Rw6G039260

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0006256
Physical Location & Seq
Forward (+)
63294141 .. 63305385
11245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G039260.1

Sequence Viewer

Length: 3540 bp
ATGATTCATTTCCACAGGACCTTCTCATTGACAGCTCCTTCTTCCTCTTCATTCATGTTCTCATACCCTTCAACTTCTTCGGCTCTCAGACTCCGCCTCTCCATGGCCAAGCTGCAGTGTAGGGCGTTTGAGTATTATCCGGGCACTTGCTTGCCGTCTTCTCCAGTTTTGACATTGAATAATTACCGAAACTATTCCCAGCTTAGGTTTAGAAAATCCAGAGTTTCTTGCATGATCACGTCCTACGGTGCGAAAGTTGGGCCGACTTTTGCTCCTTTGAGAAGAAGTGGTGATGGATTTCAACGTGCTTTTAGCATTGATTCACACTTAAGACCGGGTGCCCTTAATGCTTTGGGGACTGCTAATGTTATGAGTCGTGGGTTTTTTGGAAATGGCTGTAGATCAAGTGTATTGGATTCTAGAGTTGGAGATTTTTCCAAGAGGTTTTTGTCTCAAGTTCCGGACGTGACAAGTAAAGTAGAGAATGTGGGCTCCCAAGATGGCAGGAGGCCTGGAAATAGAAAATGGTTCAAAATAATGATTAGCAATCGCAAGAAATTGAAAAAGGCATTGGCTACTCAAAGTGAAACTGAAGCTACCGTTTCGAGCATCTCCAAAGATGCTGATGTAAAATTTTCAAATGAAAAGCAGGTTGGAAGTGAGAAGGAGGATGAGAAGCTTGGATCATCAACTTCAGATTCGCCTATAAGCAAAACTAAAGAGGTCTCTAAACCAAAAGCAAAAAAGAGGTCGAAAAGCAAGAAAACTAAAGAACAAGATTCTGCTGCTAATGCTATAGCAGAAGCTGTTTCGACACAAAGTTCTGAAAGTACTTCTCAAACAAAGAAATCTGGCAGGAACAAAAAAGGAGCAAAGTCTGCACAGGATTCAGAGGTTATCTTTCATCTGAACGCTACTATGAAGACTCCAGTAGACCGTGCTGATAGTTCAACAACAAAACCCCAGAGAAACAAGAAAGGTGAGAAGGAAAAATCTATAAATTCAGCTGCCAAGTCACCACAGAAACTTATACCACAAGAGATGGGAAAATTAAAACCTCCTGGAAAAATGACAACGAAGCAGCTGTACCCCGCAACTGGGAAAACTATTGTGGTGGTGGAGTCTGTTACAAAGGCGAAGGTTATTCAGGGTTATCTTGGTGATATGTTTGAAGTGCTGCCTAGCTATGGTCATGTGAGGGACTTGGCAGCTAGGTCAGGATCTGTGCGGCCCGATGATGATTTTAGTATGGTGTGGGAAGTTCCATCTGCTGCCTGGACTCATCTTAAGAGCATAAAGGTCGCCCTAAGTGGTGCAGAAAATCTTATCCTTGCATCAGATCCTGATCGTGAAGGAGAGGCTATTGCTTGGCATATTATTGAGATGCTGCAACAACAGGATGCTTTACGTAAAGATATTTATGTAGCAAGGGTTGTCTTTCATGAAATCACTGAGGCCTCCATCAAAAGTGCTCTGCAGGCTCCCAGAGAAATTGATCAAAATTTGGTCCATGCTTACCTTGCACGGCGTGCTCTTGATTATTTGATCGGATTTAATATTTCACCGTTGCTATGGAGGAAACTGCCAAGTTGCCAGTCTGCCGGGCGAGTCCAATCTGCTGCACTAGCTCTTGTATGCGACAGGGAAATGGAAATCGACAGATTTAAACCACAAGAATATTGGACTATTGATATTGAATTGAACAAAAAAGAACCAGATTCATCAGTGAATGCCATATCCTTGCCAGCGCACTTGACCCATTTTGATTCCAAAAAGTTGGACCAATTTTCTATCAGTTCTCATACAGAGGCAAAAGATATTGAGCAGCAAATTAATTCAGAAGCTTTTAAAGTTATCAGCTCTAAGAGAAACAAAATGCGGAAGAACCCTCCTACGCCTTATATAACTTCAACACTTCAGCAGGATGCCGCAAACAAATTACATTTCAGTGCAACATATACAATGAAGCTTGCACAGAAGCTTTATGAGGGGGTTCAATTATCTGATGGCAAGTCAACGGGTTTGATCACATACACCAGAACAGATGGACTACACATTTCGGATGAAGCAGTAGAAGGTGTTCGCTCCTTGGTCATCGAAAGGTATGGACAGAACTTTGCATCAGCTACTCTACGCAAGTTTTTTAAGAAGGTGAAGAATGCTCAGGAGGCCCATGAAGCAATCAGACCTACTGATGTTCAAAGATTTCCATCGTTGCTCAGAGGGATACTTGATGAAGATTCATTGAAGTTGTACACTCTTATTTGGTGTCGGACAGTTGCATGCCAAATGGAGCCTGCTACAATTGAGCAGATACAACTTGATATTGGAAATGCTGGTGAGTCCATTGTTTTCCGATCTGCATGCTCAAGAGTTCAGTTCCTTGGATATCAGGCAGTTTTTCAGGACATGGAAGCTGGAGCCGTCAAATATAAAGAAAATGAAGGGATCAACCGGGATGAAGCTTTTGCACTTCTTGATTCACTGAAGGTTGGCAGTCCAGTGCATCTTGGTGAAGTGGAACTCAAGCAACACCATACCCAACCACCTCCACGCTACTCTGAGGCATCATTGGTTAAGAAATTAGAGGAGCTTGGGATTGGAAGACCTTCAACATATGCAAGCACTATAAAGGTTTTACAGGACAGAAACTATCTGACAGTGAAAAGCCGTGTTTTACATCCAGAGTTTCGTGGTCGTATGGTGTCAGCATTTCTCTGCCACCATTTTTCTGAGGTCACAGATTATAGCTTCACAGCTGATATGGAGACCGAGCTTGATAATGTCTCTGCTGGCTTAACTGAATGGAAAGGCCTCCTAAGAGATTACTGGACTCGATTCAGTGGTTATTGTGAGCGTACCAGTAATGTGACTATCCATCAGGTGGAGAAAATGATGGAAAAAAAATTTGGAGACTTCTTGTTTGCTACTCTCCCTGATCAAAGCAGGACATGTCCAAGCTGTAATGAAGGAACTTTGATCTTCAAAGTGAGTCGATTCGGTGCAGGCTATTTCATAGGTTGTGATACACACCCAAAGTGCAAGTACATTGCTAAGACATTGTATGGTAATGAAGAAGAAGACGACAATACTCAGAAGAACAACAGGGTGGAGGAACCAAAATTGTTAGGTTTATCTCCCGGTTCCAATGAAAAGGTTCTTTTGAAGACTGGTCCTTATGGATCGTATGTACAACTTGGTGAAGACAGGAAGGGATACTCACCTAAAAGAGCCTCTATAGCACATATCAAGGATGTGAACTCAGTAACCGTTGAAGTTGCTCTCGAGTTGATGCGATATCCTGTGACATTGGGGAACCATCCGAAGGATGGACTACCAGTGATTTTAAAGATTGCAAGGTCCGGATATATAGTTAAACATCGACGCACAATGGCTTCTCTAACAAAGAATCAGGAACCTAATAAATTAACCCTGGAGGATGCATTGGAACTTTTGTCTGGGAAAAATACACGGAAAATTGGTCGACCTAAGGGAAAATCAAAGGTTGAAGAAAAAGGAGAAGCTATAGAAGCCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topoisom_bac Toprim Toprim_C_rpt zf-C4_Topoisom

Protein Analysis

1179

Amino Acids

131.56

Weight (kDa)

9.39

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Toprim PF01751 369 - 482 1.5e-16 Toprim domain
Topoisom_bac PF01131 498 - 939 5.9e-112 DNA topoisomerase
Zn_ribbon_Top1 PF01396 983 - 1019 6.8e-09 Topoisomerase DNA binding C4 zinc finger
Toprim_C_rpt PF13368 1047 - 1101 1e-08 Topoisomerase C-terminal repeat
Toprim_C_rpt PF13368 1107 - 1158 1.1e-07 Topoisomerase C-terminal repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 640
