RchiOBHm_Chr6g0305241

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006256
Physical Location & Seq
Forward (+)
64289638 .. 64301789
12152 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27431

Sequence Viewer

Length: 3510 bp
ATGATTCATTTCCACAGGACCTTCTCATTGACAGCTCCTTCTTCCTCTTCATTCATGTTCTCATACCCTTCAACTTCTTCAGCTCTCAGACTCCGCCTCTCCATGGCCAAGCTGCAGTGTAGGGCGTTTGAGCATTATCCGGGCACTTGCTTGCCGTCTTCTCCAGTCTTGACATTGAATAATTACCGAAACTATTCCCAGCTTAGGTTTAGAAAATCCAGAGTTTCTTGCATGATCACGTCCTACAGTGCGAAAGTTGGGCCGACGTTTGCTCCTTTGAGAAGAAGTGGTGATGGGTTTCAACGTGCTTTTAGCATTGATTCACGCTTAAGACCCGGTGCCCTTAATGCTTTGGGGACTGCTAGTGTTATGAGTCGTGGGTTTTTTGGAAATGGCTGTAGATCAAGTGTATTGGATTCTAGAGTTGGAGATTTTTCCAAGAGGTTTTTGTCTCAAGTTCCGGACGTGACAAGTAAAGTAGAGAATGTGGTCTCCCAAGATGGCAGGAGGCCTGGAAATAGAAAATGGTTCAAAATAATGATTAGCAATCGCAAGAAATTGAAAAAGGCATTGGCTACTCAAAGTGAAACTGAAGCTACCGTTTCGAGCATCTCCAAAGATGCTGATGTAAAAGTTTCAAATGAAAAGCAGGTTGGAAGTGAGAAGGAGGATGAGAAGCTTGGATCATCAACTTCAGATTCGCCTATAAGCGAAACTAAAGAGGTCTCTAAACCAAAAGCCAAAAAGAGGTCGAAAAGCAAGAAAACTAAAGAACAAGATTCTGCTGCTAATGCTATAGCAGAAGCTGCTTCGACACAAAGTTCTGAAAGTACTTCTCAAACAAAGAAATCTGGCAGGAACAAAAAAGGAGCAAAGTCTGCACAGGATTCAGAGACTCCAGTAGACCTTGCTGATAGTTCAACAACAAAATCCCAGAGAAACAAGAAAGGTGAGAAGGAAAAATCTATAAATTCAGCTGCCAAGTCACCACAGAAACTTATACCACAAGAAATGGGAAAATTAAAACCTCCTGGAAAAATGACAACGAAGCAGCTGTACCCCGCAACTGGGAAAACTGTTGTGGTGGTGGAGTCTGTTACAAAGGCGAAGGTTATTCAGGGTTATCTTGGTGATATGTTTGAAGTGCTGCCTAGCTATGGTCATGTGAGGGACTTGGCAGCTAGGTCAGGATCTGTGCGGCCCGATGATGATTTTAGTATGGTGTGGGAAGTTCCATCTGCTGCCTGGACTCATCTTAAGAGCATAAAGGTCGCCCTAAGTGGTGCGGAAAATCTTATCCTTGCATCAGATCCTGATCGTGAAGGAGAGGCTATTGCTTGGCATATTATTGAGATGCTGCAACAACAGGATGCTTTACGTAAAGATATTTATGTAGCAAGGGTTGTCTTTCATGAAATCACTGAGGCCTCCATCAAAAGTGCGCTGCAGGCTCCCAGAGAAATTGATCAAAATTTGGTCCATGCTTACCTTGCACGGCGTGCTCTTGATTATTTGATCGGATTTAATATTTCACCACTGCTATGGAGGAAACTGCCAAGTTGCCAGTCTGCCGGGCGAGTCCAATCTGCTGCACTAGCTCTTGTATGCGACAGGGAAATGGAAATCGACAGATTTAAACCACAAGAATATTGGACTATTGATATTGAATTGAACAAAAAAGAACCAGATTCATCAGTGAATGCCATATCCTTGCCAGCGCACTTGACCCATTTTGATTCCAAAAAGTTGGACCAATTTTCTATCAGTTCTCATACAGAGGCAAAAGATATTGAGCAGCAAATTAATTCAGAAGCTTTTAAAGTTATCAACTCTAAGAGAAACAAAATGCGGAAGAACCCTCCTACGCCTTATATAACATCAACACTTCAGCAGGATGCCGCAAACAAATTACATTTCAGTGCAACATATACAATGAAGCTTGCACAGAAGCTTTATGAGGGGGTTCAATTATCTGATGGCAAGTCAACGGGTTTGATCACATACACCAGAACAGATGGACTACACATTTCGGATGAAGCAGTAGAAGGTGTTCGCTCCTTGGTCATCGAAAGGTATGGACATAACTTTGCATCAGCTACTCTACGCAAGTTTTTTAAGAAGGTGAAGAATGCTCAGGAGGCCCATGAAGCAATCAGACCTACTGATGTTCAAAGATTTCCATCGTTGCTCAGAGGGATACTTGATGAAGATTCATTGAAGTTGTACACTCTTATTTGGTGTCGGACAGTTGCATGCCAAATGGAGCCTGCTACAATTGAGCAGATACAACTTGATATTGGAAATGCTGGTGAGTCCATTGTTTTCCGATCTGCATGCTCTAGAGTTCAGTTCCTTGGATATCAGGCAGTTTTTCAGGACATGGAAGCTGGAGCCGTCAAATATAAAGAAAATGAAGGGATCAACCGGGATGAAGCTTTTGCACTTCTTGATTCACTGAAGGTTGGCAGTCCAGTGCATCTTGGTGAAGTGGAACTCAAGCAACACCATACCCAACCACCTCCACGCTACTCTGAGGCATCATTGGTTAAGAAATTAGAGGAGCTTGGGATTGGAAGACCTTCAACATATGCAAGCACTATAAAGGTTTTACAGGACAGAAACTATCTGACAGTGAAAAGCCGTGTTTTACATCCAGAGTTTCGTGGTCGTATGGTGTCAGCATTTCTCTGCCACCATTTTTCTGAGGTCACAGATTATAGCTTCACAGCTGATATGGAGACCGAGCTTGATAATGTCTCTGCTGGCTTAACTGAATGGAAAGGCCTCCTAAGAGATTACTGGACTCGATTCAGTGGTTATTGTGAGCGTACCAGTAATGTGACTATCCATCAGGTGGAGAAAATGATGGAAAAAAAATTTGGAGACTTCTTGTTTACTACTCTCCCTGATCAAAGCAGGACATGTCCAAGCTGTAATGAAGGAACTTTGATCTTCAAAGTGAGTCGATTCGGTGCAGGCTATTTCATAGGTTGTGATACACACCCAAAGTGCAAGTACATTGCTAAGACATTGTATGGTAATGAAGAAGAAGACGACAATACTCCGAAGAACAACAGGGTGGAGGAACCAAAATTGTTAGGTTTATCTCCCGGTTCCAATGAAAAGGTTCTTTTGAAGACTGGTCCTTATGGATCGTATGTACAACTTGGTGAAGACAGGAAGGGATACTCACCTAAAAGAGCCTCTATAGCACATATCAAGGATGTGAACTCAGTAACCGTTGAAGTTGCTCTCGAGTTGATGCGATATCCTGTGACATTGGGGAACCATCCGAAGGATGGGCTACCAGTGATTTTAAAGATTGCAAGGTCCGGATATATAGTTAAACATCGACGCACAATGGCTTCTCTAACAAAGAATCAGGAACCTAATAAATTAACCCTGGAGGATGCACTGGAACTTTTGTCTGGGAAAAATACACGGAAAATTGGTCGACCTAAGGGAAAATCAAAGGTTGAAGAAAAAGGAGAAGCTATAGAAGCCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topoisom_bac Toprim Toprim_C_rpt zf-C4_Topoisom

Protein Analysis

1169

Amino Acids

130.34

Weight (kDa)

9.34

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Toprim PF01751 359 - 472 1.9e-16 Toprim domain
Topoisom_bac PF01131 488 - 929 1.4e-111 DNA topoisomerase
Zn_ribbon_Top1 PF01396 973 - 1009 6.7e-09 Topoisomerase DNA binding C4 zinc finger
