Rroxscaffold_7G00163020

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006256
Physical Location & Seq
Reverse (-)
5358322 .. 5370558
12237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00163020.1

Sequence Viewer

Length: 3489 bp
ATGATTCATTTCCATAGGACCTTCTCATTGACAGCTCCTTCGTCCTCTTCATTCATGTTCTCATACCCTTCAACTTCTTCAGCTCTCAGACTCCGCCTCTCCATGGCCAAGCTGCAGTGTATGGCGTTTGAGCATTATCCGGGGACTTGCTTGTCGTTTTCTCCAGTTTTGACATTGAATAATTACCGAAACTATTCCCAGCTTAGGTTTAGAAAATCCAGAGTTTCTTGCATGATCACGTCCTACAGTGCGAAAGTTGGGCTGACTTTTGCTCCCCTGAGAAGAAATGGTGATGGATTTCAACGTGCTTTTAGCATTGATTTACACTTAAGACCGCGTGCCCTTAATGCTTTGGGGACTGCTAATGTTATGAGTCGTGGGTTTTTTGGAAATGGCTGTAGATCGAGTTTATTGGATTCTAGAGTTGGAGATTTTTCCAAGAGGTTTTTGTCTCAAGTTCCGGATGTGACAAGTAAAGTAGAGAATGTGGGCTCCCAAGATGGCAGGAGGCCTGGAAATAGAAAATGGTTCAAAATAATGATTAGCAATCGCAAGAAATTGAAAAAGGCATTGGCTACACAAACTGAAACTGAAGCTACCGTTTCGAGCATCTCCAAAGATGATAATTTAAAAGTTTCAAATGAAAAGCAGGTTGGAAGTGAGAAGGAGGATGAGAAGCTTGGATCATCAACTTCAGATTCGCCTATAAGCAAAACTAAAGAGGTCTCTAAACCAAAAGCAAAAAAGAGGTCGAAAAGCAAGAAAACTAAAGAACAAGATTCTGCTGCTAATGCTATAGCAGAAGCTGCTTCGACACAAAGTTCTGAAAGTACTTCTCAAACAAAGAAATCTGGCAGGAACAAAAAAGGAGCAAAGTCTGCACAGGATTCAGAGACTCCAGTAGACCTTGCTGATGGTTCAACAACAAAACCCCAGAGAAACAAGAAAGGTGAGAAGGAAAAATCTATAAATTCAGCTGCTAAGTCACCACAGAAAATTATTCCACAAGAAATGGGAAAATTGAAATCTCCTGGAAAAATGACAACGAAGCAGCTGTACCCCGCAACTGGGAAAACTGTTGTGGTGGTGGAGTCTGTTACAAAGGCGAAGGTTATTCAGGGTTATCTTGGTGATATGTTTGAAGTGCTGCCTAGCTATGGTCATGTGAGGGACTTGGCAGCTAGGTCAGGATCTGTGCGGCCCGATGATGATTTTAGTATGGTGTGGGAAGTTCCATCTGCTGCCTGGACTCATCTTAAGAGCATAAAGGTCGCCTTAAGTGGTGCAGAAAATCTTATCCTTGCATCAGATCCTGATCGTGAAGGAGAGGCTATCGCTTGGCATATTATTGAGATGCTGCAACAACAGGATGCTTTACGTAAAGATATTTATGTAGCAAGGGTTGTCTTTCATGAAATCACTGAGGCCTCCATCAAAAGTGCTCTGCAGGCTCCAAGAGAAATTGATCAAAATTTGGTCCATTCTTACCTTGCACGGCGTGCTCTTGATTATTTGATCGGATTTAATATTTCACCGTTGCTATGGAGGAAACTGCCAAGTTGCCAGTCTGCCGGGCGAGTCCAATCTGCTGCACTAGCTCTTGTATGCGACAGGGAAATGGAAATCGACAGATTTAAACCACAAGAATATTGGACTATTGATATTGAATTGAACAAAAAAGAACCAGATTCGTCAGTGAATGCCATATCCTTGCCAGCGCACTTGACCCATTTTGATTCCAAAAAGTTGGACCAATTTTCTATCAGTTCCCATACAGAGGCAAAAGATATTGCGCAGCAAATTAATGCAGAAGCTTTTAAAGTTATCAGCTCTAAGAGAAGCAAAATGCGGAAGAACCCTCCTACGCCTTATATAACATCAACACTTCAGCAGGATGCCGCAAACAAATTACATTTCAGTGCAACATATACAATGAAGCTTGCACAGAAGCTTTATGAGGGGGTTCAATTATCTGATGGCAAGTCAATGGGTTTGATCACATACACCAGAACAGATGGACTACACATTTCAGATGAAGCAGTAGAAGGTGTTCGCTCCTTGGTCATCAAAAGGTATGGACAGAACTTTGCATCAGCTACTCCACGCAAGTTTTTTAAGAAGGTGAAGAATGCTCAGGAGGCCCATGAAGCAATCAGACCTACTGATGTTCAAAGATTTCCATTGTTGCTCAGAGGGATACTTGATGAAGATTCATTAAAGTTGTACACTCTTATTTGGTGTCGGACAGTTGCATGCCAAATGGAGCCTGCTACAATTGAGCAGATACAACTTGATATTGGAAATGCTGGTGAGTCCATTGTTTTCCGATCTGCATGTTCAAGAGTTCAGTTCCTTGGATATCAGGCAGTTTTTCAGGACGTGGAAGCTGGAGCCGTCAAATATAAAGAAAATGAAGGGATCAACCGGGATGAAGCTTTTGCACTTCTTGATTCACTGAAGGTTGGCAGTCCAGTGCATCTTGGTGAAGTGGAACTCAAGCAACACCATACCCAACCACCTCCACGCTACTCTGAGGCATCATTGGTTAAGAAATTAGAGGAGCTTGGGATTGGAAGACCTTCAACATATGCAAGCACTATAAAGGACAGAAACTATCTGACAGTGAAAAGCCGTGTTTTACATCCAGAGTTTCGTGGTCGTATGCTTGATAATGTCTCTGCTGGCTTAACTGAATGGAAAGGCCTCCTAAGAGATTACTGGACTCGATTCAGTGGTTATTGTGAGCGTACCAGTAATGTGACTATCCATCAGGTGGAGAAAATGATGGAAAAAAATTTGGAGACTTCTTGTTTGCTACTCTCCCTGACCAAAGCAGGACATGTCCAAGAGGAAAGTGTTTTCTTGAGCACAGTTTCTAAGGACTTTTATAATCTTGATATGTGTTCCAGCTGTAATGAAGGAACTTTGATCTTCAAAGTGAGTCGATTCGGTGCAGGCTATTTCATAGGTTGTGATACACACCCAAAGTGCAAGTACATTGCTAAGACATTGTATGGTAATGAAGAAGAAGACGACAATACTCCGAAGAACAACAGGGTGGAGGAACCAAAATTGTTAGGTTTATCTCCTGGTTCCAATGAAAAGGTTCTTTTGAAGACTGGTCCTTATGGATCGTATGTACAACTTGGTGAAGACAGGAAGGGATACTCACCTAAAAGAGCCTCTATAGCACATATCAAGGATGTGAACTCAGTAACCGTTGAAGTTGCTCTGGAGTTGATGCGATATCCCGTGACATTGGGGAACCATCCGAAGGATGGGCTACCAGTGATTTTAAAGATTGCAAGGTCCGGATATATAGTTAAACATCGACGCACAATGGCTTCTCTAACGAAGAATCAGGAACCTAATAAATTAACCCTGGAGGACGCATTGGAACTTTTGTCTGGGAAAAATACACGGAAAATTGGTCGACCTAAGGGAAAATCAAAGGTTGAAGAAAAAGGAGAAGCTATAGAAGCCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topoisom_bac Toprim Toprim_C_rpt zf-C4_Topoisom

Protein Analysis

1162

Amino Acids

129.5

Weight (kDa)

9.44

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Toprim PF01751 359 - 472 1.9e-16 Toprim domain
Topoisom_bac PF01131 488 - 891 8.1e-98 DNA topoisomerase
Zn_ribbon_Top1 PF01396 968 - 1002 1.4e-06 Topoisomerase DNA binding C4 zinc finger
Toprim_C_rpt PF13368 1030 - 1084 1e-08 Topoisomerase C-terminal repeat
Toprim_C_rpt PF13368 1090 - 1141 1.1e-07 Topoisomerase C-terminal repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2889
