Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0008269

Physical Location & Seq

Genomic Coordinates Forward (+)

51951702 .. 51960098

Gene Structure 8397 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0417100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1068 bp

ATGGGGGATCAAGACGAGAGCCGTTCGAGCTCATTTCCGATGGGGAAGACTGCACAAGAGAGGGGCTTGCCCTACGTGCCCGAGTGTTATGTGATTCCAACTTCACATCGTGCCAGCTTAACTCCAGAAGTTGCCAATGTACCCACCATCGATTTTGGTAAGCTCAAACAGAGTTCTGATGAAGAACGAGCGACTGTAATCCAAGAAATTAGAACCGCCTGTCGTCAGCTTGGATTCTTTAAAATTATCAACCATGGAATCCATCAATCGGTGCTAGATAGGGCGCTGGCTATGGCTTTGGAGTTTTTCGAGATGCCTGGTTCAGAGAAGGCAAAGTTCATGTCTAACGATGTTCACAAACCAGTGAGGTATGGGACAAGCTTCAAAGATGGAGTTGATAAGATTCAATTCTGGAGGGTTTTCCTCAAACATTATGCTCACCCCTTGGAAAATTGGATCGATTCTTGGCCAAACAATCCACCAAGCTACAGGGAAACCATGGGAGAGTACTGCAGAGAAGTAATGAAACTGAGCTTGGAAATAACAAGTGCCATCACCGAGAGCCTAGGCATAGGTCCAACCTACTTAACCAAGAAAATGGAAAACGGAATGCAAGTGATTACAGTCAATGGCTACCCACCGTGCCCCAGGCCAGAAATGGCGCTAGGGTTGCCGCCTCATTCGGACTACAGCTGCTTCACCATTGTGCTTCAGAGTTTCCCTGGACTGGAAATCATGCATGCTGAGGATGGAAAATGGAAACTAATCCCGCAACTTGAGAGTACTGCTCTTCAAGTCCATGTGGGTGACCATTTTGAGGTACTAAGCAATGGGTTGTACAAGAGTGTTGTGCACAGGGCGACACTGAACAGCGACAGGACTAGAATCTCCATTGCTAGCTTACACAGTTTGGGACTCGATGAGAAGATGAAGACTGCCGAGGAGCTTGTGAATGAAGAACAGCCTGCGAGGTACAGAGAAAGTAGCTTCAGAGATTTTCTGAATTTTCTCTCAAGCAATGATATAGCAGACGGGACCAGCTTCCTCAACTCACTCAAACTAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006810 GO:0006811 GO:0006812 GO:0006828 GO:0006839 GO:0006950 GO:0008150 GO:0009635 GO:0009636 GO:0010033 GO:0016020 GO:0016021 GO:0016530 GO:0019866 GO:0022857 GO:0030001 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031975 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0140104 GO:1901562

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mito_carr

Protein Analysis

355

Amino Acids

40.05

Weight (kDa)

5.87

Isoelectric Point (pI)

