RchiOBHm_Chr7g0228271

Catalytic subunit of the peripheral V1 complex of vacuolar ATPase (V-ATPase). V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008981
Physical Location & Seq
Reverse (-)
51611057 .. 51611750
694 bp
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UTR
Exon/CDS
Intron
PRQ20447

Sequence Viewer

Length: 240 bp
ATGGACTCCGTTAGAGGACAAGGAGGCATCCAGATGCTGCTAACAGCAGAAGAGGAAGCTCAACACATTGTTAACAGCGCTAGAAACTGTAAATGCACAAGTGGGAGTTCTGGATCCAATGTAAAAAGGCTCGAGGAAGAAACAGAAACGAAGATTAAGAGCTTGAAGGAATCGGCCTCCAAAGTCCAATCAGATATTGTTGGCATGGTTATGAAGTATATCACTGATGTGAAAATTTGA

Protein Analysis

79

Amino Acids

8.6

Weight (kDa)

6.56

Isoelectric Point (pI)

46.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-ATPase_G PF03179 33 - 78 6.9e-06 Vacuolar (H+)-ATPase G subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 108, 121
AcsI RAATTY 1 cut(s) 234
AfeI AGCGCT 1 cut(s) 79
AgsI TTSAA 1 cut(s) 166
AleI CACNNNNGTG 1 cut(s) 227
AluBI AGCT 2 cut(s) 59, 162
AluI AGCT 2 cut(s) 59, 162
AlwI GGATC 2 cut(s) 108, 121
AlwNI CAGNNNCTG 1 cut(s) 37
Ama87I CYCGRG 1 cut(s) 131
Aor51HI AGCGCT 1 cut(s) 79
AoxI GGCC 1 cut(s) 174
ApeKI GCWGC 1 cut(s) 37
ApoI RAATTY 1 cut(s) 234
AspLEI GCGC 1 cut(s) 80
AvaI CYCGRG 1 cut(s) 131
BamHI GGATCC 1 cut(s) 113
BbvI GCAGC 1 cut(s) 24
BfaI CTAG 1 cut(s) 81
BfoI RGCGCY 1 cut(s) 81
BisI GCNGC 1 cut(s) 38
BlsI GCNGC 1 cut(s) 39
BmeT110I CYCGRG 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 115
BmsI GCATC 2 cut(s) 24, 36
BseGI GGATG 1 cut(s) 27
BseXI GCAGC 1 cut(s) 24
BshFI GGCC 1 cut(s) 176
BsiHKCI CYCGRG 1 cut(s) 131
BsnI GGCC 1 cut(s) 176
BsoBI CYCGRG 1 cut(s) 131
Bsp143I GATC 1 cut(s) 113
BspANI GGCC 1 cut(s) 176
BspLI GGNNCC 1 cut(s) 115
BspPI GGATC 2 cut(s) 108, 121
BssMI GATC 1 cut(s) 113
Bst4CI ACNGT 1 cut(s) 89
Bst6I CTCTTC 1 cut(s) 45
BstF5I GGATG 1 cut(s) 27
BstH2I RGCGCY 1 cut(s) 81
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 1 cut(s) 116
BstMBI GATC 1 cut(s) 113
BstV1I GCAGC 1 cut(s) 24
BstX2I RGATCY 1 cut(s) 113
BstYI RGATCY 1 cut(s) 113
BsuRI GGCC 1 cut(s) 176
BtsCI GGATG 1 cut(s) 27
BtsIMutI CAGTG 1 cut(s) 222
CaiI CAGNNNCTG 1 cut(s) 37
CfoI GCGC 1 cut(s) 80
CviAII CATG 1 cut(s) 205
CviJI RGCY 4 cut(s) 59, 130, 162, 176
CviKI_1 RGCY 4 cut(s) 59, 130, 162, 176
DpnI GATC 1 cut(s) 115
DpnII GATC 1 cut(s) 113
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Eco47III AGCGCT 1 cut(s) 79
Eco88I CYCGRG 1 cut(s) 131
FaeI CATG 1 cut(s) 208
FaiI YATR 3 cut(s) 206, 212, 219
FatI CATG 1 cut(s) 204
Fnu4HI GCNGC 1 cut(s) 38
FokI GGATG 1 cut(s) 14
Fsp4HI GCNGC 1 cut(s) 38
FspBI CTAG 1 cut(s) 81
GlaI GCGC 1 cut(s) 79
GluI GCNGC 1 cut(s) 38
HaeII RGCGCY 1 cut(s) 81
HaeIII GGCC 1 cut(s) 176
HhaI GCGC 1 cut(s) 80
Hin1II CATG 1 cut(s) 208
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HinfI GANTC 2 cut(s) 5, 170
HpaI GTTAAC 1 cut(s) 73
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 1 cut(s) 193
Hpy188III TCNNGA 2 cut(s) 31, 111
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 1 cut(s) 89
HpyCH4V TGCA 1 cut(s) 96
Hsp92II CATG 1 cut(s) 208
HspAI GCGC 1 cut(s) 78
KspAI GTTAAC 1 cut(s) 73
Kzo9I GATC 1 cut(s) 113
LpnPI CCDG 2 cut(s) 44, 96
Lsp1109I GCAGC 1 cut(s) 24
LweI GCATC 2 cut(s) 24, 36
MaeI CTAG 1 cut(s) 81
MalI GATC 1 cut(s) 115
MboI GATC 1 cut(s) 113
MboII GAAGA 3 cut(s) 62, 149, 163
MflI RGATCY 1 cut(s) 113
MluCI AATT 1 cut(s) 234
MnlI CCTC 5 cut(s) 8, 17, 46, 127, 187
MseI TTAA 2 cut(s) 72, 156
MslI CAYNNNNRTG 3 cut(s) 32, 209, 227
NdeII GATC 1 cut(s) 113
NlaIII CATG 1 cut(s) 208
NlaIV GGNNCC 1 cut(s) 115
OliI CACNNNNGTG 1 cut(s) 227
PaeR7I CTCGAG 1 cut(s) 131
PfeI GAWTC 1 cut(s) 170
PkrI GCNGC 1 cut(s) 39
PspN4I GGNNCC 1 cut(s) 115
PspXI VCTCGAGB 1 cut(s) 131
PstNI CAGNNNCTG 1 cut(s) 37
PsuI RGATCY 1 cut(s) 113
RseI CAYNNNNRTG 3 cut(s) 32, 209, 227
SaqAI TTAA 2 cut(s) 72, 156
SatI GCNGC 1 cut(s) 38
Sau3AI GATC 1 cut(s) 113
SetI ASST 2 cut(s) 61, 164
SfaNI GCATC 2 cut(s) 24, 36
Sfr274I CTCGAG 1 cut(s) 131
SgeI CNNG 9 cut(s) 32, 43, 93, 111, 123, 143, 145, 175, 217
SlaI CTCGAG 1 cut(s) 131
SmiMI CAYNNNNRTG 3 cut(s) 32, 209, 227
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 1 cut(s) 234
SspMI CTAG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 89
TaqI TCGA 1 cut(s) 132
TasI AATT 1 cut(s) 234
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 2 cut(s) 72, 156
Tru9I TTAA 2 cut(s) 72, 156
TscAI CASTG 1 cut(s) 229
TseI GCWGC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 227
TspRI CASTG 1 cut(s) 229
XapI RAATTY 1 cut(s) 234
XhoI CTCGAG 1 cut(s) 131
XspI CTAG 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.