AccB1I GGYRCC 1 cut(s) 338
AccB7I CCANNNNNTGG 1 cut(s) 2884
AccI GTMKAC 2 cut(s) 933, 3484
AccIII TCCGGA 2 cut(s) 460, 3362
AciI CCGC 5 cut(s) 94, 1092, 1228, 1879, 1929
AclWI GGATC 5 cut(s) 691, 1228, 1334, 2456, 3190
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 4 cut(s) 632, 1000, 1501, 2906
AcuI CTGAAG 4 cut(s) 612, 678, 1901, 2507
AdeI CACNNNGTG 1 cut(s) 1529
AfaI GTAC 6 cut(s) 832, 1088, 2255, 2860, 3047, 3192
AfiI CCNNNNNNNGG 8 cut(s) 103, 204, 1097, 1098, 1187, 2884, 3325, 3329
AflII CTTAAG 2 cut(s) 328, 1286
AflIII ACRYGT 1 cut(s) 2951
AjiI CACGTC 2 cut(s) 240, 466
AjnI CCWGG 4 cut(s) 511, 1060, 1274, 3432
AjuI GAANNNNNNNTTGG 6 cut(s) 554, 586, 636, 668, 2892, 2924
Alw21I GWGCWC 2 cut(s) 1474, 1534
Alw26I GTCTC 5 cut(s) 456, 730, 2762, 2791, 2907
AlwI GGATC 5 cut(s) 691, 1228, 1334, 2456, 3190
AlwNI CAGNNNCTG 3 cut(s) 806, 1223, 1343
Ama87I CYCGRG 1 cut(s) 3284
Aor13HI TCCGGA 2 cut(s) 460, 3362
AoxI GGCC 7 cut(s) 105, 260, 509, 1229, 1455, 2169, 2812
ApoI RAATTY 4 cut(s) 632, 1000, 1501, 2906
ArsI GACNNNNNNTTYG 4 cut(s) 734, 766, 2906, 2938
AseI ATTAAT 1 cut(s) 1833
Asp700I GAANNNNTTC 4 cut(s) 193, 2079, 2608, 3156
AspLEI GCGC 1 cut(s) 1750
AspS9I GGNCC 8 cut(s) 18, 260, 1230, 1507, 1780, 2170, 3173, 3360
AsuC2I CCSGG 5 cut(s) 141, 336, 1603, 2456, 3141
AvaI CYCGRG 1 cut(s) 3284
AvaII GGWCC 5 cut(s) 18, 1507, 1780, 3173, 3360
AxyI CCTNAGG 1 cut(s) 3489
BaeGI GKGCMC 2 cut(s) 146, 343
BalI TGGCCA 1 cut(s) 107
BanI GGYRCC 1 cut(s) 338
BanII GRGCYC 1 cut(s) 494
BarI GAAGNNNNNNTAC 2 cut(s) 1070, 1102
BbsI GAAGAC 6 cut(s) 150, 929, 2611, 3087, 3173, 3210
Bbv12I GWGCWC 2 cut(s) 1474, 1534
BceAI ACGGC 4 cut(s) 139, 1541, 2408, 2655
BcgI CGANNNNNNTGC 4 cut(s) 1282, 1316, 2346, 2380
BciT130I CCWGG 4 cut(s) 513, 1062, 1276, 3434
BciVI GTATCC 2 cut(s) 2220, 3209
BclI TGATCA 4 cut(s) 234, 1495, 2025, 2938
BcnI CCSGG 5 cut(s) 141, 336, 1603, 2456, 3141
BcoDI GTCTC 5 cut(s) 456, 730, 2762, 2791, 2907
BfaI CTAG 4 cut(s) 420, 1182, 1212, 1625
BfmI CTRYAG 6 cut(s) 113, 397, 795, 1475, 3237, 3525
BfrI CTTAAG 2 cut(s) 328, 1286
BfuAI ACCTGC 1 cut(s) 640