41.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	47 - 158	2.7e-24	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	206 - 301	1.7e-22	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008269)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27940
fragaria_vesca	FvH4_7g30980 FvH4_7g30980
malus_domestica	MD01G1214800.v1.1 MD07G1284500.v1.1
prunus_persica	Prupe.2G306700_v2.0.a1 Prupe.2G306700_v2.0.a1 Prupe.2G306700_v2.0.a1
pyrus_communis	pycom01g22560 pycom07g25840
rosa_chinensis	RchiOBHm_Chr1g0380061
rosa_laevigata	RLG00000026335
rosa_multiflora	Rmu_sc0001232.1_g000004 Rmu_sc0004184.1_g000004
rosa_roxburghii	Rroxscaffold_4G00279750
rosa_rugosa	Rorug01G0417000 Rorug01G0417100 Rorug01G0417200
rosa_samantha	Rh1AG441600 Rh1BG397200 Rh1CG410500 Rh1DG428000
rosa_wichuraiana	Rw1G038390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	3 cut(s)	216, 674, 770
AclWI	GGATC	2 cut(s)	15, 464
AcoI	YGGCCR	1 cut(s)	467
AcsI	RAATTY	1 cut(s)	1003
AcuI	CTGAAG	2 cut(s)	695, 973
AdeI	CACNNNGTG	1 cut(s)	110
AfaI	GTAC	6 cut(s)	141, 509, 784, 822, 839, 974
AfiI	CCNNNNNNNGG	3 cut(s)	268, 727, 817
AgsI	TTSAA	3 cut(s)	385, 407, 794
AjnI	CCWGG	3 cut(s)	316, 647, 721
AjuI	GAANNNNNNNTTGG	2 cut(s)	518, 550
AleI	CACNNNNGTG	1 cut(s)	704
Alw21I	GWGCWC	2 cut(s)	32, 855
Alw44I	GTGCAC	1 cut(s)	851
AlwI	GGATC	2 cut(s)	15, 464
Ama87I	CYCGRG	1 cut(s)	80
AoxI	GGCC	2 cut(s)	467, 650
ApaLI	GTGCAC	1 cut(s)	851
ApeKI	GCWGC	1 cut(s)	693
ApoI	RAATTY	1 cut(s)	1003
ArsI	GACNNNNNNTTYG	2 cut(s)	326, 358
AspA2I	CCTAGG	1 cut(s)	565
AspLEI	GCGC	2 cut(s)	286, 664
AspS9I	GGNCC	2 cut(s)	575, 1035
AsuHPI	GGTGA	4 cut(s)	431, 547, 691, 818
AsuNHI	GCTAGC	1 cut(s)	896
AvaI	CYCGRG	1 cut(s)	80
AvaII	GGWCC	2 cut(s)	575, 1035
AvrII	CCTAGG	1 cut(s)	565
BaeGI	GKGCMC	3 cut(s)	81, 647, 855
BalI	TGGCCA	1 cut(s)	469
BanII	GRGCYC	1 cut(s)	32
BbsI	GAAGAC	2 cut(s)	53, 938
Bbv12I	GWGCWC	2 cut(s)	32, 855
BbvCI	CCTCAGC	1 cut(s)	744
BbvI	GCAGC	1 cut(s)	680
BccI	CCATC	6 cut(s)	34, 155, 270, 383, 560, 743
BceAI	ACGGC	1 cut(s)	6
BciT130I	CCWGG	3 cut(s)	318, 649, 723
BfaI	CTAG	6 cut(s)	275, 566, 665, 882, 897, 1061
BfmI	CTRYAG	3 cut(s)	487, 511, 688
