AT4G27510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006230
Physical Location & Seq
Reverse (-)
13746176 .. 13749131
2956 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27510.2

Sequence Viewer

Length: 2187 bp
ATGACGGCCAAGCGGAAGAGTAAGAAACGCCCAGCTTCGAAGGTCTCAGATCCCGTCAACTTATTCCGATCCCTTGCTTCAGCAATTACGTCCTCTCAGGTTTCTCAGAAGCCAATTTTGAAACGTTTATTGTGCCTTCTGGGTCCTTTATCATTAACCCAACCAACAAATTGGGAGAATTGTGACATTGCTTCACATCATTGGAATATCAAGTTCCGTGGAGAGAAAGTGGACTTGATCAATTTCGATGATGTCTGTTTCCTCTCTGATGTGTTGTTCACAGAGCTTGATAGGAGTCTCGAAAGCCTGTTCGCTACTTTATTCAAACAAAAGGCTGAAACTTGTCCTACATTTGAAAGTACAGAAGATAGTATTGAATTAGCTTCGCTGTTCTTAAAATGTTGTATGAAGATCATGTCTTTACTTGTGGCCAAACAAGAGCTTGTTCTGGAGAAGGCCAAAACTCTTCTCTCGATCCTCGGTAGATTGATTCGTGCAAGAAATGGAGATTGTTCTTTTGTATTCACGCATGACGGTTCTTTAGATCCCCGTCATACGTTTCTTTGTACAGGGATTGAGGTTTTTATGGATGAGATTTTGGTGAATAAGTCAATCAGCGACCTTCTTTTCGTAGTTGATTCTGCATTCTCCTCATGTAGACTGTTTAGTAAGCATGATAGGGCCGGTGTGGTGCAAATCGTTTCTGCTCATTTTATTATCGCAACTTCTGATGAGAAAACGAATCAAATGTGTGTTGAGAGATTATATTGGAAGAAAGGTAATGCATTTAGAACTCCCCAGATAAGCTTGAGTGCTGCTGTGTCCTTGCTGCTAAACCCGGTTATGTTCTCTGCACCAAAAATGATCCATGCCTATGTGGTTTTATTGGTGTCTGACGCCATTGGTATTTGTTCACATCCATGTTTTAAGGGATTAGACCTTCAGCTTATTGATCACTATATTGATGCATTTGAAAAGTCTGTTGTTTTGTATAAGAGACACATGTCTAAGAGTGAGAATGGCCTGTCTGGGAAGTTTGGCTTTTTGACGAGTAAGTCTCGGGTGGCTTTTGAACATCGTCTTTTGCCCTCAACACTGGCGAAAGTTAATGATGTTACACAGCAGCTAAAGGATTCATGGGATTCATATCAAAGTGATAATGCGAAGAGAGAAAACAATGAGCTGGTAGCGTATTCTGTTGCATACGCAAAGGAAAGCTTATGTGTATTTGATAGCTCCTGCTCTGAAAGTATGTTGTCTCAGACCTTATCAATTCTTGGTTGCGTGATACTTAGAGCTAGTTCTGATGATGTTATGGACTCTGTGTTGGAAAAATACAACACATCTAGTATGGAAGATCTATATCTGCTTGCATCCACACTTAAGTTCATGACTTGTTCGATGTTGCAAGCCATCCGGGTTCTCAGGAATTGGAACTGGCATGAAGCTGGAGGTGATGTTCGAGCTTGCAAGGAATACAAAGCCATGATGGATGTTGTTCAACGTTTTGAACAGTTCAATATTCATATGCCCTGTCAAAGTTTCCTGCGCGATAGAATGGAATCTTACCCTCACAGATCTAAGTGGATGCTTATGCATTTTTCAGGCTTACTGTCTGTAAGCTTTGCACTCAAGCTTGACTTTCTAGTGAAGGACTCCATTTTTGGAATGGTGATATCTATGTATTTATTCATCCTAGAAGGGGGTGACTTGGAAGCACTTGGGGATTCAGTTGGTCACTCAGAAAACACGTCATCGTCTATACTATCCAGTGGTTCCAAAAACTTGGCTGCTTCTGGTAAAGCTGACAAGACAGCAGTAGATAGGAAACAGAGCGGGGCAGTTGCTTTGAAGTTTCACAAAAACCGAACTTTGTACTTGGGGAAGGTGTCTGAAGCTAAAGGTCCAGAGAATGGTTCAGATTCAGGAGTTGGTGTTGAAGAGGAAACCTGCAATGGGGAGAGATTTCTGTGGTGTATGGCAGGAAAAGGTAACGTGACAAAAACCGATGTGGACGAGTTGGCAGATTTCATAGCGTGTGATCCCGGTAAGGACTACTCCGACTGGCTAAAAGGGAGAGAACGATTTCGGAACCAACGGTGGAAGTCAGACAAGATTGCTAAAGAAAGGTGGAATAAGAAGCAAAAAGCTTGGAGAGAGAACAGAGGAAGAGGGTCTAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