Toprim_C_rpt PF13368 1037 - 1091 1e-08 Topoisomerase C-terminal repeat
Toprim_C_rpt PF13368 1097 - 1148 1.1e-07 Topoisomerase C-terminal repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 640
AccB1I GGYRCC 1 cut(s) 338
AccB7I CCANNNNNTGG 1 cut(s) 2854
AccI GTMKAC 2 cut(s) 903, 3454
AccIII TCCGGA 2 cut(s) 460, 3332
AciI CCGC 6 cut(s) 94, 1062, 1198, 1286, 1849, 1899
AclWI GGATC 5 cut(s) 691, 1198, 1304, 2426, 3160
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 3 cut(s) 970, 1471, 2876
AcuI CTGAAG 5 cut(s) 63, 612, 678, 1871, 2477
AdeI CACNNNGTG 1 cut(s) 1499
AfaI GTAC 6 cut(s) 832, 1058, 2225, 2830, 3017, 3162
AfiI CCNNNNNNNGG 9 cut(s) 103, 204, 747, 1067, 1068, 1157, 2854, 3295, 3299
AflII CTTAAG 2 cut(s) 328, 1256
AflIII ACRYGT 1 cut(s) 2921
AjiI CACGTC 2 cut(s) 240, 466
AjnI CCWGG 4 cut(s) 511, 1030, 1244, 3402
AjuI GAANNNNNNNTTGG 6 cut(s) 554, 586, 636, 668, 2862, 2894
Alw21I GWGCWC 1 cut(s) 1504
Alw26I GTCTC 7 cut(s) 456, 496, 730, 887, 2732, 2761, 2877
AlwI GGATC 5 cut(s) 691, 1198, 1304, 2426, 3160
AlwNI CAGNNNCTG 3 cut(s) 806, 1193, 1313
Ama87I CYCGRG 1 cut(s) 3254
Aor13HI TCCGGA 2 cut(s) 460, 3332
AoxI GGCC 7 cut(s) 105, 260, 509, 1199, 1425, 2139, 2782
ApoI RAATTY 3 cut(s) 970, 1471, 2876
AseI ATTAAT 1 cut(s) 1803
Asp700I GAANNNNTTC 4 cut(s) 193, 2049, 2578, 3126
AspLEI GCGC 2 cut(s) 1444, 1720
AspS9I GGNCC 8 cut(s) 18, 260, 1200, 1477, 1750, 2140, 3143, 3330
AsuC2I CCSGG 5 cut(s) 141, 336, 1573, 2426, 3111
AvaI CYCGRG 1 cut(s) 3254
AvaII GGWCC 5 cut(s) 18, 1477, 1750, 3143, 3330
AxyI CCTNAGG 1 cut(s) 3459
BaeGI GKGCMC 2 cut(s) 146, 343
BalI TGGCCA 1 cut(s) 107
BanI GGYRCC 1 cut(s) 338
BarI GAAGNNNNNNTAC 2 cut(s) 1040, 1072
BbsI GAAGAC 5 cut(s) 150, 2581, 3057, 3143, 3180
Bbv12I GWGCWC 1 cut(s) 1504
BceAI ACGGC 4 cut(s) 139, 1511, 2378, 2625
BcgI CGANNNNNNTGC 4 cut(s) 1252, 1286, 2316, 2350
BciT130I CCWGG 4 cut(s) 513, 1032, 1246, 3404
BciVI GTATCC 2 cut(s) 2190, 3179
BclI TGATCA 4 cut(s) 234, 1465, 1995, 2908
BcnI CCSGG 5 cut(s) 141, 336, 1573, 2426, 3111
BcoDI GTCTC 7 cut(s) 456, 496, 730, 887, 2732, 2761, 2877