AasI GACNNNNNNGTC 1 cut(s) 151
Acc16I TGCGCA 1 cut(s) 1794
Acc36I ACCTGC 1 cut(s) 640
AccB7I CCANNNNNTGG 1 cut(s) 2773
AccI GTMKAC 2 cut(s) 903, 3433
AccII CGCG 1 cut(s) 337
AccIII TCCGGA 2 cut(s) 460, 3311
AciI CCGC 6 cut(s) 94, 335, 1062, 1198, 1849, 1899
AclWI GGATC 5 cut(s) 691, 1198, 1304, 2426, 3139
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 3 cut(s) 970, 1471, 2794
AcuI CTGAAG 5 cut(s) 63, 612, 678, 1871, 2477
AdeI CACNNNGTG 1 cut(s) 1499
AfaI GTAC 6 cut(s) 832, 1058, 2225, 2749, 2996, 3141
AfiI CCNNNNNNNGG 9 cut(s) 103, 204, 1067, 1068, 1157, 1777, 2773, 3274, 3278
AflII CTTAAG 3 cut(s) 328, 1256, 1276
AflIII ACRYGT 1 cut(s) 2839
AjiI CACGTC 2 cut(s) 240, 2380
AjnI CCWGG 5 cut(s) 511, 1030, 1244, 3088, 3381
AjuI GAANNNNNNNTTGG 4 cut(s) 554, 586, 636, 668
Alw21I GWGCWC 3 cut(s) 1444, 1504, 2870
Alw26I GTCTC 5 cut(s) 456, 730, 887, 2680, 2795
AlwI GGATC 5 cut(s) 691, 1198, 1304, 2426, 3139
AlwNI CAGNNNCTG 3 cut(s) 806, 1193, 1313
Aor13HI TCCGGA 2 cut(s) 460, 3311
AoxI GGCC 6 cut(s) 105, 509, 1199, 1425, 2139, 2701
ApoI RAATTY 3 cut(s) 970, 1471, 2794
ArsI GACNNNNNNTTYG 6 cut(s) 22, 54, 734, 766, 2794, 2826
AseI ATTAAT 1 cut(s) 1803
Asp700I GAANNNNTTC 4 cut(s) 193, 2049, 2578, 3105
AspLEI GCGC 2 cut(s) 1720, 1795
AspS9I GGNCC 7 cut(s) 18, 1200, 1477, 1750, 2140, 3122, 3309
AsuC2I CCSGG 3 cut(s) 141, 1573, 2426
AvaII GGWCC 5 cut(s) 18, 1477, 1750, 3122, 3309
AxyI CCTNAGG 1 cut(s) 3438
BaeGI GKGCMC 1 cut(s) 343
BalI TGGCCA 1 cut(s) 107
BanII GRGCYC 1 cut(s) 494
BarI GAAGNNNNNNTAC 2 cut(s) 1040, 1072
BbsI GAAGAC 4 cut(s) 2581, 3036, 3122, 3159
Bbv12I GWGCWC 3 cut(s) 1444, 1504, 2870
BceAI ACGGC 3 cut(s) 1511, 2378, 2616
BcgI CGANNNNNNTGC 2 cut(s) 1252, 1286
BciT130I CCWGG 5 cut(s) 513, 1032, 1246, 3090, 3383
BciVI GTATCC 2 cut(s) 2190, 3158
BclI TGATCA 3 cut(s) 234, 1465, 1995
BcnI CCSGG 3 cut(s) 141, 1573, 2426
BcoDI GTCTC 5 cut(s) 456, 730, 887, 2680, 2795
BfaI CTAG 4 cut(s) 420, 1152, 1182, 1595
BfmI CTRYAG 7 cut(s) 113, 244, 397, 795, 1445, 3186, 3474
BfrI CTTAAG 3 cut(s) 328, 1256, 1276
BfuAI ACCTGC 1 cut(s) 640
BfuI GTATCC 2 cut(s) 2190, 3158
BmcAI AGTACT 1 cut(s) 832
Bme1390I CCNGG 8 cut(s) 141, 513, 1032, 1246, 1573, 2426, 3090, 3383
