MD15G1410200.v1.1

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008981
Physical Location & Seq
Forward (+)
50985361 .. 50987026
1666 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1410200.v1.1.491

Sequence Viewer

Length: 333 bp
ATGGATTCGGTTAGAGGACAAGGAGGCATCCAGATGTTGCTAACAGCAGAACAGGATGCTCAACACATTGTGAACAATGCTAGAAACTCAAAGATGGCACGCTTGAGACAAGCTAAAGAAGAAGCTGATAGGGAAGTGCAACTCTATCGCACGAATATGGAGGCTGAGCACCAAAAGAAAATTTCAGATTCAAGTGGGAGTTCTGGGTCCAATGTGAAAAGGCTTGAAGAGGAAACTGATACAAAAATTAAGAACTTGAAAGAGTCAGCCTCAAAGGTCCAGTCGGATATTGTCGGCATGCTTATTAAGTATGTCACAACTGTGAAAACTTGA

Protein Analysis

111

Amino Acids

12.31

Weight (kDa)

8.92

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-ATPase_G PF03179 7 - 109 6.5e-31 Vacuolar (H+)-ATPase G subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 180
AdeI CACNNNGTG 1 cut(s) 70
AgsI TTSAA 3 cut(s) 192, 227, 259
AleI CACNNNNGTG 1 cut(s) 320
AluBI AGCT 2 cut(s) 113, 125
AluI AGCT 2 cut(s) 113, 125
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 1 cut(s) 100
ApoI RAATTY 1 cut(s) 180
ArsI GACNNNNNNTTYG 2 cut(s) 266, 298
AspS9I GGNCC 2 cut(s) 207, 277
AvaII GGWCC 2 cut(s) 207, 277
Bbv12I GWGCWC 1 cut(s) 171
BccI CCATC 1 cut(s) 88
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BcoDI GTCTC 1 cut(s) 100
BfaI CTAG 1 cut(s) 81
BlpI GCTNAGC 1 cut(s) 165
Bme18I GGWCC 2 cut(s) 207, 277
BmgT120I GGNCC 2 cut(s) 207, 277
BmiI GGNNCC 1 cut(s) 208
BmsI GCATC 2 cut(s) 36, 46
BplI GAGNNNNNCTC 2 cut(s) 254, 286
Bpu1102I GCTNAGC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 124
Bse1I ACTGG 1 cut(s) 280
BseGI GGATG 2 cut(s) 27, 61
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 1 cut(s) 280
BsiHKAI GWGCWC 1 cut(s) 171
BsmAI GTCTC 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 171
Bsp1720I GCTNAGC 1 cut(s) 165
BspCNI CTCAG 1 cut(s) 157
BspLI GGNNCC 1 cut(s) 208
BsrI ACTGG 1 cut(s) 280
Bst4CI ACNGT 1 cut(s) 322
Bst6I CTCTTC 1 cut(s) 222
BstC8I GCNNGC 2 cut(s) 100, 299
BstDEI CTNAG 1 cut(s) 165
BstF5I GGATG 2 cut(s) 27, 61
BstMAI GTCTC 1 cut(s) 100
BstNSI RCATGY 1 cut(s) 301
BtsCI GGATG 2 cut(s) 27, 61
Cac8I GCNNGC 2 cut(s) 100, 299
Cfr13I GGNCC 2 cut(s) 207, 277
CviAII CATG 1 cut(s) 298
CviJI RGCY 5 cut(s) 113, 125, 164, 223, 269
CviKI_1 RGCY 5 cut(s) 113, 125, 164, 223, 269
DdeI CTNAG 1 cut(s) 165
DraIII CACNNNGTG 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
Eco47I GGWCC 2 cut(s) 207, 277
FaeI CATG 1 cut(s) 301
FaiI YATR 3 cut(s) 158, 299, 312
FatI CATG 1 cut(s) 297
FokI GGATG 2 cut(s) 14, 68
FspBI CTAG 1 cut(s) 81
Hin1II CATG 1 cut(s) 301
HinfI GANTC 3 cut(s) 5, 188, 263
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 2 cut(s) 187, 286
Hpy188III TCNNGA 1 cut(s) 31
Hpy8I GTNNAC 1 cut(s) 73
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4V TGCA 1 cut(s) 139
HpyF3I CTNAG 1 cut(s) 165
Hsp92II CATG 1 cut(s) 301
LpnPI CCDG 4 cut(s) 38, 44, 189, 293
LweI GCATC 2 cut(s) 36, 46
MaeI CTAG 1 cut(s) 81
MaeIII GTNAC 1 cut(s) 313
MboII GAAGA 2 cut(s) 131, 239
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 2 cut(s) 180, 246
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 5 cut(s) 8, 17, 154, 223, 280
MseI TTAA 2 cut(s) 249, 306
MslI CAYNNNNRTG 3 cut(s) 32, 155, 320
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 1 cut(s) 208
NmuCI GTSAC 1 cut(s) 313
NspI RCATGY 1 cut(s) 301
OliI CACNNNNGTG 1 cut(s) 320
PaeI GCATGC 1 cut(s) 301
PfeI GAWTC 2 cut(s) 5, 188
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
PspN4I GGNNCC 1 cut(s) 208
PspPI GGNCC 2 cut(s) 207, 277
RseI CAYNNNNRTG 3 cut(s) 32, 155, 320
SaqAI TTAA 2 cut(s) 249, 306
Sau96I GGNCC 2 cut(s) 207, 277
SchI GAGTC 1 cut(s) 272
SduI GDGCHC 1 cut(s) 171
SetI ASST 3 cut(s) 115, 127, 279
SfaNI GCATC 2 cut(s) 36, 46
SinI GGWCC 2 cut(s) 207, 277
SmiMI CAYNNNNRTG 3 cut(s) 32, 155, 320
SmlI CTYRAG 1 cut(s) 103
SmoI CTYRAG 1 cut(s) 103
SphI GCATGC 1 cut(s) 301
Sse9I AATT 2 cut(s) 180, 246
SspMI CTAG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 322
TasI AATT 2 cut(s) 180, 246
TfiI GAWTC 2 cut(s) 5, 188
Tru1I TTAA 2 cut(s) 249, 306
Tru9I TTAA 2 cut(s) 249, 306
TseFI GTSAC 1 cut(s) 313
Tsp45I GTSAC 1 cut(s) 313
VpaK11BI GGWCC 2 cut(s) 207, 277
XapI RAATTY 1 cut(s) 180
XceI RCATGY 1 cut(s) 301
XspI CTAG 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.