AT4G27940

Mitochondrial carrier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008269
Physical Location & Seq
Forward (+)
13904265 .. 13907405
3141 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27940.1

Sequence Viewer

Length: 1242 bp
ATGGTTGAGGCAGAGCGCGCGGAGAATAGTTGGATTGCTTCGGAAACGTCTCCGTCGTCGTCTCCGGTGGTAATAGAAAGCAGTGATTTTTCGATAACTGATGGAATGAGAGATGTGAGCGGATTTGGTATAGCTTCTCGACCTCAGTTGGACAAGGGTTTGTCGGAAAACAATATAGGGTTCACGGAAAGAGTTTTCTCTGCCGCCGGAGCTGCGGTTTTGTCCGCCGTTACTCTAAATCCTCTTGACGTTGTCAAGACTCGATTGCAAGCTCAAGCCGCCGGAATGTCGTACTCTCACCCACTCAGTAACTCCATTGGACGCATGGCATTTTTTGGCCCAAACATGATGTTTGCAGACTTGAGATGTTCTCCTTCATGTGCACGCGCTGGTGTTGAAGGTACTGTTTCTATTTGCCCACCGGATTGTTTCCAGTACAAAGGAACATTTGATGTCTTCACCAAGATCATACGACAGGAGGGTCTGGGACGCCTTTGGAGAGGGACTAATGCTGGTCTTGCCCTAGCTGTGCCCATGGTTGGGATATATCTCCCATTCTATGATATGTTCCGCAACCGGTTGGAAGAATTATCTCGGGAGAAAGCTCCTGCTATGACATTTTGTGTGCCTACTGTGGCAGGGTCTTTAGCGCGGTCCTTAGCTTGTACAGTCTGCTATCCTATTGATCTTGCAAGAACGCGTATGCAGGCATTCAAAGAAGCAAAAGCCGGTGTGAAGCCTCCGGGAGTTTTTAAAACTCTAGTTGGTGTTTTCTCTGAAGTCAGGACAGCAAATAACCTTGAAAGTAGCTTGCACAACTATCGTGGTCTCTGGAGAGGCTTGGGTGCACAGCTTGCCCGTGATGTTCCATTCTCAGCAATCTGCTGGTCGACCCTCGAGCCAATAAAAAAGAGGCTCCTTGGAGTTGCGGGCAATGATACAAATCTTGTTGGTGTTTTCGGTGCAACCTTTTCTGCTGGATTTATAGCTGGAAGTATCGCTGCTGCTGCTACTTGTCCTCTTGACGTTGCAAGAACAAGAAGACAGATAGAGAAGGACCCTGGTAGGGCATTGATGATGACGACACGACAGACACTAATAGAGGTTTGGAGGGATGGAGGGATGAGAGGACTGTTCATGGGAATGGGTCCACGAGTGGCACGTGCAGGACCATCGGTAGGGATAGTGGTTTCCTTCTACGAAGTGGTCAAGTATGTTCTGCATCGCCATGCTTCATCATGA

Protein Analysis

413

Amino Acids

44.53

Weight (kDa)

9.34

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 60 - 98 5.6e-06 Mitochondrial carrier protein
Mito_carr PF00153 147 - 195 1.1e-08 Mitochondrial carrier protein
Mito_carr PF00153 210 - 307 7.3e-13 Mitochondrial carrier protein
Mito_carr PF00153 320 - 410 8.4e-18 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 480
AccBSI CCGCTC 1 cut(s) 120
AccI GTMKAC 1 cut(s) 890
AccII CGCG 5 cut(s) 18, 20, 387, 652, 700
AciI CCGC 9 cut(s) 20, 120, 204, 215, 225, 279, 571, 652, 929
AcuI CTGAAG 1 cut(s) 798
AcvI CACGTG 1 cut(s) 1163
AcyI GRCGYC 1 cut(s) 490