BfoI	RGCGCY	2 cut(s)	287, 665
BisI	GCNGC	2 cut(s)	674, 694
BlnI	CCTAGG	1 cut(s)	565
BlsI	GCNGC	2 cut(s)	675, 695
BmcAI	AGTACT	2 cut(s)	509, 784
Bme1390I	CCNGG	3 cut(s)	318, 649, 723
Bme18I	GGWCC	2 cut(s)	575, 1035
BmeT110I	CYCGRG	1 cut(s)	80
BmgT120I	GGNCC	2 cut(s)	575, 1035
BmiI	GGNNCC	1 cut(s)	1036
BmrFI	CCNGG	3 cut(s)	318, 649, 723
BmsI	GCATC	1 cut(s)	303
BmtI	GCTAGC	1 cut(s)	900
BpiI	GAAGAC	2 cut(s)	53, 938
BplI	GAGNNNNNCTC	2 cut(s)	772, 804
BpmI	CTGGAG	2 cut(s)	108, 433
Bpu10I	CCTNAGC	1 cut(s)	744
BpuEI	CTTGAG	2 cut(s)	797, 997
Bsa29I	ATCGAT	2 cut(s)	150, 459
BsaAI	YACGTR	1 cut(s)	76
BsaJI	CCNNGG	7 cut(s)	253, 444, 498, 565, 647, 721, 939
Bsc4I	CCNNNNNNNGG	3 cut(s)	268, 727, 817
Bse1I	ACTGG	2 cut(s)	362, 732
Bse3DI	GCAATG	3 cut(s)	835, 891, 1024
BseBI	CCWGG	3 cut(s)	318, 649, 723
BseCI	ATCGAT	2 cut(s)	150, 459
BseDI	CCNNGG	7 cut(s)	253, 444, 498, 565, 647, 721, 939
BseGI	GGATG	1 cut(s)	754
BseLI	CCNNNNNNNGG	3 cut(s)	268, 727, 817
BseMI	GCAATG	3 cut(s)	835, 891, 1024
BseMII	CTCAG	2 cut(s)	521, 735
BseNI	ACTGG	2 cut(s)	362, 732
BseRI	GAGGAG	1 cut(s)	956
BseSI	GKGCMC	3 cut(s)	81, 647, 855
BseXI	GCAGC	1 cut(s)	680
BsgI	GTGCAG	1 cut(s)	36
BshFI	GGCC	2 cut(s)	469, 652
BshVI	ATCGAT	2 cut(s)	150, 459
BsiHKAI	GWGCWC	2 cut(s)	32, 855
BsiHKCI	CYCGRG	1 cut(s)	80
BslFI	GGGAC	3 cut(s)	388, 927, 1048
BslI	CCNNNNNNNGG	3 cut(s)	268, 727, 817
BsmFI	GGGAC	3 cut(s)	388, 927, 1048
BsmI	GAATGC	1 cut(s)	615
BsnI	GGCC	2 cut(s)	469, 652
BsoBI	CYCGRG	1 cut(s)	80
Bsp1286I	GDGCHC	4 cut(s)	32, 81, 647, 855
Bsp1407I	TGTACA	1 cut(s)	837
Bsp143I	GATC	2 cut(s)	7, 456
Bsp19I	CCATGG	2 cut(s)	253, 498
BspACI	CCGC	3 cut(s)	216, 674, 770
BspANI	GGCC	2 cut(s)	469, 652
BspCNI	CTCAG	2 cut(s)	522, 736
BspDI	ATCGAT	2 cut(s)	150, 459
BspLI	GGNNCC	1 cut(s)	1036
BspMAI	CTGCAG	1 cut(s)	515
BspOI	GCTAGC	1 cut(s)	900
BspPI	GGATC	2 cut(s)	15, 464
BspQI	GCTCTTC	1 cut(s)	795
BsrDI	GCAATG	3 cut(s)	835, 891, 1024
BsrGI	TGTACA	1 cut(s)	837
BsrI	ACTGG	2 cut(s)	362, 732
BssECI	CCNNGG	7 cut(s)	253, 444, 498, 565, 647, 721, 939
BssMI	GATC	2 cut(s)	7, 456
BssT1I	CCWWGG	4 cut(s)	253, 444, 498, 565
Bst2UI	CCWGG	3 cut(s)	318, 649, 723
Bst4CI	ACNGT	4 cut(s)	196, 625, 642, 908