728

Amino Acids

81.81

Weight (kDa)

8.56

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7812 PF25104 130 - 574 1.7e-158 Domain of unknown function (DUF7812)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1054
Acc36I ACCTGC 1 cut(s) 1958
AccB7I CCANNNNNTGG 1 cut(s) 1913
AccBSI CCGCTC 1 cut(s) 1836
AccI GTMKAC 1 cut(s) 658
AccII CGCG 1 cut(s) 1551
AciI CCGC 2 cut(s) 13, 1836
AclI AACGTT 2 cut(s) 124, 1504
AclWI GGATC 6 cut(s) 44, 63, 469, 539, 859, 2036
AcoI YGGCCR 2 cut(s) 6, 429
AcuI CTGAAG 3 cut(s) 63, 926, 1914
AcyI GRCGYC 1 cut(s) 897
AfaI GTAC 3 cut(s) 361, 568, 1877
AfiI CCNNNNNNNGG 3 cut(s) 1702, 1913, 1956
AflII CTTAAG 1 cut(s) 1382
AflIII ACRYGT 2 cut(s) 1002, 1749
AjiI CACGTC 1 cut(s) 1752
AloI GAACNNNNNNTCC 2 cut(s) 1443, 1475
Alw26I GTCTC 5 cut(s) 49, 302, 991, 1062, 1263
AlwI GGATC 6 cut(s) 44, 63, 469, 539, 859, 2036
Ama87I CYCGRG 1 cut(s) 1059
AoxI GGCC 5 cut(s) 6, 429, 456, 681, 1021
ApeKI GCWGC 4 cut(s) 815, 829, 1123, 1790
ArsI GACNNNNNNTTYG 4 cut(s) 611, 643, 1646, 1678
Asp700I GAANNNNTTC 1 cut(s) 2085
AspLEI GCGC 1 cut(s) 1551
AspS9I GGNCC 3 cut(s) 143, 681, 1904
AsuC2I CCSGG 3 cut(s) 839, 1418, 2046
AsuHPI GGTGA 4 cut(s) 613, 1466, 1684, 1718
AsuII TTCGAA 1 cut(s) 38
AvaI CYCGRG 1 cut(s) 1059
AvaII GGWCC 2 cut(s) 143, 1904
BalI TGGCCA 1 cut(s) 431
BbvI GCAGC 4 cut(s) 802, 816, 1135, 1777
BccI CCATC 2 cut(s) 1421, 1483
BceAI ACGGC 1 cut(s) 21
BclI TGATCA 2 cut(s) 237, 952
BcnI CCSGG 3 cut(s) 839, 1418, 2046
BcoDI GTCTC 5 cut(s) 49, 302, 991, 1062, 1263
BfaI CTAG 4 cut(s) 1299, 1347, 1646, 1697
BfrI CTTAAG 1 cut(s) 1382
BfuAI ACCTGC 1 cut(s) 1958
BglII AGATCT 2 cut(s) 1357, 1577
BisI GCNGC 4 cut(s) 816, 830, 1124, 1791
BlsI GCNGC 4 cut(s) 817, 831, 1125, 1792
Bme1390I CCNGG 3 cut(s) 839, 1418, 2046
Bme18I GGWCC 2 cut(s) 143, 1904
BmeT110I CYCGRG 1 cut(s) 1059
BmgBI CACGTC 1 cut(s) 1752
BmgT120I GGNCC 3 cut(s) 143, 681, 1904
BmiI GGNNCC 3 cut(s) 144, 1777, 2093
BmrFI CCNGG 3 cut(s) 839, 1418, 2046
