AT4G31210

Topoisomerase C-terminal repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006256
Physical Location & Seq
Forward (+)
15165125 .. 15172807
7683 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G31210.3

Sequence Viewer

Length: 3855 bp
ATGCAGAGAACCATCTCACTTGCGGCTGCTAAGTCTTCTTCTTCGACTTCAGTTCTGTCTCTTCATCCTCTCATGGCTAAGTTGCAGTGCAGGGCGATTCAAAATTTCCCAGCTTCCTCTTCCTCTTCAGTAGTTCGGGTCGATAGAGTGTATAGAAATGTATCCCAGCTTCAGTTTAAAAGAGAAAATTCAAGTTGTCTTAAGTTAGCTTGTGCACTTCCTTCGCATCTAAGTCTTCTCGGTTCTCTTAGTTATGCAACACATTGGAGCAGCTCCACTTCAAGGGCCTTTGGATATAGTTTCAGACCGTTTGCGAGGAGATATTTTTCTCAAGTCGCTAGTACTGAGATTAAGGATAGCATTGTTGGTGGTGTTGAAAAGTTTGGTGGGAATAAAATAGGTTTCAAGAAATTCAATAAGAAGTGGAAGAAGCATAGGGTTTTGGCTTCCACAAAAGCTGAAGTGGTGGCTTCTACTGAACCAGTTATTGGAGATGTTAACAGTGGCATCAAGGCGAAACTATCAACTGCAGCTTCACCTGCTAGCAATGCTAAGCAAGCCTCTACTGTCAAAACAAAACGGCAGCCAAAGAGCAAGAAATTCGAAGATAAATCTTCTCCAACGGTATCGGTTTTGGAGACCGTCTCTGTAGACGAGAGTTTGCAAAGCTTTCCCAAGCCTAGACATTCGGGTTCCGGAAATCGGAAGTCTTCATCTGCTGAGTACAGTTCTCAGAAGGAGGTGGTAAAAAAAACCAATGTGGAGGGGCCCAAAAGTTCTACTCCATCGAATTCCATGTCAGAACAACAGCATTGGACCTCAACAAAAGCAAGCAATGCACCAAAACAGGAGCAGGATAACATTGTTGGTGGTGATGAGAAGGCTGGTGGGAATAAAGTAGGTTTCAAGAAATTTAACAAGAACCGAAAGAAGCATAATGTGTTGGCTTCCTCAGAAGCTGAAGTGGTGACTTCTACTGAACCAGTTATTGGAGATGGTAGCAGTGGCATCAAGGCGGAACTATCCACGGCAGCCTCACCTGCTAGCAATGGTAATCAAGCCACTACTGTCAAATCAAAACGGCGCCCCAAGAACAAGAAAGTCGAAGATAAATCTTCTTCAGTGGTACCAGTTTTGGAGGCGGTCTCTCTAGATGAGAGTCCGATAAGCGTTCCCAAGCCAAAACATTCTGGTTCTGGAAATCGGAAGTCTTCATCTGCTAAGAAGGAGGTGGCAAAAAATCACCCTGTGGAAGAGCCCAAAAGTCCTGCTCCATCAAATTCCAAGTCGGAACAACAGCATTTGAAGTCAACTAAAGCAAGTAAGGCACCAAAACAAAAGCTGGTTCCTCAACACATGAAAAACAGCATAGAACATCGAGGTCAAAATGCATCTAAACCGCTTTATCCGCCAAGTGGAAAATCTGTTATTGTCGTTGAGTCAATGACAAAAGCAAAGATTATTCAGGGTTATCTTGGTGACATGTATGAGGTTTTGCCAAGTTATGGTCATATCAGGGACCTGGCCACAAGGTCTGGTTCAGTGAGGCCAGATGACGATTTTAGCATGGTTTGGGAGGTTCCATCTTCCGCCTGGACTCATATTAAGAGCATTAAGGTTGCATTAAATGGAGCTGAAAATCTGATTCTTGCATCGGACCCAGATCGTGAAGGAGAGGCAATTGCCTGGCACATTATAGAGATGTTGCAGCAGCAGGGTGCCTTACATGAAAGTATGACAGTAGCAAGGGTTGTATTCCATGAGATAACTGAATCAGCAATAAAAAGTGCACTTCAATCTCCACGAGAAATCGACGGGGACTTGGTGCATGCTTATCTTGCACGGCGTGCTCTTGATTATCTCATCGGATTCAATATTTCACCACTACTATGGAGGAAACTTCCGGGTTGCCCGTCAGCTGGGCGGGTCCAGTCTGCTGCTTTGGCTCTTGTATGTGATAGAGAAAGTGAAATTGATGGATTTAAGCCTCAGGAGTACTGGACTGTAGGAATCAAAGTGAAAGGGAAAGATAATTCAGCCACCTTTTCTGCTCACTTGACCAGTTTAAATTCAAAAAGATTAAATCAGCTTTCTATCAGCTCTGAAGCAAATGCACAGGACATTGAGCAAAGGATCAAATCAGAAGGTTTTCTAGTGAAGGGCACTAAAACAAGTACTACACGGAAGAATCCGCCAACTCCGTACATAACATCAACCCTTCAGCAGGATGCTGCTAACAAATTACATTTTTCAACAGCACATACCATGAAGCTCGCTCAAAAATTATATGAGGGTGTCCAACTGTCCGATGGTAAATCAGCTGGTCTTATAACATACATGCGGACAGATGGTTTACATATAGCTGATGAAGCTATCAAAGATATACAGTCCTTGGTGGCAGAAAGGTATGGGAAGAATTTTACATCAGATAGTCCTCGTAAATATTTTAAAAAGGTTAAGAACGCTCAGGAGGCCCATGAAGCTATTAGACCTACTGATATACGTAGATTACCGTCAACGATTGCTAGCCTGCTTGATGCGGATTCTCTAAAATTATATACCTTGATATGGTCACGAGCTGTGGCATGTCAAATGGAGCCTGCTTCCATTGCGCAGATACAACTTGATATTGGCAATGCCTCTGAATCCATCATCTTCAGATCATCATGCTCAAAAGTTGAATTCCTTGGGTACCAGGCAGTTTACGAGGATCCTGAAGCTAAGGCAATCAAAAATAACGATAATGATCAGAGTAGTGAGCGTGAGGAAACTTTTAAAACTCTCAGTTCGTTGAAGGATGGAGATTTGCTACAGATTGGTGAAGTGGAGCTCAAGCAGCACCATACTCAGCACCCACCACGCTATTCTGAGGGCTCTCTGGTTAAAAAGCTCGAGGAGCTCGGGATAGGTAGACCCTCGACATATGCATCTATATTTAGGGTTTTACAGCACAGAAAGTACGTAACAATAAAGAATCGAGTCCTGTATCCGGAATTCCGTGCTAGGATGGTTTCAGCTTTTCTTACCCACTACTTTACTGAGATCACAGACTATAGTTTCACTGCGGATATGGAGACTGAGCTTGATAACGTTTCTGGTGGTGTAACTGAATGGAAAGGTCTCCTAAGAGACTACTGGACACGATTCAGCGCATATTGTAAACGTGTTGAAAATGTCCAACGCTCACAGGTGGAAAAAATGTTGGAAAAGAAATATGAGGACGTTCTGTTTTCTTTGCTTCCTTATCCCAGTAGGACATGCCCGAGTTGTATGGAAGGCACCTTAAGTTTCAAAGCAAGTAAGTTTGGTGCGGGGTATTTCATCGGTTGTGATCAGCACCCTTCATGCAAGTTCATTGCTAAAACGCTTTATGGAGAGGATGAAGATGAAGATGATCCTCCAAAGAATAACTGCGTAGAAGAGCCCAAATTGCTGGGTCTTCATCCCAATACCAGTGAGAAGGTTATTCTAAAGTGTGGACCCTATGGGCATTATGTACAGCTCGGTGAAGATAAAAAAGGGCACACACCAAAACGAGCAAATGCGGCCCATATAAAGGATGTGAACTCCATTACGCTTGAAAGTGCTCTCGAATTATTACGCTATCCTTTGACACTGGGAACCCACCCTGAAGATGGACAGCCTGTGGTCTTGAAGCTTAGTAAATCTGGATTTACTATTAAACATCGCCGCAATATGGCTACTGTTCCAAAGAACACGGAACCAGGTGAGGTTACTTTAGAGAGAGCAATGAAGCTCTTGTCTGGCAAAAATGTGAGAAAAAGTGGCAGACCTCCTAAGAGTATACTGCCTGAGGAAGAGAAAGGAGATGAAGAAGTTGTGGTAGCGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topoisom_bac Toprim Toprim_C_rpt zf-C4_Topoisom

Protein Analysis

1284

Amino Acids

141.56

Weight (kDa)

9.39

Isoelectric Point (pI)

47.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Toprim PF01751 472 - 585 7e-18 Toprim domain
Topoisom_bac PF01131 600 - 1037 6.4e-109 DNA topoisomerase
Zn_ribbon_Top1 PF01396 1081 - 1117 3.5e-07 Topoisomerase DNA binding C4 zinc finger
Toprim_C_rpt PF13368 1144 - 1199 1.1e-09 Topoisomerase C-terminal repeat
Toprim_C_rpt PF13368 1205 - 1254 2.4e-06 Topoisomerase C-terminal repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2330