AfaI GTAC 4 cut(s) 293, 403, 437, 667
AfiI CCNNNNNNNGG 4 cut(s) 539, 540, 1066, 1178
AflIII ACRYGT 1 cut(s) 698
AgeI ACCGGT 1 cut(s) 576
AgsI TTSAA 3 cut(s) 398, 715, 803
AjnI CCWGG 1 cut(s) 1060
AluBI AGCT 9 cut(s) 134, 212, 272, 527, 605, 662, 810, 853, 989
AluI AGCT 9 cut(s) 134, 212, 272, 527, 605, 662, 810, 853, 989
Alw21I GWGCWC 2 cut(s) 385, 850
Alw26I GTCTC 3 cut(s) 54, 66, 833
Alw44I GTGCAC 2 cut(s) 381, 846
Ama87I CYCGRG 2 cut(s) 594, 896
AoxI GGCC 1 cut(s) 337
ApaLI GTGCAC 2 cut(s) 381, 846
ApeKI GCWGC 4 cut(s) 212, 1001, 1004, 1007
AsiGI ACCGGT 1 cut(s) 576
AspLEI GCGC 4 cut(s) 18, 20, 389, 652
AspS9I GGNCC 5 cut(s) 338, 654, 1057, 1148, 1169
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 2 cut(s) 290, 451
AvaI CYCGRG 2 cut(s) 594, 896
AvaII GGWCC 4 cut(s) 654, 1057, 1148, 1169
BaeGI GKGCMC 3 cut(s) 385, 534, 850
BauI CACGAG 1 cut(s) 1152
BbrPI CACGTG 1 cut(s) 1163
BbsI GAAGAC 2 cut(s) 448, 1048
Bbv12I GWGCWC 2 cut(s) 385, 850
BbvI GCAGC 4 cut(s) 199, 988, 991, 994
BccI CCATC 3 cut(s) 95, 1109, 1180
BceAI ACGGC 1 cut(s) 212
BcgI CGANNNNNNTGC 4 cut(s) 803, 837, 1155, 1189
BciT130I CCWGG 1 cut(s) 1062
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 3 cut(s) 54, 66, 833
BfaI CTAG 2 cut(s) 524, 761
BisI GCNGC 6 cut(s) 204, 213, 279, 1002, 1005, 1008
BlsI GCNGC 6 cut(s) 205, 214, 280, 1003, 1006, 1009
Bme1390I CCNGG 2 cut(s) 744, 1062
Bme18I GGWCC 4 cut(s) 654, 1057, 1148, 1169
BmeT110I CYCGRG 2 cut(s) 594, 896
BmgT120I GGNCC 5 cut(s) 338, 654, 1057, 1148, 1169
BmiI GGNNCC 3 cut(s) 917, 1059, 1149
BmrFI CCNGG 2 cut(s) 744, 1062
BmsI GCATC 1 cut(s) 1231
BpiI GAAGAC 2 cut(s) 448, 1048
BplI GAGNNNNNCTC 2 cut(s) 355, 387
BpmI CTGGAG 1 cut(s) 853
Bpu10I CCTNAGC 1 cut(s) 658
BpuEI CTTGAG 2 cut(s) 258, 382
BpuMI CCSGG 1 cut(s) 744
BsaAI YACGTR 1 cut(s) 1163
BsaBI GATNNNNATC 1 cut(s) 942
BsaHI GRCGYC 1 cut(s) 490
BsaI GGTCTC 1 cut(s) 833
BsaJI CCNNGG 3 cut(s) 534, 919, 1060
BsaWI WCCGGW 3 cut(s) 64, 421, 576
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bsc4I CCNNNNNNNGG 4 cut(s) 539, 540, 1066, 1178
Bse118I RCCGGY 2 cut(s) 576, 728