BfaI CTAG 6 cut(s) 363, 420, 1152, 1182, 1595, 2340
BfmI CTRYAG 7 cut(s) 113, 244, 397, 795, 1445, 3207, 3495
BfrI CTTAAG 2 cut(s) 328, 1256
BfuAI ACCTGC 1 cut(s) 640
BfuI GTATCC 2 cut(s) 2190, 3179
BmcAI AGTACT 1 cut(s) 832
Bme1390I CCNGG 9 cut(s) 141, 336, 513, 1032, 1246, 1573, 2426, 3111, 3404
Bme18I GGWCC 5 cut(s) 18, 1477, 1750, 3143, 3330
BmeT110I CYCGRG 1 cut(s) 3254
BmgBI CACGTC 2 cut(s) 240, 466
BmgT120I GGNCC 8 cut(s) 18, 260, 1200, 1477, 1750, 2140, 3143, 3330
BmiI GGNNCC 8 cut(s) 340, 1452, 2265, 2392, 3087, 3115, 3287, 3387
BmrFI CCNGG 9 cut(s) 141, 336, 513, 1032, 1246, 1573, 2426, 3111, 3404
BmrI ACTGGG 1 cut(s) 1077
BmuI ACTGGG 1 cut(s) 1077
BpiI GAAGAC 5 cut(s) 150, 2581, 3057, 3143, 3180
BpmI CTGGAG 4 cut(s) 147, 882, 2409, 3425
Bpu10I CCTNAGC 2 cut(s) 203, 2133
BpuEI CTTGAG 2 cut(s) 438, 2480
BpuMI CCSGG 5 cut(s) 141, 336, 1573, 2426, 3111
BsaAI YACGTR 1 cut(s) 1379
BsaBI GATNNNNATC 2 cut(s) 1314, 2179
BsaI GGTCTC 3 cut(s) 496, 730, 2732
BsaJI CCNNGG 4 cut(s) 102, 2058, 2353, 3402
BsaWI WCCGGW 2 cut(s) 460, 3332
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 9 cut(s) 103, 204, 747, 1067, 1068, 1157, 2854, 3295, 3299
Bse21I CCTNAGG 1 cut(s) 3459
Bse3DI GCAATG 1 cut(s) 3018
Bse8I GATNNNNATC 2 cut(s) 1314, 2179
BseAI TCCGGA 2 cut(s) 460, 3332
BseBI CCWGG 4 cut(s) 513, 1032, 1246, 3404
BseDI CCNNGG 4 cut(s) 102, 2058, 2353, 3402
BseJI GATNNNNATC 2 cut(s) 1314, 2179
BseLI CCNNNNNNNGG 9 cut(s) 103, 204, 747, 1067, 1068, 1157, 2854, 3295, 3299
BseMI GCAATG 1 cut(s) 3018
BseMII CTCAG 7 cut(s) 100, 1413, 2147, 2203, 2523, 2694, 3246
BseRI GAGGAG 1 cut(s) 2573
BseSI GKGCMC 2 cut(s) 146, 343
BseYI CCCAGC 1 cut(s) 198
BsgI GTGCAG 3 cut(s) 864, 1575, 2994
BshFI GGCC 7 cut(s) 107, 262, 511, 1201, 1427, 2141, 2784
BshNI GGYRCC 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 1504
BsiHKCI CYCGRG 1 cut(s) 3254
BsiSI CCGG 7 cut(s) 140, 336, 461, 1572, 2425, 3111, 3333
BslFI GGGAC 2 cut(s) 370, 1184
BslI CCNNNNNNNGG 9 cut(s) 103, 204, 747, 1067, 1068, 1157, 2854, 3295, 3299
BsmAI GTCTC 7 cut(s) 456, 496, 730, 887, 2732, 2761, 2877
BsmFI GGGAC 2 cut(s) 370, 1184
