Rorug07G0247800

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008981
Physical Location & Seq
Reverse (-)
22919307 .. 22919760
454 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0247800.1

Sequence Viewer

Length: 285 bp
ATGGGATTCCGGTTGCCTGGAATTGTTAACACCAAGAAAAGTCTTATTCAACCTATATCTGCTGCTAAGACCTTAGATGTCCCGAAAGGTTATCTCGCGGTCTATGTTGGGAAGAAACAGATGAAGAGATTTGTAATCCCAGTATCATATTTGAACCAACCTCTGTTCCTGGATTTGCTGAGTCAAGCTGAAGAAGAATTTGGATACGATCATCCAATGGGTGGTCTCACAATTCCTTGCAGCAAAGACACCTTCCTTGATCTCACTTCACGCCTGAGTGTGTAA

Protein Analysis

94

Amino Acids

10.45

Weight (kDa)

9.13

Isoelectric Point (pI)

29.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 21 - 91 3.1e-22 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 221
AccII CGCG 1 cut(s) 98
AciI CCGC 1 cut(s) 98
AcsI RAATTY 1 cut(s) 197
AcuI CTGAAG 1 cut(s) 210
AfiI CCNNNNNNNGG 1 cut(s) 221
AgsI TTSAA 2 cut(s) 50, 154
AjnI CCWGG 2 cut(s) 16, 168
AjuI GAANNNNNNNTTGG 2 cut(s) 183, 215
AluBI AGCT 1 cut(s) 188
AluI AGCT 1 cut(s) 188
Alw26I GTCTC 1 cut(s) 230
ApeKI GCWGC 2 cut(s) 62, 240
ApoI RAATTY 1 cut(s) 197
BbvI GCAGC 2 cut(s) 49, 252
BciT130I CCWGG 2 cut(s) 18, 170
BciVI GTATCC 1 cut(s) 197
BcoDI GTCTC 1 cut(s) 230
BfuI GTATCC 1 cut(s) 197
BisI GCNGC 2 cut(s) 63, 241
BlsI GCNGC 2 cut(s) 64, 242
Bme1390I CCNGG 2 cut(s) 18, 170
BmrFI CCNGG 2 cut(s) 18, 170
BmrI ACTGGG 1 cut(s) 134
BmuI ACTGGG 1 cut(s) 134
BsaI GGTCTC 1 cut(s) 230
BsaWI WCCGGW 1 cut(s) 9
Bsc4I CCNNNNNNNGG 1 cut(s) 221
Bse1I ACTGG 1 cut(s) 140
BseBI CCWGG 2 cut(s) 18, 170
BseGI GGATG 1 cut(s) 211
BseLI CCNNNNNNNGG 1 cut(s) 221
BseMII CTCAG 2 cut(s) 170, 266
BseNI ACTGG 1 cut(s) 140
BseXI GCAGC 2 cut(s) 49, 252
Bsh1236I CGCG 1 cut(s) 98
BsiSI CCGG 1 cut(s) 10
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 1 cut(s) 221
BsmAI GTCTC 1 cut(s) 230
BsmFI GGGAC 1 cut(s) 65
Bso31I GGTCTC 1 cut(s) 230
Bsp143I GATC 2 cut(s) 208, 259
BspACI CCGC 1 cut(s) 98
BspCNI CTCAG 2 cut(s) 171, 267
BspFNI CGCG 1 cut(s) 98
BspTNI GGTCTC 1 cut(s) 230
BsrI ACTGG 1 cut(s) 140
BssMI GATC 2 cut(s) 208, 259
Bst2UI CCWGG 2 cut(s) 18, 170
Bst6I CTCTTC 1 cut(s) 119
BstDEI CTNAG 4 cut(s) 66, 73, 179, 275
BstF5I GGATG 1 cut(s) 211
BstFNI CGCG 1 cut(s) 98
BstKTI GATC 2 cut(s) 211, 262