BfuI GTATCC 2 cut(s) 2220, 3209
BmcAI AGTACT 1 cut(s) 832
Bme1390I CCNGG 9 cut(s) 141, 336, 513, 1062, 1276, 1603, 2456, 3141, 3434
Bme18I GGWCC 5 cut(s) 18, 1507, 1780, 3173, 3360
BmeT110I CYCGRG 1 cut(s) 3284
BmgBI CACGTC 2 cut(s) 240, 466
BmgT120I GGNCC 8 cut(s) 18, 260, 1230, 1507, 1780, 2170, 3173, 3360
BmiI GGNNCC 9 cut(s) 340, 493, 1482, 2295, 2422, 3117, 3145, 3317, 3417
BmrFI CCNGG 9 cut(s) 141, 336, 513, 1062, 1276, 1603, 2456, 3141, 3434
BmrI ACTGGG 1 cut(s) 1107
BmuI ACTGGG 1 cut(s) 1107
BpiI GAAGAC 6 cut(s) 150, 929, 2611, 3087, 3173, 3210
BpmI CTGGAG 4 cut(s) 147, 912, 2439, 3455
Bpu10I CCTNAGC 2 cut(s) 203, 2163
BpuEI CTTGAG 3 cut(s) 438, 2353, 2510
BpuMI CCSGG 5 cut(s) 141, 336, 1603, 2456, 3141
BsaAI YACGTR 1 cut(s) 1409
BsaBI GATNNNNATC 2 cut(s) 1344, 2209
BsaI GGTCTC 2 cut(s) 730, 2762
BsaJI CCNNGG 4 cut(s) 102, 2088, 2383, 3432
BsaWI WCCGGW 2 cut(s) 460, 3362
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 8 cut(s) 103, 204, 1097, 1098, 1187, 2884, 3325, 3329
Bse1I ACTGG 9 cut(s) 164, 929, 1102, 1594, 2501, 2834, 2862, 3175, 3338
Bse21I CCTNAGG 1 cut(s) 3489
Bse3DI GCAATG 1 cut(s) 3048
Bse8I GATNNNNATC 2 cut(s) 1344, 2209
BseAI TCCGGA 2 cut(s) 460, 3362
BseBI CCWGG 4 cut(s) 513, 1062, 1276, 3434
BseDI CCNNGG 4 cut(s) 102, 2088, 2383, 3432
BseJI GATNNNNATC 2 cut(s) 1344, 2209
BseLI CCNNNNNNNGG 8 cut(s) 103, 204, 1097, 1098, 1187, 2884, 3325, 3329
BseMI GCAATG 1 cut(s) 3048
BseMII CTCAG 8 cut(s) 100, 1443, 2177, 2233, 2553, 2724, 3107, 3276
BseNI ACTGG 9 cut(s) 164, 929, 1102, 1594, 2501, 2834, 2862, 3175, 3338
BseRI GAGGAG 1 cut(s) 2603
BseSI GKGCMC 2 cut(s) 146, 343
BseYI CCCAGC 1 cut(s) 198
BsgI GTGCAG 4 cut(s) 864, 1335, 1605, 3024
BshFI GGCC 7 cut(s) 107, 262, 511, 1231, 1457, 2171, 2814
BshNI GGYRCC 1 cut(s) 338
BsiHKAI GWGCWC 2 cut(s) 1474, 1534
BsiHKCI CYCGRG 1 cut(s) 3284
BsiSI CCGG 7 cut(s) 140, 335, 461, 1602, 2455, 3141, 3363
BslFI GGGAC 2 cut(s) 370, 1214
BslI CCNNNNNNNGG 8 cut(s) 103, 204, 1097, 1098, 1187, 2884, 3325, 3329
BsmAI GTCTC 5 cut(s) 456, 730, 2762, 2791, 2907
BsmFI GGGAC 2 cut(s) 370, 1214
BsmI GAATGC 2 cut(s) 1735, 2164
BsnI GGCC 7 cut(s) 107, 262, 511, 1231, 1457, 2171, 2814