BsmI GAATGC 2 cut(s) 1705, 2134
BsnI GGCC 7 cut(s) 107, 262, 511, 1201, 1427, 2141, 2784
Bso31I GGTCTC 3 cut(s) 496, 730, 2732
BsoBI CYCGRG 1 cut(s) 3254
Bsp1286I GDGCHC 3 cut(s) 146, 343, 1504
Bsp13I TCCGGA 2 cut(s) 460, 3332
Bsp1407I TGTACA 2 cut(s) 2223, 3160
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 6 cut(s) 94, 1062, 1198, 1286, 1849, 1899
BspANI GGCC 7 cut(s) 107, 262, 511, 1201, 1427, 2141, 2784
BspCNI CTCAG 7 cut(s) 99, 1414, 2146, 2202, 2524, 2695, 3245
BspEI TCCGGA 2 cut(s) 460, 3332
BspHI TCATGA 1 cut(s) 1411
BspLI GGNNCC 8 cut(s) 340, 1452, 2265, 2392, 3087, 3115, 3287, 3387
BspMAI CTGCAG 2 cut(s) 117, 1449
BspMI ACCTGC 1 cut(s) 640
BspPI GGATC 5 cut(s) 691, 1198, 1304, 2426, 3160
BspT107I GGYRCC 1 cut(s) 338
BspTI CTTAAG 2 cut(s) 328, 1256
BspTNI GGTCTC 3 cut(s) 496, 730, 2732
BsrDI GCAATG 1 cut(s) 3018
BsrGI TGTACA 2 cut(s) 2223, 3160
BssECI CCNNGG 4 cut(s) 102, 2058, 2353, 3402
BssT1I CCWWGG 3 cut(s) 102, 2058, 2353
Bst2UI CCWGG 4 cut(s) 513, 1032, 1246, 3404
Bst4CI ACNGT 6 cut(s) 248, 601, 1078, 2248, 2632, 3241
Bst6I CTCTTC 1 cut(s) 52
BstAFI CTTAAG 2 cut(s) 328, 1256
BstAPI GCANNNNNTGC 3 cut(s) 806, 878, 1499
BstAUI TGTACA 2 cut(s) 2223, 3160
BstBAI YACGTR 1 cut(s) 1379
BstDSI CCRYGG 1 cut(s) 102
BstHHI GCGC 2 cut(s) 1444, 1720
BstMAI GTCTC 7 cut(s) 456, 496, 730, 887, 2732, 2761, 2877
BstNI CCWGG 4 cut(s) 513, 1032, 1246, 3404
BstNSI RCATGY 3 cut(s) 2256, 2337, 2925
BstSCI CCNGG 9 cut(s) 139, 334, 511, 1030, 1244, 1571, 2424, 3109, 3402
BstSFI CTRYAG 7 cut(s) 113, 244, 397, 795, 1445, 3207, 3495
BstSLI GKGCMC 2 cut(s) 146, 343
BstSNI TACGTA 1 cut(s) 1379
BstV2I GAAGAC 5 cut(s) 150, 2581, 3057, 3143, 3180
BstX2I RGATCY 2 cut(s) 1190, 1309
BstXI CCANNNNNNTGG 2 cut(s) 1542, 1747
BstYI RGATCY 2 cut(s) 1190, 1309
Bsu36I CCTNAGG 1 cut(s) 3459
BsuI GTATCC 2 cut(s) 2190, 3179
BsuRI GGCC 7 cut(s) 107, 262, 511, 1201, 1427, 2141, 2784
BtgI CCRYGG 1 cut(s) 102
BtrI CACGTC 2 cut(s) 240, 466
BtsI GCAGTG 2 cut(s) 122, 1535
BveI ACCTGC 1 cut(s) 640
CaiI CAGNNNCTG 3 cut(s) 806, 1193, 1313
CciI TCATGA 1 cut(s) 1411
