Rh7AG401600

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0008981
Physical Location & Seq
Reverse (-)
52841476 .. 52845094
3619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG401600.1

Sequence Viewer

Length: 333 bp
ATGGATTCCGTTAGAGGACAAGGAGGCATCCAGATGCTGCTAACAGCTGAACAGGAAGCTCAACACATTGTTAACAGCGCTAGAAACTTGAAGATGGCTCGCTTGAGACAAGCTAAAGAAGAAGCTGAGAGGGAAGTGCAACAGTATCGCACCAATATGGAGGCTGAGTACCAAAACAAACTTTCAGAGACAAGTGGGAGTTCTGGATCCAATGTAAAAAGGCTCGAGGAAGAAACAGAAACAAAGATTAAGAGCTTGAAGGAATCAGCCTCCAAAGTCCAATCAGATATTGTTGGCATGGTTATGAAGTATATCACTAATGTGAAAATTTGA

Protein Analysis

110

Amino Acids

12.42

Weight (kDa)

7.95

Isoelectric Point (pI)

41.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-ATPase_G PF03179 7 - 109 1.8e-31 Vacuolar (H+)-ATPase G subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 201, 214
AcsI RAATTY 1 cut(s) 327
AfaI GTAC 1 cut(s) 170
AfeI AGCGCT 1 cut(s) 79
AgsI TTSAA 2 cut(s) 91, 259
AleI CACNNNNGTG 1 cut(s) 320
AluBI AGCT 5 cut(s) 47, 59, 113, 125, 255
AluI AGCT 5 cut(s) 47, 59, 113, 125, 255
Alw26I GTCTC 2 cut(s) 100, 182
AlwI GGATC 2 cut(s) 201, 214
AlwNI CAGNNNCTG 1 cut(s) 37
Ama87I CYCGRG 1 cut(s) 224
Aor51HI AGCGCT 1 cut(s) 79
ApeKI GCWGC 1 cut(s) 37
ApoI RAATTY 1 cut(s) 327
AspLEI GCGC 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 224
BamHI GGATCC 1 cut(s) 206
BbvI GCAGC 1 cut(s) 24
BccI CCATC 1 cut(s) 88
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BcoDI GTCTC 2 cut(s) 100, 182
BfaI CTAG 1 cut(s) 81
BfoI RGCGCY 1 cut(s) 81
BisI GCNGC 1 cut(s) 38
BlsI GCNGC 1 cut(s) 39
BmeT110I CYCGRG 1 cut(s) 224
BmiI GGNNCC 1 cut(s) 208
BmsI GCATC 2 cut(s) 24, 36
BpuEI CTTGAG 1 cut(s) 124
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
BseGI GGATG 1 cut(s) 27
BseMII CTCAG 2 cut(s) 117, 156
BseXI GCAGC 1 cut(s) 24
BsiHKCI CYCGRG 1 cut(s) 224
BsmAI GTCTC 2 cut(s) 100, 182
BsoBI CYCGRG 1 cut(s) 224
Bsp143I GATC 1 cut(s) 206
BspCNI CTCAG 2 cut(s) 118, 157
BspLI GGNNCC 1 cut(s) 208
BspPI GGATC 2 cut(s) 201, 214
BssMI GATC 1 cut(s) 206
Bst4CI ACNGT 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 100
BstDEI CTNAG 2 cut(s) 126, 165
BstF5I GGATG 1 cut(s) 27
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 1 cut(s) 209
BstMAI GTCTC 2 cut(s) 100, 182
BstMBI GATC 1 cut(s) 206
BstV1I GCAGC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 206
BstYI RGATCY 1 cut(s) 206
BtsCI GGATG 1 cut(s) 27
Cac8I GCNNGC 1 cut(s) 100
CaiI CAGNNNCTG 1 cut(s) 37
CfoI GCGC 1 cut(s) 80
Csp6I GTAC 1 cut(s) 169
CviAII CATG 1 cut(s) 298
CviJI RGCY 9 cut(s) 47, 59, 98, 113, 125, 164, 223, 255, 269