BmsI GCATC 3 cut(s) 955, 1382, 1578
BoxI GACNNNNGTC 1 cut(s) 1003
BplI GAGNNNNNCTC 2 cut(s) 1042, 1074
BpmI CTGGAG 2 cut(s) 470, 1470
Bpu14I TTCGAA 1 cut(s) 38
BpuEI CTTGAG 2 cut(s) 829, 1616
BpuMI CCSGG 3 cut(s) 839, 1418, 2046
BsaBI GATNNNNATC 2 cut(s) 1146, 1362
BsaHI GRCGYC 1 cut(s) 897
BsaI GGTCTC 1 cut(s) 49
BsaJI CCNNGG 2 cut(s) 217, 478
BsaXI ACNNNNNCTCC 6 cut(s) 213, 243, 1443, 1473, 1920, 1950
Bsc4I CCNNNNNNNGG 3 cut(s) 1702, 1913, 1956
Bse118I RCCGGY 1 cut(s) 683
Bse1I ACTGG 4 cut(s) 1101, 1442, 1770, 2069
Bse3DI GCAATG 2 cut(s) 186, 1960
Bse8I GATNNNNATC 2 cut(s) 1146, 1362
BseDI CCNNGG 2 cut(s) 217, 478
BseGI GGATG 7 cut(s) 595, 916, 1373, 1413, 1498, 1593, 1692
BseJI GATNNNNATC 2 cut(s) 1146, 1362
BseLI CCNNNNNNNGG 3 cut(s) 1702, 1913, 1956
BseMI GCAATG 2 cut(s) 186, 1960
BseMII CTCAG 6 cut(s) 60, 110, 119, 1274, 1438, 1755
BseNI ACTGG 4 cut(s) 1101, 1442, 1770, 2069
BseRI GAGGAG 1 cut(s) 640
BseXI GCAGC 4 cut(s) 802, 816, 1135, 1777
BseYI CCCAGC 1 cut(s) 31
BsgI GTGCAG 1 cut(s) 837
Bsh1236I CGCG 1 cut(s) 1551
BshFI GGCC 5 cut(s) 8, 431, 458, 683, 1023
BsiHKCI CYCGRG 1 cut(s) 1059
BsiSI CCGG 4 cut(s) 684, 839, 1417, 2046
BslI CCNNNNNNNGG 3 cut(s) 1702, 1913, 1956
BsmAI GTCTC 5 cut(s) 49, 302, 991, 1062, 1263
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 5 cut(s) 8, 431, 458, 683, 1023
Bso31I GGTCTC 1 cut(s) 49
BsoBI CYCGRG 1 cut(s) 1059
Bsp119I TTCGAA 1 cut(s) 38
Bsp1407I TGTACA 1 cut(s) 566
BspACI CCGC 2 cut(s) 13, 1836
BspANI GGCC 5 cut(s) 8, 431, 458, 683, 1023
BspCNI CTCAG 6 cut(s) 59, 109, 118, 1273, 1437, 1754
BspFNI CGCG 1 cut(s) 1551
BspHI TCATGA 1 cut(s) 1389
BspLI GGNNCC 3 cut(s) 144, 1777, 2093
BspMI ACCTGC 1 cut(s) 1958
BspPI GGATC 6 cut(s) 44, 63, 469, 539, 859, 2036
BspT104I TTCGAA 1 cut(s) 38
BspTI CTTAAG 1 cut(s) 1382
BspTNI GGTCTC 1 cut(s) 49
BsrBI CCGCTC 1 cut(s) 1836
BsrDI GCAATG 2 cut(s) 186, 1960
BsrFI RCCGGY 1 cut(s) 683
BsrGI TGTACA 1 cut(s) 566
BsrI ACTGG 4 cut(s) 1101, 1442, 1770, 2069
BssAI RCCGGY 1 cut(s) 683
BssECI CCNNGG 2 cut(s) 217, 478
BssNI GRCGYC 1 cut(s) 897
Bst4CI ACNGT 5 cut(s) 536, 663, 1515, 1614, 2100
Bst6I CTCTTC 5 cut(s) 11, 471, 1160, 1935, 2164
BstACI GRCGYC 1 cut(s) 897
BstAFI CTTAAG 1 cut(s) 1382