CfoI GCGC 2 cut(s) 1444, 1720
Cfr13I GGNCC 8 cut(s) 18, 260, 1200, 1477, 1750, 2140, 3143, 3330
CseI GACGC 1 cut(s) 3363
Csp6I GTAC 6 cut(s) 831, 1057, 2224, 2829, 3016, 3161
CviQI GTAC 6 cut(s) 831, 1057, 2224, 2829, 3016, 3161
DraI TTTAAA 3 cut(s) 1636, 1819, 3318
DraIII CACNNNGTG 1 cut(s) 1499
EaeI YGGCCR 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
EciI GGCGGA 1 cut(s) 83
Eco105I TACGTA 1 cut(s) 1379
Eco130I CCWWGG 3 cut(s) 102, 2058, 2353
Eco147I AGGCCT 3 cut(s) 511, 1427, 2784
Eco31I GGTCTC 3 cut(s) 496, 730, 2732
Eco32I GATATC 2 cut(s) 2360, 3269
Eco47I GGWCC 5 cut(s) 18, 1477, 1750, 3143, 3330
Eco57I CTGAAG 5 cut(s) 63, 612, 678, 1871, 2477
Eco81I CCTNAGG 1 cut(s) 3459
Eco88I CYCGRG 1 cut(s) 3254
EcoO109I RGGNCCY 1 cut(s) 18
EcoRII CCWGG 4 cut(s) 511, 1030, 1244, 3402
EcoRV GATATC 2 cut(s) 2360, 3269
EcoT14I CCWWGG 3 cut(s) 102, 2058, 2353
ErhI CCWWGG 3 cut(s) 102, 2058, 2353
FaqI GGGAC 2 cut(s) 370, 1184
FauI CCCGC 1 cut(s) 1069
FauNDI CATATG 1 cut(s) 2587
FbaI TGATCA 4 cut(s) 234, 1465, 1995, 2908
FblI GTMKAC 2 cut(s) 903, 3454
FspBI CTAG 6 cut(s) 363, 420, 1152, 1182, 1595, 2340
GlaI GCGC 2 cut(s) 1443, 1719
GsaI CCCAGC 1 cut(s) 202
GsuI CTGGAG 4 cut(s) 147, 882, 2409, 3425
HaeIII GGCC 7 cut(s) 107, 262, 511, 1201, 1427, 2141, 2784
HapII CCGG 7 cut(s) 140, 336, 461, 1572, 2425, 3111, 3333
HgaI GACGC 1 cut(s) 3363
HhaI GCGC 2 cut(s) 1444, 1720
Hin6I GCGC 2 cut(s) 1442, 1718
HinP1I GCGC 2 cut(s) 1442, 1718
HincII GTYRAC 2 cut(s) 1986, 3455
HindII GTYRAC 2 cut(s) 1986, 3455
HindIII AAGCTT 5 cut(s) 677, 1812, 1937, 1949, 2433
HpaII CCGG 7 cut(s) 140, 336, 461, 1572, 2425, 3111, 3333
Hpy166II GTNNAC 6 cut(s) 904, 1986, 2226, 2895, 3229, 3455
Hpy8I GTNNAC 6 cut(s) 904, 1986, 2226, 2895, 3229, 3455
Hpy99I CGWCG 2 cut(s) 268, 3357
HpyCH4III ACNGT 6 cut(s) 248, 601, 1078, 2248, 2632, 3241
HpyCH4IV ACGT 5 cut(s) 239, 266, 304, 465, 1378
HpySE526I ACGT 5 cut(s) 239, 266, 304, 465, 1378
HspAI GCGC 2 cut(s) 1442, 1718
Kpn2I TCCGGA 2 cut(s) 460, 3332
Ksp22I TGATCA 4 cut(s) 234, 1465, 1995, 2908
LmnI GCTCC 8 cut(s) 40, 277, 869, 1456, 2060, 2263, 2390, 2560