Bme18I GGWCC 5 cut(s) 18, 1477, 1750, 3122, 3309
BmgBI CACGTC 2 cut(s) 240, 2380
BmgT120I GGNCC 7 cut(s) 18, 1200, 1477, 1750, 2140, 3122, 3309
BmiI GGNNCC 8 cut(s) 493, 1452, 2265, 2392, 3066, 3094, 3266, 3366
BmrFI CCNGG 8 cut(s) 141, 513, 1032, 1246, 1573, 2426, 3090, 3383
BmrI ACTGGG 1 cut(s) 1077
BmsI GCATC 9 cut(s) 618, 1313, 1344, 1360, 1885, 2099, 2485, 2546, 3231
BmuI ACTGGG 1 cut(s) 1077
BpiI GAAGAC 4 cut(s) 2581, 3036, 3122, 3159
BpmI CTGGAG 5 cut(s) 147, 882, 2409, 3254, 3404
Bpu10I CCTNAGC 2 cut(s) 203, 2133
BpuEI CTTGAG 3 cut(s) 438, 2480, 2884
BpuMI CCSGG 3 cut(s) 141, 1573, 2426
BsaAI YACGTR 1 cut(s) 1379
BsaBI GATNNNNATC 1 cut(s) 1314
BsaI GGTCTC 1 cut(s) 730
BsaJI CCNNGG 5 cut(s) 102, 140, 2058, 2353, 3381
BsaWI WCCGGW 2 cut(s) 460, 3311
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 9 cut(s) 103, 204, 1067, 1068, 1157, 1777, 2773, 3274, 3278
Bse1I ACTGG 9 cut(s) 164, 899, 1072, 1564, 2471, 2723, 2751, 3124, 3287
Bse21I CCTNAGG 1 cut(s) 3438
Bse3DI GCAATG 1 cut(s) 2997
Bse8I GATNNNNATC 1 cut(s) 1314
BseAI TCCGGA 2 cut(s) 460, 3311
BseBI CCWGG 5 cut(s) 513, 1032, 1246, 3090, 3383
BseDI CCNNGG 5 cut(s) 102, 140, 2058, 2353, 3381
BseGI GGATG 9 cut(s) 469, 676, 1375, 1900, 2434, 2641, 3208, 3268, 3283
BseJI GATNNNNATC 1 cut(s) 1314
BseLI CCNNNNNNNGG 9 cut(s) 103, 204, 1067, 1068, 1157, 1777, 2773, 3274, 3278
BseMI GCAATG 1 cut(s) 2997
BseMII CTCAG 7 cut(s) 100, 269, 1413, 2147, 2203, 2523, 3225
BseNI ACTGG 9 cut(s) 164, 899, 1072, 1564, 2471, 2723, 2751, 3124, 3287
BseRI GAGGAG 1 cut(s) 2573
BseSI GKGCMC 1 cut(s) 343
BseYI CCCAGC 1 cut(s) 198
BsgI GTGCAG 4 cut(s) 864, 1305, 1575, 2973
Bsh1236I CGCG 1 cut(s) 337
BshFI GGCC 6 cut(s) 107, 511, 1201, 1427, 2141, 2703
BsiHKAI GWGCWC 3 cut(s) 1444, 1504, 2870
BsiSI CCGG 5 cut(s) 140, 461, 1572, 2425, 3312
BslFI GGGAC 3 cut(s) 157, 370, 1184
BslI CCNNNNNNNGG 9 cut(s) 103, 204, 1067, 1068, 1157, 1777, 2773, 3274, 3278
BsmAI GTCTC 5 cut(s) 456, 730, 887, 2680, 2795
BsmFI GGGAC 3 cut(s) 157, 370, 1184
BsmI GAATGC 2 cut(s) 1705, 2134
BsnI GGCC 6 cut(s) 107, 511, 1201, 1427, 2141, 2703
Bso31I GGTCTC 1 cut(s) 730
Bsp1286I GDGCHC 5 cut(s) 343, 494, 1444, 1504, 2870