Bso31I GGTCTC 2 cut(s) 730, 2762
BsoBI CYCGRG 1 cut(s) 3284
Bsp1286I GDGCHC 5 cut(s) 146, 343, 494, 1474, 1534
Bsp13I TCCGGA 2 cut(s) 460, 3362
Bsp1407I TGTACA 2 cut(s) 2253, 3190
Bsp19I CCATGG 1 cut(s) 102
BspACI CCGC 5 cut(s) 94, 1092, 1228, 1879, 1929
BspANI GGCC 7 cut(s) 107, 262, 511, 1231, 1457, 2171, 2814
BspCNI CTCAG 8 cut(s) 99, 1444, 2176, 2232, 2554, 2725, 3106, 3275
BspEI TCCGGA 2 cut(s) 460, 3362
BspHI TCATGA 1 cut(s) 1441
BspLI GGNNCC 9 cut(s) 340, 493, 1482, 2295, 2422, 3117, 3145, 3317, 3417
BspMAI CTGCAG 2 cut(s) 117, 1479
BspMI ACCTGC 1 cut(s) 640
BspPI GGATC 5 cut(s) 691, 1228, 1334, 2456, 3190
BspT107I GGYRCC 1 cut(s) 338
BspTI CTTAAG 2 cut(s) 328, 1286
BspTNI GGTCTC 2 cut(s) 730, 2762
BsrDI GCAATG 1 cut(s) 3048
BsrGI TGTACA 2 cut(s) 2253, 3190
BsrI ACTGG 9 cut(s) 164, 929, 1102, 1594, 2501, 2834, 2862, 3175, 3338
BssECI CCNNGG 4 cut(s) 102, 2088, 2383, 3432
BssT1I CCWWGG 3 cut(s) 102, 2088, 2383
Bst2UI CCWGG 4 cut(s) 513, 1062, 1276, 3434
Bst4CI ACNGT 7 cut(s) 248, 601, 938, 1566, 2278, 2662, 3271
Bst6I CTCTTC 1 cut(s) 52
BstAFI CTTAAG 2 cut(s) 328, 1286
BstAPI GCANNNNNTGC 2 cut(s) 878, 1529
BstAUI TGTACA 2 cut(s) 2253, 3190
BstBAI YACGTR 1 cut(s) 1409
BstDSI CCRYGG 1 cut(s) 102
BstHHI GCGC 1 cut(s) 1750
BstMAI GTCTC 5 cut(s) 456, 730, 2762, 2791, 2907
BstNI CCWGG 4 cut(s) 513, 1062, 1276, 3434
BstNSI RCATGY 3 cut(s) 2286, 2367, 2955
BstSCI CCNGG 9 cut(s) 139, 334, 511, 1060, 1274, 1601, 2454, 3139, 3432
BstSFI CTRYAG 6 cut(s) 113, 397, 795, 1475, 3237, 3525
BstSLI GKGCMC 2 cut(s) 146, 343
BstSNI TACGTA 1 cut(s) 1409
BstV2I GAAGAC 6 cut(s) 150, 929, 2611, 3087, 3173, 3210
BstX2I RGATCY 2 cut(s) 1220, 1339
BstXI CCANNNNNNTGG 1 cut(s) 1777
BstYI RGATCY 2 cut(s) 1220, 1339
Bsu36I CCTNAGG 1 cut(s) 3489
BsuI GTATCC 2 cut(s) 2220, 3209
BsuRI GGCC 7 cut(s) 107, 262, 511, 1231, 1457, 2171, 2814
BtgI CCRYGG 1 cut(s) 102
BtrI CACGTC 2 cut(s) 240, 466
BtsI GCAGTG 1 cut(s) 122
BtsIMutI CAGTG 9 cut(s) 122, 1449, 1731, 1954, 2483, 2508, 2667, 2848, 3345
BveI ACCTGC 1 cut(s) 640
CaiI CAGNNNCTG 3 cut(s) 806, 1223, 1343
CciI TCATGA 1 cut(s) 1441
CfoI GCGC 1 cut(s) 1750
Cfr13I GGNCC 8 cut(s) 18, 260, 1230, 1507, 1780, 2170, 3173, 3360