MaeI CTAG 6 cut(s) 363, 420, 1152, 1182, 1595, 2340
MaeII ACGT 5 cut(s) 239, 266, 304, 465, 1378
MaeIII GTNAC 7 cut(s) 466, 984, 1096, 2707, 2839, 3235, 3274
MfeI CAATTG 1 cut(s) 2274
MflI RGATCY 2 cut(s) 1190, 1309
MhlI GDGCHC 3 cut(s) 146, 343, 1504
MlsI TGGCCA 1 cut(s) 107
MluNI TGGCCA 1 cut(s) 107
MlyI GAGTC 9 cut(s) 84, 382, 889, 1100, 1243, 1587, 2321, 2797, 2971
MmeI TCCRAC 4 cut(s) 406, 634, 1728, 2222
Mox20I TGGCCA 1 cut(s) 107
MroI TCCGGA 2 cut(s) 460, 3332
MroXI GAANNNNTTC 4 cut(s) 193, 2049, 2578, 3126
MscI TGGCCA 1 cut(s) 107
MslI CAYNNNNRTG 3 cut(s) 1540, 1917, 2481
Msp20I TGGCCA 1 cut(s) 107
MspA1I CMGCKG 3 cut(s) 977, 1054, 2729
MspCI CTTAAG 2 cut(s) 328, 1256
MspI CCGG 7 cut(s) 140, 336, 461, 1572, 2425, 3111, 3333
MspR9I CCNGG 9 cut(s) 141, 336, 513, 1032, 1246, 1573, 2426, 3111, 3404
MunI CAATTG 1 cut(s) 2274
Mva1269I GAATGC 2 cut(s) 1705, 2134
MvaI CCWGG 4 cut(s) 513, 1032, 1246, 3404
NciI CCSGG 5 cut(s) 141, 336, 1573, 2426, 3111
NcoI CCATGG 1 cut(s) 102
NdeI CATATG 1 cut(s) 2587
NlaIV GGNNCC 8 cut(s) 340, 1452, 2265, 2392, 3087, 3115, 3287, 3387
NmuCI GTSAC 5 cut(s) 466, 984, 2707, 2839, 3274
NspI RCATGY 3 cut(s) 2256, 2337, 2925
PaeI GCATGC 2 cut(s) 2256, 2337
PaeR7I CTCGAG 1 cut(s) 3254
PagI TCATGA 1 cut(s) 1411
PceI AGGCCT 3 cut(s) 511, 1427, 2784
PciI ACATGT 1 cut(s) 2921
PctI GAATGC 2 cut(s) 1705, 2134
PdmI GAANNNNTTC 4 cut(s) 193, 2049, 2578, 3126
PflMI CCANNNNNTGG 1 cut(s) 2854
PfoI TCCNGGA 1 cut(s) 1030
PleI GAGTC 9 cut(s) 84, 381, 889, 1099, 1243, 1586, 2320, 2797, 2970
PpsI GAGTC 9 cut(s) 84, 381, 889, 1099, 1243, 1586, 2320, 2797, 2970
Ppu21I YACGTR 1 cut(s) 1379
PpuMI RGGWCCY 1 cut(s) 18
PscI ACATGT 1 cut(s) 2921
PshBI ATTAAT 1 cut(s) 1803
Psp5II RGGWCCY 1 cut(s) 18
Psp6I CCWGG 4 cut(s) 511, 1030, 1244, 3402
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 4 cut(s) 511, 1030, 1244, 3402
PspN4I GGNNCC 8 cut(s) 340, 1452, 2265, 2392, 3087, 3115, 3287, 3387
PspPI GGNCC 8 cut(s) 18, 260, 1200, 1477, 1750, 2140, 3143, 3330
PspPPI RGGWCCY 1 cut(s) 18
PsrI GAACNNNNNNTAC 2 cut(s) 2034, 2066
PstI CTGCAG 2 cut(s) 117, 1449