CviKI_1 RGCY 9 cut(s) 47, 59, 98, 113, 125, 164, 223, 255, 269
CviQI GTAC 1 cut(s) 169
DdeI CTNAG 2 cut(s) 126, 165
DpnI GATC 1 cut(s) 208
DpnII GATC 1 cut(s) 206
Eco47III AGCGCT 1 cut(s) 79
Eco88I CYCGRG 1 cut(s) 224
FaeI CATG 1 cut(s) 301
FaiI YATR 4 cut(s) 158, 299, 305, 312
FatI CATG 1 cut(s) 297
Fnu4HI GCNGC 1 cut(s) 38
FokI GGATG 1 cut(s) 14
Fsp4HI GCNGC 1 cut(s) 38
FspBI CTAG 1 cut(s) 81
GlaI GCGC 1 cut(s) 79
GluI GCNGC 1 cut(s) 38
HaeII RGCGCY 1 cut(s) 81
HhaI GCGC 1 cut(s) 80
Hin1II CATG 1 cut(s) 301
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HinfI GANTC 2 cut(s) 5, 263
HpaI GTTAAC 1 cut(s) 73
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 2 cut(s) 187, 286
Hpy188III TCNNGA 2 cut(s) 31, 204
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 1 cut(s) 253
HpyCH4III ACNGT 1 cut(s) 144
HpyCH4V TGCA 1 cut(s) 139
HpyF3I CTNAG 2 cut(s) 126, 165
Hsp92II CATG 1 cut(s) 301
HspAI GCGC 1 cut(s) 78
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 1 cut(s) 206
LpnPI CCDG 3 cut(s) 38, 44, 189
Lsp1109I GCAGC 1 cut(s) 24
LweI GCATC 2 cut(s) 24, 36
MaeI CTAG 1 cut(s) 81
MalI GATC 1 cut(s) 208
MboI GATC 1 cut(s) 206
MboII GAAGA 3 cut(s) 103, 131, 242
MflI RGATCY 1 cut(s) 206
MluCI AATT 1 cut(s) 327
MnlI CCTC 6 cut(s) 8, 17, 123, 154, 220, 280
MseI TTAA 2 cut(s) 72, 249
MslI CAYNNNNRTG 4 cut(s) 32, 155, 302, 320
MspA1I CMGCKG 1 cut(s) 47
NdeII GATC 1 cut(s) 206
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 1 cut(s) 208
OliI CACNNNNGTG 1 cut(s) 320
PaeR7I CTCGAG 1 cut(s) 224
PfeI GAWTC 2 cut(s) 5, 263
PkrI GCNGC 1 cut(s) 39
PspN4I GGNNCC 1 cut(s) 208
PspXI VCTCGAGB 1 cut(s) 224
PstNI CAGNNNCTG 1 cut(s) 37
PsuI RGATCY 1 cut(s) 206
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 1 cut(s) 170
RsaNI GTAC 1 cut(s) 169
RseI CAYNNNNRTG 4 cut(s) 32, 155, 302, 320
SaqAI TTAA 2 cut(s) 72, 249
SatI GCNGC 1 cut(s) 38
Sau3AI GATC 1 cut(s) 206
SetI ASST 5 cut(s) 49, 61, 115, 127, 257
SfaNI GCATC 2 cut(s) 24, 36
Sfr274I CTCGAG 1 cut(s) 224
SlaI CTCGAG 1 cut(s) 224
SmiMI CAYNNNNRTG 4 cut(s) 32, 155, 302, 320
SmlI CTYRAG 2 cut(s) 103, 224
SmoI CTYRAG 2 cut(s) 103, 224
Sse9I AATT 1 cut(s) 327
SspMI CTAG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 144
TaqI TCGA 1 cut(s) 225
TasI AATT 1 cut(s) 327
TfiI GAWTC 2 cut(s) 5, 263
Tru1I TTAA 2 cut(s) 72, 249
Tru9I TTAA 2 cut(s) 72, 249
TseI GCWGC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 320
XapI RAATTY 1 cut(s) 327
XhoI CTCGAG 1 cut(s) 224
XspI CTAG 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.