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 1 cut(s) 940
Bse8I GATNNNNATC 1 cut(s) 942
BseBI CCWGG 1 cut(s) 1062
BseDI CCNNGG 3 cut(s) 534, 919, 1060
BseGI GGATG 2 cut(s) 1120, 1128
BseJI GATNNNNATC 1 cut(s) 942
BseLI CCNNNNNNNGG 4 cut(s) 539, 540, 1066, 1178
BseMI GCAATG 1 cut(s) 940
BseMII CTCAG 3 cut(s) 158, 319, 888
BseNI ACTGG 1 cut(s) 433
BsePI GCGCGC 1 cut(s) 16
BseSI GKGCMC 3 cut(s) 385, 534, 850
BseXI GCAGC 4 cut(s) 199, 988, 991, 994
BsgI GTGCAG 1 cut(s) 1185
Bsh1236I CGCG 5 cut(s) 18, 20, 387, 652, 700
BshFI GGCC 1 cut(s) 339
BshTI ACCGGT 1 cut(s) 576
BsiHKAI GWGCWC 2 cut(s) 385, 850
BsiHKCI CYCGRG 2 cut(s) 594, 896
BsiSI CCGG 7 cut(s) 65, 207, 282, 422, 577, 729, 743
BslFI GGGAC 2 cut(s) 501, 517
BslI CCNNNNNNNGG 4 cut(s) 539, 540, 1066, 1178
BsmAI GTCTC 3 cut(s) 54, 66, 833
BsmBI CGTCTC 2 cut(s) 54, 66
BsmFI GGGAC 2 cut(s) 501, 517
BsmI GAATGC 1 cut(s) 710
BsnI GGCC 1 cut(s) 339
Bso31I GGTCTC 1 cut(s) 833
BsoBI CYCGRG 2 cut(s) 594, 896
Bsp1286I GDGCHC 3 cut(s) 385, 534, 850
Bsp1407I TGTACA 1 cut(s) 665
Bsp143I GATC 2 cut(s) 465, 685
Bsp19I CCATGG 1 cut(s) 534
BspACI CCGC 9 cut(s) 20, 120, 204, 215, 225, 279, 571, 652, 929
BspANI GGCC 1 cut(s) 339
BspCNI CTCAG 3 cut(s) 157, 318, 887
BspFNI CGCG 5 cut(s) 18, 20, 387, 652, 700
BspHI TCATGA 1 cut(s) 1238
BspLI GGNNCC 3 cut(s) 917, 1059, 1149
BspTNI GGTCTC 1 cut(s) 833
BsrBI CCGCTC 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 940
BsrFI RCCGGY 2 cut(s) 576, 728
BsrGI TGTACA 1 cut(s) 665
BsrI ACTGG 1 cut(s) 433
BssAI RCCGGY 2 cut(s) 576, 728
BssECI CCNNGG 3 cut(s) 534, 919, 1060
BssHII GCGCGC 1 cut(s) 16
BssMI GATC 2 cut(s) 465, 685
BssNI GRCGYC 1 cut(s) 490
BssSI CACGAG 1 cut(s) 1152
BssT1I CCWWGG 2 cut(s) 534, 919
Bst2BI CACGAG 1 cut(s) 1152
Bst2UI CCWGG 1 cut(s) 1062
Bst4CI ACNGT 4 cut(s) 406, 634, 670, 1134
BstACI GRCGYC 1 cut(s) 490
BstAPI GCANNNNNTGC 1 cut(s) 854
BstAUI TGTACA 1 cut(s) 665
BstBAI YACGTR 1 cut(s) 1163
BstC8I GCNNGC 7 cut(s) 18, 270, 385, 708, 812, 855, 931
BstDEI CTNAG 4 cut(s) 144, 305, 658, 874
BstDSI CCRYGG 1 cut(s) 534
BstF5I GGATG 2 cut(s) 1120, 1128
BstFNI CGCG 5 cut(s) 18, 20, 387, 652, 700