AarI CACCTGC 2 cut(s) 547, 1048
Acc16I TGCGCA 1 cut(s) 2613
Acc36I ACCTGC 2 cut(s) 547, 1048
Acc65I GGTACC 2 cut(s) 1126, 2691
AccB1I GGYRCC 6 cut(s) 1083, 1126, 1327, 1718, 2691, 3278
AccB7I CCANNNNNTGG 3 cut(s) 488, 989, 1505
AccI GTMKAC 3 cut(s) 651, 2911, 3805
AccIII TCCGGA 2 cut(s) 695, 2989
AclI AACGTT 1 cut(s) 3090
AclWI GGATC 4 cut(s) 2141, 2705, 2718, 3389
AcoI YGGCCR 1 cut(s) 1524
AcyI GRCGYC 1 cut(s) 1084
AdeI CACNNNGTG 2 cut(s) 1249, 1847
AfaI GTAC 9 cut(s) 343, 725, 1128, 1997, 2176, 2204, 2693, 2960, 3498
AflII CTTAAG 2 cut(s) 200, 3283
AflIII ACRYGT 2 cut(s) 1482, 3163
AjnI CCWGG 5 cut(s) 1521, 1592, 1685, 2694, 3724
AjuI GAANNNNNNNTTGG 4 cut(s) 471, 503, 972, 1004
Alw21I GWGCWC 6 cut(s) 217, 1792, 1852, 2832, 2901, 3589
Alw26I GTCTC 7 cut(s) 63, 632, 649, 1150, 3068, 3123, 3125
Alw44I GTGCAC 2 cut(s) 213, 1788
AlwI GGATC 4 cut(s) 2141, 2705, 2718, 3389
AlwNI CAGNNNCTG 1 cut(s) 959
Ama87I CYCGRG 3 cut(s) 2891, 2900, 3262
Aor13HI TCCGGA 2 cut(s) 695, 2989
AoxI GGCC 6 cut(s) 285, 767, 1524, 1547, 2472, 3546
ApaI GGGCCC 1 cut(s) 771
ApaLI GTGCAC 2 cut(s) 213, 1788
ArsI GACNNNNNNTTYG 4 cut(s) 782, 814, 1271, 1303
Asp700I GAANNNNTTC 1 cut(s) 2148
Asp718I GGTACC 2 cut(s) 1126, 2691
AspLEI GCGC 3 cut(s) 1086, 2614, 3152
AsuC2I CCSGG 1 cut(s) 1905
AsuII TTCGAA 1 cut(s) 603
AsuNHI GCTAGC 3 cut(s) 542, 1043, 2525
AvaI CYCGRG 3 cut(s) 2891, 2900, 3262
AvaII GGWCC 5 cut(s) 816, 1519, 1657, 1927, 3479
AxyI CCTNAGG 2 cut(s) 1989, 3813
BaeGI GKGCMC 5 cut(s) 217, 771, 1792, 2165, 3525
BalI TGGCCA 1 cut(s) 1526
BamHI GGATCC 1 cut(s) 2710
BanI GGYRCC 6 cut(s) 1083, 1126, 1327, 1718, 2691, 3278
BanII GRGCYC 6 cut(s) 771, 1260, 2832, 2876, 2901, 3426
BarI GAAGNNNNNNTAC 2 cut(s) 153, 185
BauI CACGAG 2 cut(s) 1803, 2574
BbsI GAAGAC 5 cut(s) 27, 227, 702, 1203, 3431
Bbv12I GWGCWC 6 cut(s) 217, 1792, 1852, 2832, 2901, 3589
BceAI ACGGC 4 cut(s) 596, 1044, 1097, 1859
BcgI CGANNNNNNTGC 4 cut(s) 204, 238, 583, 617
BciT130I CCWGG 5 cut(s) 1523, 1594, 1687, 2696, 3726
BciVI GTATCC 2 cut(s) 172, 2997
BclI TGATCA 2 cut(s) 2746, 3331
BcnI CCSGG 1 cut(s) 1905
BcoDI GTCTC 7 cut(s) 63, 632, 649, 1150, 3068, 3123, 3125
BfaI CTAG 8 cut(s) 339, 543, 681, 1044, 1151, 2153, 2526, 3003
BfmI CTRYAG 5 cut(s) 528, 648, 2004, 2810, 3052
BfoI RGCGCY 1 cut(s) 1087
BfrI CTTAAG 2 cut(s) 200, 3283
BfuAI ACCTGC 2 cut(s) 547, 1048
