 406
HindIII AAGCTT 1 cut(s) 53
HinfI GANTC 2 cut(s) 134, 368
Hpy188I TCNGA 4 cut(s) 41, 72, 292, 391
Hpy188III TCNNGA 2 cut(s) 160, 309
HpyAV CCTTC 1 cut(s) 358
HpyCH4III ACNGT 2 cut(s) 19, 249
HpyCH4V TGCA 2 cut(s) 11, 244
HpyF3I CTNAG 4 cut(s) 38, 104, 231, 270
Hsp92II CATG 1 cut(s) 406
Kzo9I GATC 1 cut(s) 311
LpnPI CCDG 4 cut(s) 51, 167, 173, 294
Lsp1109I GCAGC 2 cut(s) 23, 153
LweI GCATC 2 cut(s) 153, 165
MaeI CTAG 1 cut(s) 5
MalI GATC 1 cut(s) 313
MboI GATC 1 cut(s) 311
MboII GAAGA 3 cut(s) 236, 347, 361
MflI RGATCY 1 cut(s) 311
MluCI AATT 1 cut(s) 432
MnlI CCTC 6 cut(s) 137, 146, 228, 259, 325, 385
MseI TTAA 3 cut(s) 60, 80, 354
MslI CAYNNNNRTG 4 cut(s) 161, 260, 407, 425
MspA1I CMGCKG 1 cut(s) 176
NdeII GATC 1 cut(s) 311
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 1 cut(s) 406
NlaIV GGNNCC 1 cut(s) 313
OliI CACNNNNGTG 1 cut(s) 425
PaeR7I CTCGAG 1 cut(s) 329
PfeI GAWTC 2 cut(s) 134, 368
PkrI GCNGC 3 cut(s) 10, 13, 168
PspN4I GGNNCC 1 cut(s) 313
PspXI VCTCGAGB 1 cut(s) 329
PstI CTGCAG 1 cut(s) 13
PstNI CAGNNNCTG 2 cut(s) 17, 166
PsuI RGATCY 1 cut(s) 311
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 3 cut(s) 101, 124, 275
RsaNI GTAC 3 cut(s) 100, 123, 274
RseI CAYNNNNRTG 4 cut(s) 161, 260, 407, 425
SaqAI TTAA 3 cut(s) 60, 80, 354
SatI GCNGC 3 cut(s) 9, 12, 167
Sau3AI GATC 1 cut(s) 311
SfaNI GCATC 2 cut(s) 153, 165
SfcI CTRYAG 1 cut(s) 9
Sfr274I CTCGAG 1 cut(s) 329
SlaI CTCGAG 1 cut(s) 329
SmiMI CAYNNNNRTG 4 cut(s) 161, 260, 407, 425
SmlI CTYRAG 2 cut(s) 208, 329
SmoI CTYRAG 2 cut(s) 208, 329
Sse9I AATT 1 cut(s) 432
SspI AATATT 1 cut(s) 45
SspMI CTAG 1 cut(s) 5
TaaI ACNGT 2 cut(s) 19, 249
TaqI TCGA 1 cut(s) 330
TasI AATT 1 cut(s) 432
TatI WGTACW 1 cut(s) 122
TfiI GAWTC 2 cut(s) 134, 368
Tru1I TTAA 3 cut(s) 60, 80, 354
Tru9I TTAA 3 cut(s) 60, 80, 354
TseI GCWGC 3 cut(s) 8, 11, 166
TspDTI ATGAA 2 cut(s) 82, 425
TspGWI ACGGA 1 cut(s) 127
XapI RAATTY 1 cut(s) 432
XhoI CTCGAG 1 cut(s) 329
XspI CTAG 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.