CseI GACGC 1 cut(s) 3393
Csp6I GTAC 6 cut(s) 831, 1087, 2254, 2859, 3046, 3191
CviQI GTAC 6 cut(s) 831, 1087, 2254, 2859, 3046, 3191
DraI TTTAAA 3 cut(s) 1666, 1849, 3348
DraIII CACNNNGTG 1 cut(s) 1529
EaeI YGGCCR 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 52
EarI CTCTTC 1 cut(s) 52
EciI GGCGGA 1 cut(s) 83
Eco105I TACGTA 1 cut(s) 1409
Eco130I CCWWGG 3 cut(s) 102, 2088, 2383
Eco147I AGGCCT 3 cut(s) 511, 1457, 2814
Eco24I GRGCYC 1 cut(s) 494
Eco31I GGTCTC 2 cut(s) 730, 2762
Eco32I GATATC 2 cut(s) 2390, 3299
Eco47I GGWCC 5 cut(s) 18, 1507, 1780, 3173, 3360
Eco57I CTGAAG 4 cut(s) 612, 678, 1901, 2507
Eco81I CCTNAGG 1 cut(s) 3489
Eco88I CYCGRG 1 cut(s) 3284
EcoO109I RGGNCCY 1 cut(s) 18
EcoRII CCWGG 4 cut(s) 511, 1060, 1274, 3432
EcoRV GATATC 2 cut(s) 2390, 3299
EcoT14I CCWWGG 3 cut(s) 102, 2088, 2383
EcoT22I ATGCAT 1 cut(s) 3445
EcoT38I GRGCYC 1 cut(s) 494
ErhI CCWWGG 3 cut(s) 102, 2088, 2383
FaqI GGGAC 2 cut(s) 370, 1214
FauI CCCGC 1 cut(s) 1099
FauNDI CATATG 1 cut(s) 2617
FbaI TGATCA 4 cut(s) 234, 1495, 2025, 2938
FblI GTMKAC 2 cut(s) 933, 3484
FriOI GRGCYC 1 cut(s) 494
FspBI CTAG 4 cut(s) 420, 1182, 1212, 1625
GlaI GCGC 1 cut(s) 1749
GsaI CCCAGC 1 cut(s) 202
GsuI CTGGAG 4 cut(s) 147, 912, 2439, 3455
HaeIII GGCC 7 cut(s) 107, 262, 511, 1231, 1457, 2171, 2814
HapII CCGG 7 cut(s) 140, 335, 461, 1602, 2455, 3141, 3363
HgaI GACGC 1 cut(s) 3393
HhaI GCGC 1 cut(s) 1750
Hin6I GCGC 1 cut(s) 1748
HinP1I GCGC 1 cut(s) 1748
HincII GTYRAC 2 cut(s) 2016, 3485
HindII GTYRAC 2 cut(s) 2016, 3485
HindIII AAGCTT 5 cut(s) 677, 1842, 1967, 1979, 2463
HpaII CCGG 7 cut(s) 140, 335, 461, 1602, 2455, 3141, 3363
Hpy166II GTNNAC 5 cut(s) 934, 2016, 2256, 3259, 3485
Hpy8I GTNNAC 5 cut(s) 934, 2016, 2256, 3259, 3485
Hpy99I CGWCG 1 cut(s) 3387
HpyCH4III ACNGT 7 cut(s) 248, 601, 938, 1566, 2278, 2662, 3271
HpyCH4IV ACGT 4 cut(s) 239, 304, 465, 1408
HpySE526I ACGT 4 cut(s) 239, 304, 465, 1408
HspAI GCGC 1 cut(s) 1748
Kpn2I TCCGGA 2 cut(s) 460, 3362
Ksp22I TGATCA 4 cut(s) 234, 1495, 2025, 2938
LmnI GCTCC 9 cut(s) 40, 277, 497, 869, 1486, 2090, 2293, 2420, 2590
MaeI CTAG 4 cut(s) 420, 1182, 1212, 1625
MaeII ACGT 4 cut(s) 239, 304, 465, 1408