BfuI GTATCC 2 cut(s) 172, 2997
BlpI GCTNAGC 1 cut(s) 552
BmcAI AGTACT 3 cut(s) 343, 1997, 2176
Bme1390I CCNGG 6 cut(s) 1523, 1594, 1687, 1905, 2696, 3726
Bme18I GGWCC 5 cut(s) 816, 1519, 1657, 1927, 3479
BmeT110I CYCGRG 3 cut(s) 2891, 2900, 3262
BmrFI CCNGG 6 cut(s) 1523, 1594, 1687, 1905, 2696, 3726
BmrI ACTGGG 2 cut(s) 3243, 3626
BmsI GCATC 8 cut(s) 235, 516, 1017, 1400, 1661, 2218, 2527, 2936
BmtI GCTAGC 3 cut(s) 546, 1047, 2529
BmuI ACTGGG 2 cut(s) 3243, 3626
BpiI GAAGAC 5 cut(s) 27, 227, 702, 1203, 3431
BplI GAGNNNNNCTC 4 cut(s) 629, 661, 1130, 1162
Bpu10I CCTNAGC 2 cut(s) 2466, 2721
Bpu1102I GCTNAGC 1 cut(s) 552
Bpu14I TTCGAA 1 cut(s) 603
BpuEI CTTGAG 2 cut(s) 315, 2816
BpuMI CCSGG 1 cut(s) 1905
BsaAI YACGTR 2 cut(s) 2504, 2962
BsaBI GATNNNNATC 1 cut(s) 2745
BsaHI GRCGYC 1 cut(s) 1084
BsaI GGTCTC 3 cut(s) 632, 1150, 3125
BsaJI CCNNGG 3 cut(s) 1026, 2391, 2686
BsaWI WCCGGW 2 cut(s) 695, 2989
Bse21I CCTNAGG 2 cut(s) 1989, 3813
Bse3DI GCAATG 7 cut(s) 553, 841, 1054, 2607, 2641, 3354, 3756
Bse8I GATNNNNATC 1 cut(s) 2745
BseAI TCCGGA 2 cut(s) 695, 2989
BseBI CCWGG 5 cut(s) 1523, 1594, 1687, 2696, 3726
BseDI CCNNGG 3 cut(s) 1026, 2391, 2686
BseGI GGATG 7 cut(s) 64, 2233, 2803, 3012, 3385, 3442, 3565
BseJI GATNNNNATC 1 cut(s) 2745
BseMI GCAATG 7 cut(s) 553, 841, 1054, 2607, 2641, 3354, 3756
BseRI GAGGAG 2 cut(s) 331, 2909
BseSI GKGCMC 5 cut(s) 217, 771, 1792, 2165, 3525
BseYI CCCAGC 4 cut(s) 109, 165, 1919, 3433
BsgI GTGCAG 1 cut(s) 109
BshFI GGCC 6 cut(s) 287, 769, 1526, 1549, 2474, 3548
BshNI GGYRCC 6 cut(s) 1083, 1126, 1327, 1718, 2691, 3278
BsiHKAI GWGCWC 6 cut(s) 217, 1792, 1852, 2832, 2901, 3589
BsiHKCI CYCGRG 3 cut(s) 2891, 2900, 3262
BsiSI CCGG 3 cut(s) 696, 1904, 2990
BslFI GGGAC 2 cut(s) 1532, 1832
BsmAI GTCTC 7 cut(s) 63, 632, 649, 1150, 3068, 3123, 3125
BsmBI CGTCTC 1 cut(s) 649
BsmFI GGGAC 2 cut(s) 1532, 1832
BsnI GGCC 6 cut(s) 287, 769, 1526, 1549, 2474, 3548
Bso31I GGTCTC 3 cut(s) 632, 1150, 3125
BsoBI CYCGRG 3 cut(s) 2891, 2900, 3262
Bsp119I TTCGAA 1 cut(s) 603
Bsp120I GGGCCC 1 cut(s) 767
Bsp13I TCCGGA 2 cut(s) 695, 2989
Bsp1407I TGTACA 1 cut(s) 3496
Bsp143I GATC 8 cut(s) 1663, 2133, 2660, 2710, 2746, 3042, 3331, 3394
Bsp1720I GCTNAGC 1 cut(s) 552
BspANI GGCC 6 cut(s) 287, 769, 1526, 1549, 2474, 3548
BspEI TCCGGA 2 cut(s) 695, 2989
BspMAI CTGCAG 1 cut(s) 532
BspMI ACCTGC 2 cut(s) 547, 1048