Bst6I	CTCTTC	1 cut(s)	795
BstAUI	TGTACA	1 cut(s)	837
BstBAI	YACGTR	1 cut(s)	76
BstC8I	GCNNGC	6 cut(s)	68, 115, 288, 741, 898, 966
BstDEI	CTNAG	3 cut(s)	530, 744, 824
BstDSI	CCRYGG	2 cut(s)	253, 498
BstEII	GGTNACC	1 cut(s)	806
BstF5I	GGATG	1 cut(s)	754
BstH2I	RGCGCY	2 cut(s)	287, 665
BstHHI	GCGC	2 cut(s)	286, 664
BstKTI	GATC	2 cut(s)	10, 459
BstMBI	GATC	2 cut(s)	7, 456
BstMWI	GCNNNNNNNGC	3 cut(s)	27, 76, 670
BstNI	CCWGG	3 cut(s)	318, 649, 723
BstNSI	RCATGY	1 cut(s)	743
BstPI	GGTNACC	1 cut(s)	806
BstSCI	CCNGG	3 cut(s)	316, 647, 721
BstSFI	CTRYAG	3 cut(s)	487, 511, 688
BstSLI	GKGCMC	3 cut(s)	81, 647, 855
BstV1I	GCAGC	1 cut(s)	680
BstV2I	GAAGAC	2 cut(s)	53, 938
BstXI	CCANNNNNNTGG	1 cut(s)	598
Bsu15I	ATCGAT	2 cut(s)	150, 459
BsuRI	GGCC	2 cut(s)	469, 652
BsuTUI	ATCGAT	2 cut(s)	150, 459
BtgI	CCRYGG	2 cut(s)	253, 498
BtsCI	GGATG	1 cut(s)	754
BtsIMutI	CAGTG	2 cut(s)	369, 863
Cac8I	GCNNGC	6 cut(s)	68, 115, 288, 741, 898, 966
CfoI	GCGC	2 cut(s)	286, 664
Cfr13I	GGNCC	2 cut(s)	575, 1035
ClaI	ATCGAT	2 cut(s)	150, 459
Csp6I	GTAC	6 cut(s)	140, 508, 783, 821, 838, 973
CviAII	CATG	6 cut(s)	254, 340, 499, 736, 740, 800
CviQI	GTAC	6 cut(s)	140, 508, 783, 821, 838, 973
DdeI	CTNAG	3 cut(s)	530, 744, 824
DpnI	GATC	2 cut(s)	9, 458
DpnII	GATC	2 cut(s)	7, 456
DraI	TTTAAA	1 cut(s)	241
DraIII	CACNNNGTG	1 cut(s)	110
EaeI	YGGCCR	1 cut(s)	467
Eam1104I	CTCTTC	1 cut(s)	795
EarI	CTCTTC	1 cut(s)	795
Ecl136II	GAGCTC	1 cut(s)	30
Eco130I	CCWWGG	4 cut(s)	253, 444, 498, 565
Eco24I	GRGCYC	1 cut(s)	32
Eco47I	GGWCC	2 cut(s)	575, 1035
Eco53kI	GAGCTC	1 cut(s)	30
Eco57I	CTGAAG	2 cut(s)	695, 973
Eco88I	CYCGRG	1 cut(s)	80
Eco91I	GGTNACC	1 cut(s)	806
EcoICRI	GAGCTC	1 cut(s)	30
EcoO65I	GGTNACC	1 cut(s)	806
EcoRII	CCWGG	3 cut(s)	316, 647, 721
EcoT14I	CCWWGG	4 cut(s)	253, 444, 498, 565
EcoT22I	ATGCAT	1 cut(s)	741
EcoT38I	GRGCYC	1 cut(s)	32
ErhI	CCWWGG	4 cut(s)	253, 444, 498, 565
FaeI	CATG	6 cut(s)	257, 343, 502, 739, 743, 803
FaqI	GGGAC	3 cut(s)	388, 927, 1048
FatI	CATG	6 cut(s)	253, 339, 498, 735, 739, 799
FauI	CCCGC	1 cut(s)	777
Fnu4HI	GCNGC	2 cut(s)	674, 694
FokI	GGATG	1 cut(s)	761
FriOI	GRGCYC	1 cut(s)	32
Fsp4HI	GCNGC	2 cut(s)	674, 694