MaeIII GTNAC 7 cut(s) 466, 1014, 1126, 2737, 2869, 3265, 3304
MfeI CAATTG 1 cut(s) 2304
MflI RGATCY 2 cut(s) 1220, 1339
MhlI GDGCHC 5 cut(s) 146, 343, 494, 1474, 1534
MlsI TGGCCA 1 cut(s) 107
MluNI TGGCCA 1 cut(s) 107
MlyI GAGTC 9 cut(s) 84, 382, 919, 1130, 1273, 1617, 2351, 2827, 3001
MmeI TCCRAC 4 cut(s) 406, 634, 1758, 2252
Mox20I TGGCCA 1 cut(s) 107
Mph1103I ATGCAT 1 cut(s) 3445
MroI TCCGGA 2 cut(s) 460, 3362
MroXI GAANNNNTTC 4 cut(s) 193, 2079, 2608, 3156
MscI TGGCCA 1 cut(s) 107
MslI CAYNNNNRTG 2 cut(s) 1947, 2511
Msp20I TGGCCA 1 cut(s) 107
MspA1I CMGCKG 3 cut(s) 1007, 1084, 2759
MspCI CTTAAG 2 cut(s) 328, 1286
MspI CCGG 7 cut(s) 140, 335, 461, 1602, 2455, 3141, 3363
MspR9I CCNGG 9 cut(s) 141, 336, 513, 1062, 1276, 1603, 2456, 3141, 3434
MunI CAATTG 1 cut(s) 2304
Mva1269I GAATGC 2 cut(s) 1735, 2164
MvaI CCWGG 4 cut(s) 513, 1062, 1276, 3434
NciI CCSGG 5 cut(s) 141, 336, 1603, 2456, 3141
NcoI CCATGG 1 cut(s) 102
NdeI CATATG 1 cut(s) 2617
NlaIV GGNNCC 9 cut(s) 340, 493, 1482, 2295, 2422, 3117, 3145, 3317, 3417
NmuCI GTSAC 5 cut(s) 466, 1014, 2737, 2869, 3304
NsiI ATGCAT 1 cut(s) 3445
NspI RCATGY 3 cut(s) 2286, 2367, 2955
PaeI GCATGC 2 cut(s) 2286, 2367
PaeR7I CTCGAG 1 cut(s) 3284
PagI TCATGA 1 cut(s) 1441
PceI AGGCCT 3 cut(s) 511, 1457, 2814
PciI ACATGT 1 cut(s) 2951
PctI GAATGC 2 cut(s) 1735, 2164
PdmI GAANNNNTTC 4 cut(s) 193, 2079, 2608, 3156
PflMI CCANNNNNTGG 1 cut(s) 2884
PfoI TCCNGGA 1 cut(s) 1060
PleI GAGTC 9 cut(s) 84, 381, 919, 1129, 1273, 1616, 2350, 2827, 3000
PpsI GAGTC 9 cut(s) 84, 381, 919, 1129, 1273, 1616, 2350, 2827, 3000
Ppu21I YACGTR 1 cut(s) 1409
PpuMI RGGWCCY 1 cut(s) 18
PscI ACATGT 1 cut(s) 2951
PshBI ATTAAT 1 cut(s) 1833
Psp5II RGGWCCY 1 cut(s) 18
Psp6I CCWGG 4 cut(s) 511, 1060, 1274, 3432
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 4 cut(s) 511, 1060, 1274, 3432
PspN4I GGNNCC 9 cut(s) 340, 493, 1482, 2295, 2422, 3117, 3145, 3317, 3417
PspPI GGNCC 8 cut(s) 18, 260, 1230, 1507, 1780, 2170, 3173, 3360
PspPPI RGGWCCY 1 cut(s) 18
PsrI GAACNNNNNNTAC 2 cut(s) 2064, 2096
PstI CTGCAG 2 cut(s) 117, 1479
PstNI CAGNNNCTG 3 cut(s) 806, 1223, 1343
PsuI RGATCY 2 cut(s) 1220, 1339