Bsp13I TCCGGA 2 cut(s) 460, 3311
Bsp1407I TGTACA 2 cut(s) 2223, 3139
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 6 cut(s) 94, 335, 1062, 1198, 1849, 1899
BspANI GGCC 6 cut(s) 107, 511, 1201, 1427, 2141, 2703
BspCNI CTCAG 7 cut(s) 99, 270, 1414, 2146, 2202, 2524, 3224
BspEI TCCGGA 2 cut(s) 460, 3311
BspFNI CGCG 1 cut(s) 337
BspHI TCATGA 1 cut(s) 1411
BspLI GGNNCC 8 cut(s) 493, 1452, 2265, 2392, 3066, 3094, 3266, 3366
BspMAI CTGCAG 2 cut(s) 117, 1449
BspMI ACCTGC 1 cut(s) 640
BspPI GGATC 5 cut(s) 691, 1198, 1304, 2426, 3139
BspTI CTTAAG 3 cut(s) 328, 1256, 1276
BspTNI GGTCTC 1 cut(s) 730
BsrDI GCAATG 1 cut(s) 2997
BsrGI TGTACA 2 cut(s) 2223, 3139
BsrI ACTGG 9 cut(s) 164, 899, 1072, 1564, 2471, 2723, 2751, 3124, 3287
BssECI CCNNGG 5 cut(s) 102, 140, 2058, 2353, 3381
BssT1I CCWWGG 3 cut(s) 102, 2058, 2353
Bst2UI CCWGG 5 cut(s) 513, 1032, 1246, 3090, 3383
Bst4CI ACNGT 8 cut(s) 248, 601, 1078, 1536, 2248, 2623, 2872, 3220
Bst6I CTCTTC 1 cut(s) 52
BstAFI CTTAAG 3 cut(s) 328, 1256, 1276
BstAPI GCANNNNNTGC 3 cut(s) 806, 878, 1499
BstAUI TGTACA 2 cut(s) 2223, 3139
BstBAI YACGTR 1 cut(s) 1379
BstDSI CCRYGG 1 cut(s) 102
BstF5I GGATG 9 cut(s) 469, 676, 1375, 1900, 2434, 2641, 3208, 3268, 3283
BstFNI CGCG 1 cut(s) 337
BstHHI GCGC 2 cut(s) 1720, 1795
BstMAI GTCTC 5 cut(s) 456, 730, 887, 2680, 2795
BstNI CCWGG 5 cut(s) 513, 1032, 1246, 3090, 3383
BstNSI RCATGY 3 cut(s) 2256, 2337, 2843
BstSCI CCNGG 8 cut(s) 139, 511, 1030, 1244, 1571, 2424, 3088, 3381
BstSFI CTRYAG 7 cut(s) 113, 244, 397, 795, 1445, 3186, 3474
BstSLI GKGCMC 1 cut(s) 343
BstSNI TACGTA 1 cut(s) 1379
BstUI CGCG 1 cut(s) 337
BstV2I GAAGAC 4 cut(s) 2581, 3036, 3122, 3159
BstX2I RGATCY 2 cut(s) 1190, 1309
BstXI CCANNNNNNTGG 1 cut(s) 1747
BstYI RGATCY 2 cut(s) 1190, 1309
Bsu36I CCTNAGG 1 cut(s) 3438
BsuI GTATCC 2 cut(s) 2190, 3158
BsuRI GGCC 6 cut(s) 107, 511, 1201, 1427, 2141, 2703
BtgI CCRYGG 1 cut(s) 102
BtrI CACGTC 2 cut(s) 240, 2380
BtsCI GGATG 9 cut(s) 469, 676, 1375, 1900, 2434, 2641, 3208, 3268, 3283
BtsI GCAGTG 1 cut(s) 122
BveI ACCTGC 1 cut(s) 640
CaiI CAGNNNCTG 3 cut(s) 806, 1193, 1313
CciI TCATGA 1 cut(s) 1411
CfoI GCGC 2 cut(s) 1720, 1795