BstHHI GCGC 4 cut(s) 18, 20, 389, 652
BstKTI GATC 2 cut(s) 468, 688
BstMAI GTCTC 3 cut(s) 54, 66, 833
BstMBI GATC 2 cut(s) 465, 685
BstMWI GCNNNNNNNGC 7 cut(s) 17, 209, 212, 278, 518, 854, 1007
BstNI CCWGG 1 cut(s) 1062
BstSCI CCNGG 2 cut(s) 742, 1060
BstSLI GKGCMC 3 cut(s) 385, 534, 850
BstUI CGCG 5 cut(s) 18, 20, 387, 652, 700
BstV1I GCAGC 4 cut(s) 199, 988, 991, 994
BstV2I GAAGAC 2 cut(s) 448, 1048
BsuRI GGCC 1 cut(s) 339
BtgI CCRYGG 1 cut(s) 534
BtgZI GCGATG 1 cut(s) 1208
BtsCI GGATG 2 cut(s) 1120, 1128
BtsI GCAGTG 1 cut(s) 88
BtsIMutI CAGTG 1 cut(s) 88
Cac8I GCNNGC 7 cut(s) 18, 270, 385, 708, 812, 855, 931
CciI TCATGA 1 cut(s) 1238
CfoI GCGC 4 cut(s) 18, 20, 389, 652
Cfr10I RCCGGY 2 cut(s) 576, 728
Cfr13I GGNCC 5 cut(s) 338, 654, 1057, 1148, 1169
CseI GACGC 2 cut(s) 330, 498
Csp6I GTAC 4 cut(s) 292, 402, 436, 666
CspAI ACCGGT 1 cut(s) 576
CspCI CAANNNNNGTGG 2 cut(s) 805, 840
CviAII CATG 7 cut(s) 325, 346, 378, 535, 1138, 1229, 1239
CviQI GTAC 4 cut(s) 292, 402, 436, 666
DdeI CTNAG 4 cut(s) 144, 305, 658, 874
DpnI GATC 2 cut(s) 467, 687
DpnII GATC 2 cut(s) 465, 685
DraI TTTAAA 1 cut(s) 754
DrdI GACNNNNNNGTC 1 cut(s) 480
DseDI GACNNNNNNGTC 1 cut(s) 480
EciI GGCGGA 1 cut(s) 214
Eco130I CCWWGG 2 cut(s) 534, 919
Eco31I GGTCTC 1 cut(s) 833
Eco47I GGWCC 4 cut(s) 654, 1057, 1148, 1169
Eco57I CTGAAG 1 cut(s) 798
Eco72I CACGTG 1 cut(s) 1163
Eco88I CYCGRG 2 cut(s) 594, 896
EcoO109I RGGNCCY 1 cut(s) 1057
EcoRII CCWGG 1 cut(s) 1060
EcoT14I CCWWGG 2 cut(s) 534, 919
ErhI CCWWGG 2 cut(s) 534, 919
Esp3I CGTCTC 2 cut(s) 54, 66
FaeI CATG 7 cut(s) 328, 349, 381, 538, 1141, 1232, 1242
FaqI GGGAC 2 cut(s) 501, 517
FatI CATG 7 cut(s) 324, 345, 377, 534, 1137, 1228, 1238
FauI CCCGC 1 cut(s) 922
FblI GTMKAC 1 cut(s) 890
Fnu4HI GCNGC 6 cut(s) 204, 213, 279, 1002, 1005, 1008
FokI GGATG 2 cut(s) 1127, 1135
Fsp4HI GCNGC 6 cut(s) 204, 213, 279, 1002, 1005, 1008
FspBI CTAG 2 cut(s) 524, 761
GlaI GCGC 4 cut(s) 17, 19, 388, 651
GluI GCNGC 6 cut(s) 204, 213, 279, 1002, 1005, 1008
GsuI CTGGAG 1 cut(s) 853
HaeIII GGCC 1 cut(s) 339
HapII CCGG 7 cut(s) 65, 207, 282, 422, 577, 729, 743