BspOI GCTAGC 3 cut(s) 546, 1047, 2529
BspPI GGATC 4 cut(s) 2141, 2705, 2718, 3389
BspQI GCTCTTC 2 cut(s) 1248, 3414
BspT104I TTCGAA 1 cut(s) 603
BspT107I GGYRCC 6 cut(s) 1083, 1126, 1327, 1718, 2691, 3278
BspTI CTTAAG 2 cut(s) 200, 3283
BspTNI GGTCTC 3 cut(s) 632, 1150, 3125
BsrDI GCAATG 7 cut(s) 553, 841, 1054, 2607, 2641, 3354, 3756
BsrGI TGTACA 1 cut(s) 3496
BssECI CCNNGG 3 cut(s) 1026, 2391, 2686
BssMI GATC 8 cut(s) 1663, 2133, 2660, 2710, 2746, 3042, 3331, 3394
BssNAI GTATAC 1 cut(s) 3806
BssNI GRCGYC 1 cut(s) 1084
BssSI CACGAG 2 cut(s) 1803, 2574
BssT1I CCWWGG 2 cut(s) 2391, 2686
Bst1107I GTATAC 1 cut(s) 3806
Bst2BI CACGAG 2 cut(s) 1803, 2574
Bst2UI CCWGG 5 cut(s) 1523, 1594, 1687, 2696, 3726
Bst6I CTCTTC 6 cut(s) 66, 124, 130, 1248, 3414, 3813
BstACI GRCGYC 1 cut(s) 1084
BstAFI CTTAAG 2 cut(s) 200, 3283
BstAPI GCANNNNNTGC 2 cut(s) 836, 1847
BstAUI TGTACA 1 cut(s) 3496
BstBAI YACGTR 2 cut(s) 2504, 2962
BstBI TTCGAA 1 cut(s) 603
BstDSI CCRYGG 1 cut(s) 1026
BstENI CCTNNNNNAGG 1 cut(s) 2222
BstF5I GGATG 7 cut(s) 64, 2233, 2803, 3012, 3385, 3442, 3565
BstH2I RGCGCY 1 cut(s) 1087
BstHHI GCGC 3 cut(s) 1086, 2614, 3152
BstKTI GATC 8 cut(s) 1666, 2136, 2663, 2713, 2749, 3045, 3334, 3397
BstMAI GTCTC 7 cut(s) 63, 632, 649, 1150, 3068, 3123, 3125
BstMBI GATC 8 cut(s) 1663, 2133, 2660, 2710, 2746, 3042, 3331, 3394
BstNI CCWGG 5 cut(s) 1523, 1594, 1687, 2696, 3726
BstNSI RCATGY 5 cut(s) 1486, 1832, 2341, 2589, 3261
BstSCI CCNGG 6 cut(s) 1521, 1592, 1685, 1903, 2694, 3724
BstSFI CTRYAG 5 cut(s) 528, 648, 2004, 2810, 3052
BstSLI GKGCMC 5 cut(s) 217, 771, 1792, 2165, 3525
BstSNI TACGTA 2 cut(s) 2504, 2962
BstV2I GAAGAC 5 cut(s) 27, 227, 702, 1203, 3431
BstX2I RGATCY 1 cut(s) 2710
BstXI CCANNNNNNTGG 2 cut(s) 1890, 3433
BstYI RGATCY 1 cut(s) 2710
BstZ17I GTATAC 1 cut(s) 3806
Bsu36I CCTNAGG 2 cut(s) 1989, 3813
BsuI GTATCC 2 cut(s) 172, 2997
BsuRI GGCC 6 cut(s) 287, 769, 1526, 1549, 2474, 3548
BtgI CCRYGG 1 cut(s) 1026
BtgZI GCGATG 1 cut(s) 3671
BtsCI GGATG 7 cut(s) 64, 2233, 2803, 3012, 3385, 3442, 3565
BtsI GCAGTG 3 cut(s) 92, 1009, 3060
BtsIMutI CAGTG 8 cut(s) 92, 508, 1009, 1128, 1548, 3060, 3460, 3614
BveI ACCTGC 2 cut(s) 547, 1048
CaiI CAGNNNCTG 1 cut(s) 959
CfoI GCGC 3 cut(s) 1086, 2614, 3152
CsiI ACCWGGT 1 cut(s) 3724
Csp6I GTAC 9 cut(s) 342, 724, 1127, 1996, 2175, 2203, 2692, 2959, 3497