Cfr13I GGNCC 7 cut(s) 18, 1200, 1477, 1750, 2140, 3122, 3309
CseI GACGC 2 cut(s) 3342, 3398
Csp6I GTAC 6 cut(s) 831, 1057, 2224, 2748, 2995, 3140
CviQI GTAC 6 cut(s) 831, 1057, 2224, 2748, 2995, 3140
DraI TTTAAA 4 cut(s) 630, 1636, 1819, 3297
DraIII CACNNNGTG 1 cut(s) 1499
DrdI GACNNNNNNGTC 1 cut(s) 151
DseDI GACNNNNNNGTC 1 cut(s) 151
EaeI YGGCCR 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
EciI GGCGGA 1 cut(s) 83
Eco105I TACGTA 1 cut(s) 1379
Eco130I CCWWGG 3 cut(s) 102, 2058, 2353
Eco147I AGGCCT 3 cut(s) 511, 1427, 2703
Eco24I GRGCYC 1 cut(s) 494
Eco31I GGTCTC 1 cut(s) 730
Eco32I GATATC 2 cut(s) 2360, 3248
Eco47I GGWCC 5 cut(s) 18, 1477, 1750, 3122, 3309
Eco57I CTGAAG 5 cut(s) 63, 612, 678, 1871, 2477
Eco81I CCTNAGG 1 cut(s) 3438
EcoO109I RGGNCCY 1 cut(s) 18
EcoRII CCWGG 5 cut(s) 511, 1030, 1244, 3088, 3381
EcoRV GATATC 2 cut(s) 2360, 3248
EcoT14I CCWWGG 3 cut(s) 102, 2058, 2353
EcoT38I GRGCYC 1 cut(s) 494
ErhI CCWWGG 3 cut(s) 102, 2058, 2353
FalI AAGNNNNNCTT 2 cut(s) 1259, 1291
FaqI GGGAC 3 cut(s) 157, 370, 1184
FauI CCCGC 1 cut(s) 1069
FauNDI CATATG 1 cut(s) 2587
FbaI TGATCA 3 cut(s) 234, 1465, 1995
FblI GTMKAC 2 cut(s) 903, 3433
FokI GGATG 9 cut(s) 476, 683, 1382, 1907, 2441, 2628, 3215, 3255, 3290
FriOI GRGCYC 1 cut(s) 494
FspBI CTAG 4 cut(s) 420, 1152, 1182, 1595
FspI TGCGCA 1 cut(s) 1794
GlaI GCGC 2 cut(s) 1719, 1794
GsaI CCCAGC 1 cut(s) 202
GsuI CTGGAG 5 cut(s) 147, 882, 2409, 3254, 3404
HaeIII GGCC 6 cut(s) 107, 511, 1201, 1427, 2141, 2703
HapII CCGG 5 cut(s) 140, 461, 1572, 2425, 3312
HgaI GACGC 2 cut(s) 3342, 3398
HhaI GCGC 2 cut(s) 1720, 1795
Hin6I GCGC 2 cut(s) 1718, 1793
HinP1I GCGC 2 cut(s) 1718, 1793
HincII GTYRAC 1 cut(s) 3434
HindII GTYRAC 1 cut(s) 3434
HindIII AAGCTT 5 cut(s) 677, 1812, 1937, 1949, 2433
HpaII CCGG 5 cut(s) 140, 461, 1572, 2425, 3312
Hpy166II GTNNAC 4 cut(s) 904, 2226, 3208, 3434
Hpy8I GTNNAC 4 cut(s) 904, 2226, 3208, 3434
Hpy99I CGWCG 1 cut(s) 3336
HpyCH4III ACNGT 8 cut(s) 248, 601, 1078, 1536, 2248, 2623, 2872, 3220
HpyCH4IV ACGT 4 cut(s) 239, 304, 1378, 2379
HpySE526I ACGT 4 cut(s) 239, 304, 1378, 2379
HspAI GCGC 2 cut(s) 1718, 1793
Kpn2I TCCGGA 2 cut(s) 460, 3311