HgaI GACGC 2 cut(s) 330, 498
HhaI GCGC 4 cut(s) 18, 20, 389, 652
Hin1I GRCGYC 1 cut(s) 490
Hin1II CATG 7 cut(s) 328, 349, 381, 538, 1141, 1232, 1242
Hin6I GCGC 4 cut(s) 16, 18, 387, 650
HinP1I GCGC 4 cut(s) 16, 18, 387, 650
HincII GTYRAC 1 cut(s) 891
HindII GTYRAC 1 cut(s) 891
HinfI GANTC 1 cut(s) 259
HpaII CCGG 7 cut(s) 65, 207, 282, 422, 577, 729, 743
HphI GGTGA 2 cut(s) 290, 451
Hpy166II GTNNAC 5 cut(s) 183, 383, 848, 891, 1151
Hpy188I TCNGA 3 cut(s) 43, 166, 778
Hpy188III TCNNGA 8 cut(s) 138, 245, 256, 596, 784, 832, 1022, 1239
Hpy8I GTNNAC 5 cut(s) 183, 383, 848, 891, 1151
Hpy99I CGWCG 2 cut(s) 58, 61
HpyAV CCTTC 4 cut(s) 384, 392, 1048, 1204
HpyCH4III ACNGT 4 cut(s) 406, 634, 670, 1134
HpyCH4IV ACGT 4 cut(s) 47, 249, 1026, 1162
HpyF10VI GCNNNNNNNGC 7 cut(s) 17, 209, 212, 278, 518, 854, 1007
HpyF3I CTNAG 4 cut(s) 144, 305, 658, 874
HpySE526I ACGT 4 cut(s) 47, 249, 1026, 1162
Hsp92I GRCGYC 1 cut(s) 490
Hsp92II CATG 7 cut(s) 328, 349, 381, 538, 1141, 1232, 1242
HspAI GCGC 4 cut(s) 16, 18, 387, 650
Kzo9I GATC 2 cut(s) 465, 685
LmnI GCTCC 3 cut(s) 209, 610, 921
Lsp1109I GCAGC 4 cut(s) 199, 988, 991, 994
LweI GCATC 1 cut(s) 1231
MaeI CTAG 2 cut(s) 524, 761
MaeII ACGT 4 cut(s) 47, 249, 1026, 1162
MaeIII GTNAC 2 cut(s) 229, 308
MalI GATC 2 cut(s) 467, 687
MbiI CCGCTC 1 cut(s) 120
MboI GATC 2 cut(s) 465, 685
MboII GAAGA 3 cut(s) 448, 596, 1053
MhlI GDGCHC 3 cut(s) 385, 534, 850
MluCI AATT 1 cut(s) 587
MluI ACGCGT 1 cut(s) 698
MlyI GAGTC 1 cut(s) 253
MmeI TCCRAC 4 cut(s) 11, 129, 144, 561
MseI TTAA 1 cut(s) 753
MslI CAYNNNNRTG 2 cut(s) 1142, 1227
MspI CCGG 7 cut(s) 65, 207, 282, 422, 577, 729, 743
MspR9I CCNGG 2 cut(s) 744, 1062
Mva1269I GAATGC 1 cut(s) 710
MvaI CCWGG 1 cut(s) 1062
MvnI CGCG 5 cut(s) 18, 20, 387, 652, 700
MwoI GCNNNNNNNGC 7 cut(s) 17, 209, 212, 278, 518, 854, 1007
NciI CCSGG 1 cut(s) 744
NcoI CCATGG 1 cut(s) 534
NdeII GATC 2 cut(s) 465, 685
NlaIII CATG 7 cut(s) 328, 349, 381, 538, 1141, 1232, 1242
NlaIV GGNNCC 3 cut(s) 917, 1059, 1149
PaeR7I CTCGAG 1 cut(s) 896
PagI TCATGA 1 cut(s) 1238
PauI GCGCGC 1 cut(s) 16
PcsI WCGNNNNNNNCGW 2 cut(s) 53, 1159
PctI GAATGC 1 cut(s) 710