CviQI GTAC 9 cut(s) 342, 724, 1127, 1996, 2175, 2203, 2692, 2959, 3497
DinI GGCGCC 1 cut(s) 1085
DpnI GATC 8 cut(s) 1665, 2135, 2662, 2712, 2748, 3044, 3333, 3396
DpnII GATC 8 cut(s) 1663, 2133, 2660, 2710, 2746, 3042, 3331, 3394
DraI TTTAAA 4 cut(s) 178, 2067, 2449, 2776
DraIII CACNNNGTG 2 cut(s) 1249, 1847
EaeI YGGCCR 1 cut(s) 1524
Eam1104I CTCTTC 6 cut(s) 66, 124, 130, 1248, 3414, 3813
EarI CTCTTC 6 cut(s) 66, 124, 130, 1248, 3414, 3813
EciI GGCGGA 4 cut(s) 1031, 1398, 1579, 2181
Ecl136II GAGCTC 2 cut(s) 2830, 2899
Eco105I TACGTA 2 cut(s) 2504, 2962
Eco130I CCWWGG 2 cut(s) 2391, 2686
Eco24I GRGCYC 6 cut(s) 771, 1260, 2832, 2876, 2901, 3426
Eco31I GGTCTC 3 cut(s) 632, 1150, 3125
Eco47I GGWCC 5 cut(s) 816, 1519, 1657, 1927, 3479
Eco53kI GAGCTC 2 cut(s) 2830, 2899
Eco81I CCTNAGG 2 cut(s) 1989, 3813
Eco88I CYCGRG 3 cut(s) 2891, 2900, 3262
EcoICRI GAGCTC 2 cut(s) 2830, 2899
EcoNI CCTNNNNNAGG 1 cut(s) 2222
EcoO109I RGGNCCY 3 cut(s) 285, 767, 1519
EcoRI GAATTC 3 cut(s) 790, 2681, 2993
EcoRII CCWGG 5 cut(s) 1521, 1592, 1685, 2694, 3724
EcoT14I CCWWGG 2 cut(s) 2391, 2686
EcoT22I ATGCAT 2 cut(s) 1393, 2929
EcoT38I GRGCYC 6 cut(s) 771, 1260, 2832, 2876, 2901, 3426
EgeI GGCGCC 1 cut(s) 1085
EheI GGCGCC 1 cut(s) 1085
ErhI CCWWGG 2 cut(s) 2391, 2686
Esp3I CGTCTC 1 cut(s) 649
FaqI GGGAC 2 cut(s) 1532, 1832
FauI CCCGC 2 cut(s) 1917, 3304
FauNDI CATATG 1 cut(s) 2923
FbaI TGATCA 2 cut(s) 2746, 3331
FblI GTMKAC 3 cut(s) 651, 2911, 3805
FokI GGATG 7 cut(s) 51, 2240, 2810, 3019, 3392, 3429, 3572
FriOI GRGCYC 6 cut(s) 771, 1260, 2832, 2876, 2901, 3426
FspBI CTAG 8 cut(s) 339, 543, 681, 1044, 1151, 2153, 2526, 3003
FspI TGCGCA 1 cut(s) 2613
GlaI GCGC 3 cut(s) 1085, 2613, 3151
GsaI CCCAGC 4 cut(s) 113, 169, 1923, 3437
HaeII RGCGCY 1 cut(s) 1087
HaeIII GGCC 6 cut(s) 287, 769, 1526, 1549, 2474, 3548
HapII CCGG 3 cut(s) 696, 1904, 2990
HhaI GCGC 3 cut(s) 1086, 2614, 3152
Hin1I GRCGYC 1 cut(s) 1084
Hin6I GCGC 3 cut(s) 1084, 2612, 3150
HinP1I GCGC 3 cut(s) 1084, 2612, 3150
HincII GTYRAC 3 cut(s) 499, 1311, 2517
HindII GTYRAC 3 cut(s) 499, 1311, 2517
HindIII AAGCTT 2 cut(s) 667, 3656
HpaI GTTAAC 1 cut(s) 499
HpaII CCGG 3 cut(s) 696, 1904, 2990
Hpy99I CGWCG 1 cut(s) 1817
HpyCH4IV ACGT 5 cut(s) 2503, 2961, 3090, 3163, 3222
HpySE526I ACGT 5 cut(s) 2503, 2961, 3090, 3163, 3222
Hsp92I GRCGYC 1 cut(s) 1084
HspAI GCGC 3 cut(s) 1084, 2612, 3150
KasI GGCGCC 1 cut(s) 1083
