Rh7DG397700

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008981
Physical Location & Seq
Reverse (-)
54558282 .. 54560963
2682 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG397700.1

Sequence Viewer

Length: 423 bp
ATGGATGGAATCGGAATTTTGTATGCAAGTCGCAGGCCTAGTTGCAAGTATGATAAAATAGCAATATCTTCTCCTTCAGGTTGTACAGAGATGGATTCCGTTAGAGGACAAGGAGGCATCCAGATGCTGCTAACAGCTGAACAGGAAGCTCAACACATTGTTAACAGCGCTAGAAACTTGAAGATGGCTCGCTTGAGACAAGCTAAAGAAGAAGCTGAGAGGGAAGTGCAACAGTATCGCACCAATATGGAGGCTGAGTACCAAAACAAACTTTCAGAGACAAGTGGGAGTTCTGGATCCAATGTAAAAAGGCTCGAGGAAGAAACAGAAACAAAGATTAAGAGCTTGAAGGAATCAGCCTCCAAAGTCCAATCAGATATTGTTGGCATGGTTATGAAGTATATCACTAATGTGAAAATTTGA

Protein Analysis

140

Amino Acids

15.62

Weight (kDa)

8.51

Isoelectric Point (pI)

56.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-ATPase_G PF03179 37 - 139 4.3e-31 Vacuolar (H+)-ATPase G subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 291, 304
AcsI RAATTY 2 cut(s) 15, 417
AcuI CTGAAG 1 cut(s) 60
AfaI GTAC 2 cut(s) 85, 260
AfeI AGCGCT 1 cut(s) 169
AgsI TTSAA 2 cut(s) 181, 349
AleI CACNNNNGTG 1 cut(s) 410
AluBI AGCT 5 cut(s) 137, 149, 203, 215, 345
AluI AGCT 5 cut(s) 137, 149, 203, 215, 345
Alw26I GTCTC 2 cut(s) 190, 272
AlwI GGATC 2 cut(s) 291, 304
AlwNI CAGNNNCTG 1 cut(s) 127
Ama87I CYCGRG 1 cut(s) 314
Aor51HI AGCGCT 1 cut(s) 169
AoxI GGCC 1 cut(s) 35
ApeKI GCWGC 1 cut(s) 127
ApoI RAATTY 2 cut(s) 15, 417
AspLEI GCGC 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 314
BamHI GGATCC 1 cut(s) 296
BbvI GCAGC 1 cut(s) 114
BccI CCATC 2 cut(s) 85, 178
BcgI CGANNNNNNTGC 2 cut(s) 218, 252
BcoDI GTCTC 2 cut(s) 190, 272
BfaI CTAG 2 cut(s) 39, 171
BfoI RGCGCY 1 cut(s) 171
BisI GCNGC 1 cut(s) 128
BlsI GCNGC 1 cut(s) 129
BmeT110I CYCGRG 1 cut(s) 314
BmiI GGNNCC 1 cut(s) 298
BmsI GCATC 2 cut(s) 114, 126
BpuEI CTTGAG 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
BseGI GGATG 2 cut(s) 10, 117
BseMII CTCAG 2 cut(s) 207, 246
BseXI GCAGC 1 cut(s) 114
BshFI GGCC 1 cut(s) 37
BsiHKCI CYCGRG 1 cut(s) 314
BsmAI GTCTC 2 cut(s) 190, 272
BsnI GGCC 1 cut(s) 37
BsoBI CYCGRG 1 cut(s) 314
Bsp1407I TGTACA 1 cut(s) 83
Bsp143I GATC 1 cut(s) 296
BspANI GGCC 1 cut(s) 37
BspCNI CTCAG 2 cut(s) 208, 247
BspLI GGNNCC 1 cut(s) 298
BspPI GGATC 2 cut(s) 291, 304
BsrGI TGTACA 1 cut(s) 83
BssMI GATC 1 cut(s) 296
Bst4CI ACNGT 1 cut(s) 234
BstAUI TGTACA 1 cut(s) 83
BstC8I GCNNGC 2 cut(s) 35, 190
BstDEI CTNAG 2 cut(s) 216, 255
BstF5I GGATG 2 cut(s) 10, 117
BstH2I RGCGCY 1 cut(s) 171
BstHHI GCGC 1 cut(s) 170
BstKTI GATC 1 cut(s) 299
BstMAI GTCTC 2 cut(s) 190, 272
BstMBI GATC 1 cut(s) 296
BstV1I GCAGC 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 296
BstYI RGATCY 1 cut(s) 296
BsuRI GGCC 1 cut(s) 37
BtsCI GGATG 2 cut(s) 10, 117
Cac8I GCNNGC 2 cut(s) 35, 190
CaiI CAGNNNCTG 1 cut(s) 127
CfoI GCGC 1 cut(s) 170
Csp6I GTAC 2 cut(s) 84, 259