PstNI CAGNNNCTG 3 cut(s) 806, 1193, 1313
PsuI RGATCY 2 cut(s) 1190, 1309
PvuII CAGCTG 3 cut(s) 977, 1054, 2729
RsaI GTAC 6 cut(s) 832, 1058, 2225, 2830, 3017, 3162
RsaNI GTAC 6 cut(s) 831, 1057, 2224, 2829, 3016, 3161
RseI CAYNNNNRTG 3 cut(s) 1540, 1917, 2481
SalI GTCGAC 1 cut(s) 3453
Sau96I GGNCC 8 cut(s) 18, 260, 1200, 1477, 1750, 2140, 3143, 3330
ScaI AGTACT 1 cut(s) 832
SchI GAGTC 9 cut(s) 84, 382, 889, 1100, 1243, 1587, 2321, 2797, 2971
ScrFI CCNGG 9 cut(s) 141, 336, 513, 1032, 1246, 1573, 2426, 3111, 3404
SduI GDGCHC 3 cut(s) 146, 343, 1504
SfcI CTRYAG 7 cut(s) 113, 244, 397, 795, 1445, 3207, 3495
Sfr274I CTCGAG 1 cut(s) 3254
SinI GGWCC 5 cut(s) 18, 1477, 1750, 3143, 3330
SlaI CTCGAG 1 cut(s) 3254
SmiMI CAYNNNNRTG 3 cut(s) 1540, 1917, 2481
SmlI CTYRAG 5 cut(s) 328, 453, 1256, 2495, 3254
SmoI CTYRAG 5 cut(s) 328, 453, 1256, 2495, 3254
SnaBI TACGTA 1 cut(s) 1379
SphI GCATGC 2 cut(s) 2256, 2337
SseBI AGGCCT 3 cut(s) 511, 1427, 2784
SsiI CCGC 6 cut(s) 94, 1062, 1198, 1286, 1849, 1899
SspI AATATT 2 cut(s) 1528, 1649
SspMI CTAG 6 cut(s) 363, 420, 1152, 1182, 1595, 2340
StuI AGGCCT 3 cut(s) 511, 1427, 2784
StyD4I CCNGG 9 cut(s) 139, 334, 511, 1030, 1244, 1571, 2424, 3109, 3402
StyI CCWWGG 3 cut(s) 102, 2058, 2353
TaaI ACNGT 6 cut(s) 248, 601, 1078, 2248, 2632, 3241
TaiI ACGT 5 cut(s) 242, 269, 307, 468, 1381
TaqII GACCGA 1 cut(s) 2756
TatI WGTACW 4 cut(s) 830, 2223, 3015, 3160
TauI GCSGC 2 cut(s) 1201, 1901
TseFI GTSAC 5 cut(s) 466, 984, 2707, 2839, 3274
Tsp45I GTSAC 5 cut(s) 466, 984, 2707, 2839, 3274
TspGWI ACGGA 1 cut(s) 3457
Van91I CCANNNNNTGG 1 cut(s) 2854
Vha464I CTTAAG 2 cut(s) 328, 1256
VpaK11BI GGWCC 5 cut(s) 18, 1477, 1750, 3143, 3330
VspI ATTAAT 1 cut(s) 1803
XapI RAATTY 3 cut(s) 970, 1471, 2876
XbaI TCTAGA 2 cut(s) 419, 2339
XceI RCATGY 3 cut(s) 2256, 2337, 2925
XcmI CCANNNNNNNNNTGG 3 cut(s) 1242, 1647, 3296
XhoI CTCGAG 1 cut(s) 3254
XmiI GTMKAC 2 cut(s) 903, 3454
XmnI GAANNNNTTC 4 cut(s) 193, 2049, 2578, 3126
XspI CTAG 6 cut(s) 363, 420, 1152, 1182, 1595, 2340
ZrmI AGTACT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.