PvuII CAGCTG 3 cut(s) 1007, 1084, 2759
RsaI GTAC 6 cut(s) 832, 1088, 2255, 2860, 3047, 3192
RsaNI GTAC 6 cut(s) 831, 1087, 2254, 2859, 3046, 3191
RseI CAYNNNNRTG 2 cut(s) 1947, 2511
SalI GTCGAC 1 cut(s) 3483
Sau96I GGNCC 8 cut(s) 18, 260, 1230, 1507, 1780, 2170, 3173, 3360
ScaI AGTACT 1 cut(s) 832
SchI GAGTC 9 cut(s) 84, 382, 919, 1130, 1273, 1617, 2351, 2827, 3001
ScrFI CCNGG 9 cut(s) 141, 336, 513, 1062, 1276, 1603, 2456, 3141, 3434
SduI GDGCHC 5 cut(s) 146, 343, 494, 1474, 1534
SfcI CTRYAG 6 cut(s) 113, 397, 795, 1475, 3237, 3525
Sfr274I CTCGAG 1 cut(s) 3284
SinI GGWCC 5 cut(s) 18, 1507, 1780, 3173, 3360
SlaI CTCGAG 1 cut(s) 3284
SmiMI CAYNNNNRTG 2 cut(s) 1947, 2511
SmlI CTYRAG 6 cut(s) 328, 453, 1286, 2368, 2525, 3284
SmoI CTYRAG 6 cut(s) 328, 453, 1286, 2368, 2525, 3284
SnaBI TACGTA 1 cut(s) 1409
SphI GCATGC 2 cut(s) 2286, 2367
SseBI AGGCCT 3 cut(s) 511, 1457, 2814
SsiI CCGC 5 cut(s) 94, 1092, 1228, 1879, 1929
SspI AATATT 2 cut(s) 1558, 1679
SspMI CTAG 4 cut(s) 420, 1182, 1212, 1625
StuI AGGCCT 3 cut(s) 511, 1457, 2814
StyD4I CCNGG 9 cut(s) 139, 334, 511, 1060, 1274, 1601, 2454, 3139, 3432
StyI CCWWGG 3 cut(s) 102, 2088, 2383
TaaI ACNGT 7 cut(s) 248, 601, 938, 1566, 2278, 2662, 3271
TaiI ACGT 4 cut(s) 242, 307, 468, 1411
TaqII GACCGA 1 cut(s) 2786
TatI WGTACW 4 cut(s) 830, 2253, 3045, 3190
TauI GCSGC 2 cut(s) 1231, 1931
TscAI CASTG 9 cut(s) 122, 1456, 1731, 1954, 2490, 2508, 2667, 2848, 3345
TseFI GTSAC 5 cut(s) 466, 1014, 2737, 2869, 3304
Tsp45I GTSAC 5 cut(s) 466, 1014, 2737, 2869, 3304
TspGWI ACGGA 1 cut(s) 3487
TspRI CASTG 9 cut(s) 122, 1456, 1731, 1954, 2490, 2508, 2667, 2848, 3345
Van91I CCANNNNNTGG 1 cut(s) 2884
Vha464I CTTAAG 2 cut(s) 328, 1286
VpaK11BI GGWCC 5 cut(s) 18, 1507, 1780, 3173, 3360
VspI ATTAAT 1 cut(s) 1833
XapI RAATTY 4 cut(s) 632, 1000, 1501, 2906
XbaI TCTAGA 1 cut(s) 419
XceI RCATGY 3 cut(s) 2286, 2367, 2955
XcmI CCANNNNNNNNNTGG 3 cut(s) 1272, 1677, 3326
XhoI CTCGAG 1 cut(s) 3284
XmiI GTMKAC 2 cut(s) 933, 3484
XmnI GAANNNNTTC 4 cut(s) 193, 2079, 2608, 3156
XspI CTAG 4 cut(s) 420, 1182, 1212, 1625
ZrmI AGTACT 1 cut(s) 832
Zsp2I ATGCAT 1 cut(s) 3445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.