Ksp22I TGATCA 3 cut(s) 234, 1465, 1995
LmnI GCTCC 9 cut(s) 40, 277, 497, 869, 1456, 2060, 2263, 2390, 2560
LweI GCATC 9 cut(s) 618, 1313, 1344, 1360, 1885, 2099, 2485, 2546, 3231
MaeI CTAG 4 cut(s) 420, 1152, 1182, 1595
MaeII ACGT 4 cut(s) 239, 304, 1378, 2379
MaeIII GTNAC 6 cut(s) 466, 984, 1096, 2758, 3214, 3253
MfeI CAATTG 1 cut(s) 2274
MflI RGATCY 2 cut(s) 1190, 1309
MhlI GDGCHC 5 cut(s) 343, 494, 1444, 1504, 2870
MlsI TGGCCA 1 cut(s) 107
MluNI TGGCCA 1 cut(s) 107
MlyI GAGTC 9 cut(s) 84, 382, 889, 1100, 1243, 1587, 2321, 2716, 2950
MmeI TCCRAC 4 cut(s) 406, 634, 1728, 2222
Mox20I TGGCCA 1 cut(s) 107
MroI TCCGGA 2 cut(s) 460, 3311
MroXI GAANNNNTTC 4 cut(s) 193, 2049, 2578, 3105
MscI TGGCCA 1 cut(s) 107
MslI CAYNNNNRTG 2 cut(s) 1917, 2481
Msp20I TGGCCA 1 cut(s) 107
MspA1I CMGCKG 3 cut(s) 977, 1054, 2910
MspCI CTTAAG 3 cut(s) 328, 1256, 1276
MspI CCGG 5 cut(s) 140, 461, 1572, 2425, 3312
MspR9I CCNGG 8 cut(s) 141, 513, 1032, 1246, 1573, 2426, 3090, 3383
MunI CAATTG 1 cut(s) 2274
Mva1269I GAATGC 2 cut(s) 1705, 2134
MvaI CCWGG 5 cut(s) 513, 1032, 1246, 3090, 3383
MvnI CGCG 1 cut(s) 337
NciI CCSGG 3 cut(s) 141, 1573, 2426
NcoI CCATGG 1 cut(s) 102
NdeI CATATG 1 cut(s) 2587
NlaIV GGNNCC 8 cut(s) 493, 1452, 2265, 2392, 3066, 3094, 3266, 3366
NmuCI GTSAC 4 cut(s) 466, 984, 2758, 3253
NsbI TGCGCA 1 cut(s) 1794
NspI RCATGY 3 cut(s) 2256, 2337, 2843
PaeI GCATGC 1 cut(s) 2256
PagI TCATGA 1 cut(s) 1411
PceI AGGCCT 3 cut(s) 511, 1427, 2703
PciI ACATGT 1 cut(s) 2839
PctI GAATGC 2 cut(s) 1705, 2134
PdmI GAANNNNTTC 4 cut(s) 193, 2049, 2578, 3105
PflMI CCANNNNNTGG 1 cut(s) 2773
PfoI TCCNGGA 1 cut(s) 1030
PleI GAGTC 9 cut(s) 84, 381, 889, 1099, 1243, 1586, 2320, 2716, 2949
PpsI GAGTC 9 cut(s) 84, 381, 889, 1099, 1243, 1586, 2320, 2716, 2949
Ppu21I YACGTR 1 cut(s) 1379
PpuMI RGGWCCY 1 cut(s) 18
PscI ACATGT 1 cut(s) 2839
PshBI ATTAAT 1 cut(s) 1803
PsiI TTATAA 1 cut(s) 2889
Psp5II RGGWCCY 1 cut(s) 18
Psp6I CCWGG 5 cut(s) 511, 1030, 1244, 3088, 3381
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 5 cut(s) 511, 1030, 1244, 3088, 3381
PspN4I GGNNCC 8 cut(s) 493, 1452, 2265, 2392, 3066, 3094, 3266, 3366
PspPI GGNCC 7 cut(s) 18, 1200, 1477, 1750, 2140, 3122, 3309