BstAUI TGTACA 1 cut(s) 566
BstBI TTCGAA 1 cut(s) 38
BstC8I GCNNGC 3 cut(s) 1371, 1410, 1468
BstDEI CTNAG 9 cut(s) 46, 96, 105, 1008, 1260, 1292, 1424, 1581, 1741
BstDSI CCRYGG 1 cut(s) 217
BstF5I GGATG 7 cut(s) 595, 916, 1373, 1413, 1498, 1593, 1692
BstFNI CGCG 1 cut(s) 1551
BstHHI GCGC 1 cut(s) 1551
BstMAI GTCTC 5 cut(s) 49, 302, 991, 1062, 1263
BstNSI RCATGY 1 cut(s) 1006
BstPAI GACNNNNGTC 1 cut(s) 1003
BstSCI CCNGG 3 cut(s) 837, 1416, 2044
BstUI CGCG 1 cut(s) 1551
BstV1I GCAGC 4 cut(s) 802, 816, 1135, 1777
BstX2I RGATCY 4 cut(s) 49, 544, 1357, 1577
BstXI CCANNNNNNTGG 2 cut(s) 171, 1786
BstYI RGATCY 4 cut(s) 49, 544, 1357, 1577
BsuRI GGCC 5 cut(s) 8, 431, 458, 683, 1023
BtgI CCRYGG 1 cut(s) 217
BtrI CACGTC 1 cut(s) 1752
BtsCI GGATG 7 cut(s) 595, 916, 1373, 1413, 1498, 1593, 1692
BtsIMutI CAGTG 2 cut(s) 1094, 1777
BveI ACCTGC 1 cut(s) 1958
Cac8I GCNNGC 3 cut(s) 1371, 1410, 1468
CciI TCATGA 1 cut(s) 1389
CfoI GCGC 1 cut(s) 1551
Cfr10I RCCGGY 1 cut(s) 683
Cfr13I GGNCC 3 cut(s) 143, 681, 1904
CseI GACGC 1 cut(s) 905
Csp6I GTAC 3 cut(s) 360, 567, 1876
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CviQI GTAC 3 cut(s) 360, 567, 1876
DdeI CTNAG 9 cut(s) 46, 96, 105, 1008, 1260, 1292, 1424, 1581, 1741
DrdI GACNNNNNNGTC 1 cut(s) 1054
DseDI GACNNNNNNGTC 1 cut(s) 1054
EaeI YGGCCR 2 cut(s) 6, 429
Eam1104I CTCTTC 5 cut(s) 11, 471, 1160, 1935, 2164
EarI CTCTTC 5 cut(s) 11, 471, 1160, 1935, 2164
Eco31I GGTCTC 1 cut(s) 49
Eco32I GATATC 1 cut(s) 1677
Eco47I GGWCC 2 cut(s) 143, 1904
Eco57I CTGAAG 3 cut(s) 63, 926, 1914
Eco88I CYCGRG 1 cut(s) 1059
EcoO109I RGGNCCY 1 cut(s) 143
EcoRV GATATC 1 cut(s) 1677
EcoT22I ATGCAT 3 cut(s) 787, 970, 1599
FalI AAGNNNNNCTT 6 cut(s) 1025, 1057, 1202, 1234, 1625, 1657
FauI CCCGC 1 cut(s) 1829
FauNDI CATATG 1 cut(s) 1527
FbaI TGATCA 2 cut(s) 237, 952
FblI GTMKAC 1 cut(s) 658
Fnu4HI GCNGC 4 cut(s) 816, 830, 1124, 1791
FokI GGATG 7 cut(s) 602, 903, 1360, 1400, 1505, 1600, 1679
Fsp4HI GCNGC 4 cut(s) 816, 830, 1124, 1791
FspBI CTAG 4 cut(s) 1299, 1347, 1646, 1697
GlaI GCGC 1 cut(s) 1550
GluI GCNGC 4 cut(s) 816, 830, 1124, 1791
GsaI CCCAGC 1 cut(s) 35
GsuI CTGGAG 2 cut(s) 470, 1470
HaeIII GGCC 5 cut(s) 8, 431, 458, 683, 1023
HapII CCGG 4 cut(s) 684, 839, 1417, 2046