CviAII CATG 1 cut(s) 388
CviQI GTAC 2 cut(s) 84, 259
DdeI CTNAG 2 cut(s) 216, 255
DpnI GATC 1 cut(s) 298
DpnII GATC 1 cut(s) 296
Eco147I AGGCCT 1 cut(s) 37
Eco47III AGCGCT 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 60
Eco88I CYCGRG 1 cut(s) 314
FaeI CATG 1 cut(s) 391
FaiI YATR 6 cut(s) 24, 51, 248, 389, 395, 402
FatI CATG 1 cut(s) 387
Fnu4HI GCNGC 1 cut(s) 128
FokI GGATG 2 cut(s) 17, 104
Fsp4HI GCNGC 1 cut(s) 128
FspBI CTAG 2 cut(s) 39, 171
GlaI GCGC 1 cut(s) 169
GluI GCNGC 1 cut(s) 128
HaeII RGCGCY 1 cut(s) 171
HaeIII GGCC 1 cut(s) 37
HhaI GCGC 1 cut(s) 170
Hin1II CATG 1 cut(s) 391
Hin6I GCGC 1 cut(s) 168
HinP1I GCGC 1 cut(s) 168
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 3 cut(s) 9, 95, 353
HpaI GTTAAC 1 cut(s) 163
Hpy166II GTNNAC 1 cut(s) 163
Hpy188I TCNGA 3 cut(s) 14, 277, 376
Hpy188III TCNNGA 2 cut(s) 121, 294
Hpy8I GTNNAC 1 cut(s) 163
HpyAV CCTTC 2 cut(s) 84, 343
HpyCH4III ACNGT 1 cut(s) 234
HpyCH4V TGCA 3 cut(s) 26, 45, 229
HpyF3I CTNAG 2 cut(s) 216, 255
Hsp92II CATG 1 cut(s) 391
HspAI GCGC 1 cut(s) 168
KspAI GTTAAC 1 cut(s) 163
Kzo9I GATC 1 cut(s) 296
LpnPI CCDG 5 cut(s) 19, 63, 128, 134, 279
Lsp1109I GCAGC 1 cut(s) 114
LweI GCATC 2 cut(s) 114, 126
MaeI CTAG 2 cut(s) 39, 171
MalI GATC 1 cut(s) 298
MboI GATC 1 cut(s) 296
MboII GAAGA 4 cut(s) 60, 193, 221, 332
MflI RGATCY 1 cut(s) 296
MluCI AATT 2 cut(s) 15, 417
MnlI CCTC 6 cut(s) 98, 107, 213, 244, 310, 370
MseI TTAA 2 cut(s) 162, 339
MslI CAYNNNNRTG 4 cut(s) 122, 245, 392, 410
MspA1I CMGCKG 1 cut(s) 137
NdeII GATC 1 cut(s) 296
NlaIII CATG 1 cut(s) 391
NlaIV GGNNCC 1 cut(s) 298
OliI CACNNNNGTG 1 cut(s) 410
PaeR7I CTCGAG 1 cut(s) 314
PceI AGGCCT 1 cut(s) 37
PfeI GAWTC 3 cut(s) 9, 95, 353
PkrI GCNGC 1 cut(s) 129
PspN4I GGNNCC 1 cut(s) 298
PspXI VCTCGAGB 1 cut(s) 314
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 1 cut(s) 296
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 2 cut(s) 85, 260
RsaNI GTAC 2 cut(s) 84, 259
RseI CAYNNNNRTG 4 cut(s) 122, 245, 392, 410
SaqAI TTAA 2 cut(s) 162, 339
SatI GCNGC 1 cut(s) 128
Sau3AI GATC 1 cut(s) 296
SetI ASST 6 cut(s) 82, 139, 151, 205, 217, 347
SfaNI GCATC 2 cut(s) 114, 126
Sfr274I CTCGAG 1 cut(s) 314
SlaI CTCGAG 1 cut(s) 314
SmiMI CAYNNNNRTG 4 cut(s) 122, 245, 392, 410
SmlI CTYRAG 2 cut(s) 193, 314
SmoI CTYRAG 2 cut(s) 193, 314
Sse9I AATT 2 cut(s) 15, 417
SseBI AGGCCT 1 cut(s) 37
SspMI CTAG 2 cut(s) 39, 171
StuI AGGCCT 1 cut(s) 37
TaaI ACNGT 1 cut(s) 234
TaqI TCGA 1 cut(s) 315
TasI AATT 2 cut(s) 15, 417
TatI WGTACW 1 cut(s) 83
TfiI GAWTC 3 cut(s) 9, 95, 353
Tru1I TTAA 2 cut(s) 162, 339
Tru9I TTAA 2 cut(s) 162, 339
TseI GCWGC 1 cut(s) 127
TspDTI ATGAA 1 cut(s) 410
TspGWI ACGGA 1 cut(s) 88
XapI RAATTY 2 cut(s) 15, 417
XhoI CTCGAG 1 cut(s) 314
XspI CTAG 2 cut(s) 39, 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.