PspPPI RGGWCCY 1 cut(s) 18
PsrI GAACNNNNNNTAC 2 cut(s) 2034, 2066
PstI CTGCAG 2 cut(s) 117, 1449
PstNI CAGNNNCTG 3 cut(s) 806, 1193, 1313
PsuI RGATCY 2 cut(s) 1190, 1309
PvuII CAGCTG 3 cut(s) 977, 1054, 2910
RsaI GTAC 6 cut(s) 832, 1058, 2225, 2749, 2996, 3141
RsaNI GTAC 6 cut(s) 831, 1057, 2224, 2748, 2995, 3140
RseI CAYNNNNRTG 2 cut(s) 1917, 2481
SalI GTCGAC 1 cut(s) 3432
Sau96I GGNCC 7 cut(s) 18, 1200, 1477, 1750, 2140, 3122, 3309
ScaI AGTACT 1 cut(s) 832
SchI GAGTC 9 cut(s) 84, 382, 889, 1100, 1243, 1587, 2321, 2716, 2950
ScrFI CCNGG 8 cut(s) 141, 513, 1032, 1246, 1573, 2426, 3090, 3383
SduI GDGCHC 5 cut(s) 343, 494, 1444, 1504, 2870
SfaNI GCATC 9 cut(s) 618, 1313, 1344, 1360, 1885, 2099, 2485, 2546, 3231
SfcI CTRYAG 7 cut(s) 113, 244, 397, 795, 1445, 3186, 3474
SinI GGWCC 5 cut(s) 18, 1477, 1750, 3122, 3309
SmiMI CAYNNNNRTG 2 cut(s) 1917, 2481
SmlI CTYRAG 6 cut(s) 328, 453, 1256, 1276, 2495, 2863
SmoI CTYRAG 6 cut(s) 328, 453, 1256, 1276, 2495, 2863
SnaBI TACGTA 1 cut(s) 1379
SphI GCATGC 1 cut(s) 2256
SseBI AGGCCT 3 cut(s) 511, 1427, 2703
SsiI CCGC 6 cut(s) 94, 335, 1062, 1198, 1849, 1899
SspI AATATT 2 cut(s) 1528, 1649
SspMI CTAG 4 cut(s) 420, 1152, 1182, 1595
StuI AGGCCT 3 cut(s) 511, 1427, 2703
StyD4I CCNGG 8 cut(s) 139, 511, 1030, 1244, 1571, 2424, 3088, 3381
StyI CCWWGG 3 cut(s) 102, 2058, 2353
TaaI ACNGT 8 cut(s) 248, 601, 1078, 1536, 2248, 2623, 2872, 3220
TaiI ACGT 4 cut(s) 242, 307, 1381, 2382
TaqI TCGA 9 cut(s) 404, 605, 752, 812, 1626, 2725, 2944, 3331, 3433
TatI WGTACW 4 cut(s) 830, 2223, 2994, 3139
TauI GCSGC 2 cut(s) 1201, 1901
TseFI GTSAC 4 cut(s) 466, 984, 2758, 3253
Tsp45I GTSAC 4 cut(s) 466, 984, 2758, 3253
TspGWI ACGGA 1 cut(s) 3436
Van91I CCANNNNNTGG 1 cut(s) 2773
Vha464I CTTAAG 3 cut(s) 328, 1256, 1276
VpaK11BI GGWCC 5 cut(s) 18, 1477, 1750, 3122, 3309
VspI ATTAAT 1 cut(s) 1803
XapI RAATTY 3 cut(s) 970, 1471, 2794
XbaI TCTAGA 1 cut(s) 419
XceI RCATGY 3 cut(s) 2256, 2337, 2843
XcmI CCANNNNNNNNNTGG 3 cut(s) 1242, 1647, 3275
XmiI GTMKAC 2 cut(s) 903, 3433
XmnI GAANNNNTTC 4 cut(s) 193, 2049, 2578, 3105
XspI CTAG 4 cut(s) 420, 1152, 1182, 1595
ZrmI AGTACT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.