PflFI GACNNNGTC 1 cut(s) 251
PfoI TCCNGGA 1 cut(s) 742
PinAI ACCGGT 1 cut(s) 576
PkrI GCNGC 6 cut(s) 205, 214, 280, 1003, 1006, 1009
PleI GAGTC 1 cut(s) 253
PmaCI CACGTG 1 cut(s) 1163
PmlI CACGTG 1 cut(s) 1163
PpsI GAGTC 1 cut(s) 253
Ppu21I YACGTR 1 cut(s) 1163
PpuMI RGGWCCY 1 cut(s) 1057
Psp5II RGGWCCY 1 cut(s) 1057
Psp6I CCWGG 1 cut(s) 1060
PspCI CACGTG 1 cut(s) 1163
PspGI CCWGG 1 cut(s) 1060
PspN4I GGNNCC 3 cut(s) 917, 1059, 1149
PspPI GGNCC 5 cut(s) 338, 654, 1057, 1148, 1169
PspPPI RGGWCCY 1 cut(s) 1057
PspXI VCTCGAGB 1 cut(s) 896
PsyI GACNNNGTC 1 cut(s) 251
PteI GCGCGC 1 cut(s) 16
RsaI GTAC 4 cut(s) 293, 403, 437, 667
RsaNI GTAC 4 cut(s) 292, 402, 436, 666
RseI CAYNNNNRTG 2 cut(s) 1142, 1227
SalI GTCGAC 1 cut(s) 889
SaqAI TTAA 1 cut(s) 753
SatI GCNGC 6 cut(s) 204, 213, 279, 1002, 1005, 1008
Sau3AI GATC 2 cut(s) 465, 685
Sau96I GGNCC 5 cut(s) 338, 654, 1057, 1148, 1169
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 2 cut(s) 744, 1062
SduI GDGCHC 3 cut(s) 385, 534, 850
SfaNI GCATC 1 cut(s) 1231
Sfr274I CTCGAG 1 cut(s) 896
SinI GGWCC 4 cut(s) 654, 1057, 1148, 1169
SlaI CTCGAG 1 cut(s) 896
SmiMI CAYNNNNRTG 2 cut(s) 1142, 1227
SmlI CTYRAG 3 cut(s) 273, 361, 896
SmoI CTYRAG 3 cut(s) 273, 361, 896
Sse9I AATT 1 cut(s) 587
SsiI CCGC 9 cut(s) 20, 120, 204, 215, 225, 279, 571, 652, 929
SspMI CTAG 2 cut(s) 524, 761
StyD4I CCNGG 2 cut(s) 742, 1060
StyI CCWWGG 2 cut(s) 534, 919
TaaI ACNGT 4 cut(s) 406, 634, 670, 1134
TaiI ACGT 4 cut(s) 50, 252, 1029, 1165
TaqI TCGA 5 cut(s) 92, 139, 262, 890, 897
TasI AATT 1 cut(s) 587
TatI WGTACW 2 cut(s) 435, 665
TauI GCSGC 2 cut(s) 206, 281
Tru1I TTAA 1 cut(s) 753
Tru9I TTAA 1 cut(s) 753
TscAI CASTG 1 cut(s) 88
TseI GCWGC 4 cut(s) 212, 1001, 1004, 1007
TspDTI ATGAA 3 cut(s) 366, 1126, 1224
TspGWI ACGGA 2 cut(s) 42, 200
TspRI CASTG 1 cut(s) 88
Tth111I GACNNNGTC 1 cut(s) 251
VneI GTGCAC 2 cut(s) 381, 846
VpaK11BI GGWCC 4 cut(s) 654, 1057, 1148, 1169
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
XhoI CTCGAG 1 cut(s) 896
XmiI GTMKAC 1 cut(s) 890
XspI CTAG 2 cut(s) 524, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.