Kpn2I TCCGGA 2 cut(s) 695, 2989
KpnI GGTACC 2 cut(s) 1130, 2695
Ksp22I TGATCA 2 cut(s) 2746, 3331
KspAI GTTAAC 1 cut(s) 499
Kzo9I GATC 8 cut(s) 1663, 2133, 2660, 2710, 2746, 3042, 3331, 3394
LguI GCTCTTC 2 cut(s) 1248, 3414
LmnI GCTCC 8 cut(s) 267, 278, 850, 1276, 1631, 2596, 2827, 2896
LweI GCATC 8 cut(s) 235, 516, 1017, 1400, 1661, 2218, 2527, 2936
MabI ACCWGGT 1 cut(s) 3724
MaeI CTAG 8 cut(s) 339, 543, 681, 1044, 1151, 2153, 2526, 3003
MaeII ACGT 5 cut(s) 2503, 2961, 3090, 3163, 3222
MaeIII GTNAC 6 cut(s) 967, 1478, 2571, 2962, 3103, 3733
MalI GATC 8 cut(s) 1665, 2135, 2662, 2712, 2748, 3044, 3333, 3396
MboI GATC 8 cut(s) 1663, 2133, 2660, 2710, 2746, 3042, 3331, 3394
MfeI CAATTG 1 cut(s) 1680
MflI RGATCY 1 cut(s) 2710
MlsI TGGCCA 1 cut(s) 1526
MluNI TGGCCA 1 cut(s) 1526
Mly113I GGCGCC 1 cut(s) 1084
MlyI GAGTC 4 cut(s) 1168, 1448, 1591, 2988
MmeI TCCRAC 5 cut(s) 644, 1269, 2323, 3183, 3202
Mox20I TGGCCA 1 cut(s) 1526
Mph1103I ATGCAT 2 cut(s) 1393, 2929
MroI TCCGGA 2 cut(s) 695, 2989
MroXI GAANNNNTTC 1 cut(s) 2148
MscI TGGCCA 1 cut(s) 1526
MslI CAYNNNNRTG 1 cut(s) 1888
Msp20I TGGCCA 1 cut(s) 1526
MspA1I CMGCKG 2 cut(s) 1919, 2321
MspCI CTTAAG 2 cut(s) 200, 3283
MspI CCGG 3 cut(s) 696, 1904, 2990
MspR9I CCNGG 6 cut(s) 1523, 1594, 1687, 1905, 2696, 3726
MunI CAATTG 1 cut(s) 1680
MvaI CCWGG 5 cut(s) 1523, 1594, 1687, 2696, 3726
NarI GGCGCC 1 cut(s) 1084
NciI CCSGG 1 cut(s) 1905
NdeI CATATG 1 cut(s) 2923
NdeII GATC 8 cut(s) 1663, 2133, 2660, 2710, 2746, 3042, 3331, 3394
NheI GCTAGC 3 cut(s) 542, 1043, 2525
NmuCI GTSAC 3 cut(s) 967, 1478, 2571
NsbI TGCGCA 1 cut(s) 2613
NsiI ATGCAT 2 cut(s) 1393, 2929
NspI RCATGY 5 cut(s) 1486, 1832, 2341, 2589, 3261
NspV TTCGAA 1 cut(s) 603
PaeI GCATGC 1 cut(s) 1832
PaeR7I CTCGAG 1 cut(s) 2891
PaqCI CACCTGC 2 cut(s) 547, 1048
PciI ACATGT 1 cut(s) 1482
PciSI GCTCTTC 2 cut(s) 1248, 3414
PdmI GAANNNNTTC 1 cut(s) 2148
PflMI CCANNNNNTGG 3 cut(s) 488, 989, 1505
PleI GAGTC 4 cut(s) 1167, 1447, 1591, 2987
PluTI GGCGCC 1 cut(s) 1087
PpsI GAGTC 4 cut(s) 1167, 1447, 1591, 2987
Ppu21I YACGTR 2 cut(s) 2504, 2962
PpuMI RGGWCCY 1 cut(s) 1519
PscI ACATGT 1 cut(s) 1482
PsiI TTATAA 1 cut(s) 2330
Psp124BI GAGCTC 2 cut(s) 2832, 2901
Psp1406I AACGTT 1 cut(s) 3090
Psp5II RGGWCCY 1 cut(s) 1519
Psp6I CCWGG 5 cut(s) 1521, 1592, 1685, 2694, 3724
PspFI CCCAGC 4 cut(s) 109, 165, 1919, 3433
PspGI CCWGG 5 cut(s) 1521, 1592, 1685, 2694, 3724