HgaI GACGC 1 cut(s) 905
HhaI GCGC 1 cut(s) 1551
Hin1I GRCGYC 1 cut(s) 897
Hin6I GCGC 1 cut(s) 1549
HinP1I GCGC 1 cut(s) 1549
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HindIII AAGCTT 5 cut(s) 805, 1216, 1621, 1634, 2148
HpaII CCGG 4 cut(s) 684, 839, 1417, 2046
HphI GGTGA 4 cut(s) 613, 1466, 1684, 1718
Hpy166II GTNNAC 6 cut(s) 58, 232, 279, 659, 914, 2014
Hpy188III TCNNGA 7 cut(s) 299, 449, 472, 1390, 1426, 1907, 1926
Hpy8I GTNNAC 6 cut(s) 58, 232, 279, 659, 914, 2014
HpyAV CCTTC 8 cut(s) 34, 146, 448, 632, 950, 1645, 1694, 1879
HpyCH4III ACNGT 5 cut(s) 536, 663, 1515, 1614, 2100
HpyCH4IV ACGT 6 cut(s) 89, 124, 557, 1504, 1751, 1995
HpyF3I CTNAG 9 cut(s) 46, 96, 105, 1008, 1260, 1292, 1424, 1581, 1741
HpySE526I ACGT 6 cut(s) 89, 124, 557, 1504, 1751, 1995
Hsp92I GRCGYC 1 cut(s) 897
HspAI GCGC 1 cut(s) 1549
Ksp22I TGATCA 2 cut(s) 237, 952
LmnI GCTCC 1 cut(s) 1241
Lsp1109I GCAGC 4 cut(s) 802, 816, 1135, 1777
LweI GCATC 3 cut(s) 955, 1382, 1578
MaeI CTAG 4 cut(s) 1299, 1347, 1646, 1697
MaeII ACGT 6 cut(s) 89, 124, 557, 1504, 1751, 1995
MaeIII GTNAC 6 cut(s) 182, 1114, 1706, 1736, 1991, 1996
MbiI CCGCTC 1 cut(s) 1836
MboII GAAGA 9 cut(s) 28, 377, 421, 458, 784, 1177, 1367, 1952, 2181
MflI RGATCY 4 cut(s) 49, 544, 1357, 1577
MlsI TGGCCA 1 cut(s) 431
MluCI AATT 8 cut(s) 84, 114, 169, 178, 241, 377, 1272, 1429
MluNI TGGCCA 1 cut(s) 431
MlyI GAGTC 3 cut(s) 304, 1313, 1649
MmeI TCCRAC 2 cut(s) 1308, 2085
Mox20I TGGCCA 1 cut(s) 431
Mph1103I ATGCAT 3 cut(s) 787, 970, 1599
MroXI GAANNNNTTC 1 cut(s) 2085
MscI TGGCCA 1 cut(s) 431
MseI TTAA 5 cut(s) 155, 395, 927, 1107, 1383
MslI CAYNNNNRTG 2 cut(s) 873, 919
Msp20I TGGCCA 1 cut(s) 431
MspCI CTTAAG 1 cut(s) 1382
MspI CCGG 4 cut(s) 684, 839, 1417, 2046
MspR9I CCNGG 3 cut(s) 839, 1418, 2046
Mva1269I GAATGC 1 cut(s) 644
MvnI CGCG 1 cut(s) 1551
NciI CCSGG 3 cut(s) 839, 1418, 2046
NdeI CATATG 1 cut(s) 1527
NlaIV GGNNCC 3 cut(s) 144, 1777, 2093
NmuCI GTSAC 4 cut(s) 182, 1706, 1736, 1996
NsiI ATGCAT 3 cut(s) 787, 970, 1599
NspI RCATGY 1 cut(s) 1006
NspV TTCGAA 1 cut(s) 38
PagI TCATGA 1 cut(s) 1389
PciI ACATGT 1 cut(s) 1002
PctI GAATGC 1 cut(s) 644
PdmI GAANNNNTTC 1 cut(s) 2085
PfeI GAWTC 8 cut(s) 490, 638, 742, 1133, 1142, 1562, 1727, 1922