FspBI	CTAG	6 cut(s)	275, 566, 665, 882, 897, 1061
GlaI	GCGC	2 cut(s)	285, 663
GluI	GCNGC	2 cut(s)	674, 694
GsuI	CTGGAG	2 cut(s)	108, 433
HaeII	RGCGCY	2 cut(s)	287, 665
HaeIII	GGCC	2 cut(s)	469, 652
HhaI	GCGC	2 cut(s)	286, 664
Hin1II	CATG	6 cut(s)	257, 343, 502, 739, 743, 803
Hin6I	GCGC	2 cut(s)	284, 662
HinP1I	GCGC	2 cut(s)	284, 662
HindIII	AAGCTT	1 cut(s)	379
HinfI	GANTC	7 cut(s)	94, 234, 258, 403, 461, 885, 915
HphI	GGTGA	4 cut(s)	431, 547, 691, 818
Hpy166II	GTNNAC	2 cut(s)	355, 853
Hpy188I	TCNGA	7 cut(s)	39, 178, 325, 685, 714, 992, 1002
Hpy188III	TCNNGA	4 cut(s)	11, 125, 310, 412
Hpy8I	GTNNAC	2 cut(s)	355, 853
HpyAV	CCTTC	1 cut(s)	322
HpyCH4III	ACNGT	4 cut(s)	196, 625, 642, 908
HpyCH4IV	ACGT	1 cut(s)	75
HpyCH4V	TGCA	5 cut(s)	53, 513, 613, 739, 853
HpyF10VI	GCNNNNNNNGC	3 cut(s)	27, 76, 670
HpyF3I	CTNAG	3 cut(s)	530, 744, 824
HpySE526I	ACGT	1 cut(s)	75
Hsp92II	CATG	6 cut(s)	257, 343, 502, 739, 743, 803
HspAI	GCGC	2 cut(s)	284, 662
Kzo9I	GATC	2 cut(s)	7, 456
LguI	GCTCTTC	1 cut(s)	795
LmnI	GCTCC	1 cut(s)	943
Lsp1109I	GCAGC	1 cut(s)	680
LweI	GCATC	1 cut(s)	303
MaeI	CTAG	6 cut(s)	275, 566, 665, 882, 897, 1061
MaeII	ACGT	1 cut(s)	75
MaeIII	GTNAC	1 cut(s)	806
MalI	GATC	2 cut(s)	9, 458
MboI	GATC	2 cut(s)	7, 456
MboII	GAAGA	6 cut(s)	58, 194, 782, 937, 943, 968
MhlI	GDGCHC	4 cut(s)	32, 81, 647, 855
MlsI	TGGCCA	1 cut(s)	469
MluCI	AATT	5 cut(s)	207, 243, 407, 451, 1003
MluNI	TGGCCA	1 cut(s)	469
MlyI	GAGTC	1 cut(s)	909
MmeI	TCCRAC	2 cut(s)	122, 602
Mox20I	TGGCCA	1 cut(s)	469
Mph1103I	ATGCAT	1 cut(s)	741
MscI	TGGCCA	1 cut(s)	469
MseI	TTAA	3 cut(s)	119, 240, 587
MslI	CAYNNNNRTG	2 cut(s)	704, 804
Msp20I	TGGCCA	1 cut(s)	469
MspA1I	CMGCKG	1 cut(s)	693
MspR9I	CCNGG	3 cut(s)	318, 649, 723
Mva1269I	GAATGC	1 cut(s)	615
MvaI	CCWGG	3 cut(s)	318, 649, 723
MwoI	GCNNNNNNNGC	3 cut(s)	27, 76, 670
NcoI	CCATGG	2 cut(s)	253, 498
NdeII	GATC	2 cut(s)	7, 456
NheI	GCTAGC	1 cut(s)	896
NlaIII	CATG	6 cut(s)	257, 343, 502, 739, 743, 803
NlaIV	GGNNCC	1 cut(s)	1036
NmeAIII	GCCGAG	1 cut(s)	964
NmuCI	GTSAC	1 cut(s)	806
NsiI	ATGCAT	1 cut(s)	741
NspI	RCATGY	1 cut(s)	743
OliI	CACNNNNGTG	1 cut(s)	704
PaeI	GCATGC	1 cut(s)	743
PciSI	GCTCTTC	1 cut(s)	795