PspOMI GGGCCC 1 cut(s) 767
PspPPI RGGWCCY 1 cut(s) 1519
PspXI VCTCGAGB 1 cut(s) 2891
PstI CTGCAG 1 cut(s) 532
PstNI CAGNNNCTG 1 cut(s) 959
PsuI RGATCY 1 cut(s) 2710
PvuII CAGCTG 2 cut(s) 1919, 2321
RsaI GTAC 9 cut(s) 343, 725, 1128, 1997, 2176, 2204, 2693, 2960, 3498
RsaNI GTAC 9 cut(s) 342, 724, 1127, 1996, 2175, 2203, 2692, 2959, 3497
RseI CAYNNNNRTG 1 cut(s) 1888
SacI GAGCTC 2 cut(s) 2832, 2901
SapI GCTCTTC 2 cut(s) 1248, 3414
Sau3AI GATC 8 cut(s) 1663, 2133, 2660, 2710, 2746, 3042, 3331, 3394
ScaI AGTACT 3 cut(s) 343, 1997, 2176
SchI GAGTC 4 cut(s) 1168, 1448, 1591, 2988
ScrFI CCNGG 6 cut(s) 1523, 1594, 1687, 1905, 2696, 3726
SexAI ACCWGGT 1 cut(s) 3724
SfaNI GCATC 8 cut(s) 235, 516, 1017, 1400, 1661, 2218, 2527, 2936
SfcI CTRYAG 5 cut(s) 528, 648, 2004, 2810, 3052
SfoI GGCGCC 1 cut(s) 1085
Sfr274I CTCGAG 1 cut(s) 2891
SfuI TTCGAA 1 cut(s) 603
SinI GGWCC 5 cut(s) 816, 1519, 1657, 1927, 3479
SlaI CTCGAG 1 cut(s) 2891
SmiMI CAYNNNNRTG 1 cut(s) 1888
SmlI CTYRAG 5 cut(s) 200, 330, 2831, 2891, 3283
SmoI CTYRAG 5 cut(s) 200, 330, 2831, 2891, 3283
SnaBI TACGTA 2 cut(s) 2504, 2962
SphI GCATGC 1 cut(s) 1832
SspDI GGCGCC 1 cut(s) 1083
SspI AATATT 2 cut(s) 1876, 2444
SspMI CTAG 8 cut(s) 339, 543, 681, 1044, 1151, 2153, 2526, 3003
SstI GAGCTC 2 cut(s) 2832, 2901
StyD4I CCNGG 6 cut(s) 1521, 1592, 1685, 1903, 2694, 3724
StyI CCWWGG 2 cut(s) 2391, 2686
TaiI ACGT 5 cut(s) 2506, 2964, 3093, 3166, 3225
TatI WGTACW 5 cut(s) 341, 723, 1995, 2174, 3496
TauI GCSGC 3 cut(s) 26, 3548, 3693
TscAI CASTG 8 cut(s) 92, 508, 1009, 1128, 1548, 3067, 3460, 3621
TseFI GTSAC 3 cut(s) 967, 1478, 2571
Tsp45I GTSAC 3 cut(s) 967, 1478, 2571
TspGWI ACGGA 4 cut(s) 2190, 2197, 2987, 3734
TspRI CASTG 8 cut(s) 92, 508, 1009, 1128, 1548, 3067, 3460, 3621
Van91I CCANNNNNTGG 3 cut(s) 488, 989, 1505
Vha464I CTTAAG 2 cut(s) 200, 3283
VneI GTGCAC 2 cut(s) 213, 1788
VpaK11BI GGWCC 5 cut(s) 816, 1519, 1657, 1927, 3479
XagI CCTNNNNNAGG 1 cut(s) 2222
XbaI TCTAGA 1 cut(s) 1150
XceI RCATGY 5 cut(s) 1486, 1832, 2341, 2589, 3261
XcmI CCANNNNNNNNNTGG 3 cut(s) 1590, 2306, 3632
XhoI CTCGAG 1 cut(s) 2891
XmiI GTMKAC 3 cut(s) 651, 2911, 3805
XmnI GAANNNNTTC 1 cut(s) 2148
XspI CTAG 8 cut(s) 339, 543, 681, 1044, 1151, 2153, 2526, 3003
ZrmI AGTACT 3 cut(s) 343, 1997, 2176
Zsp2I ATGCAT 2 cut(s) 1393, 2929
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.