BstMAI GTCTC 1 cut(s) 230
BstMBI GATC 2 cut(s) 208, 259
BstNI CCWGG 2 cut(s) 18, 170
BstSCI CCNGG 2 cut(s) 16, 168
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 2 cut(s) 49, 252
BsuI GTATCC 1 cut(s) 197
BtsCI GGATG 1 cut(s) 211
CviJI RGCY 1 cut(s) 188
CviKI_1 RGCY 1 cut(s) 188
DdeI CTNAG 4 cut(s) 66, 73, 179, 275
DpnI GATC 2 cut(s) 210, 261
DpnII GATC 2 cut(s) 208, 259
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
Eco31I GGTCTC 1 cut(s) 230
Eco57I CTGAAG 1 cut(s) 210
EcoRII CCWGG 2 cut(s) 16, 168
FaiI YATR 3 cut(s) 56, 105, 148
FaqI GGGAC 1 cut(s) 65
Fnu4HI GCNGC 2 cut(s) 63, 241
FokI GGATG 1 cut(s) 198
Fsp4HI GCNGC 2 cut(s) 63, 241
GluI GCNGC 2 cut(s) 63, 241
HapII CCGG 1 cut(s) 10
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HinfI GANTC 2 cut(s) 6, 181
HpaI GTTAAC 1 cut(s) 28
HpaII CCGG 1 cut(s) 10
Hpy166II GTNNAC 1 cut(s) 28
Hpy188III TCNNGA 1 cut(s) 82
Hpy8I GTNNAC 1 cut(s) 28
HpyAV CCTTC 1 cut(s) 262
HpyCH4V TGCA 1 cut(s) 240
HpyF3I CTNAG 4 cut(s) 66, 73, 179, 275
KspAI GTTAAC 1 cut(s) 28
Kzo9I GATC 2 cut(s) 208, 259
LpnPI CCDG 6 cut(s) 3, 23, 30, 153, 155, 182
Lsp1109I GCAGC 2 cut(s) 49, 252
MalI GATC 2 cut(s) 210, 261
MboI GATC 2 cut(s) 208, 259
MboII GAAGA 4 cut(s) 124, 136, 203, 206
MluCI AATT 3 cut(s) 21, 197, 231
MlyI GAGTC 1 cut(s) 190
MnlI CCTC 1 cut(s) 171
MseI TTAA 1 cut(s) 27
MspI CCGG 1 cut(s) 10
MspR9I CCNGG 2 cut(s) 18, 170
MvaI CCWGG 2 cut(s) 18, 170
MvnI CGCG 1 cut(s) 98
NdeII GATC 2 cut(s) 208, 259
PfeI GAWTC 1 cut(s) 6
PflMI CCANNNNNTGG 1 cut(s) 221
PfoI TCCNGGA 1 cut(s) 168
PkrI GCNGC 2 cut(s) 64, 242
PleI GAGTC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 189
Psp6I CCWGG 2 cut(s) 16, 168
PspGI CCWGG 2 cut(s) 16, 168
SaqAI TTAA 1 cut(s) 27
SatI GCNGC 2 cut(s) 63, 241
Sau3AI GATC 2 cut(s) 208, 259
SchI GAGTC 1 cut(s) 190
ScrFI CCNGG 2 cut(s) 18, 170
SetI ASST 6 cut(s) 55, 74, 91, 163, 190, 254
Sse9I AATT 3 cut(s) 21, 197, 231
SsiI CCGC 1 cut(s) 98
StyD4I CCNGG 2 cut(s) 16, 168
TasI AATT 3 cut(s) 21, 197, 231
TfiI GAWTC 1 cut(s) 6
Tru1I TTAA 1 cut(s) 27
Tru9I TTAA 1 cut(s) 27
TseI GCWGC 2 cut(s) 62, 240
TspDTI ATGAA 1 cut(s) 137
Van91I CCANNNNNTGG 1 cut(s) 221
XapI RAATTY 1 cut(s) 197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.