PctI	GAATGC	1 cut(s)	615
PfeI	GAWTC	6 cut(s)	94, 234, 258, 403, 461, 885
PkrI	GCNGC	2 cut(s)	675, 695
PleI	GAGTC	1 cut(s)	909
PpsI	GAGTC	1 cut(s)	909
Ppu21I	YACGTR	1 cut(s)	76
Psp124BI	GAGCTC	1 cut(s)	32
Psp6I	CCWGG	3 cut(s)	316, 647, 721
PspEI	GGTNACC	1 cut(s)	806
PspGI	CCWGG	3 cut(s)	316, 647, 721
PspN4I	GGNNCC	1 cut(s)	1036
PspPI	GGNCC	2 cut(s)	575, 1035
PstI	CTGCAG	1 cut(s)	515
PvuII	CAGCTG	1 cut(s)	693
RsaI	GTAC	6 cut(s)	141, 509, 784, 822, 839, 974
RsaNI	GTAC	6 cut(s)	140, 508, 783, 821, 838, 973
RseI	CAYNNNNRTG	2 cut(s)	704, 804
SacI	GAGCTC	1 cut(s)	32
SapI	GCTCTTC	1 cut(s)	795
SaqAI	TTAA	3 cut(s)	119, 240, 587
SatI	GCNGC	2 cut(s)	674, 694
Sau3AI	GATC	2 cut(s)	7, 456
Sau96I	GGNCC	2 cut(s)	575, 1035
ScaI	AGTACT	2 cut(s)	509, 784
SchI	GAGTC	1 cut(s)	909
ScrFI	CCNGG	3 cut(s)	318, 649, 723
SduI	GDGCHC	4 cut(s)	32, 81, 647, 855
SfaNI	GCATC	1 cut(s)	303
SfcI	CTRYAG	3 cut(s)	487, 511, 688
SinI	GGWCC	2 cut(s)	575, 1035
SmiMI	CAYNNNNRTG	2 cut(s)	704, 804
SmlI	CTYRAG	2 cut(s)	776, 1012
SmoI	CTYRAG	2 cut(s)	776, 1012
SphI	GCATGC	1 cut(s)	743
Sse9I	AATT	5 cut(s)	207, 243, 407, 451, 1003
SsiI	CCGC	3 cut(s)	216, 674, 770
SspMI	CTAG	6 cut(s)	275, 566, 665, 882, 897, 1061
SstI	GAGCTC	1 cut(s)	32
StyD4I	CCNGG	3 cut(s)	316, 647, 721
StyI	CCWWGG	4 cut(s)	253, 444, 498, 565
TaaI	ACNGT	4 cut(s)	196, 625, 642, 908
TaiI	ACGT	1 cut(s)	78
TaqI	TCGA	5 cut(s)	26, 150, 309, 459, 918
TasI	AATT	5 cut(s)	207, 243, 407, 451, 1003
TatI	WGTACW	3 cut(s)	507, 782, 837
TauI	GCSGC	1 cut(s)	676
TfiI	GAWTC	6 cut(s)	94, 234, 258, 403, 461, 885
Tru1I	TTAA	3 cut(s)	119, 240, 587
Tru9I	TTAA	3 cut(s)	119, 240, 587
TscAI	CASTG	2 cut(s)	369, 870
TseFI	GTSAC	1 cut(s)	806
TseI	GCWGC	1 cut(s)	693
Tsp45I	GTSAC	1 cut(s)	806
TspDTI	ATGAA	5 cut(s)	195, 328, 539, 944, 969
TspGWI	ACGGA	1 cut(s)	621
TspRI	CASTG	2 cut(s)	369, 870
VneI	GTGCAC	1 cut(s)	851
VpaK11BI	GGWCC	2 cut(s)	575, 1035
XapI	RAATTY	1 cut(s)	1003
XceI	RCATGY	1 cut(s)	743
XcmI	CCANNNNNNNNNTGG	1 cut(s)	655
XmaJI	CCTAGG	1 cut(s)	565
XspI	CTAG	6 cut(s)	275, 566, 665, 882, 897, 1061
ZrmI	AGTACT	2 cut(s)	509, 784
Zsp2I	ATGCAT	1 cut(s)	741

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.