PflMI CCANNNNNTGG 1 cut(s) 1913
PkrI GCNGC 4 cut(s) 817, 831, 1125, 1792
PleI GAGTC 3 cut(s) 303, 1313, 1649
PpsI GAGTC 3 cut(s) 303, 1313, 1649
PpuMI RGGWCCY 1 cut(s) 143
PscI ACATGT 1 cut(s) 1002
PshAI GACNNNNGTC 1 cut(s) 1003
Psp1406I AACGTT 2 cut(s) 124, 1504
Psp5II RGGWCCY 1 cut(s) 143
PspFI CCCAGC 1 cut(s) 31
PspN4I GGNNCC 3 cut(s) 144, 1777, 2093
PspPI GGNCC 3 cut(s) 143, 681, 1904
PspPPI RGGWCCY 1 cut(s) 143
PsuI RGATCY 4 cut(s) 49, 544, 1357, 1577
RsaI GTAC 3 cut(s) 361, 568, 1877
RsaNI GTAC 3 cut(s) 360, 567, 1876
RseI CAYNNNNRTG 2 cut(s) 873, 919
SaqAI TTAA 5 cut(s) 155, 395, 927, 1107, 1383
SatI GCNGC 4 cut(s) 816, 830, 1124, 1791
Sau96I GGNCC 3 cut(s) 143, 681, 1904
SchI GAGTC 3 cut(s) 304, 1313, 1649
ScrFI CCNGG 3 cut(s) 839, 1418, 2046
SfaNI GCATC 3 cut(s) 955, 1382, 1578
SfuI TTCGAA 1 cut(s) 38
SinI GGWCC 2 cut(s) 143, 1904
SmiMI CAYNNNNRTG 2 cut(s) 873, 919
SmlI CTYRAG 3 cut(s) 808, 1382, 1631
SmoI CTYRAG 3 cut(s) 808, 1382, 1631
Sse9I AATT 8 cut(s) 84, 114, 169, 178, 241, 377, 1272, 1429
SsiI CCGC 2 cut(s) 13, 1836
SspI AATATT 1 cut(s) 1522
SspMI CTAG 4 cut(s) 1299, 1347, 1646, 1697
StyD4I CCNGG 3 cut(s) 837, 1416, 2044
TaaI ACNGT 5 cut(s) 536, 663, 1515, 1614, 2100
TaiI ACGT 6 cut(s) 92, 127, 560, 1507, 1754, 1998
TaqI TCGA 6 cut(s) 38, 246, 300, 473, 1400, 1462
TasI AATT 8 cut(s) 84, 114, 169, 178, 241, 377, 1272, 1429
TatI WGTACW 3 cut(s) 359, 566, 1875
TfiI GAWTC 8 cut(s) 490, 638, 742, 1133, 1142, 1562, 1727, 1922
Tru1I TTAA 5 cut(s) 155, 395, 927, 1107, 1383
Tru9I TTAA 5 cut(s) 155, 395, 927, 1107, 1383
TscAI CASTG 2 cut(s) 1101, 1777
TseFI GTSAC 4 cut(s) 182, 1706, 1736, 1996
TseI GCWGC 4 cut(s) 815, 829, 1123, 1790
Tsp45I GTSAC 4 cut(s) 182, 1706, 1736, 1996
TspDTI ATGAA 8 cut(s) 422, 1125, 1134, 1378, 1458, 1514, 1681, 2020
TspGWI ACGGA 1 cut(s) 206
TspRI CASTG 2 cut(s) 1101, 1777
Van91I CCANNNNNTGG 1 cut(s) 1913
Vha464I CTTAAG 1 cut(s) 1382
VpaK11BI GGWCC 2 cut(s) 143, 1904
XceI RCATGY 1 cut(s) 1006
XcmI CCANNNNNNNNNTGG 1 cut(s) 1666
XmiI GTMKAC 1 cut(s) 658
XmnI GAANNNNTTC 1 cut(s) 2085
XspI CTAG 4 cut(s) 1299, 1347, 1646, 1697
Zsp2I ATGCAT 3 cut(s) 787, 970, 1599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.